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Esophageal cancer is a common malignant disease that is generally treated with radiotherapy. High mobility group box 1 (HMGB1) plays an essential role in tumor cell proliferation, migration, and cell cycle progression. Here, we aimed to clarify the effects of HMGB1 on radioresistance in esophageal squamous cell carcinoma (ESCC) cell lines and patient survival. We performed immunohistochemistry for HMGB1 in biopsy samples of 39 stage I-III ESCC patients grouped by HMGB1 expression status. Then, 1-, 3-, 5-, and 10-year overall survival outcomes were calculated by Kaplan-Meier survival analysis. The cellular localization of HMGB1 was examined before and after irradiation by Immunofluorescence staining. Stable cell lines (KYSE30 and KYSE510) with differential HMGB1 expression were constructed using lentiviruses. Furthermore, we examined phosphorylated histone H2AX (γ-H2AX) expression in both HMGB1 overexpression and negative control groups by western blotting. HMGB1-negative expression was associated with superior ESCC patient 10-year survival (

The purpose of this study was to evaluate several preradiotherapy serum inflammatory indicators, including the neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), systemic immune-inflammation index (SII), and systemic inflammation score (SIS), and compare which of these indicators had the highest value in predicting survival. Inflammatory markers were combined with traditional prognostic factors, and novel nomogram models were developed to predict overall survival (OS) and progression-free survival (PFS) for patients with esophageal squamous cell carcinoma. A total of 245 patients were enrolled. The Kaplan-Meier method and univariate and multivariate analyses were used to compare survival differences. A total of 239 patients met the eligibility criteria. The survival numbers at 1, 3, and 5 years were 176, 83, and 62, respectively. The OS and PFS rates estimated at 1, 3, and 5 years were 74.6%, 36.8%, and 26.5% and 58.4%, 31.3%, and 20.5%, respectively. The differences in patients' OS and PFS were significant when univariate analysis was applied based on inflammation-based measures. Multivariate analysis showed that tumor length, tumor stage, tumor/node/metastasis stage, chemotherapy, and SIS value were predictive variables for OS and PFS. The nomogram model established based on the multivariate models of the training data set had good predictive ability. The unadjusted C-index was 0.701 (95% CI, 0.662-0.740) and 0.695 (95% CI, 0.656-0.734) for OS and PFS, respectively. This study showed that the SIS-based nomogram could accurately predict the OS and PFS of patients with esophageal squamous cell carcinoma.

A central principle of synaptic transmission is that action potential-induced presynaptic neurotransmitter release occurs exclusively via Ca

Large dense-core vesicles (LDCVs) are larger in volume than synaptic vesicles, and are filled with multiple neuropeptides, hormones, and neurotransmitters that participate in various physiological processes. However, little is known about the mechanism determining the size of LDCVs. Here, it is reported that secretogranin II (SgII), a vesicle matrix protein, contributes to LDCV size regulation through its liquid-liquid phase separation in neuroendocrine cells. First, SgII undergoes pH-dependent polymerization and the polymerized SgII forms phase droplets with Ca

An intelligent decision analytic framework for dealing with complex decision-making risk system is presented and Bayesian network (BN) approach is utilized to evaluate the influence of multilevel uncertainty in various risks (e.g., social, natural, economic, intracompany risks) on decision-making deviation of Chinese hydropower corporations. The technique of fuzzy probability is approached to calculate intricate parameters to the question of inference learning through the sensitivity and influence power analysis, the results of back inference show that there exists the risk transformation mechanism from external uncertain risks (e.g., social risks, ecological environment factors) to hydropower corporations’ internal uncertainties closely relating to economic uncertainties through strategic planning. The study concerning identification and intelligent analysis of uncertain risks in decision-making process illustrates the feasibility and validity of applying BN and its pragmatic implications on hydropower corporations strategic planning and guidance in operational management.

In a significant period of Chinese energy reformation to the point of expediting revolution in energy production and consumption, and promoting green low-carbon upgrading transformation of energy electricity, Chinese government has to implement the obligatory policy of renewable portfolio standards (RPS) with specific institutional provisions sternly. The renewable energy quotas in thermal power industry with carbon emission abatement constraints particularly have a latent impact on the behavior of thermal electricity producers, which is ineluctably involved in electricity connection of grid companies. To make clear the positive role in boosting investment in renewable energy generation in thermal power industry under mandatory quotas requirements, we will utilize evolutionary game based on system dynamics (SD) to tackle with the sophisticated nexus among the government, thermal power producers and grid companies. We begin analysis of general evolutionary strategy stability in scenario ll by dynamically adjusting values of external variables of SD model to uncover pivotal variables affecting evolutionary strategy stability. Then in scenario I, the dynamic punishment structure consisting of cost elements to spark off the emulation of optimal manoeuvre selections of tripartite game agents is amended based on the simulation of vital variables affecting evolutionary strategy stability in scenario II. The significant conclusions provide decision-making support and management enlightenment for Chinese government to edge renewable energy generation capacity of thermal power producers and constrained degree of dynamic penalty for grid companies.

Background Recent evidence of clinical trials highlights that the combination of two noncompetitive anti-EGFR antibodies can benefit patients with several cancers. Previous studies propose that a lattice complex assembled by antibodies and EGFR down-regulates surface EGFR by rapid internalization of the complex. However, there remains a paucity of evidence and understanding on the existence of a lattice complex on cell surface and its cellular processes of internalization. Methods Herein, we used three dimensions structured illumination microscopy to directly observe the actual morphology of the lattice complex formed on Hela cell membrane after noncompetitive anti-EGFR antibody combinations, and we explored the internalized mechanism of noncompetitive antibody combinations by constructing a PIP2 consumption system. Result We observed the lattice complex (length > 1 μm) on the surface of living cell after preincubation with Cetuximab and H11, but combination of Cetuximab and single domain antibody 7D12 fails to assemble the lattice, these results demonstrates the importance of symmetrical structure of conventional antibody for lattice formation. Interestingly, the lattice complex assembles along with cytoskeletal fibers, and its internalization recruits a large amount of PIP2 and triggers the rearrangement of F-actin. Conclusions The above data suggests that large-size lattice complex affects membrane fluidity and dynamic reorganization of cytoskeletal, which may be responsible for its rapid internalization. These new insight will aid in current rational combination design of anti-EGFR antibodies.

A central principle of synaptic transmission is that action potential-induced presynaptic neurotransmitter release occurs exclusively via Ca2+-dependent secretion (CDS). The discovery and mechanistic investigations of Ca2+-independent but voltage-dependent secretion (CiVDS) have demonstrated that the action potential per se is sufficient to trigger neurotransmission in the somata of primary sensory and sympathetic neurons in mammals. One key question remains, however, whether CiVDS contributes to central synaptic transmission. Here we report, in the central transmission from presynaptic (dorsal root ganglion) to postsynaptic (spinal dorsal horn) neurons, (1) excitatory postsynaptic currents (EPSCs) are mediated by glutamate transmission through both CiVDS (up to 87%) and CDS; (2) CiVDS-EPSCs are independent of extracellular and intracellular Ca2+; (3) CiVDS is >100 times faster than CDS in vesicle recycling with much less short-term depression; (4) the fusion machinery of CiVDS includes Cav2.2 (voltage sensor) and SNARE (fusion pore). Together, an essential component of activity-induced EPSCs is mediated by CiVDS in a central synapse.

BACKGROUND: The systemic inflammation response index (SIRI) and prognostic nutritional index (PNI) have been shown to be correlated with the prognosis of various solid tumors. This study sought to investigate the prognostic value of the SIRI and the PNI individually and in combination in locally advanced elderly esophageal squamous cell carcinoma (ESCC) patients treated with radical radiotherapy. METHODS: The data of 192 ESCC patients aged ≥65 years, who had been treated with definitive radiotherapy between 2013 and 2016, were retrospectively analyzed. The optimal cutoff values of SIRI and PNI were determined by receiver operating characteristic curves. Kaplan-Meier curves and Cox proportional hazards models were used to analyze the effect of the SIRI and PNI on overall survival (OS) and progression-free survival (PFS). The areas under the curve were measured to evaluate the predictive ability of the SIRI, PNI, and SIRI combined with PNI for OS. RESULTS: The optimal cutoff values of the pretreatment SIRI and PNI were 1.03 and 49.60, respectively. The univariate and multivariate analyses demonstrated that T stage (P=0.021), TNM stage (P=0.022), synchronous chemotherapy (P=0.032), the SIRI (P=0.001), and the PNI (P=0.045) were independent prognostic factors for OS and N stage (P=0.004), synchronous chemotherapy (P=0.016) and the SIRI (P=0.004) were independent prognostic factors for PFS. The AUC of the combined SIRI and PNI (0.706; 0.612–0.801) was higher than those of the SIRI (0.648; 0.540–0.756) and the PNI (0.621; 0.523–0.720). Patients in the low-SIRI and high-PNI groups, especially those in clinical stage II or who received synchronous chemotherapy (P

The impact of preoperative radiotherapy (PRT) on survival in patients with stage II and III esophageal squamous cell carcinoma (ESCC) remains controversial. The aim of this study was to explore the effect of PRT on survival of these patients. Patients with stage II and III ESCC who underwent chemotherapy ± PRT were identified and retrieved from the SEER database from 2010 to 2015. Cox regression analysis was used to identify independent prognostic factors in patients. Subgroup analysis stratified by T stage and N stage was performed. Kaplan–Meier survival analysis was performed to assess disease specific survival (DSS). A total of 1160 patients were retrieved, of whom 289 (24.9%) underwent PRT plus chemotherapy, and 871 (75.1%) did not receive PRT. In multivariate analysis, PRT plus chemotherapy was a favorable prognostic factor for patients with stage T2 (hazard ratio [HR], 0.364, 95% CI, 0.202–0.658; P

Action potential-induced vesicular exocytosis is considered exclusively Ca2+ dependent in Katz’s Ca2+ hypothesis on synaptic transmission. This long-standing concept gets an exception following the discovery of Ca2+-independent but voltage-dependent secretion (CiVDS) and its molecular mechanisms in dorsal root ganglion sensory neurons. However, whether CiVDS presents only in sensory cells remains elusive. Here, by combining multiple independent recordings, we report that [1] CiVDS robustly presents in the sympathetic nervous system, including sympathetic superior cervical ganglion neurons and slice adrenal chromaffin cells, [2] uses voltage sensors of Ca2+ channels (N-type and novel L-type), and [3] contributes to catecholamine release in both homeostatic and fight-or-flight like states; [4] CiVDS-mediated catecholamine release is faster than that of Ca2+-dependent secretion at the quantal level and [5] increases Ca2+ currents and contractility of cardiac myocytes. Together, CiVDS presents in the sympathetic nervous system with potential physiological functions, including cardiac muscle contractility.

The use of methoxy polyethylene glycol thiol (mPEG-SH), in conjunction with additives, is one of the most straightforward, versatile, and widely used methods for modifying gold nanorods (GNRs), especially for applications in the field of biomedicines. The decreased toxicity of PEGylated GNRs is critical for investigating cell-nanomaterial interactions. Most techniques suffer from low PEGylation efficiency. Several investigations of the PEGylation process have been conducted to determine its mechanism. Herein, we systematically investigated the qualitative and quantitative effects of the pH value on GNR PEGylation efficiency. The PEGylation process was applied to modify the GNRs at pH 0.3–14. PEGylation of GNRs at pH 2.3 and 9.9 resulted in better modification efficiency than under other pH conditions, achieving average PEGylation efficiencies of 95.5% and 83.6%, respectively. In both cases, the resulting GNRs could survive centrifugation after surface modification as demonstrated by measuring their longitudinal surface plasmon resonance (LSPR) UV-Vis absorption peaks. The aggregation index correlated to the PEGylation efficiency at the various pH levels. The origin of the differences observed at different pH values was also elucidated. These findings significantly expand the potential use of the aggregation index, as well as the understanding of processes used to modify gold nanoparticles.

A low-cost Co-free CrFeNi(3-x)Alx (x = 1, 0.9, 0.8, 0.7, 0.6) eutectic high entropy alloy (EHEA) system was designed, successfully prepared and characterized. With the increase of Al content, the alloy dramatically transforms from a hypoeutectic to a hypereutectic microstructure together with an increase in strength and a decrease in ductility. The CrFeNi2.2Al0.8 alloy was found to be an eutectic high entropy alloy and was composed of face centered cubic (FCC) phase and ordered body centered cubic (B2) phase. The hypereutectic CrFeNi2Al alloy shows excellent mechanical properties with an ultimate strength of 1357 MPa and a total elongation of 6.4% at the cast condition. The excellent mechanical properties of this EHEA system can be attributed to the nanolamellar microstructure and suggests a great potential application in the engineering field. We also propose a simple strategy to design Co-free EHEAs and predict more than ten potential Co-free EHEAs to guide experimental searches.

Cities in mainland China are usually classified according to geographical locations. This traditional city classification system is limited to relative fixed factors, which lives out a gap in terms of the spatial differences of municipal solid waste (MSW). Developing a more comprehensive city classification system is essential for MSW generation prediction and waste management. In this study, six economic, social and climatic indicators that affect MSW generation: population, per capita GDP (PCGDP), environmental sanitation investment (ESI), average temperature, average precipitation, and average humidity, are selected. Weights were calculated for each indicator using a combination of CRITIC weight method and Pearson correlation coefficient prior to cluster analysis. The k-means clustering algorithm was used to classify all cities into four clusters, which differed significantly in the relationships between MSW generation and influencing factors. The results of Kruskal-Wallis test also show that cities in different clusters show different distributions in terms of the indicators selected. The cross-prediction results of the model further validate the reliability of the clustering results from a quantitative perspective. By establishing a city classification system, cities with similar relationships between MSW generation and influencing factors can be placed into one cluster. The model established in one certain city cluster can be used to predict the MSW generation for cities in the same cluster that lack historical data. This may also help to formulate appropriate regional policies according to different relationships between MSW generation and influencing factors, especially for the four city clusters in the mainland China.

Background: Several inflammatory markers have been reported to be associated with clinical outcomes in patients with esophageal squamous cell carcinoma (ESCC). This study was to evaluate several pre-radiotherapy serum inflammatory indicators, including the neutrophil / lymphocytes ratio (NLR), platelet / lymphocyte (PLR), systemic immune-inflammatory index (SII), systemic inflammation score(SIS), and compare which one has the highest predicted survival value. Finally, combining inflammatory markers with traditional prognostic factors, a new Nomogram model was developed to predict overall survival (OS) and progression-free survival (PFS) for ESCC patients receiving radiotherapy (RT) or chemoradiotherapy (CRT). Methods: This study retrospectively reviewed the data of 245 patients with thoracic esophageal squamous cell carcinoma (ESCC) underwent RT or CRT in the Fourth Hospital of Hebei Medical University from January 2013 to December 2015. The survival differences of these indexes were compared by the Kaplan-Meier method, and the univariate and the multivariate analyses were performed to determine these prognostic factors of overall survival (OS) and progression-free survival (PFS). Multivariate Cox proportional hazards regression models were used to create nomogram for OS and PFS.Results: 239 patients met the eligibility criteria. The estimated 1-, 3-, and 5-year OS and PFS rates were 74.6%, 36.8%, 26.5% and 58.4%, 31.3%, 20.5%, respectively, for the whole group. The difference in survival between OS and PFS was significant when univariate analysis were applied based on these inflammation-based measures. Multivariate analysis showed that tumor length, T stage, TNM stage, chemotherapy, SIS were predictive variables for OS and PFS in the multivariate model. The nomogram model established based on multivariate models of training data set had good predictive ability, the unadjusted C-index was 0.701 (95% CI, 0.662– 0.740) and 0.695 (95% CI, 0.656 - 0.734) for OS and PFS. Conclusions: This study show that SIS, as a comprehensive indicator of inflammation and nutrition, had the strongest predictive power for evaluating prognosis. Moreover, our nomogram can accurately predict OS and PFS after treatment and may provide guidance regarding adjuvant therapy and surveillance.

Additional file 1: Table S1. Binding sites of anti-EGFR antibodies.

To achieve balance of high strength and high ductility remains a difficult problem for metallic materials, especially in as-cast alloys. We combined the design concepts of high-entropy alloys (HEAs) and Ni-based superalloys, and designed two new Ni48.7Co17.5Cr10Fe9.3Al7.5Ti7 and Ni47.7Co17.5Cr10Fe9.3Al7.5Ti7Mo1 (at%) precipitation-hardened cast HEAs. Both two alloys have face-centered cubic (fcc) high-entropy matrix + nano γ′ (Ni3(Al,Ti)-type) particles structure. Fine γ′ particles with the average diameter less than 70 nm and 130 nm are homogeneously distributed in dendritic regions and inter-dendritic regions, respectively. The yield strength of Ni47.7Co17.5Cr10Fe9.3Al7.5Ti7Mo1 alloy is 899 MPa, ultimate tensile strength is 1064 MPa and elongation is 14.3%. The strengthening theory is discussed. Attributable to substantial γ′ precipitates, shearing strengthening mechanism plays an important role. Fracture is caused by micron-sized eutectic γ′ phases that lead to stress concentration.

Eutectic high entropy alloys (EHEAs) have attracted wide attention of material scientists and engineering scientists due to the good castability, phase stability as well as excellent mechanical properties. However, it still remains a huge challenge to design EHEAs accurately on account of the inaccuracy of multicomponent phase diagram calculation and complexity of experimental exploration. Here, a simple and practicable pseudo binary method was proposed to design EHEAs using the parameters of valence electron concentration (VEC) and mixing enthalpy (△Hmix). Using this strategy, a series of nanostructured EHEAs composed of ordered body centered cubic (B2) phase and face centered cubic (FCC) phase were successfully developed. All the designed alloys in the current work exhibited excellent comprehensive mechanical properties, with a tensile fracture strength higher than 1000 MPa and a total elongation > 10%. The combination of high strength and good plasticity indicates a significant potential application in engineering field. Furthermore, this new strategy is not only suitable for the current work but also available for the majority of reported EHEAs, which shows great universality in designing EHEAs. Keywords: Eutectic high entropy alloys, Mechanical properties, Microstructure design, Mixing enthalpy, Valence electron concentration

The present work reports two novel γ′(Ni3Al type)-enhanced high-entropy superalloys Ni45 − x(FeCoCr)40(AlTi)15Hfx (x = 0, 0.2 at.%) with high-entropy fcc (face centered cubic) matrix and γ′ particles microstructure at cast condition. A large number of γ′ particles, with an average diameter size of 52 nm (dendritic regions) and 90 nm (inter-dendritic regions), are homogeneously distributed in a high-entropy fcc matrix and are coherent with the matrix. Attributable to the γ′ particles, the alloys show prominent mechanical properties at both room temperature and high temperatures. Coherent strength and atomic ordering strength are the primary strengthening mechanisms.

A non-equiatomic Fe 34 Cr 34 Ni 14 Al 14 Co 4 high-entropy alloy was designed and prepared. The alloy displayed novel nanostructures consisting of hierarchical Fe-Cr-rich and Ni-Al-rich phases. As a result, the alloy exhibited good compressive mechanical properties both at room and elevated temperatures, superior to many high-entropy alloys and conventional alloys.

High-entropy alloys have attracted much attention due to their unique microstructures and excellent properties. Since their invention more than ten years ago, research attention has been mainly focused on the study of multicomponent alloys with equiatomic or near-equiatomic compositions. Here we propose a novel design of non-equiatomic medium-entropy alloys that contain one matrix element and several equiatomic alloying elements. To verify the utility of this new design, a series of Co-free Fex(CrNiAl)100−x (at.%, 25 ≤ x ≤ 65) medium-entropy alloys were designed from the much-studied FeNiCrCoAl high-entropy alloy. Detailed characterization reveals that the alloys exhibit novel two-phase microstructures consisting of B2-ordered nanoprecipitates and BCC-disordered matrix. As the alloys deviate far from equiatomic composition, the structure of the nanoprecipitates transfers from a spinodal-like intertwined structure to a nanoparticle dispersed structure. Previous parametric approaches to predict phase formation rules for high-entropy alloys are unable to describe the phase separation behaviors in the studied alloys. Our findings provide a new route to design medium-entropy alloys and also demonstrate a strategy for designing nanostructured alloys from multicomponent alloy systems through simple variations in non-equiatomic compositions.

This study demonstrated the fabrication of the gold nanorods/zinc oxide photodetector (GNRs/ZnO PD) and PEGylated GNRs were decorated onto the planar ZnO device via the microchannel relocation process. Several properties, including the sensitivity, responsibility, external quantum efficiency (EQE) and on/off switching ratio, were characterised under the 365 nm of the UV irradiation. The GNRs/ZnO PD performed stable and reproducible photocurrent responses and a high on/off switching ratio under the UV irradiation cycles. The photocurrent and Ilight/Idark current ratio increased to approximately 9 times and 167, respectively. Furthermore, the UV responsibility was enhanced to 180 A/W and the EQE was also improved to 612. These performance enhancements are dominated by the localised surface plasmon resonance (LSPR) effect of decorated GNRs. PEGylated GNRs have longitudinal and transverse LSPR peaks around 510 nm and 696 nm, respectively. The LSPR effect of decorated GNRs assists to enhance the UV emission of the ZnO PD by 25 times.

Eutectic high entropy alloys (EHEAs) are recently of great interest due to their excellent mechanical properties. However, only a few EHEAs have so far been designed because of the lack of multi-component phase diagrams. Here, a new Fe20Co20Ni41Al19 EHEA was successfully designed and studied. The alloy exhibited a nano-lamellar eutectic microstructure composed of ordered BCC (B2) phase and ordered FCC (L12) phase. The room-temperature tensile strength and total elongation of the alloy were as high as 1103 MPa and 18.7%, respectively, which were better than those of a majority of as-cast high entropy alloys. The new alloy has a potential application in industry and will promote the further study of EHEAs.

Action potential-induced vesicular exocytosis is considered exclusively Ca(2+) dependent in Katz’s Ca(2+) hypothesis on synaptic transmission. This long-standing concept gets an exception following the discovery of Ca(2+)-independent but voltage-dependent secretion (CiVDS) and its molecular mechanisms in dorsal root ganglion sensory neurons. However, whether CiVDS presents only in sensory cells remains elusive. Here, by combining multiple independent recordings, we report that [1] CiVDS robustly presents in the sympathetic nervous system, including sympathetic superior cervical ganglion neurons and slice adrenal chromaffin cells, [2] uses voltage sensors of Ca(2+) channels (N-type and novel L-type), and [3] contributes to catecholamine release in both homeostatic and fight-or-flight like states; [4] CiVDS-mediated catecholamine release is faster than that of Ca(2+)-dependent secretion at the quantal level and [5] increases Ca(2+) currents and contractility of cardiac myocytes. Together, CiVDS presents in the sympathetic nervous system with potential physiological functions, including cardiac muscle contractility.

The brain’s small-world network utilizes its short-range and long-range synaptic connections to process information in a complex and energy-efficient manner. To emulate the former, neuromorphic hardware typically leverages the conductance switching properties of thin-film dielectrics and semiconductors. Because these materials offer low ion mobilities, long-range connections built from thicker dielectrics require impractically-large forming voltages. To overcome this intrinsic shortcoming of solid-state active media, we present in this paper a simple Ag–H2O–Au cell that takes advantage of the relatively high ion mobility offered by deionized water to enable programmable connectivity switches between neurons separated by large gaps (∼40 µm). We introduce dual voltage programming schemes that allow the switch conductance to be modulated in analog and digital steps. When operating in the analog mode, the switch conductance could be potentiated and depressed over a relatively large (3.5×) range. In the digital mode, the Ag–H2O–Au switch delivered a high ON/OFF current ratio of ∼600 and sustained this margin over 200 switching cycles. Additionally, both switch states could be maintained for at least 3 h without external power. We show that unlike their solid-state counterparts, the water-gap in the Ag–H2O–Au cell can be easily refreshed without compromising the switching functionality. These attributes of Ag–H2O–Au switches in addition to their biocompatibility and simple design make them attractive for neuromorphic wetware implementations.

A series of equiatomic and non-equiatomic Fex(NiCrCo)100−x (at.-%, x = 25, 45, 55, 65, 75 and 85) multicomponent alloys were prepared and studied. With the increase in x, the phase structure of the alloys evolves from a single FCC phase (x = 25, 45 and 55), to a mixture of FCC and BCC phases (x = 55) and finally to a single BCC phase (x = 65 and 75). As a result, the BCC-structured alloys have much higher strength and hardness than the FCC-structured alloys. The existing VEC criteria are unable to predict the FCC-BCC phase transition in these alloys.

This paper mainly aimed at China's construction market downturn, current situation of building construction enterprise capital turnover difficult, seek countermeasure actively, think that the biggest problem facing China's construction enterprises at this stage is the source of the problem and funding problems, the so-called Chunwangchihan, construction enterprises must like replacing business tax with value-added tax(VAT) principle, to ensure the supply chain financing management. Support for upstream enterprises, and support for their own use of the upstream and downstream enterprises, this paper first through the traditional financing mode of construction enterprise limited to application of supply chain financing mode in construction enterprises are analyzed, finally the risk prevention in order to put forward some reasonable proposals. In order to solve the main problems of China's construction enterprises in the mode of application of supply chain financing, and help the downstream enterprises to exist in the reform trend of building market surging in the construction industry for the long-term, healthy, stable development of help.