18 results on '"Xiuling Lv"'
Search Results
2. Integrated analysis reveals critical glycolytic regulators in hepatocellular carcinoma
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Chenying Lu, Shiji Fang, Qiaoyou Weng, Xiuling Lv, Miaomiao Meng, Jinyu Zhu, Liyun Zheng, Yumin Hu, Yang Gao, Xulu Wu, Jianting Mao, Bufu Tang, Zhongwei Zhao, Li Huang, and Jiansong Ji
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Liver cancer ,Tumor metabolism ,Energy metabolism ,SPP1 ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background Cancer cells primarily utilize aerobic glycolysis for energy production, a phenomenon known as the Warburg effect. Increased aerobic glycolysis supports cancer cell survival and rapid proliferation and predicts a poor prognosis in cancer patients. Methods Molecular profiles from The Cancer Genome Atlas (TCGA) cohort were used to analyze the prognostic value of glycolysis gene signature in human cancers. Gain- and loss-of-function studies were performed to key drivers implicated in hepatocellular carcinoma (HCC) glycolysis. The molecular mechanisms underlying Osteopontin (OPN)-mediated glycolysis were investigated by real-time qPCR, western blotting, immunohistochemistry, luciferase reporter assay, and xenograft and diethyl-nitrosamine (DEN)-induced HCC mouse models. Results Increased glycolysis predicts adverse clinical outcome in many types of human cancers, especially HCC. Then, we identified a handful of differentially expressed genes related to HCC glycolysis. Gain- and loss-of-function studies showed that OPN promotes, while SPP2, LECT2, SLC10A1, CYP3A4, HSD17B13, and IYD inhibit HCC cell glycolysis as revealed by glucose utilization, lactate production, and extracellular acidification ratio. These glycolysis-related genes exhibited significant tumor-promoting or tumor suppressive effect on HCC cells and these effects were glycolysis-dependent. Mechanistically, OPN enhanced HCC glycolysis by activating the αvβ3-NF-κB signaling. Genetic or pharmacological blockade of OPN-αvβ3 axis suppressed HCC glycolysis in xenograft tumor model and hepatocarcinogenesis induced by DEN. Conclusions Our findings reveal crucial determinants for controlling the Warburg metabolism in HCC cells and provide a new insight into the oncogenic roles of OPN in HCC. Video Abstract
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- 2020
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3. Correction to: Integrated analysis reveals critical glycolytic regulators in hepatocellular carcinoma
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Chenying Lu, Shiji Fang, Qiaoyou Weng, Xiuling Lv, Miaomiao Meng, Jinyu Zhu, Liyun Zheng, Yumin Hu, Yang Gao, Xulu Wu, Jianting Mao, Bufu Tang, Zhongwei Zhao, Li Huang, and Jiansong Ji
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Medicine ,Cytology ,QH573-671 - Abstract
An amendment to this paper has been published and can be accessed via the original article.
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- 2020
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4. Identifying Apoptosis-Related Transcriptomic Aberrations and Revealing Clinical Relevance as Diagnostic and Prognostic Biomarker in Hepatocellular Carcinoma
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Jinyu Zhu, Bufu Tang, Xiuling Lv, Miaomiao Meng, Qiaoyou Weng, Nannan Zhang, Jie Li, Kai Fan, Liyun Zheng, Shiji Fang, Min Xu, and Jiansong Ji
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apoptosis ,hepatocellular carcinoma ,overall survival ,relapse-free survival ,diagnosis ,prognosis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
In view of the unsatisfactory treatment outcome of liver cancer under current treatment, where the mortality rate is high and the survival rate is poor, in this study we aimed to use RNA sequencing data to explore potential molecular markers that can be more effective in predicting diagnosis and prognosis of hepatocellular carcinoma. RNA sequencing data and corresponding clinical information were obtained from multiple databases. After matching with the apoptotic genes from the Deathbase database, 14 differentially expressed human apoptosis genes were obtained. Using univariate and multivariate Cox regression analyses, two apoptosis genes (BAK1 and CSE1L) were determined to be closely associated with overall survival (OS) in HCC patients. And subsequently experiments also validated that knockdown of BAK1 and CSE1L significantly inhibited cell proliferation and promoted apoptosis in the HCC. Then the two genes were used to construct a prognostic signature and diagnostic models. The high-risk group showed lower OS time compared to low-risk group in the TCGA cohort (P < 0.001, HR = 2.11), GSE14520 cohort (P = 0.003, HR = 1.85), and ICGC cohort (P < 0.001, HR = 4). And the advanced HCC patients showed higher risk score and worse prognosis compared to early-stage HCC patients. Moreover, the prognostic signature was validated to be an independent prognostic factor. The diagnostic models accurately predicted HCC from normal tissues and dysplastic nodules in the training and validation cohort. These results indicated that the two apoptosis-related signature effectively predicted diagnosis and prognosis of HCC and may serve as a potential biomarker and therapeutic target for HCC.
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- 2021
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5. Piperlongumine, a Novel TrxR1 Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cells by ROS-Mediated ER Stress
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Qianqian Zhang, Weiqian Chen, Xiuling Lv, Qiaoyou Weng, Minjiang Chen, Ri Cui, Guang Liang, and Jiansong Ji
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thioredoxin reductase 1 ,reactive oxygen species ,hepatocellular carcinoma ,endoplasmic reticulum stress ,piperlongumine ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third leading cause of cancer-related deaths globally. Despite advances in diagnosis and treatment, the incidence and mortality of HCC continue to rise. Piperlongumine (PL), an alkaloid isolated from the fruit of the long pepper, is known to selectively kill tumor tissues while sparing their normal counterparts. However, the killing effects of PL on HCC and the underlying mechanism of PL are not clear. We report that PL may interact with thioredoxin reductase 1 (TrxR1), an important selenocysteine (Sec)-containing antioxidant enzyme, and induce reactive oxygen species (ROS)-mediated apoptosis in HCC cells. Our results suggest that PL induces a lethal endoplasmic reticulum (ER) stress response in HCC cells by targeting TrxR1 and increasing intracellular ROS levels. Notably, PL treatment reduces TrxR1 activity and tumor cell burden in vivo. Additionally, TrxR1 is significantly upregulated in existing HCC databases and available HCC clinical specimens. Taken together, these results suggest PL as a novel anticancer candidate for the treatment of HCC. More importantly, this study reveals that TrxR1 might be an effective target in treating HCC.
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- 2019
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6. Integrated analysis reveals critical glycolytic regulators in hepatocellular carcinoma
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Bufu Tang, Jinyu Zhu, Yang Gao, Shiji Fang, Jianting Mao, Liyun Zheng, Zhongwei Zhao, Qiaoyou Weng, Jiansong Ji, Xiuling Lv, Li Huang, Xulu Wu, Miaomiao Meng, Chenying Lu, and Yumin Hu
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Cell ,lcsh:Medicine ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Glycolysis ,Osteopontin ,lcsh:QH573-671 ,Molecular Biology ,Tumor metabolism ,030304 developmental biology ,0303 health sciences ,lcsh:Cytology ,Research ,lcsh:R ,Cell Biology ,Energy metabolism ,Gene signature ,medicine.disease ,Warburg effect ,digestive system diseases ,medicine.anatomical_structure ,Anaerobic glycolysis ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,biology.protein ,Liver cancer ,SPP1 - Abstract
Background Cancer cells primarily utilize aerobic glycolysis for energy production, a phenomenon known as the Warburg effect. Increased aerobic glycolysis supports cancer cell survival and rapid proliferation and predicts a poor prognosis in cancer patients. Methods Molecular profiles from The Cancer Genome Atlas (TCGA) cohort were used to analyze the prognostic value of glycolysis gene signature in human cancers. Gain- and loss-of-function studies were performed to key drivers implicated in hepatocellular carcinoma (HCC) glycolysis. The molecular mechanisms underlying Osteopontin (OPN)-mediated glycolysis were investigated by real-time qPCR, western blotting, immunohistochemistry, luciferase reporter assay, and xenograft and diethyl-nitrosamine (DEN)-induced HCC mouse models. Results Increased glycolysis predicts adverse clinical outcome in many types of human cancers, especially HCC. Then, we identified a handful of differentially expressed genes related to HCC glycolysis. Gain- and loss-of-function studies showed that OPN promotes, while SPP2, LECT2, SLC10A1, CYP3A4, HSD17B13, and IYD inhibit HCC cell glycolysis as revealed by glucose utilization, lactate production, and extracellular acidification ratio. These glycolysis-related genes exhibited significant tumor-promoting or tumor suppressive effect on HCC cells and these effects were glycolysis-dependent. Mechanistically, OPN enhanced HCC glycolysis by activating the αvβ3-NF-κB signaling. Genetic or pharmacological blockade of OPN-αvβ3 axis suppressed HCC glycolysis in xenograft tumor model and hepatocarcinogenesis induced by DEN. Conclusions Our findings reveal crucial determinants for controlling the Warburg metabolism in HCC cells and provide a new insight into the oncogenic roles of OPN in HCC.
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- 2020
7. Identification and validation of the angiogenic genes for constructing diagnostic, prognostic, and recurrence models for hepatocellular carcinoma
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Jinyu Zhu, Yueli Shi, Min Xu, Miaomiao Meng, Jie Li, Minjiang Chen, Nannan Zhang, Kai Fan, Xiuling Lv, Bufu Tang, Qiaoyou Weng, and Jiansong Ji
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Oncology ,Male ,Aging ,medicine.medical_specialty ,MMP3 ,recurrence ,Carcinoma, Hepatocellular ,Angiogenesis ,diagnosis ,chemistry.chemical_compound ,angiogenesis ,hepatocellular carcinoma (HCC) ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,RNA, Neoplasm ,Gene ,Piperlongumine ,business.industry ,Gene Expression Profiling ,Liver Neoplasms ,Cell Biology ,Nomogram ,medicine.disease ,Prognosis ,Training cohort ,Gene Expression Regulation, Neoplastic ,chemistry ,Hepatocellular carcinoma ,Female ,Neoplasm Recurrence, Local ,business ,Transcriptome ,Selection operator ,Research Paper - Abstract
Since angiogenesis has an indispensable effect in the development and progression of tumors, in this study we aimed to identify angiogenic genes closely associated with prognosis of HCC to establish diagnostic, prognostic, and recurrence models. We analyzed 132 angiogenic genes and HCC-related RNA sequence data from the TCGA and ICGC databases by Cox and least absolute shrinkage and selection operator (LASSO) regression, and identified four angiogenic genes (ENFA3, EGF, MMP3 and AURKB) to establish prognosis, recurrence and diagnostic models and corresponding nomograms. The prognostic and recurrence models were determined to be independent predictors of prognosis and recurrence (P < 0.05). And compared with the low-risk group, patients in the high-risk group had worse overall survival (OS) rates in training cohort (P < 0.001) and validation cohort (P < 0.001), and higher recurrence rates in training cohort (P
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- 2020
8. HIF-2α-targeted interventional chemoembolization multifunctional microspheres for effective elimination of hepatocellular carcinoma
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Minjiang Chen, Gaofeng Shu, Xiuling Lv, Xiaoling Xu, Chenying Lu, Enqi Qiao, Shiji Fang, Lin Shen, Nannan Zhang, Jun Wang, Chunmiao Chen, Jingjing Song, Zhuang Liu, Yongzhong Du, and Jiansong Ji
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Vascular Endothelial Growth Factor A ,Carcinoma, Hepatocellular ,Liver Neoplasms ,Biophysics ,Bioengineering ,Microspheres ,Biomaterials ,Mechanics of Materials ,Doxorubicin ,Ceramics and Composites ,Basic Helix-Loop-Helix Transcription Factors ,Tumor Microenvironment ,Humans ,Chemoembolization, Therapeutic ,Hypoxia - Abstract
Transcatheter arterial chemoembolization (TACE) is widely used for the treatment of advanced hepatocellular carcinoma (HCC). However, the long-term hypoxic microenvironment caused by TACE seriously affects the therapeutic effect of TACE. HIF-2α plays a crucial role on the chronic hypoxia process, which might be an ideal target for TACE therapy. Herein, a multifunctional polyvinyl alcohol (PVA)/hyaluronic acid (HA)-based microsphere (PT/DOX-MS) co-loaded with doxorubicin (DOX) and PT-2385, an effective HIF-2α inhibitor, was developed for enhanced TACE treatment efficacy. In vitro and in vivo studies revealed that PT/DOX-MS had a superior ability to treat HCC by blocking the tumor cells in G2/M phase, prompting cell apoptosis, and inhibiting tumor angiogenesis. The antitumor mechanisms of PT/DOX-MS were possibly due to that the introduction of PT-2385 could effectively inhibit the expression level of HIF-2α in hypoxic HCC cells, thereby down-regulating the expression levels of Cyclin D1, VEGF and TGF-α. In addition, the combination of DOX and PT-2385 could jointly inhibit VEGF expression, which was another reason accounting for the combined anti-cancer effect of PT/DOX-MS. Overall, our study demonstrated that PT/DOX-MS is a promising embolic agent for enhanced HCC treatment via the combined effect of hypoxia microenvironment improvement, chemotherapy, and embolization.
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- 2021
9. Construction of a novel radiomics nomogram for the prediction of aggressive intrasegmental recurrence of HCC after radiofrequency ablation
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Minjiang Chen, Miaomiao Meng, Fazong Wu, Weichuan Ye, Chunli Kong, Gaofeng Shu, Chunmiao Chen, Liyun Zheng, Zhongwei Zhao, Jianfei Tu, Qiaoyou Weng, Xiuling Lv, Jiansong Ji, Shiji Fang, and Shimiao Cheng
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medicine.medical_specialty ,Radiofrequency Ablation ,Carcinoma, Hepatocellular ,medicine.diagnostic_test ,Radiofrequency ablation ,business.industry ,Liver Neoplasms ,Univariate ,Magnetic resonance imaging ,General Medicine ,Nomogram ,medicine.disease ,Logistic regression ,law.invention ,Nomograms ,Radiomics ,Decision curve analysis ,law ,Hepatocellular carcinoma ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Radiology ,business ,Retrospective Studies - Abstract
OBJECTIVES To construct a precise prediction model of preoperative magnetic resonance imaging (MRI)-based nomogram for aggressive intrasegmental recurrence (AIR) of hepatocellular carcinoma (HCC) patients treated with radiofrequency ablation (RFA). METHODS Among 891 patients with HCC treated by RFA, 22 patients with AIR and 36 patients without AIR (non-AIR) were finally enrolled in our study, and each patient was followed up for more than 6 months to determine the occurrence of AIR. The laboratory indicators and MRI features were compared and assessed. Preoperative contrast-enhanced T1-weighted images (CE-T1WI) were used for radiomics analysis. The selected clinical indicators and texture features were finally screened out to generate the novel prediction nomogram. RESULTS Tumor shape, ADC Value, DWI signal intensity and ΔSI were selected as the independent factors of AIR by univariate and multivariate logistic regression analysis. Meanwhile, two radiomics features were selected from 396 candidate features by LASSO (P
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- 2021
10. Correction to: Integrated analysis reveals critical glycolytic regulators in hepatocellular carcinoma
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Yang Gao, Jianting Mao, Qiaoyou Weng, Bufu Tang, Miaomiao Meng, Jiansong Ji, Shiji Fang, Xiuling Lv, Chenying Lu, Liyun Zheng, Jinyu Zhu, Zhongwei Zhao, Li Huang, Xulu Wu, and Yumin Hu
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Male ,Carcinoma, Hepatocellular ,lcsh:Medicine ,Mice, Nude ,Biology ,Biochemistry ,Cell Line, Tumor ,medicine ,Warburg Effect, Oncologic ,Animals ,Humans ,Glycolysis ,lcsh:QH573-671 ,Receptor ,Molecular Biology ,Cell Proliferation ,Mice, Inbred BALB C ,lcsh:Cytology ,Gene Expression Profiling ,lcsh:R ,Liver Neoplasms ,NF-kappa B ,Correction ,Cell Biology ,medicine.disease ,Integrin alphaVbeta3 ,Prognosis ,Gene Expression Regulation, Neoplastic ,Hepatocellular carcinoma ,Cancer research ,Osteopontin ,Signal Transduction - Abstract
Cancer cells primarily utilize aerobic glycolysis for energy production, a phenomenon known as the Warburg effect. Increased aerobic glycolysis supports cancer cell survival and rapid proliferation and predicts a poor prognosis in cancer patients.Molecular profiles from The Cancer Genome Atlas (TCGA) cohort were used to analyze the prognostic value of glycolysis gene signature in human cancers. Gain- and loss-of-function studies were performed to key drivers implicated in hepatocellular carcinoma (HCC) glycolysis. The molecular mechanisms underlying Osteopontin (OPN)-mediated glycolysis were investigated by real-time qPCR, western blotting, immunohistochemistry, luciferase reporter assay, and xenograft and diethyl-nitrosamine (DEN)-induced HCC mouse models.Increased glycolysis predicts adverse clinical outcome in many types of human cancers, especially HCC. Then, we identified a handful of differentially expressed genes related to HCC glycolysis. Gain- and loss-of-function studies showed that OPN promotes, while SPP2, LECT2, SLC10A1, CYP3A4, HSD17B13, and IYD inhibit HCC cell glycolysis as revealed by glucose utilization, lactate production, and extracellular acidification ratio. These glycolysis-related genes exhibited significant tumor-promoting or tumor suppressive effect on HCC cells and these effects were glycolysis-dependent. Mechanistically, OPN enhanced HCC glycolysis by activating the αvβ3-NF-κB signaling. Genetic or pharmacological blockade of OPN-αvβ3 axis suppressed HCC glycolysis in xenograft tumor model and hepatocarcinogenesis induced by DEN.Our findings reveal crucial determinants for controlling the Warburg metabolism in HCC cells and provide a new insight into the oncogenic roles of OPN in HCC. Video Abstract.
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- 2020
11. Prediction of tumor response via a pretreatment MRI radiomics-based nomogram in HCC treated with TACE
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Chunli Kong, Xihui Ying, Jingjing Song, Shiji Fang, Qiaoyou Weng, Jianfei Tu, Wei Weng, Gaofeng Shu, Miaoqing Ye, Jiansong Ji, Xiuling Lv, Weiyue Chen, Minjiang Chen, and Zhongwei Zhao
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medicine.medical_specialty ,Carcinoma, Hepatocellular ,Hepatocellular carcinoma ,Feature selection ,Nomogram ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Lasso (statistics) ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Chemoembolization, Therapeutic ,Retrospective Studies ,Receiver operating characteristic ,Interventional ,business.industry ,Therapeutic chemoembolization ,Liver Neoplasms ,General Medicine ,medicine.disease ,Prognosis ,Magnetic Resonance Imaging ,BCLC Stage ,Regression ,Nomograms ,Feature (computer vision) ,030220 oncology & carcinogenesis ,Radiology ,business - Abstract
Objectives To develop and validate a pre-transcatheter arterial chemoembolization (TACE) MRI-based radiomics model for predicting tumor response in intermediate-advanced hepatocellular carcinoma (HCC) patients. Materials Ninety-nine intermediate-advanced HCC patients (69 for training, 30 for validation) treated with TACE were enrolled. MRI examinations were performed before TACE, and the efficacy was evaluated according to the mRECIST criterion 3 months after TACE. A total of 396 radiomics features were extracted from T2-weighted pre-TACE images, and least absolute shrinkage and selection operator (LASSO) regression was applied to feature selection and model construction. The performance of the model was evaluated by receiver operating characteristic (ROC) curves, calibration curves, and decision curves. Results The AFP value, Child-Pugh score, and BCLC stage showed a significant difference between the TACE response (TR) and non-TACE response (nTR) patients. Six radiomics features were selected by LASSO and the radiomics score (Rad-score) was calculated as the sum of each feature multiplied by the non-zero coefficient from LASSO. The AUCs of the ROC curve based on Rad-score were 0.812 and 0.866 in the training and validation cohorts, respectively. To improve the diagnostic efficiency, the Rad-score was further integrated with the above clinical indicators to form a novel predictive nomogram. Results suggested that the AUC increased to 0.861 and 0.884 in the training and validation cohorts, respectively. Decision curve analysis showed that the radiomics nomogram was clinically useful. Conclusion The radiomics and clinical indicator-based predictive nomogram can well predict TR in intermediate-advanced HCC and can further be applied for auxiliary diagnosis of clinical prognosis. Key Points • The therapeutic outcome of TACE varies greatly even for patients with the same clinicopathologic features. • Radiomics showed excellent performance in predicting the TACE response. • Decision curves demonstrated that the novel predictive model based on the radiomics signature and clinical indicators has great clinical utility.
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- 2020
12. Gadolinium-loaded calcium phosphate nanoparticles for magnetic resonance imaging of orthotopic hepatocarcinoma and primary hepatocellular carcinoma
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Jie Li, Nannan Zhang, Yong-Zhong Du, Chunmiao Chen, Qiaoyou Weng, Minjiang Chen, Xiao-Ling Xu, Xiuling Lv, Wei Weng, Chenying Lu, Bufu Tang, Gaofeng Shu, and Jiansong Ji
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Calcium Phosphates ,Male ,Carcinoma, Hepatocellular ,Biocompatibility ,Gadolinium ,MRI contrast agent ,Biomedical Engineering ,chemistry.chemical_element ,Contrast Media ,02 engineering and technology ,Cell-Penetrating Peptides ,Calcium ,03 medical and health sciences ,Mice ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,General Materials Science ,Particle Size ,Early Detection of Cancer ,030304 developmental biology ,0303 health sciences ,medicine.diagnostic_test ,Liver Neoplasms ,Magnetic resonance imaging ,Hep G2 Cells ,021001 nanoscience & nanotechnology ,medicine.disease ,Magnetic Resonance Imaging ,chemistry ,Hepatocellular carcinoma ,Cancer research ,Nanoparticles ,Female ,0210 nano-technology ,Liver cancer ,Neoplasm Transplantation - Abstract
The development of magnetic resonance imaging (MRI) contrast agents with high sensitivity and good biocompatibility is of great value for the diagnosis of primary hepatocellular carcinoma (HCC). Here, a novel MRI contrast agent based on calcium phosphate (CaP) nanoparticles modified with a liver cancer cell targeting peptide A54 (A54-CaP) was fabricated. The T1-positive contrast agent Gd-DTPA was encapsulated inside the nanoparticles (A54-CaPNPs), with a mean diameter of 30 nm and a high encapsulation efficiency of 92.73%. The A54-CaPNP solution exhibited higher longitudinal relaxivity (6.07 mM-1 s-1) than that of the clinically used MRI contrast agent Gd-DTPA (3.56 mM-1 s-1). A much higher accumulation of the nanoparticles in the liver cells was observed, which was directed by the A54 targeting peptide. Furthermore, the MRI diagnostic efficiency of A54-CaPNPs was systematically investigated in an orthotopic liver cancer model and primary HCC model. In vivo MRI experiments showed that A54-CaPNPs had higher sensitivity in the BEL-7402 orthotopic liver cancer model with a more remarkable contrast enhancement and a longer imaging time compared to those without A54 modification. Moreover, the experiments on primary HCC models suggested that A54-CaPNPs showed greatly enhanced MR imaging performance in comparison with Gd-DTPA. These results suggest that A54-CaPNPs possess great potential to enable the non-invasive early diagnosis of primary HCC for timely surgical resection.
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- 2020
13. Additional file 2 of Integrated analysis reveals critical glycolytic regulators in hepatocellular carcinoma
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Chenying Lu, Shiji Fang, Qiaoyou Weng, Xiuling Lv, Miaomiao Meng, Zhu, Jinyu, Liyun Zheng, Yumin Hu, Gao, Yang, Xulu Wu, Jianting Mao, Bufu Tang, Zhongwei Zhao, Huang, Li, and Jiansong Ji
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digestive system diseases - Abstract
Additional file 1 Supplementary Fig. 1 The prognostic value of glycolysis-related genes in HCC. Supplementary Fig. 2 The correlation between glycolysis-related genes and the glycolysis signature. Supplementary Fig. 3 Expression pattern and roles of OPN in HCC cells. Supplementary Fig. 4 Expression pattern and prognostic value of OPN in HCC tissues. Supplementary Table 1: Primers used in this study. Supplementary Table 2: Group information.
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- 2020
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14. Piperlongumine, a Novel TrxR1 Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cells by ROS-Mediated ER Stress
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Jiansong Ji, Ri Cui, Qianqian Zhang, Xiuling Lv, Guang Liang, Qiaoyou Weng, Minjiang Chen, and Weiqian Chen
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0301 basic medicine ,piperlongumine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,thioredoxin reductase 1 ,Pharmacology (medical) ,neoplasms ,Piperlongumine ,Original Research ,chemistry.chemical_classification ,Pharmacology ,reactive oxygen species ,Reactive oxygen species ,Endoplasmic reticulum ,lcsh:RM1-950 ,Cancer ,hepatocellular carcinoma ,medicine.disease ,digestive system diseases ,030104 developmental biology ,lcsh:Therapeutics. Pharmacology ,chemistry ,Apoptosis ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer research ,Unfolded protein response ,endoplasmic reticulum stress - Abstract
Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third leading cause of cancer-related deaths globally. Despite advances in diagnosis and treatment, the incidence and mortality of HCC continue to rise. Piperlongumine (PL), an alkaloid isolated from the fruit of the long pepper, is known to selectively kill tumor tissues while sparing their normal counterparts. However, the killing effects of PL on HCC and the underlying mechanism of PL are not clear. We report that PL may interact with thioredoxin reductase 1 (TrxR1), an important selenocysteine (Sec)-containing antioxidant enzyme, and induce reactive oxygen species (ROS)-mediated apoptosis in HCC cells. Our results suggest that PL induces a lethal endoplasmic reticulum (ER) stress response in HCC cells by targeting TrxR1 and increasing intracellular ROS levels. Notably, PL treatment reduces TrxR1 activity and tumor cell burden in vivo. Additionally, TrxR1 is significantly upregulated in existing HCC databases and available HCC clinical specimens. Taken together, these results suggest PL as a novel anticancer candidate for the treatment of HCC. More importantly, this study reveals that TrxR1 might be an effective target in treating HCC.
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- 2019
15. Piperlongumine Achieves Antitumor Effects by Targeting Angiogenic Genes that Have Predictive Value for the Diagnosis, Prognosis and Recurrence of Hepatocellular Carcinoma
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Jie Li, Kai Fan, Miaomiao Meng, Jinyu Zhu, Yueli Shi, Min Xu, Nannan Zhang, Minjiang Chen, Jiansong Ji, Xiuling Lv, Bufu Tang, and Qiaoyou Weng
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Oncology ,medicine.medical_specialty ,MMP3 ,business.industry ,Proportional hazards model ,Angiogenesis ,Cell ,Nomogram ,medicine.disease ,Metastasis ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Hepatocellular carcinoma ,Internal medicine ,medicine ,business ,Piperlongumine - Abstract
Background: In the context of the high recurrence rate and poor clinical prognosis of hepatocellular carcinoma (HCC), and considering the indispensable effect of angiogenesis in the development and metastasis of tumors, we analyzed HCC-related RNA sequence data to identify angiogenic genes closely associated with the prognosis of HCC. We used the identified genes to establish diagnostic, prognostic, and recurrence models to help clinicians effectively develop individualized treatment strategies for HCC. Method: We comprehensively analyzed 132 angiogenic genes and HCC-related RNA sequence data from the TCGA and the ICGC databases by Cox regression and least absolute shrinkage and selection operator(LASSO) regression to identify angiogenic genes; the genes were used to establish prognosis, recurrence and diagnostic models and to construct corresponding predictive nomograms. Pharmacological analysis was performed to detect target angiogenic drugs. Subsequently, in vitro cell experiments and western blot experiments were used to explore the anti-tumor effect and possible molecular mechanism of an angiogenesis-targeting target drug. Results: Four angiogenic genes (ENFA3, EGF, MMP3 and AURKB) were used to establish prognostic, relapse and diagnostic models. Through the prognostic model, we found that the overall survival (OS) of the high-risk group in the training cohort (P < 0.001, HR = 0.33) and validation cohort (P < 0.001, HR = 0.33) were significantly worse than those of the low-risk group. The recurrence model also demonstrated that in the training cohort (P
- Published
- 2019
16. Non-invasive evaluation for benign and malignant subcentimeter pulmonary ground-glass nodules (≤1 cm) based on CT texture analysis
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Hu Xianghua, Jiansong Ji, Zhongxue Li, Minjiang Chen, Jie Li, Qiaoyou Weng, Chunmiao Chen, Xiuling Lv, Peipei Pang, Wei Weng, Min Xu, Shimiao Cheng, and Weichuan Ye
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Male ,medicine.medical_specialty ,Lung Neoplasms ,Adenocarcinoma ,Sensitivity and Specificity ,Texture (geology) ,030218 nuclear medicine & medical imaging ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Diagnostic model ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Retrospective Studies ,Full Paper ,business.industry ,Non invasive ,General Medicine ,Middle Aged ,030220 oncology & carcinogenesis ,Multiple Pulmonary Nodules ,Female ,Radiology ,Tomography ,Tomography, X-Ray Computed ,business - Abstract
Objectives: To investigate potential diagnostic model for predicting benign or malignant status of subcentimeter pulmonary ground-glass nodules (SPGGNs) (≤1 cm) based on CT texture analysis. Methods: A total of 89 SPGGNs from 89 patients were included; 51 patients were diagnosed with adenocarcinoma, and 38 were diagnosed with inflamed or infected benign SPGGNs. Analysis Kit software was used to manually delineate the volume of interest of lesions and extract a total of 396 quantitative texture parameters. The statistical analysis was performed using R software. The SPGGNs were randomly divided into a training set (n = 59) and a validation set (n = 30). All pre-normalized (Z-score) feature values were subjected to dimension reduction using the LASSO algorithm,and the most useful features in the training set were selected. The selected imaging features were then combined into a Rad-score, which was further assessed by ROC curve analysis in the training and validation sets. Results: Four characteristic parameters (ClusterShade_AllDirection_offset4_SD, ShortRunEmphasis_angle45_offset1, Maximum3DDiameter, SurfaceVolumeRatio) were further selected by LASSO (p < 0.05). As a cluster of imaging biomarkers, the above four parameters were used to form the Rad-score. The AUC for differentiating between benign and malignant SPGGNs in the training set was 0.792 (95% CI: 0.671, 0.913), and the sensitivity and specificity were 86.10 and 65.20%, respectively. The AUC in the validation set was 72.9% (95% CI: 0.545, 0.913), and the sensitivity and specificity were 86.70 and 60%, respectively. Conclusion: The present diagnostic model based on the cluster of imaging biomarkers can preferably distinguish benign and malignant SPGGNs (≤1 cm). Advances in knowledge: Texture analysis based on CT images provide a new and credible technique for accurate identification of subcentimeter pulmonary ground-glass nodules.
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- 2020
17. CPSF7 regulates liver cancer growth and metastasis by facilitating WWP2-FL and targeting the WWP2/PTEN/AKT signaling pathway
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Dengke Zhang, Liyun Zheng, Chenchen Mao, Xiuling Lv, Xiaoxi Fan, Jianting Mao, Minjiang Chen, Ye Yani, Wei Weng, Min Xu, Shiji Fang, and Jiansong Ji
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Male ,0301 basic medicine ,Ubiquitin-Protein Ligases ,Transplantation, Heterologous ,Mice, Nude ,Metastasis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Protein Isoforms ,PTEN ,RNA, Small Interfering ,Molecular Biology ,Protein kinase B ,Cell Proliferation ,mRNA Cleavage and Polyadenylation Factors ,Gene knockdown ,biology ,Cell growth ,Akt/PKB signaling pathway ,Liver Neoplasms ,PTEN Phosphohydrolase ,Cell Biology ,Middle Aged ,medicine.disease ,Cell biology ,RNA Recognition Motif Proteins ,030104 developmental biology ,030220 oncology & carcinogenesis ,Hepatic stellate cell ,biology.protein ,Female ,RNA Interference ,Liver cancer ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Alternative splicing within a gene can create different versions of an mRNA, called isoforms. CFIm, composed of a small subunit CFIm25 and two large subunits CFIm68 and CFIm59 (also known as CPSF7), has been proposed as an enhancer-dependent activator of mRNA 3' processing. In this study, we investigated the role of CPSF7 in hepatocellular carcinoma. Experimental evidence suggests that the expression level of CPSF7 is higher in liver cancer cells and tissues than in non-tumor hepatic cells and tissues. Furthermore, knockdown of CPSF7 effectively suppressed cell proliferation, migration and colony formation in liver cancer cells by inhibiting PTEN/AKT signaling. CPSF7 promoted WWP2-FL due to the presence of PTEN ubiquitination sites in this longer transcript. Taken together, we identified that CPSF7 regulates liver cancer growth by targeting WWP2-FL that in turn regulates AKT activation in a PTEN-dependent manner.
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- 2020
18. Research on Innovation Ability Cultivation and Evaluation Index System of Local High Level Technical Personnel
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Xiuling Lv, Fengli Ge, Shanpo Nian, Gong Yuhan, Lei Chen, and Ruikun Gong
- Subjects
Engineering ,Performance appraisal ,Knowledge management ,Index (economics) ,Index system ,business.industry ,media_common.quotation_subject ,Analytic hierarchy process ,Variety (cybernetics) ,Weighting ,Engineering management ,Originality ,Observation point ,business ,media_common - Abstract
How to evaluate innovation ability objectively and scientifically of high level innovative technical personnel is of great significance for an area to draw up scientific and technical policy and originality innovation strategy. By literature review method, analytic hierarchy process and expert scoring method, throuh the basis on analyzing a variety of factors which impact on innovation ability of local high level technical personnel, after two rounds of expert consultation, three level evaluation index system has been established, which includes sixteen two-level indexes and thirty five three-level indexes. The corresponding weighting coefficients and the main observation point are provided by every index. For example postgraduates graduating in 2013 from an university in Hebei province, output and effect evaluation of index system are investigated from performance appraisal, which proved that this index system is scientific and feasible.
- Published
- 2014
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