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Abstract Background Coronavirus disease 2019 (COVID-19) caused by the enveloped RNA virus SARS-CoV-2 primarily affects the respiratory and gastrointestinal tracts. SARS-CoV-2 was isolated from fecal samples, and active viral replication was reported in human intestinal cells. The human gut also harbors an enormous amount of resident viruses (collectively known as the virome) that play a role in regulating host immunity and disease pathophysiology. Understanding gut virome perturbation that underlies SARS-CoV-2 infection and severity is an unmet need. Methods We enrolled 98 COVID-19 patients with varying disease severity (3 asymptomatic, 53 mild, 34 moderate, 5 severe, 3 critical) and 78 non-COVID-19 controls matched for gender and co-morbidities. All subjects had fecal specimens sampled at inclusion. Blood specimens were collected for COVID-19 patients at admission to test for inflammatory markers and white cell counts. Among COVID-19 cases, 37 (38%) patients had serial fecal samples collected 2 to 3 times per week from time of hospitalization until after discharge. Using shotgun metagenomics sequencing, we sequenced and profiled the fecal RNA and DNA virome. We investigated alterations and longitudinal dynamics of the gut virome in association with disease severity and blood parameters. Results Patients with COVID-19 showed underrepresentation of Pepper mild mottle virus (RNA virus) and multiple bacteriophage lineages (DNA viruses) and enrichment of environment-derived eukaryotic DNA viruses in fecal samples, compared to non-COVID-19 subjects. Such gut virome alterations persisted up to 30 days after disease resolution. Fecal virome in SARS-CoV-2 infection harbored more stress-, inflammation-, and virulence-associated gene encoding capacities including those pertaining to bacteriophage integration, DNA repair, and metabolism and virulence associated with their bacterial host. Baseline fecal abundance of 10 virus species (1 RNA virus, pepper chlorotic spot virus, and 9 DNA virus species) inversely correlated with disease COVID-19 severity. These viruses inversely correlated with blood levels of pro-inflammatory proteins, white cells, and neutrophils. Among the 10 COVID-19 severity-associated DNA virus species, 4 showed inverse correlation with age; 5 showed persistent lower abundance both during disease course and after disease resolution relative to non-COVID-19 subjects. Conclusions Both enteric RNA and DNA virome in COVID-19 patients were different from non-COVID-19 subjects, which persisted after disease resolution of COVID-19. Gut virome may calibrate host immunity and regulate severity to SARS-CoV-2 infection. Our observation that gut viruses inversely correlated with both severity of COVID-19 and host age may partly explain that older subjects are prone to severe and worse COVID-19 outcomes. Altogether, our data highlight the importance of human gut virome in severity and potentially therapeutics of COVID-19. Video Abstract. more...

Abstract Background The One-Child Policy led to the imbalance of the sex ratio at birth (SRB) in China. After that, Two-Child Policy was introduced and gradually liberalized at three stages. If both the husband and wife of one couple were the only child of their parents, they were allowed to have two children in policy (BTCP). If only one of them was the only child, they were allowed to have two children in policy (OTCP). The Universal Two-Child Policy (UTCP) allowed every couple to have two children. The objective of this study was to explore the changing trend of SRB at the stages of Two-Child Policy, to analyze the effect of population policy on SRB in terms of maternal age, delivery mode, parity, maternal education, delivery hospital, and to figure out what factors have greater impact on the SRB. Methods The data of the study came from Hebei Province Maternal Near Miss Surveillance System, covered the parturients delivered at 28 gestation weeks or more in 22 hospitals from January 1, 2013 to December 31, 2017. We compared the SRB at different policy stages, analyzed the relationship between the SRB and population policy by logistic regression analysis. Results Total 270,878 singleton deliveries were analyzed. The SRB, 1.084 at BTCP, 1.050 at OTCP, 1.047 at UTCP, declined rapidly (χ2 = 15.97, P more...

The physics of laser-plasma interaction is studied on the Shenguang III prototype laser facility under conditions relevant to inertial confinement fusion designs. A sub-millimeter-size underdense hot plasma is created by ionization of a low-density plastic foam by four high-energy (3.2 kJ) laser beams. An interaction beam is fired with a delay permitting evaluation of the excitation of parametric instabilities at different stages of plasma evolution. Multiple diagnostics are used for plasma characterization, scattered radiation, and accelerated electrons. The experimental results are analyzed with radiation hydrodynamic simulations that take account of foam ionization and homogenization. The measured level of stimulated Raman scattering is almost one order of magnitude larger than that measured in experiments with gasbags and hohlraums on the same installation, possibly because of a greater plasma density. Notable amplification is achieved in high-intensity speckles, indicating the importance of implementing laser temporal smoothing techniques with a large bandwidth for controlling laser propagation and absorption. more...

The corrosion resistance and semiconductors of passivation film on the surface of 316L stainless steel has been explored using linear polarization，Potentiostatic polarization electrochemical impedance spectroscopy (EIS) and Mott–Schottky analysis in 0,1 / mol/L Na2SO4 and 0,01 / mol/L HCl solution in the steady state condition. Based on the Mott-Schottky analysis, the density and thickness of the passivation film are related to the potential formed by the passivation film. The passivation film is an n-type semiconductor. There is no metal ion loss or cation vacancy remaining; when the film forming potential is 0,3 / V, The chemical reaction rate at the interface is slow, and the passivation film is denser than the passivation film formed at other potentials. more...

Brassinosteroids (Brs) have drawn wide attention due to their protective role against toxicity of heavy metals in plants. To better understand the role of Br in arsenic (As) and cadmium (Cd) uptake by rice plants, a hydroponic experiment was conducted to investigate the combined effect of 24-epibrassinolide (Br24) or 28-homobrassinolide (Br28) and iron plaque (IP) on As and Cd uptake and accumulation in rice seedlings. Six-week-old seedlings were sprayed with 0.2 or 0.02 μM Br24 or Br28 and grown in nutrient solution for 3 d, and then 20 or 60 mg Fe2+ dm-3 (Fe20 and Fe60) was used to induce root IP formation for 3 d. These seedlings with or without Br and with or without IP were exposed to solution containing 0.5 mg dm-3 AsIII or Cd for 9 d. The results showed that rice growth decreased when Br24 were applied, but it increased when combination of Br24 and IP was applied. Fe concentrations in dithionite-citratebicarbonate (DCB) extracts were increased after 0.2 or 0.02 μM Br24 application in the absence of IP, but decreased by Br24 in the presence of IP. In the absence of IP, As and Cd content in leaves was significantly reduced by 0.02 μM Br24 and 0.2 μM Br28, respectively. The As content in leaves was also reduced by the combination of 0.02 and 0.2 μM Br28 and IP, and the Cd content in leaves was reduced by the combined effect of 0.2 μM Br24 and IP. These results indicate that Br24 and Br28 could impede As and Cd accumulation, and the interactions between Br and IP may have a potential in restricting the transport of As and Cd into rice shoots. more...

Building extraction from high resolution remote sensing images is a hot research topic in the field of photogrammetry and remote sensing. However, the diversity and complexity of buildings make building extraction methods still face challenges in terms of accuracy, efficiency, and so on. In this study, a new building extraction framework based on MBI and combined with image segmentation techniques, spectral constraint, shadow constraint, and shape constraint is proposed. In order to verify the proposed method, worldview-2, GF-2, GF-1 remote sensing images covered Xiamen Software Park were used for building extraction experiments. Experimental results indicate that the proposed method improve the original MBI significantly, and the correct rate is over 86 %. Furthermore, the proposed framework reduces the false alarms by 42 % on average compared to the performance of the original MBI. more...

As key nuclear transcription factors, the ethylene-insensitive3/EIN3-like (EIN3/EIL) proteins play important roles in ethylene signal transduction pathway in various plants. In order to better understand the role of EIN3/EILs, one EIN3-like gene (designated ZmEIL1) was isolated from maize (Zea mays L.). The full-length cDNA of ZmEIL1 was 1 999 bp in length and encoded 647 amino acids. Sequence comparison of ZmEIL1 protein with other EIN3/EILs proteins revealed high conservation of five α-helices that could form a V-shaped cleft in a 3-D model, just like AtEIL3 in Arabidopsis thaliana. This protein showed transcriptional activation and activation domain located on the 507 - 647 amino acids in yeast. Furthermore, ZmEIL1 could interact with ZmERF1 in the yeast systems, which was downstream response factor in ethylene signal transduction pathway. Its mRNA could be highly induced in maize seedlings by ethephon and 1-methylcyclopropene treatments. Meanwhile, ZmEIL1 showed relatively high expression at 20 d after pollination in maize kernel. These results show that ZmEIL1 played an important role in the growth and development by participating in ethylene signalling pathway in maize. more...

The extreme cycles of flood and drought in the Poyang Lake catchment (China) place immense pressure on the region's water users and ecosystems. This study examines potential impacts of future climate change in the Poyang Lake catchment using the popular regional climate model, COSMO-CLM, and a distributed hydrological model, WATLAC. Near-future projections (2016–2035) indicate that the mean annual precipitation and temperature are expected to increase over the catchment, with the exception of some northern regions. Relative to the baseline period (1986–2005), the monthly mean precipitation is projected to increase in spring, summer and autumn (March-October), and to decrease in winter (November-February), with the most significant changes in September (62%) and January (−39%). Projected increases in monthly mean temperatures range from 0.3 to 1.4 °C, 0.2 to 0.7 °C, and 0.2 to 1.2 °C for Representative Concentration Pathways (RCP) climate scenarios RCP2.6, 4.5 and 8.5, respectively. Winter temperatures are expected to increase significantly regardless of the climate scenarios. WATLAC simulations indicate that future climate changes will lead to increased high flows in summer and reduced low flows in winter, in terms of both frequency and magnitude, suggesting a high likelihood of an increase in frequency and severity of flooding and droughts in the Poyang Lake catchment. more...

CO _2 laser cladding is a type of green remanufacturing technology, which is of many technical advantages in repairing and remanufacturing industry, especially for some large-scale key mining equipment or parts due to its characteristics of high quality, high efficiency and environmental protection (e.g. energy and material saving). In this paper, the chute plate of coal mining scraper is fixed and remanufactured by CO _2 laser cladding technique. Ni60, WC, Ni35, IG55 and other composite powders are selected to design and build the gradient functional structure for chute plate in order to improve the reproduced lifetime. A lot of high power low cost CO _2 laser cladding tests are carried out on the matrix material (AISI 1040 steel plate) of old attrite chute plate. The optical microscope, SEM, XRD, microhardness test and wear experiment are adopted to analyze the relationships among the laser cladding process, the overlaying composite material, gradient functional structure and mechanical properties of the remanufacturing scraper’s chute plate. The research results show that laser power and scanning speed are the dominant cladding process parameters, which have a significant influence on the geometric dimension (including width and height), dilution rate and hardness of the deposited layer. These composite powders (especially including the rare earth metals) are the key factor to form the gradient functional structure. The laser cladding Ni60 + WC/Ni35/AISI1040 composite gradient functional structure has a reasonable toughness and strength of the transition layer structure, and a high hardness and wear-resistant surface functional layer, so the fixed and remanufactured product has formed good ductile plasticity and wear resistance properties as a result of the gradient functional structure. The unique Ni60 + WC/Ni35/AISI1040 gradient functional structure makes sure that the chute plate of mining scraper has excellent comprehensive performance, which is satisfied with the service requirements of mechanical parts or equipment in the harsh working environment of the mining industry. This research work provides technological guidance for the fix and remanufacturing chute plate, and achieves the goal of low cost, high efficiency and long life reproduced chute plate of mining scraper. more...

Salt decreases the uptake of Zn and other minerals and causes nutritional disorders in plants. Zn is an essential micronutrient for all organisms and it is reasonable to hypothesize that Zn status is essential for maintaining salt tolerance in plants. In this study, the physiological and molecular mechanisms of Zn-based alleviation of salt stress in wheat seedlings were investigated. Our results indicate that sufficient Zn nutrition maintained antioxidative enzyme activities and decreased a reactive oxygen species over-accumulation in wheat seedlings. Our data also reveal that sufficient Zn nutrition improved the expression of Na+/H+ antiporter genes, TaSOS1 and TaNHX1, thereby decreasing the Na+ accumulation and subsequently improving salt tolerance in wheat seedlings. more...

Based on the elitist non-dominated sorting genetic algorithm (NSGA-II) for multi-objective optimization problems, an improved scheme with self-adaptive crossover and mutation operators is proposed to obtain good optimization performance in this paper. The performance of the improved NSGA-II is demonstrated with a set of test functions and metrics taken from the standard literature on multi-objective optimization. Combined with the HFSS solver, one pixel antenna with reconfigurable radiation patterns, which can steer its beam into six different directions (θDOA = ± 15°, ± 30°, ± 50°) with a 5 % overlapping impedance bandwidth (S11 less than 10 dB) and a realized gain over 6 dB, is designed by the proposed self-adaptive NSGA-II. more...

Abscisic acid (ABA) regulates plant responses to various environmental stresses. Oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the biosynthesis of ABA in higher plants. Using a homologous cloning approach, a NCED-like gene (designated as TaNCED1) was isolated from wheat (Triticum aestivum). It contained an open reading frame of 1 848 bp and encodes a peptide of 615 amino acids. Multiple sequence alignments showed that TaNCED1 shared high identity with NCEDs from other plants. Phylogenetic analysis revealed that TaNCED1 was most closely related to a barley HvNCED1 gene. The predicted 3D structure of TaNCED1 showed high similarity with other homologues. Southern blot analysis indicated that TaNCED1 was a single copy in the genome of wheat. TaNCED1 was differentially expressed in various organs and the expression was up-regulated by low temperature, drought, NaCl, and ABA. Heterologous expression of TaNCED1 in tobacco (Nicotiana tabacum) significantly improved its drought tolerance. Under drought treatment, TaNCED1-overexpressing transgenic tobacco plants exhibited higher germination rate, higher relative water content, content of soluble sugars and of ABA when compared with the wild type plants. more...

Cropping fields often have well-defined poor-performing patches due to spatial and temporal variability. In an attempt to increase crop performance on poor patches, spatially selective field operations may be performed by agricultural robotics to apply additional inputs with targeted requirements. This paper addresses the route planning problem for an agricultural robot that has to treat some poor-patches in a field with row crops, with respect to the minimization of the total non-working distance travelled during headland turnings and in-field travel distance. The traversal of patches in the field is expressed as the traversal of a mixed weighted graph, and then the problem of finding an optimal patch sequence is formulated as an asymmetric traveling salesman problem and solved by the partheno-genetic algorithm. The proposed method is applied on a cropping field located in Northwestern China. Research results show that by using optimum patch sequences, the total non-working distance travelled during headland turnings and in-field travel distance can be reduced. But the savings on the non-working distance inside the field interior depend on the size and location of patches in the field, and the introduction of agricultural robotics is beneficial to increase field efficiency. more...

The influence of second-generation products on the particle mass yield of β-caryophyllene ozonolysis was systematically tested and quantified. The approach was to vary the relative concentrations of first- and second-generation products by adjusting the concentration of ozone while observing changes in particle mass yield. For all wall-loss corrected organic particle mass concentrations Morg of this study (0.5 < Morg < 230 μg m−3), the data show that the particle-phase organic material was composed for the most part of second-generation products. For 0.5< Morg < 10 μg m−3, a range which overlaps with atmospheric concentrations, the particle mass yield was 10 to 20% and was not sensitive to ozone exposure, implying that the constituent molecules were rapidly produced at all investigated ozone exposures. In contrast, for Morg > 10 μg m−3 the particle mass yield increased to as high as 70% for the ultimate yield corresponding to the greatest ozone exposures. These differing dependencies on ozone exposure under different regimes of Morg are explained by a combination of the ozonolysis lifetimes of the first-generation products and the volatility distribution of the resulting second-generation products. First-generation products that have short lifetimes produce low-volatility second-generation products whereas first-generation products that have long lifetimes produce high-volatility second-generation products. The ultimate particle mass yield was defined by mass-based stoichiometric yields αi of α0 = 0.17 ± 0.05, α1 = 0.11 ± 0.17, and α2 = 1.03 ± 0.30 for corresponding saturation concentrations of 1, 10, and 100 μg m−3. Terms α0 and α1 had low sensitivity to the investigated range of ozone exposure whereas term α2 increased from 0.32 ± 0.13 to 1.03 ± 0.30 as the ozone exposure was increased. These findings potentially allow for simplified yet accurate parameterizations in air quality and climate models that seek to represent the ozonolysis particle mass yields of certain classes of biogenic compounds. more...

A subtropical old-growth forest was studied over a twelve-year period to investigate temporal and spatial fluctuations of biomass and stem fluxes under disturbances. Vegetations were categorized into three types according to disturbances caused by biotic and abiotic factors, including Castanopsis chinensis population, insect direct-influenced population, and insect indirect-influenced population according to disturbance scenarios. The biomass fluxes (growth and mortality) and stem fluxes (stem recruitment and mortality) were used to quantify population fluctuations. Annual average biomass growth rate was stable throughout the study while annual biomass mortality and stem fluxes increased consistently. C. chinensis population predominantly contributed to biomass fluxes of the community. Biomass and stem mortalities of insect direct-influenced population increased significantly during the whole study period (1992–2004). Results of indirect-influenced population showed that (1) the increase in biomass of the dominant species was well correlated between different intervals. Similar relationships were found in stem fluxes; (2) higher stem mortality occurred within the DBH range of 1 to 10 cm; (3) stem fluxes in the canopy gaps were remarkably higher than those in closed canopy. more...