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Aims Rotation is an effective strategy for controlling crop diseases and improving plant health. However, the effect of a mushroom-tobacco rotation on the composition and structure of microbial communities in continuous cropping soil is unclear. Methods and results This study analysed the structure and function of soil bacterial and fungal communities using Illumina MiSeq high-throughput 16S rRNA gene sequencing. The results showed that the physicochemical properties (organic matter, available nitrogen, available phosphorus, and available potassium) and enzymatic activity (phosphatase, catalase, urease and invertase activity) in the rotation treatments (Y1, M1, Y2 and M2) were significantly higher than those in the control (continuous cropping) treatment (CK) and reached peak values in the M2 treatment. PCA showed that the soil microbial community structure in each rotation treatment was different from that in the control. The dominant bacterial phyla of the different soil treatments were Proteobacteria and Actinobacteriota, and the dominant fungal phyla of the different soil treatments were Ascomycota and Basidiomycota. The M2 rotation significantly reduced the relative abundance of harmful fungi (Penicillium and Gibberella) compared to the other treatments. RDA showed that the most abundant bacterial taxa were negatively correlated with pH and positively correlated with physicochemical properties. However, the most abundant fungal taxa were positively correlated with pH and negatively correlated with physicochemical properties. Conclusions The mushroom-tobacco rotation can effectively maintain the ecological balance of the substrate microbial environment, and provide a more effective way to prevent the continuous cropping of tobacco.

The main component is the manufacturing process of actual tobacco crushing and the redrying procedure. As a result, it requires a high level of overall stability. People should make sure the maintenance and repair system is appropriately considered as a significant task in redrying. Various flavors, including black and green, were tested for tobacco consumption. According to the researchers who tested for tobacco consumption, the amount of nicotine in tea leaves is proved to be highly varying in some cases, much higher than that found in Solanaceae fruits. Here, describing inexpensive redrying purification for homogenization process (IRPHP), with this technique, colicins can be recovered with a higher detection rate and nicotine levels can be reduced that can be used on food. After [Formula: see text]-means cluster verification, one nicotine leaf may be precisely split into several zones for redrying the distribution of exhaust tobacco aroma components (TACs) in various global locations. In general, these findings give essential information to aid in the efficient harvesting and redrying of tobacco plants. Additionally, these data imply that this approach may be used to determine the chemical distributions in leaves that do not have the same arrangement as tobacco leaves.