Search

Your search keyword '"Xie, Liangkun"' showing total 10 results
Start Over Author "Xie, Liangkun"
10 results on '"Xie, Liangkun"'

4. miR‐148a‐3p regulates proliferation and apoptosis of idiopathic gingival fibroma by targeting NPTX1.

Author

Sun, Yuyang, Xie, Liangkun, Ren, Xiaobin, Ran, Liquan, He, Hongbing, Kong, Fanying, Yang, Shuran, and Zhang, Mingzhu

Subjects
*PROTEINS, *HYPERPLASIA, *FLOW cytometry, *COMPUTER-assisted molecular modeling, *RESEARCH funding, *T-test (Statistics), *MICRORNA, *CELL proliferation, *APOPTOSIS, *GINGIVA, *REVERSE transcriptase polymerase chain reaction, *CELL cycle, *DESCRIPTIVE statistics, *FIBROBLASTS, *CYTOMETRY, *WESTERN immunoblotting, *GENE expression profiling, *ANALYSIS of variance, *GINGIVAL hyperplasia, *TUMORS, *DATA analysis software, *COMPARATIVE studies, *ORAL health, *DISEASE progression, *SEQUENCE analysis
Abstract

Objective: Idiopathic gingival fibromatosis (IGF) is a rare heterogeneous disease that results in the progressive and diffuse hyperplasia of gingival tissues. MicroRNAs are implicated in the development and progression of various tumors. The present study aimed to explore the potential roles and mechanisms of miR‐148a‐3p in IGF. Methods: Gingival fibroblasts (GFs) were transfected with miR‐148a‐3p mimics, miR‐148a‐3p inhibitors, or siNPTX1, and then, the proliferation and apoptosis of GFs and the expression of related genes were evaluated using Cell Counting Kit‐8 assays, 5‐ethynyl‐2′‐deoxyuridine assays, flow cytometry, reverse transcription‐quantitative polymerase chain reaction, and western blot analysis, respectively. Results: miR‐148a‐3p was highly expressed in GFs of IGF (IGF‐GFs) as compared with normal GFs (N‐GFs). Overexpression of miR‐148a‐3p promoted the proliferation and inhibited the apoptosis of N‐GFs, whereas downregulation of miR‐148a‐3p had the opposite effect in IGF‐GFs. Knockdown of NPTX1 reversed miR‐148a‐3p‐mediated effects in IGF‐GFs. Dual‐luciferase reporter assay confirmed that NPTX1 is a direct target of miR‐148a‐3p. Conclusion: These findings identify that miR‐148a‐3p could regulate cell proliferation and apoptosis by targeting NPTX1, providing new insights for the further study of the molecular mechanism and treatment of IGF. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

6. Inhaled RNA drugs to treat lung diseases:Disease-related cells and nano–bio interactions

Author

Zhang, Mengjun, Lu, Haoyu, Xie, Liangkun, Liu, Xulu, Cun, Dongmei, Yang, Mingshi, Zhang, Mengjun, Lu, Haoyu, Xie, Liangkun, Liu, Xulu, Cun, Dongmei, and Yang, Mingshi

Abstract

In recent years, RNA-based therapies have gained much attention as biomedicines due to their remarkable therapeutic effects with high specificity and potency. Lung diseases offer a variety of currently undruggable but attractive targets that could potentially be treated with RNA drugs. Inhaled RNA drugs for the treatment of lung diseases, including asthma, chronic obstructive pulmonary disease, cystic fibrosis, and acute respiratory distress syndrome, have attracted more and more attention. A variety of novel nanoformulations have been designed and attempted for the delivery of RNA drugs to the lung via inhalation. However, the delivery of RNA drugs via inhalation poses several challenges. It includes protection of the stability of RNA molecules, overcoming biological barriers such as mucus and cell membrane to the delivery of RNA molecules to the targeted cytoplasm, escaping endosomal entrapment, and circumventing unwanted immune response etc. To address these challenges, ongoing researches focus on developing innovative nanoparticles to enhance the stability of RNA molecules, improve cellular targeting, enhance cellular uptake and endosomal escape to achieve precise delivery of RNA drugs to the intended lung cells while avoiding unwanted nano–bio interactions and off-target effects. The present review first addresses the pathologic hallmarks of different lung diseases, disease-related cell types in the lung, and promising therapeutic targets in these lung cells. Subsequently we highlight the importance of the nano–bio interactions in the lung that need to be addressed to realize disease-related cell-specific delivery of inhaled RNA drugs. This is followed by a review on the physical and chemical characteristics of inhaled nanoformulations that influence the nano–bio interactions with a focus on surface functionalization. Finally, the challenges in the development of inhaled nanomedicines and some key aspects that need to be considered in the development of futu

Published
2023

10. Exosomes derived from idiopathic gingival fibroma fibroblasts regulate gingival fibroblast proliferation and apoptosis.

Author

Yin, Shunhui, Jia, Fengmei, Ran, Liquan, Xie, Liangkun, Wu, Zhiyao, Zhan, Yeming, Zhang, Yan, and Zhang, Mingzhu

Subjects
GINGIVAL neoplasms, FLOW cytometry, REVERSE transcriptase polymerase chain reaction, EXOSOMES, FIBROBLASTS, IMMUNOHISTOCHEMISTRY, ONCOGENES, WESTERN immunoblotting, APOPTOSIS, ELECTRON microscopy, CELL proliferation, TUMOR markers, POLYMERASE chain reaction, CONNECTIVE tissue tumors
Abstract

Objective: Exosomes have been proved to play an essential role in intercellular information transmission. However, few researches focused on exosomes derived from gingival fibroblasts (GFs) of IGF. The aim of this study is to investigate the effect of exosomes derived from GFs of IGF (IGF‐GFs) on the proliferation and apoptosis of normal gingival fibroblasts (N‐GFs). Methods: Gingival fibroblasts were cultured and identified using immunocytochemistry. Exosomes were isolated with exosomes extraction kit and characterized by transmission electron microscopy (TEM) and flow cytometry. PKH67 labeling was further used to trace the intracellular distribution of the exosomes. And MTS assay was used to test the effective concentration and time course of IGF‐GFs‐derived exosomes. Furthermore, the expression of PCNA, Ki67, Bcl‐2, and Bax in N‐GFs was analyzed by qRT‐PCR and Western blot. Results: Exosomes were isolated from IGF‐GFs; the identification of exosomes and gingival fibroblasts was successfully finished. Moreover, we found that N‐GFs co‐cultured with exosomes showed a great increase in PCNA and Bcl‐2 levels, and a moderate increase in Ki67 levels. By contrast, the levels of Bax were significantly reduced. Conclusion: These results suggest that exosomes derived from idiopathic gingival fibroma fibroblasts are involved in the regulation of gingival fibroblast proliferation and apoptosis. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results