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13. Genetic Mapping, Candidate Gene Identification and Marker Validation for Host Plant Resistance to the Race 4 of Fusarium oxysporum f. sp. cubense Using Musa acuminata ssp. malaccensis

Author

Chen, Andrew, primary, Sun, Jiaman, additional, Viljoen, Altus, additional, Mostert, Diane, additional, Xie, Yucong, additional, Mangila, Leroy, additional, Bothma, Sheryl, additional, Lyons, Rebecca, additional, Hřibová, Eva, additional, Christelová, Pavla, additional, Uwimana, Brigitte, additional, Amah, Delphine, additional, Pearce, Stephen, additional, Chen, Ning, additional, Batley, Jacqueline, additional, Edwards, David, additional, Doležel, Jaroslav, additional, Crisp, Peter, additional, Brown, Allan F., additional, Martin, Guillaume, additional, Yahiaoui, Nabila, additional, D’Hont, Angelique, additional, Coin, Lachlan, additional, Swennen, Rony, additional, and Aitken, Elizabeth A. B., additional

Published
2023
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16. Genetic mapping, candidate gene identification and marker validation for host plant resistance to the race 4 of Fusarium oxysporum f. sp. cubense using Musa acuminata ssp. malaccensis

Author

Chen, Andrew, Sun, Jiaman, Viljoen, Altus, Mostert, Diane, Xie, Yucong, Mangila, Leroy, Bothma, Sheryl, Lyons, Rebecca, Hribova, Eva, Christelová, Pavla, Uwimana, Brigitte, Amah, Delphine, Pearce, Stephen, Chen, Ning, Batley, Jacqueline, Edwards, David P., Dolezel, Jaroslav, Crisp, Peter, Brown, Allan F., Martin, Guillaume, Yahiaoui, Nabila, D'Hont, Angélique, Coin, Lachlan, Swennen, Rony, Aitken, Elizabeth A. B., Chen, Andrew, Sun, Jiaman, Viljoen, Altus, Mostert, Diane, Xie, Yucong, Mangila, Leroy, Bothma, Sheryl, Lyons, Rebecca, Hribova, Eva, Christelová, Pavla, Uwimana, Brigitte, Amah, Delphine, Pearce, Stephen, Chen, Ning, Batley, Jacqueline, Edwards, David P., Dolezel, Jaroslav, Crisp, Peter, Brown, Allan F., Martin, Guillaume, Yahiaoui, Nabila, D'Hont, Angélique, Coin, Lachlan, Swennen, Rony, and Aitken, Elizabeth A. B.

Abstract

Fusarium wilt of banana is a devastating disease that has decimated banana production worldwide. Host resistance to Fusarium oxysporum f. sp. Cubense (Foc), the causal agent of this disease, is genetically dissected in this study using two Musa acuminata ssp. Malaccensis segregating populations, segregating for Foc Tropical (TR4) and Subtropical (STR4) race 4 resistance. Marker loci and trait association using 11 SNP-based PCR markers allowed the candidate region to be delimited to a 12.9 cM genetic interval corresponding to a 959 kb region on chromosome 3 of 'DH-Pahang' reference assembly v4. Within this region, there was a cluster of pattern recognition receptors, namely leucine-rich repeat ectodomain containing receptor-like protein kinases, cysteine-rich cell-wall-associated protein kinases, and leaf rust 10 disease-resistance locus receptor-like proteins, positioned in an interspersed arrangement. Their transcript levels were rapidly upregulated in the resistant progenies but not in the susceptible F2 progenies at the onset of infection. This suggests that one or several of these genes may control resistance at this locus. To confirm the segregation of single-gene resistance, we generated an inter-cross between the resistant parent 'Ma850' and a susceptible line 'Ma848', to show that the STR4 resistance co-segregated with marker '28820' at this locus. Finally, an informative SNP marker 29730 allowed the locus-specific resistance to be assessed in a collection of diploid and polyploid banana plants. Of the 60 lines screened, 22 lines were predicted to carry resistance at this locus, including lines known to be TR4-resistant, such as 'Pahang', 'SH-3362', 'SH-3217', 'Ma-ITC0250', and 'DH-Pahang/CIRAD 930'. Additional screening in the International Institute for Tropical Agriculture's collection suggests that the dominant allele is common among the elite 'Matooke' NARITA hybrids, as well as in other triploid or tetraploid hybrids derived from East African highland

Published
2023

23. Induced mutations in ASPARAGINE SYNTHETASE‐A2reduce free asparagine concentration in the wheat grain

Author

Alarcón‐Reverte, Rocío, Xie, Yucong, Stromberger, John, Cotter, Jennifer D., Mason, Richard Esten, and Pearce, Stephen

Abstract

Acrylamide is a neurotoxin and probable carcinogen formed as a processing contaminant during baking and production of different foodstuffs, including bread products. The amino acid asparagine is the limiting substrate in the Maillard reaction that produces acrylamide, so developing wheat (Triticum aestivumL.) cultivars with low free asparagine concentrations in the grain is a promising approach to reduce dietary acrylamide exposure. A candidate gene approach was used to identify chemically induced genetic variation in ASPARAGINE SYNTHETASE 2(ASN2) genes that exhibit a grain‐specific expression profile. In field trials, durum and common wheat lines carrying asn‐a2null alleles exhibited reductions in free asparagine concentration in their grains of between 9 and 34% compared with wildtype sister lines. These plants showed no significant differences in spikelet number, grain size and weight, germination or baking quality traits. These nontransgenic variants can be deployed without regulatory oversight in elite wheat germplasm to reduce acrylamide‐forming potential with no negative effects on quality or agronomic performance. Three wheat ASPARAGINE SYNTHETASE 2knockout alleles were characterized in field experiments.Mutant alleles conferred significant reductions in grain free asparagine concentration.The alleles did not affect quality or agronomic traits.

Published
2022
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