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Introduction: This study aimed to investigate the effects of a low-fishmeal diet (LF, substituting soybean meal for 40% fish meal) and the supplementation of 500 mg/kg and 1000 mg/kg Yu Ping Feng (YPF) polysaccharides on the growth performance, antioxidant enzyme activities, intestinal ultrastructure, non-specific immunity, and microbiota of Macrobrachium rosenbergii. Methods: The study involved the administration of different diets to M. rosenbergii, including a control diet, a low-fishmeal diet (LF), and LF diets supplemented with 500 mg/kg and 1000 mg/kg YPF polysaccharides. Growth performance, antioxidant enzyme activities, intestinal ultrastructure, non-specific immunity, and microbiota were assessed. Results: The LF diet significantly reduced growth performance parameters compared to the control group. However, YPF supplementation notably improved these parameters, with the greatest improvement observed at a 1000 mg/kg dosage. Antioxidant enzyme activities (SOD, GSH-PX) were diminished in the LF group, accompanied by elevated MDA levels, whereas YPF supplementation restored these activities and reduced MDA levels. Ultrastructural analysis revealed that the LF diet caused intestinal villi detachment and peritrophic matrix (PM) shedding, which were alleviated by YPF. Gene expression related to PM formation (GS, CHS, EcPT) was downregulated in the LF group but significantly upregulated in the 1000P group. Non-specific immune gene expressions (IMD, Relish, IκBα) and enzyme activities (NO, iNOS) were suppressed in the LF group but enhanced by YPF supplementation. Microbial community analysis showed reduced diversity and altered composition in the LF group, with increased Proteobacteria and decreased Firmicutes, which were partially restored by YPF. Correlation analysis revealed that Lactobacillus and Chitinibacter play pivotal roles in regulating intestinal health. Lactobacillus exhibited a positive relationship with the intestinal PM and immune-related indicators, whereas Chitinibacter was negatively associated with these factors. Discussion: These results highlight the adverse impacts of a low-fishmeal diet on the intestinal health of M. rosenbergii and demonstrate the beneficial effects of YPF polysaccharides in alleviating these negative consequences through various mechanisms, including improved growth performance, enhanced antioxidant enzyme activities, restored intestinal ultrastructure, and modulated immune responses. The findings suggest that YPF supplementation could be a valuable strategy for mitigating the negative effects of low-fishmeal diets in aaquaculture. [ABSTRACT FROM AUTHOR]

Large-scale intensive feeding triggered reduced growth performance and nutritional value. Exogenous probiotics can promote the growth performance and nutritional value of fish through improving the intestinal microbiota. However, detailed research on the correlation between the intestinal microbiota, growth performance, and nutritional value remains to be elucidated. Therefore, we performed metagenomic and metabolomic analysis to investigate the effects of probiotic addition to basal diet (1.0 × 108 CFU/g) (PF) and water (1.0 × 108 CFU/g) (PW) on the growth performance, muscle nutritional value, intestinal microbiota and their metabolites, and glucolipid metabolism in Coilia nasus. The results showed that FBW, BL, and SGR were enhanced in PF and PW groups. The concentrations of EAAs, TAAs, SFAs, MUFAs, and PUFAs were increased in PF and PW groups. Metagenomic and metabolic analyses revealed that bacterial community structure and metabolism were changed in the PF and PW groups. Moreover, adding probiotics to diet and water increased SCFAs and bile acids in the intestine. The gene expression associated with lipolysis and oxidation (hsl, pparα, cpt1, and acadm) and glycolysis (gck and pfk) was upregulated, while the gene expression associated with lipid synthesis (srebp1, acc, dgat, and elovl6) and gluconeogenesis (g6pca1, g6pca2, and pck) was downregulated in the liver. Correlation analysis displayed that hepatic glucolipid metabolism was regulated through the microbiota–gut–liver axis. Mantel test analysis showed that growth performance and muscle nutritional value were improved by the gut–liver axis. Our findings offered novel insights into the mechanisms that underlie the enhancement of growth performance and nutritional value in C. nasus and other fish by adding probiotics. [ABSTRACT FROM AUTHOR]

Salinity is a significant environmental component that affects the physiological state of aquatic species. This study aimed to investigate whether water salinity had an impact on the biochemical properties and meat quality of adult Aplodinotus grunniens during temporary rearing of 7 days. Salinity caused increased osmotic pressure and antioxidant enzyme activities of Aplodinotus grunniens, which were attributed to the increase in the content of alanine and glutamate. It raised the hardness and shear force with an increase in salinity, leading to an increase in water-holding capacity. Salinity enhanced the DHA ratio with a decrease in the atherosclerotic index and thrombosis index. Combined with the increase in flavor amino acids and nucleotides, salinity enhanced the umami taste of Aplodinotus grunniens. These findings suggest that temporary rearing in salinity might be a practical approach to improving the meat quality of adult Aplodinotus grunniens. [ABSTRACT FROM AUTHOR]

Protein-based emulsion gels are an ideal delivery system due to their unique structure, remarkable encapsulation efficiency, and tunable digestive behavior. Freshwater mussel (Solenaia oleivora) protein isolate (SoPI), an emerging sustainable protein with high nutritional value, possesses unique value in the development of functional foods. Herein, composite emulsion gels were fabricated with SoPI and κ-carrageenan (κ-CG) for the delivery of curcumin. SoPI/κ-CG stabilized emulsions possessed a high encapsulation efficiency of curcumin with a value of around 95%. The addition of κ-CG above 0.50% facilitated the emulsion gel formation and significantly improved the gel strength with 1326 g. Furthermore, the storage and digestive stability of curcumin were significantly improved as the κ-CG concentration increased. At 1.50% κ-CG, around 80% and 90% curcumin remained after 21-day storage at 45 °C and the 6 h in vitro gastrointestinal digestion, respectively. The addition of 0.50% κ-CG obtained the highest bioaccessibility of curcumin (~60%). This study illustrated the potential of SoPI emulsion gels as a carrier for stabilizing and delivering hydrophobic polyphenols. [ABSTRACT FROM AUTHOR]