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Introduction: Sparganii Rhizoma-Curcumae Rhizoma (SR-CR) formulation may offer an effective treatment for human colorectal cancer (CRC). However, the active ingredients and underlying mechanisms of this herbal formulation remain unclear. Methods: This study integrates network pharmacology, molecular docking and experimental verification to identify key active ingredients and elucidate the mechanisms of SR-CR in CRC treatment. Results: Twenty-three active components of SR-CR were identified using the TCMSP, UniProt and Gene Cards databases. These components were associated with 178 targets, of which 118 are directly related to CRC. Protein-protein interaction (PPI) network analysis identified protein kinase B (AKT) 1, epidermal growth factor receptor (EGFR), SRC, Bcl2 and CASP3 as core anti-CRC targets. Gene Ontology (GO) and KEGG pathway analyses were performed on these 178 intersecting targets, and the results showed that these targets are involved in EGFR tyrosine kinase inhibitor resistance and AGE-RAGE signaling pathway in diabetic complications. At the same time, five compounds, including bisdemethoxycurcumin, formononetin, β-sitosterol, stigmasterol and hederagenin, were selected based on the target number of effective components and tested for cytotoxicity against human CRC cell lines HT-29, HCT-8 and HCT-116 in vitro. The results showed that bisdemethoxycurcumin exhibited significant anti-proliferative activity against HCT-116 cells. Molecular docking results indicated that bisdemethoxycurcumin effectively binds with AKT, EGFR, Bcl-2 and CASP3. Further pharmacological experiments demonstrated that bisdemethoxycurcumin inhibits HCT-116 cell proliferation and migration via inhibiting EGFR-AKT pathway, and induces apoptosis by up-regulating Bcl-2 expression. Discussion: Based on our study, inhibiting the EGFR-AKT pathway and upregulating Bcl2 may be one of the mechanisms by which SR-CR inhibits the growth of CRC. The main active ingredients of SR-CR include bisdemethoxycurcumin, formononetin, β-sitosterol, stigmasterol and hederagenin, which may exert anti-colon cancer activity by targeting the regulation of AKT, EGFR, Bcl-2 and CASP3. However, we need more in vitro and in vivo evidence to confirm this conclusion. This study lays the foundation for further research on the pharmacological mechanism of SR-CR in the treatment of CRC.

Abstract Background Grapevine trunk diseases (GTDs) are disease complexes that are major threats to viticulture in most grapevine growing regions. The microbiomes colonizing plant belowground components form complex associations with plants, play important roles in promoting plant productivity and health in natural environments, and may be related to GTD development. To investigate associations between belowground fungal communities and GTD symptomatic or asymptomatic grapevines, fungal communities associated with three soil–plant compartments (bulk soils, rhizospheres, and roots) were characterized by ITS high-throughput amplicon sequencing across two years. Results The fungal community diversity and composition differs according to the soil–plant compartment type (PERMANOVA, p

Gray mold caused by Botrytis cinerea is a destructive disease in grapes. Although the preharvest use of pesticides can control it, fungicide resistance in B. cinerea is now common. We used an Effect and Less Spraying Control (ELSC) method for applying fungicides effective against B. cinerea on grapes. The spraying schedule was determined by exploring the key stages of B. cinerea invasion using field and in vitro inoculation tests. The results indicated that the stage most vulnerable to pathogen invasion is the full-bloom stage. The disease incidence/severity in this stage is highest compared with the pre-bloom, 10-days-after-full-bloom, bunch-closure and veraison stages. Given the inoculation results and the threat of residual infected petals, the ELSC method established an optimum spray schedule at full bloom and 10 days after full bloom. To evaluate the ELSC method, four kinds of fungicides were used in an experimental trial in Beijing in 2015 and 2017; Shanghai in 2016; and Hebei in 2019 and 2021. Fludioxonil was the most effective fungicide, followed by Pythium oligandrum (bio-fungicide), difenoconazole + azoxystrobin and pyraclostrobin. ELSC was more effective against B. cinerea than the traditional control schedule, when comparing the disease severity (i.e., 0.07 ± 0.10% in ELSC and 0.49 ± 0.014% in the traditional practice when using fludioxonil). The average yield per hectare in ELSC confirmed that spraying during flowering does not have a deleterious effect on grape yield. It produced a 1224.37 00 kg/ha greater yield than the control group when fludioxonil was applied.

This study aimed to identify fungal species associated with trunk diseases of sweet cherries (Prunus avium) in several commercial cherry orchards in Beijing, Guizhou and Shandong provinces, China. In total, eighteen fungal strains that fitted well into the species concept of Diaporthe were isolated. Based on both morphological and multi-locus phylogenetic analyses of internal transcribed spacer region (ITS), beta-tubulin (tub-2), calmodulin (Cal) and translation elongation factor 1-α (tef1–α) sequencing data, fourteen isolates were identified as Diaporthe eres, while four isolates were classified as D. hongkongensis. Here, we report D. hongkongensis causing sweet cherry branch dieback disease and, further, we confirmed the host association of D. eres with sweet cherries in China. A pathogenicity assay revealed the ability of both D. eres and D. hongkongensis to cause shoot necrosis and stem lesions on Prunus avium cv. ‘Brooks’ (mean lesion lengths of 1.86 cm and 1.56 cm, respectively). The optimal temperature for the growth of both Diaporthe species was tested. The optimal growth temperature for D. hongkongensis was 30 °C, and the 25–28 °C temperatures were the most favorable for the growth of D. eres strains. This research advances the understanding of fungal trunk diseases in fruit crops, particularly gummosis and branch dieback disease in Chinese cherry orchards, and will aid growers in making decisions about cultural practices and disease management.

Abstract The Esca disease complex includes some of the most important trunk diseases of grapevines (Vitis spp.) and causes serious yield losses in grape production worldwide. However, there has been no detailed study on its presence and associated pathogens in China. During 2017–2019, a preliminary field survey was conducted in eight vineyards in Hebei and Ningxia provinces, China when unusual foliar symptoms were observed. Symptoms were distinct tiger striped leaves, which are typical of grapevine leaf stripe disease (GLSD), one of the most common diseases in the Esca complex. Tiger-stripe leaf symptoms were found in four vineyards, and incidence was cultivar dependent varying with vineyard and year. A total of 266 fungal isolates were obtained from wood tissues of grapevines with typical foliar symptoms of GLSD. Based on morphological characters and multigene-combined phylogenetic analyses, the Ascomycete Phaeoacremonium minimum, one of the pathogens associated with Esca complex was identified. The basidiomycete Fomitiporia punicata, which has never been reported infecting grapevine, was also identified and found to be associated with wood rot in grapevine. The remaining isolates included some known wood pathogens, such as Neofusicoccum species and Diaporthe species. Koch’s postulates were performed in the greenhouse, confirming that both F. punicata and P. minimum caused leaf interveinal chlorosis and necrosis that resembled the GLSD symptoms of the Esca complex observed in the field. The present study provides the first detailed report of the Esca complex in China. In addition, this is the first record of F. punicata associated with Esca complex of grapevine worldwide. The results of this study provide new insights into the knowledge of the Esca complex.

Nigrospora is a monophyletic genus belonging to Apiosporaceae. Species in this genus are phytopathogenic, endophytic, and saprobic on different hosts. In this study, leaf specimens with disease symptoms were collected from host plants from the Shandong Peninsula, China. The fungal taxa associated with these leaf spots were studied using morphology and phylogeny based on ITS, TEF1, and TUB2 gene regions. In this article, we report on the genus Nigrospora with N. gorlenkoana, N. oryzae, N. osmanthi, N. rubi, and N. sphaerica identified with 13 novel host associations including crops with economic importance such as bamboo and Chinese rose.

Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in Lasiodiplodia theobromae and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system. The transcriptional levels of most CFEM genes, except for LtCFEM2 and LtCFEM6, were significantly elevated during infection. In addition, almost all LtCFEM genes, apart from LtCFEM2, LtCFEM3, and LtCFEM6, were transcriptionally up-regulated at 35°C in contrast to that at 25°C and 30°C. As two elicitors, LtCFEM1 induced local yellowish phenotype and LtCFEM4 triggered cell death in Nicotiana benthamiana leaves. Furthermore, these proteins displayed distinct subcellular localizations when expressed transiently in N. benthamiana. Moreover, two genes, LtCFEM7 and LtCFEM8, were found to be spliced alternatively by RT-PCR and sequencing. Therefore, our data suggest that LtCFEM proteins play important roles in multiple aspects, including pathogenicity and plant immune response, which will enhance our understanding of the sophisticated pathogenic mechanisms of plant opportunistic pathogen L. theobromae.

Sweet cherry is an important fruit crop in China with a high economic value. From 2019 to 2020, a leaf spot disease was reported, with purplish-brown circular lesions in three cultivating regions in China. Twenty-four Fusarium isolates were obtained from diseased samples and were identified based on morphological characteristics and multi-locus phylogenetic analyses. Seven species, including F. luffae (7 isolates), F. lateritium (6 isolates), F. compactum (5 isolates), F. nygamai (2 isolates), F. citri (2 isolates), F. ipomoeae (1 isolate) and F. curvatum (1 isolate) were identified. The pathogenicity test showed that analyzed strains of all species could produce lesions on detached cherry leaves. Therefore, Fusarium was proved to be a pathogen of cherry leaf spots in China. This is the first report of F. luffae, F. compactum, F. nygamai, F. citri, F. ipomoeae and F. curvatum on sweet cherry in China.

Grapevine trunk diseases (GTDs) are the most destructive diseases in grape-growing regions worldwide. Black foot is one of the important GTDs affecting young vineyards and nurseries. This disease has not been reported in China. During 2017 and 2019, field surveys were carried out in the Guangxi, Hebei, Ningxia, Shanxi, and Xinjiang provinces of China. Incidence of plants with black foot symptoms was 0.1% to 1% in the surveyed vineyards. Plant samples with poorly developed shoots and canes, chlorotic leaves, and necrotic trunks or roots were collected from the five provinces. In total, 50 fungal isolates were obtained from symptomatic tissues. Based on morphological and multi-gene phylogenetic analyses, five species were identified as Cylindrocladiella lageniformis, Dactylonectria torresensis, D. macrodidyma, D. alcacerensis and Neonectria sp.1. Pathogenicity was assessed using young, healthy detached green shoots of grapevine ‘Summer Black’ and potted 3-month-old ‘Summer Black’ cuttings. Inoculated detached shoots developed necroses after 7 d, and inoculated cuttings after 80 d. Fungi were re-isolated from necrotic lesions. Among the five species, D. macrodidyma was the most aggressive. This is the first report of C. lageniformis, D. torresensis, D. macrodidyma, D. alcacerensis, and Neonectria sp. 1 associated with black foot in China. This study has enhanced knowledge of the fungi associated with black foot in China, and will assist development of control measures for this disease.

The NmrA-like proteins have been reported to be important nitrogen metabolism regulators and virulence factors in herbaceous plant pathogens. However, their role in the woody plant pathogen Lasiodiplodia theobromae is less clear. In the current study, we identified a putative NmrA-like protein, Lws1, in L. theobromae and investigated its pathogenic role via gene silencing and overexpression experiments. We also evaluated the effects of external carbon and nitrogen sources on Lws1 gene expression via qRT-PCR assays. Moreover, we analyzed the molecular interaction between Lws1 and its target protein via the yeast two-hybrid system. The results show that Lws1 contained a canonical glycine-rich motif shared by the short-chain dehydrogenase/reductase (SDR) superfamily proteins and functioned as a negative regulator during disease development. Transcription profiling revealed that the transcription of Lws1 was affected by external nitrogen and carbon sources. Interaction analyses demonstrated that Lws1 interacted with a putative GATA family transcription factor, LtAreA. In conclusion, these results suggest that Lws1 serves as a critical regulator in nutrition metabolism and disease development during infection.

Lasiodiplodia theobromae is a causal agent of grapevine trunk disease, and it poses a significant threat to the grape industry worldwide. Fungal effectors play an essential role in the interaction between plants and pathogens. However, few studies have been conducted to understand the functions of individual effectors in L. theobromae. In this study, we identified and characterized a candidate secreted effector protein, LtCSEP1, in L. theobromae. Gene expression analysis suggested that transcription of LtCSEP1 in L. theobromae was induced at the early infection stages in the grapevine. Yeast secretion assay revealed that LtCSEP1 contains a functional signal peptide. Transient expression of LtCSEP1 in Nicotiana benthamiana suppresses BAX-trigged cell death and significantly inhibits the flg22-induced PTI-associated gene expression. Furthermore, the ectopic expression of LtCSEP1 in N. benthamiana enhanced disease susceptibility to L. theobromae by downregulating the defense-related genes. These results demonstrated that LtCSEP1 is a potential effector of L. theobromae, which contributes to suppressing the plant’s defenses.

A coelomycetous fungus with hyaline, aseptate, oblong to ellipsoidal conidia was isolated from living Chenopodium quinoa leaves with leaf spots, in Beijing, China. Maximum likelihood and Bayesian analyses of a combined LSU, SSU, ITS and TEF sequence dataset confirmed its placement in Heterosporicola in Leptosphaeriaceae. The new taxon resembles other Heterosporicola species, but is phylogenetically distinct, and is introduced as a new species. Heterosporicola beijingense sp. nov. is compared with other Heterosporicola species, and comprehensive descriptions and micrographs are provided.

The black-foot disease of grapevine is one of the most destructive diseases in viticulture and it is caused by a complex of soil-borne fungi. This study aimed to identify the species associated with black-foot disease in young grapevines in vineyards of China. Fungal isolates were identified as Campylocarpon fasciculare, based on both morphological and multi-locus phylogenetic analysis of ITS, tef1–α and ß-tubulin sequence data. For the first time in China, we report Campylocarpon fasciculare associated with symptomatic young grapevines. Koch’s postulates were performed on Vitis vinifera cv. Summer Black (SB) in a greenhouse and to confirm the pathogenicity on grapevines. This work improves the knowledge of black-foot disease in Chinese vineyards and will be helpful to growers in their decisions regarding vinicultural practices, planting and disease management.

Grapevine trunk diseases have become one of the main threats to grape production worldwide, with Diaporthe species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese Diaporthe populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize Diaporthe species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, Diaporthe guangxiensis (D. guangxiensis), Diaporthe hubeiensis (D. hubeiensis), Diaporthe viniferae (D. viniferae), and three new host records, Diaporthe gulyae (D. gulyae), Diaporthe pescicola (D. pescicola), and Diaporthe unshiuensis (D. unshiuensis). The most commonly isolated species was Diaporthe eres (D. eres). In addition, high genetic diversity was observed for D. eres in Chinese vineyards. Haplotype network analysis of D. eres isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, D. gulyae was the most aggressive taxon, whereas D. hubeiensis was the least aggressive. This study provides new insights into the Diaporthe species associated with grapevines in China, and our results can be used to develop effective disease management strategies.

Long non-coding RNAs (lncRNAs) refer to a class of RNA molecules that are longer than 200 nucleotides and do not encode proteins. Numerous lncRNAs have recently emerged as important regulators of many biological processes in animals and plants, including responses to environmental stress and pathogens. Botryosphaeria dieback is one of the more severe grapevine trunk diseases worldwide. However, how lncRNAs function during Botryosphaeriaceae infection is largely unknown. We performed high-throughput RNA-sequencing (RNA-seq) of susceptible and more tolerant grapevine cultivars infected with Lasiodiplodia theobromae . Overall, we predicted 1826 novel candidate lncRNAs, including long intergenic non-coding RNAs (lincRNAs) and natural antisense transcripts (lncNATs). The data reveal the functions of a set of lncRNAs that were differentially expressed between the resistant cultivar Merlot and the susceptible cultivar Cabernet Franc. Several lncRNAs were predicted to be precursors for grape microRNAs involved in the L theobromae infection. These results provide new insight into the lncRNAs of grapevine that are involved in the response to L theobromae infection.

The family Botryosphaeriaceae is species rich and includes pathogens, saprobes and endophytes of economically important agricultural crops and plants. As pathogens, Botryosphaeriaceae species employ evolving defensive and counter-defensive strategies that enable them to infect their hosts. Molecular genetic results showed high genetic variability among Botryosphaeriaceae species, which could be a key determinant to their differing environmental roles on hosts and differences in their virulence. Improved technologies such as whole genome re-sequencing, integrated RNA-Seq, comparative and ab initio approaches for molecular genetics and mutation analysis have revealed possible virulence factors that might be involved in the pathogenicity of these fungi. Several genes involved in the pathogenicity processes of botryosphaeriaceous fungi have been identified and characterized. There are numerous reports on the involvement of phytotoxic metabolites in the pathogenicity of these taxa. Availability of whole genomes, genetic transformation and transcriptome analysis of some botryosphaeriaceous species have contributed towards identifying the biological functions of many genes in a fast and accurate way, enabling these fungi to be used as model organism for molecular research of plant opportunistic fungal pathogens in woody plants. The research on botryosphaeriaceous species will provide understanding of the infection mechanisms, and with designing control strategies against diseases caused by opportunistic fungal pathogens.

The Botryosphaeriaceae represents an important, cosmopolitan family of pathogens infecting woody plants. Grapevines (Vitis vinifera) suspected of being affected by Botryosphaeria dieback were collected from different Provinces of China and several Botryosphaeriaceae species were identified. This research was conducted to further study the species of Botryosphaeriaceae associated with grapevines in China and to estimate the prevalence and severity of the disease. Symptoms were characterized by partial or total death of grapevine cordons, with brown U-shaped necrotic sectors and brownish-black spots in cross-sections of affected trunks and arms. A fungus isolated from diseased vines in Henan and Anhui Provinces was identified as Neofusicoccum mangiferae on the basis of its morphological and cultural characteristics as well as an analysis of combined sequence data from the internal transcribed spacer (ITS) cluster of the ribosomal DNA together with partial sequences of the β-tubulin (BT) and translation elongation factor (EF1-α) genes. Pathogenicity was assessed by inoculating the fungus onto detached green grapevine shoots under controlled laboratory conditions and mature wood of standing vines cv. Summer Black in a greenhouse. This is the first record of N. mangiferae associated with grapevine dieback in the world.

Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related genes. Lasiodiplodia theobromae strain JZB 0300251, a highly virulent isolate, was selected for the genetic transformation. Based on optimised conditions, the Restriction Enzyme-Mediated Integration (REMI) methodology was established in L. theobromae using pUCATPH (a plasmid carrying a hygromycin B marker). A total of 6,036 transformants were generated with four restriction enzymes, respectively and the transformant library was evaluated based on 200 randomly selected transformants. Mutants that exhibited various degrees of virulence and different growth rates were obtained. The study provides basic results that will lead to increased understanding of the role of the pathogenicity-related genes involved in the infection process of L. theobromae.

ObjectiveTo study the effects of sodium cantharidinate (SC) on the proliferation and apoptosis of gastric cancer cells through JAK2/ STAT3 pathway. MethodsGastric cancer cell line SGC-7901 was cultured and treated with different concentrations of SC (0.25, 0.5, 1.0, 2.0, 4.0, 8.0, and 16.0 μmol/L) and then transfected with control plasmid or JAK2 plasmid. Cell survival rate, apoptosis rate, and the expression levels of p-JAK2, p-STAT3, p-p38, p-ERK, and p-JNK were detected after 48 h of treatment. ResultsThe results indicated that 1.0, 2.0, 4.0, 8.0, and 16.0 μmol/L of SC inhibited cell proliferation, and the survival rate decreased with an increase in SC concentration (P0.05), while those of the 2.0 and 4.0 μmol/L SC groups increased significantly (P0.05) in the 1.0, 2.0, and 4.0 μmol/L SC groups. The JAK2 plasmid was transfected simultaneously with the 4.0 μmol/L SC treatment; the expression levels of p-JAK2 and p-STAT3 and the survival rate increased, whereas the apoptosis rate decreased (P

A pot experiment was carried out to determine the effect of Serendipita indica on the salt response of Lolium multiflorum Lam. Although the salinity decreased the root colonisation of S. indica by 28.34%, successful colonisation of S. indica increased the seed germination rate, fresh weight, leaf relative water content and chlorophyll content by 28.09, 59.01, 38.78 and 28.80%, respectively, compared with uncolonised seedlings. Under salinity, leaf malondialdehyde content, leaf relative electrical conductivity, as well as Na+ content and Na+/K+ ratio in leaves and roots of S. indica-colonised seedlings were decreased by 33.99, 33.31, 63.40% and 47.42, 85.66 and 55.88%, respectively, compared with uncolonised seedlings. Meanwhile, compared with uncolonised seedlings under salinity, the contents of proline in leaves, N, P and K+ in leaves and roots of the S. indica-colonised seedlings were increased by 47.47, 45.69 and 30.05%, and 41.77, 19.51, 19.18 and 155.00%, respectively. These results indicate that S. indica colonisation confers salt tolerance in L. multiflorum seedlings by enhancing osmotic adjustment via actively accumulating proline and K+, increasing the uptake of nutrients such as N and P, and improving Na+/K+ homoeostasis. The study would provide a new idea for the combined application of salt-tolerant plants and symbiotic microorganisms in the ecological restoration of saline-alkali lands.

Water scarcity, over-fertilization, and improper crop management practices severely limit the sustainable cultivation of licorice (Glycyrrhiza uralensis Fisch) in the arid regions of Xinjiang. To elucidate the impacts of integrated water and fertilizer management on the growth characteristics and bioactive components (glycyrrhizic acid and liquiritin) of four-year-old licorice plants, a comprehensive four-year field experiment was conducted from 2019 to 2022.The experiment included four irrigation levels (W1: 2500 m³/ha, W2: 4000 m³/ha, W3: 5500 m³/ha, W4: 7000 m³/ha) and four fertilization rates (F1: 305 kg/ha, F2: 610 kg/ha, F3: 915 kg/ha, F4: 1220 kg/ha), following a completely randomized design. Results indicated that both irrigation and fertilization significantly influenced plant height, root length, root weight, root diameter, leaf area index, and root-to-shoot ratio. The optimal growth characteristics were observed under the W2F2 treatment. The contents of glycyrrhizic acid and liquiritin varied significantly among different water and fertilizer treatments, with the highest levels observed under the W2F2 treatment. Excessive irrigation (W4) and over-fertilization (F4) led to a decrease in these bioactive components. A comprehensive evaluation of the growth characteristics and bioactive components revealed that the ideal irrigation and fertilization parameters were 4000 m³/ha and 610 kg/ha, respectively. These parameters optimized plant development and bioactive component accumulation while ensuring efficient resource use. This study provides scientific evidence for optimizing irrigation and fertilization strategies to enhance licorice yield in arid regions, thereby supporting sustainable agricultural practices and improving economic benefits.

Current research on the stellate ganglion (SG) has shifted from merely understanding its role as a collection of neurons to recognizing its importance in immune regulation. As part of the autonomic nervous system (ANS), the SG plays a crucial role in regulating cardiovascular function, particularly cardiac sympathetic nerve activity. Abnormal SG function can lead to disordered cardiac electrical activity, which in turn affects heart rhythm stability. Studies have shown that excessive activity of the SG is closely related to the occurrence of arrhythmias, especially in the context of inflammation. Abnormal activity of the SG may trigger excessive excitation of the sympathetic nervous system (SNS) through neuroimmune mechanisms, thereby increasing the risk of arrhythmias. Simultaneously, the inflammatory response of the SG further aggravates this process, forming a vicious cycle. However, the causal relationship between SG, inflammation, and arrhythmias has not yet been fully clarified. Therefore, this article deeply explores the key role of the SG in arrhythmias and its complex relationship with inflammation, providing relevant clinical evidence. It indicates that interventions targeting SG function and inflammatory responses have potential in preventing and treating inflammation-related arrhythmias, offering a new perspective for cardiovascular disease treatment strategies.

IntroductionOur objective was to explore the efficacy of hyperbaric oxygen in the treatment of sudden sensorineural hearing loss by conducting an umbrella review of all existing evidence.MethodsWe conducted an umbrella review, searching for related articles in the PubMed, Web of Science, Embase, and Scopus databases. The search period covered from the inception of each database until April 2024. We extracted authors, country of publication, time of publication, number of included studies and participants, interventions, summary of results, P-values, I2, relative risk (95% CI), and outcome measures. The methodological quality, evidence quality, and overlap rate of the included articles were assessed using AMSTAR 2, GRADE, and OVErviews (GROOVE).ResultsMethodological quality was assessed using AMSTAR 2. Of the nine included articles, two were assessed as “high,” three as “moderate,” two as “low,” and the remaining two as “very low.” The quality of evidence was assessed using the GRADE system. It was found that the quality of evidence in most of the studies was unsatisfactory. It was found that there was a slight overlap among the included articles. Six studies reported positive results (OR 1.37; 95% CI, 1.17–1.61; P = 0.04), with high heterogeneity observed (I2 = 63%). Egger's test indicated bias (P = 0.000101). Three studies reported negative results (MD 1.49; 95% CI, −0.32 to 3.29; P = 0.43; I2 = 0%), with no significant bias detected (P = 0.106) according to Egger's test.ConclusionHBO therapy is shown to be an effective treatment for SSNHL with fewer side effects. However, the methodological quality and evidence of the systematic reviews and meta-analysis included in this study were generally low. Therefore, more high-quality, large-scale, multi-center randomized controlled trials are needed in the future to verify the efficacy of HBO therapy for SSNHL.Systematic review registrationhttps://www.crd.york.ac.uk/prospero, identifier [CRD42024523651].