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Median palatal cyst is a rare nonodontogenic facial cleft cyst. A case of median palatal cyst was admitted to the Department of Otolaryngology, Renji Hospital. A 26-year-old female patient was admitted with ‘repeated bilateral nasal congestion for two years’ and was diagnosed as a median palatal cyst. Endoscopic resection of the cyst at the bottom of the nose plus free nasal septal cartilage transplantation were performed. The wound healed well and the cyst did not recur after one year of follow-up.

Alterations in the tumor microenvironment are closely associated with the metabolic phenotype of tumor cells. Cancer-associated fibroblasts (CAFs) play a pivotal role in tumor growth and metastasis. Existing studies have suggested that lactate produced by tumor cells can activate CAFs, yet the precise underlying mechanisms remain largely unexplored. In this study, we initially identified that lactate derived from lung cancer cells can promote nuclear translocation of NUSAP1, subsequently leading to the recruitment of the transcriptional complex JUNB-FRA1-FRA2 near the DESMIN promoter and facilitating DESMIN transcriptional activation, thereby promoting CAFs' activation. Moreover, DESMIN-positive CAFs, in turn, secrete IL-8, which recruits TAMs or promotes M2 polarization of macrophages, further contributing to the alterations in the tumor microenvironment and facilitating lung cancer progression. Furthermore, we observed that the use of IL-8 receptor antagonists, SB225002, or Navarixin, significantly reduced TAM infiltration and enhanced the therapeutic efficacy of anti-PD-1 or anti-PD-L1 treatment. This finding indicates that inhibiting IL-8R activity can attenuate the impact of CAFs on the tumor microenvironment, thus restraining the progression of lung cancer.

Abstract In this letter, molybdenum nitride (MoN) gate AlGaN/GaN HEMT has been investigated with MoN developed in various physical vapour deposition (PVD) conditions. The Schottky gate is studied through forward and reverse characteristics with different processed MoN, which is compared with the standard Ni gate. Similar DC and radio frequency (RF) performances are obtained with MoN and Ni gate, while MoN demonstrated higher reliability than Ni gate through RF aging test.

PGC1α, a central player in mitochondrial biology, holds a complex role in the metabolic shifts seen in cancer cells. While its dysregulation is common across major cancers, its impact varies. In some cases, downregulation promotes aerobic glycolysis and progression, whereas in others, overexpression escalates respiration and aggression. PGC1α’s interactions with distinct signaling pathways and transcription factors further diversify its roles, often in a tissue-specific manner. Understanding these multifaceted functions could unlock innovative therapeutic strategies. However, challenges exist in managing the metabolic adaptability of cancer cells and refining PGC1α-targeted approaches. This review aims to collate and present the current knowledge on the expression patterns, regulators, binding partners, and roles of PGC1α in diverse cancers. We examined PGC1α’s tissue-specific functions and elucidated its dual nature as both a potential tumor suppressor and an oncogenic collaborator. In cancers where PGC1α is tumor-suppressive, reinstating its levels could halt cell proliferation and invasion, and make the cells more receptive to chemotherapy. In cancers where the opposite is true, halting PGC1α’s upregulation can be beneficial as it promotes oxidative phosphorylation, allows cancer cells to adapt to stress, and promotes a more aggressive cancer phenotype. Thus, to target PGC1α effectively, understanding its nuanced role in each cancer subtype is indispensable. This can pave the way for significant strides in the field of oncology.

Medium–long-term photovoltaic (PV) output forecasting is of great significance to power grid planning, power market transactions, power dispatching operations, equipment maintenance and overhaul. However, PV output fluctuates greatly due to weather changes. Furthermore, it is frequently challenging to ensure the accuracy of forecasts for medium–long-term forecasting involving a long time span. In response to the above problems, this paper proposes a medium–long-term forecasting method for PV output based on amplitude-aware permutation entropy component reconstruction and the graph attention network. Firstly, the PV output sequence data are decomposed by ensemble empirical mode decomposition (EEMD), and the decomposed intrinsic mode function (IMF) subsequences are combined and reconstructed according to the amplitude-aware permutation entropy. Secondly, the graph node feature sequence is constructed from the reconstructed subsequences, and the mutual information of the node feature sequence is calculated to obtain the graph node adjacency matrix which is applied to generate a graph sequence. Thirdly, the graph attention network is utilized to forecast the graph sequence and separate the PV output forecasting results. Finally, an actual measurement system is used to experimentally verify the proposed method, and the outcomes indicate that the proposed method, which has certain promotion value, can improve the accuracy of medium–long-term forecasting of PV output.

Background: This study aimed to evaluate whether dexmedetomidine (DEX) can relieve postoperative pain and reduce stress reaction after laparoscopic uterine fibroids (UF) surgery. Methods: This randomized controlled study included 100 patients age range of 40–60 years, American Society of Anesthesiologists (ASA) 1–2 grade, admitted for laparoscopic UF surgery from January 2020 to October 2023. The patients were randomly allocated to the DEX group (n = 50) and non-DEX group (n = 50). The visual analogue scale (VAS), Richmond Agitation-Sedation Scale (RASS) score, anesthesia time, spontaneous breathing recovery time, consciousness recovery time, extubation time, and recovery room time were recorded. Results: The VAS of the DEX group was smaller than that of the non-DEX group at half an hour, but there was no significant difference in VAS at 1 h, 12 h and 24 h. Dex group has a RASS value closer to zero than non-DEX group at half an hour. There was no significant difference in RASS at 1 h, 12 h, or 24 h. There were differences in spontaneous breathing recovery time, consciousness recovery time, extubation time, recovery room time, chills and nausea between the two groups. Conclusion: Application of DEX in perioperative period can relieve postoperative pain, reduce stress reaction after laparoscopic UF surgery. Clinical Trial Registration: The study has been registered on https://classic.clinicaltrials.gov/ (registration number: NCT03524950).

Abstract The Jiaozhou Bay ecosystem, a crucial marine ecosystem in China, has been plagued by frequent harmful algal blooms as due to deteriorating water quality and eutrophication. This study analyzed the temporal and spatial changes of harmful algal blooms in Jiaozhou Bay from 2000 to 2022 using the Floating Algae Index (FAI) calculated from MODIS (2000–2022) and Sentinel-2 (2015–2022) satellite image datasets. The calculation results of the image datasets were compared. The frequency of planktonic algal outbreaks was low and constant until 2017, but has increased annually since then. Algae blooms are most common in the summer and primarily concentrated along the bay’s coast, middle, and mouth, with obvious seasonal and spatial distribution characteristics. Several factors influencing algal outbreaks were identified, including sea surface temperature, wind speed, air pressure, dissolved oxygen, nitrogen and phosphorus ratios, chemical oxygen demand, and petroleum pollutants. Algal bloom outbreaks in Jiaozhou Bay are expected to remain high in 2023. The findings provide crucial information for water quality management and future algal outbreak prediction and prevention in Jiaozhou Bay.

Abstract Background Drug resistance limits the treatment effect of cisplatin-based chemotherapy in head and neck squamous cell carcinoma (HNSCC), and the underlying mechanism is not fully understood. The aim of this study was to explore the cause of cisplatin resistance in HNSCC. Methods We performed survival and gene set variation analyses based on HNSCC cohorts and identified the critical role of tumor necrosis factor alpha-induced protein 2 (TNFAIP2) in cisplatin-based chemotherapy resistance. Half-maximal inhibitory concentration (IC50) examination, colony formation assays and flow cytometry assays were conducted to examine the role of TNFAIP2 in vitro, while xenograft models in nude mice and 4-nitroquinoline N-oxide (4NQO)-induced HNSCC models in C57BL/6 mice were adopted to verify the effect of TNFAIP2 in vivo. Gene set enrichment analysis (GSEA) and coimmunoprecipitation coupled with mass spectrometry (Co-IP/MS) were performed to determine the mechanism by which TNFAIP2 promotes cisplatin resistance. Results High expression of TNFAIP2 is associated with a poor prognosis, cisplatin resistance, and low reactive oxygen species (ROS) levels in HNSCC. Specifically, it protects cancer cells from cisplatin-induced apoptosis by inhibiting ROS-mediated c-JUN N-terminal kinase (JNK) phosphorylation. Mechanistically, the DLG motif contained in TNFAIP2 competes with nuclear factor-erythroid 2-related factor 2 (NRF2) by directly binding to the Kelch domain of Kelch-like ECH-associated protein 1 (KEAP1), which prevents NRF2 from undergoing ubiquitin proteasome-mediated degradation. This results in the accumulation of NRF2 and confers cisplatin resistance. Positive correlations between TNFAIP2 protein levels and NRF2 as well as its downstream target genes were validated in HNSCC specimens. Moreover, the small interfering RNA (siRNA) targeting TNFAIP2 significantly enhanced the cisplatin treatment effect in a 4NQO-induced HNSCC mouse model. Conclusions Our results reveal the antioxidant and cisplatin resistance-regulating roles of the TNFAIP2/KEAP1/NRF2/JNK axis in HNSCC, suggesting that TNFAIP2 might be a potential target in improving the cisplatin treatment effect, particularly for patients with cisplatin resistance.

Accurate control of liquid–liquid interfaces is of great importance in many scientific fields. Currently, most studies on liquid–liquid interfaces are based on microfluidics in closed channels, and for open systems, it is difficult to form stable liquid–liquid interfaces in microchannels due to the interference of gas-phase molecules. Here, we introduce a new method to manipulate the motion of the liquid–liquid interface under an open microchannel. Under the effect of surface tension, the liquid–liquid interface moves in microchannels until it encounters microstructures in the microchannels, where the force equilibrates and remains stable to form a fixed shape. The shape of the interface is regulated by adjusting the dimensions of the microchannels and microstructures as well as the positions of the microstructures in the microchannels. This spontaneous liquid–liquid interface moves, stops, and remains stable in a very convenient way. The morphology and position of the interface are well manipulated using microstructures, and the liquid–liquid interface with well-defined geometry can be made to stay in different positions to play different roles.

Background: Allergic parotitis (AP), due to its non-specific symptoms, frequently poses a diagnostic challenge, leading to cases being overlooked or misdiagnosed by clinicians. Objective: This study aimed to elucidate detailed clinical characteristics and common diagnostic indicators of AP. Methods: A comprehensive review and analysis of medical records was conducted from patients diagnosed with AP, encompassing demographic, clinical, and laboratory data, at the Affiliated Stomatological Hospital of Nanjing Medical University between January 2019 and March 2022. Results: The study enrolled 17 patients, evidenced by an average age of 36.00 ± 12.95 years. Common presentations of AP among the patients included notable symptoms such as parotid gland swelling, associated pain, and xerostomia. Ten patients had other atopic diseases. Palpation revealed the affected parotid glands to be soft and nodular, with an elevated local skin temperature. The unstimulated whole saliva flow rate was decreased. Ultrasonography demonstrated increased volume, reduced echo heterogeneity, and lymph node enlargement in the affected parotid glands. All cases observed increased serum salivary amylase and total IgE levels. Investigation of food allergens and inhaled allergen-specific IgE showed that all patients had suspected food allergies. Food provocation tests (FPT) induced AP in 13 cases, confirming the role of food allergens. Conclusion: Food allergens are involved in the etiology of AP, underscoring the importance of comprehensive clinical evaluation, including symptoms, signs, and confirmatory auxiliary tests, such as FPT, for accurate diagnosis and differentiation from other salivary gland pathologies.

Neutron absorption gratings play a crucial role in neutron phase contrast imaging systems, where the fabrication of large-size and small-period absorption gratings that meet imaging requirements presents a significant challenge. The pressurized particle filling method has been successfully applied to fabricate large-size absorption gratings. Here, we investigated the feasibility of the pressurized particle filling method for fabricating small-period gratings and proposed an optimized pressurized particle filling method. The grating surface was covered with a uniform particle layer and then pressurized, utilizing the adaptive deformation of the particle layer to achieve uniform particle filling. Neutron absorption gratings with an area of 60 × 60 mm2 and periods of 8 and 4 µm were fabricated through this method. The particle filling rate and fabrication efficiency were successfully improved. In addition, the evaluation of the particle filling uniformity method by analyzing the proportion of particles on the grating surface was proposed. The better uniformity of small-period neutron absorption gratings indicated that the optimized pressurized particle filling method can achieve relatively uniform particle filling.

Intraneuronal inclusions of alpha-synuclein (α-synuclein, α-syn) are commonly found in the brain of patients with Parkinson's disease (PD). The pathogenesis of the abundant α-syn protein in the blood has been extensively studied to understand its properties better. In recent years, peptidome analysis has received increasing attention. In this study, we identified and analyzed serum peptides from wild-type (WT) and the (Thy-1)-h[A30P] alpha-synuclein transgenic mice (SNCA-A30P mice) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). One thousand eight hundred fifty-six peptides from 771 proteins were analyzed. Among them, 151 peptides from 107 proteins were significantly differentially expressed. The glycoprotein VI platelet pathway (GP6) was the pathway's most significant differentially expressed signaling pathway. Cleavage sites of the differentially expressed peptides may reflect protease distribution and activity. We selected the most significantly differentially expressed peptide, VGGDPI, and found that it contained cathepsin K (Ctsk) and trypsin-1 cleavage sites, suggesting that Ctsk and trypsin-1 may be key peptidases in PD. α-syn is a protein associated with the pathogenesis of PD. mutations in several genes, including SNCA, which encodes α-syn, are associated with the development of PD. Bioinformatics analysis of the physiological pathways related to SNCA genes and apoptosis genes found the five most markedly up-regulated proteins: formin homology 2 domain-containing 1 (FHOD1), insulin receptor substrate 1(IRS1), TRPM8 channel-associated factor 1 (TCAF1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and interleukin-16 (IL-16). Therefore, the differentially expressed peptides in the five precursor protein domains may be potential bioactive peptides associated with α-syn and apoptosis. This study provides a validated peptidomics profile of SNCA-A30P mice and identifies potentially bioactive peptides linked to α-syn and apoptosis.

Abstract Background Massive hemoptysis is a life-threatening condition that requires immediate treatment. This study aimed to retrospectively analyze the outcome of bronchial artery embolization (BAE) for massive hemoptysis, as well as potential factors that may contribute to the recurrence of hemoptysis after BAE. Methods A total of 105 patients with massive hemoptysis treated with BAE were analyzed. Results The immediate control rate of bleeding was 84.8% (67/79); however, during the 36-month follow-up, 45.3% (29 out of 64) of the patients had recurrent hemoptysis after BAE. Comorbidities, pituitary hormone treatment, the angiographic appearance of artery dilation and hypertrophy, and the materials used for BAE were significantly correlated with the success rate of the BAE, while lack of pituitary hormone treatment and existence of arterio-arterial or arteriovenous fistula were risk factors for the recurrence of hemoptysis after BAE. Only a small proportion of patients (9/105, 8.6%) had mild complications after BAE treatment. Conclusion Findings suggest that BAE continues to be an effective treatment for massive hemoptysis in emergency settings. Moreover, the treatment of underlying pulmonary diseases and comorbidities is important to increase BAE’s success rate of BAE and decrease the risk of recurrent hemoptysis after BAE.

Background: The aim of this study was to determine whether dexmedetomidine can reduce the circulatory response in laparoscopic uterine fibroids (UF) surgery. Our purpose was to investigate the effect of dexmedetomidine on the circulatory response in laparoscopic UF surgery. Methods: This was a randomized controlled study of 214 patients age range of 40–60 years, American Society of Anesthesiologists (ASA) 1–2 grade, undergoing elective laparoscopic UF surgery at Fuxing Hospital, Capital Medical University from January 2020 to October 2023. Patients were randomly allocated to the non-dexmedetomidine group (Group1) and dexmedetomidine group (Group2). The mean arterial pressure (MAP) and heart rate (HR) were measured: when entering the operation room (MAP1, HR1), immediately at the time of local pituitrin injection (MAP2, HR2), and at 5 minutes after injection of pituitrin (MAP3, HR3). The medications and dosages (propofol, urapidil hydrochloride, fluid) used during the operation were recorded as well as the recovery (drowsiness, chills, nausea, vomiting, dysphoria) after the operation. Total mean time from the beginning of the operation to pituitrin injection (Time1) and recovery time after peaking blood pressure with pituitrin injection (Time2) were also recorded. Results: There were no significant differences in age, height, weight, size and number of UFs, and the location of uterine fibroids between the two groups. There were no differences between the groups for MAP1, MAP2, HR1, HR2, HR3, and Time1 (all p > 0.05), but the dexmedetomidine group was smaller in MAP3 (p = 0.041) and Time2 (p = 0.000) than the non-dexmedetomidine group. There were significant differences in MAP3-MAP1 (p = 0.025), propofol (p = 0.011), and urapidil hydrochloride (p = 0.000) between the two groups. The dexmedetomidine group was smaller in chills (p = 0.002), nausea (p = 0.000), vomiting (p = 0.04), and dysphoria (p = 0.024) than the non-dexmedetomidine group. Conclusions: Dexmedetomidine maintains a stable hemodynamic response and is beneficial to postoperative recovery in laparoscopic UF surgery. Clinical Trial Registration: The study has been registered on https://classic.clinicaltrials.gov/ (registration number: NCT03524950).

Blockchain is a viable solution to provide data integrity for the enormous volume of 5G IoT social data, while we need to break through the throughput bottleneck of blockchain. Sharding is a promising technology to solve the problem of low throughput in blockchains. However, cross-shard communication hinders the effective improvement of blockchain throughput. Therefore, it is critical to reasonably allocate transactions to different shards to improve blockchain throughput. Existing research on blockchain sharding mainly focuses on shards formation, configuration, and consensus, while ignoring the negative impact of cross-shard communication on blockchain throughput. Aiming to maximize the throughput of transaction processing, we study how to allocate blockchain transactions to shards in this paper. We propose an Associated Transaction assignment algorithm based on Closest Fit (ATCF). ATCF classifies associated transactions into transaction groups which are then assigned to different shards in the non-ascending order of transaction group sizes periodically. Within each epoch, ATCF tries to select a shard that can handle all the transactions for each transaction group. If there are multiple such shards, ATCF selects the shard with the remaining processing capacity closest to the number of transactions in the transaction group. When no such shard exists, ATCF chooses the shard with the largest remaining processing capacity for the transaction group. The transaction groups that cannot be completely processed within the current epoch will be allocated in the subsequent epochs. We prove that ATCF is a 2-approximation algorithm for the associated transaction assignment problem. Simulation results show that ATCF can effectively improve the blockchain throughput and reduce the number of cross-shard transactions.

ABSTRACT Recent evidence emphasized the role of the microbiota in the etiopathogenesis of rheumatoid arthritis (RA). Indeed, it has been demonstrated that urinary tract infections are implicated in RA pathogenesis. However, a definitive association between the urinary tract microbiota and RA remains to be investigated. Urine samples from 39 patients affected by RA, including treatment-naive patients, and 37 age- and sex-matched healthy individuals were collected. In RA patients, the urinary microbiota showed an increase in microbial richness and a decrease in microbial dissimilarity, especially in treatment-naive patients. A total of 48 altered genera with different absolute quantities were detected in patients with RA. The 37 enriched genera included Proteus, Faecalibacterium, and Bacteroides, while the 11 deficient genera included Gardnerella, Ruminococcus, Megasphaera, and Ureaplasma. Notably, the more abundant genera in RA patients were correlated with the disease activity score of 28 joints-erythrocyte sedimentation rates (DAS28-ESR) and an increase in plasma B cells. Furthermore, the altered urinary metabolites, such as proline, citric acid, and oxalic acid, were positively associated with RA patients, and they were closely correlated with urinary microbiota. These findings suggested a strong association between the altered urinary microbiota and metabolites with disease severity and dysregulated immune responses in RA patients. IMPORTANCE We revealed that the profile of the urinary tract microbiota in RA featured with increased microbial richness and shifted taxa, associated with immunological and metabolic changes of the disease, underlining the interplay between urinary microbiota and host autoimmunity.

Detached eclipsing binary (EB) systems are crucial for measuring the physical properties of stars that evolve independently. Large-scale time-domain surveys have released a substantial number of light curves for detached EBs. Utilizing the Physics of Eclipsing Binaries package in conjunction with Markov Chain Monte Carlo (MCMC) methods for batch parameter derivation poses significant computational challenges, primarily due to the high computational cost and time demands. Therefore, this paper develops an efficient method based on the neural network model and the stochastic variational inference method (denoted NNSVI) for the rapid derivation of parameters for detached EBs. For studies involving more than three systems, the NNSVI method significantly outperforms techniques that combine MCMC methods in terms of parameter inference speed, making it highly suitable for the batch derivation of large numbers of light curves. We efficiently derived parameters for 34,907 detached EBs, selected from the Optical Gravitational Lensing Experiment catalog and located in the Galactic bulge, using the NNSVI method. A catalog detailing the parameters of these systems is provided. Additionally, we compared the parameters of two double-lined detached EBs with those from previous studies and found the estimated parameters to be essentially identical.

TIC 157365951 has been classified as a δ Scuti type by the International Variable Star Index. Through the spectra from Large Sky Area Multi-Object Fiber Spectroscopic Telescope and its light curve, we further discovered that it is a binary system. This binary system comprises a red-dwarf star and a compact star. Through the spectral energy distribution fitting, we determined the mass of the red dwarf star as M _1 = 0.31 ± 0.01 M _⊙ and its radius as R _1 = 0.414 ± 0.004 R _⊙ . By fitting the double-peaked H α emission, we derived the mass ratio of q = 1.76 ± 0.04, indicating a compact star mass of M _2 = 0.54 ± 0.01 M _⊙ . Using Phoebe to model the light curve and radial velocity curve for the detached binary system, we obtained a red dwarf star mass of M _1 = 0.29 ± 0.02 M _⊙ , a radius of R _1 = 0.39 ± 0.04 R _⊙ , and a Roche–lobe filling factor of f = 0.995 ± 0.129, which is close to the f = 1 expected for a semidetached system. The Phoebe model gives a compact star mass M _2 = 0.53 ± 0.05 M _⊙ . Constraining the system to be semidetached gives M _1 = 0.34 ± 0.02 M _⊙ , R _1 = 0.41 ± 0.01 R _⊙ , and M _2 = 0.62 ± 0.03 M _⊙ . The consistency of the models is encouraging. The value of the Roche–lobe filling factor suggests that there might be ongoing mass transfer. The compact star mass is as massive as a typical white dwarf.

With the continuous development of large optical surveys, a large number of light curves of late-type contact binary systems (CBs) have been released. Deriving parameters for CBs using the the Wilson–Devinney program and the PHOEBE program poses a challenge. Therefore, this study developed a method for rapidly deriving light curves based on the Neural Networks model combined with the Hamiltonian Monte Carlo (HMC) algorithm (NNHMC). The neural network was employed to establish the mapping relationship between the parameters and the pregenerated light curves by the PHOEBE program, and the HMC algorithm was used to obtain the posterior distribution of the parameters. The NNHMC method was applied to a large contact binary sample from the Catalina Sky Survey, and a total of 19,104 late-type contact binary parameters were derived. Among them, 5172 have an inclination greater than 70° and a temperature difference less than 400 K. The obtained results were compared with the previous studies for 30 CBs, and there was an essentially consistent goodness-of-fit ( R ^2 ) distribution between them. The NNHMC method possesses the capability to simultaneously derive parameters for a vast number of targets. Furthermore, it can provide an extremely efficient tool for the rapid derivation of parameters in future sky surveys involving large samples of CBs.

A contact binary may be the progenitor of a red nova that eventually produces a merger event and have a cut-off period of around 0.2 days. Therefore, a large number of contact binaries is needed to search for the progenitor of red novae and to study the characteristics of short-period contact binaries. In this paper, we employ the Phoebe program to generate a large number of light curves based on the fundamental parameters of contact binaries. Using these light curves as samples, an autoencoder model is trained, which can reconstruct the light curves of contact binaries very well. When the error between the output light curve from the model and the input light curve is large, it may be due to other types of variable stars. The goodness of fit ( R ^2 ) between the output light curve from the model and the input light curve is calculated. Based on the thresholds for global goodness of fit ( R ^2 ), period, range magnitude, and local goodness of fit ( R ^2 ), a total of 1322 target candidates were obtained.

Low-mass-ratio ( q ) contact binary systems are progenitors of stellar mergers such as blue stragglers or fast-rotating FK Com stars. In this study, we present the first light curve analysis of two newly identified low mass-ratio contact binary systems, TIC 55007847 and TIC 63597006, that are identified from TESS. Both stars are classified as A-subtype contact binaries. We obtained the precise orbit periods for the two objects by using the O − C method, i.e., P = 0.6117108 day for TIC 55007847 and P = 0.7008995 day for TIC 63597006, and found an obvious periodic signal in the O − C curve of TIC 63597006. We suggest that the periodic signal comes from a third body. We further use the Markov chain Monte Carlo method with PHOEBE to derive the photometric solutions for the two binaries. The photometric solution for this object shows that the contribution of the third body is about 6%. Our analysis revealed that TIC 55007847 has an extremely low mass ratio of q = 0.08. By calculating the ratio of spin angular momentum to the orbital angular momentum J _s / J _o , we found that TIC 55007847 is very close to the instability threshold with J _s / J _o = 0.31, indicating that it may merge into a single, fast-rotating star in the future. For TIC 63597006, q = 0.14 and J _s / J _o = 0.15. This object is in a relatively stable evolutionary status at present.

Starting from more than 11,200 short-period (less than 0.5 days) EW-type eclipsing binary candidates with the All-Sky Automated Survey for Supernovae V -band light curves, we use the Markov Chain Monte Carlo algorithm and neural networks to obtain the mass ratio ( q ), orbital inclination ( incl ), fill-out factor ( f ), and temperature ratio ( T _s / T _p ). After crossmatching with the Gaia DR3 database, the final sample contains parameters of 2399 A-type and 8712 W-type contact binaries (CBs). We present the distributions of parameters of these 11,111 short-period CBs. The mass ratio ( q ) and fill-out factor ( f ) are found to obey log-normal distributions, and the remaining parameters obey normal distributions. There is a significant period–temperature correlation of these CBs. Additionally, the temperature ratio ( T _s / T _p ) tends to increase as the orbital period decreases for W-type CBs. There is no significant correlation between them for A-type CBs. The mass ratio and fill-out factor ( q − f ) diagram suggest there is no significant correlation between these two parameters. A clear correlation exists between the mass ratio and radius ratio. The radius ratio increases with the mass ratio. Moreover, the deep fill-out CBs tend to fall on the upper boundary of the q − R _s / R _p distribution, while the shallow fill-out CBs fall on the lower boundary.

Semidetached binaries are in the stage of mass transfer and play a crucial role in studying the physics of mass transfer between interacting binaries. Large-scale time-domain surveys provide many light curves of binary systems, while Gaia offers high-precision astrometric data. In this paper, we develop, validate, and apply a pipeline that combines the Markov Chain Monte Carlo method with a forward model and DBSCAN clustering to search for semidetached binaries and estimate the inclination, relative radius, mass ratio, and temperature ratio of each using light curves. We train our model on the mock light curves from Physics of Eclipsing Binaries (PHOEBE), which provides broad coverage of light-curve simulations for semidetached binaries. Applying our pipeline to Transiting Exoplanet Survey Satellite sectors 1–26, we have identified 77 semidetached binary candidates. Utilizing the distance from Gaia, we determine their masses and radii with median fractional uncertainties of ∼26% and ∼7%, respectively. With the added 77 candidates, the catalog of semidetached binaries with orbital parameters has been expanded by approximately 20%. The comparison and statistical results show that our semidetached binary candidates align well with the compiled samples and the PARSEC model in T _eff – L and M – R relations. Combined with the literature samples, comparative analysis with stability criteria for conserved mass transfer indicates that ∼97.4% of samples are undergoing nuclear-timescale mass transfer, and two samples (GO Cyg and TIC 454222105) are located within the limits of stability criteria for dynamical- and thermal-timescale mass transfer, and are currently undergoing thermal-timescale mass transfer. Additionally, one system (IR Lyn) is very close to the upper limit of delayed dynamical-timescale mass transfer.

In this paper, permeable geopolymer concrete with high compressive strength and permeability is prepared using alkali-activated metakaolin as a slurry, and its mechanical properties are reinforced by adding steel fibers. The influencing factors of the strength, porosity and permeability coefficient of the fiber-reinforced permeable geopolymer concrete, as well as its microstructure and curing mechanism, are determined by conducting an unconfined compressive strength test, scanning electron microscopy, energy-dispersive spectroscopy and X-ray diffraction. The test results show that, under the water permeability required to meet the specification conditions, when the alkali activator modulus is 1.4 and the activation-to-solid ratio is 0.9, the effect of metakaolin activation is the most obvious, and the unconfined compressive strength of the permeable geopolymer concrete is the highest. Moreover, the paste formed via the alkali activation of metakaolin contains a large number of silica–oxygen and aluminum–oxygen bonds with a dense and crack-free structure, which enables the paste to tightly combine with the aggregates; the strength of the permeable geopolymer concrete is early strength, and its strength at a curing age of 3 days is the highest. The strength at a curing age of 3 days can reach 43.62% of the 28-day strength; the admixture of steel fiber can effectively improve the strength of the permeable concrete, and with an increase in the amount of admixture, the strength of the fiber shows a trend of increasing, and then decreasing. Under the conditions of this test, a volume of steel fiber of 0.3% enables the optimum unconfined compressive strength to be reached.

Using 1979 -2014 Global Land Data Assimilation System-the Community surface parameter data set， China regional daily observation data grid data set （CN05.1） and ERA-interim reanalyze atmospheric circulation data.To study the relationship between the abnormal soil moisture in the Qinghai-Xizang Plateau in May and the surface heat flux in June and the precipitation change in July.The results show that： （1） From 1979 to 2014， the average soil moisture in the 0~10 cm layer area in May was abnormally high years were： 2000， 2001， 2004， 2005， 2006， 2013； the abnormal low years were： 1994， 1995， 1996， 1998， 1999.Soil moisture increased after 2000.Soil moisture increased after 2000.The soil moisture in the higher Tibetan Plateau， the Three Rivers Source Region， and the southern Tibetan valley is significantly higher than in the lower years.The results passed the 90% confidence test.（2） There is a clear correlation between the abnormality of soil moisture in spring and the distribution of summer precipitation in China.When the soil is wet （dry）， from high latitude to low latitude， the relevant area is "+ - + -" （"- + - +"） banded distribution.（3） The soil is moist in May， and the sensible heat flux in the east and the latent heat flux in the west increase in June.Their combined effect strengthens the convergent circulation of the lower atmosphere and the divergence circulation of the upper layer， so that the East Asia region is controlled by the strong anticyclone circulation above 850 hPa.The 500 hPa and above in the northeast of the plateau is an anticyclone circulation， and the south and west are cyclonic circulations.This circulation field configuration makes the South Asian high pressure move eastward and the Western Pacific subtropical high pressure.（4） In July， the Huanghuai area on the north side of the Pacific High has a vertical upward movement.The warm and moist air flow converges with the dry and cold air flow on the west side of the northeast cold vortex.High-level divergence and low-level convergence are conducive to precipitation in Huanghuai area.The eastern northwestern and northern China regions are controlled by anticyclonic circulation， with strong sinking movement， dry and cold air， and insufficient water vapor transport， which is unfavorable for precipitation.

To clarify the plastic law and hardening characteristics of deformation and failure of staggered zone under high in-situ stress unloading conditions with excavation in underground engineering, a series of triaxial tests under different confining pressures and different loading and unloading stress paths was conducted. The plastic deformation laws of the staggered zone under different loading and unloading stress paths were deeply investigated. Based on the results of the experiments, the dependency of stress path of internal variables such as the equivalent plastic work, the equivalent plastic strain and the plastic volumetric strain as hardening parameters were further explored in the stress space. Meanwhile, a modified formula for hardening parameters with the independency of stress path was proposed. The results showed that, 1) The effect of stress path was significant on the deformation properties of staggered zone under high in-situ stress. The high initial confining pressure would inhibit the development of the circumferential plastic deformation of samples with the staggered zone under the same unloading stress path. And the plastic volumetric residual strain (6%) of samples with the staggered zone under the stress path of unloading axial pressure and confining pressure was significantly greater than that of other unloading stress paths (2%−4%) under the same initial confining pressure. The promoting effects of different stress paths on the plastic volumetric deformation of the staggered zone were as follows. The promoting effect of the stress path of unloading axial pressure and unloading confining pressure was the strongest, that of the stress path of constant axial pressure and unloading confining pressure was the second stronger, and that of the stress path of loading axial pressure and unloading confining pressure was the weakest. 2) There were a certain of the dependency of stress path of its internal variables such as plastic volumetric strain, equivalent plastic strain and equivalent plastic work during the deformation and failure process of the staggered zone in different unloading stress paths. Therefore, it is not accurate to directly take any of the above state parameters as the hardening parameters and assume that it is independent of the stress paths in the elastic-plastic analysis of staggered zone. Therefore, a modified method of the equivalent plastic work was proposed. It was found that when the parameter ns reflecting the material properties of staggered zone was equal to -0.4, the modified equivalent plastic work had the obvious independency of stress path, which was more appropriate for describing the unloading plastic strain hardening characteristics of the staggered zone under high in-situ stress unloading conditions. The plastic mechanical characteristics of the staggered zone revealed in this paper can further deepen the understanding of deformation and failure of the staggered zone under high in-situ stress unloading conditions, and provide a theoretical basis for the analysis of the failure mechanism and support control of the staggered zone in practical engineering.

Potentilla anserina L polysaccharide (PAP) possesses many bioactivities. In the present study, molecular weight, monosaccharide composition, FT-IR, molecular morphology of PAPs were characterized, the result showed that Mw of PAP-1, PAP-2, PAP-3, PAP-4, and PAP-5 were 4.480 × 105 Da , 1.284 × 105 Da, 8.377 × 104 Da, 3.639 × 104 Da, 2.412 × 104 Da; PAPs were composed of Rha, Man, Glc and Gal. And then, ROS, MDA, SOD, and GSH-Px were evaluated after radiation damage by γ-rays in RAW264.7 cells. The results demonstrated that PAPs reduce ROS and MDA content, increase the activity of SOD and GSH-Px. Especially, low-molecular weight, high Gal, and low Glu content PAP-4 possessed a better anti-radiation activity. This study provides a scientific basis for the further structure–activity relationship study of PAPs.

Cell phone call operations during driving can lead to distraction and cause potential safety hazards. In order to quantitatively characterize the influence of cell phone call mode on the driver’s visual behavior in different traffic conditions and to analyze the risk level of distracted driving from the visual level, a distracted driving simulation test was carried out based on a driving simulator and eye tracker. The eye movement data of drivers during normal driving, hands-free call, and video call under two typical traffic conditions of free flow and congested flow on the urban expressway were collected. Firstly, four visual characteristics indicators that were highly sensitive to traffic conditions and driving states were selected in terms of visual field range, visual recognition, visual search, and visual load, which were the information entropy of fixation area (IEFA), saccade amplitude, peak-to-average ratio of saccade velocity (PARSV), and relative change intensity of pupil area (RCPA). Then, based on the improved CRITIC method, the visual stability coefficient (VSC) was constructed as a new indicator to comprehensively assess the risk level of the driving state, and the assessment criteria were divided. Finally, the grey correlation analysis method was introduced to verify the assessment effect of VSC. The results show that different cell phone call modes increased driving risk in both traffic conditions. Among them, the negative influence of video calls on driving safety was significantly higher than that of hands-free calls, with a significant decrease in VSC, and the drivers’ VSC in the free flow scenario was more sensitive to the impact of cell phone call operation, and the driving risk increased significantly during distracted driving. The VSC can quantitatively assess driving risk from the perspective of visual psychological safety and contributes to the development of corresponding early warning and control measures.

To explore the effect of snow-melting agents on the glass fiber-reinforced cemented soil under freezing-thawing cycles, three widely used snow-melting agents, including potassium acetate, magnesium chloride, and sodium sulfate, were used in this article. The effects of snow-melting agent types on the apparent damage, mass loss, and mechanical properties of fiber-reinforced cemented soil under freezing-thawing cycles were analyzed through salt freezing and unconfined compressive strength tests. The results show that the snow-melting effect of potassium acetate is the best, the snow-melting effect of magnesium chloride is the second, and the snow-melting effect of sodium sulfate is the worst. Notably, as the number of freezing-thawing cycles increases, the strength of the test block decreases to varying degrees. After the fifth freezing-thawing cycle, the strength of the block without fiber decreased by 61.30%, 70.22%, and 81.58% in clear water, potassium acetate, and magnesium chloride solution, respectively, while the test block in sodium sulfate solution lost its bearing capacity. A series of studies proved that the snow-melting agent with sodium sulfate as the main component has the most apparent erosion effect on the cemented soil, followed by magnesium chloride, and the erosion effect of potassium acetate is the weakest. The incorporation of glass fiber can effectively improve the resistance of the cemented soil under the action of various salt solution erosion and freezing-thawing coupling and has a significant effect on slowing the development of surface cracks, improving peak strength, and reducing the mass loss rate. This research will provide theoretical support for the design of subgrade and the selection of snow-melting agents in cold areas.

Abstract Background Eukaryotic translation initiation factor 6 (eIF6), also known as integrin β4 binding protein, is involved in ribosome formation and mRNA translation, acting as an anti-association factor. It is also essential for the growth and reproduction of cells, including tumor cells. Yet, its role in oral squamous cell carcinoma (OSCC) remains unclear. Methods The expression characteristics of eIF6 in 233 samples were comprehensively analyzed by immunohistochemical staining (IHC). Effects of eIF6 over-expression and knockdown on cell proliferation, migration and invasion were determined by CCK-8, wound healing and Transwell assays. Western blot, immunofluorescence (IF) and co-immunoprecipitation (co-IP) were performed for mechanical verification. Results We found that cytoplasmic eIF6 was abnormally highly expressed in OSCC tissues, and its expression was associated with tumor size and the clinical grade. Amplification of eIF6 promoted the growth, migration and invasion capabilities of OSCC cell lines in vitro and tumor growth in vivo. Through Western blot analysis, we further discovered that eIF6 significantly promotes epithelial-mesenchymal transformation (EMT) in OSCC cells, while depletion of eIF6 can reverse this process. Mechanistically, eIF6 promoted tumor progression by activating the AKT signaling pathway. By performing co-immunoprecipitation, we discovered a direct interaction between endogenous eIF6 and AKT protein in the cytoplasm. Conclusion These results demonstrated that eIF6 could be a new therapeutic target in OSCC, thus providing a new basis for the prognosis of OSCC patients in the future. Video abstract

ObjectiveTo verify the effect of triamcinolone acetonide (TA) and major salivary glands saline irrigation on relieving xerostomia in Sjögren’s syndrome (SS) patients.MethodsThe enrolled 49 SS patients were randomly assigned to the control group (no irrigation, n=16), saline group (irrigation with saline, n=17) and TA group (irrigation with TA, n=16). Fourteen cases of each group were treated differently but received the same examinations. The examinations include unstimulated whole saliva flow (UWS), chewing-stimulated whole saliva flow (SWS), citric acid-stimulated parotid flow (SPF), Clinical Oral Dryness Score (CODS), Xerostomia Inventory (XI) and EULAR SS Patient Reported Index (ESSPRI) of 1 week before irrigation (T0) and 1 week(T1), 8 weeks (T8), 16 weeks (T16) and 24 weeks (T24) after major salivary irrigation.ResultsEach group had 14 cases with completed follow-ups. Both TA and saline irrigation of major salivary glands resulted in higher SWS and SPF of T8, T16 and than those at T0. ESSPRI (oral dryness domain) of T8, T16 and T24 were significantly lower than that at T0, respectively (P < 0.05). SWS and SPF of T8, T16 and T24 in the saline group were significantly higher than in the control group (P< 0.05). XI and ESSPRI (oral dress domain) of T8, T16 and T24 in the saline group were significantly lower than those in the control group, respectively (P< 0.05). SWS and SPF of T16 and T24 in the TA group were significantly higher than in the control group (P< 0.05). All cases with completed follow-up in TA and saline groups were divided into responders and non-responders. Compared with responders, the UWS, SWS, SPF and CODS of T0 in non-responders were significantly increased (P

In order to improve the mechanical properties and deformation characteristics of permeable concrete, glass fiber was added to this type of concrete. Based on an unconfined compressive strength test, non-contact full-field strain measurement system, and scanning electron microscopy test, the effects of aggregate particle composition, shaking time, fly ash content, fiber length, and fiber content on the strength and permeability of permeable concrete were studied. The results show that the strength and water permeability of permeable concrete are negatively correlated with an increase in shaking time. When the aggregate particle size is 5–10 mm, the permeable concrete has both good strength and permeability. Proper incorporation of fly ash improves the compactness inside the structure. The influence of different lengths of glass fiber on the strength of permeable concrete first increases and then decreases, and the permeable property decreases. With the same fiber length, the strength increases first and then decreases with an increase in the content, while the porosity and water permeability coefficient decrease. Under the test conditions, when the length of glass fiber is 6 mm, and the dosage is 2 kg/m3, the strength performance of permeable concrete is the best, and the permeability effect is good at the same time.

Background: The purpose of this study was to compare the effect of pre- and post-treatment furosemide on reducing intravascular absorption syndrome in complex hysteroscopic leiomyoma surgery. Methods: This randomized controlled study enrolled 62 patients who underwent hysteroscopic leiomyoma surgery in Fuxing Hospital, Capital Medical University. Patients were randomly allocated to pre- and post-treatment furosemide groups. Information on patient age, body mass index, sum of the three diameters of the uterus, sum of diameter of submucosal myoma, and intimal thickness were recorded. The mean arterial pressure and heart rate were recorded at entry to the operating room, after anesthesia induction, at the end of operation, and after laryngeal mask pulled out. The duration of anesthesia, recovery time after withdrawal of anesthetic drug, amount of sufentanil, amount of propofol, amount of uterine perfusion fluid, the amount of perfusion fluid flowing out, the actual amount of fluid intake, the proportion of pulmonary diffuse B-line in perioperative period, and uterine perfusion pressure during operation were also recorded, together with postoperative adverse reactions (drowsiness, nausea, vomiting, chills). Results: No significant differences were observed between the pre- and post-treatment groups in terms of patient age, body mass index, sum of the three diameters of the uterus, sum of diameter of submucosal myoma, and intimal thickness. The mean arterial pressure and heart rate at the four different time points were not significantly different between the two groups, nor were there any significant differences in the duration of anesthesia, the amount of sufentanil, the amount of propofol, the amount of intrauterine perfusion fluid, the amount of perfusion fluid flowing out, the actual amount of fluid intake, the intrauterine perfusion pressure, or the incidence of postoperative drowsiness, nausea and vomiting. However, pre-treatment with furosemide was associated with a significantly reduced recovery time (p = 0.037) and incidence of postoperative chills (p = 0.032) compared to the post-treatment group. Furosemide significantly reduces the number of B-lines. Conclusions: Furosemide preconditioning can reduce the recovery time and occurrence of postoperative chills, both of which are conducive to better postoperative recovery. Clinical Trial Registration: The study was registered at https://www.chictr.org.cn (registration number ChiCTR2200062840).

As data relay satellites (DRSs) play an increasingly important supporting role in remote sensing missions, efficient coordination across space–ground multiresources becomes a significant problem. Owing to the implementation problem of the centralized coordinate methods, this paper studies a distributed coordinate resource scheduling method which is realizable in the current space network structure. To be specific, we first formulate the multiple resource coordination problem into an MILP problem based on a modified time-expanded graph. Then, the problem is transferred and decomposed into subproblems for remote sensing satellite (RSS) systems and DRS systems to solve distributedly. Afterwards, we propose a distributed iterative scheme for the RSS systems and DRS systems based on alternating direction method of multipliers (ADMM), in which only the schedule information of the inter-satellite links are required to exchange between RSS systems and DRS systems. Simulation results are provided to validate the effectiveness of our distributed coordinated resource scheduling algorithm.

Abstract Background Asymptomatic infections may play an important role in severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) Delta variant transmissions. However, the immunologic features of asymptomatic postvaccination infections with the Delta variant of SARS‐CoV‐2 in adults remain to be defined. Methods A retrospective study involving 36 vaccinated adults infected with the SARS‐CoV‐2 Delta variant was performed. Their demographic and laboratory data were collected and analyzed in The First People's Hospital of Jingmen from August 4 to 20, 2021. Results Of the 36 adults, 6 persons had an asymptomatic infection. The severity of the SARS‐CoV‐2 infections was highly correlated with the doses of vaccinations (p = 0.019). The symptomatic and asymptomatic infected SARS‐CoV‐2 adults showed normal levels of leukocytes and lymphocytes. The C‐reactive protein (CRP) and interleukin‐6 (IL‐6) levels were elevated in the symptomatic groups. The period between the last vaccination to the time of infection in the asymptomatic group was longer than that in the mild and moderate groups (73 vs. 61 vs. 50 days; p = 0.047). The percentage of suppressor T‐cells in the asymptomatic group was the highest (32.2 ± 4.0% vs. 22.0 ± 7.2% vs. 29.3 ± 8.0%; p = 0.004). The signal‐to‐cutoff ratio value of total antibody against SARS‐CoV‐2 in the asymptomatic group was lower than that in the other two groups (383 vs. 703 vs. 1792; p

The effect of expanded body diameter on the displacement field of soil surrounding a pile under different vertical loads was investigated using the half-face pile model test of undisturbed soil. Digital image correlation technology was used to record the displacement characteristics of soil around the pile in real time. The displacement and failure characteristics of the soil around the pile were analyzed. The results show that with an increased load, the soil below the expanded body is compressed, and the soil at both ends will slip, leading to the continuous development of cracks. In a horizontal direction, the soil surrounding the pile first moves close to the pile and then tends to stabilize or move away from the pile. The horizontal and vertical displacement of the soil decreases as the distance from the pile increases. The main area of influence on the soil is below the expanded body, in which the increased diameter of the expanded body results in a gradual increase in the area of influence. Furthermore, all of the load-settlement curves show a slow decline and the bearing capacity increases with the increased diameter of the expanded body. Therefore, the research in this paper can provide an experimental method for the study of soil displacement around drill-expanded concrete piles.

Abstract Background HIIT has recently been widely used for health promotion in healthy people and patients with chronic diseases. Exercise can help SUD reduce drug cravings, enhance mental health and return to normal life. However, whether HIIT can bring better physical rehabilitation benefits to individuals with SUD than MICT is unclear. The study aimed to compare the effects of HIIT versus MICT on the physical fitness of individuals with SUD. Methods One hundred twenty individuals with amphetamine-type stimulant dependence voluntarily participated in this study. They were randomly assigned to the HIIT group and MICT group. Both groups received training three times a week. The intervention lasted from January 2019 to December 2019. Physical fitness was assessed at the baseline, 3 months, 6 months, 9 months and 12 months, including blood pressure (BP), vital capacity(VC), hand grip, push-up, sit-and-reach, one-leg standing with eyes closed and choice reaction time. The craving level was assessed using the Visual Analog Scale at baseline, 6 months and 12 months to see any change along with the improvement in physical fitness. Two-way repeated measures ANOVA was applied to analyse the differences in change by group (HIIT and MICT) and time (baseline, 3 months, 6 months, 9 months and 12 months). Results The within-group factor displayed significant changes in the HIIT and MICT groups in terms of systolic BP (F (4,336) = 12.799, P

Under endoplasmic reticulum (ER) stress, tumor plays multifaceted roles in endothelial cell dysfunction through secreting exosomal miRNAs. However, for the head and neck squamous cell carcinoma (HNSCC), it is still unclear about the impact of ER-stressed HNSCC cell derived exosomes on vascular endothelial cells. To address this gap, herein, systemic research was conducted including isolation and characterization of ER-stressed HNSCC cell (HN4 cell line as an in vitro model) derived exosomes, identification of regulatory exosomal miRNAs, target exploration and downstream signaling pathway investigation of exosomal miRNAs in human umbilical vein endothelial cell (HUVEC). ER-stressed HN4 cell-derived exosomes inhibited angiogenesis and migration of HUVEC cells in vitro . Furthermore, RNA-seq analysis demonstrated that miR-424-5p was highly upregulated in ER-stressed HN4 cell-derived exosomes. Through matrigel tube formation and transwell assays of HUVEC cells, miR-424-5p displayed great capabilities on inhibiting angiogenesis and migration. Finally, based on western blot and luciferase reporter, it was demonstrated that LAMC1 is the target of miR-424-5p which could inhibit the angiogenesis and migration of HUVEC cells by repressing the LAMC1-mediated Wnt/β-catenin signaling pathway. ER-stressed HNSCC cell-induced exosomal miR-424-5p inhibits angiogenesis and migration of HUVEC cells through LAMC1-mediated Wnt/β-catenin signaling pathway. This study offers a new insight for understanding the complicated mechanism behind ER-stress induced anti-angiogenesis of HNSCC.

Abstract Purpose The purpose of the systematic review and meta-analysis is to analyze the application value of the stoma support rods in loop enterostomy. Methods The studies on the application of stoma rods in loop enterostomy published from January 2000 to January 2020 were searched in the databases of PubMed, Embase, Cochrane library, and Clinical trials. All randomized controlled trials (RCTs) and cohort studies that observed the value of stoma rods were included according to inclusion criteria. The RevMan5.3 software was used for statistical analysis. Results A total of 1131 patients with loop enterostomy in six studies were included in this study; there were 569 cases in the experimental group and 562 cases in the control group. All six studies analyzed the effect of support rods on the incidence of stoma retraction; the meta-analysis showed that in a total of 32 patients, stoma retraction occurred, with a total incidence of about 2.8% in 1131 patients. The incidence of stoma retraction in the rod group was not significantly lower than that in the non-rod group, and the difference was not statistically significant (OR = 0.65, 95% CI 0.32~1.32, I 2 = 0%, P = 0.23), and the studies were homogeneous. The incidences of stoma necrosis (OR = 6.41, 95% CI 2.22~18.55, I 2 = 0%, P = 0.0006), peristomal dermatitis (OR = 2.93, 95% CI 2.01~4.27, I 2 = 0%, P < 0.00001), and mucocutaneous separation (OR = 2.14, 95% CI 1.03~4.47, I 2 = 0%, P = 0.04) were significantly increased in the rod group. Conclusions It is not recommended to routinely use stoma support rods in the clinical practice.

Abstract Background MicroRNAs (miRNAs) play a role in regulating osteogenic differentiation (OD) of mesenchymal stem cells by inhibiting mRNAs translation under cyclic strain. miR-503-3p was downregulated in OD of human adipose-derived stem cells (hASCs) in vivo under cyclic strain in our previous study, while it might target the Wnt/β-catenin (W-β) pathway. In this study, we explored miR-503-3p’s role in OD of hASCs under cyclic strain. Methods OD of hASCs was induced by cyclic strain. Bioinformatic and dual luciferase analyses were used to confirm the relationship between Wnt2/Wnt7b and miR-503-3p. Immunofluorescence was used to detect the effect of miR-503-3p on Wnt2/Wnt7b and β-catenin in hASCs transfected with miR-503-3p mimic and inhibitor. Mimic, inhibitor, and small interfering RNA (siRNA) transfected in hASCs to against Wnt2 and Wnt7b. Quantitative real-time PCR (RT-PCR) and western blot were used to examine the OD and W-β pathway at the mRNA and protein levels, respectively. Immunofluorescence was performed to locate β-catenin. ALP activity and calcium were detected by colorimetric assay. Results Results of immunophenotypes by flow cytometry and multi-lineage potential confirmed that the cultured cells were hASCs. Results of luciferase reporter assay indicated that miR-503-3p could regulate the expression levels of Wnt2 and Wnt7b by targeting their respective 3′-untranslated region (UTR). Under cyclic strain, gain- or loss-function of miR-503-3p studies by mimic and inhibitor revealed that decreasing expression of miR-503-3p could significantly bring about promotion of OD of hASCs, whereas increased expression of miR-503-3p inhibited OD. Furthermore, miR-503-3p high-expression reduced the activity of the W-β pathway, as indicated by lowering expression of Wnt2 and Wnt7b, inactive β-catenin in miR-503-3p-treated hASCs. By contrast, miR-503-3p inhibition activated the W-β pathway. Conclusions Collectively, our findings indicate that miR-503-3p is a negative factor in regulating W-β pathway by Wnt2 and Wnt7b, which inhibit the OD of hASCs under cyclic strain.

Abstract Background Opsoclonus-myoclonus syndrome (OMS) is a rare neurological disease. Some children with OMS also have neuroblastoma (NB). We and others have previously documented that serum IgG from children with OMS and NB induces neuronal cytolysis and activates several signaling pathways. However, the mechanisms underlying OMS remain unclear. Here, we investigated whether nitric oxide (NO) from activated microglias and its cascade contribute to neuronal cytolysis in pediatric OMS. Methods The activation of cultured cerebral cortical and cerebellar microglias incubated with sera or IgG isolated from sera of children with OMS and NB was measured by the expression of the activation marker, cytokines, and NO. Neuronal cytolysis was determined after exposing to IgG-treated microglia-conditioned media. Using inhibitors and activators, the effects of NO synthesis and its intracellular cascade, namely soluble guanylyl cyclase (sGC) and protein kinase G (PKG), on neuronal cytolysis were evaluated. Results Incubation with sera or IgG from children with OMS and NB increased the activation of cerebral cortical and cerebellar microglias, but not the activation of astrocytes or the cytolysis of glial cells. Moreover, the cytolysis of neurons was elevated by conditioned media from microglias incubated with IgG from children with OMS and NB. Furthermore, the expression of NO, sGC, and PKG was increased. Neuronal cytolysis was relieved by the inhibitors of NO signaling, while neuronal cytolysis was exacerbated by the activators of NO signaling but not proinflammatory cytokines. The cytolysis of neurons was suppressed by pretreatment with the microglial inhibitor minocycline, a clinically tested drug. Finally, increased microglial activation did not depend on the Fab fragment of serum IgG. Conclusions Serum IgG from children with OMS and NB potentiates microglial activation, which induces neuronal cytolysis through the NO/sGC/PKG pathway, suggesting an applicability of microglial inhibitor as a therapeutic candidate.

For the security transmission of artificial noise with finite alphabet inputs in secure spatial modulation system,an antenna selection algorithm based on cut-off rate (COR) was proposed.Considering that it was difficult to obtain the closed-form expression of the secrecy rate,the problem of maximizing the secrecy rate into that of maximizing the COR was converted by the algorithm.Meanwhile,in order to reduce the complexity,the COR algorithm was optimized by expanding the matrix norm in columns,which was corresponding to the problem of antenna combination.Finally,the artificial noise vector was mapped in the legitimate channel space to optimize secrecy rate,which only interferes with eavesdropper.The simulation results show that the proposed algorithm based on COR is superior to the leakage-based algorithm and the maximum secrecy rate algorithm,and has lower complexity.

White celery (Apium graveolens L.), a variety of common celery, is an important leafy vegetable in the Apiaceae family and is famous for its nutritional value. However, limited work has been devoted to quality formation and regulation in white celery. In this study, four white celery varieties, ‘Xuebaiqincai’, ‘Saixue’, ‘Baiganshiqin’ and ‘Ruixue’, were selected and analyzed for the comparison of ascorbic acid (AsA) and lignin levels, which are two important quality evaluation indicators in celery. The expression levels of the genes involved in AsA and lignin metabolic pathways were also detected. In the leaf blades, the AsA content was highest in ‘Xuebaiqincai’ compared with other varieties, whereas the most abundant AsA levels in the petioles were observed in ‘Baiganshiqin’ and ‘Ruixue’. The expression levels of AsA-related genes varied among the studied varieties. The highest level was detected in ‘Xuebaiqincai’, whereas other varieties exhibited relatively lower levels. The lignin content in the leaf blades was lower than that in the petioles. Correspondingly, the transcript profiles of genes involved in lignin biosynthesis were in accordance with the different levels in the petioles and blades. The results of this study provide potentially useful information for white celery breeding aimed at quality improvement and regulation.

Due to the advantage of high data rate, low transmission delay and high reliability, the application of Long Term Evolution for Vehicle (LTE-V) has got more attention on Vehicular Ad-hoc Networks (VANETs). However, the fixed Road Side Units (RSUs), like Base Stations (BSs), in LTE-V have a small coverage, which need consume a large amount of energy to achieve long-distance communication. This could limit the LTE-V application in some practical situations. In this paper, we introduce Energy Harvest Road Side Units (EH-RSUs) in some low frequency service area instead of fixed-point RSUs (BSs) to reduce the cost of deployment and maintenance. Different from fixed-point RSUs with the wired electricity sources, EH-RSUs are powered by themselves and the service time is affected by battery capacity, charging speed, service radius and communication load, which need to be considered comprehensively. To solve these problems, we construct an EH-RSUs deployment model framework based on communication load conditions. Then, on the basis of this framework, we propose an optimization problem to minimize the deployment and operation cost of EH-RSUs and fixed-point RSUs, where the service radius of the EH-RSUs are taken as an optimization variable. Finally, a pre-deployment algorithm is proposed to solve the optimization problem. Simulations evaluate the validity of our proposed method. The results show that the energy consumption with the proposed method could be reduced to 60% compared with only fixed-point RSUs deployed.

Objectives: Hysteroscopic surgery, as a minimally invasive gynecological diagnosis and treatment technique, has advantages including less trauma, quick recovery, and short hospital stay that are very common in clinical practice; however, certain problems remain. Using large amounts of distending medium can induce a series of body changes such as diluent hyponatremia, pulmonary edema and hypothermia. According to the 2021 Chinese Expert Consensus on anesthesia management for hysteroscopic diagnosis and treatment, the most common complication of hysteroscopic surgery was uterine perforation (0.12%), followed by operative hysteroscopy intravascular absorption syndrome (OHIA) (0.06%), intraoperative bleeding (0.03%), air embolism syndrome (0.03%), bladder or bowel injury (0.02%), and endometritis (0.01%). The incidence of uterine perforation and endovascular absorption syndrome was higher than others. With advances in both medical technology and devices, the incidence of uterine perforation has gradually decreased. Severe adhesions, multiple endometrial polyps, large uterine fibroids, etc., increase the difficulty of surgery, thus increasing operation time and the amount of fluid perfusion during surgery. OHIA has become the most important concern of clinicians during hysteroscopic surgery. At present, the prevention and treatment of OHIA remains to be further studied; accordingly, this article seeks to provide a review of current treatment modalities of OHIA during hysteroscopic surgery. Mechanism: Medline, Web of Science, and Ovid databases were searched using the following terms: distending medium, operative hysteroscopy, intravascular absorption syndrome; complications of hysteroscopic surgery. Findings in Brief: Shortening the operation time, reducing the volume of distending medium, decreasing infusion pressure, and using a bipolar scope can reduce the occurrence of OHIA during hysteroscopic surgery. Conclusions: Effective means of controlling and limiting OHIA during hysteroscopic surgery include: exploring the appropriate temperature of perfusion fluid; mastering the advanced monitoring methods; promoting the advanced surgical energy and perfusion system; and improving the surgical team’s understanding, diagnosis and treatment level of hysteroscopic surgical complications for the safety of perioperative patients’ vital signs.