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22. Additional file 1 of Bone-targeting delivery of platelet lysate exosomes ameliorates glucocorticoid-induced osteoporosis by enhancing bone-vessel coupling

Author

Zheng, Gang, Ma, Hai-Wei, Xiang, Guang-Heng, He, Gao-Lu, Cai, Han-Chen, Dai, Zi-Han, Chen, Yan-Lin, Lin, Yan, Xu, Hua-Zi, Ni, Wen-Fei, Xu, Cong, Liu, Hai-Xiao, and Wang, Xiang-Yang

Abstract

Additional file 1: Figure S1. FTIR spectra assay of ALN, DSPE-PEG-NHS and DSPE-PEG-ALN. A FTIR spectra result of ALN. B FTIR spectra result of DSPE-PEG-NHS. The intensity of peak at 1,733 cm-1, correspondent to C=O stretch of NHS groups. C FTIR spectra result of DSPE-PEG-ALN. The characteristic peak of NHS groups is strongly reduced in the DSPE-PEG-ALN, which is consistent with nucleophilic substitution of NHS for alendronate. Figure S2. 1H-NMR spectroscopy result of ALN. A Chemical structure of ALN. B 1H-NMR results of ALN in the range of 1.2ppm to 3.2 ppm. The characteristic signal at 1.88 and 2.93 ppm corresponding to CH2 protons. Figure S3. 1H-NMR spectroscopy result of DSPE-PEG-NHS. A Chemical structure of DSPE-PEG-NHS. B 1H-NMR results of DSPE-PEG-NHS in the range of 0 ppm to 10 ppm, evidencing the main peak of PEG chain (3.64 ppm). C 1H-NMR results of DSPE-PEG-NHS in the range of 0 ppm to 4.6 ppm, evidencing the carbon lateral chains (1.25 ppm), terminal methyl groups (0.80 ppm) and NHS (2.67 ppm, arrow). Figure S4. 1H-NMR spectroscopy result of DSPE-PEG-ALN.A Chemical structure of DSPE-PEG-ALN. B 1H-NMR results of DSPE-PEG-ALN in the range of 0 ppm to 10 ppm, evidencing the main peak of PEG chain (3.64 ppm). C1H-NMR results of DSPE-PEG-ALN in the range of 0 ppm to 3.7 ppm showed a reduction of NHS group signal (2.67 ppm) and the appearance of ALN characteristic signal (1.98 and 3.13 ppm). Figure S5. H&E staining of heart, liver, spleen, lung and kidney in five groups. Figure S6. A Zeta potential value of PL, PL-exo and PL-exo-ALN. B The cell viability of BMSCs treated as above was detected by CCK-8. C H&E staining of liver in the above groups. D The osteogenesis- and angiogenesis-associated growth factors in PL and PL-exo was determined by ELISA. All results are presented as the means ± SDs, *P < 0.05, **P < 0.01. Figure S7. Effects of PL-exo on osteogenic and angiogenic differentiation of BMSCs and EPCs. A–C Early osteogenic differentiation was determined by ALP staining and ALP activity assays after 5 days of induction. Late osteogenic differentiation was determined by Alizarin Red staining and the calcium deposition was quantified by measuring the optical density, after 14 days of induction. D–E In vitro tube formation assay of EPCs treated as indicated (scale bar: 150 μm). F–G The expression levels of p-AKT, AKT, Col1 and β-catenin in BMSCs treated as indicated for 36 h. Exosomes (50 µg/mL) and MK-2206 (5μM, a selective inhibitor of AKT) were added to the related medium in the experimental groups, while the control group was cultured in osteogenic induction medium without any addition. H–I The expression levels of p-AKT, AKT, VEGF and Hif-α in EPCs treated as indicated for 24 h. Exosomes (50 µg/mL) and MK-2206 (5μM) were added to the related medium in the experimental groups, while the control group was cultured in EGM-2 complete medium without any addition. All results are presented as the means ± SDs, *P < 0.05, **P < 0.01. Figure S8. Effects of PL, PL-exo and PL-exo-ALN on RANKL-induced osteoclastogenesis in BMMs. A Representative images of TRAcP-stained osteoclasts after treated with PL, PL-exo and PL-exo-ALN. B The quantitative analysis of TRAcP-positive multinucleated cells (>3 nuclei) per well (96-well plate). All results are presented as the means ± SDs, **P < 0.01. Table S1. Post hoc power analyses of the different experiments (Unpaired t-tests). Table S2. Post hoc power analyses of the different experiments (One-way ANOVA).

Published
2022
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43. Wright's Technique with the Addition of Visualized Axial Cortical Windows in Odontoid Fractures.

Author

Wang, Ben, Jin, Jie, Shao, Zhen‐xuan, Yang, Guang‐yong, Lin, Yan, Xu, Hua‐zi, Xie, Cheng‐long, Chen, Jiao‐xiang, Zhang, Xiao‐lei, Hong, Zheng‐hua, and Wang, Xiang‐yang

Subjects
NECK pain, FLUOROSCOPY, BONE screws, LENGTH of stay in hospitals, SPINAL canal, BODY mass index, SPINAL implants
Abstract

This study sought to investigate and evaluate a modified axial translaminar screw fixation for treating odontoid fractures. We performed a retrospective study at Wenzhou Medical University Affiliated Second Hospital between March 2016 and June 2018. We retrospectively collected and analyzed the medical records of 23 cases with odontoid fractures. All patients were identified as type II odontoid fractures without neurological deficiency and serious diseases following the classification of Anderson. The average age, gender ratio, and body mass index (BMI) were 54.3 ± 11.1 years, 12 men to 11 women, and 22.6 ± 2.4 kg/m2, respectively. Patients in this study accepted screw fixation using our modified axial translaminar screw fixation combined with atlas pedicle or lateral mass screw fixation. Within the technique, a small cortical "window" was dug in the middle of the axial contralateral lamina, such that the screws in the lamina were visualized to prevent incorrectly implanting the posterior spinal canal through the visualized "window." A total of 46 bone screws were accurately inserted into the axial lamina without using fluoroscopy. The length of all translaminar screws ranged between 26 and 30 mm, while the diameter was 3.5 mm. During the follow‐up survey, the visual analog scale (VAS) and neck disability index (NDI) were measured. We provide a simple modification of Wright's elegant technique with the addition of "visualized windows" at the middle of the axial lamina. In all patients, screws were inserted accurately without bony breach and the screw angle was 56.1 ± 3.0°. Mean operative time was 102 ± 28 min with an average blood loss of 50 ± 25 mL. Postoperative hemoglobin and mean length of hospital stay were 12.0 ± 1.4 g/dL and 10.4 ± 3.4 days, respectively. The average follow‐up time of all cases was 14.7 months and no internal fixation displacement, loosening, or breakage was found. All patients with odontoid fractures reported being satisfied with the treatment during the recheck period and good clinical outcomes were observed. At 1, 6, and 12 months, NDI and VAS showed that the symptoms of neck pain and limitations of functional disability improved significantly during follow‐up. Our results suggest that the modified translaminar screw fixation technique can efficiently treat Anderson type II odontoid fracture, followed by the benefits of less soft tissue dissection, simple operation, no fluoroscopy, and accurate placement of screws. [ABSTRACT FROM AUTHOR]

Published
2022
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50. Erratum to: Regulation of Caveolin-1 and Junction Proteins by bFGF Contributes to the Integrity of Blood–Spinal Cord Barrier and Functional Recovery

Author

Ye, Li-Bing, primary, Yu, Xi-Chong, additional, Xia, Qing-Hai, additional, Yang, Ying, additional, Chen, Da-Qing, additional, Wu, Fenzan, additional, Wei, Xiao-Jie, additional, Zhang, Xie, additional, Zheng, Bin-Bin, additional, Fu, Xiao-Bing, additional, Xu, Hua-Zi, additional, Li, Xiao-kun, additional, Xiao, Jian, additional, and Zhang, Hong-Yu, additional

Published
2016
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