1. Sequence-encoded bioactive protein-multiblock polymer conjugates via quantitative one-pot iterative living polymerization
- Author
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Ziying Li, Kaiyuan Song, Yu Chen, Qijing Huang, Lujia You, Li Yu, Baiyang Chen, Zihang Yuan, Yaqin Xu, Yue Su, Lintai Da, Xinyuan Zhu, and Ruijiao Dong
- Subjects
Science - Abstract
Abstract Protein therapeutics are essential in treating various diseases, but their inherent biological instability and short circulatory half-lives in vivo pose challenges. Herein, a quantitative one-pot iterative living polymerization technique is reported towards precision control over the molecular structure and monomer sequence of protein-polymer conjugates, aiming to maximize physicochemical properties and biological functions of proteins. Using this quantitative one-pot iterative living polymerization technique, we successfully develop a series of sequence-controlled protein-multiblock polymer conjugates, enhancing their biostability, pharmacokinetics, cellular uptake, and in vivo biodistribution. All-atom molecular dynamics simulations are performed to disclose the definite sequence-function relationship of the bioconjugates, further demonstrating their sequence-encoded cellular uptake behavior and in vivo biodistribution in mice. Overall, this work provides a robust approach for creating precision protein-polymer conjugates with defined sequences and advanced functions as a promising candidate in disease treatment.
- Published
- 2024
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