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FOXD2 Adjacent Opposite Strand RNA 1 (FOXD2-AS1), a long noncoding RNA (lncRNA), exhibits specifically elevated in numerous cancerous cells. Numerous studies have shown that FOXD2-AS1 encourages cellular proliferation, migration and invasion. Nevertheless, the exact mechanism through which FOXD2-AS1 contributes to prostate cancer (PCa) remains unclear. Consequently, we aimed to explore the implications of FOXD2-AS1 on the growth of PCa. Initially, an elevation of FOXD2-AS1 observed in PCa cells (PC-3, DU145 and Lncap) than the prostate normal cell line RWPE2. Then, PC-3 cells were tranafected with shFOXD2-AS1, sh-Numerical Control (shNC) or FOXD2-AS1 to assess the implications of FOXD2-AS. Cell growth was measured with cell counting kit-8 (CCK8) and 5-ethynyl-2′-deoxyuridine (EDU) assays, and cell invasion and migration were assessed by Transwell assays, which demonstrated that FOXD2-AS1 silence impeded proliferation, migration and invasion of PC-3 cells. Additionally, we discovered that FOXD2-AS1 bonded with miR-206/programmed cell death protein 10 (PDCD10) trough analyzing the interaction sites of lncRNA, miRNA and protein. Then, these interaction abilities were confirmed by dual-luciferase reporter assays and RT-qPCR, suggesting FOXD2-AS1 could upregulate the amount of PDCD10 through suppressing miR-206. Furthermore, the role of FOXD2-AS1 silencing on PCa carcinogenesis were assessed. In vivo experiment, shFOXD2-AS1 led to a notable reduction in both the size and weight of PCa. These findings indicated that FOXD2-AS1 silencing effectively hindered the progression of prostate cancer. In conclusion, the upregulation of FOXD2-AS1 was observed in PCa, and the knockdown of FOXD2-AS1 could alleviated tumor development by targeting miR-206 to upregulate PDCD10 expression.

Abstract Background BAT1706 is a proposed biosimilar of bevacizumab, a vascular endothelial growth factor A (VEGF-A)-targeting biologic used to treat several different cancers, including metastatic colorectal cancer. A comprehensive physicochemical and functional similarity assessment is a key component of demonstrating biosimilarity between a reference biologic and a proposed biosimilar. Here we report the physicochemical and functional similarity of BAT1706 and reference bevacizumab sourced from both the United States (US-bevacizumab) and the European Union (EU-bevacizumab). Method A large range of product attributes, including primary and higher order structure, post-translational modifications, purity, stability, and potency, were characterized for BAT1706 and EU/US-bevacizumab using sensitive state-of-the-art analytical techniques. Up to 18 lots of US- and 29 lots of EU-bevacizumab, and 10 unique drug substance lots of BAT1706, were assessed. Result BAT1706 was shown to have an identical amino acid sequence and an indistinguishable higher-order structure compared with EU/US-bevacizumab. BAT1706 and EU/US-bevacizumab also exhibited similar post-translational modifications, glycan profiles, and charge variants. Potency, assessed using a wide range of bioassays, was also shown to be comparable between BAT1706 and EU/US-bevacizumab, with statistical equivalence demonstrated for VEGF-A binding and neutralizing activity. Conclusion Overall, this extensive comparability exercise demonstrated BAT1706 to match EU/US-bevacizumab in terms of all physicochemical and functional attributes assessed.

ObjectiveTo explore the effectiveness of mindfulness yoga training on sleep quality in college students， so as to provide references for the intervention of sleep problems in college students.MethodsFrom March 28， 2021 to April 20， 2021， A sample of 45 college students with Pittsburgh Sleep Quality Index （PSQI） score ≥8 was recruited. Participants were randomly divided into study group （n=22） and control group （n=23）. Participants in the intervention group received mindfulness yoga training twice a week for 4 weeks. The control group did not receive mindfulness yoga training during the trial， but the same training would be given after the trial. Before and after the intervention， PSQI and the N-back training tasks were used to evaluate the sleep quality and working memory ability of the two groups.ResultsThe main effects of time on the PSQI total score， the accuracy and reaction time of N-back training task were statistically significant （F=37.240， 6.898， 10.775， P

OX40 (CD134), a member of the TNF receptor superfamily, is a widely studied costimulatory immune checkpoint. Several OX40 agonistic antibodies are in the clinical stage for cancer treatment, among which PF-04518600 is the leader and currently in phase II trial. It has been recognized that one potential mode of action for anti-OX40 antibodies is the deletion of intratumoral Tregs. Thus, a novel human anti-OX40 antibody, BAT6026, was generated with enhanced antibody dependent cellular cytotoxicity (ADCC) via fucose deletion to strengthen its Treg depletion activity. This characteristic of BAT6026 differentiates it from other previously reported anti-OX40 antibodies in the field of tumor therapy. The affinity of BT6026 to OX40 was 0.28nM, approximately 8 times stronger than that of PF-04518600. BAT6026 effectively competed for the binding of ligand OX40L to OX40, whereas PF-04518600 only partially competed. Moreover, compared to PF-04518600, BAT6026 activated T cells more effectively when clustered by FcγRs engagement and stimulated SEB-pretreated PBMCs to secrete IL-2 cytokines in vitro. In addition, BAT6026 demonstrated stronger anti-tumor activity than PF-04518600 in an OX40-humanized mouse MC38 tumor model. BAT6026 also showed a significantly synergistic effect on tumor inhibition when combined treatment with PD-1 antibody. Analysis of tumor-infiltrating T cells revealed that BAT6026 treatment significantly reduced Treg cells and increased CD8+ T cells in tumor. Preclinical safety assessment in non-human primates demonstrated a good safety profile for BAT6026. Together these data warrant further development of BAT6026 into clinical trials for patients with cancer.

The efficient global optimization (EGO) algorithm is a kind of Bayesian optimization algorithm that uses the Kriging interpolation model and expectation improvement (EI) criteria as surrogate model and acquisition function, respectively. However, the greediness of EI criteria can lead the EGO algorithm to fall into local optima. Owing to this, revised expectation improvement (REI) criteria are proposed by introducing a balance factor to adjust the exploitation and exploration of EI criteria, and the corresponding algorithm is called the revised efficient global optimization (REGO) algorithm. In order to motivate exploration, and ensure that the computational cost is acceptable, a Latin hypercube based indicator is proposed to denote a balance factor from the viewpoint of sample distribution. Several test functions and an airfoil optimization problem are applied to verify the performance of the REGO algorithm. The results show that the REGO algorithm has acceptable computational cost, a strong ability to find global optima, and good robustness. [ABSTRACT FROM AUTHOR]

Objective To investigate the protective effect of troxerutin against spinal cord injury (SCI) in rats and explore the underlying mechanisms. Methods Sixty 8-week-old male SD rats were randomly divided into sham operation group, SCI group and troxerutin treatment group. In the sham group, the rats were subjected to laminectomy without SCI; rat models of SCI were established in SCI group and troxerutin treatment group, and in the latter group, the rats received daily intravenous injection of troxerutin after SCI for 4 consecutive weeks. Neurological recovery of the rats was assessed based on the Basso-Beattie-Bresnehan (BBB) score, and HE staining was used to observe the changes in the vacuoles and blood vessels at the injury site of the spinal cord. RNA sequencing was used to examine the differential gene expressions in the injured spinal cord tissue between SCI group and troxerutin group, and Western blotting was performed to verify the differential expression of caspase-6 protein. Results Two weeks of troxerutin treatment significantly increased the BBB score of the rats as compared with the rats in SCI group (P < 0.05), though the BBB scores in both groups were still lower than that in the sham operation group. At 4 weeks of treatment, the rats in troxerutin group showed decreased vacuoles with increased angiogenesis in the injured spinal cord tissues in comparison with the rats in SCI group. RNA sequencing showed that 261 genes were differentially expressed in the injured spinal cord tissue between troxerutin group and SCI group, including 217 up-regulated and 44 down-regulated genes. GO and KEGG enrichment analysis suggested that troxerutin was capable of activating several signaling pathways including the Wnt, Hippo, TGF-β and Apoptosis pathways, which were functionally related to apoptosis, angiogenesis, neuronal formation, and vasculature. The expression of caspase-6 protein in the semi-dark zone of the injured spinal cord tissues was significantly higher in SCI group than in troxerutin group (P < 0.05). Conclusion Troxerutin shows obvious protective effect against spinal cord injury in rats, possibly by inhibiting cell apoptosis and promoting angiogenesis in the injured spinal cord.

The sugarcane top is one of the main by-products of sugarcane, which is rich in resources and comprehensive in nutritional value, and is one of the sources of roughage for ruminants. Silage is an effective method to preserve and develop sugarcane top feed, but the high content of carbohydrate and yeast makes natural silage easy to deteriorate, and the high content of fiber will affect the digestibility and palatability of the feeding animals. At present, the research on sugarcane top silage mainly focuses on the use of various additives, mixed silage with other materials, the effect of sugarcane top silage on ruminants, and the influence of sugarcane harvest time, varieties and silage time on silage. In this paper, the effects of additives, mixed silage and other factors on the quality, nutritional value, aerobic stability and feeding effect of sugarcane top silage were summarized, which provided reference for promoting the utilization of sugarcane top and the preparation of high-quality silage. [ABSTRACT FROM AUTHOR]