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Background: The prevalence of peripheral artery disease (PAD) is obviously increased in patients with diabetes. Existing evidence shows that cysteine-rich angiogenic inducer 61 (Cyr61), a 40-kD secreted protein, plays important roles in regulating cellular physiological processes. Recent studies have demonstrated a significant correlation between serum Cyr61 and atherosclerosis. However, the relationship between Cyr61 levels and PAD in patients with type 2 diabetes (T2DM) remains obscure. Methods: Data from a total of 306 subjects with T2DM were cross-sectionally analysed. The extent of PAD was determined by using the Fontaine classification, which defines four stages. We measured serum Cyr61 concentrations by ELISA in subjects with and without PAD at Fontaine's stage II, III, or IV. Logistic regression models were used to examine the independent association of Cyr61 with PAD. Results: Out of the 306 subjects enrolled, 150 were free from PAD, while 156 had clinically significant PAD. In subjects with PAD, the prevalences of Fontaine classification stages II, III and IV were 48.7%, 32.1%, and 19.2%, respectively. Patients with more advanced PAD had significantly higher Cyr61 (P for trend < 0.001). The prevalence of PAD on the basis of severity increased with increasing Cyr61 quartiles (all P values for trends < 0.001), and the severity of PAD was positively correlated with Cyr61 quartiles (r = 0.227, P = 0.006). The association of Cyr61 levels with PAD remained after adjusting for major risk factors in a logistic regression analysis. Conclusions: Our results demonstrated that Cyr61 was significantly increased in PAD patients with T2DM and that Cyr61 levels were positively associated with disease severity. Cyr61 could be a promising biomarker and further studies are needed to assess its clinical utility. [ABSTRACT FROM AUTHOR]

Purpose – The purpose of this paper is to develop a spectral element modeling to predict electromechanical admittance in the surface-bonded piezoelectric wafer and beam structure considering temperature effects. Design/methodology/approach – For modeling the beam, the axial and transverse vibrations of the beam have been considered, and temperature-dependent mechanical and electromechanical properties of piezoelectric wafer active sensor and aluminum have been analyzed. The influences of temperature effects on electromechanical admittance are investigated. Findings – The results show that a frequency left shift and a decrease in amplitude of admittance in any natural frequencies with increasing temperature have been observed. The mechanism of these changes is discussed. Originality/value – The numerical results may be considered helpful for structural health monitoring using electromechanical impedance technique. [ABSTRACT FROM AUTHOR]

Ultrasonic guided wave non-destructive testing technology, as one of the most widely used techniques in non-destructive testing, has encountered challenges in breaking the diffraction limit to achieve finer super-resolution imaging during its development. As a type of wave accompanying ultrasonic guided wave damage detection, the transient wave has had the potential to achieve super-resolution imaging in recent years due to its high wave number and sub-wavelength characteristics. In order to overcome its spatial attenuation characteristics, this study constructs a periodic plate-column structure metamaterial. By utilizing its resonance with sub-wavelength Bloch modes at specific frequencies and the interaction with transient waves carrying sub-wavelength information, the wave carrying sub-wavelength signals is amplified, transformed, and transmitted to the far field, thereby achieving super-resolution imaging. The dispersion characteristics of the periodic plate-column structure were simulated and experimentally investigated. The super-resolution imaging effect of the periodic plate-column structure metamaterial was simulated using the time-reversal imaging method and experimentally validated, providing a basis for achieving damage super-resolution imaging with periodic plate-column structures. [ABSTRACT FROM AUTHOR]

Background: Heart failure with preserved ejection fraction (HFpEF) is a common disease with high morbidity and lacks effective treatment. We investigated the protective effects of the long-term application of the sodium-glucose cotransporter 2 inhibitor (SGLT2i) dapagliflozin on diabetes-associated HFpEF in a rat model. Serum proteomics and metabolomics analysis were also conducted in type 2 diabetic patients with HFpEF treated with dapagliflozin. Methods: Male Zucker diabetic fatty (ZDF) rats were used as a model of diabetic cardiomyopathy. From weeks 16 to 28, animals were given a vehicle or dapagliflozin (1 mg/kg) once daily. Primary blood biochemistry indices, echocardiography, histopathology, and cardiac hemodynamics were determined during the study period. The key markers of myocardial fibrosis, nitro-oxidative stress, inflammation, apoptosis, autophagy, and AMPK/mTOR signaling were examined. Additionally, healthy controls and individuals with type 2 diabetes were enrolled and 16 serum samples from 4 groups were randomly selected. Serum proteome and metabolome changes after dapagliflozin treatment were analyzed in diabetic individuals with HFpEF. Results: Dapagliflozin effectively prevented the development of HFpEF in rats with diabetes by mitigating nitro-oxidative stress, pro-inflammatory cytokines, myocardial hypertrophy, and fibrosis, reducing apoptosis, and restoring autophagy through AMPK activating and mTOR pathway repressing. Proteomics and metabolomics revealed that cholesterol and high-density lipoprotein particle metabolism, nicotinate and nicotinamide metabolism, arginine biosynthesis, and cAMP and peroxisome proliferator-activated receptor (PPAR) signaling are the major disturbed pathways in HFpEF patients treated with dapagliflozin. Conclusion: Long-term treatment with dapagliflozin significantly prevented the development of HFpEF in diabetic rats. Dapagliflozin could be a promising therapeutic strategy in managing HFpEF individuals with type 2 diabetes. [ABSTRACT FROM AUTHOR]

Thin-walled structures, like aircraft skins and ship shells, are often several meters in size but only a few millimeters thick. By utilizing the laser ultrasonic Lamb wave detection method (LU-LDM), signals can be detected over long distances without physical contact. Additionally, this technology offers excellent flexibility in designing the measurement point distribution. The characteristics of LU-LDM are first analyzed in this review, specifically in terms of laser ultrasound and hardware configuration. Next, the methods are categorized based on three criteria: the quantity of collected wavefield data, the spectral domain, and the distribution of measurement points. The advantages and disadvantages of multiple methods are compared, and the suitable conditions for each method are summarized. Thirdly, we summarize four combined methods that balance detection efficiency and accuracy. Finally, several future development trends are suggested, and the current gaps and shortcomings in LU-LDM are highlighted. This review builds a comprehensive framework for LU-LDM for the first time, which is expected to serve as a technical reference for applying this technology in large, thin-walled structures. [ABSTRACT FROM AUTHOR]

Abstract: AZ91D magnesium alloy samples were treated by micro-arc discharge oxidation (MDO) using two different voltage modes: direct current (dc) and high-frequency bipolar pulsing (BP) to improve the surface properties. The structure, composition, and mechanical characteristics of the oxide films prepared using the same average current density were investigated by scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS), X-ray diffraction (XRD), optical microscopy, profilometry, as well as microhardness and pin-on-disk tests. Both the dc and BP oxide films have mainly the MgO phases and improved microhardness and lower wear rates compared to the untreated Mg alloy. However, the oxidation rate, composition, and structure are different for the two voltage modes. For the same treatment time, the BP mode gives a higher oxidation rate. The BP film is denser and has higher micro-hardness, lower friction coefficient, and smaller weight loss against steel in the scratch test. Our results thus indicate that high-frequency, bipolar pulsing MDO yields a better coating on the Mg alloy. [Copyright &y& Elsevier]

Large plate shell structures are key supporting components for aerospace and transportation equipment. Rapid localization of damages in large-scale thin-walled structures is an urgently needed technology for on-site detection and maintenance. This paper proposed a rapid damage localization method based on a laser ultrasonic circular arrays, combining the advantages of the circular arrays and the MUSIC algorithm. It does not need reference signals in advance, the direct waves and scattered waves can be quickly distinguished and extracted according to the time of arrival (TOA). Then, the two-dimensional multiple signal classification method(2D-MUSIC) under near-field conditions is used to quickly locate damage, which is a feature subspace algorithm in array signal processing technology. Compared with other commonly used imaging algorithms, this method has great advantages in detection efficiency and angular resolution. According to the detection method proposed in this paper, the position of single damage and multiple incoherent damages within 0.12 m2 can be quickly found within 1.3 s, and the angle error and distance error are not more than 5° and 5 cm. However, for the damage of coherent sources, there may be artifacts interfering with the imaging results. [Display omitted] • This paper proposed a rapid damage localization method, combining the circular arrays and the MUSIC algorithm. • The direct waves and scattered waves can be quickly distinguished and extracted according to the time of arrival. • The damages within 0.12 m2 can be quickly found within 1.3 s, the angle and distance error are not more than 5° and 5 cm. [ABSTRACT FROM AUTHOR]

Abstract: Taking into account the effects of thermal diffusion and optical penetration, as well as the finite width and duration of the laser source, the laser-generated ultrasonic force source at surface vicinity is presented. The full acoustic fields of laser-generated ultrasonic bulk wave are obtained and displayed in transversely isotropic plate. The features of laser-generated ultrasound bulk waves are analyzed. The features of laser-generated ultrasonic bulk wave are in good agreement with the theoretical results (the phase velocity surfaces), demonstrating the validity of this simulation. The numerical results indicate that the features of laser-generated ultrasound waveforms in anisotropic specimen, different from the case in isotropic materials, have a close relation with the propagating plane and propagation direction. This method can provide insight to the generation and propagation of laser-generated ultrasonic bulk wave in transversely isotropic material. [Copyright &y& Elsevier]

Background: Heart failure with preserved ejection fraction (HFpEF) is a difficult disease with high morbidity and mortality rates and lacks an effective treatment. Here, we report the therapeutic effect of dapagliflozin, a sodium-glucose cotransporter 2 inhibitor (SGLT2i), on hypertension + hyperlipidemia-induced HFpEF in a pig model. Methods: HFpEF pigs were established by infusing a combination of deoxycorticosterone acetate (DOCA) and angiotensin II (Ang II), and Western diet (WD) feeding for 18 weeks. In the 9th week, half of the HFpEF pigs were randomly assigned to receive additional dapagliflozin treatment (10 mg/day) by oral gavage daily for the next 9 weeks. Blood pressure, lipid levels, echocardiography and cardiac hemodynamics for cardiac structural and functional changes, as well as epinephrine and norepinephrine concentrations in the plasma and tissues were measured. After sacrifice, cardiac fibrosis, the distribution of tyrosine hydroxylase (TH), inflammatory factors (IL-6 and TNF-α) and NO-cGMP-PKG pathway activity in the cardiovascular system were also determined. Results: Blood pressure, total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL) were markedly increased in HFpEF pigs, but only blood pressure was significantly decreased after 9 weeks of dapagliflozin treatment. By echocardiographic and hemodynamic assessment, dapagliflozin significantly attenuated heart concentric remodeling in HFpEF pigs, but failed to improve diastolic function and compliance with the left ventricle (LV). In the dapagliflozin treatment group, TH expression and norepinephrine concentration in the aorta were strongly mitigated compared to that in the HFpEF group. Moreover, inflammatory cytokines such as IL-6 and TNF-α in aortic tissue were markedly elevated in HFpEF pigs and inhibited by dapagliflozin. Furthermore, the reduced expression of eNOS and the PKG-1 protein and the cGMP content in the aortas of HFpEF pigs were significantly restored after 9 weeks of dapagliflozin treatment. Conclusion: 9 weeks of dapagliflozin treatment decreases hypertension and reverses LV concentric remodeling in HFpEF pigs partly by restraining sympathetic tone in the aorta, leading to inhibition of the inflammatory response and NO-cGMP-PKG pathway activation. [ABSTRACT FROM AUTHOR]

Background: FGF21 is a critical endogenous regulator in energy homeostasis and systemic glucose and lipid metabolism. Despite intensive study of the metabolic functions of FGF21, its important role in heart disease needs further exploration. Apoptosis induced by ox-LDL in vascular endothelial cells is an important step in the progress of atherosclerosis. Methods: The effects of FGF21 treatment on apoptosis induced by ox-LDL were tested in HUVECs. The role of FGF21 in atherosclerosis was studied by evaluating its function in apolipoprotein E double knockout (apoE−/−) mice. Results: We found that apoptosis in HUVECs was alleviated by FGF21 treatment. The effects of FGF21 were independent of the ERK1/2 pathway and were mediated through inhibition of the Fas signaling pathway. FGF21 suppressed the development of atherosclerosis, and the administration of FGF21 ameliorated Fas-mediated apoptosis in apoE−/− mice. Conclusion: FGF21 protects against apoptosis in HUVECs by suppressing the expression of Fas; furthermore, FGF21 alleviated atherosclerosis by ameliorating Fas-mediated apoptosis in apoE−/− mice. [ABSTRACT FROM AUTHOR]