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Objective To investigate the gene carrying rate, mutation spectrum and frequency of thalassemia in the childbearing age population of Dai nationality in Yingjiang County, Dehong Prefecture, Yunnan Province, China and to provide a theoretical basis for strategy maiming for the prevention and control of thassemia birth defects. Methods Totally 400 cases of childbearing age population of Dai nationality in Yingjiang County were subjected to routine blood testing, capillary electrophoresis, and next generation sequencing. Results Among the 400 cases 219(54.75%) thalassemia gene carriers were found. There were 133(33.25%) carriers having α-thalassaemia gene. The most common genotype was -α3.7, followed by--SEA and -α4.2. There were 47(11.75%) carriers of β-thalassemia gene, and the mutation type CD26 (G＞A) was the most common type. There were 39(9.75%, 39/400) carriers of composite α and β thalassemia. According to comparative analysis of the hematological characteristics of various α-thalassemias, mean corpsular hemoglobin(MCH) of --SEA and Hb A2 of αCSα were relatively low. According to comparative analysis of the hematological characteristics of β-thalassemia, mean corpusular volume(MCV) of CD17 (A＞T) was relatively low. Conclusions The carrying rate of thalassaemia in Yingjiang County is 54.75%, indicating it is a high incidence area of thalassaemia. The most common types are -α3.7 and CD26 (G＞A). The results reported here may provide evidence for genetic counseling in areas with high incidence of thalassaemia.

The study of facile‐synthesis and low‐cost X‐ray scintillators with high light yield, low detection limit and high X‐ray imaging resolution plays a vital role in medical and industrial imaging fields. However, the optimal balance between X‐ray absorption, decay lifetime and excitonic utilization efficiency of scintillators to achieve high‐resolution imaging is extremely difficult due to the inherent contradiction. Here two thermally activated delayed fluorescence (TADF)‐actived coinage‐metal clusters M6S6L6 (M=Ag or Cu) were synthesized by simple solvothermal reaction, where the cooperation of heavy atom‐rich character and TADF mechanism supports strong X‐ray absorption and rapid luminescent collection of excitons. Excitingly, Ag6S6L6 (SC‐Ag) displays a high photoluminescence quantum yield of 91.6 % and scintillating light yield of 17420 photons MeV−1, as well as a low detection limit of 208.65 nGy s−1 that is 26 times lower than the medical standard (5.5 μGy s−1). More importantly, a high X‐ray imaging resolution of 16 lp/mm based on SC‐Ag screen is demonstrated. Besides, rigid core skeleton reinforced by metallophilicity endows clusters M6S6L6 strong resistance to humidity and radiation. This work provides a new view for the design of efficient scintillators and opens the research door for silver clusters in scintillation application. [ABSTRACT FROM AUTHOR]

Aimed to evaluate the contents of 10 heterocyclic aromatic amines (HAs) in two batches of halogen soups of stewed chicken legs randomly selected from three individual market stalls by using ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) . The results showed that the total contents of detected 8 HAs in halogen soups of stewed chicken legs from three individual market stalls reached 35. 626 ng/g. In that, both Harman and Norharman accounted for 41% ~77% of the total HAs. Thus, it was imperative to recommend the producers to strictly control the stewing process and reduce the recycle numbers of halogen soups. [ABSTRACT FROM AUTHOR]

Background: Patients with systemic sclerosis (SSc) are known to have higher incidence of atherosclerosis (AS). Mitochondrial injuries in SSc can cause endothelial dysfunction, leading to AS; thus, mitochondria appear to be hubs linking SSc to AS. This study aimed to identify the mitochondria-related biomarkers of SSc and AS., Methods: We identified common differentially expressed genes (DEGs) in the SSc (GSE58095) and AS (GSE100927) datasets of the Gene Expression Omnibus (GEO) database. Considering the intersection between genes with identical expression trends and mitochondrial genes, we used the least absolute shrinkage and selection operator (LASSO) as well as random forest (RF) algorithms to identify four mitochondria-related hub genes. Diagnostic nomograms were then constructed to predict the likelihood of SSc and AS. Next, we used the CIBERSORT algorithm to evaluate immune infiltration in both disorders, predicted the transcription factors for the hub genes, and validated these genes for the two datasets., Results: A total of 112 genes and 13 mitochondria-related genes were identified; these genes were then significantly enriched for macrophage differentiation, collagen-containing extracellular matrix, collagen binding, antigen processing and presentation, leukocyte transendothelial migration, and apoptosis. Four mitochondria-related hub DEGs (IFI6, FSCN1, GAL, and SGCA) were also identified. The nomograms showed good diagnostic values for GSE58095 (area under the curve (AUC) = 0.903) and GSE100927 (AUC = 0.904). Further, memory B cells, γδT cells, M0 macrophages, and activated mast cells were significantly higher in AS, while the resting memory CD4 + T cells were lower and M1 macrophages were higher in SSc; all of these were closely linked to multiple immune cells. Gene set enrichment analysis (GSEA) showed that IFI6 and FSCN1 were involved in immune-related pathways in both AS and SSc; GAL and SGCA are related to mitochondrial metabolism pathways in both SSc and AS. Twenty transcription factors (TFs) were predicted, where two TFs, namely BRCA1 and PPARγ, were highly expressed in both SSc and AS., Conclusion: Four mitochondria-related biomarkers were identified in both SSc and AS, which have high diagnostic value and are associated with immune cell infiltration in both disorders. Hence, this study provides new insights into the pathological mechanisms underlying SSc and AS. The specific roles and action mechanisms of these genes require further clinical validation in SSc patients with AS., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Wang, Lyu, Qin and Xie.)

Two examples of efficient cathode-ray scintillation coordination polymers with good stability at high voltage were prepared by conjugating luminescent groups with d 10 metal ions. The synergistic effect of inorganic metal and organic ligand suppresses the self-quenching of the conjugated luminescent groups and enhances the scintillation performance. This work provides new ideas for the design of new field-emission displays and cathode-ray scintillation materials.