13 results on '"Xuanshu He"'
Search Results
2. A Novel Protein Sourced from Chinese Medicine Residue for Golden Pompano Feed: Endothelium Corneum Gigeriae Galli Residue (ECGGR)
- Author
-
Ziqiao Wang, Rong Yao, Xuanshu He, Xin Cui, Zhihong Liao, Yantao Liu, Hanlin Wei, Zhenxiao Zhuang, Mengdie Chen, and Jin Niu
- Subjects
Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Fishmeal is an important protein source in aquafeed. However, due to the limited natural resources, fishmeal is in short supply, resulting in a price surge for fishmeal. Here, we reported a kind of Chinese medicine residue, endothelium corneum gigeriae galli residue (ECGGR), as a fishmeal substitute in the diets of Trachinotus ovatus. Six isonitrogenous and isolipidic diets were formulated, substituting fishmeal at 0%, 6.25%, 12.5%, 18.75%, 25%, and 31.25%. There was no significant difference in the growth performance when the fishmeal substitution level was no more than 25%. The smallest FCR was obtained at the 18.75% substitution level. Furthermore, substituting ECGGR for fishmeal had no effect on whole-body and muscle proximate compositions, except when the replacement level exceeded 25%, which led to a decrease in whole-body moisture and an increase in whole-body crude protein. The contents of Gly, Cys, Ile, Tyr, Pro, and EAAs/TAAs were altered as the substitution level varied. However, dietary replacement of fishmeal with ECGGR did not degrade muscle protein quality, according to a nutritional evaluation of muscle essential amino acid composition. In terms of hepatic antioxidant capacity, neither the overall antioxidant status nor the expression of genes in the Nrf2-ARE pathway was altered by dietary ECGGR. Moreover, the expressions of p65, TNF-α, and IL-8 in the intestine were upregulated at the 31.25% substitution level. Also, more goblet cells were observed in the intestine at substitution levels of 25% and 31.25%. In conclusion, ECGGR can substitute for fishmeal at the optimal level of 18.75% without adversely affecting the growth performance, protein quality, or hepatic and intestinal health of golden pompano.
- Published
- 2024
- Full Text
- View/download PDF
3. Dietary Supplementation of Astragalus membranaceus Extract Affects Growth Performance, Antioxidant Capacity, Immune Response, and Energy Metabolism of Largemouth Bass (Micropterus salmoides)
- Author
-
Xuanshu He, Anqi Chen, Zhihong Liao, Jian Zhong, Anda Cheng, Xinghua Xue, Fuyuan Li, Mengdie Chen, Rong Yao, Wei Zhao, and Jin Niu
- Subjects
Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
The present study investigated the effects of Astragalus membranaceus extract (AME) on growth performance, immune response, and energy metabolism of juvenile largemouth bass (Micropterus salmoides). Seven diets containing 0%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, and 0.6% AME (Con, AME0.1, AME0.2, AME0.3, AME0.4, AME0.5, and AME0.6 groups) were formulated and fed to M. salmoides for 8 weeks. Final body weight (FBW), feed intake (FI), weight gain (WG), and specific growth rate (SGR) were all significantly higher in AME0.4 group than in Con group (P
- Published
- 2024
- Full Text
- View/download PDF
4. Effects of Dietary Rhodobacter sphaeroides Protein Substitution of Fishmeal and Coenzyme Q10 Supplementation on Growth Performance, Intestinal Microbiota and Stress Tolerance of Litopenaeus vannamei in Acute Low Salinity
- Author
-
Zhihong Liao, Yangyang Gong, Ziqiao Wang, Yingjie Wang, Rong Yao, Mengdie Chen, Dan Wei, Wei Zhao, Xuanshu He, and Jin Niu
- Subjects
Litopenaeus vannamei ,nutrition ,Rhodobacter sphaeroides protein ,CoQ10 ,intestinal microbiota ,low salt stress ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
A 56-day culture experiment followed by an acute low salinity challenge was conducted to evaluate the effects of Rhodobacter sphaeroides protein (RSP) substitution of fishmeal and Coenzyme Q10 (CoQ10) supplementation on growth performance, intestinal microbiota and compressive capacity of Litopenaeus vannamei (L. vannamei). Four experimental diets were formulated: FM diet (20% fishmeal), RSP diet (20% RSP instead of 20% fishmeal), CoQ10 diet (20% fishmeal supplemented with CoQ10 at 0.08 g kg–1), RSP + CoQ10 diet (20% RSP supplemented with CoQ10 at 0.08 g kg–1). The obtained results were denoted that L. vannamei fed CoQ10 diet could improve growth performance (weigh gain and specific growth rate), condition factor and crude lipid, and decrease hepatosomatic index, but no differences were found in survival rates. High throughput sequencing on intestinal microbiota indicated that intestinal microbiota of L. vannamei consisted mainly of Proteobacteria and Firmicutes. The species richness of the RSP diet was remarkably higher than that of the other diets. Moreover, the presence of RSP and CoQ10 may improve intestinal homeostasis by inhibiting the propagation of Vibrio. Compared to FM diet and RSP diet, supplementation of CoQ10 significantly improved the compressive capacity of L. vannamei against the acute low salinity challenge, as indicated by higher survival rates as well as higher activities of T-AOC and higher transcript levels of SOD, HSP70, and Relish gene. Our findings demonstrated that RSP could serve as a novel FM and CoQ10 could serve as a prospective feed additive to help L. vannamei to overcome environmental stresses.
- Published
- 2022
- Full Text
- View/download PDF
5. Replacement of Astaxanthin With Lutein in Diets of Juvenile Litopenaeus vannamei: Effects on Growth Performance, Antioxidant Capacity, and Immune Response
- Author
-
HaoHang Fang, XuanShu He, HuLong Zeng, YongJian Liu, LiXia Tian, and Jin Niu
- Subjects
lutein ,L. vannamei ,astaxanthin ,growth performance ,antioxidant capacity ,immunity ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
An 8-week feeding experiment was conducted to investigate whether diet supplementation of lutein could result in similar growth performance, antioxidant capacity, and immunity of Litopenaeus vannamei when compared to dietary astaxanthin. Juvenile L. vannamei (initial body weight: 0.64 ± 0.04 g) were fed with one of five isonitrogen and isolipids diets with/without lutein or astaxanthin [control group (C); the lutein (L) groups contained 0, 62.5, 75, 87.5 ppm lutein, respectively, the astaxanthin (A) group contained 50 ppm astaxanthin]. Results showed that dietary supplementation of lutein ranging from 62.5 to 75 ppm resulted in similar growth performance (WGR, SGR, FCR, and SR) of L. vannamei compared with the A group (P > 0.05). Apart from that, no statistical difference was observed in antioxidant parameters (hemolymph T-AOC, hemolymph MDA, and RNA expression level of GSH-PX, CAT), anti-inflammatory ability (Relish, Rho, and HSP70) and apoptosis-related gene expression (Caspase3) among lutein treatments ranging from 62.5 to 87.5 ppm and the A group (P > 0.05). These results indicate that a dose of 62.5–75 ppm of lutein was suitable in the diet of L. vannamei for substituting dietary astaxanthin.
- Published
- 2021
- Full Text
- View/download PDF
6. Cloning and expression analysis of Keap1 of golden pompano (Trachinotus ovatus) and response to oxidized fish oil and LPS administration
- Author
-
Jiajun Xie, Xu Chen, Xuanshu He, and Jin Niu
- Subjects
Trachinotus ovatus ,Nrf2-Keap1 pathway ,Keap1 ,Oxidazed fish oil ,LPS administration ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
Keap1 is an important component of Nrf2-Keap1 signaling pathways. However, information about Keap1 in T. ovatus is quiet limit. Thus, the full length of Keap1 was cloned from T. ovatus in this study. The full-length cDNA sequence was 3373 bp in length and comprised of a 1878 bp ORF preceded by 1205bp 5′-UTR, and followed a 290 bp 3′- UTR. The putative amino acid sequence of the Keap1 contains 625 residues. The Keap1 amino acid sequence contained a BTB domain, an IVR domain and a DGR domain. The phylogenetic tree showed that Seriola dumerili had the highest identity with T. ovatus in Keap1 amino acid. The results show that Keap1 gene was predominantly expressed in liver, kidney, spleen and gill, significantly higher than that of other tissues. The relative expression of Keap1 in foregut and heart were moderately expressed. After feeding with oxidized fish oil for 8 weeks, the head kidney expression levels of Keap1, Nrf2, HO-1, SOD and CAT in T. ovatus were significantly higher than those in control group. Administration of LPS for 72 h in T. ovatus activated the Nrf2-Keap1 pathways and upregulated the expression level of HO-1, GSH-PX, SOD and CAT. In conclusion, Keap1 is evolutionarily and functionally conserved in T. ovatus. Keap1 expression level was significantly higher in oxidative stress caused by oxidized fish oil and LPS administration, suggesting that Keap1 plays a pivotal role in Nrf2-Keap1 pathway of T. ovatus.
- Published
- 2020
- Full Text
- View/download PDF
7. Diet supplementation of organic zinc positively affects growth, antioxidant capacity, immune response and lipid metabolism in juvenile largemouth bass, Micropterus salmoides
- Author
-
Xuanshu He, Anqi Chen, Zhihong Liao, Yufan Zhang, Gang Lin, Zhenxiao Zhuang, Yantao Liu, Hanlin Wei, Ziqiao Wang, Yingjie Wang, and Jin Niu
- Subjects
Nutrition and Dietetics ,Medicine (miscellaneous) - Abstract
Zn is an important trace element involved in various biochemical processes in aquatic species. An 8-week rearing trial was thus conducted to investigate the effects of Zn on juvenile largemouth bass (Micropterus salmoides) by feeding seven diets, respectively, supplemented with no Zn (Con), 60 and 120 mg/kg inorganic Zn (Sul60 and Sul120), and 30, 60, 90 and 120 mg/kg organic Zn (Bio30, Bio60, Bio90 and Bio120). Sul120 and Bio120 groups showed significantly higher weight gain and specific growth rate than Con group, with Bio60 group obtaining the lowest viscerosomatic index and hepatosomatic index. 60 or 90 mg/kg organic Zn significantly facilitated whole body Zn retention. Up-regulation of hepatic superoxide dismutase, glutathione peroxidase and catalase activities and decline of malondialdehyde contents indicated augmented antioxidant capacities by organic Zn. Zn treatment also lowered plasma aminotransferase levels while promoting acid phosphatase activity and hepatic transcription levels of alp1, acp1 and lyz-c than deprivation of Zn. The alterations in whole body and liver crude lipid and plasma TAG contents illustrated the regulatory effect of Zn on lipid metabolism, which could be possibly attributed to the changes in hepatic expressions of acc1, pparγ, atgl and cpt1. These findings demonstrated the capabilities of Zn in potentiating growth and morphological performance, antioxidant capacity, immunity as well as regulating lipid metabolism in M. salmoides. Organic Zn could perform comparable effects at same or lower supplementation levels than inorganic Zn, suggesting its higher efficiency. 60 mg/kg supplementation of organic Zn could effectively cover the requirements of M. salmoides.
- Published
- 2023
8. Effects of Dietary Supplementation of B. Subtilis on the Growth, Immune Responses and Acute Ammonia Stress Tolerance of Nile Tilapia (Oreochromis Niloticus)
- Author
-
Zhihong Liao, Hanlin Wei, Xuanshu He, Ziqiao Wang, Yantao Liu, Zhenxiao Zhuang, Wei Zhao, Juyun He, and jin niu
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
9. Dual-Function Analysis of Astaxanthin on Golden Pompano (Trachinotus ovatus) and Its Role in the Regulation of Gastrointestinal Immunity and Retinal Mitochondrial Dysfunction Under Hypoxia Conditions
- Author
-
Xuanshu He, Jia-Jun Xie, Dan-Qi Lu, Jin Niu, Hao-Hang Fang, Wei Zhao, and Shiyu Liao
- Subjects
Fish mortality ,retina ,Physiology ,Biology ,medicine.disease_cause ,lcsh:Physiology ,chemistry.chemical_compound ,Astaxanthin ,Physiology (medical) ,Lactobacillus ,medicine ,Trachinotus ovatus ,Food science ,Original Research ,gut morphology and microbiota ,lcsh:QP1-981 ,TFAM ,biology.organism_classification ,mitochondrial ,astaxanthin ,chemistry ,Pompano ,Lysozyme ,eyeball morphology ,Oxidative stress - Abstract
The present study investigated the potential mechanisms of astaxanthin in the regulation of gastrointestinal immunity and retinal mitochondrial function of golden pompano (Trachinotus ovatus). Triplicate groups of juvenile T. ovatus (mean initial weight: 6.03 ± 0.01 g) were fed one of six diets (D1, D2, D3, D4, D5, and D6) for 8 weeks, with each diet containing various concentrations of astaxanthin (0, 0.0005, 0.001, 0.005, 0.01, or 0.1%, respectively). Growth performance of fish fed the D2–D5 diets was higher than that of fish fed the D1 diet; however, growth performance and survival of fish deteriorated sharply in fish fed the D6 diet. Gut villus in fish fed the D2–D5 diets were significantly longer and wider than that of fish fed the D6 diet. Feeding with D2–D5 diets led to increased abundance of Bacillus, Pseudomonas, Oceanobacillus, Lactococcus, Halomonas, Lactobacillus, and Psychrobacter while abundance of Vibrio and Bacterium decreased. Additionally, feeding with the D6 diet resulted in a sharp decline in Pseudomonas and Lactobacillus abundance and a sharp increase in Vibrio abundance. A low dissolved oxygen environment (DO, 1.08 mg/L) was conducted for 10 h after the rearing trial. No fish mortality was observed for any of the diet treatments. Lysozyme (LZY) activity in fish fed the D6 diet decreased sharply and was significantly lower than that in other groups. ROS production also decreased sharply in fish fed the D6 diet. Moreover, the conjunctiva and sclera in the fish fed the D6 diet were indistinguishable. Suitable dietary astaxanthin supplementation levels (0.005–0.1%) exerting a neuroprotective effect from low dissolved oxygen environments is due to up-regulated expression of anti-apoptotic factors, such as phosphorylated Bcl-2-associated death promoter (pBAD), phosphorylated glycogen synthase kinase-3β (pGSK-3β), Bcl-2 extra large (Bcl-xL), and down-regulated expression of Bcl-2-associated X protein (Bax) pro-apoptotic factor in retinas. Furthermore, suitable dietary astaxanthin levels (0.0005–0.01%) suppressed up-regulation of critical mitochondrial components, such as peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), mitochondrial transcription factor A (TFAM), and mitochondrial DNA (mtDNA), while excessive astaxanthin supplementation produces the opposite effect. In brief, high-dose astaxanthin arouses and aggravates low dissolved oxygen-induced inflammation, oxidative stress, intestinal disorder, retinal apoptosis, and retinal mitochondrial dysfunction in T. ovatus. Second-degree polynomial regression of WG indicated that the optimum dietary astaxanthin for juvenile T. ovatus is 0.049%.
- Published
- 2020
10. Comparison effect of Rhodobacter sphaeroides protein replace fishmeal on growth performance, intestinal morphology, hepatic antioxidant capacity and immune gene expression of Litopenaeus vannamei under low salt stress
- Author
-
Dan Wei, Zhihong Liao, Wei Zhao, Xuanshu He, Yangyang Gong, and Jin Niu
- Subjects
animal structures ,Antioxidant ,biology ,medicine.medical_treatment ,fungi ,Litopenaeus ,Aquatic Science ,Intestinal morphology ,biology.organism_classification ,Hsp70 ,Shrimp ,Rhodobacter sphaeroides ,Fish meal ,Low salt ,medicine ,Food science - Abstract
The present study was conducted to evaluate the effect of replacing fishmeal (FM) with Rhodobacter sphaeroides protein on growth performance, intestinal morphology and antioxidant capability of Pacific white shrimp (Litopenaeus vannamei) in an eight-week feeding experiment. FM protein of a FM-based control diet (FM20) was replaced with 0 (FM20), 4 (FM16), 8 (FM12), 12 (FM8), 16 (FM4), and 20% (FM0) by Rhodobacter sphaeroides protein to formulate six experimental diets. Compared with control group, the shrimp fed 4% (FM16) and 8% (FM12) Rhodobacter sphaeroides protein gained higher survival rate and specific growth rate (SGR). There were no significant differences in MDA content of hepatopancreases and crude protein in whole body, while crude lipid was increased significantly in shrimp fed with Rhodobacter sphaeroides protein. In addition, there was no negative impact on the intestinal morphology of shrimp by FM replacement with Rhodobacter sphaeroides protein. The challenge test with low salt stress showed that the survival rate of shrimp fed with Rhodobacter sphaeroides protein were not significantly different from that of shrimp fed with whole fishmeal. Furthermore, higher T-AOC and GSH-PX activities of hepatopancreases of shrimp were observed in Rhodobacter sphaeroides protein after low salt stress. Moreover, compared with the control group, substitution of Rhodobacter sphaeroides significantly improved the resistance of L. vannamei against the stress of acute low salinity, as indicated by higher expression levels of SOD, Relish and HSP70 and lower expression levels of Caspase-3. Our findings suggested that Rhodobacter sphaeroides protein, has improved growth performance and maintained the intestinal tract healthy, and enhanced antioxidant competence and immunity of L. vannamei and could serve as novel FM replacement in shrimp feed that helps shrimp to overcome low salt challenge.
- Published
- 2022
11. Identification of heme oxygenase-1 from golden pompano (Trachinotus ovatus) and response of Nrf2/HO-1 signaling pathway to copper-induced oxidative stress
- Author
-
Yong-Jian Liu, Shiyu Liao, Xuanshu He, Jin Niu, Jia-Jun Xie, Hao-Hang Fang, and Li-Xia Tian
- Subjects
Environmental Engineering ,NF-E2-Related Factor 2 ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,02 engineering and technology ,Oxidative phosphorylation ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Antioxidants ,Superoxide dismutase ,chemistry.chemical_compound ,medicine ,Environmental Chemistry ,Animals ,Tissue Distribution ,Heme ,Trachinotus ovatus ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,biology ,Glutathione peroxidase ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Sequence Analysis, DNA ,biology.organism_classification ,Pollution ,Molecular biology ,020801 environmental engineering ,Perciformes ,Heme oxygenase ,Oxidative Stress ,chemistry ,Pompano ,biology.protein ,Oxidoreductases ,Oxidative stress ,Copper ,Heme Oxygenase-1 ,Signal Transduction - Abstract
Heme oxygenase-1(HO-1) is a stress-inducible enzyme that mediates antioxidative and cytoprotective effects to maintain cellular redox homeostasis. In the present study, the full sequence of HO-1 was cloned from golden pompano(Trachinotus ovatus) by RT-PCR and RACE-PCR. The full cDNA sequence of HO-1 was 1349 bp in length which comprised of a 726 bp open reading frame (ORF) preceded by 262 bp 5′-untranslated region (UTR), and followed by a 360 bp 3′UTR, encoding 241 amino acid residues. Phylogenetic analysis revealed that HO-1 showed highest similarity to that of Takifugu rubripes. Tissue distribution analysis showed that the expression level of HO-1 was relatively high in heart, liver and spleen. A trial was conducted to investigate the response of Nrf2/HO-1 signaling pathway to oxidative stress induced by copper. The results showed that mRNA expression of NF-E2-related nuclear factor2 (Nrf2), Kelch-like-ECH-associated protein1 (keap1), superoxide dismutase (SOD), catalase (CAT), HO-1, NAD(P)H quinone oxidoreductase 1 (NQO1) and Glutathione peroxidase (GSH-PX) all significantly increased in copper treated group than that in the control group. This work provides new insight into the molecular mechanism underlying the Nrf2/HO-1 pathway in oxidative response in T. ovatus.
- Published
- 2019
12. Dietary supplementation of bile acid attenuate adverse effects of high-fat diet on growth performance, antioxidant ability, lipid accumulation and intestinal health in juvenile largemouth bass (Micropterus salmoides)
- Author
-
Peng Yin, Jin Niu, Yong-Jian Liu, Zhenxiao Zhuang, Xipei Tang, Shiwei Xie, Li-Xia Tian, and Xuanshu He
- Subjects
Antioxidant ,food.ingredient ,medicine.drug_class ,medicine.medical_treatment ,Micropterus ,Aquatic Science ,03 medical and health sciences ,Bass (fish) ,chemistry.chemical_compound ,food ,Animal science ,Chenodeoxycholic acid ,medicine ,Juvenile ,030304 developmental biology ,0303 health sciences ,Bile acid ,biology ,Lipid metabolism ,04 agricultural and veterinary sciences ,biology.organism_classification ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,medicine.symptom ,Weight gain - Abstract
Bile acid (BA) has been reported to improve growth performance and play an important role in lipid metabolism of fish. Five diets were formulated to investigate the effects of dietary bile acid (chenodeoxycholic acid) supplementation on growth performance, antioxidant capacity, lipid metabolism and intestinal health of juvenile largemouth bass (Micropterus salmoides). Fish (18.35 ± 0.05 g) were randomly fed five diets: the positive control diet (10.87% lipid, C), high fat diet (18.08% lipid, HF), and HF diets supplemented with 300, 600 and 900 mg/kg chenodeoxycholic acid (HFC1, HFC2 and HFC3, respectively). After 9 weeks of feeding experiment, the weight gain (WG) and special growth rate (SGR) were significantly lower in the fish fed diet HF compared with those fed diet C (P 0.05). Viscerosomatic index (VSI) and intraperitoneal fat ratio (IPF) were significantly higher in fish fed high fat diets than those fed the control diet (P 0.05), while the crude lipid of muscle significantly decreased in HFC2 group (P
- Published
- 2021
13. Molecular cloning, functional characterization and expression analysis of p65 subunit of golden pompano (Trachinotus ovatus) and response to high fat diet and LPS administration
- Author
-
Zhenxiao Zhuang, Dan Wei, Jin Niu, Shiyu Liao, Hao-Hang Fang, Jia-Jun Xie, Yong-Jian Liu, Li-Xia Tian, Xuanshu He, and Ruihua Wang
- Subjects
chemistry.chemical_classification ,Untranslated region ,0303 health sciences ,biology ,Protein subunit ,04 agricultural and veterinary sciences ,Aquatic Science ,Molecular cloning ,biology.organism_classification ,Molecular biology ,Amino acid ,03 medical and health sciences ,Open reading frame ,chemistry ,Complementary DNA ,Pompano ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Trachinotus ovatus ,030304 developmental biology - Abstract
p65 is an important component of NF-κB signaling pathways. However, information about p65 subunit in golden pompano (Trachinotus ovatus) is quiet limit. Thus, the full length of p65 subunit was cloned from T. ovatus in this study. The full-length cDNA sequence was 3162 bp in length and comprised of a 1886 bp open reading frame (ORF) preceded by 153 bp 5′-untranslated region (UTR), and followed by a 1143 bp 3′UTR. The p65 of T. ovatus contains a conserved N-terminal REL-homology domain (RHD) with 273 amino acids. The phylogenetic tree showed that p65 in T. ovatus was clustered in the clade of that in marine fish. mRNA expression of p65 was detected in 12 different tissues. The results showed that expression of p65 in liver was higher than that in other tissues (P 0.05). After 8-weeks feeding, the hepatic expression level of p65 and IL1β in fish fed diet with high fat showed significant difference with that in control diet (P
- Published
- 2020
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.