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2 results on '"XinNian Fu"'

1. Effect and mechanism of protease inhibitor oprozomib on doxorubicin sensitivity of hepatocellular carcinoma cells

Author

Feng Qi, Sheng Zheng, Lu Hou, XinNian Fu, XinXin Wang, ZhiPeng Fu, and Juan Yang

Subjects
doxorubicin, hepatocellular carcinoma, drug resistance, protease inhibitor, oprozomib, proapoptotic gene, Pharmacy and materia medica, RS1-441
Abstract

This study was to examine the impact of Oprozomib (OPZ), an oral second-generation proteasome inhibitor, on the sensitivity of hepatocellular carcinoma (HCC) cells to doxorubicin (DOX), a commonly used chemotherapy drug for the treatment of HCC. HCC cells were incubated with OPZ or DOX. Cell proliferation was assessed using MTT and colony formation assay, while cell migration and invasion were evaluated using scratch assay and Transwell assay. Cell apoptosis was measured using flow cytometry, and apoptosis-related molecules were determined through Western blot analysis. The proliferation, migration, and invasion of HCC cells were effectively suppressed by OPZ in a dose-dependent manner. This inhibition was achieved through the upregulation of pro-apoptotic genes, resulting in the promotion of apoptosis. Furthermore, OPZ significantly augmented the cytotoxicity and apoptosis induced by DOX. OPZ alone can effectively inhibit cell proliferation and induce apoptosis. OPZ can enhance DOX sensitivity and cellular apoptosis.

Published
2024
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2. Development of simple sequence repeat markers and diversity analysis in alfalfa (Medicago sativa L.)

Author

Hongwen Gao, Zan Wang, Xuehui Li, Xinnian Fu, and Hongwei Yan

Subjects
Molecular breeding, Genetics, Expressed Sequence Tags, Genetic Markers, Genetic diversity, Expressed sequence tag, Polymorphism, Genetic, Sequence analysis, food and beverages, Genetic Variation, General Medicine, Sequence Analysis, DNA, Biology, Quantitative trait locus, Genetic marker, Botany, Genetic variation, Medicago sativa, Molecular Biology, Microsatellite Repeats
Abstract

Efficient and robust molecular markers are essential for molecular breeding in plant. Compared to dominant and bi-allelic markers, multiple alleles of simple sequence repeat (SSR) markers are particularly informative and superior in genetic linkage map and QTL mapping in autotetraploid species like alfalfa. The objective of this study was to enrich SSR markers directly from alfalfa expressed sequence tags (ESTs). A total of 12,371 alfalfa ESTs were retrieved from the National Center for Biotechnology Information. Total 774 SSR-containing ESTs were identified from 716 ESTs. On average, one SSR was found per 7.7 kb of EST sequences. Tri-nucleotide repeats (48.8 %) was the most abundant motif type, followed by di-(26.1 %), tetra-(11.5 %), penta-(9.7 %), and hexanucleotide (3.9 %). One hundred EST-SSR primer pairs were successfully designed and 29 exhibited polymorphism among 28 alfalfa accessions. The allele number per marker ranged from two to 21 with an average of 6.8. The PIC values ranged from 0.195 to 0.896 with an average of 0.608, indicating a high level of polymorphism of the EST-SSR markers. Based on the 29 EST-SSR markers, assessment of genetic diversity was conducted and found that Medicago sativa ssp. sativa was clearly different from the other subspecies. The high transferability of those EST-SSR markers was also found for relative species.

Published
2012

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