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PHB3 regulates lateral root primordia formation via NO-mediated degradation of AUXIN/INDOLE-3-ACETIC ACID proteins ACID proteins. The molecular network of PHB3 and NO in regulating LR initiation Prohibitin 3 (PHB3) induces nitric oxide (NO) accumulation to promote lateral root (LR) initiation. This process is activated by an auxin/indole-3-acetic acid (AUX/IAA)-AUXIN RESPONSE FACTOR (ARF)-dependent auxin signaling cascade. Keywords: Auxin; founder cell; lateral root; nitric oxide; PHB3 EN Auxin founder cell lateral root nitric oxide PHB3 3828 3830 3 06/28/22 20220624 NES 220624 B Plant lateral roots (LRs) initiate when a small group of pericycle cells are primed to undergo cell division to form LR primordia (LRPs). [Extracted from the article]

SUMMARY: High levels of ammonium nutrition reduce plant growth and different plant species have developed distinct strategies to maximize ammonium acquisition while alleviating ammonium toxicity through modulating root growth. To date, the mechanisms underlying plant tolerance or sensitivity towards ammonium remain unclear. Rice (Oryza sativa) uses ammonium as its main N source. Here we show that ammonium supply restricts rice root elongation and induces a helical growth pattern, which is attributed to root acidification resulting from ammonium uptake. Ammonium‐induced low pH triggers the asymmetric distribution of auxin in rice root tips through changes in auxin signaling, thereby inducing a helical growth response. Blocking auxin signaling completely inhibited this root response. In contrast, this root response is not activated in ammonium‐treated Arabidopsis. Acidification of Arabidopsis roots leads to the protonation of indole‐3‐acetic acid and dampening of the intracellular auxin signaling levels that are required for maintaining root growth. Our study suggests a different mode of action by ammonium on the root pattern and auxin response machinery in rice versus Arabidopsis, and the rice‐specific helical root response towards ammonium is an expression of the ability of rice to moderate auxin signaling and root growth to utilize ammonium while confronting acidic stress. Significance Statement: In this study, we discovered a helical response of the rice root in response to the acidic stress resulting from root ammonium uptake. Ammonium‐induced acidic stress triggers the asymmetric distribution of auxin at rice root tips through modulating auxin signaling pathways, thereby inducing a helical root response. [ABSTRACT FROM AUTHOR]

A biological clock activated by oscillating signals, known as root clock, has been linked to lateral root (LR) formation and is essential for regular LR spacing along the primary root. However, it remains unclear how this internal mechanism is influenced by environmental factors known to affect the LR pattern. Here, we report that excessive cadmium (Cd) inhibits LR formation by disrupting the lateral root cap (LRC)‐programmed cell death (PCD)‐regulated root clock. Cd restricts the frequency of the oscillating signal rather than its amplitude. This could be attributed to the inhibition on meristematic activity by Cd, which resulted in decreased LRC cell number and LRC‐PCD frequency. Genetic evidence further showed that LRC cell number is positively correlated with root resistance to Cd. Our study reveals root cap dynamics as a novel mechanism mediating root responses to Cd, providing insight into the signalling pathways of the root clock responding to environmental cues. In this study, we report that heavy metal cadmium (Cd) stress represses the periodic lateral root formation by reducing cell division activity in the root apical meristem, which on its turn restricts the lateral root cap (LRC)‐programmed cell death (PCD)‐triggered DR5 oscillations in the oscillation zone. Our genetic evidence also reveals a positive correlation between LRC cell number and root resistance to Cd. These findings provide novel insights to the mechanism underlying Cd‐inhibited LR formation and improve our understanding on LR patterning responding to environmental cues. [ABSTRACT FROM AUTHOR]

Lateral root branching along the primary root involves complex gene regulatory networks in model plant Arabidopsis. However, it is largely unclarified whether different plant species share a common mechanism to pattern the lateral root along the primary axis. In this study, we assessed the development pattern of lateral root among several dicot and monocot plants, including Arabidopsis, tomato, Medicago , Nicotiana , rice, and ryegrass by using an agar-gel culture system. Our results reveal a regular-spaced distribution pattern of lateral roots along the primary root axis of both dicot and monocot plants. Meanwhile, the root patterning is tightly controlled by root bending and the plant hormone auxin. However, nitrogen and phosphate starvations trigger distinguished root growth patterns among different plant species. Our studies strongly suggest a partially shared signaling pathway underlying root patterning of various plant species, and also provide a foundation for further identification of genes associated with root development. [ABSTRACT FROM AUTHOR]

Accurate estimation for vegetation net primary production (NPP) at high resolution is essential for ecosystem management and at local to global scales. However, no reliable methods are available to achieve fine-resolution estimation. In this study, a new NPP estimation system was developed by integrating high spatial resolution remote sensing images, tree ring measurements, and process-based model CASA. The system was illustrated by estimating the historical NPP of caragana shrublands from 2011 to 2021 in Dingbian County, China. The results showed that: (1) by employing the biomass model and tree ring measurements, an NPP-crown width model was established to enable a large number of samples for calibrating the CASA model on high spatial resolution images without continuous field measurements. The calibrated parameters, including maximum NDVI, minimum NDVI, and maximum light use efficiency, were determined to be 0.415, 0.022 and 0.025, respectively; (2) according to the estimation by the calibrated CASA model, the NPP in Dingbian County revealed an increasing trend from 2011 to 2021, and the NPP in southern hilly and gully regions was significantly higher than the other regions; (3) among the different indices, max temperature of warmest month had a significant negative effect on the NPP of cagarana shrublands, whereas annual precipitation had a positive effect. In the northern and central regions, air temperature predominantly influenced NPP, while in the southern regions, both temperature and precipitation played a joint role. The implementation in this study illustrated that the NPP estimation system could provide reliable NPP with high resolution and efficient to monitor and detect dominant factors affecting NPP for ecosystem management. [ABSTRACT FROM AUTHOR]

Lateral root formation is coordinated by both endogenous and external factors. As biotic factors, plant growth‐promoting rhizobacteria can affect lateral root formation, while the regulation mechanism is unclear. In this study, by applying various marker lines, we found that volatile compounds (VCs) from Bacillus amyloliquefaciens SQR9 induced higher frequency of DR5 oscillation and prebranch site formation, accelerated the development and emergence of the lateral root primordia and thus promoted lateral root development in Arabidopsis. We demonstrated a critical role of auxin on B. amyloliquefaciens VCs‐induced lateral root formation via respective mutants and pharmacological experiments. Our results showed that auxin biosynthesis, polar transport and signalling pathway are involved in B. amyloliquefaciens VCs‐induced lateral roots formation. We further showed that acetoin, a major component of B. amyloliquefaciens VCs, is less active in promoting root development compared to VC blends from B. amyloliquefaciens, indicating the presence of yet uncharacterized/unknown VCs might contribute to B. amyloliquefaciens effect on lateral root formation. In summary, our study revealed an auxin‐dependent mechanism of B. amyloliquefaciens VCs in regulating lateral root branching in a non‐contact manner, and further efforts will explore useful VCs to promote plant root development. The volatile compounds from Bacillus spp. increase the frequency of prebranch site formation and further accelerated the emergence of lateral root primordia (LRP) from the primary root (PR) in Arabidopsis, resulting in a denser lateral root (LR) along the PR spatially. This process is dependent on auxin signaling pathway; fully functional machinery of YUCs‐mediated auxin biosynthesis and polar auxin transport are also required. [ABSTRACT FROM AUTHOR]

The lack of information on the correlation between root causes and corresponding control criteria in the importance calculation of root causes of design problems results in less accurate determinations of core problems. Based on the interaction between customer needs, bad product parameters, and root causes, a hierarchical representation model of the design problem is established in this paper. A network layer of bad parameters, including various types of correlations, and a control layer, including technical feasibility and cost, are constructed. Then, a method based on the network analytic hierarchy process is proposed to rank the importance of root causes of the design problem and determine the core problems. Finally, a product design process based on the core problem solving is established to assist designers with improving design quality and efficiency. The design for the coolant flow distribution device in the lower chamber of a third-generation pressurized water reactor is employed as an example to demonstrate the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

Summary: Lateral root (LR) positioning and development rely on the dynamic interplay between auxin production, transport but also inactivation. Nonetheless, how the latter affects LR organogenesis remains largely uninvestigated.Here, we systematically analyze the impact of the major auxin inactivation pathway defined by GRETCHEN HAGEN3‐type (GH3) auxin conjugating enzymes and DIOXYGENASE FOR AUXIN OXIDATION1 (DAO1) in all stages of LR development using reporters, genetics and inhibitors in Arabidopsis thaliana.Our data demonstrate that the gh3.1/2/3/4/5/6 hextuple (gh3hex) mutants display a higher LR density due to increased LR initiation and faster LR developmental progression, acting epistatically over dao1‐1. Grafting and local inhibitor applications reveal that root and shoot GH3 activities control LR formation. The faster LR development in gh3hex is associated with GH3 expression domains in and around developing LRs. The increase in LR initiation is associated with accelerated auxin response oscillations coinciding with increases in apical meristem size and LR cap cell death rates.Our research reveals how GH3‐mediated auxin inactivation attenuates LR development. Local GH3 expression in LR primordia attenuates development and emergence, whereas GH3 effects on pre‐initiation stages are indirect, by modulating meristem activities that in turn coordinate root growth with LR spacing. [ABSTRACT FROM AUTHOR]

Crown roots (CRs) are major components of the rice root system. They form at the basal node of the shoot, and their development is greatly influenced by environmental factors. Ammonium nitrogen is known to impact plant root development through ammonium transporters (AMTs), but it remains unclear whether ammonium and AMTs play roles in rice CR formation. In this study, we revealed a significant role of ammonium, rather than nitrate, in regulating rice CR development. High ammonium supply increases CR formation but inhibits CR elongation. Genetic evidence showed that ammonium regulation of CR development relies on ammonium uptake mediated jointly by ammonium transporters OsAMT1;1, OsAMT1;2; OsAMT1;3, and OsAMT2;1, but not on root acidification which was the result of ammonium uptake. OsAMTs are also needed for glutamine-induced CR formation. Furthermore, we showed that polar auxin transport dependent on the PIN auxin efflux carriers acts downstream of ammonium uptake and assimilation to activate local auxin signaling at CR primordia, in turn promoting CR formation. Taken together, our results highlight a critical role for OsAMTs in cooperatively regulating CR formation through regulating auxin transport under nitrogen-rich conditions. [ABSTRACT FROM AUTHOR]

The spacing of lateral roots (LRs) along the main root in plants is driven by an oscillatory signal, often referred to as the "root clock" that represents a pre-patterning mechanism that can be influenced by environmental signals. Light is an important environmental factor that has been previously reported to be capable of modulating the root clock, although the effect of light signaling on the LR pre-patterning has not yet been fully investigated. In this study, we reveal that light can activate the transcription of a photomorphogenic gene HY1 to maintain high frequency and amplitude of the oscillation signal, leading to the repetitive formation of pre-branch sites. By grafting and tissue-specific complementation experiments, we demonstrated that HY1 generated in the shoot or locally in xylem pole pericycle cells was sufficient to regulate LR branching. We further found that HY1 can induce the expression of HY5 and its homolog HYH , and act as a signalosome to modulate the intracellular localization and expression of auxin transporters, in turn promoting auxin accumulation in the oscillation zone to stimulate LR branching. These fundamental mechanistic insights improve our understanding of the molecular basis of light-controlled LR formation and provide a genetic interconnection between shoot- and root-derived signals in regulating periodic LR branching. [Display omitted] • Light is essential for maintaining high oscillation signal and LR formation • HY1 mediates the effects of light signals in oscillation and pre-branch site formation • Both shoot- and root-derived HY1 are critical for its role in regulating LR branching • HY5 and auxin act downstream of HY1 to promote LR formation In plants, periodic lateral root (LR) formation is driven by a gene oscillatory signal. Duan et al. report a critical role of light in maintaining high oscillation signals for subsequent pre-branch site and LR formation, and identify a light-responsive HY1-HY5-auxin signaling cascade that regulates oscillation signals and periodic LR formation. [ABSTRACT FROM AUTHOR]

At present, analysis and design methods for a product's carbon footprint are focused mostly on its existing structures, which are difficult to integrate with the conceptual design process of low-carbon, innovative products. To solve this problem, this study proposes a new low-carbon design method based on a multi-layer carbon footprint information model. The hierarchical carbon footprint information model is the combination of direct structural design elements with indirect design elements such as functions and principles. The study also proposes a method for qualitative/semi-quantitative carbon footprint calculation. To achieve this, design information is combined with product structure tree (PST) to form a greenhouse gas (GHG)-product structure tree (G-PST) based on the carbon footprint design information. The influence degree (Id) of each design element in the G-PST is evaluated by the analytical network process (ANP) method and the optimizable degree (Od) of each design element for low-carbon design is obtained. The low-carbon product design is divided into four categories—structure optimization design, principle optimization design, function optimization design, and process optimization design—and the corresponding innovative design strategies are proposed. The results of this research can effectively obtain and standardize various low-carbon design elements, and provide targeted methods and tools for guiding designers to implement innovative low-carbon designs. The effectiveness of this low-carbon design method proposed is then tested on a Haier automatic washing machine, EB72M2JD. • A model for studying carbon footprint from multi-layer elements was provided. • Two evaluation dimensions to identify target elements at each layer were proposed. • Four types of low-carbon design were studied since the core issues are multifaceted. • Corresponding design strategies for various low-carbon design issues were proposed. [ABSTRACT FROM AUTHOR]