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The potential application of high aspect-ratio nanomaterials motivates the development of the fabrication and modification of lipid nanotubes(LNTs). To date, diverse fabricate processes and elaborate template procedures have produced suitable tubular architectures with definite dimensions and complex structures for expected functions and applications. Herein, we comprehensively summarize the fabrication of LNTs in vitro and discuss the progress made on the micro/nanomaterials fabrication using LNTs as a template, as well as the functions and possible application of a wide range of LNTs as fundamental or derivative material. In addition, the characteristics, advantages, and disadvantages of different fabrication, modification methods, and development prospects of LNTs were briefly summarized. [Display omitted] • Fabrication, modification of LNTs and the potential applications on biological, physical and chemical field. [ABSTRACT FROM AUTHOR]