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Ming-fan Ye,1,* Dong Lin,2,* Wu-jin Li,1 Hai-peng Xu,2 Jing Zhang2 1Department of Chest Surgery, Fujian Provincial Hospital, Fuzhou, Fujian Province, People’s Republic of China; 2Department of Thoracic Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing Zhang Tel +86 13809557405Email fjzlyy123@163.comPurpose: Non-small cell lung cancer (NSCLC) accounts for approximately 80– 85% of all lung cancers, with the FAS-associated factor 1 (FAF1) acting as a tumor suppressor. MicroRNAs (miRNAs) can influence cancer progression by targeting oncogenes or anti-oncogenes. In this study, we aimed to reveal the influence of miR-26a-5p on the regulation of FAF1 expression and NSCLC progression, with the motivation of identifying a potential therapeutic target for NSCLC treatment.Methods: A dual-luciferase reporter assay was used to check for the direct targeting of FAF1 by miR-26a-3p. The miR-26a-5p inhibitor or FAF1 shRNA plasmid was transfected into A549 and H1299 cells to modulate FAF1 expression. Then, the effect of miR-26a-5p/FAF1 on cellular functions was investigated. MTT assay was used to evaluate cell viability. EdU proliferation assay and cell cycle assay were performed to analyze the effect of miR-26a-5p on cell replication and cell cycle. We used annexin V-FITC and PI to stain apoptotic cells, followed by flow cytometric analysis. Transwell and wound healing assays were performed to investigate metastasis. Moreover, the effect of miR-26a-5p/FAF1 on cancer progression was examined in vivo. Lastly, the underlying mechanism was uncovered using RT-qPCR, Western blotting, and TOP/FOP flash assay.Results: miR-26a-5p was found to directly target FAF1 and downregulate its expression. Blocking miR-26a-5p inhibited the cell growth, migration, and invasion, but promoted cell apoptosis. In addition, this inhibited the growth of tumor in mice. FAF1 knockdown reversed the functions of miR-26a-5p. Further, miR-26a-5p/FAF1 was observed to play an important role in the Wnt signaling pathway, regulating the expression of genes such as AXIN, c-Myc, and cyclin-D1.Conclusion: Taken together, we show that miR-26a-5p functions as an oncogenic microRNA in NSCLC by targeting FAF1 and may serve as a potential target for NSCLC treatment.Keywords: miR-26a-5p, FAF1, NSCLC, proliferation, apoptosis, metastasis

Permian strata containing abundant brachiopods are well developed in the Himalaya Tethys Zone. However, relatively few has been systematically described due to the difficult working condition for collecting. In this paper, we describe the brachiopods from the Qubuerga Formation at the Tulong and Kujianla sections in southern Tibet. The brachiopod faunas consist of 15 species belonging to 11 genera. Among the identified 15 species, Retimarginfera xizangensis, Costiferina indica, Fusispirifer semiplicatus, Spiriferella sinica, Biplatyconcha grandis and Neospirifer (Quadrospina) tibetensis are very common in the equivalents of the Himalaya Tethys Zone including the Selong Group at the Selong Xishan and Qubu sections in southern Tibet, the Senja Formation in northwest Nepal, the Zewan Formation in Kashmir, and the upper part of the Wargal Formation and the Chhidru Formation in the Salt Range, Pakistan. They are all comparable and can be assigned to the Wuchiapingian- early Changhsingian. Since the brachiopods from the Qubuerga Formation at Tulong and Kujianla are all composed of typical Gondwanan, bipolar or cosmopolitan elements, it is conclusive that the Himalaya Tethys Zone in the northern margin of the Indian Plate was still situated at southern high-latitudes under cold palaeoclimatic conditions during most of the Lopingian. The faunal succession at Tulong also recorded a rapid warming at the very end of the Changhsingian in view of the fact that the typical cold-water Lopingian brachiopod, gastropod and bivalve faunas were dramatically replaced by extremely abundant conodonts Clarkina in the basal part of the dolostone unit of the Tulong Formation. This end-Changhsingian warming is comparable with that recorded throughout the Permian-Triassic boundary interval at Selong, Qubu in southern Tibet, the Salt Range, Pakistan and the sections in Kashmir as well as South China., Rivista Italiana di Paleontologia e Stratigrafia (Research In Paleontology and Stratigraphy), V. 124, N. 1 (2018)

Summary of the supplementary informarion of reciprocal activation between STAT3 and miR-181b regulates the proliferation of esophageal cancer stem-like cells via the CYLD pathway.(DOC 255 kb)