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This study evaluated the effects of feeding a microbial-derived antioxidant diet (MA) for 56 days on the growth performance of Eriocheir sinensis, as well as on digestive and metabolic enzyme activity and antioxidant capacity under ammonia stress (400 mg/L ammonia nitrogen for 48 h). The weight gain at 28d and the specific growth rate at 56ds for crabs in the MA group were significantly higher than those in the control diet (C) group (p

The Chinese mitten crab (Eriocheir sinensis, CMC) is of significant economic importance to China's aquatic industry. However, the prevalence of Planktothricoides-dominated cyanobacterial blooms (CBs) pose a substantial threat to this aquaculture sector. Despite their impact, there is limited understanding of how these CBs affect the digestive systems of CMCs. This study aims to investigate the effects of CBs dominated by Planktothricoides on CMC growth, intestinal bacterial communities, metabolism, and digestive enzyme activity. Our findings indicate that CBs dominated by Planktothricoides impede CMC growth and significantly alter immune and digestive enzyme activities. Notably, a decline in the richness and Shannon diversity of the intestinal bacterial community were observed, along with shifts in its structure and composition. The abundance of Epsilonbacteraeota, a group containing various conditional pathogens, reached 21.40 % at the family level in the CB group. A total of 26 bacteria were identified as potential pathogens in the CMC intestine. While immune activities demonstrated a strong correlation with potential pathogens, digestive enzyme activities were less correlated. Furthermore, CBs significantly impacted the metabolic pathways in the CMC intestine, leading to a marked reduction in several metabolites involved in lipid metabolism. In conclusion, this study focuses on the impact of CBs in natural environments on the digestive system of CMCs. It fills the knowledge gap regarding the actual negative effects of CBs on CMCs. Urgent interventions are warranted to support the prevention and control of CBs in CMC culture.

Agonistic behavior is a common behavior among agonistic Chinese mitten crabs (Eriocheir sinensis). Such behavior often leads to limb loss or physical impairment, and significantly affects the survival, growth, and quality of the crabs, and even the yield and economic value for E. sinensis. Agonistic behavior often occurs in agonistic crabs, which is closely related to personality traits and interactive behavior of animals. E. sinensis has personality traits such as boldness, aggression, and exploration as evidenced by the partition-crossing experiment, mirror experiment, and shelter experiment. Agonistic crabs were identified as individuals with boldness, high aggression, and high exploration. The interactive behavior spectrum of E. sinensis was first obtained, consisting of 15 behaviors. This spectrum described and summarized all interactive behaviors of E. sinensis during fights. The interactive behavior characteristics of agonistic crabs were identified as darting, intimidating, grabbing, pushing, stretching, and visitation. These results lay a theoretical basis for in-depth behavioral research on E. sinensis in the future. The technique of identifying agonistic crabs by personality traits and interactive behaviors not only allows for the elimination of agonistic crabs from the aquaculture process and the reduction in negative impacts caused by aggressive crabs, but also allows for the breeding of non-agonistic crabs and the further reduction in the economic losses caused by fighting behaviors.

Abstract Breaking reciprocity in the microwave frequency range is strongly desirable in the development of modern electronic systems, as it enables nonreciprocal wave absorbing, nonreciprocal beam steering, frequency conversion, and protection of sensitive devices from high energy sources. However, the typical approaches involve bulky biasing magnets or complex spatial-temporal modulations. As such, resorting to lightweight and all-passive platforms would result in optimal configurations and efficient integration. Starting from a circuit model, we theoretically demonstrate the nonreciprocal behavior on a transmission line building block creating a strong field asymmetry with a switchable matching stub to enable two distinct working states. After translating to an electromagnetic model, this concept is first proved by simulation and then experimentally verified on a microstrip-line-based diode-integrated metasurface showing nonreciprocal transmission. This printed circuit board design is expected to find various applications in electromagnetic protecting layers, communication systems, microwave isolators and circulators.

Abstract Manipulating the electromagnetic (EM) scattering behavior from an arbitrary surface dynamically on arbitrary design goals is an ultimate ambition for many EM stealth and communication problems, yet it is nearly impossible to accomplish with conventional analysis and optimization techniques. Here we present a reconfigurable conformal metasurface prototype as well as a workflow that enables it to respond to multiple design targets on the reflection pattern with extremely low on-site computing power and time. The metasurface is driven by a sequential tandem neural network which is pre-trained using actual experimental data, avoiding any possible errors that may arise from calculation, simulation, or manufacturing tolerances. This platform empowers the surface to operate accurately in a complex environment including varying incident angle and operating frequency, or even with other scatterers present close to the surface. The proposed data-driven approach requires minimum amount of prior knowledge and human effort yet provides maximized versatility on the reflection control, stepping towards the end form of intelligent tunable EM surfaces.

Parathyroid gland transplantation into the sternocleidomastoid muscle is effective, but it is not possible to confirm transplant survival with this method. In this study, we evaluated parathyroid autotransplantation into the brachioradialis muscle and its survival rate. Objectives: To evaluate autologous parathyroid gland left forearm brachioradial muscle transplantation and its survival rate. Summary background data: The most commonly used transplantation site is the sternocleidomastoid muscle, but transplant survival cannot be confirmed using this method. Autologous parathyroid gland left forearm brachioradial muscle transplantation solves this problem, and we evaluate the transplant survival using this method. Methods: We followed-up patients who underwent thyroidectomy and autologous parathyroid left forearm brachioradial muscle transplantation in our center from September 2013 to January 2018. The last follow-up date was January 2021; all enrolled patients underwent at least 3 years of follow-up. We calculated the transplant survival rate at several time points. Results: We evaluated 238 transplanted cases, for which the long-term survival rate was 85.7% (204/238), and the short-term survival rate was 86.1% (205/238). Sixty-five cases had two parathyroid glands transplanted into the left forearm brachioradialis muscle. The long-term survival rate was 92.3% (60/65), and the short-term survival rate was 95.4% (62/65). Conclusions: Autologous parathyroid gland left brachioradialis transplantation is a reliable, measurable method with good survival rate, and we recommend this method for consideration for transplanting parathyroid glands in thyroidectomy.

L-tryptophan (L-TRP) is an essential amino acid for the normal growth of crustaceans. As a nutritional supplement and antioxidant, L-TRP has the function of immune and antioxidant capacity regulation. From July to November, the effects of L-TRP on the immunity, antioxidant capacity and intestinal microflora of the Chinese mitten crab (Eriocheir sinensis) in pond culture were investigated. After feeding an L-TRP diet for 30 (named as August), 60 (named as September) and 106 (named as November) days, respectively, the activities of the immune and antioxidant enzymes in the hepatopancreas and hemolymph were evaluated, and the intestinal microbiota were profiled via high-throughput Illumina sequencing. The results showed that supplementation of L-TRP significantly increased the activities of AKP in the hepatopancreas in September, and significantly increased the activities of ACP in the hepatopancreas in August and September, and the hemolymph’s ACP activities also significantly increased in August and November (p < 0.05). Similarly, the activities of SOD, AOC and POD in the hepatopancreas significantly increased in September and November (p < 0.05) after feeding the L-TRP diet; meanwhile, the activities of SOD and AOC in the hemolymph also significantly increased in August (p < 0.05). However, in August, the L-TRP diet resulted in a significant increase in MDA activity in the hepatopancreas and hemolymph (p < 0.05). In addition, the results of the intestinal microbiota analysis showed that Firmicutes, Bacteroidetes and Proteobacteria were the dominant phyla in August, September and November, and Patescibacteria was the dominant phylum in September and November. After feeding the L-TRP diet, the richness of Cyanobacteria and Desulfobacterota significantly increased in August (p < 0.05), and the richness of Actinobacteriota significantly decreased in September (p < 0.05). Moreover, the L-TRP supplementation significantly reduced the abundance of ZOR0006 in the Firmicutes in September (p < 0.05). In conclusion, dietary L-TRP could improve the immunity and antioxidant ability and impact the intestinal health of E. sinensis at the early stage of pond culturing. However, long-term feeding of an L-TRP diet might have no positive impact on the activities of the immune, antioxidant enzymes and intestinal microbiota.

Abstract Background Predicting early recurrence (ER) after radical therapy for HCC patients is critical for the decision of subsequent follow-up and treatment. Radiomic features derived from the medical imaging show great potential to predict prognosis. Here we aim to develop and validate a radiomics nomogram that could predict ER after curative ablation. Methods Total 184 HCC patients treated from August 2007 to August 2014 were included in the study and were divided into the training (n = 129) and validation(n = 55) cohorts randomly. The endpoint was recurrence free survival (RFS). A set of 647 radiomics features were extracted from the 3 phases contrast enhanced computed tomography (CECT) images. The minimum redundancy maximum relevance algorithm (MRMRA) was used for feature selection. The least absolute shrinkage and selection operator (LASSO) Cox regression model was used to build a radiomics signature. Recurrence prediction models were built using clinicopathological factors and radiomics signature, and a prognostic nomogram was developed and validated by calibration. Results Among the four radiomics models, the portal venous phase model obtained the best performance in the validation subgroup (C-index = 0.736 (95%CI:0.726–0.856)). When adding the clinicopathological factors to the models, the portal venous phase combined model also yielded the best predictive performance for training (C-index = 0.792(95%CI:0.727–0.857) and validation (C-index = 0.755(95%CI:0.651–0.860) subgroup. The combined model indicated a more distinct improvement of predictive power than the simple clinical model (ANOVA, P

The assertive growth of photovoltaics (PV) will occupy a lot of land resources. There is also a needed land resource to expand the culturing area of Eriocheir sinensis. The aquavoltaic systems offer a potential solution to integrate PV power and E. sinensis culturing. In this study, we cultured E. sinensis in an area of PV panels (PV group) and an area with no PV panels (control group), respectively. The results showed that the weight gain rate, body length, body width, and meat yield of male crabs in the PV group significantly increases. In addition, the moisture of muscles, hepatopancreas, and testes in the PV group has significantly increased, and the total lipids of the hepatopancreas and muscles in the PV group were significantly decreased. Moreover, the PV panels affected the content of eight amino acids in different tissues, including Met, Arg, Cys, Pro, Gly, Leu, Tyr, and His. In addition, several saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) had been significantly influenced by the PV panels. ∑MUFA, docosahexaenoic acid (DHA), ∑n-3 PUFA, ∑n-6 PUFA, and n-3/n-6 PUFA ratio (p < 0.05) in the PV group had significantly decreased. The results of tissue color showed that the L* value of ovaries was significantly higher than in the control group, and a* and b* values of hepatopancreas were significantly lower. In conclusion, PV panels could promote growth performance and amino acid nutrition of crabs. However, the PV panels had a few negative influences on the fatty acid composition and tissue color.

As a commercially important species, the Chinese mitten crab (Eriocheir sinensis) has been cultured for a long time in China. Agonistic behavior often causes limb disability and requires much energy, which is harmful to the growth and survival of crabs. In this paper, we divided crabs into a control group (control, no treatment) and an experimental group (fight, agonistic behavior after 1 h) and then collected the thoracic ganglia (TG) to extract RNA. Subsequently, we first used a deep sequencing approach to examine the transcripts of microRNAs (miRNAs) and messenger RNAs (mRNAs) in E. sinensis displaying agonistic behavior. According to the results, we found 29 significant differentially expressed miRNAs (DEMs) and 116 significant differentially expressed unigenes (DEGs). The DEMs esi-miR-199a-5p, esi-let-7d, esi-miR-200a, and esi-miR-200b might participate in the regulation of agonistic behavior by mediating neuroregulation and energy metabolism. Focusing on the transcripts of the mRNAs, the renin–angiotensin system (RAS) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway might be involved in the regulation of agonistic behavior through glucose metabolism as this pathway was significantly enriched with DEGs. Besides, an integrated analysis of the miRNA and mRNA profiles revealed that the retinoid X receptor (RXR) was also involved in visual signal transduction, which was important for agonistic behavior. In addition, four vital agonistic behavior-related metabolic pathways, including the cAMP signaling, MAPK, protein digestion and absorption, and fatty acid metabolism pathways, were significantly enriched with the predicted target unigenes. In conclusion, the findings of this study might provide important insight enhancing our understanding of the underlying molecular mechanisms of agonistic behavior in E. sinensis.

Monochamus alternatus alternatus is the major vector of pinewood nematode, Bursaphelenchus xylophilus, in Asia. The length of the complete mitochondria genome of M. alternatus alternatus was 15,880 bp with 21% GC content, including 39.7% A, 12.3% C, 8.7% G and 39.3% T. There were 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and one AT-rich region. This study provides a useful genetic information for subsequent study of the differences between M. alternatus subspecies.

In this study, the first complete mitochondrial genome of Myrmeleon formicarius was sequenced. The length of the complete mitochondrial genome of M. formicarius was 16,179 bp with 25.19% GC content. There were 13 protein-coding genes (CDS), 22 transfer RNA genes (tRNA), two ribosomal RNA genes (rRNA), and an AT-rich region. Phylogenetic analysis showed that M. formicarius was closely related to M. immanis. This study provides useful genetic information to better understand the genetic evolution of Myrmeleontidae.

The insecticidal Cry4Ba and Cry11Aa crystal proteins from Bacillus thuringiensis subsp. israelensis (Bti) are highly toxic to Ae. aegypti larvae. The glycosylphosphatidylinositol (GPI)-anchored APN was identified as an important membrane-bound receptor for multiple Cry toxins in numerous Lepidoptera, Coleoptera, and Diptera insects. However, there is no direct molecular evidence to link APN of Ae. aegypti to Bti toxicity in vivo. In this study, two Cry4Ba/Cry11Aa-binding Ae. aegypti GPI-APN isoforms (AeAPN1 and AeAPN2) were individually knocked-out using CRISPR/Cas9 mutagenesis, and the AeAPN1/AeAPN2 double-mutant homozygous strain was generated using the reverse genetics approach. ELISA assays showed that the high binding affinity of Cry4Ba and Cry11Aa protoxins to the midgut brush border membrane vesicles (BBMVs) from these APN knockouts was similar to the background from the wild-type (WT) strain. Likewise, the bioassay results showed that neither the single knockout of AeAPN1 or AeAPN2, nor the simultaneous disruption of AeAPN1 and AeAPN2 resulted in significant changes in susceptibility of Ae. aegypti larvae to Cry4Ba and Cry11Aa toxins. Accordingly, our results suggest that AeAPN1 and AeAPN2 may not mediate Bti Cry4Ba and Cry11Aa toxicity in Ae. aegypti larvae as their binding proteins.

Pathogens hitting the plant cell wall is the first impetus that triggers the phenylpropanoid pathway for plant defense. The phenylpropanoid pathway bifurcates into the production of an enormous array of compounds based on the few intermediates of the shikimate pathway in response to cell wall breaches by pathogens. The whole metabolomic pathway is a complex network regulated by multiple gene families and it exhibits refined regulatory mechanisms at the transcriptional, post-transcriptional, and post-translational levels. The pathway genes are involved in the production of anti-microbial compounds as well as signaling molecules. The engineering in the metabolic pathway has led to a new plant defense system of which various mechanisms have been proposed including salicylic acid and antimicrobial mediated compounds. In recent years, some key players like phenylalanine ammonia lyases (PALs) from the phenylpropanoid pathway are proposed to have broad spectrum disease resistance (BSR) without yield penalties. Now we have more evidence than ever, yet little understanding about the pathway-based genes that orchestrate rapid, coordinated induction of phenylpropanoid defenses in response to microbial attack. It is not astonishing that mutants of pathway regulator genes can show conflicting results. Therefore, precise engineering of the pathway is an interesting strategy to aim at profitably tailored plants. Here, this review portrays the current progress and challenges for phenylpropanoid pathway-based resistance from the current prospective to provide a deeper understanding.

In pond cultures of juvenile Eriocheir sinensis, limb autotomy stress seriously affects and restricts the quality and economic benefits of aquaculture. This study was designed to evaluate the effects of dietary supplementation of L-tryptophan on E. sinensis under the cheliped autotomy stress. In the present study, 252 crabs were divided into four groups: dietary L-trp supplementation with 0.28, 0.40, 0.53, and 0.70%, and their hematological immunity, antioxidant capacity, anti-stress, and antibacterial ability were evaluated after 14 days of using biochemical analysis, flow cytometry, and molecular biology techniques. First, we counted the mortality after 14 days of feeding and found that compared with other treatments, dietary supplementation of 0.53 and 0.70% L-trp significantly lowered the mortality of E. sinensis. Moreover, the total hemocyte count (THC), hemocyanin, and glutathione (GSH) content, and glutathione peroxidase (GSH-Px) activity significantly increased at 7 and 14 d with dietary supplementation of 0.53 and 0.70% L-trp, in contrast with the significant decrease in malondialdehyde (MDA) content at 14 d in the same dietary groups (P

As representatives of n-6 and n-3 fatty acids, many studies have analyzed the use of soybean oil and linseed oil rich in linoleic acid (18:2n-6, LA) and α-linolenic acid (18:3n-3, LNA) as better substitutes for fish oil. In aquatic animals, different dietary ratios of LA and LNA could have significant effects on growth, lipid metabolism, immune response, and reproduction. To assess the nutritive value of these two fatty acids in Chinese mitten crab (Eriocheir sinensis), we performed transcriptome analysis and label-free quantification proteomic analysis of the hepatopancreas from mitten crabs fed with LA or LNA diet. Parallel reaction monitoring was used to confirm the reliability of the proteomic analysis. A total of 186 proteins were differentially expressed with fold change ≥1.5 or ≤0.666. Among the 186 proteins, 116 were upregulated and 70 were downregulated in the LA than LNA. Most of these proteins participate in cellular process and metabolism process and have molecular functions such as binding and catalytic activity; the cellular component of these proteins are cell, cell part, membrane, and membrane part. A total of 18 proteins were identified to be related to lipid, carbohydrate, and protein metabolism, and they mainly participate in digestive enzyme activities, fatty acid transport, and glycolysis. Our results provide new insights for further investigation into the replacement of fish oil from mitten crabs with vegetable oils and enable us to better understand the different roles and nutrition value of LA and LNA in mitten crabs.

Melatonin has been identified in a variety of invertebrate species, but its function is not as well understood as in crustaceans. The effects of melatonin on hemolymph glucose levels and tissue carbohydrate metabolism in the Chinese mitten crab, Eriocheir sinensis, were fully investigated in this study. Moreover, whether the eyestalk (an important endocrine center in invertebrate species) involves in this process or not, also were clarified. Analysis revealed that eyestalk ablation, especially bilateral, caused a significant decrease in the hemolymph glucose level. Moreover, injection of melatonin induced hyperglycemia in a dose-dependent manner both in intact and ablated crabs. Based on the expression of CHH mRNA in the 10 different tissues, eyestalk, thoracic ganglion, intestinal tract and hemolymph were selected to estimate the effect of melatonin on the expression of CHH mRNA. Bilateral eyestalk ablation caused a significant increase in the expression of CHH mRNA in the thoracic ganglion, intestinal tract and hemolymph compared with the controls. In addition, injection of melatonin into intact or ablated crabs elevated the CHH mRNA level in the eyestalk, thoracic ganglion and intestinal tract tissues compared with controls. The hemolymph CHH mRNA after melatonin injection was elevated only in ablated crabs. Administration of melatonin resulted in a significant decrease in total carbohydrates and glycogen levels with an increase in phosphorylase activity levels in the hepatopancreas and muscle in intact and ablated crabs. Our findings demonstrated that melatonin can induce hyperglycemic effects in both intact and ablated crabs, suggesting that this effect is probably not mediated solely via eyestalk.

Despite identification of WRKY family genes in numerous plant species, a little is known about WRKY genes in watermelon, one of the most economically important fruit crops around the world. Here, we identified a total of 63 putative WRKY genes in watermelon and classified them into three major groups (I-III) and five subgroups (IIa-IIe) in group II. The structure analysis indicated that ClWRKYs with different WRKY domains or motifs may play different roles by regulating respective target genes. The expressions of ClWRKYs in different tissues indicate that they are involved in various tissue growth and development. Furthermore, the diverse responses of ClWRKYs to drought, salt, or cold stress suggest that they positively or negatively affect plant tolerance to various abiotic stresses. In addition, the altered expression patterns of ClWRKYs in response to phytohormones such as, ABA, SA, MeJA, and ETH, imply the occurrence of complex cross-talks between ClWRKYs and plant hormone signals in regulating plant physiological and biological processes. Taken together, our findings provide valuable clues to further explore the function and regulatory mechanisms of ClWRKY genes in watermelon growth, development, and adaption to environmental stresses.

Dopamine (DA) plays a modulatory role in numerous physiological processes such as light adaptation and food intake, and exerts these functions through DA receptors (DARs). This study presents, for the first time, isolation and characterization of the dopamine receptor 2(DA2 receptor) cDNA from the intestinal tissue of Eriocheir sinensis, an economically important freshwater aquaculture species in China. The DA2 receptor cDNA sequence, which was obtained by rapid amplification of cDNA ends, is 2369bp long, encode peptide of 589 amino acid, and is highly homologous to related sequences in crustaceans. Analysis of the deduced amino acid sequence and the structure of the DA2 indicated that this receptor is a member of the family of G protein-coupled receptors (GPCRs), as it contains seven transmembrane domains and other common signatures of GPCRs. RT-PCR showed that the expression of the DA2 receptor gene was distributed in various tissues, and high expression levels were observed in the cranial ganglia and the thoracic ganglia. Further study of the effect of photoperiod on DA2 expression showed that constant darkness induced a significant increase in DA2 expression in the cranial ganglia. Finally, analysis of DA2 receptor expression under different feeding statuses showed that there was significantly greater expression in the hepatopancreas and intestines after feeding than before feeding, but there were no differences in expression between the before feeding and during feeding periods in either tissue. Our results indicate that the DA2 receptor structurally belongs to the family of G protein-coupled receptors, and that the cranial ganglia are the main tissues in which the DA2 receptor participates in light adaptation during dark hours. In addition, the DA2 receptor in E. sinensis may be involved in the physiological regulation of the hepatopancreas and digestive tract after the ingestion of food. This study provides a foundation for further exploration of the light adaptation and digestive functions of the DA2 receptor in decapods.

The calcium-dependent protein kinase (CDPK) is a ser/thr protein kinase that plays vital roles in plant growth, development, and responses to multiple stresses. Despite an important member of the stress responsive gene family, little is known about the evolutionary history and expression patterns of CDPK genes in melon. Herein, a total of 18 CDPK genes and 7 CDPK-related protein kinases (CRK) genes were identified in the melon genome via bioinformatic analysis, which were unevenly distributed across eleven chromosomes with an apparent exception for chromosome 3. Comparative syntenic analysis between Cucumis melo L. and Arabidopsis thaliana revealed that 13 CmCDPKs and 19 AtCPKs existed in 20 corresponding syntenic blocks. In addition, based on gene structure and phylogenetic analyses, all CmCDPKs were divided into four groups (CDPK I-IV) and CmCRKs clustered into one group (CRK I). Interestingly, group CDPK IV was clearly distinct from the other three CDPK groups, but clustered with CRK I on the phylogenetic tree, implying their origination from a common ancestor. Furthermore, CmCDPKand CmCRK genes were differentially expressed in response to various stimuli, such as biotic stress (Podosphaera xanthii), abiotic stress (salt and cold), and hormone (abscisic acid) treatment. To our knowledge, this is the first report on CDPK and CRK gene families in melon, which provides a basic foundation for functional characterizations of CmCDPK and CmCRK genes in the future.

Background: Histopathological grading is a significant risk factor for postsurgical recurrence in hepatocellular carcinoma (HCC). Preoperative knowledge of histopathological grading could provide instructive guidance for individualized treatment decision-making in HCC management. Purpose: This study aims to develop and validate a newly proposed deep learning model to predict histopathological grading in HCC with improved accuracy. Methods: In this dual-centre study, we retrospectively enrolled 384 HCC patients with complete clinical, pathological and radiological data. Aiming to synthesize radiological information derived from both tumour parenchyma and peritumoral microenvironment regions, a modelling strategy based on a multi-scale and multi-region dense connected convolutional neural network (MSMR-DenseCNNs) was proposed to predict histopathological grading using preoperative contrast enhanced computed tomography (CT) images. Multiscale inputs were defined as three-scale enlargement of an original minimum bounding box in width and height by given pixels, which correspondingly contained more peritumoral analysis areas with the enlargement.Multi-region inputs were defined as three regions of interest (ROIs) including a squared ROI, a precisely delineated tumour ROI, and a peritumoral tissue ROI. The DenseCNN structure was designed to consist of a shallow feature extraction layer, dense block module, and transition and attention module. The proposed MSMRDenseCNN was pretrained by the ImageNet dataset to capture basic graphic characteristics from the images and was retrained by the collected retrospective CT images. The predictive ability of the MSMR-DenseCNN models on triphasic images was compared with a conventional radiomics model, radiological model and clinical model. Results: MSMR-DenseCNN applied to the delayed phase (DP) achieved the highest area under the curve (AUC) of 0.867 in the validation cohort for grading prediction, outperforming those on the arterial phase (AP) and portal venous phase (PVP). Fusion of the results on triphasic images did not increase the predictive ability, which underscored the role of DP for grading prediction. Compared with a single-scale and single-region network, the DP-phase based MSMR-DenseCNN model remarkably raised sensitivity from 67.4% to 75.5% with comparable specificity of 78.6%. MSMR-DenseCNN on DP defeated conventional radiomics, radiological and clinical models, where the AUCs were correspondingly 0.765, 0.695 and 0.612 in the validation cohort. Conclusions: The MSMR-DenseCNN modelling strategy increased the accuracy for preoperative prediction of grading in HCC, and enlightens similar radiological analysis pipelines in a variety of clinical scenarios in HCC management. [ABSTRACT FROM AUTHOR]