163 results on '"Xiuhua Wu"'
Search Results
2. A distinct immune landscape in anti-synthetase syndrome profiled by a single-cell genomic study
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Jiayu Ding, Yanmei Li, Zhiqin Wang, Feng Han, Ming Chen, Jun Du, Tong Yang, Mei Zhang, Yingai Wang, Jing Xu, Gaoya Wang, Yong Xu, Xiuhua Wu, Jian Hao, Xinlei Liu, Guangxin Zhang, Na Zhang, Wenwen Sun, Zhigang Cai, and Wei Wei
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anti-synthetase syndrome (ASS) ,single-cell RNA sequencing (scRNA-seq) ,mucosal-associated invariant T (MAIT) cell ,IFN-II ,auto-immune diseases ,Immunologic diseases. Allergy ,RC581-607 - Abstract
ObjectivesThe objective of this study was to profile the transcriptional profiles of peripheral blood mononuclear cells (PBMCs) and their immune repertoires affected by anti-synthetase syndrome (ASS) at the single-cell level.MethodsWe performed single-cell RNA sequencing (scRNA-seq) analysis of PBMCs and bulk RNA sequencing for patients with ASS (N=3) and patients with anti-melanoma differentiation-associated gene 5-positive dermatomyositis (MDA5+ DM, N=3) along with healthy controls (HCs, N=4). As ASS and MDA5+ DM have similar organ involvements, MDA5+ DM was used as a disease control. The immune repertoire was constructed by reusing the same scRNA-seq datasets. Importantly, flow cytometry was performed to verify the results from the scRNA-seq analysis.ResultsAfter meticulous annotation of PBMCs, we noticed a significant decrease in the proportion of mucosal-associated invariant T (MAIT) cells in ASS patients compared to HCs, while there was a notable increase in the proportion of proliferative NKT cells. Compared with MDA5+ DM patients, in their PBMCs ASS patients presented substantial enrichment of interferon pathways, which were primarily mediated by IFN-II, and displayed a weak immune response. Furthermore, ASS patients exhibited more pronounced metabolic abnormalities, which may in turn affect oxidative phosphorylation pathways. Monocytes from ASS patients appear to play a crucial role as receptive signaling cells for the TNF pathway. Immunophenotyping analysis of PBMCs from ASS patients revealed an increasing trend for the clone type CQQSYSTPWTF.ConclusionUsing single-cell genomic datasets of ASS PBMCs, we revealed a distinctive profile in the immune system of individuals with ASS, compared to that with MDA5+ DM or healthy controls.
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- 2024
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3. Discovery of Taurocholic Acid Sodium Hydrate as a Novel Repurposing Drug for Intervertebral Disc Degeneration by Targeting MAPK3
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Ping Li, Zesen Chen, Keyu Meng, Yanlin Chen, Jiajia Xu, Xin Xiang, Xiuhua Wu, Zhiping Huang, Ruijun Lai, Peng Li, Zhongming Lai, Xiang Ao, Zhongyuan Liu, Kaifan Yang, Xiaochun Bai, and Zhongmin Zhang
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Chondrogenesis ,Intervertebral Disc Degeneration (IDD) ,Mesenchymal Stem Cells (MSCs) ,Osteogenesis ,Taurocholic Acid Sodium Salt Hydrate (NAT) ,Orthopedic surgery ,RD701-811 - Abstract
Objective Nowadays, more than 90% of people over 50 years suffer from intervertebral disc degeneration (IDD), but there are exist no ideal drugs. The aim of this study is to identify a new drug for IDD. Methods An approved small molecular drug library including 2040 small molecular compounds was used here. We found that taurocholic acid sodium hydrate (NAT) could induce chondrogenesis and osteogenesis in mesenchymal stem cells (MSCs). Then, an in vivo mouse model of IDD was established and the coccygeal discs transcriptome analysis and surface plasmon resonance analysis (SPR) integrated with liquid chromatography–tandem mass spectrometry assay (LC‐MS) were performed in this study to study the therapy effect and target proteins of NAT for IDD. Micro‐CT was used to evaluate the cancellous bone. The expression of osteogenic (OCN, RNX2), chondrogenic (COL2A1, SOX9), and the target related (ERK1/2, p‐ERK1/2) proteins were detected. The alkaline phosphatase staining was performed to estimate osteogenic differentiation. Blood routine and blood biochemistry indexes were analyzed for the safety of NAT. Results The results showed that NAT could induce chondrogenesis and osteogenesis in MSCs. Further experiments confirmed NAT could ameliorate the secondary osteoporosis and delay the development of IDD in mice. Transcriptome analysis identified 128 common genes and eight Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for NAT. SPR‐LC–MS assay detected 57 target proteins for NAT, including MAPK3 (mitogen‐activated protein kinase 3), also known as ERK1 (extracellular regulated protein kinase 1). Further verification experiment confirmed that NAT significantly reduced the expression of ERK1/2 phosphorylation. Conclusion NAT would induce chondrogenesis and osteogenesis of MSCs, ameliorate the secondary osteoporosis and delay the progression of IDD in mice by targeting MAPK3.Furthermore, MAPK3, especially the phosphorylation of MAPK3, would be a potential therapeutic target for IDD treatment.
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- 2024
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4. METTL3-mediated m6A RNA methylation induces the differentiation of lung resident mesenchymal stem cells into myofibroblasts via the miR-21/PTEN pathway
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Yi Lu, Zeyu Liu, Yunjiao Zhang, Xiuhua Wu, Wei Bian, Shan Shan, Danrong Yang, and Tao Ren
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Lung resident mesenchymal stem cells (LR-MSCs) ,M6A methylation ,Myofibroblast ,METTL3/miR-21/PTEN pathway ,Pulmonary fibrosis (PF) ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background The accumulation of myofibroblasts is the key pathological feature of pulmonary fibrosis (PF). Aberrant differentiation of lung-resident mesenchymal stem cells (LR-MSCs) has been identified as a critical source of myofibroblasts, but the molecular mechanisms underlying this process remain largely unknown. In recent years, N6-methyladenosine (m6A) RNA modification has been implicated in fibrosis development across diverse organs; however, its specific role in promoting the differentiation of LR-MSCs into myofibroblasts in PF is not well defined. Methods In this study, we examined the levels of m6A RNA methylation and the expression of its regulatory enzymes in both TGF-β1-treated LR-MSCs and fibrotic mouse lung tissues. The downstream target genes of m6A and their related pathways were identified according to a literature review, bioinformatic analysis and experimental verification. We also assessed the expression levels of myofibroblast markers in treated LR-MSCs and confirmed the involvement of the above-described pathway in the aberrant differentiation direction of LR-MSCs under TGF-β1 stimulation by overexpressing or knocking down key genes within the pathway. Results Our results revealed that METTL3-mediated m6A RNA methylation was significantly upregulated in both TGF-β1-treated LR-MSCs and fibrotic mouse lung tissues. This process directly led to the aberrant differentiation of LR-MSCs into myofibroblasts by targeting the miR-21/PTEN pathway. Moreover, inhibition of METTL3 or miR-21 and overexpression of PTEN could rescue this abnormal differentiation. Conclusion Our study demonstrated that m6A RNA methylation induced aberrant LR-MSC differentiation into myofibroblasts via the METTL3/miR-21/PTEN signaling pathway. We indicated a novel mechanism to promote PF progression. Targeting METTL3-mediated m6A RNA methylation and its downstream targets may present innovative therapeutic approaches for the prevention and treatment of PF.
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- 2023
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5. Analysis of Phenotypic Trait Variation in Germplasm Resources of Lycium ruthenicum Murr.
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Rong Yang, Jinpu Li, Haiguang Huang, Xiuhua Wu, Riheng Wu, and Yu’e Bai
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Lycium ruthenicum ,germplasm resources ,phenotypic traits ,genetic variation ,cluster analysis ,Agriculture - Abstract
Exploring the phenotypic trait variation and diversity of Lycium ruthenicum germplasm resources can support selection, breeding, and genetic improvement, enhancing agricultural production. This study collected 213 wild Lycium ruthenicum seedlings from a resource nursery in Alxa League, Inner Mongolia. These seedlings originated from eight sources across four provinces. Using 11 pseudo-qualitative traits and 20 quantitative traits, the phenotypic variation of the germplasm was analyzed. The analysis involved the coefficient of variation, Shannon–Wiener index (H), Simpson’s genetic diversity index (D), principal component analysis, correlation analysis, and Q-type cluster analysis. The results showed that the variation range of 31 phenotypic traits across the 213 Lycium ruthenicum germplasm resources was 17.26% to 105.41%, with an average coefficient of variation of 39.85%. The H and D indexes ranged from 0.18 to 1.58 and 0.20 to 0.75, respectively. For the 11 pseudo-qualitative traits, the H and D ranges were 0.18 to 1.58 and 0.07 to 0.74, with average values of 0.77 and 0.42. For the quantitative traits, the H and D ranges were 0.54 to 1.49 and 0.25 to 0.75, with average values of 1.21 and 0.63. This indicates that Lycium ruthenicum germplasm resources exhibit significant phenotypic diversity, with quantitative traits showing higher diversity than pseudo-qualitative traits. Principal component analysis revealed that the cumulative variance contribution rate of the first 10 principal components was 74.03%, comprehensively reflecting the information of the 31 traits. Q-type cluster analysis grouped the 213 Lycium ruthenicum germplasm resources into six clusters, each with distinct phenotypic characteristics. This analysis also identified the trait characteristics and breeding value of each cluster. The results of this study provide valuable information on the genetic improvement, conservation, and evaluation of Lycium ruthenicum germplasm resources.
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- 2024
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6. O-RADS combined with contrast-enhanced ultrasound in risk stratification of adnexal masses
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Yanyun Shi, Huan Li, Xiuhua Wu, Xiaoqin Li, and Min Yang
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Ultrasound ,O-RADS ,CEUS ,Adnexal Tumor ,Ovarian Cancer ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract Background Ovarian-Adnexal Reporting and Data System (O-RADS) for ultrasound is a lexicon and risk stratification system that includes all risk categories and relevant management recommendation. It has high sensitivity in diagnosing malignant adnexal tumors, but the specificity is lower. Objective To explore the value of O-RADS combined with contrast-enhanced ultrasound (CEUS) in risk stratification of adnexal masses. Methods A retrospective study was performed on 85 patients with 100 adnexal masses that preoperatively underwent conventional ultrasound as well as CEUS examination and obtained the postoperative pathological results. The masses were classified into O-RADS2, 3, 4, and 5 by conventional ultrasound. After contrast enhancement, the classification of O-RADS was adjusted according to CEUS imaging features. The O-RADS 2 and 3 lesions with suspected malignant features like irregular blood vessels or internal inhomogeneous hyperenhancement were upgraded to O-RADS 4, and the O-RADS 4 lesions with the above features were upgraded to O-RADS 5. The O-RADS 4 lesions with suspicious benign angiographic features like a regular vessel, interior hypoenhancement or non-enhancement were downgraded to O-RADS 3; the O-RADS 5 lesions with rim ring-enhancement and interior non-enhancement were downgraded to O-RADS 3. The sensitivity, specificity, accuracy, PPV, NPV, and AUC of the two methods were compared, taking pathological results as the gold standard. Results The sensitivity, specificity, accuracy, PPV, NPV, and AUC of O-RADS and O-RADS combined with CEUS in the diagnosis of malignant adnexal tumors were 96.6%, 66.2%, 75.0%, 53.8%, 97.9%, 0.910 and 96.6%, 91.5%, 93.0%, 82.4%, 98.5%, 0.962, respectively. The specificity, accuracy, PPV, and AUC of O-RADS combined with CEUS were considerably higher than those of O-RADS (P 0.05). Conclusion Combination of O-RADS and CEUS can significantly improve the specificity and PPV in diagnosing malignant adnexal tumors. It seems promising in the clinical application of risk stratification of adnexal masses.
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- 2023
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7. D-DI/PLT can be a prognostic indicator for sepsis
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Xiaojun Zhao, Xiuhua Wu, Yi Si, Jiangang Xie, Linxiao Wang, Shanshou Liu, Chujun Duan, Qianmei Wang, Dan Wu, Yifan Wang, Jijun Chen, Jing Yang, Shanbo Hu, Wen Yin, and Junjie Li
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Sepsis ,Human ,Prognosis ,D-DI/PLT ,ROC curve ,Risk factor ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Aims To investigate the indicators affecting the early outcome of patients with sepsis and to explore its prognostic efficacy for sepsis. Methods We collected clinical data from 201 patients with sepsis admitted to the emergency department of Xijing Hospital between June 2019 and June 2022. The patients were categorized into groups (survival or fatality) based on their 28-day prognosis. The clinical characteristics, biochemical indexes, organ function-related indicators, and disease scores of the patients were analyzed for both groups. Risk factor analysis was conducted for the indicators with significant differences. Results Among the indicators with significant differences between the deceased and survival groups, D-dimer (D-DI), Sequential Organ Failure Assessment (SOFA) score, platelet (PLT), international normalized ratio (INR), and D-DI/PLT were identified as independent risk factors affecting the prognosis of sepsis patients. Receiver operating characteristic (ROC) curves showed that D-DI/PLT (area under the curve (AUC) = 93.9), D-DI (AUC = 89.6), PLT (AUC = 81.3), and SOFA (AUC = 78.4) had good judgment efficacy. Further, Kaplan Meier (K-M) survival analysis indicated that the 28-day survival rates of sepsis patients were significantly decreased when they had high levels of D-DI/PLT, D-DI, and SOFA as well as low PLTs. The hazard ratio (HR) of D-DI/PLT between the two groups was the largest (HR = 16.19). Conclusions D-DI/PLT may be an independent risk factor for poor prognosis in sepsis as well as a clinical predictor of patient prognosis.
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- 2023
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8. Corrigendum to 'A nuclear targeted Dox-aptamer loaded liposome delivery platform for the circumvention of drug resistance in breast cancer' [Biomed. Pharmacother. 117 (2019) 109072]
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Xin Li, Xiuhua Wu, Hongyu Yang, Lin Li, Ziqi Ye, and Yuefeng Rao
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Therapeutics. Pharmacology ,RM1-950 - Published
- 2023
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9. Correction: Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behcet’s disease
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Xiuhua Wu, Zhimian Wang, Jing Shi, Xin Yu, Chaoran Li, Jinjing Liu, Fengchun Zhang, Hua Chen, and Wenjie Zheng
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Diseases of the musculoskeletal system ,RC925-935 - Published
- 2022
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10. Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
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Xiuhua Wu, Zhimian Wang, Jing Shi, Xin Yu, Chaoran Li, Jinjing Liu, Fengchun Zhang, Hua Chen, and Wenjie Zheng
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Behçet’s disease ,Macrophage polarization ,Phagocytosis ,Th1 differentiation ,NF-κB pathway ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Macrophages are key innate immune cells implicated in the pathogenesis of Behçet’s disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization. Methods BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4+T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-κB signaling was examined using western blot to explore the mechanism of macrophage polarization induced by BD serum. Results BD serum-treated macrophages expressed a higher level of CD86, IL-12, and TNF-α and a lower level of CD163, which were compatible with the M1-like phenotype. Furthermore, BD serum-treated macrophages showed enhanced phagocytic capacity and promoted more Th1 cell differentiation. Sixty-one differentially expressed genes were identified between BD and HC serum-treated macrophages and were enriched in NF-κB signaling. BD serum-treated macrophages showed upregulated p-p65 and downregulated IκBα, and NF-κB inhibitor attenuated BD serum-stimulated M1-like phenotype. Conclusions BD serum promoted macrophage polarization toward a proinflammatory M1-like phenotype through NF-κB signaling and potentially facilitated inflammation in BD. M1 polarized macrophages may be a potential therapeutic target for BD.
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- 2022
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11. Neuropsychiatric lupus erythematosus with neurogenic pulmonary edema and anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor limbic encephalitis: a case report
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Rongqi Li, Yingai Wang, Xiuhua Wu, Junping Wang, Wei Wei, and Xin Li
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Systemic lupus erythematosus ,Neuropsychiatric lupus erythematosus ,Neurogenic pulmonary edema ,Anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune inflammatory disease predominantly found in women of child-bearing age. Neurogenic pulmonary edema (NPE) is a recalcitrant complication that occurs after injury to the central nervous system and has an acute onset and rapid progression. Limbic encephalitis is an inflammatory encephalopathy caused by viruses, immune responses, or other factors involving the limbic system. NPE caused by SLE is rare. Case presentation Here, we report a case of a 21-year-old woman with SLE who experienced five episodes of generalized tonic–clonic seizure after headache and dyspnea. Anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) 2 antibody was tested positive in the serum and cerebrospinal fluid. Electrocardiography (EEG) indicated paroxysmal or sporadic medium amplitude theta activity. In addition, chest computed tomography (CT) showed multiple diffuse consolidations and ground-glass opacities. We finally considered a diagnosis of NPE and AMPAR limbic encephalitis. The patient's symptoms improved obviously after methylprednisolone pulse therapy and antiepileptic treatment. Conclusions NPE can be a complication of neuropsychiatric lupus erythematosus (NPSLE). AMPAR2 antibodies may be produced in NPSLE patients, especially in those with high polyclonal IgG antibody titers. More basic and clinical studies are required to confirm these observations and elucidate the pathogenicity of encephalitis-related autoantibodies in SLE patients.
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- 2022
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12. Are serum levels of inflammatory markers associated with the severity of symptoms of bipolar disorder?
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Xiuhua Wu, Zhongcheng Chen, Yingtao Liao, Zhihua Yang, Xiaolin Liang, Nianhong Guan, and Zhaoyu Gan
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bipolar disorder ,symptomatic severity ,inflammatory markers ,stage ,pathophysiology ,Psychiatry ,RC435-571 - Abstract
BackgroundTo explore the relationship between serum levels of inflammatory markers and symptomatic severity of bipolar disorder (BD).Materials and methodsA cross-sectional study was conducted on 126 BD patients with current depressive episode (BDD), 102 BD patients with current mixed or (hypo)manic episode (BDM) and 94 healthy controls (HC). All participants were drug-naïve and had no current active physical illness associated with inflammatory response or history of substance abuse. Fasting serum levels of CRP, leptin (LEP), adiponectin (ADP), visfatin (VIS), TNF-α, IL-2, IL-6, IL-10, IL-17), and monocyte chemoattractant protein-1 (MCP-1) were measured with enzyme-linked immunosorbent assay (ELISA). Symptomatic severity of BD was assessed with HAMD-17 and YMRS. Generalized linear model was used to determine the association between the serum levels of inflammatory markers and symptomatic severity of BD.ResultsThe serum levels of IL-6, IL-10 and IL-17, and the IL-6/IL-10 ratio were significantly lower in mild BDD than in HC. In moderate BDD, the serum levels of MCP, IL-6 and IL-17 were significantly lower than in HC. In severe BDD, the serum level of ADP, MCP-1, IL-10 and IL-17and the IL-17/IL-10 ratio were significantly lower than in HC. The serum levels of TNF-α and the IL-6/IL-10 ratio were significantly higher in mild BDM than in HC. In moderate BDM, the serum level of VIS, IL-2, and IL-17 were significantly higher than in HC, but the IL-6/IL-10 ratio was significantly lower than in control. In severe BDM, the serum levels of IL-6 and IL-17 and the ratios of IL-6/IL-10 and IL-17/IL-10 were significantly lower than in HC, but the neutrophil/lymphocyte ratio was significantly higher than in HC.ConclusionIn BDD, immune-inhibition is persistently predominant, while in mild-to-moderate BDM, immune system is activated but inhibited in severe BDM. The dynamic change of serum inflammatory markers suggests that alteration of peripheral inflammatory markers in BD is state-dependent instead of trait-marked.
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- 2023
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13. Unilateral cervical spinal cord injury induces bone loss and metabolic changes in non-human primates (Macaca fascicularis)
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Xiuhua Wu, Xiaolin Xu, Qi Liu, Jianyang Ding, Junhao Liu, Zhiping Huang, Zucheng Huang, Xiaoliang Wu, Rong Li, Zhou Yang, Hui Jiang, Jie Liu, and Qingan Zhu
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Osteoporosis ,Spinal cord injury ,Bone microarchitecture ,Non-human primates (NHP) ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Background/Objective: The deleterious effects of chronic spinal cord injury (SCI) on the skeleton in rats, especially the lower extremities, has been proved previously. However, the long-term skeletal changes after SCI in non-human primates (NHP) have been scarcely studied. This study aimed to evaluate the bone loss in limbs and vertebrae and the bone metabolic changes in NHP after unilateral cervical spinal cord contusion injury. Methods: Twelve Macaca fascicularis were randomly divided into the SCI (n=8) and the Sham (n=4) groups. The SCI models were established using hemi-contusion cervical spinal cord injury on fifth cervical vertebra (C5), and were further evaluated by histological staining and neurophysiological monitoring. Changes of bone microstructures, bone biomechanics, and bone metabolism markers were assessed by micro-CT, micro-FEA and serological kit. Results: The NHP hemi-contusion cervical SCI model led to consistent unilateral limb dysfunction and potential plasticity in the face of loss of spinal cord. Furthermore, the cancellous bone mass of ipsilateral humerus and radius decreased significantly compared to the contralateral side. The bone volume fraction of humerus and radius were 17.2% and 20.1% on the ipsilateral while 29.0% and 30.1% on the contralateral respectively. Similarly, the thickness of the cortical bone in the ipsilateral forelimbs was significantly decreased, as well as the bone strength of the ipsilateral forelimbs. These changes were accompanied by diminished concentration of osteocalcin and total procollagen type 1 N-terminal propeptide (t-P1NP) as well as increased level of β-C-terminal cross-linking telopeptide of type 1collagen (β-CTX) in serological testing. Conclusions: The present study demonstrated that hemi-SCI induced loss of bone mass and compromised biomechanical performance in ipsilateral forelimbs, which could be indicated by both muscle atrophy and serological changes of bone metabolism, and associated with a consistent loss of large-diameter cells of sensory neurons in the dorsal root ganglia. The Translational potential of this Article: Our study, for the first time, demonstrated the bone loss in limbs and vertebrae as well as the bone metabolic changes in non-human primates after unilateral spinal cord injury (SCI). This may help to elucidate the role of muscle atrophy, serological changes and loss of sensory neurons in the mechanisms of SCI-induced osteoporosis, which would be definitely better compared with rodent models.
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- 2021
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14. Cervical spinal instability causes vertebral microarchitecture change and vertebral endplate lesion in rats
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Qi Liu, Zhou Yang, Yapu Liu, Wei Ji, Zucheng Huang, Junhao Liu, Junyu Lin, Yue Hua, Zhiping Huang, Xiuhua Wu, and Qingan Zhu
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Biomechanical property ,Bone formation ,Intervertebral disc degeneration ,Spinal instability ,Vertebral endplate damage ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Background: The vertebral endplate (VEP) was damaged after spinal instability induced by cervical muscle section (CMS). Whether CMS induces bone formation and mechanical loading change in the vertebra is still obscure. This study was aimed to explore mechanical loading change and endplate damage after CMS. Methods: Forty-eight rats were randomly divided into the CMS group and the sham group. The C6/7 segments were harvested at 4, 8, and 12 weeks after surgery. The microarchitectures of the C6 vertebra and the vertebral endplate lesions and intervertebral disc height of C6/7 were measured by micro–computed tomography. Micro–finite element analysis was used to evaluate biomechanical properties of the C6 vertebra. Bone remodelling of the C6 vertebra and the endplate sclerosis and intervertebral disc degeneration of C6/7 were evaluated by histological and immunohistochemical analyses. Results: CMS significantly induced bone formation of the C6 ventral vertebra and increased the biomechanical properties of mainly the ventral side at 4 weeks, which was gradually rebalanced throughout the rest of the study. CMS also significantly increased protein expression of transforming growth factor-β1 (TGF-β1) and phosphorylated small mothers against decapentaplegic (pSmad)2/3 at 4 weeks. Moreover, tartrate-resistant acid phosphatase staining showed that osteoclast-positive cells were slightly in number decreased at 4 weeks, but were obviously increased at 8 weeks. The VEP of the ventral side was abraded earlier followed by calcification in situ later after CMS, consistent with the biomechanical enhancements observed. The degree of endplate degeneration was aggravated with time. Finally, CMS decreased intervertebral disc height and increased disc degeneration scores with time. Conclusions: Spinal instability induced by CMS increases bone mass and biomechanical loading of the ventral side of vertebra in the early stage, which might initiate VEP damage and cause intervertebral disc degeneration. The translational potential of this article: Our study indicates that vertebral trabecular changes may involve in intervertebral disc degeneration induced by spinal instability. This may help to elucidate the mechanisms by which disc degeneration occur.
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- 2020
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15. Rapid cycling bipolar disorder is associated with antithyroid antibodies, instead of thyroid dysfunction
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Zhaoyu Gan, Xiuhua Wu, Zhongcheng Chen, Yingtao Liao, Yingdong Wu, Zimeng He, Zhihua Yang, and Qi Zhang
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Bipolar disorder ,Rapid cycling ,Thyroid dysfunction ,Antithyroid peroxidase antibodies ,Anti-thyroiglobulin antibodies ,Psychiatry ,RC435-571 - Abstract
Abstract Background Conclusions regarding the association between antithyroid antibodies or thyroid dysfunction and rapid cycling bipolar disorder (RCBD) have been conflicting. Previous studies suggest that the impact of antithyroid antibodies on mental wellbeing seems to be independent of thyroid function. Here, we investigated their independent association with RCBD in a large, well-defined population of bipolar disorder (BD). Methods Fast serum levels of free thyroxine (FT4), free triiodothyronine (FT3), thyroid Stimulating Hormone (TSH), TPO-abs and Tg-abs were simultaneously measured in 352 patients with BD. Clinical features of BD were collected through semi-structural interview conducted by trained interviewers with background of psychiatric education. Results Neither hypothyroidism nor hyperthyroidism was significantly associated with RCBD. Both TPO-abs and Tg-abs were significantly related to RCBD, even after controlling for gender, age, marriage status, education, antidepressants treatment, comorbidity of thyroid diseases, and thyroid function (serum levels of FT3, FT4 and TSH). Although TPO-abs and Tg-abs were highly correlated with each other, binary logistic regression with forward LR selected TPO-abs, instead of Tg-abs, to be associated with RCBD. TPO-abs was significantly, independently of Tg-abs, associated with hyperthyroidism, while Tg-abs was marginally significantly related to hypothyroidism at the presence of TPO-abs. Conclusion TPO-abs might be treated as a biomarker of RCBD. Further exploring the underlying mechanism might help understand the nature of RCBD and find out new treatment target for it.
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- 2019
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16. Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion
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Yapu Liu, Qi Liu, Zhou Yang, Rong Li, Zhiping Huang, Zucheng Huang, Junhao Liu, Xiuhua Wu, Junyu Lin, Xiaoliang Wu, and Qingan Zhu
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trihydroxyethyl rutin ,neuroprotection ,spinal cord injury ,microvascular ,electrophysiology ,behavior ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Objective: To investigate the neuroprotective effects of trihydroxyethyl rutin in rats with cervical spinal cord hemi-contusion.Methods: Adult male Sprague–Dawley rats were subjected to hemi-contusion at a stroke depth of 1.2 mm, and then intraperitoneally injected with 50 or 100 mg/kg trihydroxyethyl rutin per day for 12 weeks (T50 and T100 groups, respectively). Changes in somatosensory evoked potentials (SEPs), motor evoked potentials (MEPs), and behavior were continuously monitored. At 12 weeks post-injury, immunohistochemical staining was performed to assess changes in cervical spinal cord microvascular morphology. Magnetic resonance imaging (MRI) scans were performed to examine end-stage injury in the cervical spinal cord, and Eriochrome cyanine-stained slices of spinal cord tissue were evaluated for injury.Results: There were no significant differences in biomechanical parameters among the spinal cord injury, T50 and T100 rat groups. At 3 days-post-injury, there was a significant decrease in grip strength. At 12 weeks post-injury, grip strength recovery was significantly better in the T50 and T100 groups than in the injury group. Compared with the injury group, the total limb placement frequency was significantly higher in the T50 group at 2, 4, 6, 10, and 12 weeks post-injury and in the T100 group at 2, 6, 8, and 10 weeks post-injury. Ipsilateral SEPs and MEPs were dynamic, increasing in latency and decreasing in amplitude in the injury compared with sham group. MRI scanning demonstrated that the coronal, sagittal, and transversal lesion areas were smaller in the T50 and T100 groups than in the injury group. Microvascular density showed a greater reduction in the injury group compared with the T50 and T100 groups. Eriochrome cyanine staining showed that the ipsilateral side, residual parenchyma, and gray matter areas were larger in the T50 and T100 groups than in the injury group.Conclusion: Trihydroxyethyl rutin exhibits robust neuroprotective effects, improving limb motor function and nerve electrophysiological parameters after spinal cord injury, maintaining microvascular density, and reducing the area of injury and degree of demyelination.
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- 2021
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17. Depression and anxiety symptoms to COVID-19 outbreak among the public, medical staff and patients during the initial phase of the pandemic: an online questionnaire survey by a WeChat Mini Program
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Qi Zhu, Jiong Tao, Xianglan Wang, Xiuhua Wu, Chongbang Zhao, Weirui Yang, Jinbei Zhang, and Nianhong Guan
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Medicine - Abstract
Objectives To survey anxiety and depression symptoms to COVID-19 outbreak in the public, medical staff and patients during the initial phase of the pandemic.Design Cross-sectional online survey administered through WeChat Mini Program using Chinese versions of Zung Self-rating Depression Scale and Zung Self-rating Anxiety Scale.Setting Guangzhou, China.Participants 47 378 public, 1512 medical staff and 125 patients with COVID-19.Results Higher rates of depression (47.8%) and anxiety symptoms (48.7%) were shown by patients who were screened positive compared with those of the public (35.6%, 25.7%) or medical staff (15.4%, 13.3%). The professional identity of a nurse, conditions of ‘with an infected family member’ and ‘working at the frontline’ were risk factors to depression or anxiety symptoms for the medical staff. Younger age, lower educational level, female and not having adequate masks were the risk factors for the public.Conclusion The COVID-19 outbreak increased people’s depression or anxiety emotion responses, which varied extensively among the patients, public and medical staff.
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- 2021
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18. Diagnostic value of the interferon-γ release assay for tuberculosis infection in patients with Behçet’s disease
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Xiuhua Wu, Pang Chen, Wei Wei, Mengyu Zhou, Chaoran Li, Jinjing Liu, Lidan Zhao, Lifan Zhang, Yan Zhao, Xiaofeng Zeng, Xiaoqing Liu, and Wenjie Zheng
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Behçet’s disease ,Tuberculosis ,Interferon-γ release assay ,T-SPOT.TB ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background To investigate the diagnostic value of the interferon-γ release assay (IGRA) for detecting tuberculosis (TB) infection in patients with Behçet’s disease (BD). Methods We retrospective analyzed the data collected from 173 BD patients hospitalized between 2010 and 2015. Ninety-nine healthy volunteers were enrolled as a control group. IGRA was performed using T-SPOT.TB. The diagnosis of active TB (ATB) was based on clinical, radiological, microbiological, histopathological information and the response to anti-TB therapy. Latent TB (LTB) infection was defined as asymptomatic patients with positive T-SPOT.TB. Results TB infection was documented in 59 BD patients (34.1%). The sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio and negative likelihood ratio of T-SPOT.TB for the diagnosis of ATB were 88.9%, 74.8%, 29.1%, 98.3%, 3.53 and 0.15, respectively. The receiver-operating-characteristic curve demonstrated that spot-forming cells (SFCs) of 70/106 PBMC was the optimal cutoff for diagnosing ATB, with an area under the curve of 0.891. Furthermore, the median SFCs in ATB group was significantly higher than those in LTB infection (466/106 PBMC vs. 68/106 PBMC, p = 0.007) or previous TB infection (466/106 PBMC vs. 96/106 PBMC, p = 0.018). A significant discrepancy between T-SPOT.TB and tuberculin skin test was noted (kappa coefficient = 0.391, p = 0.002). Conclusions T-SPOT.TB, an IGRA, may assist in the diagnosis of ATB in BD patients, and the higher SFCs suggest ATB in BD patients.
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- 2019
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19. Case Report: Paliperidone Palmitate in the Management of Bipolar I Disorder With Non-compliance
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Kanglai Li, Yingtao Liao, Zhihua Yang, Caishuang Yang, Minhua Chen, Xiuhua Wu, and Zhaoyu Gan
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bipolar disorder ,treatment ,long-acting injectable antipsychotics ,once-monthly paliperidone palmitate ,non-compliance ,Psychiatry ,RC435-571 - Abstract
Background: Medication non-adherence is prevalent in patients with bipolar disorder (BD). Long-acting injectable antipsychotics (LAIAs) are widely used to improve compliance with treatment. This study aimed to illustrate the effectiveness, compliance, and safety profile of once-monthly paliperidone palmitate (PP1M), a novel therapeutic LAIA, in the management of bipolar I disorder (BDI).Method: A prospective follow-up was arranged to 11 BDI patients who were prescribed PP1M as monotherapy or adjunctive treatment. Severity of symptoms, disturbing behavior, status of employment, 17-item Hamilton Depression Rating Scale (HAMD-17), and Young Mania Rating Scale (YMRS) were evaluated at the baseline and the endpoint of follow-up. Clinical Global Impression—Bipolar Disorder—Severity of Illness Scale (CGI-BP) and Treatment Emergent Symptom Scale (TESS) were measured at each injection of PP1M. Compliance, relapse or switch, and new hospitalization were monitored through the period of follow-up.Results: The median duration of treatment was 14 months, ranging from 5 to 22 months. The scores (mean ± standard deviation) of HAMD-17, YMRS, and CGI-BP generally decreased from the baseline (16.1 ± 10.3, 30.9 ± 12.6, 5.3 ± 0.7) to the endpoint (7.4 ± 5.7, 3.7 ± 3.2, 2.3 ± 0.7). No disturbing behavior was detected at the endpoint. Neither new hospitalization nor manic/mixed episode occurred during treatment, whereas mild to moderate depressive episodes were reported in three cases. The status of employment of 10 participants (90.9%) was improved, and no new safety concern was detected.Conclusion: PP1M might offer a new valid treatment option in the long-term management of BDI, especially for those with poor compliance with oral medication. However, more studies are needed to further justify such role.
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- 2021
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20. Rapamycin Preserves Neural Tissue, Promotes Schwann Cell Myelination and Reduces Glial Scar Formation After Hemi-Contusion Spinal Cord Injury in Mice
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Junhao Liu, Ruoyao Li, Zucheng Huang, Junyu Lin, Wei Ji, Zhiping Huang, Qi Liu, Xiaoliang Wu, Xiuhua Wu, Hui Jiang, Yongnong Ye, and Qingan Zhu
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autophagy ,spinal cord injury ,rapamycin ,Schwann cells ,neuroprotection ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Protecting white matter is one of the key treatment strategies for spinal cord injury (SCI), including alleviation of myelin loss and promotion of remyelination. Rapamycin has been shown neuroprotective effects against SCI and cardiotoxic effects while enhancing autophagy. However, specific neuroprotection of rapamycin for the white matter after cervical SCI has not been reported. Therefore, we aim to evaluate the role of rapamycin in neuroprotection after hemi-contusion SCI in mice. Forty-six 8-week-old mice were randomly assigned into the rapamycin group (n = 16), vehicle group (n = 16), and sham group (n = 10). All mice of the rapamycin and vehicle groups received a unilateral contusion with 1.2-mm displacement at C5 followed by daily intraperitoneal injection of rapamycin or dimethyl sulfoxide solution (1.5 mg⋅kg–1⋅day–1). The behavioral assessment was conducted before the injury, 3 days and every 2 weeks post-injury (WPI). The autophagy-related proteins, the area of spared white matter, the number of oligodendrocytes (OLs) and axons were evaluated at 12 WPI, as well as the glial scar and the myelin sheaths formed by Schwann cells at the epicenter. The 1.2 mm contusion led to a consistent moderate–severe SCI in terms of motor function and tissue damage. Rapamycin administration promoted autophagy in spinal cord tissue after injury and reduced the glial scar at the epicenter. Additionally, rapamycin increased the number of OLs and improved motor function significantly than in the vehicle group. Furthermore, the rapamycin injection resulted in an increase of Schwann cell-mediated remyelination and weight loss. Our results suggest that rapamycin can enhance autophagy, promote Schwann cell myelination and motor function recovery by preserved neural tissue, and reduce glial scar after hemi-contusive cervical SCI, indicating a potential strategy for SCI treatment.
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- 2021
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21. Inhibitory Effect of Piceatannol on Streptococcus suis Infection Both in vitro and in vivo
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Guizhen Wang, Yawen Gao, Xiuhua Wu, Xiue Gao, Min Zhang, Hongmei Liu, and Tianqi Fang
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Streptococcus suis ,piceatannol ,suilysin ,virulence ,molecular modeling ,Microbiology ,QR1-502 - Abstract
Suilysin (SLY) plays a critical role in Streptococcus suis infections making it an ideal target to the combat infection caused by this pathogen. In the present study, we found that piceatannol (PN), a natural compound, inhibits pore-formation by blocking the oligomerization of SLY without affecting the growth of S. suis and the expression of SLY. Furthermore, PN alleviated the J774 cell damage and the expression of the inflammatory cytokine tumor necrosis factor-α (TNF-α) and interleukin-1α (IL-1β) induced by S. suis in vitro. The computational biology and biochemistry results indicated that PN binds to the joint region of D2 and D4 in SLY, and Asn57, Pro58, Pro59, Glu76, Ile379, Glu380, and Glu418 were critical residues involved in the binding. The binding effect between PN and SLY hindered the SLY monomers from forming the oligomers, thereby weakening the hemolytic activity of SLY. This mechanism was also verified by hemolysis analysis and analysis of KA formation after site-specific mutagenesis. Furthermore, PN protected mice from S. suis infections by reducing bacterial colony formation and the inflammatory response in target organs in vivo. These results indicate that PN is a feasible drug candidate to combat S. suis infections.
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- 2020
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22. Neuroprotective Effect of Ketone Metabolism on Inhibiting Inflammatory Response by Regulating Macrophage Polarization After Acute Cervical Spinal Cord Injury in Rats
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Junyu Lin, Zucheng Huang, Junhao Liu, Zhiping Huang, Yapu Liu, Qi Liu, Zhou Yang, Ruoyao Li, Xiuhua Wu, Zhe Shi, Qingan Zhu, and Xiaoliang Wu
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spinal cord injury ,ketone metabolism ,ketogenic diet ,inflammation ,macrophage ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
ObjectiveTo investigate the effects of ketogenic metabolism on macrophage polarization, inflammation inhibition, and function recovery after acute spinal cord injury (SCI) in rats.MethodsSixty-four adult male Sprague–Dawley rats were randomly and equally divided into sham, standard diet (SD), ketone diet (KD), and 1, 3-butanediol (BD) groups. All animals underwent C5 unilateral laminectomy, whereas the SD, KD, and BD groups underwent C5 spinal cord hemi-contusion. The impact rod with a diameter of 1.5 mm was aligned 22.5° to the left and 1.4 mm to the midline, and then triggered to deliver a set displacement of 1.5 mm at a speed of 100 mm/s. The gene expression of inflammatory factors as well as the protein expression of inducible nitric oxide synthase, arginase-1, and inflammatory factors were measured at 1 week post-injury. Serum ketone and behavior were evaluated every second week for 12 weeks. Then, histological analyses of the gray and white matter at the epicenter were conducted at 12 weeks post-injury.ResultsThe serum ketone levels of the KD and BD groups were significantly increased when compared with the SD group. The gene and protein expression of TNF-α and IL-1β tended to increase after the SCI, but were inhibited in the KD and BD groups. The protein expression of inducible nitric oxide synthase, marker of M1 macrophage, was inhibited in the KD and BD groups; on the other hand, the expression of arginase-1, marker of M2 macrophage, was boosted in the KD and BD groups. The usage of the ipsilateral forelimb was higher in the KD group than in the SD group. The hemi-contusive injury resulted in an obvious ipsilateral lesion area at the epicenter, and there was no significant difference between groups regarding the lesion size. However, the spared gray matter area was significantly greater in the KD group than in the SD and BD groups.ConclusionThe present study suggests that ketogenic metabolism promotes macrophage polarization to M2, inhibits an inflammatory response, and alleviates the loss of gray matter after SCI. A higher ketone level, such as that induced by the ketogenic diet, seems to benefit function recovery after SCI.
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- 2020
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23. Liver Injury in Critically Ill and Non-critically Ill COVID-19 Patients: A Multicenter, Retrospective, Observational Study
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Saiping Jiang, Rongrong Wang, Lu Li, Dongsheng Hong, Renping Ru, Yuefeng Rao, Jing Miao, Na Chen, Xiuhua Wu, Ziqi Ye, Yunzhen Hu, Minghua Xie, Minjuan Zuo, Xiaoyang Lu, Yunqing Qiu, and Tingbo Liang
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Incidence ,risk factors ,liver injury ,COVID-19 ,disease severity ,Medicine (General) ,R5-920 - Abstract
Background: Liver injury commonly occurs in patients with COVID-19. There is limited data describing the course of liver injury occurrence in patients with different disease severity, and the causes and risk factors are unknown. We aim to investigate the incidence, characteristics, risk factors, and clinical outcomes of liver injury in patients with COVID-19.Methods: This retrospective observational study was conducted in three hospitals (Zhejiang, China). From January 19, 2020 to February 20, 2020, patients confirmed with COVID-19 (≥18 years) and without liver injury were enrolled and divided into non-critically ill and critically ill groups. The incidence and characteristics of liver injury were compared between the two groups. Demographics, clinical characteristics, treatments, and treatment outcomes between patients with or without liver injury were compared within each group. The multivariable logistic regression model was used to explore the risk factors for liver injury.Results: The mean age of 131 enrolled patients was 51.2 years (standard deviation [SD]: 16.1 years), and 70 (53.4%) patients were male. A total of 76 patients developed liver injury (mild, 40.5%; moderate, 15.3%; severe, 2.3%) with a median occurrence time of 10.0 days. Critically ill patients had higher and earlier occurrence (81.5 vs. 51.9%, 12.0 vs. 5.0 days; p < 0.001), greater injury severity (p < 0.001), and slower recovery (50.0 vs. 61.1%) of liver function than non-critically ill patients. Multivariable regression showed that the number of concomitant medications (odds ratio [OR]: 1.12, 95% confidence interval [CI]: 1.05–1.21) and the combination treatment of lopinavir/ritonavir and arbidol (OR: 3.58, 95% CI: 1.44–9.52) were risk factors for liver injury in non-critically ill patients. The metabolism of arbidol can be significantly inhibited by lopinavir/ritonavir in vitro (p < 0.005), which may be the underlying cause of drug-related liver injury. Liver injury was related to increased length of hospital stay (mean difference [MD]: 3.2, 95% CI: 1.3–5.2) and viral shedding duration (MD: 3.0, 95% CI: 1.0–4.9).Conclusions: Critically ill patients with COVID-19 suffered earlier occurrence, greater injury severity, and slower recovery from liver injury than non-critically ill patients. Drug factors were related to liver injury in non-critically ill patients. Liver injury was related to prolonged hospital stay and viral shedding duration in patients with COVID-19.Clinical Trial Registration: World Health Organization International Clinical Trials Registry Platform, ChiCTR2000030593. Registered March 8, 2020.
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- 2020
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24. Protective effects of Corbrin Capsule against permanent cerebral ischemia in mice
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Jiaying Wu, Wenping Yan, Xiuhua Wu, Dongsheng Hong, Xiaoyang Lu, and Yuefeng Rao
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Corbrin Capsule ,Cerebral ischemia ,MCAO ,Neuroprotection ,Antioxidation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Corbrin Capsule is a traditional Chinese patent medicine with the main component of fermentative cordyceps fungus powder (Cs-C-Q80). The indications of Corbrin Capsule include chronic renal insufficiency, chronic obstructive pulmonary (COPD), pulmonary fibrosis, and chronic bronchitis. However, the effects of Corbrin Capsule on acute cerebral ischemia are still unclear. The objective of this study was to explore the preventive effect of Corbrin Capsule in permanent and transient middle cerebral artery occlusion (MACO) mice model. Male C57BL/6 mice were given Corbrin of 0.04, 0.2 and 1 mg/kg by gavage once a day for 3, 7 or 14 days and then subjected to pMCAO or tMCAO. Infarct volumes, neurological deficit score, ATP concentration, SOD activity and MDA content were assessed. Results showed that prolonged pretreatment with Corbrin (1.0 mg/kg) to 7 days or more effectively ameliorated brain infarct and neurological scores in pMCAO mice. Shorter (3 days) or without pretreatment of Corbrin was invalid, suggesting a pretreatment time window. The ATP concentration was significantly increased with effective Corbrin pretreatments in ischemic brains, while the content of MDA sharply decreased in Corbrin groups. In tMCAO mice, Corbrin showed no neuroprotection even with pretreatment. In conclusion, long-term pre-administration of Corbrin Capsule is necessary for its anti-cerebral ischemic effects, and the underlying mechanisms might be associated with increase of ATP concentration and the anti-inflammatory effects in ischemic brain tissue.
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- 2020
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25. Metabolomic alterations associated with Behçet’s disease
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Wenjie Zheng, Xiuhua Wu, Maryam Goudarzi, Jing Shi, Wei Song, Chaoran Li, Jinjing Liu, Hua Chen, Xuan Zhang, Xiaofeng Zeng, and Heng-Hong Li
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Behçet’s disease ,Autoinflammatory disease ,Biomarker ,Metabolomics ,Lipidomics ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background The diagnosis of Behçet’s disease (BD) remains challenging due to the lack of diagnostic biomarkers. This study aims to identify potential serum metabolites associated with BD and its disease activity. Methods Medical records and serum samples of 24 pretreated BD patients, 12 post-treated BD patients, and age-matched healthy controls (HC) were collected for metabolomics and lipidomics profiling using UPLC-QTOF-MS and UPLC-QTOF-MSE approaches. Additionally, serum samples from an independent cohort of BD patients, disease controls including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), Takayasu’s arteritis (TA), Crohn’s disease (CD) patients, and HC were collected for further validation of two potential biomarkers using UPLC-QTOFMS analysis. Results Unsupervised principal component analysis (PCA) showed a clear separation of metabolomics profiles of BD patients from HC. Statistical analysis of the data revealed differential metabolites between BD patients and HC. The serum levels of some phosphatidylcholines (PCs) were found to be significantly lower in BD patients, while the levels of several polyunsaturated fatty acids (PUFAs) were increased markedly in the BD group compared with HC. Furthermore, the serum level of two omega-6 PUFAs, linoleic acid (LA) and arachidonic acid (AA), were dramatically decreased in patients with remission. A validation cohort confirmed that the serum LA and AA levels in BD patients were significantly higher than those in HC and patients with RA, SLE, TA, and CD. In addition, receiver operating characteristic (ROC) analysis indicated good sensitivity and specificity. Conclusions The serum metabolomics profiles in BD patients are altered. Serum LA and AA are promising diagnostic biomarkers for BD.
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- 2018
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26. Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish
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Shaoyang Zhao, Jianhong Xia, Xiuhua Wu, Leilei Zhang, Pengtao Wang, Haiyun Wang, Heying Li, Xiaoshan Wang, Yan Chen, Jean Agnetti, Yinxiong Li, Duanqing Pei, and Xiaodong Shu
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Science - Abstract
The functions of the class III PI3-kinase (PIK3C3) in gut homeostasis and innate immunity are poorly understood. Here the authors show that PIK3C3-deficient zebrafishes develop intestinal injury and inflammation due to mislocalization of cell junction proteins.
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- 2018
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27. A nuclear targeted Dox-aptamer loaded liposome delivery platform for the circumvention of drug resistance in breast cancer
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Xin Li, Xiuhua Wu, Hongyu Yang, Lin Li, Ziqi Ye, and Yuefeng Rao
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Nuclear uptake ,Drug-resistant cancer therapy ,Aptamer ,Nucleolin ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The development of multidrug resistance (MDR) has become an increasingly serious problem in cancer therapy. The cell membrane overexpression of P-glycoprotein (P-gp), which can actively efflux various anticancer drugs in the cytoplasm from the cell, is a major mechanism of MDR. Nuclear-targeted nanoparticle drug delivery system, which enables intranuclear release of anticancer drugs, is expected to address this challenge. In this study, based on nucleolin’s active transport property to the nucleus and its affinity with aptamer, we developed a nuclear-targeted delivery system to circumvention of drug resistance in breast cancer (MCF-7/Adr). Dox·HCl inserted in the aptamer AS1411 (Ap-Dox) was encapsulated in the aqueous interior of liposome (Lip(Ap-Dox)). In vitro studies showed that after the Lip(Ap-Dox) diffusing into MCF-7/Adr cells, Ap-Dox complex bound with nucleolin strongly and eventually entered the cell nuclei. By using this drug delivery system, Dox·HCl can efficiently accumulated in the nuclei to effectively kill the cancer cells.
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- 2019
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28. The Clinical Features, Risk Factors, and Outcome of Aneurysmal Lesions in Behcet’s Disease
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Jiaxin Zhou, Jing Shi, Jinjing Liu, Luxi Sun, Lu Li, Chaoran Li, Xiuhua Wu, Yining Wang, Xinping Tian, Xiaofeng Zeng, Yi Liu, Wenjie Zheng, and Yan Zhao
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Objective. To investigate the clinical features and potential risk factors of aneurysmal lesions in Behcet’s disease (BD). Methods. We retrospectively reviewed the clinical data of BD patients with aneurysmal lesions in our institute from 1997 to 2017 and compared them with 207 BD patients without aneurysmal lesions. The treatment and outcome of these patients were also analyzed. Results. Sixty-nine patients were included with 117 aneurysmal lesions. The average period between BD onset and diagnosis of aneurysmal lesion was 5.4±5.5 years. Thirty-three patients (47.8%) had multiple aneurysmal lesions. Ten patients developed 20 pulmonary artery aneurysms alone. For the other 97 aortic and/or peripheral artery aneurysms in 59 patients, the most commonly affected vessels were abdominal aorta (27/97, 27.8%), coronary artery (10/97, 10.3%), and superficial femoral artery (8/97, 8.2%). Multivariate analysis revealed pathergy reaction (OR=3.78 (1.70-8.41)), arterial stenosis or occlusion (OR=44.12 (11.56-168.35)), and arterial thrombosis (OR=9.27 (2.33-36.93)) as independent predictors of aneurysmal lesions in BD. With a mean follow-up of 5.5±4.0 years, 40 patients (58.0%) achieved clinical improvements, 15 patients (21.7%) relapsed, and 10 patients (14.5%) died. The respective estimated cumulative 1- and 5-year relapse-free rates were 91.3% and 76.3%, and the respective estimated 1- and 5-year survival rates were 95.0% and 87.2%. Conclusion. Aneurysmal lesions are severe complications in BD. Pathergy reaction, arterial stenosis or occlusion, and arterial thrombosis are the risk factors of aneurysmal lesions in BD. Achieving BD remission and performing surgical or interventional procedures are both important in the treatment of these patients.
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- 2019
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29. Some New Intrinsic Topologies on Complete Lattices and the Cartesian Closedness of the Category of Strongly Continuous Lattices
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Xiuhua Wu, Qingguo Li, and Dongsheng Zhao
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Mathematics ,QA1-939 - Abstract
We prove some new characterizations of strongly continuous lattices using two new intrinsic topologies and a class of convergences. Lastly we show that the category of strongly continuous lattices and Scott continuous mappings is cartesian closed.
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- 2013
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30. Topiramate use in a pediatric patient with comorbid bipolar disorder and trichotillomania: A 3-year follow-up.
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Ting Yang, Xiaolin Liang, Xiuhua Wu, Tong Li, Youzhen Lin, and Zhaoyu Gan
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BIPOLAR disorder ,CHILD patients ,COMPULSIVE hair pulling ,COMORBIDITY ,TOPIRAMATE - Abstract
Trichotillomania (TTM) is an intractable and chronic mental disorder that causes significant distress or functional impairments in various life domains. Most individuals with trichotillomania have other comorbid diagnoses. Bipolar disorder (BD) is one of the most common comorbid conditions. Up to date, no FDA-approved drugs for TTM are available, not to mention children and adolescent patients with TTM and BD. Here, we present a case of an 8-year-old child with a long history of episodic TTM and bipolar disorder who was effectively treated with topiramate in a 3-year follow-up. [ABSTRACT FROM AUTHOR]
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- 2024
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31. A Novel Semi‐Cannulated Screw Enhanced Bone Cement Augmentation and Pullout Strength in Posterior Cervical Lateral Mass Screw Fixations: An In Vitro Biomechanical and Morphological Study
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Canyang, Huang, primary, Weidong, Chen, additional, Zhiping, Huang, additional, Xiuhua, Wu, additional, Minghui, Zheng, additional, Haihong, Huang, additional, Qingan, Zhu, additional, and Dongbin, Qu, additional
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- 2023
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32. Analysis of the clinical features of antisynthetase syndrome: a retrospective cohort study in China
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Ruoming Wang, Yin Zhao, Fumin Qi, Xiuhua Wu, Yingai Wang, Yong Xu, Yanan Wu, Na Zhang, Hou Hou, WenWen Sun, Xin Li, and Wei Wei
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Rheumatology ,General Medicine - Abstract
To summarize the clinical, serological, and radiological characteristics of anti-synthetase syndrome (ASS) patients with different anti-aminoacyl-tRNA synthase antibody.Retrospective analysis was performed based on the clinical data of 88 patients diagnosed with ASS in Tianjin Medical University General Hospital from January 2015 to December 2020. The clinical data included general conditions, serological indexes, high-resolution CT (HRCT) characteristics, and pulmonary function manifestations.Among the 88 patients, there were 17 males and 71 females. The anti-synthetase antibodies included anti-Jo-1 (n = 42), anti-PL-7 (n = 14), anti-PL-12 (n = 9), anti-EJ (n = 20), and anti-OJ (n = 3) antibodies. The most common clinical manifestations of ASS patients were interstitial lung disease (ILD) (90%, 79/88), followed by myositis (79.5%, 70/88), arthritis (50%, 44/88), and rash (50%, 44/88). The frequency of arthritis in the anti-Jo-1 antibody-positive group was higher than that of the anti-PL-7 and anti-EJ antibody groups (P = 0.004, P = 0.002, respectively). The frequency of Gottron's sign in the anti-PL-7 antibody positive group was higher than that of the anti-Jo-1 and anti-PL-12 antibody-positive groups (P = 0.006, P = 0.04). Isolated arthritis was the most frequent initial symptoms in anti-Jo-1 antibody-positive group (47.6%, 20/42), while isolated ILD was most frequent in patients with anti-EJ antibody (50%, 10/20), and isolated myositis in patients carrying anti-OJ (66.7%, 2/3). There were only 32 cases (36.4%) with the typical clinical triad (myositis, arthritis, ILD). In our cohort, 79 patients (90%) were complicated with ILD. Meanwhile, 7 out of 79 cases were classified into rapid progressive group with 6 cases (85.7%) carrying anti-Ro-52 antibody. The probability of reticular and honeycombing shadow in HRCT of patients with anti-EJ antibody positive was higher than that of other groups (P 0.05).ILD, myositis, and arthritis were the most common clinical manifestations in patients with ASS. Different antibody-positive patients have different initial symptoms. Patients with isolated arthritis, myositis, and ILD should be vigilant of ASS. The complication of anti-Ro-52 antibody in ASS patients was associated with rapidly progressive pulmonary interstitial disease. Patients with positive anti-EJ antibodies tend to have ILD as the first symptom, and with high occurrence of ILD, the HRCT showed more serious patterns, suggesting the correlation between anti-EJ antibodies and ILD. Key Points • Analyzing specific clinical manifestations in ASS patients with different ARS antibodies can raise awareness of the disease and reduce misdiagnosis. • Anti-EJ antibodies were correlated with ILD. • Anti-Ro-52 antibodies may correlate with RP-ILD in ASS patients.
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- 2022
33. SMPDL3B contributes to gastric adenocarcinoma cells progression by promoting the infiltration of M2 macrophages.
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Li SU, Jian HAO, Na ZHANG, Shan WU, Xiuhua WU, and Wei WEI
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SPHINGOMYELINASE ,GASTRECTOMY ,ADENOCARCINOMA ,MACROPHAGES ,GASTRIC diseases - Abstract
Background/aim: The common disease gastric adenocarcinoma (GAC) has a high morbidity and mortality, so there is an urgent need for research to explore new diagnostic markers and therapeutic targets. This investigation was carried out to investigate the expression of sphingomyelin phosphodiesterase acid-like 3b (SMPDL3B) in GAC and its effects on tumor progression. Materials and methods: Samples were collected from patients who underwent radical gastrectomy from January 2021 to December 2022. Along with the normal gastric epithelial cell lines GES-1 and SGC-7901, the AGS, MGC-803, and MSN-45 human gastric cancer cell lines were used to confirm SMPDL3B expression. RT-qPCR, Western blot, immunohistochemical, cell proliferation, assay of wound healing, transwell migration assay, invasion assay, flow cytometry, and immune evaluation experiments were carried out. Results: SMPDL3B was found to be substantially expressed in GAC, and this condition has a bad prognosis. By establishing SMPDL3B knockdown and overexpression of GAC cell lines, this study confirmed that SMPDL3B promoted tumor cell proliferation, migration, and invasion. Additional bioinformatics research revealed a connection between SMPDL3B and immune cell infiltration in the GAC immunological microenvironment, which enhanced tumor cell proliferation by promoting the infiltration content of M2 macrophages. Conclusion: This study determined the function of SMPDL3B for the clinical diagnosis, prediction, and novel management of GAC. [ABSTRACT FROM AUTHOR]
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- 2023
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34. Developing and designing of a new passive aerobic exercise bed.
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Xiuhua Wu and Chunfeng Huang
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- 2015
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35. Clinicopathological characteristics and associated factors of idiopathic membranous nephropathy with hyperuricemia: a single-centered cross-sectional study
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Dong Liang, Hui Zhang, Qianyu Lin, Xiuhua Wu, Min Yang, Hua Dong, Yaning Wang, Zhenmin Chen, Yunqi Liu, and Xiaomin Zhang
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Nephrology ,Urology - Published
- 2023
36. Dimensionality reduction of hypergraph information system.
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Tian Yang and Xiuhua Wu
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- 2014
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37. Identifying Candidate Genes Associated with Sporadic Amyotrophic Lateral Sclerosis via Integrative Analysis of Transcriptome-Wide Association Study and Messenger RNA Expression Profile
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Ping Li, Shiqiang Cheng, Yan Wen, Bolun Cheng, Li Liu, Xiuhua Wu, Xiang Ao, Zucheng Huang, Congrui Liao, Shaen Li, Feng Zhang, and Zhongmin Zhang
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Cellular and Molecular Neuroscience ,Cell Biology ,General Medicine - Abstract
Amyotrophic lateral sclerosis, a fatal neurodegeneration disease affecting motor neurons in the brain and spinal cord, is difficult to diagnose and treat. The objective of this study is to identify novel candidate genes related to ALS. Transcriptome-wide association study of ALS was conducted by integrating the genome-wide association study summary data (including 1234 ALS patients and 2850 controls) and pre-computed gene expression weights of different tissues. The ALS-associated genes identified by TWAS were further compared with the differentially expressed genes detected by the mRNA expression profiles of the sporadic ALS. Functional enrichment and annotation analysis of identified genes were performed by an R package and the functional mapping and annotation software. TWAS identified 761 significant genes (P
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- 2022
38. IMPACTO DEL ENTRENAMIENTO POR INTERVALOS DE ALTA INTENSIDAD EN LA RESISTENCIA DE JUGADORES DE VOLEIBOL
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Xiuhua Wu
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Endurance Training ,Volleyball ,Treinamento de Endurance ,Voleibol ,Treinamento Intervalado de Alta Intensidade ,Entrenamiento de Intervalos de Alta Intensidad ,Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,High-Intensity Interval Training ,Entrenamiento de Resistencia - Abstract
Introduction: The high-intensity interval training method is believed to improve sports endurance in volleyball players due to its characteristic of alternating short periods of intense aerobic exercise and recovery. Objective: Analyze the impact of high-intensity interval training on sports endurance in volleyball players aged 18 to 19 years old. Methods: We selected 16 male volleyball athletes, and divided them into control and experimental groups. A high-intensity interval training protocol was added to the experimental group, while the control group remained with standard training. The indices of the effect of intensive interval training were measured through the sports endurance test in the athletes, comparing the results before and after the intervention. Results: The exercise index on oblique plates for 30 seconds in the experimental group increased from 58.51±1.06 to 61.17±1.44; there was a change in the 800-meter run from 2.39±0.02 to 2.33±0.02 minutes after the experiment, and the 30kg supine weightlifting in 30 seconds was from 49.93±1.99 to 53.58±1.79. However, the control group data did not change significantly. Conclusion: Long-term appropriate high-intensity interval training played an evident role in improving the endurance of volleyball players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. RESUMO Introdução: Acredita-se que o método de treinamento intervalado de alta intensidade possa desempenhar uma melhoria da resistência esportiva nos jogadores de voleibol devido a sua característica de alternância entre períodos curtos de exercício aeróbico intenso e recuperação. Objetivo: Analisar os impactos do treinamento intervalado de alta intensidade sobre a resistência esportiva em jogadores de voleibol com idade entre 18 e 19 anos. Métodos: Foram selecionados 16 voluntários masculinos atletas de voleibol, divididos em grupo controle e experimental. Ao grupo experimental foi acrescido um protocolo de treinamento intervalado de alta intensidade, enquanto o controle permaneceu com o treinamento padrão. Os índices do efeito do treinamento intensivo intervalado foram mensurados através do teste de endurance esportiva nos atletas, comparando os resultados antes e após a intervenção. Resultados: O índice de exercício nas placas oblíquas por 30 segundos no grupo experimental aumentou de 58,51±1,06 para 61,17±1,44; houve variação na corrida de 800 metros de 2,39±0,02 para 2,33±0,02 minutos após o experimento, e o levantamento de peso supino de 30kg em 30 segundos foi de 49,93±1,99 para 53,58±1.79. Porém, os dados do grupo de controle não sofreram alterações significativas. Conclusão: O treinamento intervalado de alta intensidade apropriado à longo prazo desempenhou um papel evidente no aprimoramento sobre a endurance dos jogadores de voleibol. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. RESUMEN Introducción: Se cree que el método de entrenamiento de intervalos de alta intensidad puede desempeñar un papel en la mejora de la resistencia deportiva en jugadores de voleibol debido a su característica de alternar períodos cortos de ejercicio aeróbico intenso y recuperación. Objetivo: Analizar el impacto del entrenamiento de intervalos de alta intensidad sobre la resistencia deportiva en jugadores de voleibol, con edades comprendidas entre los 18 y 19 años. Métodos: Se seleccionaron 16 voluntarios varones deportistas de voleibol, divididos en grupo control y experimental. Al grupo experimental se le añadió un protocolo de entrenamiento de intervalos de alta intensidad, mientras que el control permaneció con el entrenamiento estándar. Los índices del efecto del entrenamiento de intervalos de alta intensidad fueron medidos a través del test de resistencia deportiva en los atletas, comparando los resultados antes y después de la intervención. Resultados: El índice de ejercicio en planchas oblicuas durante 30 segundos en el grupo experimental aumentó de 58,51±1,06 a 61,17±1,44; hubo variación en la carrera de 800 metros de 2,39±0,02 a 2,33±0,02 minutos después del experimento, y el levantamiento de pesas supino de 30 kg en 30 segundos fue de 49,93±1,99 a 53,58±1,79. Sin embargo, los datos del grupo de control no cambiaron significativamente. Conclusión: El entrenamiento de intervalos de alta intensidad adecuado a largo plazo desempeñó un papel evidente en la mejora de la resistencia de los jugadores de voleibol. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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- 2023
39. Experimental Investigation of the Secondary Changes in Imaging After Lumbar Disc Nucleoplasty in Rabbits
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Haidong Yin, Xinmei Zhang, Zhiping Huang, Xiuhua Wu, and Qingan Zhu
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Surgery ,Neurology (clinical) - Abstract
This study explored changes in imaging after lumbar disc nucleoplasty in rabbits.Twenty-four rabbits were randomly selected for X-ray, computerized tomography (CT), and magnetic resonance imaging (MRI) at 2, 6, and 12 weeks post operation. Moreover, their L3/4, L4/5, and L5/6 intervertebral discs were randomly selected as the untreated, annulus puncture, and nucleoplasty groups, respectively. Changes in disc height index (DHI%) and MRI grade were measured and compared. CT three-dimensional reconstruction was used to evaluate adjacent bone endplate changes.The untreated group's DHI% decreased slightly at different time points (p 0.05), while that of the nucleoplasty and annulus puncture groups decreased progressively (p 0.05). At six weeks post operation, the nucleoplasty group's DHI% was significantly lower than that of the annulus puncture group (p 0.05), with mild osteosclerosis and local rough changes in the endplate. At 12 weeks post operation, a "bone bridge" connection was observed in the nucleoplasty group. There was no significant difference in MRI grade between the untreated and annulus puncture groups at different time points (p 0.05). MRI grades of the intervertebral disc in the nucleoplasty and annulus puncture groups showed a progressive increase (p 0.05). Compared with the annulus puncture at the same time point, the nucleoplasty group's MRI grade of the intervertebral disc was significantly higher (p 0.05). Thus, damage caused by an annulus puncture can lead to progressive degeneration of the lumbar disc.Nucleoplasty may have a cumulative effect with the injury of the annulus puncture. Clinicians need to comprehensively consider advantages and disadvantages of nucleoplasty, strictly grasp indications of treatment, and prevent long-term complications.
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- 2022
40. Fuzzy Control on Voltage/Reactive Power in Electric Power Substation.
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Xiuhua Wu, Jun-cheng Wang, Ping Yang, and Zai-lin Piao
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- 2009
- Full Text
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41. Modeling and Designing of A Nonlineartemperature-Humidity Controller Using Inmushroom-Drying Machine.
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Xiuhua Wu, Haiyan Luo, and Minhui Shi
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- 2008
- Full Text
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42. Unilateral cervical spinal cord injury induces bone loss and metabolic changes in non-human primates (Macaca fascicularis)
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Zhou Yang, Qingan Zhu, Qi Liu, Junhao Liu, Jianyang Ding, Jie Liu, Hui Jiang, Zucheng Huang, Xiaoliang Wu, Rong Li, Xiaolin Xu, Zhiping Huang, and Xiuhua Wu
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Pathology ,medicine.medical_specialty ,business.industry ,Osteoporosis ,Bone microarchitecture ,Non-human primates (NHP) ,Diseases of the musculoskeletal system ,Spinal cord injury ,medicine.disease ,Spinal cord ,Muscle atrophy ,Bone remodeling ,medicine.anatomical_structure ,RC925-935 ,N-terminal telopeptide ,Medicine ,Orthopedics and Sports Medicine ,Cortical bone ,Original Article ,medicine.symptom ,business ,Cancellous bone - Abstract
Background/Objective The deleterious effects of chronic spinal cord injury (SCI) on the skeleton in rats, especially the lower extremities, has been proved previously. However, the long-term skeletal changes after SCI in non-human primates (NHP) have been scarcely studied. This study aimed to evaluate the bone loss in limbs and vertebrae and the bone metabolic changes in NHP after unilateral cervical spinal cord contusion injury. Methods Twelve Macaca fascicularis were randomly divided into the SCI (n=8) and the Sham (n=4) groups. The SCI models were established using hemi-contusion cervical spinal cord injury on fifth cervical vertebra (C5), and were further evaluated by histological staining and neurophysiological monitoring. Changes of bone microstructures, bone biomechanics, and bone metabolism markers were assessed by micro-CT, micro-FEA and serological kit. Results The NHP hemi-contusion cervical SCI model led to consistent unilateral limb dysfunction and potential plasticity in the face of loss of spinal cord. Furthermore, the cancellous bone mass of ipsilateral humerus and radius decreased significantly compared to the contralateral side. The bone volume fraction of humerus and radius were 17.2% and 20.1% on the ipsilateral while 29.0% and 30.1% on the contralateral respectively. Similarly, the thickness of the cortical bone in the ipsilateral forelimbs was significantly decreased, as well as the bone strength of the ipsilateral forelimbs. These changes were accompanied by diminished concentration of osteocalcin and total procollagen type 1 N-terminal propeptide (t-P1NP) as well as increased level of β-C-terminal cross-linking telopeptide of type 1collagen (β-CTX) in serological testing. Conclusions The present study demonstrated that hemi-SCI induced loss of bone mass and compromised biomechanical performance in ipsilateral forelimbs, which could be indicated by both muscle atrophy and serological changes of bone metabolism, and associated with a consistent loss of large-diameter cells of sensory neurons in the dorsal root ganglia. The Translational potential of this Article Our study, for the first time, demonstrated the bone loss in limbs and vertebrae as well as the bone metabolic changes in non-human primates after unilateral spinal cord injury (SCI). This may help to elucidate the role of muscle atrophy, serological changes and loss of sensory neurons in the mechanisms of SCI-induced osteoporosis, which would be definitely better compared with rodent models.
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- 2021
43. The Hardware Research of Dual-port RAM for Main-spare CPU in Rural Power Terminal System of Power Quantity Collection.
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Ping Yang, Shu Dai, Xiuhua Wu, and Yong Yang
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- 2007
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44. Comparison of the anatomical morphology of cervical vertebrae between humans and macaques: related to a spinal cord injury model
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Zucheng Huang, Hui Jiang, Qi Liu, Zhou Yang, Qingan Zhu, Zhiping Huang, Xushi Chen, Xiuhua Wu, and Junhao Liu
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Male ,0301 basic medicine ,Original ,non-human primate ,cervical spine ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Cerebrospinal fluid ,spinal cord injury model ,Animals ,Humans ,Medicine ,Spinal canal ,Spinal cord injury ,Spinal Cord Injuries ,General Veterinary ,medicine.diagnostic_test ,anatomical parameters ,business.industry ,Magnetic resonance imaging ,X-Ray Microtomography ,General Medicine ,Anatomy ,Spinal cord ,medicine.disease ,Magnetic Resonance Imaging ,Cervical spine ,Disease Models, Animal ,Macaca fascicularis ,030104 developmental biology ,medicine.anatomical_structure ,Spinal Cord ,Cervical spinal cord injury ,Cervical Vertebrae ,Animal Science and Zoology ,business ,Spinal Canal ,030217 neurology & neurosurgery ,Cervical vertebrae - Abstract
Non-human primates are most suitable for generating cervical experimental models, and it is necessary to study the anatomy of the cervical spine in non-human primates when generating the models. The purpose of this study was to provide the anatomical parameters of the cervical spine and spinal cord in long-tailed macaques (Macaca fascicularis) as a basis for cervical spine-related experimental studies. Cervical spine specimens from 8 male adult subjects were scanned by micro-computed tomography, and an additional 10 live male subjects were scanned by magnetic resonance imaging. The measurements and parameters from them were compared to those of 12 male adult human subjects. Additionally, 10 live male subjects were scanned by magnetic resonance imaging, and the width and depth of the spinal cord and spinal canal and the thickness of the anterior and posterior cerebrospinal fluid were measured and compared to the relevant parameters of 10 male adult human subjects. The tendency of cervical parameters to change with segmental changes was similar between species. The vertebral body, spinal canal, and spinal cord were significantly flatter in the human subjects than in the long-tailed macaques. The cerebrospinal fluid space in the long-tailed macaques was smaller than that in the human subjects. The anatomical features of the cervical vertebrae of long-tailed macaques provide a reference for establishing a preclinical model of cervical spinal cord injury.
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- 2021
45. Circular RNA FAT atypical cadherin 1 (circFAT1)/microRNA-525-5p/spindle and kinetochore-associated complex subunit 1 (SKA1) axis regulates oxaliplatin resistance in breast cancer by activating the notch and Wnt signaling pathway
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Ye Yao, Xiaoqin Li, Bobo Wu, Lihua Cheng, and Xiuhua Wu
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Chromosomal Proteins, Non-Histone ,circfat1 ,Apoptosis ,Breast Neoplasms ,Bioengineering ,Applied Microbiology and Biotechnology ,breast cancer ,Circular RNA ,Cell Line, Tumor ,ska1 ,microRNA ,Humans ,Neoplasm Metastasis ,Wnt Signaling Pathway ,Gene knockdown ,Messenger RNA ,Base Sequence ,Receptors, Notch ,Cadherin ,Chemistry ,oxaliplatin ,Wnt signaling pathway ,RNA, Circular ,mir-525-5p ,General Medicine ,Middle Aged ,Up-Regulation ,Cell biology ,Blot ,MicroRNAs ,Drug Resistance, Neoplasm ,Gene Knockdown Techniques ,Female ,TP248.13-248.65 ,Research Article ,Research Paper ,Biotechnology - Abstract
Increasing evidence has confirmed the vital roles of circular RNAs (CircRNAs) in the drug resistance of breast cancer (BC). Herein, we intended to study the effect of circular RNA FAT atypical cadherin 1 (circFAT1) on BC oxaliplatin (OX) resistance and find out the potential molecular mechanism in it. In this study, mRNA and protein levels of genes were measured by RT-qPCR and western blotting, respectively. Luciferase reporter assay confirmed the relationship between microRNA-525-5p (miR-525-5p) and circFAT1 or spindle and kinetochore-associated complex subunit 1 (SKA1). CCK-8, transwell, and flow cytometry experiments were utilized to investigate the chemosensitivity, migration, invasion, and apoptosis of BC cells. Gene Set Enrichment Analysis (GSEA) was applied to discover possible pathways related to SKA1. It was uncovered that circFAT1 was overexpressed in OX-resistant BC tissues and cells. Functional experiments showed that circFAT1 depletion reduced the level of chemoresistance-related genes. Moreover, circFAT1 knockdown remarkably facilitated apoptosis and decreased OX (half-maximal inhibitory concentration) IC50 value, migration, and invasion in OX-resistant BC cells. It was identified that miR-525-5p directly targeted circFAT1 and SKA1. Besides, rescue assays exhibited that circFAT1 promoted OX resistance in BC cells via the miR-525-5p/SKA1 regulatory network. Furthermore, GSEA and western blotting identified that SKA1 activated the Notch and Wnt pathway in OX-resistant BC cells. In conclusion, our results demonstrated that circFAT1 conferred OX resistance in BC by regulating the miR-525-5p/SKA1 via the Notch and Wnt pathway, providing a potential therapeutic target for patients with OX-resistant BC., Graphical Abstract
- Published
- 2021
46. Ketone Metabolite β-Hydroxybutyrate Ameliorates Inflammation After Spinal Cord Injury by Inhibiting the NLRP3 Inflammasome
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Junhao Liu, Ganggang Kong, Zhiping Huang, Junyu Lin, Zhou Yang, Qingan Zhu, Rong Li, Xiuhua Wu, Xiaoliang Wu, and Zucheng Huang
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0301 basic medicine ,Microglia ,medicine.medical_treatment ,Metabolite ,Inflammation ,Inflammasome ,General Medicine ,Pharmacology ,Biochemistry ,Proinflammatory cytokine ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,chemistry ,medicine ,medicine.symptom ,Receptor ,030217 neurology & neurosurgery ,Neuroinflammation ,Ketogenic diet ,medicine.drug - Abstract
Ketogenic diet (KD) has been shown to be beneficial in a range of neurological disorders, with ketone metabolite β-hydroxybutyrate (βOHB) reported to block the nucleotide oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome in bone marrow-derived macrophages. In this study, we show that pretreatment with KD or in situ βOHB suppressed macrophages/microglia activation and the overproduction of inflammatory cytokines, while KD downregulated the expression of NLRP3 inflammasome. Moreover, KD promoted macrophages/microglia transformation from the M1 phenotype to the M2a phenotype following spinal cord injury (SCI) in the in vivo study. Rats in the KD group demonstrated improved behavioral and electrophysiological recovery after SCI when compared to those rats in the standard diet group. The in vitro study performed on BV2 cells indicated that βOHB inhibited an LPS+ATP-induced inflammatory response and decreased NLRP3 protein levels. Our data demonstrated that pretreatment with KD attenuated neuroinflammation following SCI, probably by inhibiting NLRP3 inflammasome and shifting the activation state of macrophages/microglia from the M1 to the M2a phenotype. Therefore, the ketone metabolite βOHB might provide a potential future therapeutic strategy for SCI.
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- 2020
47. A Cervical Spinal Cord Hemi-Contusion Injury Model Based on Displacement Control in Non-Human Primates (Macaca fascicularis)
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Qi Liu, Xiaoliang Wu, Junyu Lin, Zhou Yang, Yuefeng Li, Qingan Zhu, Joey Fu, Wolfram Tetzlaff, Jie Liu, John L.K. Kramer, Rong Li, Jianting Chen, Zhiping Huang, Ruoyao Li, Zucheng Huang, Yongquan Cheng, Hui Jiang, Matt S. Ramer, Ganggang Kong, Junhao Liu, Brian K. Kwon, Yong Hu, Yapu Liu, and Xiuhua Wu
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030506 rehabilitation ,biology ,business.industry ,food and beverages ,medicine.disease ,Spinal cord ,humanities ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Displacement control ,biology.animal ,medicine ,Primate ,Neurology (clinical) ,Injury model ,0305 other medical science ,business ,Spinal cord injury ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Non-human primate (NHP) spinal cord injury (SCI) models can be informative in the evaluation of treatments that show promise in rodent models prior to translation to humans. In the present study, w...
- Published
- 2020
48. Clinical manifestations of Behçet’s disease in a large cohort of Chinese patients: gender- and age-related differences
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Lu Li, Chaoran Li, Xiaofeng Zeng, Xiuhua Wu, Jiaxin Zhou, Li Wang, Xinping Tian, Jinjing Liu, Wenjie Zheng, and Jing Shi
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030203 arthritis & rheumatology ,medicine.medical_specialty ,business.industry ,General Medicine ,Disease ,Behcet's disease ,medicine.disease ,Rheumatology ,Large cohort ,Age and gender ,03 medical and health sciences ,0302 clinical medicine ,Interquartile range ,Internal medicine ,Medicine ,Sex organ ,030212 general & internal medicine ,Positive pathergy test ,business - Abstract
To investigate the clinical characteristics of Bechet’s disease (BD) with respect to gender and age in China. Clinical data of 489 BD patients admitted to Peking Union Medical College Hospital (PUMCH) and registered by the BD registry database established by PUMCH from March 2014 to January 2019 were collected and analyzed in this study. Demographics, medical histories, clinical manifestations, laboratory test results, imaging findings, assessment of disease activity, treatment regimen, as well as the outcomes were reviewed. A total of 489 BD patients were included in this study, among them 286 males (58.5%) and 203 females (41.5%) with a male-to-female ratio of 1.41 and a median age of 34 years (interquartile range 28–44 years old). The results revealed that ocular lesions (P = 0.005), vascular lesions(P
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- 2020
49. Dependability Argument Metamodeling of Networked Software
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Shuanqi Wang, Xiuhua Wu, Hang Yu, Zhibo Li, and Wei Wu
- Published
- 2022
50. Combination of Colour Duplex and Contrast Enhanced Ultrasound as an Alternative to Computed Tomography Angiography in Isolated Mesenteric Artery Dissection Surveillance
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Lihua Cheng, Xiuhua Wu, Zhongzhi Jia, Feng Tian, Kai Wang, and Bobo Wu
- Subjects
Adult ,Male ,Computed Tomography Angiography ,False lumen ,Contrast Media ,030204 cardiovascular system & hematology ,030230 surgery ,Conservative Treatment ,Multimodal Imaging ,03 medical and health sciences ,0302 clinical medicine ,Aneurysm ,medicine ,Humans ,In patient ,cardiovascular diseases ,Artery dissection ,Aged ,Computed tomography angiography ,Ultrasonography, Doppler, Duplex ,medicine.diagnostic_test ,business.industry ,musculoskeletal, neural, and ocular physiology ,Endovascular Procedures ,Ultrasound ,Middle Aged ,medicine.disease ,Mesenteric Arteries ,Aortic Dissection ,Duplex (building) ,Feasibility Studies ,Female ,Stents ,Surgery ,Cardiology and Cardiovascular Medicine ,business ,Nuclear medicine ,psychological phenomena and processes ,Follow-Up Studies ,Contrast-enhanced ultrasound - Abstract
The aim was to investigate the effectiveness of colour duplex ultrasound (CDU) plus contrast enhanced ultrasound (CEUS) vs. computed tomography angiography (CTA) for surveillance in patients with isolated mesenteric artery dissection (IMAD).Patients who underwent CDU, CEUS, and CTA for surveillance of IMAD between January 2012 and May 2019 were included in the study. The accuracy of CDU, CEUS, and CTA for determining the morphological characteristics of IMAD was analysed.A total of 42 patients undergoing 76 total imaging examinations during follow up were included. Both CTA and CDU plus CEUS demonstrated the thrombosed false lumen for 28 (36.8%) examinations and the dissecting aneurysm for 20 (26.3%) examinations (both κ = 1.0). The diameter of the dissecting aneurysm was 5.03 ± 1.25 mm using CDU and CEUS vs. 5.27 ± 1.23 mm on CTA (coefficient of consistency, 0.997; p .001). The entry points were visualised by CDU and CEUS for 20 (26.3%) examinations and by CTA for 14 (18.4%) examinations (κ = 0.769); no re-entry points were visualised by CDU and CEUS for any examinations but re-entry points were visualised by CTA for two (2.6%) examinations. The minimum inner diameter was 2.80 ± 1.30 mm on CDU and CEUS vs. 2.52 ± 1.29 mm on CTA (coefficient of consistency, 0.999; p .001). The peak systolic velocities were 128.2 ± 13.0 cm/s at diagnosis and 98.7 ± 4.9 cm/s after one month (p .001).The combination of CDU and CEUS can be used in place of CTA for the surveillance of IMAD.
- Published
- 2019
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