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This study aimed to explore the mechanism of action of LINC01133 in non-small cell lung cancer. LINC01133 expression in NSCLC patient tissues and cells was detected by qRT-PCR. After transfecting siRNA-LINC01133 in NSCLC cells, the proliferation and invasive migration ability of the cells were assessed via CCK-8 and Transwell assay, respectively. The sublocalization of LINC01133 in NSCLC cells was analyzed by bioinformatics prediction and nucleoplasm separation assay and RNA-FISH assay. Analysis of the binding relationship between LINC01133, FOXA1 and miR-30b-5p was all through bioinformatics website analysis, dual-luciferase reporter and RNA Pulldown assay. Functional rescue experiments confirmed the character of miR-30b-5p and FOXA1 in LINC01133 regulating the NSCLC cells biological behavior. LINC01133 high expressions were found in NSCLC tissues and cells. siRNA-LINC01133 treatment inhibited NSCLC cells malignant behavior. Mechanistically: LINC01133 promoted FOXA1 expression through adsorption binding of miR-30b-5p. Knocking down miR-30b-5p expression or up-regulating FOXA1 expression was able to reverse siRNA-LINC01133 inhibitory effect of tumor cell malignant behavior. LINC01133 promoted FOX1 expression by competitively binding miR-30b-5p, which attenuated the targeting inhibitory effect of miR-30b-5p on FOXA1 and ultimately promoted proliferation and invasive migration of NSCLC cells.

Purpose: We aimed to explore the efficacy and safety of postoperative adjuvant radiotherapy in the treatment of non-small cell lung cancer (NSCLC) (stage IIIA-N2), and to analyze the influencing factors for the prognosis of patients., Methods: A total of 142 patients with NSCLC (stage IIIA-N2) were collected for retrospective analysis. Postoperative adjuvant radiotherapy was performed in 71 cases (Radiotherapy group), while it was not conducted in the remaining 71 cases (Control group). The survival status of patients was recorded during follow-up. Moreover, the possible influencing factors for the prognosis of patients were analyzed., Results: The median survival time was 34.7±5.4 months and 31.9±4.9 months, the 5-year overall survival (OS) rate was 32.4% and 26.8%, and the 5-year progression-free survival (PFS) rate was 25.4% and 12.7%, respectively, in the Radiotherapy group and the Control group. The 5-year OS was significantly correlated with smoking history, tumor T stage, ratio of positive lymph nodes, number of cycles of postoperative chemotherapy, and whether postoperative adjuvant radiotherapy was combined. Moreover, tumor T stage, ratio of positive lymph nodes and whether adjuvant radiotherapy was combined were independent influencing factors for postoperative OS of patients. The lower tumor T stage, lower ratio of positive lymph nodes and adjuvant radiotherapy combined corresponded to the higher OS rate., Conclusions: Postoperative adjuvant radiotherapy is safe and feasible in the treatment of NSCLC (stage IIIA-N2), which can increase the survival of patients and the local control rate of tumors. Patients with a lower tumor T stage and a lower ratio of positive lymph nodes have higher survival rates.