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Background There is little information on the pharmacokinetics and pharmacodynamics of sacubitril/valsartan (SV) in patients undergoing peritoneal dialysis (PD) complicated with hypertension or heart failure (HF). This study was designed to evaluate the pharmacokinetics and pharmacodynamics of SV in PD patients with complications of hypertension or HF. Methods This was an open-label and cross-sectional study investigating PD patients diagnosed with hypertension or New York Heart Association Class II–IV HF. The concentrations of valsartan, sacubitril and sacubitrilat (LBQ657) were measured by ultra-performance liquid chromatography tandem mass spectrometry in plasma, urine and peritoneal dialysate samples. Pharmacodynamics were evaluated by comparing changes in mean sitting systolic blood pressure (msSBP), mean sitting diastolic blood pressure (msDBP), mean sitting heart rate, N-terminal-pro B-type natriuretic peptide (NT-proBNP) and left ventricular ejection fraction (LVEF). Results Forty patients with PD were enrolled including 27 (67.5%) patients with hypertension, 4 (10%) patients with HF and 9 (22.5%) patients with both hypertension and HF. This study included three treatment cohorts: 50 mg twice daily (BID), 100 mg once daily and 100 mg BID. The plasma maximum drug concentrations in the 100 mg BID group were 1995 ± 1499 ng/mL for valsartan, 171 ± 148 ng/mL for sacubitril and 13 686 ± 7418 ng/mL for LBQ657. The 24-h recovery rate of LBQ657 was 3.77% in urine and 2.23% in peritoneal dialysate. After taking SV, msSBP and msDBP decreased by 19.25 ± 10.32 mmHg and 10.10 ± 8.00 mmHg from baseline, respectively. NT-proBNP decreased by 1436.50 (0.00–18 198.00) from baseline, while LVEF increased by 5.00 (–0.25 to 9.25) from baseline after SV treatment. Conclusions PD and residual renal function contributed only to a minor degree to the elimination of LBQ657. Additionally, a dose of 100 mg BID SV is safe and effective in patients with PD with complications of hypertension or HF.

A multiple pre-doping system with a looser structure displays small fractional volume expansion.

Carbon and nitrogen evolution and bacteria and fungi succession in two functional membrane-covered aerobic composting (FMCAC) systems and a conventional aerobic composting system were investigated. The micro-positive pressure in each FMCAC system altered the composting microenvironment, significantly increased the oxygen uptake rates of microbes (p0.05), and increased the abundance of cellulose- and hemicellulose-degrading microorganisms. Bacteria and fungi together influenced the conversion between carbon and nitrogen forms. FMCAC made the systems less anaerobic and decreased CH

Si is a promising high-capacity anode material; however, its practical implementation is hindered by its huge volume expansion, low initial Coulombic efficiency (ICE), poor cycling life, and high c...

Among biological fluids, cerebrospinal fluid (CSF) not only protects and support brain, but also plays a pivotal role in intracerebral interaction of various nano-drug carriers. However, it is still uncertain how protein corona from CSF affects the targeting capability of functionalized nanoparticles (NPs). So, two types of polystyrene NPs, including PEGylated polystyrene NPs (PN) and transferrin (Tf)-modified PN (PT), were used to obtain protein corona-coated NPs, by incubating with CSF in vivo and in vitro. Strikingly, both the corona-coated NPs recovered in vivo and in vitro completely lost their active targeting characteristics towards bEnd.3 and C6 cells. Charge-, clathrin- and energy-mediated endocytosis contributed to the improved uptake efficiency of PT, whereas this enhancement in uptake of PT was disappeared after the formation of CSF protein corona. Moreover, serum albumin, which were found both in vivo and in vitro CSF corona, could mediate and facilitate the internalization of corona-coated NPs. Overall, these results have distinctly confirmed that the formation of CSF protein corona could cause the loss of active targeting specificity by shielding the targeting groups on the surface of polystyrene NPs and alter their cellular uptake by other non-specific internalization pathways.

Current olivine structure LiFePO4 possesses poor conductivity, which makes it unsuitable for unparalleled battery applications. This issue can be solved by improved design concepts of binder systems. Herein, an original waterborne binder polymer with efficient functional groups is designed and fabricated for LiFePO4 cathodes of lithium-ion batteries (LIBs). Compared to universal lithiated ionomer binders, the introduction of the zwitterionic structure of the ionomer enhances the ionic conductivity by nearly 100-fold. LiFePO4 cathodes assembled with limited 1.5 wt% lithiated zwitterionic ionomer (LZI) maintain markedly stable cycling performances with 95.62% retention at 1C after 250 cycles. Also, LZI-cathodes preserve good integrity structures after cycling, as confirmed by morphology analysis. In sum, LZI-cathodes look promising for potential applications in LIBs due to their numerous advantageous characteristics.

Aerobic composting is a humification process accompanied by nitrogen loss. This study is the first research systematically investigating and elucidating the mechanism by which functional membrane-covered aerobic composting (FMCAC) reduces nitrogen loss and enhances humification. The variations in bioavailable organic nitrogen (BON) and humic substances (HSs) in different composting systems were quantitatively studied, and the functional succession patterns of fungal groups were determined by high-throughput sequencing and FUNGuild. The FMCAC improved oxygen utilization and pile temperature, increased BON by 29.95 %, reduced nitrogen loss by 34.00 %, and enhanced humification by 26.09 %. Meanwhile, the FMCAC increased the competitive advantage of undefined saprotroph and significantly reduced potential pathogenic fungi (0.10 %). Structural equation modeling indicated that undefined saprotroph facilitated the humification process by increasing the production of BON and storing BON in stable humic acid. Overall, the FMCAC increased the safety, stability, and quality of the final compost product.

In this study, the microbial mechanism of reducing methanogenesis during membrane-covered aerobic composting from solid dairy manure was investigated. An industrial-scale experiment was carried out to compare a static composting group (SC) and a forced aeration composting group (AC) with a semipermeable membrane-covered composting group (MC + AC). The results showed that the semipermeable membrane-covered could improve the oxygen utilization rate and inhibit the anaerobic bacterial genus Hydrogenispora and archaea order Methanobacteriales. During the membrane-covered period, the acetoclastic methanogenesis module in MC + AC, AC and SC decreased by 0.58%, 0.05% and 0.04%, respectively, and the cdhC gene in the acetoclastic pathway was found to be decreased by 65.51% only in MC + AC. Changes in methane metabolism pathways resulted in a 27.48% lower average methane concentration in MC + AC than in SC. Therefore, the semipermeable membrane-covered strategy can effectively reduce methane production during dairy manure aerobic composting by restricting the methanogenesis of the acetoclastic pathway.

This study investigated and assessed the effect of the functional-membrane covering technique (FMCT) on nitrogen succession during aerobic composting. By comparative experiments involving high-throughput sequencing and qPCR, nitrogen metabolism (including the ko00910 pathway and functional enzyme and gene abundances) was analyzed, and the nitrogen succession mechanism was identified. The FMCT created a micro-positive pressure, improved the aerobic conditions, and increased the oxygen utilization rate and temperature. This strongly affected the nitrogen metabolism pathway and down-regulated the nitrifying and denitrifying bacteria abundances. The FMCT up-regulated the relative abundance of glutamate dehydrogenase and down-regulated the absolute abundances of AOB and nxrA. This and the high temperature increased NH

Silicon/carbon (Si/C) composites have rightfully earned the attention as anode candidates for high-energy-density lithium-ion batteries (LIBs) owing to their advantageous capacity and superior cycling stability, yet their practical application remains a significant challenge. In this study, we report the large-scale synthesis of an intriguing micro/nanostructured pore-rich Si/C microsphere consisting of Si nanoparticles tightly immobilized onto a micron-sized cross-linked C matrix that is coated by a thin C layer (denoted P-Si/C@C) using a low-cost spray-drying approach and a chemical vapor deposition process with inorganic salts as pore-forming agents. The as-obtained P-Si/C@C composite has high porosity that provides sufficient inner voids to alleviate the huge volume expansion of Si. The outer smooth and robust C shells strengthen the stability of the entire structure and the solid-electrolyte interphase. Si nanoparticles embedded in a microsized cross-linked C matrix show excellent electrical conductivity and superior structural stability. By virtue of structural advantages, the as-fabricated P-Si/C@C anode displays a high initial Coulombic efficiency of 89.8%, a high reversible capacity of 1269.6 mAh g

This study investigated the effect of biochar on antibiotics and antibiotic resistance genes (ARGs) during aerobic composting of pig manure. First, the composition and content of antibiotics in the manure were determined qualitatively and quantitatively. Biochar promoted the degradation of these antibiotics (oxytetracycline, chlortetracycline, and tetracycline). The relative abundance (RA) of antibiotic-resistant bacteria carrying ARGs accounted for about 29.32% of the total bacteria. Firmicutes and Actinomycetes were dominant phylum-level bacteria at the early and late stages of composting, respectively. Biochar decreased the total RA of ARGs by 16.83%±4.10%. tetW and tetL, closely related to tetracycline resistance, were significantly diminished during aerobic composting, and biochar was able to promote this removal. Biochar enhanced RAs of Mycobacterium tuberculosis kasA mutant. RAs of ARGs related to antibiotic efflux pumps, such as baeS and arlS, remained at a high level. Conclusively, biochar promotes degradation of antibiotics and removal of ARGs.

A promoting effect of hydroxyls on silicon surface to the decomposition of electrolyte is discovered by comparing the performance of silicon-hydroxyl (Si-OH) and silicon-alkyl (Si-C3H7) electrodes....

Homogenous spatial distribution of fermentation characteristics, local anaerobic conditions, and large amounts of greenhouse gas (GHGs) emissions are common problems in large-scale aerobic composting systems. The aim of this study was to examine the effects of a semi-membrane covering on the spatial homogeneity and efficiency of fermentation in aerobic composting systems. In the covered group, the pile was covered with a semi-membrane, while in the non-covered group (control group), the pile was uncovered. The covered group entered the high-temperature period earlier and the spatial gradient difference in the group was smaller compared with the non-covered group. The moisture content loss ratio (5.91%) in the covered group was slower than that in the non-covered group (10.78%), and the covered group had a more homogeneous spatial distribution of water. The degradation rate of organic matter in the non-covered group (11.39%) was faster than that in the covered group (10.21%). The final germination index in the covered group (85.82%) was higher than that of the non-covered group (82.79%) and the spatial gradient difference in the covered group was smaller. Compared with the non-covered group, the oxygen consumption rate in the covered group was higher. The GHG emissions (by 30.36%) and power consumption in the covered group were reduced more significantly. The spatial microbial diversity of the non-covered group was greater compared with the covered group. This work shows that aerobic compost covered with a semi-membrane can improve the space homogeneity and efficiency of fermentation.

In this study, the effects of semipermeable membrane-covered on methane emissions and potential pathogens during industrial-scale composting of the solid fraction of dairy manure were investigated. The results showed that the oxygen concentration in the membrane-covered group (CT) was maintained above 10 %, and the cumulative methane emission in CT was99 % lower than that in the control group (CK). Microbial analysis showed that the bacterial genus Thermus and the fungal genus Mycothermus were dominant in CT, and the richness and diversity of the bacterial community were greater than those of the fungal community. At the end of the composting, the relative abundance of potential bacterial pathogens in CT was 32.59 % lower than that in CK, and the relative abundance of potential fungal pathogens in each group was2 %. Structural equation models revealed that oxygen concentration was a major factor influencing the bacterial diversity in CT, and the increase of oxygen concentration could limit methane emissions by inhibiting the growth of anaerobic bacteria. Therefore, membrane-covered composting could effectively improve compost safety and reduce methane emissions by regulating microbial community structure.

This study investigated the effects of micro-aerobic conditions on fungal community succession and dissolved organic matter transformation during dairy manure membrane-covered composting. The results showed that lignocellulose degradation in the micro-aerobic composting group (AC: oxygen concentration 5 %) was slower than that in the static composting group (SC: oxygen concentration 1 %), but the dissolved organic carbon in AC was greatly increased. The degree of aromatic polymerization was higher in AC than in SC. But the carboxyl carbon and alcohol/ether biodegradations were faster in SC than in AC, which promoted carbon dioxide and methane emissions, respectively. The relative abundances of pathogenic and dung saprotrophic fungi in AC were 44.6 % and 10.59 % lower than those in SC on day 30, respectively. Moreover, the relative abundance of soil saprotrophs increased by 5.18 % after micro-aerobic composting. Therefore, micro-aerobic conditions improved the quality of compost products by influencing fungal community evolution and dissolved organic matter transformation.

In a natural basin, the vegetation can change the slope convergence and affect the surface runoff. Vegetation height may vary in an area, showing a two-layer combination of high and low collocation. This study aimed to understand the effects of double-layer vegetation in different submerged states on flow resistance. Plantings of vegetation at different heights may control landslides and contribute to soil and water conservation. This study explored the water flow resistance characteristics of double-layer vegetation at different heights. A plastic bar was used to simulate rigid vegetation, and an indoor channel fixed bed experiment was used to simulate vegetation with different heights of 5 cm and 7 cm, 5 cm and 8 cm, 5 cm and 9 cm, and 5 cm and 10 cm. By analyzing the relationship between the Darcy–Weisbach resistance coefficient (f) and water depth (h), it was concluded that when the vegetation is in a non-submerged state, f and h satisfy f= 5.6427 h+ 0.0245. When the water depth just submerges the low vegetation, f changes abruptly, and f and h satisfy the relationship f= 3.4075 h + 0.0021. When the water depth is the same as the height of high vegetation, f attains the maximum value. In addition, the flow resistance f increases by 0.03 with a 1 cm increase in the vegetation height h. When the vegetation is completely submerged, f is negatively correlated with h.

Silicon is regarded as the next-generation alternative anode material of lithium–ion battery due to the highest theoretical specific capacity of 4200 mAh g−1. Nevertheless, the drastic volume expansion/shrink (~ 300%) during the lithiation/delithiation process and the poor electrical conductivity obstruct its commercial application. Herein, we report a novel design of litchi-structural Si@C nanoparticles (L-S Si@C) as long cycle life anode material for lithium–ion battery. The L-S Si@C with nanosized (~ 50 nm) SiC dispersed in the inner surface and ~ 6 wt% carbon layer covering the outer surface delivers a specific capacity of 1145 mAh g−1 in the initial cycle and maintains a specific capacity of 570 mAh g−1 after 300th cycling times, which can be attributed to the specially structurally stable L-S Si@C nanoparticles.

This study explored the effects of different particle sizes of rice straw biochar (RSB) on the methane emissions of pig manure/wheat straw aerobic composting experiments to provide a theoretical suggestion for biochar application. The experiments were conducted with a control group, powder (1 mm) group, and granular (4 mm-1 cm) group. Methane emissions increased by 56.84% in the powder group but decreased by 22.15% in the granular group during the aerobic composting. Methane was generated by methanogens and methanotrophs in the specific anaerobic micro-environment characterized by X-ray micro-computed tomography (micro-CT). The porosity of initial composting samples increased by 4.02% in the granular group but decreased by 3.88% in the powder group. RSB additives typically reduced the mcrA/pmoA ratio and increased the diversity of Bacteria and Archaea. Conclusively, granular biochar benefits to aerobic composting to alleviate the CH

Solid tumors always exhibit local hypoxia, resulting in the high metastasis and inertness to chemotherapy. Reconstruction of hypoxic tumor microenvironment (TME) is considered a potential therapy compared to directly killing tumor cells. However, the insufficient oxygen delivery to deep tumor and the confronting “Warburg effect” compromise the efficacy of hypoxia alleviation. Herein, we construct a cascade enzyme-powered nanomotor (NM-si), which can simultaneously provide sufficient oxygen in deep tumor and inhibit the aerobic glycolysis to potentiate anti-metastasis in chemotherapy. Catalase (Cat) and glucose oxidase (GOx) are co-adsorbed on our previously reported CAuNCs@HA to form self-propelled nanomotor (NM), with hexokinase-2 (HK-2) siRNA further condensed (NM-si). The persistent production of oxygen bubbles from the cascade enzymatic reaction propels NM-si to move forward autonomously and in a controllable direction along H2O2 gradient towards deep tumor, with hypoxia successfully alleviated in the meantime. The autonomous movement also facilitates NM-si with lysosome escaping for efficient HK-2 knockdown to inhibit glycolysis. In vivo results demonstrated a promising anti-metastasis effect of commercially available albumin-bound paclitaxel (PTX@HSA) after pre-treated with NM-si for TME reconstruction. This cascade enzyme-powered nanomotor provides a potential prospect in reversing the hypoxic TME and metabolic pathway for reinforced anti-metastasis of chemotherapy., Graphical abstract The cascade enzyme-powered nanomotor (NM-si) is constructed for tumor microenvironment modulation. Through persistently generating oxygen in deep tumor and inhibiting aerobic glycolysis, the lung metastasis of triple negative breast cancer is successfully inhibited.Image 1

Accurate detection of SF 6 decomposition characteristics components in SF 6 gas insulated equipment is one of the important technical method to realize fault diagnosis and condition monitoring of SF 6 gas insulated equipment. In this paper, based on the density functional theory, the infrared spectrum of SOF2 and SO 2 F 2 , that is two key SF 6 sulfur-containing decomposition characteristics components, were simulated and analyzed. The research found that the infrared spectrum of SOF2 molecule included four main absorption peaks at 485.106 cm−1, 678.479 cm−1, 748.357 cm−1, and 1295.070 cm−1, respectively. And the infrared spectrum of SO 2 F 2 molecule included five main absorption peaks at 501.852 cm−1, 779.442 cm−1, 814.284 cm−1, 1221.181 cm−1, and 1460.246 cm−1, respectively. This study lay a theoretical foundation for fault diagnosis and condition monitoring of SF 6 insulated equipment by mid infrared, and ensure the safety of power equipment.

SF 6 partial discharge decomposition characteristic component analysis method is one of the most efficient and convenient technology to diagnose latent defects in SF 6 gas insulated equipment. Compared with the traditional SF 6 characteristic decomposition gas detection method, the spectral method has many outstanding advantages, such as non-destructive detection of gas, high detection speed, high precision, and no need for mixed gas separation. However, the Raman characteristics of SF 6 partial discharge decomposition characteristic gas have not been fully clarified. Therefore, based on the density functional theory, the Raman spectra of SOF2 and SO 2 F 2 are calculated, which lays a solid theoretical foundation for SF 6 gas insulated equipment insulation fault diagnosis and condition monitoring method based on spectral technology.

The modification of targeting ligands on nanoparticles (NPs) is anticipated to enhance the delivery of therapeutics to diseased tissues. However, once exposed to the blood stream, NPs can immediately adsorb proteins to form the "protein corona," which may greatly hinder the targeting ligand from binding to its receptor. For brain-targeting delivery, nanotherapeutics must traverse the blood-brain barrier (BBB) to enter the brain parenchyma and then target the diseased cells. However, it remains elusive whether, apart from receptor recognition, the protein corona can affect other processes involved in BBB transcytosis, such as endocytosis, intracellular trafficking, and exocytosis. Furthermore, the targeting ability of NPs toward diseased cells after transcytosis remains unclear. Herein, transferrin (Tf), a brain-targeting ligand, was coupled to NPs to evaluate BBB transcytosis and brain tumor targeting ability. Different impacts of the in vitro and in vivo protein corona on receptor targeting, lysosomal escape, and BBB transcytosis were found. The in vitro protein corona abolished the Tf-mediated effects of the abovementioned processes, whereas the in vivo protein corona attenuated these effects. After crossing the BBB, Tf retained its targeting specificity towards brain tumor cells. Together, these results revealed that several bound apolipoproteins, especially apolipoprotein A-I, may help NPs traverse the BBB, thereby providing novel insights into the development of brain-targeted delivery.

The poor conductivity of olivine structure LiFePO4 led to inevitable hindered electrochemical performances and restricted their uses in Li-ion batteries. To overcome the problem, introduction of co...

Bacteria play an important role in organic matter degradation and maturity during aerobic composting. This study analyzed composting with or without a membrane cover in laboratory-scale aerobic composting reactor systems. 16S rRNA gene analysis was used to study the bacterial community succession during composting. The richness of the bacterial community decreased and the diversity increased after covering with a semi-permeable membrane and applying a slight positive pressure. Principal components analysis based on operational taxonomic units could distinguish the main composting phases. Linear Discriminant Analysis Effect Size analysis indicated that covering with a semi-permeable membrane reduced the relative abundance of anaerobic Clostridiales and pathogenic Pseudomonas and increased the abundance of Cellvibrionales. In membrane-covered aerobic composting systems, the relative abundance of some bacteria could be affected, especially anaerobic bacteria. Covering could effectively promote fermentation, reduce emissions and ensure organic fertilizer quality.

The commercial application of silicon anodes in Li-ion batteries has been seriously hindered by their poor cycling stability because of their huge volume changes during cycling. In this paper a self-healing ionomer binder, as a crosslinked lithiated polymer, is designed and synthesized for Si/graphite anodes with a low binder content of 1.8 wt%. The self-healing ionomer binder can form hydrogen bonds with Si and graphite particles, which can effectively control the swelling of the electrodes and retain the integrity of the electrodes upon cycling. In addition, Li+ in molecule chains can shorten the pathway to the surface of active materials. After cycling, the thickness of the electrodes using a self-healing ionomer binder increases by only about 30%. The self-healing ionomer binder enables enhanced cycling retention and can be used as a significant potential candidate for Si/graphite anodes with a commercial areal capacity of 3 mA h cm−2.

Silicon (Si) is considered as the most promising candidate for anode materials in the next-generation lithium-ion batteries (LIBs). Regulating the morphology and structure of Si plays a vital role in alleviating the volume expansion and improving electronic conductivity. Herein, an ingenious core-shell structure (denoted as CoMOF-D@Si@C) is synthesized by depositing Si uniformly on the pyrolytic metal-organic frameworks (MOFs) via chemical vapor deposition (CVD) method and then encapsulated with a carbon shell. The CoMOF-D@Si@C exhibits excellent rate capability and cycle performance, which delivers a high-rate capability of ~957 mAh g−1 at 10 A g−1 and a reversible capacity of 1493 mAh g−1 after 400 cycles. In particular, the capacity is maintained at 648 mAh g−1 after 1200 cycles at a high current density of 4 A g−1 with a rapid increase of the Coulombic efficiency (CE) to 99.8% after only 5 cycles and the average CE (99.7%) in the whole cycling at 4 A g−1. Profiting from the outer carbon shell, uniform Si deposition and inner porous pyrolytic MOF structure, this architecture can maintain structural stability and provide constructive conductivity during cycling processes. The superior electrochemical performance of the CoMOF-D@Si@C composite makes it a promising anode material for LIBs.

The effects of functional membrane covering (FMC) on nitrogen transformation and related functional genes during aerobic composting were investigated by performing a comparable experiment. The FMC increased the pile temperature, promoted compost maturity, and decreased nitrogen loss. The FMC reduced NH3 and N2O emissions by 7.34% and 26.27%, respectively. The water film and the micro-positive pressure environment under the membrane effectively prevented NH3 escaping. The FMC up-regulated the amoA gene copy number (promoting NH3/NH4+ oxidation). The reduction of N2O emission by the FMC was mainly related to denitrifying genes (nirK, nirS, and nosZ). The FMC down-regulated the nirK and nirS gene copy numbers, but up-regulated the nosZ gene copy number. Pearson correlation analysis indicated that the functional membrane characteristics and differences between the composting pile environments caused by the FMC significantly affected the nitrogen forms and the related functional genes. The FMC strongly decreased nitrogen emissions and therefore conserved nitrogen.

Silicon is regarded as the next generation anode material for lithium-ion batteries because of its high specific capacity, low intercalation potential and abundant reserves. However, huge volume changes during the lithiation and delithiation processes and low electrical conductivity obstruct the practical applications of silicon anodes. In this study, a treble-shelled porous silicon (TS-P-Si) structure was synthesized via a three-step approach. The TS-P-Si anode delivered a capacity of 858.94 mA h g−1 and a capacity retention of 87.8% (753.99 mA h g−1) after being subjected to 400 cycles at a current density of 400 mA g−1. The good cycling performance was due to the unique structure of the inner silicon oxide layer, middle silver nano-particle layer and outer carbon layer, leading to a good conductivity and a decreased volume change of this silicon-based anode.

To investigate the emission mechanism of ammonia (NH3) and nitrous oxide (N2O) during aerobic composting and the influence of powder bamboo biochar (PBB) on this process, this paper conducted a systematic study on the nitrogen-transforming functional microbial community, including functional genes, microbial structure and metabolism pathways. PBB reduced N2O and NH3 emissions by 1.25%-8.72% and 10.4%-11.8%, respectively. The quantitative PCR results indicated that the reduced N2O emission by PBB were mainly related to denitrifying genes (nirS, nirK, nosZ, and narG). The metagenome results demonstrated that Nitrosococcus was the main genus that could oxidize ammonia to nitrite decreased by PBB. The PBB significantly affected the nitrogen metabolism pathway, reduced the activity of glutamate dehydrogenase to inhibit the formation of NH4+ to reduce NH3 emission. The higher N2O emission in the control group was also related to the higher relative contents of hydroxylamine reductase and nitrite reductase.

Biomechanical behavior is a fundamental property for the efficient utilization of wheat straw in such applications as fuel and renewable materials. Tensile experiments and lignocellulose analyses were performed on three types of wheat straw. A multi-scale finite element model composed of the microscopic model of the microfibril equivalent volume element and the macroscopic model of straw tissue was proposed based on the physiological structure and lignocellulose components of wheat straw. The tensile properties of wheat straw were simulated by ANSYS software. The predicted stress-strain data were compared with the observed data, and good correspondence was achieved for all three types of wheat straw. The validated multi-scale finite-element (FE) model was then used to investigate the effect of the lignocellulose components on the biomechanical properties of wheat straw. More than 80% of stress is carried by the cellulose fiber, whereas the strain is mainly carried by the amorphous cellulose.

Greenhouse gas and ammonia emissions during composting with different biochar types and particle sizes were investigated. Compared with powder-biochar, granular-biochar improved pore connectivity and was benefit to methanotrophs activities, like Methylococcaceae, reducing CH4 emissions. At the same particle size, bamboo biochar (BB) had a higher pore volume and more aerobic microenvironment within the compost than rice straw biochar (RSB), reducing GHG emissions. Bamboo biochar had high aromatic compound and NO3- concentrations and therefore surface π-π electron donor/acceptor interactions, causing low N2O emissions and inhibiting denitrifying bacteria (e.g., Bacteroidales). More CO and CO bonds in rice straw biochar than bamboo biochar caused lower NH3 emissions using rice straw than bamboo biochar. Powdered biochar had more exposed reactive functional groups and decreased NH3 production better than granular biochar. Powdered bamboo biochar controls gaseous emissions better than other biochars during aerobic pig manure/wheat straw composting.