1. Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway.
- Author
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Chen F, Xiong BR, Xian SY, Zhang J, Ding RW, Xu M, and Zhang ZZ
- Subjects
- Animals, Humans, Mice, Spinal Cord metabolism, Mice, Knockout, Male, Female, Adult, Middle Aged, AMP-Activated Protein Kinases metabolism, Fibroblast Growth Factor 5 genetics, Fibroblast Growth Factor 5 metabolism, Oxidative Stress, Spinal Cord Injuries metabolism
- Abstract
Excessive productions of inflammatory cytokines and free radicals are involved in spinal cord injury (SCI). Fibroblast growth factor 5 (FGF5) is associated with inflammatory response and oxidative damage, and we herein intend to determine its function in SCI. Lentivirus was instilled to overexpress or knockdown FGF5 expression in mice. Compound C or H89 2HCl were used to suppress AMP-activated protein kinase (AMPK) or protein kinase A (PKA), respectively. FGF5 level was significantly decreased during SCI. FGF5 overexpression mitigated, while FGF5 silence further facilitated inflammatory response, oxidative damage and SCI. Mechanically, FGF5 activated AMPK to attenuate SCI in a cAMP/PKA-dependent manner, while inhibiting AMPK or PKA with pharmacological methods significantly abolished the neuroprotective effects of FGF5 against SCI. More importantly, serum FGF5 level was decreased in SCI patients, and elevated serum FGF5 level often indicate better prognosis. Our study identifies FGF5 as an effective therapeutic and prognostic target for SCI., (© 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
- Published
- 2023
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