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33 results on '"Xie, Suqing"'

2. Chemical Synthesis and Antigenicity Evaluation of an Aminoglycoside Trisaccharide Repeating Unit of Pseudomonas aeruginosaSerotype O5 O-Antigen Containing a Rare Dimeric-ManpN3NA

Author

Tian, Guangzong, Hu, Jing, Qin, Chunjun, Li, Lingxin, Ning, Yunzhan, Zhu, Shengyong, Xie, Suqing, Zou, Xiaopeng, Seeberger, Peter H., and Yin, Jian

Abstract

Pseudomonas aeruginosabacteria are becoming increasingly resistant against multiple antibiotics. Therefore, the development of vaccines to prevent infections with these bacteria is an urgent medical need. While the immunological activity of lipopolysaccharide O-antigens in P. aeruginosais well-known, the specific protective epitopes remain unidentified. Herein, we present the first chemical synthesis of highly functionalized aminoglycoside trisaccharide 1and its acetamido derivative 2found in the P. aeruginosaserotype O5 O-antigen. The synthesis of the trisaccharide targets is based on balancing the reactivity of disaccharide acceptors and monosaccharide donors. Glycosylations were analyzed by quantifying the reactivity of the hydroxyl group of the disaccharide acceptor using the orbital-weighted Fukui function and dual descriptor. The stereoselective formation of 1,2-cis-α-fucosylamine linkages was achieved through a combination of remote acyl participation and reagent modulation. The simultaneous SN2 substitution of azide groups at C2′ and C2″ enabled the efficient synthesis of 1,2-cis-β-linkages for both 2,3-diamino-D-mannuronic acids. Through a strategic orthogonal modification, the five amino groups on target trisaccharide 1were equipped with a rare acetamidino (Am) and four acetyl (Ac) groups. Glycan microarray analyses of sera from patients infected with P. aeruginosaindicated that trisaccharides 1and 2are key antigenic epitopes of the serotype O5 O-antigen. The acetamidino group is not an essential determinant of antibody binding. The β-D-ManpNAc3NAcA residue is a key motif for the antigenicity of serotype O5 O-antigen. These findings serve as a foundation for the development of glycoconjugate vaccines targeting P. aeruginosaserotype O5.

Published
2024
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7. N-Myc and the cyclin-dependent kinase inhibitors [p18.sup.Ink4c] and [p27.sup.Kip1] coordinately regulate cerebellar development

Author

Zindy, Frederique, Knoepfler, Paul S., Xie, Suqing, Sherr, Charles J., Eisenman, Robert N., and Roussel, Martine F.

Subjects
Cerebellum -- Growth, Cerebellum -- Genetic aspects, Phosphotransferases -- Physiological aspects, Company growth, Science and technology
Abstract

Conditional N-Myc deletion limits the proliferation of granule neuron progenitors (GNPs), perturbs foliation, and leads to reduced cerebellar mass. We show that c-Myc mRNA levels increase in N-Myc-null GNPs and that simultaneous deletion of both c- and N-Myc exacerbates defective cerebellar development. Moreover, N-Myc loss has been shown to trigger the precocious expression of two cyclin-dependent kinase inhibitors, Kip1 and Ink4c, in the cerebellar primordium. We now further demonstrate that the engineered disruption of the Kip1 and Ink4c genes in N-Myc-null cerebella partially rescues GNP cell proliferation and cerebellar foliation. These results provide definitive genetic evidence that expression of N-Myc and concomitant down-regulation of Ink4c and Kip1 contribute to the proper development of the cerebellum. Myc | N-Myc deficiency | cerebellum | granule neuron progenitor | cell cycle inhibitor

Published
2006

9. Principles, Detections and Applications of Cosmological Gravitational Waves

Author

Zhao Sirou, Xie Suqing, and Chen Hanhao

Subjects
Physics, Physics::General Physics, General Relativity and Quantum Cosmology, History, Gravitational wave, Astrophysics::High Energy Astrophysical Phenomena, Astronomy, Computer Science Applications, Education
Abstract

Contemporarily, the gravitational wave has played an important role in the field of astronomy. A systematic review of the gravitational wave is presented in this paper. Primarily, the principle of gravitational waves is introduced in both Newton and Einstein’s concept of gravity. Besides, we deduced the solution of Einstein’s field equation. Moreover, we described the detection of gravitational waves with the mechanisms of the two gravitational wave detectors LIGO and Virgo Finally, the applications of gravitational waves are discussed in detecting black hole mergers, neutron star mergers, gamma-ray burst, and core-Collapse Supernova explosion. The gravitational wave could contribute to more scientific findings in the future and solve more mysteries of the Universe.

Published
2021
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13. Experimental study on pancreatic cancer gene therapy using the HSV-TK gene mediated by a retroviral vector

Author

Li Zhaoshen, Xu Guoming, Xie Suqing, and Cao Guang-wen

Subjects
Retrovirus, Nude mouse, HSV-Tk Gene, Thymidine kinase, viruses, Genetic enhancement, Gastroenterology, Transfection, Biology, biology.organism_classification, Molecular biology, Long terminal repeat, Viral vector
Abstract

OBJECTIVE: To study the value of the herpes simplex virus type I thymidine kinase (HSV-TK) gene mediated by a retroviral vector in the treatment of human pancreatic cancer cell line 8988. METHODS: The HSV-TK gene was directionally cloned into a site following the SV40 promoter that was adjacent to the 5′ LTR (long terminal repeats) and the neomycin phosphotransferase gene in the retroviral vector pMNSM. This enabled the TK gene to integrate into the chromatin of the host cell. The recombinant plasmid pMNS-TK-M was then transfected into the retrovirus-packaging cell Pa317. Finally, the HSV-TK gene was transfected into pancreatic cancer cell line 8988 by the recombinant retrovirus after selection with G418. RESULTS: The HSV-TK gene was stably integrated into the host cell and its expression confirmed by Southern blotting and drug-sensitivity tests. In vitro studies showed that the acyclovir (ACV) sensitivity level in the 8988/TK+ cells was higher than that of the parent cells. The cells that were transfected with the TK gene were significantly susceptible to ACV. In vivo studies in nude mice showed that intraperitoneal injection of ACV might postpone the formation of implanted tumors and produce a treatment effect on the tumors. CONCLUSIONS: This study demonstrated that the HSV-TK/ACV retroviral system could be used in vivo to treat pancreatic cancer.

Published
2000
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14. Abstract 3559: FKBP5 is an androgen-responsive gene in thyroid cancer cells.

Author

MacEwan, Melanie E., primary, Wong, Ken, additional, Swati, Ismatun, additional, Xie, Suqing, additional, Rasul, Mohammad, additional, Ardacheva, Olena, additional, Buchsbaum, Joseph, additional, Shin, Edward, additional, Moscatello, Augustine, additional, Schantz, Stimson, additional, Tiwari, Raj K., additional, and Geliebter, Jan, additional

Published
2013
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15. Arachidonate 5 lipoxygenase expression in papillary thyroid carcinoma promotes invasion via MMP-9 induction

Author

Kummer, Nicolas T., primary, Nowicki, Theodore S., additional, Azzi, Jean P., additional, Reyes, Ismael, additional, Iacob, Codrin, additional, Xie, Suqing, additional, Swati, Ismatun, additional, Darzynkiewicz, Zbigniew, additional, Gotlinger, Katherine H., additional, Suslina, Nina, additional, Schantz, Stimson, additional, Tiwari, Raj K., additional, and Geliebter, Jan, additional

Published
2012
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18. Investigation of the Candidate Tumor Suppressor Gene prk in Prostate Cancer

Author

NEW YORK MEDICAL COLL VALHALLA, Dai, Wei, Wang, Qi, Xie, Suqing, NEW YORK MEDICAL COLL VALHALLA, Dai, Wei, Wang, Qi, and Xie, Suqing

Abstract

On the basis of the observations that the PLK3 gene is down-regulated or mutated in certain prostate tumor cell lines, as well as other tumors (5;8;9), we hypothesize that PLK3 is a tumor suppressor gene whose inactivation is an important event in the development of prostate cancer. We have proposed to characterize the genetic and epigenetic basis of aberrant PLK3 transcripts detected in prostate cancer, their prevalence and possible clinical relevance, to test the functional ability of PLK3 as a tumor suppressor gene, and to investigate the physiologic function of PLK3 by identification of proteins that interact with PLK3., The original document contains color images. All DTIC reproductions will be in black and white.

Published
2002

21. The tumor suppressors Ink4c and p53 collaborate independently with Patched to suppress medulloblastoma formation

Author

Uziel, Tamar, primary, Zindy, Frederique, additional, Xie, Suqing, additional, Lee, Youngsoo, additional, Forget, Antoine, additional, Magdaleno, Susan, additional, Rehg, Jerold E., additional, Calabrese, Christopher, additional, Solecki, David, additional, Eberhart, Charles G., additional, Sherr, Sarah E., additional, Plimmer, Sarah, additional, Clifford, Steven C., additional, Hatten, Mary E., additional, McKinnon, Peter J., additional, Gilbertson, Richard J., additional, Curran, Tom, additional, Sherr, Charles J., additional, and Roussel, Martine F., additional

Published
2005
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30. BUBR1deficiency results in abnormal megakaryopoiesis

Author

Wang, Qi, Liu, Tongyi, Fang, Yuqiang, Xie, Suqing, Huang, Xuan, Mahmood, Radma, Ramaswamy, Gita, Sakamoto, Kathleen M., Darzynkiewicz, Zbigniew, Xu, Ming, and Dai, Wei

Abstract

The physiologic function of BUBR1, a key component of the spindle checkpoint, was examined by generating BUBR1-mutant mice. BUBR1–/–embryos failed to survive beyond day 8.5 in utero as a result of extensive apoptosis. Whereas BUBR1+/–blastocysts grew relatively normally in vitro, BUBR1–/–blastocysts exhibited impaired proliferation and atrophied. Adult BUBR1+/–mice manifested splenomegaly and abnormal megakaryopoiesis. BUBR1haploinsufficiency resulted in an increase in the number of splenic megakaryocytes, which was correlated with an increase in megakaryocytic, but a decrease in erythroid, progenitors in bone marrow cells. RNA interference–mediated down-regulation of BUBR1 also caused an increase in polyploidy formation in murine embryonic fibroblast cells and enhanced megakaryopoiesis in bone marrow progenitor cells. However, enhanced megakaryopoiesis in BUBR1+/–mice was not correlated with a significant increase in platelets in peripheral blood, which was at least partly due to a defect in the formation of proplatelet-producing megakaryocytes. Together, these results indicate that BUBR1is essential for early embryonic development and normal hematopoiesis.

Published
2004
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32. Further characterization of human DNA polymerase delta interacting protein 38.

Author

Xie B, Li H, Wang Q, Xie S, Rahmeh A, Dai W, and Lee MY

Subjects
Cell Line, Cell Line, Tumor, Cell Nucleus metabolism, Cytoplasm metabolism, DNA Polymerase III metabolism, DNA Repair, DNA Replication, DNA-Directed DNA Polymerase metabolism, Dose-Response Relationship, Drug, Electrophoresis, Polyacrylamide Gel, Glutathione Transferase metabolism, HeLa Cells, Humans, Immunohistochemistry, Microscopy, Fluorescence, Mitochondria metabolism, Nuclear Proteins metabolism, Oncogene Proteins, Viral metabolism, Papillomavirus E7 Proteins, Peptides chemistry, Protein Biosynthesis, Protein Structure, Tertiary, Recombinant Fusion Proteins metabolism, Subcellular Fractions metabolism, Transfection, DNA Polymerase III chemistry, Nuclear Proteins physiology
Abstract

Polymerase delta interacting protein 38 (PDIP38) was identified as a human DNA polymerase (pol) delta interacting protein through a direct interaction with p50, the small subunit of human pol delta. PDIP38 was also found to interact with proliferating cell nuclear antigen, which suggested that it might play a role in vivo in the processes of DNA replication and DNA repair in the nucleus. In order to characterize further this novel protein, we have examined its subcellular localization by the use of immunochemical and cellular fractionation techniques. These studies show that PDIP38 is a novel mitochondrial protein and is localized mainly to the mitochondria. PDIP38 was shown to possess a functional mitochondrial targeting sequence that is located within the first 35 N-terminal amino acid residues. The mature PDIP38 protein is about 50 amino acid residues smaller than the full-length precursor PDIP38 protein, consistent with it being processed by cleavage of the mitochondrial targeting sequence during entry into the mitochondria. His-tagged mature PDIP38 inhibited pol delta activity in vitro and interacted with human papillomavirus 16 E7 oncoprotein, suggesting that PDIP38 might play a role in the pol delta-mediated viral DNA replication. Although the localization of PDIP38 to the mitochondria suggests that it serves functions within the mitochondria, we cannot eliminate the possibility that it may be involved in pol delta-mediated DNA replication or DNA repair under certain conditions such as viral infection.

Published
2005
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33. Differential expression of the mannose 6-phosphate/ insulin-like growth factor-II receptor in human breast cancer cell lines of different invasive potential.

Author

Xie S and Kang JX

Subjects
3' Untranslated Regions genetics, Female, Humans, Mutation, RNA, Messenger genetics, RNA, Messenger metabolism, Receptor, IGF Type 2 genetics, Sequence Analysis, DNA, Tumor Cells, Cultured, Breast Neoplasms metabolism, Breast Neoplasms pathology, Cathepsin D metabolism, Enzyme Precursors metabolism, Receptor, IGF Type 2 metabolism
Abstract

Background: Hypersecretion of the precursor of the lysosomal protease cathepsin D (procathepsin D) has been implicated in the invasive phenotype of human breast cancer. However, the mechanism of the abnormal secretion of procathepsin D remains unclear. Since the mannose 6-phosphate/insulin-like growth factor II receptor (M6P/IGF2R) plays a central role in the intracellular transport and endocytosis of M6P-containing lysosomal enzymes, a deficiency in functional M6P/IGF2R may underlie the hypersecretion of procathepsin D in invasive tumors., Material/methods: In the present study, we compared the profiles of cathepsin D and the M6P/IGF2R between the highly invasive MDA-MB-231 and the non-invasive MCF-7 cell lines., Results: MDA-MB-231cells were confirmed to secrete a much larger proportion of procathepsin D into the medium than MCF-7 cells. Addition of M6P to the culture medium significantly altered the secretion of procathepsin D by MCF-7 cells, but had little effect on cathepsin D distribution in MDA-MB-231cells. Both the M6P-binding capacity and the endocytosis of exogenous M6P-bearing proteins in MDA-MB-231 cells were far less than those in MCF-7 cells. mRNA analysis indicated that the levels of the M6P/IGF2R mRNA in MDA-MB-231 cells were not lower but were even higher than that in MCF-7 cells. Sequence analysis indicated a difference in the 3'-untranslated region of M6P/IGF2R between the two cell lines, but no mutation in the M6P-binding domain of the receptor., Conclusions: The results suggest that a potential defect in a post-transcriptional process (e.g., translation) may exist during synthesis of the M6P/IGF2R in MDA-MB-231cells, leading to failure to express sufficient functional M6P/IGF2R and thereby resulting in the hypersecretion of procathepsin D.

Published
2002

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