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Thaumatin-like proteins (TLPs) in plants are involved in diverse biotic and abiotic stresses, including antifungal activity, low temperature, drought, and high salinity. However, the roles of the TLP genes are rarely reported in early flowering. Here, the TLP gene family was identified in P. trichocarpa. The 49 PtTLP genes were classified into 10 clusters, and gene structures, conserved motifs, and expression patterns were analyzed in these PtTLP genes. Among 49 PtTLP genes, the PtTLP6 transcription level is preferentially high in stems, and GUS staining signals were mainly detected in the phloem tissues of the PtTLP6pro::GUS transgenic poplars. We generated transgenic Arabidopsis plants overexpressing the PtTLP6 gene, and its overexpression lines showed early flowering phenotypes. However, the expression levels of main flowering regulating genes were not significantly altered in these PtTLP6-overexpressing plants. Our data further showed that overexpression of the PtTLP6 gene led to a reactive oxygen species (ROS) burst in Arabidopsis, which might advance the development process of transgenic plants. In addition, subcellular localization of PtTLP6-fused green fluorescent protein (GFP) was in peroxisome, as suggested by tobacco leaf transient transformation. Overall, this work provides a comprehensive analysis of the TLP gene family in Populus and an insight into the role of TLPs in woody plants. [ABSTRACT FROM AUTHOR]

Many emerging markets rely on land financing, whereby land grants are used to raise funds for the government. In the short term, land financing eases the government's fiscal deficit and boosts regional economic development. However, the long-term implications of such behaviour have not been adequately discussed. This study focuses on the relationship between local government land finance dependence (LGLFD) and urban innovation quality (UIQ). We find that LGLFD significantly inhibits the improvement of UIQ, and this inhibition occurs through three main channels: changing government spending preferences, reducing financial efficiency, and deteriorating the institutional environment. Our empirical study analyses 3662 samples from 264 Chinese cities from 2003 to 2016, confirming our research hypothesis. Further research finds that there is significant heterogeneity in the effect of LGLFD on UIQ. Based on these conclusions, some policy implications are proposed. [ABSTRACT FROM AUTHOR]

China has been the world's largest producer and consumer of paper products. In the context of the "carbon peaking and carbon neutrality goals", China's papermaking industry which is traditionally a high energy-consuming and high-emissions industry, desperately needs a nationally appropriate low-carbon development path. From the consumption-side perspective, this paper calculates the CO2 emissions of China's papermaking industry from 2000 to 2019 by using carbon emission nuclear algorithm, grain-straw ratio, first-order attenuation method, and STIRFDT decomposition model, etc., to further explore the core stages and basic patterns affecting the industry's carbon peaking. The results show that the total CO2 emissions of China's papermaking industry showed an upward trend from 2000–2013, stable from 2013–2017, and a steady but slight decline from 2017–2019. Meanwhile, the total CO2 emissions of the full life cycle of paper products in China have decreased to a certain extent in the raw material acquisition, pulp, and paper making and shipping stages, with only the waste paper disposal stage showing a particular upward trend. We find that from 2000 to 2019, China's CO2 emissions in the pulping and papermaking stage of paper products accounted for 68% of the total emissions in the whole life cycle, of which 59% was caused by coal consumption. Moreover, the scenario prediction shows that improving the energy structure and increasing the waste paper recovery rate can reduce the CO2 emissions of the industry, and it is more significant when both work. Based on this and the four core stages of CO2 emissions of the papermaking industry we proposed ways to promote CO2 emissions peaking of China's paper products. [ABSTRACT FROM AUTHOR]