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3. Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant (pind) in eggplant (Solanum melongena L.)

Author

Luo, Lei, primary, Molthoff, Jos, additional, Li, Qiang, additional, Liu, Ying, additional, Luo, Shuangxia, additional, Li, Na, additional, Xuan, Shuxin, additional, Wang, Yanhua, additional, Shen, Shuxing, additional, Bovy, Arnaud G., additional, Zhao, Jianjun, additional, and Chen, Xueping, additional

Published
2023
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9. Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant (pind) in eggplant (Solanum melongena L.)

Author

Luo, Lei, Molthoff, Jos, Li, Qiang, Liu, Ying, Luo, Shuangxia, Li, Na, Xuan, Shuxin, Wang, Yanhua, Shen, Shuxing, Bovy, Arnaud G., Zhao, Jianjun, Chen, Xueping, Luo, Lei, Molthoff, Jos, Li, Qiang, Liu, Ying, Luo, Shuangxia, Li, Na, Xuan, Shuxin, Wang, Yanhua, Shen, Shuxing, Bovy, Arnaud G., Zhao, Jianjun, and Chen, Xueping

Abstract

Eggplant (Solanum melongena L.) is a highly nutritious and economically important vegetable crop. However, the fruit peel of eggplant often shows poor coloration owing to low-light intensity during cultivation, especially in the winter. The less-photosensitive varieties produce anthocyanin in low light or even dark conditions, making them valuable breeding materials. Nevertheless, genes responsible for anthocyanin biosynthesis in less-photosensitive eggplant varieties are not characterized. In this study, an EMS mutant, named purple in the dark (pind), was used to identify the key genes responsible for less-photosensitive coloration. Under natural conditions, the peel color and anthocyanin content in pind fruits were similar to that of wildtype ‘14-345’. The bagged pind fruits were light purple, whereas those of ‘14-345’ were white; and the anthocyanin content in the pind fruit peel was significantly higher than that in ‘14-345’. Genetic analysis revealed that the less-photosensitive trait was controlled by a single dominant gene. The candidate gene was mapped on chromosome 10 in the region 7.72 Mb to 11.71 Mb. Thirty-five differentially expressed genes, including 12 structural genes, such as CHS, CHI, F3H, DFR, ANS, and UFGT, and three transcription factors MYB113, GL3, and TTG2, were identified in pind using RNA-seq. Four candidate genes EGP21875 (myb domain protein 113), EGP21950 (unknown protein), EGP21953 (CAAX amino-terminal protease family protein), and EGP21961 (CAAX amino-terminal protease family protein) were identified as putative genes associated with less-photosensitive anthocyanin biosynthesis in pind. These findings may clarify the molecular mechanisms underlying less-photosensitive anthocyanin biosynthesis in eggplant.

Published
2023

10. Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’

Author

Li, Hui, primary, Gu, Aixia, additional, Feng, Daling, additional, Li, Na, additional, Yang, Rui, additional, Zhang, Xinpei, additional, Luo, Shuangxia, additional, Karamat, Umer, additional, Wang, Qianyun, additional, Xuan, Shuxin, additional, Chen, Xueping, additional, Lu, Yin, additional, Wang, Yanhua, additional, Shen, Shuxing, additional, and Zhao, Jianjun, additional

Published
2023
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13. Mutation in a chlorophyll-binding motif of Brassica ferrochelatase enhances both heme and chlorophyll biosynthesis

Author

Liu, Mengyang, primary, Ma, Wei, additional, Su, Xiangjie, additional, Zhang, Xiaomeng, additional, Lu, Yin, additional, Zhang, Shaowei, additional, Yan, Jinghui, additional, Feng, Daling, additional, Ma, Lisong, additional, Taylor, Aoife, additional, Ge, Yunjia, additional, Cheng, Qi, additional, Xu, Kedong, additional, Wang, Yanhua, additional, Li, Na, additional, Gu, Aixia, additional, Zhang, Ju, additional, Luo, Shuangxia, additional, Xuan, Shuxin, additional, Chen, Xueping, additional, Scrutton, Nigel S., additional, Li, Chengwei, additional, Zhao, Jianjun, additional, and Shen, Shuxing, additional

Published
2022
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14. Glucosinolates in Self-crossed Progenies of Monosomic Cabbage Alien Addition Lines in Chinese Cabbage

Author

Gu Aixia, Zhao Jianjun, Wang Yanhua, Li Xiaofeng, Xuan Shuxin, and Shen Shuxing

Subjects
Chinese cabbage, cabbage, glucosinolate, monosomic alien addition lines, Plant culture, SB1-1110
Abstract

Brassica species have been reported to possess cancer preventive activity due to glucosinolates (GLS) and their derived properties. Many studies on GLS have focused on Brassica oleracea and Brassica rapa. However, information on GLS in progeny between Chinese cabbage (B. rapa ssp. pekinensis) and cabbage (B. oleracea var. capitata) remains limited. In this study, eight GLS were detected in the self-crossed progenies of monosomic cabbage alien addition lines in Chinese cabbage (Chinese cabbage – cabbage MAALs) and parental Chinese cabbage, and nine GLS were detected in the parental cabbage. The variation of GLS content ranges was greater in the progeny than in the parental Chinese cabbage. The nine GLS identified were subjected to PCA to evaluate the differences among progeny and parents. Eight progeny samples had a comprehensive principal component score closer to or greater than that of cabbage, and four of them exhibited glucoraphanin (GRA) and total GLS contents greater than that of Chinese cabbage with the relative content of total indolic GLS was greater than 50%. These results offered new opportunity to improve GLS-containing of Chinese cabbage using genes from cabbage.

Published
2015
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15. Genome-Wide Identification, Characterization, and Transcriptomic Analysis of the Cyclin Gene Family in Brassica rapa

Author

Zulfiqar, Sumer, primary, Zhao, Tiantian, additional, Liu, Yuanming, additional, Wei, Lai, additional, Farooq, Muhammad Awais, additional, Tabusam, Javaria, additional, Zhao, Jianjun, additional, Chen, Xueping, additional, Wang, Yanhua, additional, Xuan, Shuxin, additional, Li, Na, additional, Lu, Yin, additional, Luo, Shuangxia, additional, Shen, Shuxing, additional, and Gu, Aixia, additional

Published
2022
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16. Genome-Wide Transcriptome Analysis Reveals That Upregulated Expression of Aux/IAA Genes Is Associated with Defective Leaf Growth of the slf Mutant in Eggplant

Author

Du, Wenchao, primary, Lu, Yang, additional, Luo, Shuangxia, additional, Yu, Ping, additional, Shen, Jiajia, additional, Wang, Xing, additional, Xuan, Shuxin, additional, Wang, Yanhua, additional, Zhao, Jianjun, additional, Li, Na, additional, Chen, Xueping, additional, and Shen, Shuxing, additional

Published
2022
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18. OCTOPUS regulates BIN2 to control leaf curvature in Chinese cabbage

Author

Zhang, Xiaomeng, primary, Ma, Wei, additional, Liu, Mengyang, additional, Li, Xing, additional, Li, Jingrui, additional, Lu, Yin, additional, Li, Guanghuan, additional, Zhang, Shu, additional, Feng, Daling, additional, Wang, Yanhua, additional, Liang, Hao, additional, Luo, Shuangxia, additional, Li, Na, additional, Gu, Aixia, additional, Xuan, Shuxin, additional, Chen, Xueping, additional, Shen, Shuxing, additional, and Zhao, Jianjun, additional

Published
2022
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19. Discovery of aDFRgene that controls anthocyanin accumulation in the spiny Solanum group: roles of a natural promoter variant and alternative splicing

Author

Wang, Xing, primary, Chen, Xueping, additional, Luo, Shuangxia, additional, Ma, Wei, additional, Li, Na, additional, Zhang, Weiwei, additional, Tikunov, Yury, additional, Xuan, Shuxin, additional, Zhao, Jianjun, additional, Wang, Yanhua, additional, Zheng, Gengdi, additional, Yu, Ping, additional, Bai, Yuling, additional, Bovy, Arnaud, additional, and Shen, Shuxing, additional

Published
2022
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20. Construction of a high-density mutant population of Chinese cabbage facilitates the genetic dissection of agronomic traits

Author

Sun, Xiaoxue, primary, Li, Xing, additional, Lu, Yin, additional, Wang, Shan, additional, Zhang, Xiaomeng, additional, Zhang, Kang, additional, Su, Xiangjie, additional, Liu, Mengyang, additional, Feng, Daling, additional, Luo, Shuangxia, additional, Gu, Aixia, additional, Fu, Yu, additional, Chen, Xueping, additional, Xuan, Shuxin, additional, Wang, Yanhua, additional, Xu, Donghui, additional, Chen, Shumin, additional, Ma, Wei, additional, Shen, Shuxing, additional, Cheng, Feng, additional, and Zhao, Jianjun, additional

Published
2022
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22. Genome-Wide Transcriptome Analysis Reveals That Upregulated Expression of Aux / IAA Genes Is Associated with Defective Leaf Growth of the slf Mutant in Eggplant.

Author

Du, Wenchao, Lu, Yang, Luo, Shuangxia, Yu, Ping, Shen, Jiajia, Wang, Xing, Xuan, Shuxin, Wang, Yanhua, Zhao, Jianjun, Li, Na, Chen, Xueping, and Shen, Shuxing

Subjects
EGGPLANT, PLANT biomass, TRANSCRIPTOMES, LEAF development, LEAF growth, GENES, AUXIN, CELL proliferation
Abstract

Leaf size is a crucial trait in eggplant breeding, as it influences photosynthesis, plant biomass and management. However, little is known about the molecular mechanism regulating leaf size in eggplant. This study reports a small leaf mutant (slf) generated with the mutagen ethyl methane sulfonate (EMS). The slf mutant showed restricted cell proliferation and an increased content of auxin. Transcriptome analysis revealed that several genes involved in auxin signaling are upregulated in slf. Exogenous application of auxinole, an auxin antagonist of TIR1/AFB receptors, repressed the expression of these genes and restored leaf growth of slf, suggesting that the small leaf size of slf is likely associated with auxin signaling. This study provides essential clues to unveil the molecular mechanism of leaf size regulation in eggplant. [ABSTRACT FROM AUTHOR]

Published
2022
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26. Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant dwf Dwarfism Is Associated with Gibberellin in Eggplant

Author

Lu, Yang, primary, Luo, Shuangxia, additional, Li, Qiang, additional, Li, Na, additional, Du, Wenchao, additional, Yu, Ping, additional, Wang, Xing, additional, Zhang, Weiwei, additional, Xuan, Shuxin, additional, Zhou, Xuan, additional, Shen, Jiajia, additional, Zhao, Jianjun, additional, Wang, Yanhua, additional, Chen, Xueping, additional, and Shen, Shuxing, additional

Published
2021
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27. Discovery of a DFR gene that controls anthocyanin accumulation in the spiny Solanum group: roles of a natural promoter variant and alternative splicing.

Author

Wang, Xing, Chen, Xueping, Luo, Shuangxia, Ma, Wei, Li, Na, Zhang, Weiwei, Tikunov, Yury, Xuan, Shuxin, Zhao, Jianjun, Wang, Yanhua, Zheng, Gengdi, Yu, Ping, Bai, Yuling, Bovy, Arnaud, and Shen, Shuxing

Subjects
ALTERNATIVE RNA splicing, ANTHOCYANINS, COLOR of plants, PLANT pigments, SOLANUM, EGGPLANT, SINGLE nucleotide polymorphisms
Abstract

SUMMARY: Anthocyanins are important pigments that impart color in plants. In Solanum, different species display various fruit or flower colors due to varying degrees of anthocyanin accumulation. Here we identified two anthocyanin‐free mutants from an ethylmethane sulfonate‐induced mutant library and naturally occurring mutants in Solanum melongena, with mutations in the 5′ splicing site of the second intron of dihydroflavonol‐4‐reductase (DFR) – leading to altered splicing. Further study revealed that alternative splicing of the second intron was closely related to anthocyanin accumulation in 17 accessions from three cultivated species: S. melongena, Solanum macrocarpon and Solanum aethiopicum, and their wild related species. Analysis of natural variations of DFR, using an expanded population including 282 accessions belonging to the spiny Solanum group, identified a single‐nucleotide polymorphism in the MYB recognition site in the promoter region, which causes differential expression of DFR and affects anthocyanin accumulation in fruits of the detected accessions. Our study suggests that, owing to years of domestication, the natural variation in the DFR promoter region and the alternative splicing of the DFR gene account for altered anthocyanin accumulation during spiny Solanum domestication. Significance Statement: Variations in the DFR promoter strongly affect anthocyanin biosynthesis, and alternative splicing of DFR contributes to the diversity of anthocyanin pigmentation during spiny Solanum group domestication. [ABSTRACT FROM AUTHOR]

Published
2022
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28. Development and Application of SSR Markers Related to Genes Involved in Leaf Adaxial-Abaxial Polarity Establishment in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Author

Gao, Ying, primary, Lu, Yin, additional, Li, Xiaoguang, additional, Li, Na, additional, Zhang, Xiaomeng, additional, Su, Xiangjie, additional, Feng, Daling, additional, Liu, Mengyang, additional, Xuan, Shuxin, additional, Gu, Aixia, additional, Wang, Yanhua, additional, Chen, Xueping, additional, Zhao, Jianjun, additional, and Shen, Shuxing, additional

Published
2020
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29. Characterization of Non-heading Mutation in Heading Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Author

Li, Jingrui, primary, Zhang, Xiaomeng, additional, Lu, Yin, additional, Feng, Dongxiao, additional, Gu, Aixia, additional, Wang, Shan, additional, Wu, Fang, additional, Su, Xiangjie, additional, Chen, Xueping, additional, Li, Xing, additional, Liu, Mengyang, additional, Fan, Shuangxi, additional, Feng, Daling, additional, Luo, Shuangxia, additional, Xuan, Shuxin, additional, Wang, Yanhua, additional, Shen, Shuxing, additional, and Zhao, Jianjun, additional

Published
2019
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30. Microspore induced doubled haploids production from ethyl methanesulfonate (EMS) soaked flower buds is an efficient strategy for mutagenesis in Chinese cabbage

Author

Lu, Yin, Dai, Shuangyan, Gu, Aixia, Liu, Mengyang, Wang, Yanhua, Luo, Shuangxia, Zhao, Yujing, Wang, Shan, Xuan, Shuxin, Chen, Xueping, Li, Xiaofeng, Bonnema, Guusje, Zhao, Jianjun, Shen, Shuxing, Lu, Yin, Dai, Shuangyan, Gu, Aixia, Liu, Mengyang, Wang, Yanhua, Luo, Shuangxia, Zhao, Yujing, Wang, Shan, Xuan, Shuxin, Chen, Xueping, Li, Xiaofeng, Bonnema, Guusje, Zhao, Jianjun, and Shen, Shuxing

Abstract

Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products. The greatest embryo production and seedling rates were observed when buds were treated with 0.03 to 0.1% EMS for 5 to 10 min, while EMS concentrations greater than 0.1% were lethal to the microspores. In total, 142 mutants with distinct variations in leaf shape, leaf color, corolla size, flower color, bolting time and downy mildew resistance were identified from 475 microspore culture derived Doubled Haploids. Our results demonstrate that microspore derived Doubled Haploids from EMS soaked buds represents an efficient approach to rapidly generate homozygous Chinese cabbage mutants.

Published
2016

31. Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage

Author

Lu, Yin, primary, Dai, Shuangyan, additional, Gu, Aixia, additional, Liu, Mengyang, additional, Wang, Yanhua, additional, Luo, Shuangxia, additional, Zhao, Yujing, additional, Wang, Shan, additional, Xuan, Shuxin, additional, Chen, Xueping, additional, Li, Xiaofeng, additional, Bonnema, Guusje, additional, Zhao, Jianjun, additional, and Shen, Shuxing, additional

Published
2016
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32. Molecular and genetic regulations of fleshy fruit shape and lessons from Arabidopsisand rice

Author

Li, Qiang, Luo, Shuangxia, Zhang, Liying, Feng, Qian, Song, Lijun, Sapkota, Manoj, Xuan, Shuxin, Wang, Yanhua, Zhao, Jianjun, van der Knaap, Esther, Chen, Xueping, and Shen, Shuxing

Abstract

Fleshy fruit shape is an important external quality trait influencing the usage of fruits and consumer preference. Thus, modification of fruit shape has become one of the major objectives for crop improvement. However, the underlying mechanisms of fruit shape regulation are poorly understood. In this review we summarize recent progress in the genetic basis of fleshy fruit shape regulation using tomato, cucumber, and peach as examples. Comparative analyses suggest that the OFP-TRM (OVATE Family Protein - TONNEAU1 Recruiting Motif) and IQD (IQ67 domain) pathways are probably conserved in regulating fruit shape by primarily modulating cell division patterns across fleshy fruit species. Interestingly, cucumber homologs of FRUITFULL(FUL1), CRABS CLAW(CRC) and 1-aminocyclopropane-1-carboxylate synthase 2(ACS2) were found to regulate fruit elongation. We also outline the recent progress in fruit shape regulation mediated by OFP-TRM and IQD pathways in Arabidopsisand rice, and propose that the OFP-TRM pathway and IQD pathway coordinate regulate fruit shape through integration of phytohormones, including brassinosteroids, gibberellic acids, and auxin, and microtubule organization. In addition, functional redundancy and divergence of the members of each of the OFP, TRM, and IQD families are also shown. This review provides a general overview of current knowledge in fruit shape regulation and discusses the possible mechanisms that need to be addressed in future studies.

Published
2023
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33. Mutation in a chlorophyll-binding motif of Brassicaferrochelatase enhances both heme and chlorophyll biosynthesis

Author

Liu, Mengyang, Ma, Wei, Su, Xiangjie, Zhang, Xiaomeng, Lu, Yin, Zhang, Shaowei, Yan, Jinghui, Feng, Daling, Ma, Lisong, Taylor, Aoife, Ge, Yunjia, Cheng, Qi, Xu, Kedong, Wang, Yanhua, Li, Na, Gu, Aixia, Zhang, Ju, Luo, Shuangxia, Xuan, Shuxin, Chen, Xueping, Scrutton, Nigel S., Li, Chengwei, Zhao, Jianjun, and Shen, Shuxing

Abstract

The heme branch of tetrapyrrole biosynthesis contributes to the regulation of chlorophyll levels. However, the mechanism underlying the balance between chlorophyll and heme synthesis remains elusive. Here, we identify a dark green leaf mutant, dg, from an ethyl methanesulfonate (EMS)-induced mutant library of Chinese cabbage. The dgphenotype is caused by an amino acid substitution in the conserved chlorophyll a/b-binding motif (CAB) of ferrochelatase 2 (BrFC2). This mutation increases the formation of BrFC2 homodimer to promote heme production. Moreover, wild-type BrFC2 and dBrFC2 interact with protochlorophyllide (Pchlide) oxidoreductase B1 and B2 (BrPORB1 and BrPORB2), and dBrFC2 exhibits higher binding ability to substrate Pchlide, thereby promoting BrPORBs-catalyzed production of chlorophyllide (Chlide), which can be directly converted into chlorophyll. Our results show that dBrFC2 is a gain-of-function mutation contributing to balancing heme and chlorophyll synthesis via a regulatory mechanism in which dBrFC2 promotes BrPORB enzymatic reaction to enhance chlorophyll synthesis.

Published
2022
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34. Integratedly analyzed quantitative proteomics with transcriptomics to discover key genes via fg-1 non-heading mutant in the early heading stage of Chinese cabbage.

Author

Li J, Fan M, Zhang X, Yang L, Hou G, Yang L, Li N, Xuan S, and Zhao J

Abstract

Leaf heading is an important agronomic trait of Chinese cabbage, which directly affects its yield. Leaf heading formation in Chinese cabbage is controlled by its internal genotype and external environmental factors, the underlying mechanism of which remains poorly understood. To discover the leaf heading formation mechanism more deeply, this study analyzed the correlation between proteomic and transcriptomic data in the leaf heading formation mutant fg-1 generated by EMS. iTRAQ-based quantitative proteomics techniques were performed to identify the protein expression profiles during the key periods of the early heading stage in the section of the soft leaf apical region (section a) and the whole leaf basal region (section d). We first identified 1,246 differentially expressed proteins (DEPs) in section a and 1,055 DEPs in section d. Notably, transcriptome-proteome integrated analysis revealed that 207 and 278 genes showed consistent trends at the genes' and proteins' expression levels in section a and section d, respectively. KEGG analyses showed that the phenylpropanoid biosynthesis pathway was enriched in both sections a and d. Furthermore, 86 TFs exhibited co-upregulation or co-downregulation, and seven out of 86 were involved in plant hormone synthesis and signal transduction pathways. This indicates that they are potentially related to the leaf heading formation in Chinese cabbage. Taken together, we have identified several key early-heading-formation-related factors via integration analysis of the transcriptomics and proteomics data. This provides sufficient gene resources to discover the molecular mechanism of leaf heading formation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Li, Fan, Zhang, Yang, Hou, Yang, Li, Xuan and Zhao.)

Published
2024
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