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Patients with liver fibrosis who have pain in the liver region may have changed nerve factors. The expression of neurokines and hepatic nerves in liver fibrosis, however, was little understood. In order to better understand how liver fibrosis develops, we plan to look into the hepatic nerve and neurokine changes and how they relate to hepatic stellate cells (HSCs).The expression of neurokines in liver samples from 55 chronic hepatitis B patients and the carbon tetrachloride (CClThe levels of mRNA for neurokines such as BDNF, GFAP, and growth-associated protein (GAP43) are significantly increased in both human and animal liver fibrosis. As liver fibrosis advances, we found that Nissl bodies and α-SMA may co-localize, suggesting a connection between hepatic nerves and HSCs. Human fibrotic serum may increase neurkines, notably BDNF, in SH-SY5Y cells. We also found that BDNF increased pro-inflammatory cytokines and fibrogenic markers in hHSCs.Patients with hepatic fibrosis had significantly higher levels of BDNF, GFAP, GAP43, and nerve fibers. HSC and nerve fibers interact, and nerves also create neurogenic substances that promote liver fibrosis and HSC activation.

OBJECTIVE: To monitor the 3-dimensional (3D) morphological changes of C6/36 cells during dengue virus (DENV) infection using a live-cell imaging technique based on digital holographic microscopy and provide clues for better understanding the mechanisms of DENV infection. METHODS: C6/36 cells were seeded in 6-well plates to determine the optimal imaging density under a holographic cell imager, and the morphological changes of the cells were recorded in response to a culture temperature change from 28 ℃ to 37 ℃. C6/36 cells were infected with 4 DENV strains with different serotypes at 28 ℃ and incubated at 37 ℃ for 24 h, and the 3D holograms and relevant morphological parameters were recorded at different time points using HoloMonitor M4 holographic cell imaging and analysis system. RESULTS: The holograms of C6/36 cells inoculated at the optimal density for imaging (4 × 10(5) per well) showed unified 3D morphologies of the single cells with minimal dispersions in the cell area, thickness and volume (P0.05). The cell area and volume of the cells infected with the 4 DENV strains all increased and the cell thickness was reduced during incubation. Among the 4 strains, DENV-1 and DENV-2 caused reduced cell thickness while DENV-3 and DENV-4 increased the cell thickness, and the pattern and degree of such changes differ among the 4 strains. CONCLUSION: s Digital holographic microscopy allows monitoring of the complex morphological changes of cells during DENV infection. The 4 DENV strains with different serotypes causes characteristic cell damages during infection.

Using basic physical arguments, we present a design and method for the fabrication of a pneumatic micro PDMS actuator using multilayer soft lithography based on dry-film photoresist. Its novel structure consists of a three-way electromagnetic microvalve for gas flow control which is connected with gas inlet microchannel for the actuator. Then the whole system is considered as a throttle blind capacitor (TBC) model and it is used to predict the response time of the pneumatic actuator by correlating its characteristics such as gas pressurizing, air resistance and membrane deformation. For this micro actuator, upward deformation motions of actuated area are observed of actuated area under 100KPa (N2) pressure and the fast open response (~ 15 ms) was achieved at 50KPa pressure. Our method provides a simple, cheap and save-time pneumatic micro actuator which can be used in active functions, such as valves, pumps and mixers integrated on pneumatic microfluidic chips for downsizing the driving pressure and improving the whole efficiency of microchips.

With the increasing development of society and the economy, the concept of green construction gradually attracts the much attention, and has been more widely used. In the green construction process, materials and energy-saving is a very important aspect. The rapid development in information technology today, information technology in all walks of life have been fully applied. The digital technology has provided us with the opportunity of a construction design in a virtual environment. This paper first describes the concept of green construction, and then expounds the specific of technology on utilization of green construction material-saving and material resources,through combining with specific examples, expounds the specific benefits of green construction.

Construction project management is a method system that takes whole process and all-around control and management of the construction project engineer. The characteristics of both the common project management mode and the new innovation mode are discussed in this passage. Put forward construction project and responsibility system management mode after having discussed how to innovation building project management mode.

To investigate the effect of ginsenoside Rb1 on cerebral infarction volume as well as IL-1 beta in the brain tissue and sera of focal cerebral ischemia/reperfusion (I/R) injury model rats.The I/R rat model was established by using thread according to Zea-Longa. SD rats were randomly divided into five groups, i.e., the sham-operation group, the model group, the low dose ginsenoside Rb1 (20 mg/kg) group, the medium dose ginsenoside Rb1 group (40 mg/kg), and the high dose ginsenoside Rb1 group (80 mg/kg), 12 in each group. Rats in the sham-operation group only received middle cerebral artery occlusion (MCAO) but without thread insertion. The MCAO model was prepared in the rest 4 groups, followed by MCAO2 h later. Ginsenoside Rb1 at each dose was peritoneally administrated to rats in corresponding groups immediately after cerebral ischemia. Equal volume of normal saline was administered to rats in the sham-operation group. Rats' cerebral infarction volume, integrals of neurologic defect degree, expression of IL-1 beta content in the brain tissue and sera were observed 24 h after 2-h cerebral I/R.In the model group, integrals of neurologic defect degree were improved (P0.01), IL-1 beta positive cells in the brain tissue increased and serum IL-1 beta content elevated (P0.05), when compared with the sham-operation group. In comparison of the model group, integrals of neurologic defect degree were lowered in the medium dose and high dose ginsenoside Rb1 groups (P0.05, P0.01). The cerebral infarction volume was all shrunken in each ginsenoside Rb1 group, IL-1 beta positive cells in the brain tissue decreased, and IL-1 beta content in serum reduced (P0.01, P0.05). Compared with the low dose ginsenoside Rb1 group, integrals of neurologic defect degree decreased, the cerebral infarction volume shrunken, and IL-1 beta content in serum reduced in the high dose ginsenoside Rb1 group (P0.01, P0.05).Ginsenoside Rb1 (20, 40, 80 mg/kg) might effectively release local cerebral ischemia by down-regulating the IL-1 beta expression.

Recently, various social circles are paying special attention to the great tourism festival activities about the ritual for worshiping the ancestor in Yellow Emperor's hometown. The success of the rituals fully showed the advantageous tourist resources in Henan, promoted the culture of Yellow emperor, inherited the historical culture, enriched the tourism culture. Some innovative suggestions have been made pointing at the problem that can exist in the ritual to reach the branding and marketing operation and to provide the development of tourism festival in china with a new mode.