113 results on '"Xing XY"'
Search Results
2. Novel cytokine and chemokine markers of hidradenitis suppurativa reflect chronic inflammation and itch
- Author
-
Vossen, Allard, van der Zee, Hessel, Tsoi, L C, Xing, XY, Devalaraja, M, Gudjonsson, JE, Prens, Errol, Vossen, Allard, van der Zee, Hessel, Tsoi, L C, Xing, XY, Devalaraja, M, Gudjonsson, JE, and Prens, Errol
- Published
- 2019
3. Genome-Wide Expression Profiling of Five Mouse Models Identifies Similarities and Differences with Human Psoriasis
- Author
-
Swindell, WR, Johnston, A, Carbajal, S, Han, GW, Wohn, Christian, Lu, J, Xing, XY, Nair, RP, Voorhees, JJ, Elder, JT, Wang, XJ, Sano, S, Prens, Errol, DiGiovanni, J, Pittelkow, MR, Ward, NL, Gudjonsson, JE, Swindell, WR, Johnston, A, Carbajal, S, Han, GW, Wohn, Christian, Lu, J, Xing, XY, Nair, RP, Voorhees, JJ, Elder, JT, Wang, XJ, Sano, S, Prens, Errol, DiGiovanni, J, Pittelkow, MR, Ward, NL, and Gudjonsson, JE
- Abstract
Development of a suitable mouse model would facilitate the investigation of pathomechanisms underlying human psoriasis and would also assist in development of therapeutic treatments. However, while many psoriasis mouse models have been proposed, no single model recapitulates all features of the human disease, and standardized validation criteria for psoriasis mouse models have not been widely applied. In this study, whole-genome transcriptional profiling is used to compare gene expression patterns manifested by human psoriatic skin lesions with those that occur in five psoriasis mouse models (K5-Tie2, imiquimod, K14-AREG, K5-Stat3C and K5-TGFbeta1). While the cutaneous gene expression profiles associated with each mouse phenotype exhibited statistically significant similarity to the expression profile of psoriasis in humans, each model displayed distinctive sets of similarities and differences in comparison to human psoriasis. For all five models, correspondence to the human disease was strong with respect to genes involved in epidermal development and keratinization. Immune and inflammation-associated gene expression, in contrast, was more variable between models as compared to the human disease. These findings support the value of all five models as research tools, each with identifiable areas of convergence to and divergence from the human disease. Additionally, the approach used in this paper provides an objective and quantitative method for evaluation of proposed mouse models of psoriasis, which can be strategically applied in future studies to score strengths of mouse phenotypes relative to specific aspects of human psoriasis.
- Published
- 2011
4. Identification of New β-Delayed γ Raysand a Low-Spin Isomer in 176Ir.
- Author
-
Wang WH Hua-Lei, Zhang ZY Yu-Hu, Zhou ZX Xiao-Hong, Guo GY Ying-Xiang, Lei LX Xiang-Guo, Xu XS Shu-Wei, Xie XY Yuan-Xiang, Liu LM Min-Liang, Zheng ZY Yong, Xing XY Ye-Bing, Xie XC Cheng-Ying, Song SL Li-Tao, Luo LP Peng, Yu YH Hai-Ping, and Guo GW Wen-Tao
- Published
- 2005
5. Identification of New β-Delayed γ Rays and a Low-Spin Isomer in 176Ir
- Author
-
Hua-Lei, Wang WH, Yu-Hu, Zhang ZY, Xiao-Hong, Zhou ZX, Ying-Xiang, Guo GY, Xiang-Guo, Lei LX, Shu-Wei, Xu XS, Yuan-Xiang, Xie XY, Min-Liang, Liu LM, Yong, Zheng ZY, Ye-Bing, Xing XY, Cheng-Ying, Xie XC, Li-Tao, Song SL, Peng, Luo LP, Hai-Ping, Yu YH, and Wen-Tao, Guo GW
- Abstract
The β+/EC decay of doubly odd nucleus 176Ir has been studied via the 146Nd(35Cl, 5nγ) heavy ion fusion evaporation reaction at 210 MeV bombarding energy. With the aid of a helium-jet recoil fast tape transport system, the reaction products were transported to a low-background location for measurement. Based on the data analysis, the previously known γ rays from the decay of 176Ir are proven. Moreover, three new excited levels and ten new γ rays are assigned to 176Os. The time spectra of typical γ rays clearly indicate a long-lived low-spin isomer in 176Ir.
- Published
- 2005
6. A case of extremely high serum CA19-9 level without any inducement.
- Author
-
Qiu X, Liu W, Xing XY, and Chen LJ
- Abstract
Competing Interests: Declaration of competing interest The authors declared no competing interests.
- Published
- 2024
- Full Text
- View/download PDF
7. Computational Simulation Study of Potential Inhibition of c-Met Kinase Receptor by Phenoxy pyridine Derivatives: Based on QSAR, Molecular Docking, Molecular Dynamics.
- Author
-
Guo LY, Yang YL, Tong JB, Chang ZL, Gao P, Liu Y, Zhang YK, and Xing XY
- Subjects
- Humans, Drug Design, Molecular Structure, Pyridazines chemical synthesis, Pyridazines chemistry, Pyridazines pharmacology, Molecular Docking Simulation, Molecular Dynamics Simulation, Protein Kinase Inhibitors chemistry, Protein Kinase Inhibitors pharmacology, Proto-Oncogene Proteins c-met antagonists & inhibitors, Proto-Oncogene Proteins c-met metabolism, Pyridines chemistry, Pyridines pharmacology, Pyridines chemical synthesis, Quantitative Structure-Activity Relationship
- Abstract
The mesenchymal-epithelial transition factor (c-Met) is a tyrosine kinase receptor protein, and excessive cell transformation can lead to cancer. Therefore, there is an urgent need to develop novel receptor tyrosine kinase inhibitors by inhibiting the activity of c-Met protein. In this study, 41 compounds are selected from the reported literature, and the interactions between phenoxy pyridine derivatives and tumor-associated proteins are systematically investigated using a series of computer-assisted drug design (CADD) methods, aiming to predict potential c-Met inhibitors with high activity. The Topomer CoMFA (q
2 =0.620, R2 =0.837) and HQSAR (q2 =0.684, R2 =0.877) models demonstrate a high level of robustness. Further internal and external validation assessments show high applicability and accuracy. Based on the results of the Topomer CoMFA model, structural fragments with higher contribution values are identified and randomly combined using a fragment splice technique, result in a total of 20 compounds with predicted activities higher than the template molecules. Molecular docking results show that these compounds have good interactions and van der Waals forces with the target proteins. The results of molecular dynamics and ADMET predictions indicate that compounds Y4, Y5, and Y14 have potential as c-Met inhibitors. Among them, compound Y14 exhibits superior stability with a binding free energy of -165.18 KJ/mol. These studies provide a reference for the future design and development of novel compounds with c-Met inhibitory activity., (© 2024 Wiley-VHCA AG, Zurich, Switzerland.)- Published
- 2024
- Full Text
- View/download PDF
8. Study on the anti-HBV activity of matrine alkaloids from Oxytropis ochrocephala by MTT, 3d-QSAR, molecular docking and molecular dynamics simulation.
- Author
-
Zhang YK, Tong JB, Tan J, Yang M, Xing XY, Zeng YR, Xue Z, and Tan CJ
- Abstract
To elucidate the structure-activity relationship of 17 matrine alkaloids from Oxytropis ochrocephala Bunge, their effect on hepatitis B surface antigen (HBsAg) secretion was studied using the MTT assay. A 3D-QSAR analysis showed a strong correlation between chemical structures and biological activities ( q
2 = 0.625, r2 = 0.859). Molecular docking and molecular dynamics simulations revealed that hydrogen bonding and hydrophobic interactions with hepatitis B core protein (PDB:5T2P) are key to inhibiting HBsAg secretion, suggesting potential for developing natural anti-hepatitis B drugs.- Published
- 2024
- Full Text
- View/download PDF
9. Current situation and influencing fctors of oral frailty for community-dwelling older adults in the northeastern border areas of China: A cross-sectional study.
- Author
-
Wang GM, Xing XY, Xia ZH, Yu WJ, Ren H, Teng MY, and Cui XS
- Abstract
Objectives: Unique lifestyle and cultural factors in China may lead to distinct patterns of risk factors for oral frailty among older adults, especially in regions inhabited by northeastern border minority groups., Methods: From July to November 2023, a convenience sampling method was employed to select older adults from three communities in Yanji City as the subjects. Data were collected by a set of questionnaires., Results: A total of 478 older adults were included, revealing a prevalence rate of 71.6 % for oral frailty. Factors influencing were found to include age, ethnicity, gender, income, number of chronic diseases, body mass index, drinking, physical frailty, sleep disorders, and attitudes towards aging (p < 0.05)., Conclusions: There is a higher prevalence of oral frailty. It is crucial to prioritize the oral health issues of older adults with high-risk factors and implement targeted intervention measures to reduce and control the occurrence and progression of oral frailty., Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
10. Accuracy of cell-free Mycobacterium tuberculosis DNA testing in pleural effusion for diagnosing tuberculous pleurisy: a multicenter cross-sectional study.
- Author
-
Du WL, Liang JQ, Yang XT, Li CJ, Wang QF, Han WG, Li Y, Li ZH, Zhao DM, Xu FD, Rong YX, Cui XJ, Li HM, Wang F, Liu PC, Guo DL, Wang HB, Xing XY, Che JL, Liu ZC, Zhang NN, Li K, Liu Y, Wang L, Wang HB, and Che NY
- Subjects
- Humans, Female, Cross-Sectional Studies, Male, Middle Aged, Adult, China, Cell-Free Nucleic Acids analysis, Aged, Sensitivity and Specificity, Tuberculosis, Pleural diagnosis, Mycobacterium tuberculosis genetics, Pleural Effusion microbiology, Pleural Effusion diagnosis, DNA, Bacterial analysis
- Abstract
Background: The diagnosis of tuberculous pleurisy (TP) presents a significant challenge due to the low bacterial load in pleural effusion (PE) samples. Cell-free Mycobacterium tuberculosis DNA (cf-TB) in PE samples is considered an optimal biomarker for diagnosing TP. This study aimed to evaluate the applicability of cf-TB testing across diverse research sites with a relatively large sample size., Methods: Patients suspected of TP and presenting with clinical symptoms and radiological evidence of PE were consecutively enrolled by treating physicians from 11 research sites across 6 provinces in China between April 2020 and August 2022. Following centrifugation, sediments obtained from PE were used for Xpert MTB/RIF (Xpert) and mycobacterial culture, while the supernatants were subjected to cf-TB testing. This study employed a composite reference standard to definite TP, which was characterized by any positive result for Mycobacterium tuberculosis (MTB) through either PE culture, PE Xpert, or pleural biopsy., Results: A total of 1412 participants underwent screening, and 1344 (95.2%) were subsequently enrolled in this study. Data from 1241 (92.3%) participants were included, comprising 284 with definite TP, 677 with clinically diagnosed TP, and 280 without TP. The sensitivity of cf-TB testing in definite TP was 73.6% (95% CI 68.2-78.4), significantly higher than both Xpert (40.8%, 95% CI 35.3-46.7, P < 0.001) and mycobacterial culture (54.2%, 95% CI 48.4-59.9, P < 0.001). When clinically diagnosed TP was incorporated into the composite reference standard for sensitivity analysis, cf-TB testing showed a sensitivity of 46.8% (450/961, 95% CI 43.7-50.0), significantly higher than both Xpert (116/961, 12.1%, 95% CI 10.2-14.3, P < 0.001) and mycobacterial culture (154/961, 16.0%, 95% CI 13.8-18.5, P < 0.001). The specificities of cf-TB testing, Xpert, and mycobacterial culture were all 100.0%., Conclusions: The performance of cf-TB testing is significantly superior to that of Xpert and mycobacterial culture methods, indicating that it can be considered as the primary diagnostic approach for improving TP detection. Trial registration The trial was registered on Chictr.org.cn (ChiCTR2000031680, https://www.chictr.org.cn/showproj.html?proj=49316 )., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
11. [Etiology, Clinical Characteristics and Prognosis of Secondary Hemophagocytic Syndrome].
- Author
-
Zhang YL, Hao JN, Sun MM, Xing XY, and Qiao SK
- Subjects
- Humans, Prognosis, Retrospective Studies, Male, Female, Adult, Lymphoma complications, Lymphoma diagnosis, Lymphohistiocytosis, Hemophagocytic diagnosis, Lymphohistiocytosis, Hemophagocytic etiology
- Abstract
Objective: To understand the etiology, clinical characteristics and prognosis of secondary hemophagocytic syndrome (HLH), so as to improve the understanding of HLH and reduce the rates of misdiagnosis and missed diagnosis of HLH., Methods: A retrospective study was conducted to analyze the cause, clinical characteristics, laboratory findings, therapy and outcomes of 75 adult patients with secondary HLH admitted to our hospital from January 2015 to December 2021. Follow-up continued until the last discharge time., Results: Among 75 patients, infection-related HLH was the most common (45.33%), followed by lymphoma-related HLH (17.33%). Fever was the most common clinical manifestation (97.67%). Laboratory indicators such as NK cell activity (98.31% low or absent), sCD25 (93.22% increased), and serum ferritin (94.44% elevated) had higher sensitivity in diagnosis. By comparing the clinical manifestations and laboratory indicators of HLH patients with different causes, sex, lymph node enlargement and bone marrow morphology were more valuable for the diagnosis of primary disease (all P <0.05). By comparing the treatment and clinical outcomes of HLH patients with different causes, the highest clinical remission rate (83.3%) was achieved in patients with autoimmune disease-related HLH treated with hormone+cyclosporine ( P <0.05). The overall 12-month survival rate of all patients was 26.7%, in which the infection-related HLH was the lowest (14.7%) while autoimmune disease-related HLH was the highest (63.6%)., Conclusion: The causes and clinical characteristics of adult secondary HLH are varied, with poor prognosis and heterogeneity in disease severity. It is important to identify HLH cause early for diagnosis and needed to further understand HLH.
- Published
- 2024
- Full Text
- View/download PDF
12. Current status and influencing factors of test anxiety of senior one students in Yanji, China: a cross-sectional study.
- Author
-
Xing XY, Wang GM, Li Y, Zhang WX, and Shen XD
- Abstract
Objective: Examining the current situation of test anxiety among first year senior high school students in Yanji City and investigating the factors that contribute to exam anxiety., Methods: Using cluster sampling, a survey was conducted on 1,550 first-year high school students from three high schools in Yanji City in April-May 2023. The survey utilized general information questionnaires, the Minnesota Multiphasic Personality Inventory (MMPI), and the Self-Rating Anxiety Scale (SAS). Logistic regression analysis was used to determine the influencing factors of test anxiety., Results: A total of 1,550 first-year high school students were included in the analysis, with a test anxiety occurrence rate of 79.8%. Test anxiety exhibited statistical differences among different genders, ethnicities, family economic levels, frequency of communication with parents, and relationships with parents (with results of 53.44, 10.42, 17.31, 20.42, 31.95, all p < 0.05). Scores of hypochondriasis (Hs), depression (D), psychasthenia (Pt), paranoia (Pa), psychopathic deviate (Pd), schizophrenia (Sc), and hypomania (Ma) in the 10 clinical personality scales were significantly positively correlated. Logistic regression analysis revealed that gender, ethnicity, frequency of communication with parents, and scores of hypochondriasis (Hs), depression (D), psychasthenia (Pt), paranoia (Pa), and hypomania (Ma) in the 10 clinical personality scales were the main influencing factors for test anxiety in first-year high school students (all p < 0.05)., Conclusion: The test anxiety level of high school students in Yanji City is relatively high, with variations in test anxiety levels among students of different genders, ethnicities, parental communication styles, and deviant personality traits. It is recommended that schools and teachers should give more consideration to test anxiety among high school students, particularly targeting those with potential risk factors. Parents should also be more attentive to their children's development and advancement, and improve their family education principles., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Xing, Wang, Li, Zhang and Shen.)
- Published
- 2024
- Full Text
- View/download PDF
13. Effect of electroacupuncture on myocardial transient receptor potential channels in mice with myocardial hypertrophy.
- Author
-
Xie YH, Xing XY, Zhong W, Wu SB, Zhou MQ, and Cui S
- Subjects
- Animals, Mice, Male, Humans, Transient Receptor Potential Channels metabolism, Transient Receptor Potential Channels genetics, Cardiomegaly metabolism, Cardiomegaly therapy, Cardiomegaly genetics, Cardiomegaly physiopathology, Acupuncture Points, TRPC Cation Channels metabolism, TRPC Cation Channels genetics, Electroacupuncture, Mice, Inbred C57BL, Myocardium metabolism
- Abstract
Objectives: To observe the effect of electroacupuncture (EA) at "Neiguan"(PC6) on cardiac function, cardiac morphology and transient receptor potential channel (TRPC) protein expressions in myocardial tissue of mice with myocardial hypertrophy, so as to explore its mechanisms underlying improvement of myocardial hypertrophy., Methods: Forty-five male C57BL/6 mice were randomly divided into control, model and EA groups (15 mice/group). The myocardial hypertrophy model was established by subcutaneous injection of isoproterenol hydrochloride (15 mg·kg
-1 ·d-1 ) for 14 days. The mice of the control group received subcutaneous injection of same amount of normal saline. The mice of the EA group received EA stimulation (frequency of 2 Hz, intensity of 1 mA) of bilateral PC6 for 20 min each time, once a day for 14 consecutive days. After the intervention, the body weight, tibia length and heart weight were measured. The left ventricular ejection fraction (EF), fractional shortening index (FS), left ventricular end-systolic volume (LVEV), left ventricular end-systolic internal diameter (LVID) and left ventricular posterior wall thickness (LVPW) were measured by using echocardiography for evaluating the cardiac function. The mean number and surface area of myocardial cells was detected by wheat germ agglutinin (WGA) staining, and changes of the cardiac morphology were observed under light microscopy after HE staining. The expression levels of TRPC1, TRPC3, TRPC4 and TRPC6 (TRPC1/3/4/6) in the myocardial tissue were detected by real-time quantitative PCR (qPCR) and Western blot, separately., Results: Compared with the control group, the heart-body weight ratio( P <0.05) and heart-weight-to-tibia-length ratio ( P <0.01), LVEV and LVID levels, the relative surface area, left ventricular area ratio, and the expression levels of cardiac TRPC1/3/4/6 were significantly increased ( P <0.01, P <0.05), while the EF, FS, LVPW, number of cardiomyocytes, and the left ventricular posterior wall ratio were obviously decreased ( P <0.01, P <0.05) in the model group. In comparison with the model group, the heart/body weight ratio, heart-weight-to-tibia-length ratio, LVEV and LVID levels, relative surface area, left ventricular area ratio, and the expression levels of cardiac TRPC1/3/4/6 were significantly decreased ( P <0.01, P <0.05), while the EF, FS, LVPW, number of cardiomyocytes and left ventricular posterior wall ratio were significantly increased ( P <0.01, P <0.05) in the EA group. H.E. staining showed disordered arrangement of cardiomyocytes and obvious myocardial interstitial inflammatory cell infiltration in the model group, and evident reduction of degree of cardiac fibrosis and interstitial edema in the EA group., Conclusions: EA of PC6 can improve the cardiac function and cardiac morphology in mice with myocardial hypertrophy, which may be related to its functions in down-regulating the expression of transient receptor potential channels.- Published
- 2024
- Full Text
- View/download PDF
14. The awareness rate of knowledge of chronic diseases and influencing factors among 4790 adults in anhui province: An online survey using WeChat.
- Author
-
Xing XY, Wu ZZ, Wang HD, Xu W, Cao D, Liu ZR, and Wu GC
- Abstract
Objective: To investigate public awareness about core information regarding chronic diseases and identify factors influencing that awareness among Anhui Province residents, provide a scientific basis for policy-making, and formulate corresponding intervention measures., Methods: From March to April 2021, 12 provincial-level representative counties and districts of Anhui province in the China Adult Chronic Disease and Nutrition Surveillance were selected as survey sites, and 4790 residents were recruited for the survey using stratified multi-stage cluster random sampling. Basic details about the study participants were collected and their awareness of core information about major chronic diseases was measured through an online survey using WeChat., Results: In 2021, the awareness rate of core information about chronic diseases among residents of Anhui Province was 54.93%. Multivariate logistic regression analysis showed that a higher awareness rate was associated with the following factors: non-housework occupations (agriculture, forestry, animal husbandry, and fishery: OR = 1.309, commercial services and production and transportation: OR = 1.450, institutions, and professional and technical personnel: OR = 1.461), a high education level (high school/junior high school/technical school OR = 1.357, college and above OR = 2.133), and residence in the southern and northern Anhui areas (southern Anhui OR = 1.282, northern Anhui OR = 1.431); whereas in rural areas (by district and country) (OR = 0.863), the awareness rate was low (all P < 0.05)., Conclusions: The awareness rate of core information about chronic diseases among residents of Anhui, China, is low. It is necessary to strengthen awareness about chronic disease prevention and management by targeting specific groups of people in this region., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)
- Published
- 2024
- Full Text
- View/download PDF
15. Impacts of spatial symmetries on stimulated Brillouin scatterings in nanoscale silicon waveguides: a theoretical and numerical study considering material anisotropies.
- Author
-
Xing XY, Su XX, Shui GS, Qin J, and Lee HP
- Abstract
Fully considering the mechanical and photoelastic anisotropies of monocrystalline silicon, the impacts of spatial symmetries on the stimulated Brillouin scatterings (SBSs) in nanoscale suspended silicon waveguides are studied theoretically and numerically based on group theory. First, starting from an assumption that the principal material coordinate system can be arbitrarily orientated in a waveguide with fixed geometry, the silicon waveguides are systematically classified into a number of point groups according to their spatial symmetry features. Thereafter, the symmetry characteristics of physical fields and SBS opto-mechanical coupling characteristics in the silicon waveguides belonging to different point groups are further examined, and the major new findings can be summarized as follows: The SBS opto-mechanical couplings in several kinds of silicon waveguides with certain nontrivial symmetry features exhibit relatively predictable behaviors in that the opto-mechanical coupling coefficients can be deterministically vanishing or nonvanishing under very few constraints, which can thus serve as general symmetry selection rules for SBSs in suspended silicon waveguides. The results obtained in the present study could be a useful theoretical reference for the design of novel SBS-active silicon photonic devices.
- Published
- 2024
- Full Text
- View/download PDF
16. Clinical analysis of 12 cases of ovarian neuroendocrine carcinoma.
- Author
-
Xing XY, Zhang W, Liu LY, and Han LP
- Abstract
Background: Neuroendocrine neoplasms of the female genital tract are rare., Aim: To enhance our clinical understanding of neuroendocrine carcinoma (NEC) of the ovary., Methods: A retrospective review was conducted on 12 patients diagnosed with NEC of the ovary, analyzing clinicopathological characteristics, treatment modalities, and survival status., Results: The median age at diagnosis was 34.5 years (range: 20 to 62 years). Among the 12 cases, 9 were small cell carcinoma of the ovary and 3 were large cell NEC. Five cases were stage I tumors, one case was stage IV, and six cases were stage III. Eleven patients underwent surgery as part of their treatment. All patients received adjuvant chemotherapy. Among the 12 patients, one patient received radiotherapy, and one patient with a BRCA2 mutation was administered PARP inhibitor maintenance after chemotherapy. The median progression-free survival was 13 months, and the median overall survival was 19.5 months. Four cases remained disease-free, while eight cases experienced tumor recurrence, including three cases that resulted in death due to disease recurrence., Conclusion: NEC of the ovary is a rare condition that is more common in women of childbearing age and is associated with aggressive behavior and poor clinical outcomes. Surgical resection remains the mainstay of treatment, with some patients benefiting from adjuvant chemoradiation therapy., Competing Interests: Conflict-of-interest statement: All the authors declare that they have no conflict of interest., (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
17. Peroxiredoxin II regulates exosome secretion from dermal mesenchymal stem cells through the ISGylation signaling pathway.
- Author
-
Han YH, Mao YY, Lee KH, Cho HJ, Yu NN, Xing XY, Wang AG, Jin MH, Hong KS, Sun HN, and Kwon T
- Subjects
- Peroxiredoxins metabolism, Signal Transduction, Exosomes metabolism, Mesenchymal Stem Cells metabolism, MicroRNAs metabolism
- Abstract
Background: Exosomes are small extracellular vesicles that play important roles in intercellular communication and have potential therapeutic applications in regenerative medicine. Dermal mesenchymal stem cells (DMSCs) are a promising source of exosomes due to their regenerative and immunomodulatory properties. However, the molecular mechanisms regulating exosome secretion from DMSCs are not fully understood., Results: In this study, the role of peroxiredoxin II (Prx II) in regulating exosome secretion from DMSCs and the underlying molecular mechanisms were investigated. It was discovered that depletion of Prx II led to a significant reduction in exosome secretion from DMSCs and an increase in the number of intracellular multivesicular bodies (MVBs), which serve as precursors of exosomes. Mechanistically, Prx II regulates the ISGylation switch that controls MVB degradation and impairs exosome secretion. Specifically, Prx II depletion decreased JNK activity, reduced the expression of the transcription inhibitor Foxo1, and promoted miR-221 expression. Increased miR-221 expression inhibited the STAT signaling pathway, thus downregulating the expression of ISGylation-related genes involved in MVB degradation. Together, these results identify Prx II as a critical regulator of exosome secretion from DMSCs through the ISGylation signaling pathway., Conclusions: Our findings provide important insights into the molecular mechanisms regulating exosome secretion from DMSCs and highlight the critical role of Prx II in controlling the ISGylation switch that regulates DMSC-exosome secretion. This study has significant implications for developing new therapeutic strategies in regenerative medicine. Video Abstract., (© 2023. BioMed Central Ltd., part of Springer Nature.)
- Published
- 2023
- Full Text
- View/download PDF
18. Salt reduction behavior of adults in Anhui province in 2019: a cross-sectional survey of 3,378 participants.
- Author
-
Xing XY, Zhao Y, Sam NB, Xu JQ, Chen YJ, Xu W, Wang HD, Liu ZR, and Pan HF
- Subjects
- Humans, Adult, Adolescent, Young Adult, Middle Aged, Aged, Cross-Sectional Studies, China epidemiology, Risk Factors, Sodium Chloride, Dietary, Hypertension epidemiology
- Abstract
Objective: A high-sodium diet is an important risk factor for hypertension in the Chinese population, which can increase the risk of cardiovascular and cerebrovascular diseases. Although a large number of related studies have been carried out in Anhui province, clear, effective salt reduction interventions and policies that can be widely promoted have not yet been formed. This study sought to understand the prevalence and precise measures of salt reduction behavior, the variables affecting salt reduction behavior, and the reasons why salt reduction behavior was not practiced in Anhui Province, China., Methods: The total number of participants in the study was 3,378. Using a multi-stage stratified cluster random sampling method, residents between the ages of 18 and 69 years in 10 counties and districts were selected from March to October 2019. A survey questionnaire and physical measurements were given to each participant. The influencing factors of residents' salt reduction behavior were examined using a multi-factor unconditional logistic regression analysis. The chi-squared (χ
2 ) test was used to analyze the implementation of salt reduction behaviors among different age groups and gender, the factors influencing the implementation of salt reduction measures, and the reasons for not implementing salt reduction measures., Results: A history of hypertension was associated with salt reduction strategies ( P = 0.014). Patients with hypertension were more likely to adopt salt reduction behaviors than those without hypertension ( OR = 1.218, P = 0.040). The influence of eating out on the adoption of salt-reduction measures varied by age group (χ2 = 50.463, P < 0.001) and gender (χ2 = 81.348, P < 0.001)., Conclusion: In summary, residents of the Anhui Province are not very knowledgeable about salt reduction. Age, gender, education level, hypertension, and marital status are the main determinants. Our findings have significant implications for policymakers who want to devise salt reduction strategies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Xing, Zhao, Sam, Xu, Chen, Xu, Wang, Liu and Pan.)- Published
- 2023
- Full Text
- View/download PDF
19. [Urban-rural disparities of depression symptoms and its influencing factors among the elderly aged ≥65 years old in Anhui Province from 2019 to 2020].
- Author
-
Xing XY, Chen YJ, Xu XT, Wang HD, and Liu ZR
- Subjects
- Child, Aged, Humans, Female, Logistic Models, Risk Factors, Depression epidemiology, Schools
- Abstract
Objective: To analyze the difference in depression symptoms and influencing factors between urban and rural elderly people aged ≥65 years old in Anhui Province. Methods: Based on the data from a survey of 68 communities in Anhui Province that implemented the National Elderly Psychological Care Project from 2019 to 2020, the current status of depression symptoms in the elderly was evaluated using the Patient Health Questionnaire-9 (PHQ-9). The difference in the detection rate of depression symptoms between urban and rural elderly people with different characteristics was compared by using the χ
2 test. The logistic regression model was used to analyze the relevant factors of depression symptoms in urban and rural elderly people. Results: A total of 15 532 elderly people aged≥65 years old were included in the survey. The detection rate of depressive symptoms was 7.12%, which was higher in rural areas (9.08%) than in urban areas (6.48%). Logistic regression showed that chronic diseases were risk factors for depressive symptoms in elderly people from both urban and rural areas. Positive attitudes towards aging and good mental resilience were protective factors for depressive symptoms in elderly people. Having hobby ( OR =0.64, 95% CI : 0.45-0.91), good relationship with children ( OR =0.56, 95% CI : 0.41-0.76), good relationship with spouse ( OR =0.51, 95% CI : 0.37-0.71), and having at least 6 friends ( OR =0.48, 95% CI : 0.32-0.71) were the protective factors for depressive symptoms in urban elderly people. A good relationship with neighbors ( OR =0.58, 95% CI : 0.41-0.82) and having 1-2 friends ( OR =0.40, 95% CI : 0.25-0.64) were the protective factors for depressive symptoms in rural elderly people. Women ( OR =1.49, 95% CI : 1.06-2.10) and higher education level ( OR =1.81, 95% CI : 1.19-2.74, compared with illiterate/semi-illiterate in primary school; OR =2.94, 95% CI : 1.82-4.76, compared with illiterate/semi-illiterate in junior high school and above) were the risk factors for depressive symptoms in rural elderly people. Conclusion: There are differences between urban and rural areas in depressive symptoms among elderly people in Anhui Province. The detection rate of depression symptoms among rural elderly people is higher, and the influencing factors of depressive symptoms between urban and rural elderly people are also different, which should be treated specifically in the implementation of intervention measures.- Published
- 2023
- Full Text
- View/download PDF
20. Regulatory pathway underpinning the development of encephalitis after simian immunodeficiency virus infection in rhesus macaques (Macaca mulatta).
- Author
-
Kwon T, Xiang HY, Xing XY, Jiang P, Sun SY, Sun HN, and Han YH
- Subjects
- Humans, Animals, Macaca mulatta, Viral Load, HIV Infections, Simian Immunodeficiency Virus genetics, Simian Acquired Immunodeficiency Syndrome, Encephalitis
- Abstract
Background: Simian immunodeficiency virus (SIV) infection in rhesus macaques (Macaca mulatta) can lead to the development of SIV encephalitis (SIVE), which is closely related to human immunodeficiency virus (HIV)-induced dementia., Methods: This was done by analyzing SIV and SIVE encephalitis in infected M. mulatta hippocampus samples from two microarray data sets, identifying two groups of common differentially expressed genes and predicting associated protein interactions., Results: We found that eight genes-MX1, B2M, IFIT1, TYMP, STAT1, IFI44, ISG15, and IFI27-affected the negative regulation of biological processes, hepatitis C and Epstein-Barr viral infection, and the toll-like receptor signaling pathway, which mediate the development of encephalitis after SIV infection. In particular, STAT1 played a central role in the process by regulating biopathological changes during the development of SIVE., Conclusion: These findings provide a new theoretical basis for the treatment of encephalopathy after HIV infection by targeting STAT1., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
21. Induction of Targeted Differentiation of Dermal Mesenchymal Stem Cells Into Neural Lineage According to Peroxiredoxin II Expression.
- Author
-
Han YH, Xing XY, Lee DH, Mao YY, Jin MH, Sun HN, and Kwon T
- Subjects
- Peroxiredoxins genetics, Peroxiredoxins metabolism, Reactive Oxygen Species metabolism, Cell Differentiation genetics, RNA, Messenger genetics, Gene Regulatory Networks, RNA, Long Noncoding genetics, MicroRNAs genetics, Mesenchymal Stem Cells metabolism
- Abstract
Background/aim: To optimize the therapeutic potential of stem cells in stem cell therapy for neurological diseases, it is crucial to enhance the differentiation, migration, and neural network formation of stem cells, and to eliminate uncertain cell differentiation and proliferation factors. Several studies have shown that reactive oxygen species (ROS) are important factors in the regulation of neurogenesis, and Prx II (Peroxiredoxin II) is a gene that regulates ROS., Materials and Methods: As the entry point in this study to conduct a bioinformatics analysis of the sequencing results of Prx II
+/+ dermal mesenchymal stem cells (DMSCs) and Prx II-/- DMSCs. lncRNA/miRNA/mRNA networks were then constructed and preliminarily verified in RT-qPCR experiments., Results: In this study, a total of 11 hub genes (Gria1, Nrcam, Sox10, Snap25, Cntn2, Dlg2, Ngf, Ntrk3, Amph, Syt1, and Cd24a), eight miRNAs (miRNA-4661, miRNA-34a, miRNA-185, miRNA-34b-5p, miRNA-34c, miRNA-449a, miRNA-449b, miRNA-449c) and 12 lncRNAs (Dubr, Gas5, Gm20427, Gm26917, Gm42547, Gm8066, Kcnq1ot1, Malat1, Mir17hg, Neat1, Rian, and Tug1) were predicted in lncRNA/miRNA/mRNA network., Conclusion: The regulatory mechanism of Prx II in the differentiation of DMSCs into neurons through ROS was explored, and a theoretical basis was determined that can be applied in future research on nervous system diseases and the clinical applications of stem cells., (Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
22. Integrated transcriptome and metabolome analysis reveals molecular responses of spider to single and combined high temperature and drought stress.
- Author
-
Chen LJ, Li ZZ, Zhou XW, Xing XY, and Lv B
- Subjects
- Animals, Temperature, Droughts, Metabolome, Stress, Physiological, Transcriptome, Spiders genetics
- Abstract
High temperature and drought are abiotic stresses restricting many arthropods' survival and growth. Wolf spiders are poikilothermic arthropods that are vital in managing insects and pests. Nonetheless, investigating changes in spiders under temperature and drought stress are limited, especially at the molecular and gene expression levels. The study found that the combined effects of high temperature and drought stress significantly reduced survival rates and raised superoxide dismutase and malondialdehyde levels in the wolf spider Pardosa pseudoannulata. An integrated transcriptome and metabolome analysis showed that differentially expressed genes and metabolites were highly enriched in pathways involved in the proteolysis and oxidation-reduction process. The gene expression profiles displayed that heat shock protein (HSP) families (i.e., small heat shock protein, HSP70, HSP90, and HSP beta protein) were up-regulated under temperature and/or drought stresses. Additionally, a conjoint analysis revealed that under the combined stress, several important enzymes, including maltase-glucoamylase, glycerol-6-phosphate transporter, alanine-glyoxylate transaminase, and prostaglandin-H2 D-isomerase, were altered, affecting the metabolism of starch, sucrose, amino acids, and arachidonic acid. The protein interaction network further confirmed that under the combined stress, metabolic processes, peptide metabolic processes, and ATP generation from ADP were up-regulated, indicating that spiders could accelerate the metabolism of carbohydrates and proteins to combat stress and maintain homeostasis. Overall, this work showed that exposure to a combination of pressures might cause distinct defensive reactions in spiders and offered novel perspectives to research the molecular underpinnings of spider adaptation to a changing climate., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
23. [Clinical analysis of 5 cases of dermatomyositis complicated with macrophage activation syndrome].
- Author
-
Xing XY, Zhang JX, Zhu FY, Wang YF, Zhou XY, and Li YH
- Subjects
- Humans, Female, Middle Aged, Autoantibodies, Ferritins therapeutic use, Fibrinogen therapeutic use, Dermatomyositis complications, Macrophage Activation Syndrome etiology, Macrophage Activation Syndrome complications, Afibrinogenemia complications, Neutropenia, Thrombocytopenia complications, Hypertriglyceridemia complications
- Abstract
To investigate the clinical and immunological features of dermatomyositis (DM) complicated with macrophage activation syndrome (MAS). The demographic and clinical characteristics of five patients diagnosed with DM complicated with MAS hospitalized in the Department of Rheumatology and Immunology, Peking University People ' s Hospital from 2011 to 2021 were collected. The results of clinical manifestations, laboratory tests, immunological features, treatments and prognosis were analyzed and summarized. In this study, five female patients in Peking University People's Hospital with an average age of 63.8 (44.0-83.0) years and an average disease duration of 16.1 (1.5-48.0) months. All the patients had typical DM rash (such as heliotrope sign, V/shawl sign or Gottron's sign/papules). They all had muscle involvement (including myalgia or muscle weakness). Two patients had positive myositis-specific antibodies (MSAs), in which case 1 had anti-TIF1-γ antibody and case 5 had anti-NXP-2 antibody. Four patients had interstitial lung disease except case 3. All of the cases developed MAS in the active stage of DM. Common manifestations of MAS in these five patients included high-grade fever, cytopenia, decreased fibrinogen, elevated ferritin and increased soluble CD25. Case 1 presented with neutropenia (0.6×10
9 /L), thrombocytopenia (26.0×109 /L), hypofibrinogenemia (0.9 g/L), markedly elevated ferritin (26 331.0 μg/L), decreased NK cell activity. Case 2 had anaemia (hemoglobin 81.0 g/L), thrombocytopenia (55.0×109 /L), hypertriglyceridemia (4.7 mmol/L), hypofibrinogenemia (1.2 g/L), elevated ferritin (>100 000.0 μg/L), hemophagocytosis in bone marrow. Case 3 had anaemia (hemoglobin 88 g/L), decreased fibrinogen (1.9 g/L), increased ferritin (>27 759.0 μg/L), splenomegaly, hemophagocytosis in bone marrow. Case 4 suffered from neutropenia(0.3×109 /L), anaemia(hemoglobin 78 g/L), hypertriglyceridemia (4.2 mmol/L), hypofibrinogenemia (0.9 g/L), increased ferritin (>100 000.0 μg/L), and decreased NK cell activity. Case 5 presented anaemia (hemoglobin 60.0 g/L), thrombocytopenia (67.0×109 /L), hypertriglyceridemia (12.7 mmol/L), decreased fibrinogen (1.1 g/L), and elevated ferritin (>923.0 μg/L). All the patients were treated with methylprednisone pulse therapy (200-500 mg) combined with cyclosporine while case 5 received rituximab after methylprednisone pulses. In addition, case 3 also received the combination of mycophenolate mofetil. Case 1 was given etoposide while case 4 was treated with cyclophosphamide and repeated plasmapheresis at the same time. Moreover, intravenous immunoglobulin was added meantime apart from case 3. The condition of four patients improved significantly, nevertheless case 4 experienced recurred pulmonary symptoms and died of respiratory failure. As for complications about infection, case 2 had bacterial infection with high level procalcitonin (PCT) before MAS treatment and condition was improved after empiric antibacterial therapy. Case 3 had cytomegalovirus DNAemia before diagnosis of MAS and viral titer turned negative after ganciclovir therapy. After treatment of MAS, four patients developed cytomegalovirus DNAemia except case 3, in which case 5 was co-infected with bacteria. To sum, DM complicated with MAS is relatively rare, and its patients are of ten in life-threatening condition. Early detection, treatment and prevention of infection during treatment are critical to improve the prognosis.- Published
- 2022
24. Validation of the vault prediction model based on the sulcus-to-sulcus diameter and lens thickness: a 925-eye prospective study.
- Author
-
Zhu QJ, Xing XY, Zhu MH, Ma L, Yuan Y, and Song E
- Subjects
- Humans, Prospective Studies, Seasons, Ciliary Body
- Abstract
Background: To verify the accuracy and stability of the prediction formula based on the ciliary sulcus diameter and lens thickness and to analyse factors influencing the prediction results., Methods: In total, 925 eyes from 506 subjects were enrolled in this prospective study between July 1, 2020, and June 30, 2021. Subjects were divided into four seasons, each spanning three months. The target vault was set to be between 300 μm and 700 μm according the prediction formula. The actual vault was measured one month postoperatively. The Bland-Altman test, 95% confidence intervals (95% CI) and 95% limits of agreement (95% LoA) were used to evaluate the agreement between the predicted vault and the actual vault. Eyes with absolute prediction errors greater than 300 μm were further analysed., Results: The mean predicted vaults for the four seasons were 503 ± 99, 494 ± 96, 481 ± 92 and 502 ± 93 μm, while the mean actual vaults were 531 ± 189, 491 ± 179, 464 ± 179 and 529 ± 162 μm, respectively. The predicted and actual vaults of the overall subjects were 493 ± 95 and 500 ± 180 μm, respectively. Of the 925 eyes, 861 eyes (93.08%), 42 eyes (4.54%), and 22 eyes (2.38%) showed a normal vault, high vault, and low vault, respectively. Bland-Altman plots showed that the mean difference between the actual vault and predicted vault overall (± 95% LoA) was 6.43 ± 176.2 μm (-339 to 352 μm). Three UBM features may lead to large prediction errors (more than 300 μm): wide iris-ciliary angle (ICA), iris concavity and anteriorly positioned ciliary body., Conclusions: This study demonstrated the accuracy and stability of the prediction formula through the validation of a large sample size and a long time span. Wide ICA, iris concavity and anteriorly positioned ciliary body may have an effect on vault., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
25. [A cross-sectional study of prevalence of chronic kidney disease and related factors in adults in Anhui province].
- Author
-
Xu W, Xing XY, Xu JQ, Cao D, He Q, Dai D, Jia SC, Cheng QY, Lyu YL, Zhang L, Liang L, Xie GD, Chen YJ, Wang HD, and Liu ZR
- Subjects
- Adult, Male, Female, Humans, Adolescent, Cross-Sectional Studies, Prevalence, Hyperuricemia epidemiology, Renal Insufficiency, Chronic epidemiology, Hypertension epidemiology
- Abstract
Objective: To understand the prevalence of chronic kidney disease (CKD) and related factors in adults in Anhui province based on the data of Chinese Chronic Diseases and Nutrition Surveillance program (2018) in Anhui. Methods: Multi-stage stratified cluster random sampling was used to select participants aged ≥18 years. Moreover, questionnaire survey, body measurements and laboratory tests were conducted. The complex weighting method was used to estimate the prevalence of CKD in residents with different characteristics, and complex sampling data logistic regression model was used for multivariate analysis to identify related risk factors. Results: A total of 7 181 participants were included. The overall prevalence of CKD was 11.06% in adults in Anhui, and the prevalence was 12.49% in women and 9.59% in men ( P <0.05). The moderate, high and very high risk for CKD progression were 8.66%, 2.02% and 0.38%, respectively. Multivariate analysis showed that age ( OR =1.03, 95% CI : 1.00-1.05), BMI ( OR =1.05, 95% CI : 1.01-1.09), being woman ( OR =1.38,95% CI : 1.22-1.55), hypertension ( OR =2.50, 95% CI : 1.76-3.56), diabetes ( OR =2.28, 95% CI : 1.51-3.43), dyslipidemia ( OR =1.26, 95% CI : 1.11-1.43) and hyperuricemia ( OR =2.16, 95% CI : 1.68-2.78) were risk factors for CKD. Conclusion: The prevalence of CKD in adults in Anhui was relatively high and age, gender, BMI, hypertension, diabetes, dyslipidemia and hyperuricemia were found to be associated with the prevalence of CKD. To prevent CKD and its complications, attention should be paid to the management of related risk factors, including overweight and obesity, hypertension, diabetes, dyslipidemia and hyperuricemia.
- Published
- 2022
- Full Text
- View/download PDF
26. BgNet: Classification of benign and malignant tumors with MRI multi-plane attention learning.
- Author
-
Liu H, Jiao ML, Xing XY, Ou-Yang HQ, Yuan Y, Liu JF, Li Y, Wang CJ, Lang N, Qian YL, Jiang L, Yuan HS, and Wang XD
- Abstract
Objectives: To propose a deep learning-based classification framework, which can carry out patient-level benign and malignant tumors classification according to the patient's multi-plane images and clinical information., Methods: A total of 430 cases of spinal tumor, including axial and sagittal plane images by MRI, of which 297 cases for training (14072 images), and 133 cases for testing (6161 images) were included. Based on the bipartite graph and attention learning, this study proposed a multi-plane attention learning framework, BgNet, for benign and malignant tumor diagnosis. In a bipartite graph structure, the tumor area in each plane is used as the vertex of the graph, and the matching between different planes is used as the edge of the graph. The tumor areas from different plane images are spliced at the input layer. And based on the convolutional neural network ResNet and visual attention learning model Swin-Transformer, this study proposed a feature fusion model named ResNetST for combining both global and local information to extract the correlation features of multiple planes. The proposed BgNet consists of five modules including a multi-plane fusion module based on the bipartite graph, input layer fusion module, feature layer fusion module, decision layer fusion module, and output module. These modules are respectively used for multi-level fusion of patient multi-plane image data to realize the comprehensive diagnosis of benign and malignant tumors at the patient level., Results: The accuracy (ACC: 79.7%) of the proposed BgNet with multi-plane was higher than that with a single plane, and higher than or equal to the four doctors' ACC (D1: 70.7%, p=0.219; D2: 54.1%, p<0.005; D3: 79.7%, p=0.006; D4: 72.9%, p=0.178). Moreover, the diagnostic accuracy and speed of doctors can be further improved with the aid of BgNet, the ACC of D1, D2, D3, and D4 improved by 4.5%, 21.8%, 0.8%, and 3.8%, respectively., Conclusions: The proposed deep learning framework BgNet can classify benign and malignant tumors effectively, and can help doctors improve their diagnostic efficiency and accuracy. The code is available at https://github.com/research-med/BgNet., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Liu, Jiao, Xing, Ou-Yang, Yuan, Liu, Li, Wang, Lang, Qian, Jiang, Yuan and Wang.)
- Published
- 2022
- Full Text
- View/download PDF
27. Glycemic control and its influencing factors in type 2 diabetes patients in Anhui, China.
- Author
-
Xing XY, Wang XY, Fang X, Xu JQ, Chen YJ, Xu W, Wang HD, Liu ZR, and Tao SS
- Subjects
- Humans, Female, Adolescent, Young Adult, Adult, Middle Aged, Aged, Male, Glycated Hemoglobin, Glycemic Control, Obesity, Abdominal, China epidemiology, Obesity epidemiology, Diabetes Mellitus, Type 2 epidemiology
- Abstract
Objective: To investigate the status of glycemic control and analyze its influencing factors in patients with type 2 diabetes (T2D) in Anhui, China., Methods: 1,715 T2D patients aged 18-75 years old were selected from 4 counties or districts in Anhui Province in 2018, using a convenience sampling method. All patients have undergone a questionnaire survey, physical examination, and a glycosylated hemoglobin (HbA1c) test. According to the 2022 American Diabetes Association criteria, HbA1c was used to evaluate the glycemic control status of patients, and HbA1c < 7.0% was defined as good glycemic control. The influencing factors of glycemic control were analyzed by multivariate unconditional logistic regression., Results: The prevalence of good glycemic control among people with T2D in the Anhui Province was low (22.97%). On univariate analysis, gender, education level, occupation, region, smoking, drinking, waist circumference and disease duration (all P < 0.05) were significantly associated with glycemic control. The factors associated with pool glycemic control were female gender [OR = 0.67, 95%CI (0.52, 0.86), P = 0.001], higher level of education [OR = 0.47, 95%CI (0.27, 0.83), P = 0.001], living in rural areas [OR = 1.77, 95%CI (1.39, 2.26), P < 0.001], central obesity [OR = 1.58, 95%CI (1.19, 2.09), P = 0.001] and longer duration of disease [OR = 2.66, 95%CI (1.91, 3.69), P < 0.001]., Conclusions: The prevalence of good glycemic control in people with T2D in Anhui Province was relatively low, and gender, region, education level, central obesity and course of the disease were influencing factors. The publicity and education on the importance of glycemic control should be further strengthened in T2D patients, and targeted intervention measures should be carried out for risk groups., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Xing, Wang, Fang, Xu, Chen, Xu, Wang, Liu and Tao.)
- Published
- 2022
- Full Text
- View/download PDF
28. [Research progress of Shenling Baizhu San and predictive analysis on its quality markers].
- Author
-
Lu GY, Xing XY, Wang JY, Wang Y, Ma K, and Wang SJ
- Subjects
- Humans, Diarrhea drug therapy, Ginsenosides therapeutic use, Drugs, Chinese Herbal therapeutic use, Drugs, Chinese Herbal pharmacology, Colitis, Ulcerative drug therapy
- Abstract
Shenling Baizhu San is a classic prescription for replenishing Qi to invigorate the spleen and dispelling dampness to check diarrhea, which mainly treats the syndrome of spleen deficiency and heavy dampness. With the pharmacological effects of regulating immune system, improving lung function and gastrointestinal function, and resisting oxygen, tumor, and inflammation, Shenling Baizhu San is commonly used in modern clinical practice to treat chronic obstructive pulmonary disease, pulmonary fibrosis, bronchial asthma, irritable bowel syndrome, ulcerative colitis, chronic diarrhea, and diabetic, etc. This paper summarized the chemical constituents, pharmacological effects, and clinical application of Shenling Baizhu San in recent years, and predictively analyzed the quality markers of Shenling Baizhu San according to the "five principles" of Q-marker. The Q-markers of Shenling Baizhu San involved ginsenoside Rg_1, ginsenoside Re, ginsenoside Rb_1, pachymic acid, dehydrotumulosic acid, batatasin Ⅰ, batatasin Ⅲ, diosgenin, liensinine, neferine, luteolin, quercetin, glycerol trioleate, β-sitosterol, platycodin D, glycyrrhizic acid, glycyrrhetinic acid, liquiritin, pipecolinic acid, atractylenolide Ⅰ, atractylenolide Ⅲ, and bornyl acetate, which provided references for the quality control and follow-up research of Shenling Baizhu San.
- Published
- 2022
- Full Text
- View/download PDF
29. The detection rate and influencing factors of high-risk groups of cardiovascular disease in Anhui, China: A cross-sectional study of 99,821 residents.
- Author
-
Xing XY, Wang ZX, Cao YW, Wang XY, Zhang L, Chen YJ, Wang HD, Xu JQ, Niu MX, Liu ZR, and Tao SS
- Subjects
- Adult, Aged, China epidemiology, Cross-Sectional Studies, Humans, Male, Middle Aged, Obesity epidemiology, Obesity, Abdominal, Prevalence, Cardiovascular Diseases epidemiology
- Abstract
Objective: To investigate the detection rate and influencing factors of high-risk population of cardiovascular disease in Anhui province., Methods: From March 2017 to August 2019, the residents aged 35-75 years old were selected using the multi-stage stratified cluster sampling method in 8 counties and districts of Anhui Province, and questionnaire survey, anthropometric measurement, and collection of biological samples were carried out among them., Results: A total of 99,821 residents in Anhui Province were finally investigated, and among them 21,426 residents were detected to be high-risk groups of cardiovascular disease. The detection rate of high-risk groups was 21.46%. According to the high-risk types, the high-risk groups can be clustered. 74.57% of them had only one high-risk type, 22.57% of them had two high-risk types, and 2.86% had three or more high-risk types. The results of Generalized Linear Mixed Model (GLMM) showed that male, age ≥45 years old, not married, occupation as a farmer, annual family income <25,000 yuan, drinking, overweight and obesity, pre-central obesity and central obesity, snoring, feeling fatigued, sleepiness, and self-reported history of diabetes were more likely to be risk factors of cardiovascular disease (all P value < 0.05)., Conclusion: The detection rate of high-risk groups of cardiovascular disease in Anhui Province is relatively high. Individualized intervention measures as well as comprehensive prevention and control strategies should be adopted focusing on the distribution characteristics of risk factors of high-risk groups., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Xing, Wang, Cao, Wang, Zhang, Chen, Wang, Xu, Niu, Liu and Tao.)
- Published
- 2022
- Full Text
- View/download PDF
30. [The relationship between insulin resistance and risk of long-term mortality in people without diabetes: a 30-year follow-up of the Daqing Diabetes Study].
- Author
-
Hui YC, Wang JP, He SY, Xing XY, Wang X, Zhao F, Qian X, Li H, Gong QH, An YL, Chen YY, and Li GW
- Subjects
- Blood Glucose, Follow-Up Studies, Glucose Tolerance Test, Humans, Insulin, Diabetes Mellitus, Type 2, Insulin Resistance
- Abstract
Objective: To determine whether insulin resistance is associated with all-cause mortality in subjects without diabetes. Methods: A total of 505 participants without diabetes, 198 with normal glucose tolerance (NGT) and 307 with impaired glucose tolerance (IGT), were recruited from the Daqing Diabetes Study. The participants were followed up for 30 years. They were stratified into three groups (tertiles) according to baseline homeostasis model assessment of insulin resistance(HOMA-IR) levels, as the HOMA-IR 0, the HOMA-IR 1 and the HOMA-IR 2 groups, to assess the predictive effect of insulin resistance on risk of all-cause mortality. Results: During the 30-year follow-up, 52, 56 and 78 participants died across the three HOMA-IR groups, respectively. The corresponding mortality per 1 000 person-years (95 %CI ) were 12.12 (9.56-15.01), 13.10 (10.46-16.03) and 19.91 (16.73-23.15), respectively. Participants in the HOMA-IR 2 group had a significantly higher risk of death than those in the HOMA-IR 0 group after adjustment of age, sex and smoking status ( HR =1.97,95 %CI 1.38-2.81, P< 0.001). Cox analyses showed that a one standard deviation increase in HOMA-IR was associated with a 22% increase in the mortality after adjustment of potential confounders ( HR =1.22, 95 %CI 1.08-1.39, P= 0.002). Conclusions: Insulin resistance is associated with increased risk of all-cause death in Chinese people without diabetes, suggesting that improving insulin resistance could be beneficial for people without diabetic in reducing risk of long-term all-cause mortality.
- Published
- 2022
- Full Text
- View/download PDF
31. Preparation and Properties of Thin-Film Composite Forward Osmosis Membranes Supported by Cellulose Triacetate Porous Substrate via a Nonsolvent-Thermally Induced Phase Separation Process.
- Author
-
Han JC, Xing XY, Wang J, and Wu QY
- Abstract
A porous substrate plays an important role in constructing a thin-film composite forward osmosis (TFC-FO) membrane. To date, the morphology and performance of TFC-FO membranes are greatly limited by porous substrates, which are commonly fabricated by non-solvent induced phase separation (NIPS) or thermally induced phase separation (TIPS) processes. Herein, a novel TFC-FO membrane has been successfully fabricated by using cellulose triacetate (CTA) porous substrates, which are prepared using a nonsolvent-thermally induced phase separation (N-TIPS) process. The pore structure, permeability, and mechanical properties of CTA porous substrate are carefully investigated via N-TIPS process (CTA
N-TIPS ). As compared with those via NIPS and TIPS processes, the CTAN-TIPS substrate shows a smooth surface and a cross section combining interconnected pores and finger-like macropores, resulting in the largest water flux and best mechanical property. After interfacial polymerization, the obtained TFC-FO membranes are characterized in terms of their morphology and intrinsic transport properties. It is found that the TFC-FO membrane supported by CTAN-TIPS substrate presents a thin polyamide film full of nodular and worm-like structure, which endows the FO membrane with high water permeability and selectivity. Moreover, the TFC-FO membrane supported by CTAN-TIPS substrate displays a low internal concentration polarization effect. This work proposes a new insight into preparing TFC-FO membrane with good overall performance.- Published
- 2022
- Full Text
- View/download PDF
32. Transcriptome analysis of genes associated with autolysis of Coprinus comatus.
- Author
-
Guo HB, Zhang ZF, Wang JQ, Wang SY, Yang JK, Xing XY, Qi XJ, and Yu XD
- Subjects
- China, Coprinus growth & development, Coprinus metabolism, Gene Ontology, High-Throughput Nucleotide Sequencing, Metabolic Networks and Pathways, Real-Time Polymerase Chain Reaction, Coprinus genetics, Food, Fruiting Bodies, Fungal genetics, Fruiting Bodies, Fungal physiology, Gene Expression Profiling methods, Genes, Fungal genetics, Transcriptome genetics
- Abstract
Coprinus comatus, widely known as "Jituigu", is an important commodity and food in China. The yield of C. comatus, however, is substantially reduced by the autolysis of the fruiting bodies after harvest. To gain insight into the molecular mechanism underlying this autolysis, we divided the growth of C. comatus fruiting bodies into four stages: infant stage (I), mature stage (M), discolored stage (D), and autolysis stage (A). We then subjected these stages to de novo transcriptomic analysis using high-throughput Illumina sequencing. A total of 12,946 unigenes were annotated and analyzed with the Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG). We analyzed the differentially expressed genes (DEGs) between stages I and M, M and D, and D and A. Because the changes from M to D are thought to be related to autolysis, we focused on the DEGs between these two stages. We found that the pathways related to metabolic activity began to vary in the transition from M to D, including pathways named as autophagy-yeast, peroxisome, and starch and sucrose metabolism. This study also speculates the possible process of the autolysis of Coprinus comatus. In addition, 20 genes of interest were analyzed by quantitative real-time PCR to verify their expression profiles at the four developmental stages. This study, which is the first to describe the transcriptome of C. comatus, provides a foundation for future studies concerning the molecular basis of the autolysis of its fruiting bodies., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
33. [Clinical and immunological characteristics of patients with anti-synthetase syndrome complicated with cardiac involvement].
- Author
-
Luo L, Xing XY, Xiao YS, Chen KY, Zhu FY, Zhang XW, and Li YH
- Subjects
- Adult, Aged, Antibodies, Antinuclear, Autoantibodies, Blood Sedimentation, C-Reactive Protein, Humans, Immunoglobulin G, L-Lactate Dehydrogenase, Middle Aged, Myositis complications, Heart Diseases complications, Myositis diagnosis
- Abstract
Objective: To investigate the clinical and immunological features of cardiac involvement in patients with anti-synthetase syndrome (ASS)., Methods: In the study, 96 patients diagnosed with ASS hospitalized in the Department of Rheumatology and Immunology, Peking University People's Hospital from April 2003 to November 2020 were included. The patients were divided into two groups according to whether they were accompanied with cardiac involvement. Demographic features, clinical characteristics (Gottron's sign/papules, muscle damage, etc .), comorbidities, laboratory indices (creatine kinase, inflammatory indicators, immunoglobulin, complement, lymphocyte subset, autoantibodies, etc .) were collected and the differences between the two groups were analyzed statistically., Results: The prevalence of cardiac involvement in the patients with ASS was 25.0% (24/96). The ASS patients complicated with cardiac involvement presented with elevated cardiac troponin I (cTnI, 75.0%, 18/24), pericardial effusion (33.3%, 8/24), reduction of left ventricular function (33.3%, 8/24) and valves regurgitation (33.3%, 8/24). The age of onset of the patients with cardiac involvement was older than that of the patients without cardiac involvement [(54.58±10.58) years vs . (48.47±13.22) years, P =0.043). Arthritis was observed less frequently in the patients with cardiac involvement than those without cardiac involvement (37.5% vs . 61.1%, P =0.044). In addition, rapidly progressive interstitial lung disease (54.2% vs . 30.6%, P =0.037) was observed more frequently in the patients with cardiac involvement than those without cardiac involvement. As compared with the ASS patients without cardiac involvement, C-reactive protein (CRP) [(13.55 (8.96, 38.35) mg/L vs. 4.60 (1.37, 17.40) mg/L, P =0.001], and lactate dehydrogenase (LDH) [408.0 (255.0, 587.0) U/L vs . 259.5 (189.8, 393.8) U/L, P =0.007] were significantly higher in the patients with cardiac involvement. Anti-Ro-52 antibody was detected more commonly in the ASS patients with cardiac involvement compared with the patients without cardiac involvement (91.7% vs . 69.4%, P =0.029). No significant differences were found in the comorbidities, alanine transaminase (ALT), aspartate transaminase (AST), creatine kinase (CK), erythrocyte sedimentation rate (ESR), ferritin (Fer), immunoglobulin G (IgG), complement 3 (C3), complement 4 (C4), lymphocyte subset between the two groups., Conclusion: Cardiac involvement is common in ASS, mainly manifested as myocardial damage. It is necessary to be aware of cardiac complications in patients with elevated CRP, elevated LDH and positive anti-Ro-52 antibody.
- Published
- 2021
34. [Clinical and immunological characteristics of 88 cases of overlap myositis].
- Author
-
Xiao YS, Zhu FY, Luo L, Xing XY, Li YH, Zhang XW, and Shen DH
- Subjects
- Autoantibodies, Female, Humans, Retrospective Studies, Dermatomyositis epidemiology, Myositis epidemiology, Raynaud Disease
- Abstract
Objective: To investigate the clinical and immunological characteristics of overlap myositis (OM) patients., Methods: The data of 368 patients with idiopathic inflammatory myopathies (IIMs) admitted to Peking University People's Hospital from January 2004 to August 2020 were analyzed retrospectively, including demographic characteristics, clinical characteristics (including fever, Gottron' s sign/papules, Heliotrope rash, V-sign, Shawl sign, Mechanic' s hands, skin ulceration, periungual erythema, subcutaneous calcinosis, dysphagia, myalgia, myasthenia, arthritis, Raynaud' s phenomenon, interstitial lung disease, pulmonary hypertension and myocardial involvement), laboratory characteristics, immunological characteristics [including antinuclear antibodies, rheumatoid factors, myositis-associated autoantibodies (MAAs) and myositis-specific autoantibodies (MSAs)] and survival. The clinical and immunological characteristics and prognostic differences of OM and non-OM were compared. The Kaplan-Meier and Log Rank methods were used to analyze the survival., Results: A total of 368 patients were included. 23.9% (88/368) of IIMs patients were OM patients. Among the 88 OM patients, 85.2% (75/88) of them were female, and the median interval between disease onset and diagnosis was 13.5 months. The incidence of overlapped connective tissue diseases in the OM patients was dermatomyositis (DM) in 60.2%, polymyositis (PM) in 3.4%, immune-mediated necrotizing myopathy (IMNM) in 2.3% and anti-synthetase syndrome (ASS) in 34.1%. Compared with the non-OM patients, the proportion of the females in the OM patients was higher (85.2% vs. 72.1%, P =0.016), the OM patients had longer disease duration [13.5(4.5, 48.0) months vs . 4.0(2.0, 12.0) months, P < 0.001]. As for clinical characteristics, compared with the non-OM patients, the incidence of V-sign (25.0% vs . 44.6%, P =0.001) and periungual erythema (8.0% vs . 19.6%, P =0.013) were lower; the incidence of Raynaud's phenomenon (14.8% vs . 1.8%, P < 0.001), interstitial pneumonia (88.6% vs . 72.1%, P =0.001), pulmonary hypertension (22.7% vs . 7.5%, P < 0.001) and myocardial involvement (18.2% vs . 9.3%, P =0.033) were higher. As for immunological characteristics, compared with the non-OM patients, the incidence of elevated aspartate aminotransferase (AST) (31.8% vs . 45.0%, P =0.035) was lower and elevated C-reactive protein (CRP) (58.0% vs . 44.6%, P =0.037) was higher; the positive rates of antinuclear antibodies (ANA) (85.1% vs . 63.4%, P =0.001) and rheumatoid factors (RF) (40.2% vs . 17.8%, P < 0.001) and anti-Ro-52 (71.6% vs. 56.1%, P =0.038) in serum were higher. There was no significant difference in the survival between the OM patients and non-OM patients., Conclusion: Pulmonary hypertension and myocardial involvement were frequently observed in OM.
- Published
- 2021
35. [The Risk Factors of Thrombosis in Patients with Philadelphia Chromosome-negative Myeloproliferative Neoplasms].
- Author
-
Zhao YF, Lin SZ, Bai X, Xing XY, Tao HF, and Su YZ
- Subjects
- Humans, Philadelphia Chromosome, Risk Factors, Myeloproliferative Disorders genetics, Neoplasms, Thrombosis
- Abstract
Objective: To investigate the overview of thrombosis in myeloproliferative neoplasms(MPN) patients, and to explore the risk factors of thrombosis at diagnosis and during follow-up., Methods: The clinical data of 388 MPN patients treated in our hospital were collected. The patients were followed up by outpatient and phone. The risk factors of thrombosis were analyzed by statistical methods., Results: Among 388 MPN patients, 161 patients (41.49%) showed thromboses at diagnosis or during follow-up. Among them, 92.55% were arterial thromboses, 146 cases (96.27%) were complicated with thromboses at diagnosis, and 36 cases (11.46%) showed newly thromboses or progression of previous thromboses among the 314 received full follow-up patients. Age (P<0.001, HR:1.033, 95%CI:1.016-1.051), JAK2V617F mutation (P=0.037, HR:1.72, 95%CI: 1.033-2.862), hypertension (P<0.001, HR:2.639, 95%CI:1.659-4.197) and hyperlipidemia (P<0.001, HR:2.659, 95%CI:1.626-4.347) were the independent risk factors affecting thrombosis at diagnosis of the patients. During the follow-up, age (P=0.016, HR:1.032, 95%CI: 1.006-1.059) and previous thrombosis history (P=0.019, HR:2.194, 95%CI: 1.135-4.242) were the independent risk factors affecting the progression of thrombosis at different sites or on the basis of the previous thrombosis in the patients., Conclusion: Patients with advanced age, JAK2V617F mutation or complicated with hypertension and hyperlipidemia shows a higher risk of thrombosis at diagnosis, while the patients with advanced age or previous thrombosis history shows a higher risk of progression of thrombosis during the follow-up.
- Published
- 2021
- Full Text
- View/download PDF
36. Clinical Significance of Preoperative CT and MR Imaging Findings in the Prediction of Postoperative Recurrence of Spinal Giant Cell Tumor of Bone.
- Author
-
Wang QZ, Zhang EL, Xing XY, Su MY, and Lang N
- Subjects
- Bone Neoplasms pathology, Female, Giant Cell Tumor of Bone pathology, Humans, Male, Postoperative Period, Predictive Value of Tests, Preoperative Period, Retrospective Studies, Bone Neoplasms diagnostic imaging, Bone Neoplasms surgery, Giant Cell Tumor of Bone diagnostic imaging, Giant Cell Tumor of Bone surgery, Magnetic Resonance Imaging, Neoplasm Recurrence, Local pathology, Tomography, X-Ray Computed
- Abstract
Objectives: To explore the predictive value of preoperative imaging in patients with spinal giant cell tumor of bone (GCTB) for postoperative recurrence and risk stratification., Methods: Clinical data for 62 cases of spinal GCTB diagnosed and treated at our hospital from 2008 to 2018 were identified. All patients were followed up for more than 2 years according to the clinical guidelines after surgery. Medical history data including baseline demographic and clinical characteristics, computed tomography (CT) and magnetic resonance imaging (MRI) findings of recurrent and non-recurrent patients were compared. Two musculoskeletal radiologists read the images and were blinded to the clinical data. The imaging features associated with postoperative recurrence were analyzed by multivariate logistic regression, and receiver operating characteristic (ROC) curve analysis was performed to determine the optimal cutoff value of the largest lesion diameter predicting recurrence after surgery., Results: According to whether the disease recurred within the follow-up period, patients were divided into the recurrence group and the non-recurrence group. Of 62 patients (29 males and 33 females), 17 had recurrence and 45 did not. The recurrence rate was 27.4%. The mean follow-up time was 73.66 (± 32.92) months. The three major treatments were total en bloc spondylectomy (n = 26), intralesional spondylectomy (n = 20), and curettage(n = 16). A total of 16 CT and MRI features were analyzed. A univariate analysis showed no significant difference in age, sex, treatment, multi-vertebral body involvement, location, boundary, expansile mass, residual bone crest, paravertebral soft tissue mass, CT value, and MRI signal on T1-weighted imaging (WI), T2-WI, and T2-WI fat suppression (FS) sequences (P > 0.05). The largest lesion diameter [(4.68 ± 1.79) vs (5.92 ± 2.17) cm, t = 2.287, P = 0.026] and the vertebral compression fracture (51% vs 82%, χ
2 = 5.005, P = 0.025) were significantly different between the non-recurrence and recurrence groups. Logistic regression analysis showed that both largest lesion diameter (odds ratio [OR], 1.584; 95% confidence interval [CI], 1.108-2.264; P = 0.012) and compression fracture (OR, 8.073; 95%CI, 1.481-11.003; P = 0.016) were independent predictors of postoperative recurrence. When we set the cutoff value for the largest lesion diameter at 4.2 cm, the sensitivity and specificity for distinguishing the recurrence and non-recurrence of GCTB were 94.1% and 42.2%, respectively, and the area under the curve (AUC) was 0.671. The combined model achieved a sensitivity, specificity and accuracy of 47.1%, 97.8% and 83.9%, respectively., Conclusions: In spinal GCTB, maximum lesion diameter and the vertebral compression fracture are associated with tumor recurrence after surgery, which may provide helpful information for planning personalized treatment., (© 2021 The Authors. Orthopaedic Surgery published by Chinese Orthopaedic Association and John Wiley & Sons Australia, Ltd.)- Published
- 2021
- Full Text
- View/download PDF
37. [Analysis of risk factors for hyperkalemia in hemodialysis patients and establishment and verification of risk assessment model of hyperkalemia].
- Author
-
Xing XY, Yao L, Li YB, Zhang FX, Li P, Qiao YJ, Liang XH, Wang P, and Liu ZS
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, ROC Curve, Renal Dialysis, Retrospective Studies, Risk Assessment, Risk Factors, Hyperkalemia epidemiology
- Abstract
Objective: To explore risk factors for hyperkalemia in hemodialysis (HD) patients, and establish and verify a risk assessment model of hyperkalemia in HD patients. Methods: The clinical data of HD patients who were admitted to the Department of Nephrology of the First Affiliated Hospital of Zhengzhou University between April 2020 and January 2021 were retrospectively collected and divided into training dataset and validation dataset by using the conversion-random number generator. In the training dataset, multivariate logistic regression analysis was used to screen the risk factors for hyperkalemia in HD patients and the factors were scored to establish the risk assessment model. The validation dataset was substituted into the model and the receiver operating characteristic (ROC) curve was drawn and the area under the curve (AUC) was calculated to verify the effectiveness of the risk prediction model in predicting hyperkalemia. Results: A total of 502 HD patients were enrolled and further divided into training dataset ( n =372) and validation dataset ( n =130). There were 268 males and 234 females, with a mean age of (54±13) years. Multivariate logistic regression analysis showed that metabolic acidosis, high potassium diet, history of hyperkalemia, the change of electrocardiogram (ECG), disfunction of vascular access and time interval from last dialysis were risk factors for causing hyperkalemia in patients undergoing HD. Risk assessment model was established based on these risk factors. The AUC of the ROC curve was 0.799. Using 5 as the cut-off value, the sensitivity and specificity for predicting hyperkalemia events was 61.4% and 86.3%, respectively. Conclusion: The current study preliminarily established a risk assessment model for hyperkalemia in HD patients, which can help clinicians manage the potassium level of HD patients.
- Published
- 2021
- Full Text
- View/download PDF
38. Warming Shapes nirS- and nosZ -Type Denitrifier Communities and Stimulates N 2 O Emission in Acidic Paddy Soil.
- Author
-
Xing XY, Tang YF, Xu HF, Qin HL, Liu Y, Zhang WZ, Chen AL, and Zhu BL
- Subjects
- Global Warming, Hydrogen-Ion Concentration, Nitrification, Oryza, Soil chemistry, Temperature, Air Pollutants analysis, Denitrification, Nitrous Oxide analysis, Soil Microbiology, Soil Pollutants analysis
- Abstract
Warming strongly stimulates soil nitrous oxide (N
2 O) emission, contributing to the global warming trend. Submerged paddy soils exhibit huge N2 O emission potential; however, the N2 O emission pathway and underlying mechanisms for warming are not clearly understood. We conducted an incubation experiment using15 N to investigate the dynamics of N2 O emission at controlled temperatures (5, 15, 25, and 35°C) in 125% water-filled pore space. The community structures of nitrifiers and denitrifiers were determined via high-throughput sequencing of functional genes. Our results showed that elevated temperature sharply enhanced soil N2 O emission from submerged paddy soil. Denitrification was the main contributor, accounting for more than 90% of total N2 O emission at all treatment temperatures. N2 O flux was coordinatively regulated by nirK- , nirS- , and nosZ -containing denitrifiers but not ammonia-oxidizing archaea or ammonia-oxidizing bacteria. The nirS -containing denitrifiers were more sensitive to temperature shifts, especially at a lower temperature range (5 to 25°C), and showed a stronger correlation with N2 O flux than that of nirK -containing denitrifiers. In contrast, nosZ -containing denitrifiers exhibited substantial variation at higher temperatures (15 to 35°C), thereby playing an important role in N2 O consumption. Certain taxa of nirS - and nosZ -containing denitrifiers regulated N2 O flux, including nirS -containing denitrifiers affiliated with Rhodanobacter and Cupriavidus as well as nosZ -containing denitrifiers affiliated with Azoarcus and Azospirillum . Together, these findings suggest that elevated temperature can significantly increase N2 O emission from denitrification in submerged paddy soils by shifting the overall community structures and enriching some indigenous taxa of nirS - and nosZ -containing denitrifiers. IMPORTANCE The interdependence between global warming and greenhouse gas N2 O has always been the hot spot. However, information on factors contributing to N2 O and temperature-dependent community structure changes is scarce. This study demonstrated high-temperature-induced N2 O emission from submerged paddy soils, mainly via stimulating denitrification. Further, we speculate that key functional denitrifiers drive N2 O emission. This study showed that denitrifiers were more sensitive to temperature rise than nitrifiers, and the temperature sensitivity differed among denitrifier communities. N2 O-consuming denitrifiers ( nosZ -containing denitrifiers) were more sensitive at a higher temperature range than N2 O-producing denitrifiers ( nirS -containing denitrifiers). This study's findings help predict N2 O fluxes under different degrees of warming and develop strategies to mitigate N2 O emissions from paddy fields based on microbial community regulation.- Published
- 2021
- Full Text
- View/download PDF
39. [Estimation of dietary salt intake in adult residents in Anhui province, 2019].
- Author
-
Xu W, Xu JQ, Dai D, Zhu JJ, He Q, Xing XY, Chen YJ, and Liu ZR
- Subjects
- Adult, Blood Pressure, China, Cross-Sectional Studies, Female, Humans, Male, Waist Circumference, Hypertension epidemiology, Sodium Chloride, Dietary
- Abstract
Objective: Based on the data of the baseline survey of hypertension and sodium intake monitoring in Anhui province in 2019, the salt intake in adult residents was estimated. Methods: Multi-stage stratified cluster random sampling was used to select participants aged 18-69 years, questionnaire survey and related measurements were conducted. Salt intake in participants with different characteristics were estimated with complex sample and linearization of Taylor series based on design and the correlation between salt intake and blood pressure, waist circumference and BMI were tested by linear regression. Results: A total of 1 500 participants were included. The overall salt intake was 9.14 g/d, which was 9.84 g/d in men and 8.47 g/d in women ( P <0.05). The differences in salt intake across different subgroups were significant ( P <0.05). Univariate linear regression analysis showed that salt intake was positively correlated with SBP, DBP, waist circumference and BMI ( P <0.05), while multivariate linear regression analysis (adjusted for other factors) only showed a positive correlation between salt intake and BMI ( β =0.053,95% CI : 0.028-0.078, P <0.05). Conclusion: The dietary salt intake in adult residents in Anhui was higher than WHO recommendation, suggesting that public health education need to be taken to reduce salt intake.
- Published
- 2021
- Full Text
- View/download PDF
40. [Temporal trend of probability of premature death caused by four major non-communicable diseases in Anhui province, 2014 to 2018, and the potential of achieving healthy goals].
- Author
-
Xing XY, He Q, Cheng Q, Li R, Chen Y, and Liu Z
- Subjects
- China epidemiology, Female, Goals, Humans, Male, Risk Factors, Mortality, Premature, Noncommunicable Diseases prevention & control
- Abstract
Objective: To investigate the current status and temporal trend of probability of premature death, caused by four major non-communicable diseases (NCDs) in Anhui province, from 2014 to 2018, and to explore the possibility of achieving the goal of "Healthy China 2030". Methods: Data of death were collected from the "Information System for Death Cause Register and Management". The mortality, standardized mortality, premature mortality rate and annual percentage change (APC) were calculated. Based on the reduction target of "Healthy China 2030"(-30%) and "Healthy Anhui 2030"(-25%) for premature death probability of four major NCDs, the possibility of achieving the target was evaluated. Results: From 2014 to 2018, the proportion of four major NCDs deaths to total deaths increased from 82.76% to 84.77%, showing an upward trend(APC=0.50%). The standardized mortality rate of four major NCDs in the population aged 30-69 years was decreasing annually (APC=-1.78%). The premature death probability of four major NCDs decreased from 15.53% to 14.43% with a downward trend. The decline rate of men (APC=-1.80%) was slightly lower than that of women (APC = -2.00%). The decline rate of urban (APC=-1.57%) and rural (APC=-1.99%), Northern Anhui (APC=-2.32%) and Central Anhui (APC=-1.81%) areas were all showed a downward trend. The premature death probability in the same year was higher in men, rural areas and northern Anhui areas. The decrease of chronic respiratory diseases was the greatest (APC=-9.19%), followed by cancer (APC=-1.77%), but increased in diabetes for men (APC=-2.90%). According to the average growth rate of premature death probability from 2014 to 2018, it was predicted that the goal of healthy Anhui would be achieved by 2030. However, to achieve the goal of healthy China, the rate of decline needs to be increased by 26.40% on the existing basis. Comparing to the proportion, the descent speed needs to be increased between different genders, urban and rural areas and regions. Conclusions: The premature death probability of four major NCDs in Anhui province has a continuous downward trend. Men, rural residents and residents in northern Anhui province are the key population. We should carry out intervention against the main risk factors of NCDs, reduce the premature mortality rate of NCDs, and strive to achieve the goal of "Healthy China 2030".
- Published
- 2021
- Full Text
- View/download PDF
41. [Clinical and immunological characteristics of myositis complicated with thromboembolism].
- Author
-
Zhu FY, Xing XY, Tang XF, Li YM, Shao M, Zhang XW, Li YH, Sun XL, and He J
- Subjects
- Adult, Autoantibodies, Female, Humans, Male, Middle Aged, Retrospective Studies, Dermatomyositis, Lung Diseases, Interstitial, Myositis complications, Thromboembolism
- Abstract
Objective: To investigate and analyse the clinical and immunological features of patients with myositis complicated with thromboembolism., Methods: We identified a cohort of 390 myositis patients diagnosed with myositis admitted to People's Hospital of Peking University from 2003 to 2019. The patients were retrospectively enrolled in this investigation. According to the outcome of the color Doppler ultrasound, CT pulmonary angiography, pulmonary ventilation and perfusion scan patients were divided into myositis with and without thromboembolism group. Demographic, clinical (heliotrope rash, Gottron's sign/papules, periungual erythema, skin ulceration, subcutaneous calcinosis, Mechanic's hands, myalgia, interstitial lung disease, pulmonary arterial hypertension), laboratory, immunological [anti-autoantibodies including melanoma differentiation associated gene 5 (anti-MDA5), anti-Mi-2, anti-transcription intermediary factor-1γ (anti-TIF-1γ, anti-nuclear matrix protein 2 (anti-NXP2), anti-small ubiquitin-like modifier activating enzyme (anti-SAE), anti-synthetase], imaging and therapeutic status data of the patients at the diagnosis of myositis with and without thromboembolism were collected and the differences in these data were analyzed. Logistic regressive analysis was used to identify the risk factors of thromboembolism., Results: In the retrospective study, 390 myositis patients were investigated. The mean age of onset was (49.6±13.4) years, male to female ratio was 0.31 :1. Thromboembolism was identified in 4.62% (18/390) of the myositis patients, which was lower than the published reports. Out of 18 patients with thromboembolism, 55.6% (10/18) of them were deep venous thrombosis, followed by cerebral infarction (22.2%, 4/18), pulmonary embolism (11.1%, 2/18), renal artery embolism (5.6%, 1/18) and embolism of upper extremity (5.6%, 1/18). Fifty percent of thromboembolism events occurred 6 months after the diagnosis of myositis, 38.9% of thromboembolism events occurred 6 months within the diagnosis of myositis, 11.1% of thromboembolism events occurred 6 months before the diagnosis of myositis. As compared with the myositis patients without thromboembolism, the myositis patients complicated with thromboembolism were older [(58.3±11.7) years vs . (49.3±13.4) years, P =0.006]. C-reaction protein (CRP) (12.2 mg/L vs . 4.1 mg/L, P < 0.001), ferritin (20 085.5 μg/L vs . 216.6 μg/L, P < 0.001) and D-dimer (529.0 μg/L vs . 268.0 μg/L, P =0.002) were significantly higher in thromboembolism group. Diabetes (44.4% vs . 16.4%, P =0.006), coronary heart disease (22.2% vs . 3.0%, P =0.003) and surgery (16.7% vs . 3.5%, P =0.032) were observed more common in thromboembolism group than those without thromboembolism. Activated partial thromboplastin time (APTT) (26.9 s vs . 28.7 s, P =0.049) and albumin (32.4 g/L vs . 36.5 g/L, P =0.002) was lower in thromboembolism group. The risk factors of thromboembolism in the myositis patients were low level of albumin ( OR =0.831, 95% CI : 0.736-0.939, P =0.003), diabetes ( OR =4.468, 95% CI : 1.382-14.448, P =0.012), and coronary heart disease ( OR =22.079, 95% CI : 3.589-135.837, P =0.001) were independent significant risk factors for thromboembolism in the patients with myositis. There was no significant difference in clinical manifestations, myositis-specific antibodies or myositis-associated antibodies between the two groups., Conclusion: Thromboembolism is a complication of myositis. Lower levels of albumin, diabetes, and coronary heart disease might be risk factors of thromboembolism in myositis patients.
- Published
- 2020
42. [Effect of moxibustion on clinical symptoms, peripheral inflammatory indexes and T lymphocyte subsets in COVID-19 patients].
- Author
-
Liu L, Xing XY, He DC, Yang WC, Zhang MY, Wu W, Ding XJ, Yu Q, Huang HS, Sun XB, Zhang Y, and Yang JS
- Subjects
- Acupuncture Points, C-Reactive Protein analysis, Humans, Interleukin-6 blood, Leukocyte Count, COVID-19 therapy, Inflammation therapy, Moxibustion, T-Lymphocyte Subsets cytology
- Abstract
Objective: To explore the therapeutic effect and the mechanism of the adjuvant treatment with moxibustion on coronavirus disease 2019 (COVID-19)., Methods: A total of 95 patients with COVID-19 were randomly divided into a moxibustion group (45 cases) and a basic treatment group (50 cases). The routine treatment of western medicine was applied in the patients of both groups. In the moxibustion group, on the base of the treatment of western medicine, moxibustion was applied to Dazhui (GV 14), Feishu (BL 13), Qihai (CV 6) and Zusanli (ST 36), once daily and consecutively for 14 days. At the end of treatment courses, clinical symptom scores for cough, asthmatic breathing, chest oppression and short breath, as well as their remission rates were compared between the two groups before and after treatment. Before and after treatment, the white blood cell (WBC) count, the levels of c-reactive protein (CRP) and interleukin-6 (IL-6) and the absolute number of T lymphocyte subsets, i.e. , and of the peripheral blood were compared in the patients between the two groups. The principal component analysis was adopted to analyze the common data extracted from the above 10 clinical indexes variables and comprehensively evaluate the differences in the therapeutic effect of two regimens., Results: The clinical symptom scores were all decreased after treatment in both of the moxibustion group and the basic treatment group as compared with those before treatment ( P <0.05). After treatment, the clinical symptom scores of cough, chest oppression and asthmatic breathing in the moxibustion group were lower significantly than those in the basic treatment group ( P <0.05) and the remission rates of cough, chest oppression and asthmatic breathing were higher than the basic treatment group ( P <0.05). After treatment, WBC count was increased as compared with that before treatment in either group ( P <0.05) and the levels of CRP and IL-6 in the moxibustion group were reduced as compared with those before treatment ( P <0.05). The reducing range of IL-6 level in the moxibustion group was larger than the basic treatment group ( P <0.05). After treatment, the absolute number of , and T lymphocytes was increased as compared with that before treatment in the moxibustion group ( P <0.05), and its increase range was larger than the basic treatment group ( P <0.05). The difference value was 33.38 for the score of comprehensive evaluation before and after treatment in the moxbustion group, higher obviously than 8.91 in the basic treatment group., Conclusion: On the base of the routine treatment with western medicine, moxibustion therapy supplemented relieves the clinical symptoms, reduces the levels of inflammatory indexes, i.e. IL-6 and CRP as well as improves the absolute number of peripheral T lymphocyte subsets. The clinical therapeutic effect of such regimen with moxibustion supplemented is significantly better than the simple routine treatment of western medicine.
- Published
- 2020
- Full Text
- View/download PDF
43. [Clinical Analysis of 269 Ph Chromosome-Negative Myeloproliferative Neoplasms Patients Stratified by Age].
- Author
-
Tao HF, Zhao YF, Feng ZJ, Xing XY, Wu YH, Chen FH, and Su YZ
- Subjects
- Adult, Aged, Child, Chromosomes, Humans, Janus Kinase 2 genetics, Middle Aged, Myeloproliferative Disorders genetics, Polycythemia Vera genetics, Thrombocythemia, Essential genetics
- Abstract
Objective: To investigate the difference of clinical characteristics between young patients(age≤40 years old) and middle-older patients(age>40 years old) with the myeloproliferative neoplasms(MPN)., Methods: The clinical data (gene mutations, peripheral blood routine examinations, imaging examination and past history) of 269 MPN patients was collected and analyzed., Results: In essential thrombocythemia (ET) group, the proportion of triple-negative type in young patients was higher than that in middle-older group, while the peripheral white blood cell(WBC) and platelets(PLT) counts in the first visit were lower. In polycythemia vera (PV) group, the total detection rate of JAK2V617F (80.65%) was lower than that of other research reports. Young patients with PV showed the lower JAK2V617F rate and lower WBC count, compared with the middle-older aged patients. Both CALR and MPL mutations were not found in PV patients. There was only 1 primary myelofibrosis (PMF) patient aged <40 years old. 91.67% of the patients merged splenomegaly and this rate was higher than that of ET or PV patients. It was found that there were a diagnosed familial MPN family and an undiagnosed family, and the youngest patient was only 8 years old. The second-generation gene sequencing detection for them was not carried out., Conclusion: Age is an important reference index in the assessment of risks. The MPN patients with different age and types show much difference in gene mutations, peripheral blood cell counts, thrombotic events and sizes of spleen. The onset ages of patients with familial MPN trends to be generational younger.
- Published
- 2020
- Full Text
- View/download PDF
44. [Effect of acupuncture on expression of transfer growth factor-β1 in lacrimal gland of rabbits with dry eye].
- Author
-
Liu CY, Qin S, Gao WP, Xi HQ, Xing XY, Ding N, Wei QB, and Xu Q
- Subjects
- Animals, Male, Rabbits, Tears, Transfer Factor, Transforming Growth Factor beta1, Acupuncture Therapy, Dry Eye Syndromes genetics, Dry Eye Syndromes therapy, Lacrimal Apparatus
- Abstract
Objective: To observe the effect of acupuncture on expression of transforming growth factor-β1(TGF-β1) in lacrimal gland of rabbits with dry eye, so as to explore its underlying mechanism in improving dry eye., Methods: Healthy male New Zealand rabbits were randomly assigned to 5 groups ( n =6 in each group), namely, blank group, model group, western medicine group, acupuncture group and sham acupuncture group. The dye eye rabbit model was estabilished by subcutaneous injection of Scopolamine Hydrobromide solution for 21 days. After modeling, rabbits in the western medicine group were treated with Flumirone eye drops in their eyes 3 times a day, one drop each time. Rabbits of the acupuncture group reveived electroacupuncture(4 Hz/20 Hz, 1 mA) at "Cuanzhu"(BL2) and "Tongziliao"(GB1) for 15 min, and received acupuncture at "Jingming"(BL1), "Taiyang" (EX-HN5) and "Sizhukong"(TE23) for 15 min, once a day. Rabbits of the sham acupuncture group received blunt acupuncture at the surface of the same acupoints once a day. All the treatments were conducted for 14 days. The changes of tear flow, tear film break-up time (BUT) and lacrimal gland morphology in each group were observed. The expression of TGF-β1 protein and mRNA in lacrimal gland were detected by Western blot and quantitative real-time fluorescence PCR respectively., Results: Following modeling, except for the blank group, the tear flow and BUT in other 4 groups decreased significantly ( P <0.01). Compared with their own pretreatment, the tear flow and BUT in western medicine group and acupuncture group increased after the treatment ( P <0.05). Compared with the model group, the tear flow and BUT increased in the western medicine group and the acupuncture group( P <0.05). Atrophic lacrimal epithelial cells and the stroma of mucous membrane infiltrated by lymphocytes and plasma cells were found in rabbits of the model group and the sham acupuncture group. By contrast, in the western medicine group and the acupuncture group, the structure of lacrimal epithelial cells was basically normal, and the infiltration of lymphocytes and plasma cells were scattered in the stroma of mucous membrane. In comparison with the blank group, the expression of TGF-β1 protein and mRNA in lacrimal gland were significantly up-regulated in the model and sham acupuncture groups ( P <0.01). Compared with the model group, the expression of TGF-β1 protein and mRNA were significantly down-regulated in the western medicine and acupuncture groups ( P <0.01, P <0.05)., Conclusion: Acupuncture intervention can increase tear flow and BUT in rabbits with dry eye, which may be related to the regulation of TGF-β1 expression in lacrimal gland.
- Published
- 2020
- Full Text
- View/download PDF
45. Repair of Type Ia Endoleaks Involving the Distal Arch Using Left Subclavian Artery-Left Common Carotid Artery Transposition with a Stented Elephant Trunk.
- Author
-
Li CN, Zhu JM, Qi RD, Xing XY, Zhang N, Liu YM, and Sun LZ
- Subjects
- Adult, Aged, Aorta, Thoracic diagnostic imaging, Aortic Aneurysm, Thoracic diagnostic imaging, Blood Vessel Prosthesis Implantation adverse effects, Endoleak diagnostic imaging, Endoleak etiology, Endovascular Procedures adverse effects, Humans, Male, Middle Aged, Prosthesis Design, Retrospective Studies, Risk Factors, Time Factors, Treatment Outcome, Aorta, Thoracic surgery, Aortic Aneurysm, Thoracic surgery, Blood Vessel Prosthesis, Blood Vessel Prosthesis Implantation instrumentation, Carotid Artery, Common surgery, Endoleak surgery, Endovascular Procedures instrumentation, Subclavian Artery surgery
- Abstract
Background: Type Ia endoleaks are common after thoracic endovascular aortic repair (TEVAR). However, the repair of type Ia endoleaks involving the distal arch is challenging because of the presence of the interventional endografts, potential damage to the aortic arch vessels, and the location and size of the aneurysmal body. We retrospectively reviewed our experience of the surgical treatment of type Ia endoleaks with distal arch involvement using left subclavian artery (LSCA)-left common carotid artery (LCCA) transposition with a stented elephant trunk., Methods: Sixteen patients (male = 16; mean age, 47 ± 9 years, range 31-63 years) with type Ia endoleaks involving the distal arch underwent LSCA-LCCA transposition with a stented elephant trunk from July 2010 to July 2018. TEVAR failure occurred in 12 patients, re-TEVAR was performed in two patients, hybrid aortic arch repair in one patient, and the chimney technique in one patient., Results: There were no in-hospital deaths. Fourteen patients required mechanical ventilation for <24 h and one for <48 h. One patient required reintubation after mechanical ventilation for 19 h and continuous renal replacement therapy because of renal failure. One patient received pericardial drainage, and recurrent laryngeal nerve injury occurred in one patient. Three patients died during follow-up., Conclusions: The LSCA-LCCA transposition with a stented elephant trunk can produce satisfactory results in patients with a type Ia endoleak involving the distal arch. Using this technique, it is possible to exclude the aneurysm sac distal to the LCCA origin and seclude the failed interventional endograft. These encouraging outcomes suggested that this technique could be a suitable surgical treatment for this type of lesion., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
46. Jinbei Oral Liquid ameliorates bleomycin-induced idiopathic pulmonary fibrosis in rats via reversion of Th1/Th2 shift.
- Author
-
Xing XY, Qiang WJ, Bao JL, Yang RC, Hou J, Tao K, Meng ZQ, Zhang JH, Zhang AJ, and Sun XB
- Abstract
Objective: Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal interstitial lung disease with high mortality. The pivotal role of Th1/Th2 immunological balance in the development and progression of IPF has been demonstrated previously. This study aimed to evaluate the effect of Jinbei Oral Liquid (JBOL) on IPF and its relationship with Th1/Th2 shift., Methods: Rats were divided into six groups: control group, model group (bleomycin), pirfenidone group (positive group, 54 mg/kg, i.g.) and JBOL (5.4, 10.8 and 21.6 mL/kg, i.g.) groups. The rat model was established by an intratracheal instillation of bleomycin (BLM, 5 mg/kg). One day after injection of BLM, pirfenidone or JBOL was given to rats once daily within 28 consecutive days, respectively. Positron emission tomography/computed tomography (PET/CT) was performed on the treated rats. The extent of alveolitis and fibrosis was observed by H&E and Masson trichrome staining. The contents of TGF-β1, TNF-α, IL-4 and IFN-γ were further quantified by ELISA assay., Results: PET/CT and histopathological evidence showed the ability of JBOL to attenuate bleomycin-induced alveolitis and fibrosis extent, and the alveolitis lesion score was markedly decreased compared with the model group. The increased expression of inflammatory cytokines TGF-β1 and TNF-α induced by bleomycin was also suppressed by JBOL. The Th1 response was limited by the reduced IFN-γ after BLM administration, and the Th2 response predominated significantly marked by the increased IL-4. JBOL could increase the level of IFN-γ and markedly increased the ratio of IFN-γ/IL-4., Conclusion: These findings suggested that JBOL may attenuate BLM-induced idiopathic pulmonary fibrosis via reducing inflammatory cell infiltration, pro-inflammatory cytokine release and excessive collagen deposition in rats. One of the mechanisms is the reversion of Th1/Th2 shift caused by BLM., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2020 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V.)
- Published
- 2020
- Full Text
- View/download PDF
47. Mortality and Disease Burden of Injuries from 2008 to 2017 in Anhui Province, China.
- Author
-
Xing XY, Wang P, Xu Z, He Q, Li R, Chen YJ, Liu LN, Mao YM, Zhao CN, Dan YL, Wu Q, Pan HF, Liu ZR, and Hu W
- Subjects
- Adolescent, Adult, Cause of Death, Child, Child, Preschool, China epidemiology, Cost of Illness, Female, Humans, Infant, Infant, Newborn, Male, Middle Aged, Accidental Falls mortality, Accidents, Traffic mortality, Drowning mortality, Suicide, Wounds and Injuries mortality
- Abstract
Objective: To investigate the temporal trends in mortality and disease burden of injuries in Anhui province from 2008 to 2017, so as to provide reference for injury control and prevention., Methods: Data of mortality were collected from 9 national surveillance points in Anhui province during 2008-2017 in the Information System for Death Cause Register and Management. The surveillance data were analyzed by using crude mortality, standardized mortality rate (SMR), potential year of life lost (PYLL), PYLL rate (PYLLR), and average of year life lost (AYLL)., Results: There were a total of 44855 people died from injury, accounted for 9.44% of the all-cause mortality, ranked as the fifth leading cause of deaths in the whole population, and denoted the first leading cause of deaths in the 0-44 year's group. The leading causes of injury deaths were road traffic accidents, suicide, accidental falls, drowning, and poisoning. Road traffic accidents was the primary cause of injury deaths among the male population, while suicide was the dominate cause of injury deaths among the female population. Drowning, traffic accidents, and suicide accounted for the most injury deaths among the population aged 0-14 years, 15-64 years, and above 60 years, respectively. The road traffic accidents accounted for the largest proportion of injury PYLL and PYLLR, and drowning caused the highest AYLL among injury deaths., Conclusion: In Anhui province, road traffic accidents, suicide, accidental falls, drowning, and poisoning were the top five causes of injury deaths that harm the health of local residents; corresponding injury prevention strategies should be formulated., Competing Interests: The authors declared that they have no conflicts of interests., (Copyright © 2020 Xiu-Ya Xing et al.)
- Published
- 2020
- Full Text
- View/download PDF
48. [A cross-sectional study on the prevalence and related factors of dyslipidemia among adults in Anhui province, in 2015].
- Author
-
Xu W, Xing XY, He Q, Dai D, Li R, Xu JQ, Chen YJ, and Liu ZR
- Subjects
- Adult, China epidemiology, Cross-Sectional Studies, Female, Humans, Male, Prevalence, Risk Factors, Dyslipidemias epidemiology
- Abstract
Objective: Based on the data of chronic diseases and nutrition surveillance among Chinese adults in 2015, dyslipidemia and related factors were analyzed. Methods: Multi-stage stratified cluster random sampling was used to select participants who were aged 18 and over, with questionnaire survey and related measurements conducted. Prevalence rates of dyslipidemia among participants by different characteristics and influencing factors were analyzed, using the method of complex weighting and post-weighted stratification. Results: In all, 7 404 participants were included. The overall prevalence of dyslipidemia was 30.5 % among the adults. The overall prevalence of dyslipidemia were 36.5 % in males and 24.4 % in females ( P <0.05). The prevalence rates of hypercholesterolemia, hypertriglyceridemia, high LDL-C, and low HDL-C were 3.7 % , 12.2 % , 5.3 % , and 19.4 % , respectively. Results from the multivariate logistic regression model analysis showed that age ( OR =1.009, 95 %CI : 1.000-1.018), female ( OR =0.501, 95 %CI : 0.397-0.632), College degree or above ( OR =1.728, 95 %CI : 1.257-2.374), alcohol consumption 3 ( OR =0.711, 95 %CI : 0.536-0.943), central obesity ( OR =1.868, 95 %CI : 1.547-2.257), BMI ( OR =1.141, 95 %CI : 1.098-1.186), hypertension ( OR =1.259, 95 %CI : 1.077-1.473) and diabetes ( OR =2.025, 95 %CI : 1.446-2.835) were influencing factors on dyslipidemia. Conclusions: The prevalence of dyslipidemia seemed high among adults in Anhui. Risk factors should be closely monitored and under control, including those people with unhealthy lifestyles or being overweight, obesity, hypertensive and diabetic.
- Published
- 2020
- Full Text
- View/download PDF
49. Oct-4 Enhanced the Therapeutic Effects of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Acute Kidney Injury.
- Author
-
Zhang ZY, Hou YP, Zou XY, Xing XY, Ju GQ, Zhong L, and Sun J
- Subjects
- Acute Kidney Injury pathology, Animals, Disease Models, Animal, Extracellular Vesicles metabolism, Humans, Immunohistochemistry, Male, Mesenchymal Stem Cells cytology, Mice, Mice, Inbred C57BL, Octamer Transcription Factor-3 biosynthesis, Random Allocation, Acute Kidney Injury therapy, Mesenchymal Stem Cell Transplantation methods, Mesenchymal Stem Cells metabolism, Octamer Transcription Factor-3 genetics
- Abstract
Background/aims: Acute kidney injury (AKI) is a common clinical condition that can lead to chronic kidney failure. Although mesenchymal stem cell-derived extracellular vesicles (MSC EVs) are regarded as a potent AKI treatment, the mechanisms underlying their beneficial effects remain unclear. Oct-4 may play an important role in tissue injury repair. We thus hypothesized that oct-4 overexpression might enhance the therapeutic effects of MSC EVs in AKI treatment., Methods: Renal tubular epithelial cells were cultured in a low oxygen environment, then cocultured with MSC EVs or control medium for 48 h. BrdU and transferase-mediated dUTP nick-end labeling (TUNEL) staining were used to assess cell proliferation and apoptosis. Mice subjected to ischemia reperfusion were randomly divided into 4 groups, then injected with either phosphate-buffered saline (vehicle), EVs, EVs overexpressing oct-4 (EVs+Oct-4), and EVs not expressing Oct-4 (EVs-Oct-4). Blood creatinine (CREA) and urine nitrone levels were assessed 48 h and 2 weeks after injection. After ischemia reperfusion, renal tissues from each group were stained with TUNEL and proliferating cell nuclear antigen (PCNA) to determine the degree of apoptosis and proliferation. Masson trichrome staining was used to evaluate renal fibrosis progression. Snail gene expression was assessed using polymerase chain reaction (PCR)., Results: At 48 h after hypoxic treatment, TUNEL and BrdU staining indicated that the EVs+Oct-4 group had the least apoptosis and the most proliferation, respectively. Treatment with EVs overexpressing Oct-4 significantly decreased serum Crea and blood urea nitrogen levels and rescued kidney fibrosis, as indicated by the low proportion of Masson staining, high number of PCNA-positive cells, and low number of TUNEL-positive cells. PCR analysis indicated that Snail was most upregulated in the vehicle group and least upregulated in the EVs+Oct-4 group., Conclusions: MSC EVs had a pronounced therapeutic effect on ischemic reperfusion injury-related AKI, and Oct-4 overexpression enhanced these therapeutic effects. Our results may inspire a new direction for AKI treatment with MSC EVs., (© 2020 The Author(s) Published by S. Karger AG, Basel.)
- Published
- 2020
- Full Text
- View/download PDF
50. LncRNA CASC2 regulates high glucose-induced proliferation, extracellular matrix accumulation and oxidative stress of human mesangial cells via miR-133b/FOXP1 axis.
- Author
-
Zhang XL, Zhu HQ, Zhang Y, Zhang CY, Jiao JS, and Xing XY
- Subjects
- Cell Line, Cell Proliferation drug effects, Cell Proliferation physiology, Diabetic Nephropathies genetics, Diabetic Nephropathies metabolism, Diabetic Nephropathies pathology, Extracellular Matrix drug effects, Extracellular Matrix genetics, Forkhead Transcription Factors genetics, Glucose toxicity, Humans, Mesangial Cells drug effects, MicroRNAs genetics, Oxidative Stress drug effects, Repressor Proteins genetics, Tumor Suppressor Proteins genetics, Extracellular Matrix metabolism, Forkhead Transcription Factors biosynthesis, Mesangial Cells metabolism, MicroRNAs biosynthesis, Oxidative Stress physiology, Repressor Proteins biosynthesis, Tumor Suppressor Proteins biosynthesis
- Abstract
Objective: Diabetic nephropathy (DN) is one of the primary complications of diabetes. Long non-coding RNA cancer susceptibility candidate 2 (CASC2) has been established to function in DN, while its role in high glucose (HG)-induced human mesangial cells (HMCs) remains limited., Materials and Methods: The expression level of CASC2 and miR-133b was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was assessed using cell counting kit-8 (CCK-8) assay. Extracellular matrix (ECM) accumulation was monitored through the expression levels of collagen IV (Col IV) and fibronectin (FN) using qRT-PCR and western blot analyses. Oxidative stress was observed through the expression of NADPH oxidase2 (NOX2) and the activity of malondialdehyde (MDA) and superoxide dismutase (SOD) using western blot or corresponding detection kit. The expression of forkhead box P1 (FOXP1) at mRNA and protein levels was determined by qRT-PCR and Western blot, respectively. The relationship between miR-133b and CASC2 or FOXP1 was predicted by online bioinformatics tools and verified by dual-luciferase reporter assay or RNA pull-down., Results: The expression of CASC2 was reduced in serum from DN patients and HG-induced HMCs. CASC2 upregulation inhibited HG-induced HMCs proliferation, ECM accumulation and oxidative stress. MiR-133b was a target of CASC2 with a high level in serum from DN patients and HG-induced HMCs, and its enrichment reversed the effects of CASC2 upregulation. Besides, FOXP1 was a target of miR-133b with a low level in HG-induced HMCs, and its knockdown abolished the impacts of CASC2 upregulation., Conclusions: CASC2 upregulation suppressed HG-induced proliferation, ECM accumulation and oxidative stress of HMCs through miR-133b /FOXP1 regulatory axis, suggesting that CASC2 was a novel biomarker for DN treatment.
- Published
- 2020
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.