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Tumor immunotherapy occupies a pivotal position in the field of hematological malignancies. Chimeric antigen receptor (CAR) T-cell therapy has established a new therapeutic pattern for hematological immunotherapy and achieved satisfactory clinical results in the treatment of B-lineage hematological malignancies. However, CAR T-cell therapy has some limitations in the treatment of T-cell acute lymphoblastic leukemia because of the presence of CAR T-cell fratricide, tumor cell contamination, T-cell aplasia, and other clinically relevant problems. Therefore, the current major challenge is overcoming the existing bottlenecks to optimize CAR-T therapy and improve its efficacy against T-ALL while improving the prognosis of patients.

Abstract Chimeric antigen receptor (CAR) T cells show suboptimal efficacy in acute myeloid leukemia (AML). We find that CAR T cells exposed to myeloid leukemia show impaired activation and cytolytic function, accompanied by impaired antigen receptor downstream calcium, ZAP70, ERK, and C-JUN signaling, compared to those exposed to B-cell leukemia. These defects are caused in part by the high expression of CD155 by AML. Overexpressing C-JUN, but not other antigen receptor downstream components, maximally restores anti-tumor function. C-JUN overexpression increases costimulatory molecules and cytokines through reinvigoration of ERK or transcriptional activation, independent of anti-exhaustion. We conduct an open-label, non-randomized, single-arm, phase I trial of C-JUN-overexpressing CAR-T in AML (NCT04835519) with safety and efficacy as primary and secondary endpoints, respectively. Of the four patients treated, one has grade 4 (dose-limiting toxicity) and three have grade 1–2 cytokine release syndrome. Two patients have no detectable bone marrow blasts and one patient has blast reduction after treatment. Thus, overexpressing C-JUN endows CAR-T efficacy in AML.

Abstract Land‒atmosphere coupling intensifies the vulnerability of ecosystems in drylands. However, whether and how ecological restoration would modify the land‒atmosphere coupling across drylands remains unclear. To address these gaps, here we use structural equation model to separate two pathways of land‒atmosphere coupling: vegetation and soil moisture pathways, and investigate the effect of ecological restoration in China’s drylands on land‒atmosphere coupling. Analysis reveals that, land‒atmosphere coupling regulates approximately 30% of atmospheric drought, among which soil moisture pathway contributes twice as much as vegetation pathway. Vegetation greening mitigates atmospheric drought in areas where the aridity index ranges from 0.3 to 0.5, while soil drying exacerbates atmospheric drought in areas where the aridity index ranges from 0.5 to 0.65. The findings identify the optimal regions where ecological restoration helps alleviate the vulnerability of ecosystems under anthropogenic warming. Additionally, the proposed method enhances the understanding of how restored ecosystems contribute to mitigating atmospheric drought.

Land degradation from water erosion poses a significant threat to water security and ecosystem stability, driving global efforts in soil conservation. Quantitative assessment of soil conservation benefits—both on-site and off-site—is crucial for guiding effective conservation strategies. However, existing methodologies often fall short in quantifying the value of these combined benefits. Here, we present a comprehensive framework for quantifying soil conservation service flows in monetary terms, evaluating the effectiveness of both on-site and off-site measures. Applying this framework to the Yellow River Basin (YRB), we employ cost-avoidance algorithms related to soil fertility maintenance, dredging cost reduction, and mitigation of nonpoint source pollution. Our results reveal that while many areas contribute to both on-site and off-site benefits, over half of the YRB relies predominantly on off-site services. By strategically enhancing key regions—which constitute 30% of the basin—we demonstrate that the overall soil conservation service supply can increase by 64.2% over the multi-year average from 2001 to 2020 compared to a consideration of on-site only. These findings underscore the essential role of off-site services in fully understanding soil conservation needs, particularly in large river basins, and the identified priority areas can offer valuable insights for optimizing soil conservation efforts.

Nature's contributions to people (NCP) encompass both the beneficial and detrimental effects of living nature on human quality of life, including regulatory, material, and non-material contributions. Globally, vital NCPs have been deteriorating, accelerated by changes in both natural and anthropogenic drivers over recent decades. Despite the often inevitable trade-offs between NCPs due to their spatially and temporally uneven distributions, few studies have quantitatively assessed the impacts of different drivers on the spatial and temporal changes in multiple NCPs and their interrelationships. Here we evaluate the effects of precipitation, temperature, population, gross domestic product, vegetation restoration, and urban expansion on four key regulatory NCPs—habitat maintenance, climate regulation, water quantity regulation, and soil protection—in Nei Mongol at the county level. We observe increasing trends in climate regulation and soil protection from 2000 to 2019, contrasted with declining trends in habitat maintenance and water quantity regulation. We have identified the dominant positive and negative drivers influencing each NCP across individual counties, finding that natural drivers predominantly overpowered anthropogenic drivers. Furthermore, we discover significant spatial disparities in the trade-off or synergy relationships between NCPs across the counties. Our findings illustrate how the impacts of various drivers on NCPs and their interrelationships can be quantitatively evaluated, offering significant potential for application in various spatial scales. With an understanding of trade-offs and scale effects, these insights are expected to support and inform policymaking at both county and provincial levels.

Climate change and anthropogenic activities are reshaping dryland ecosystems globally at an unprecedented pace, jeopardizing their stability. The stability of these ecosystems is crucial for maintaining ecological balance and supporting local communities. Yet, the mechanisms governing their stability are poorly understood, largely due to the scarcity of comprehensive field data. Here we show the patterns of community temporal stability and its determinants across an aridity spectrum by integrating a transect survey across China's drylands with remote sensing. Our results revealed a U-shaped relationship between community temporal stability and aridity, with a pivotal shift occurring around an aridity level of 0.88. In less arid areas (aridity level below 0.88), enhanced precipitation and biodiversity were associated with increased community productivity and stability. Conversely, in more arid zones (aridity level above 0.88), elevated soil organic carbon and biodiversity were linked to greater fluctuations in community productivity and reduced stability. Our study identifies a critical aridity threshold that precipitates significant changes in community stability in China's drylands, underscoring the importance of distinct mechanisms driving ecosystem stability in varying aridity contexts. These insights are pivotal for developing informed ecosystem management and policy strategies tailored to the unique challenges of dryland conservation.

Abstract Background Chimeric antigen receptor (CAR) T cells have shown significant activity in B-lineage malignancies. However, their efficacy in myeloid leukemia has not been successful due to unclear molecular mechanisms. Methods We conducted in vitro and in vivo experiments to investigate whether myeloid leukemia cells directly induce CAR down-regulation. Furthermore, we designed a CD33 CARKR in which all lysines in the cytoplasmic domain of CAR were mutated to arginine and verified through in vitro experiments that it could reduce the down-regulation of surface CARs and enhance the killing ability. Transcriptome sequencing was performed on various AML and ALL cell lines and primary samples, and the galectin-1-specific inhibitory peptide (anginex) successfully rescued the killing defect and T-cell activation in in vitro assays. Results CAR down-regulation induced by myeloid leukemia cells under conditions of low effector-to-tumor ratio, which in turn impairs the cytotoxicity of CAR T cells. In contrast, lysosomal degradation or actin polymerization inhibitors can effectively alleviate CAR down-regulation and restore CAR T cell-mediated anti-tumor functions. In addition, this study identified galectin-1 as a critical factor used by myeloid leukemia cells to induce CAR down-regulation, resulting in impaired T-cell activation. Conclusion The discovery of the role of galectin-1 in cell surface CAR down-regulation provides important insights for developing strategies to restore anti-tumor functions.

Abstract In pneumonia, the deficient or delayed pathogen clearance can lead to pathogen proliferation and subsequent overactive immune responses, inducing acute lung injury (ALI). While screening human genome coding genes using our peripheral blood cell chemotactic platform, we unexpectedly find SLP adaptor and CSK interacting membrane protein (SCIMP), a protein with neutrophil chemotactic activity secreted during ALI. However, the specific role of SCIMP in ALI remains unclear. In this study, we investigate the secretion of SCIMP in exosomes (SCIMPexo) by macrophages after bacterial stimulation, both in vitro and in vivo. We observe a significant increase in the levels of SCIMPexo in bronchoalveolar lavage fluid and serum of pneumonia patients. We also find that bronchial perfusion with SCIMPexo or SCIMP N-terminal peptides increases the survival rate of the ALI model. This occurs due to the chemoattraction and activation of peripheral neutrophils dependent on formyl peptide receptor 1/2 (FPR1/2). Conversely, exosome suppressors and FPR1/2 antagonists decrease the survival rate in the lethal ALI model. Scimp-deficient and Fpr1/2-deficient mice also have lower survival rates and shorter survival times than wild-type mice. However, bronchial perfusion of SCIMP rescues Scimp-deficient mice but not Fpr1/2-deficient mice. Collectively, our findings suggest that the macrophage-SCIMP-FPRs-neutrophil axis plays a vital role in the innate immune process underlying ALI.

Abstract Rising CO2 is accelerating Earth warming and drying by altering the radiation budget (CO2 radiative effect), and it is commonly thought that future biodiversity will be greatly threatened. However, elevated CO2 also modifies plant physiology including photosynthesis rate and water use efficiency (CO2 physiological effect), the impacts of which have been usually ignored. By coupling machine learning techniques and Earth system models, this study considered both of the two effects and simulated terrestrial vertebrate biodiversity changes under moderate CO2 emission pathway. Our results show that the CO2 radiative effect positively impacts terrestrial vertebrate biodiversity at high latitudes but has a negative impact at low latitudes, whereas the physiological effect generally has a positive impact that peaks near the equator. Between 45°N and 45°S, 15% of areas with diminishing terrestrial vertebrate biodiversity due to radiative effect experience a reversal in trend when physiological effect is also considered; species migration pressure is also mitigated. Additionally, the protection priority rankings of 36 biodiversity hotspots need to be readjusted with the consideration of CO2 physiological effect, for which the impacts on terrestrial vertebrate biodiversity are important and varied greatly over space. Overall, our findings highlight the critical impacts of vegetation physiology on terrestrial vertebrate biodiversity in response to elevated atmospheric CO2, which can provide a reference for resource allocation and biodiversity conservation policymaking.

Abstract Endometriosis is a gynecological inflammatory disease that is linked with immune cells, specifically macrophages. IL-33 secreted from macrophages is known to accelerate the progression of endometriosis. The periodic and repeated bleeding that occurs in women with endometriosis leads to excess iron in the microenvironment that is conducive to ferroptosis, a process related to intracellular ROS production, lipid peroxidation and mitochondrial damage. It is suggested that eESCs may specifically be able to inhibit ferroptosis. However, it is currently unclear whether IL-33 directly regulates ferroptosis to influence the disease course in endometriosis. In this study, eESCs co-cultured with macrophages or stimulated with IL-33/ST2 were observed to have increased cell viability and migration. Additionally, IL-33/ST2 decreased intracellular iron levels and lipid peroxidation in eESCs exposed to erastin treatment. Furthermore, IL-33/ST2 treatment resulted in a notable upregulation in SLC7A11 expression in eESCs due to the downregulation of negative transcription factor ATF3, thereby suppressing ferroptosis. The P38/JNK pathway activated by IL-33/ST2 was also found to inhibit the transcription factor ATF3. Therefore, we concluded that IL-33/ST2 inhibits the ATF3-mediated reduction in SLC7A11 transcript levels via the P38/JNK pathway. The findings reveal that macrophage-derived IL-33 upregulates SLC7A11 in eESCs through the p38/JNK/ATF3 pathway, ultimately resulting in protection against ferroptosis in eESCs. Moreover, we conducted an experiment using endometriosis model mice that showed that a combination of IL-33-Ab and erastin treatment alleviated the disease, showing the promise of combining immunotherapy and ferroptosis therapy.

Abstract Prenylated and reverse-prenylated indolines are privileged scaffolds in numerous naturally occurring indole alkaloids with a broad spectrum of important biological properties. Development of straightforward and stereoselective methods to enable the synthesis of structurally diverse prenylated and reverse-prenylated indoline derivatives is highly desirable and challenging. In this context, the most direct approaches to achieve this goal generally rely on transition-metal-catalyzed dearomative allylic alkylation of electron-rich indoles. However, the electron-deficient indoles are much less explored, probably due to their diminished nucleophilicity. Herein, a photoredox-catalyzed tandem Giese radical addition/Ireland–Claisen rearrangement is disclosed. Diastereoselective dearomative prenylation and reverse-prenylation of electron-deficient indoles proceed smoothly under mild conditions. An array of tertiary α-silylamines as radical precursors is readily incorporated in 2,3-disubstituted indolines with high functional compatibility and excellent diastereoselectivity (>20:1 d.r.). The corresponding transformations of the secondary α-silylamines provide the biologically important lactam-fused indolines in one-pot synthesis. Subsequently, a plausible photoredox pathway is proposed based on control experiments. The preliminary bioactivity study reveals a potential anticancer property of these structurally appealing indolines.

The rapid advancement of modern technology has significantly driven progress in various IT-related activities, resulting in a substantial increase in internet penetration rates, particularly among college students. The utilization of the internet has become one of the most essential tools in our modern society. However, internet addiction (IA) has emerged as a serious concern, particularly among college students, adversely affecting academic performance and having significant psychological and psychiatric implications. The aim of the current study was to determine the impact of physical exercise, gender and academic year on IA among college students. In the present study, we investigated internet usage, engagement in sports activities, and academic performance among college students from Western, Middle, and Eastern regions of Chinese universities. It’s noteworthy that most of the respondents were freshmen. Our findings indicate that freshmen students were more susceptible to experiencing IA. Approximately 75% of students engaged in leisure sports activities, revealing an inverse correlation between sports activity and IA. This correlation aligns with the level of sports involvement, emphasizing the potential benefits of physical activity in mitigating IA. However, our study did not uncover any correlation between geographic location and the occurrence of IA, nor did it find differences between medical and non-medical students. Furthermore, our study revealed no significant variations in IA among students from different ethnic backgrounds. The underlying mechanism of IA is being currently determined. Our data suggest that physical exercise, gender, and academic year have a significant impact on IA among college students.

Abstract Background Donor-derived CD7-directed chimeric antigen receptor (CAR) T cells showed feasibility and early efficacy in patients with refractory or relapsed T-cell acute lymphoblastic leukemia (r/r T-ALL), in a previous phase I trial report, at a median follow-up of 6.3 months. Here we report long-term safety and activity of the therapy after a 2-year follow-up. Methods Participants received CD7-directed CAR T cells derived from prior stem cell transplantation (SCT) donors or from HLA-matched new donors after lymphodepletion. The target dose was 1 × 106 (± 30%) CAR T cells per kg of patient weight. The primary endpoint was safety with efficacy secondary. This report focuses on the long-term follow-up and discusses them in the context of previously reported early outcomes. Results Twenty participants were enrolled and received infusion with CD7 CAR T cells. After a median follow-up time of 27.0 (range, 24.0–29.3) months, the overall response rate and complete response rate were 95% (19/20 patients) and 85% (17/20 patients), respectively, and 35% (7/20) of patients proceeded to SCT. Six patients experienced disease relapse with a median time-to-relapse of 6 (range, 4.0–10.9) months, and 4 of these 6 patients were found to have lost CD7 expression on tumor cells. Progression-free survival (PFS) and overall survival (OS) rates 24 months after treatment were respectively 36.8% (95% CI, 13.8–59.8%) and 42.3% (95% CI, 18.8–65.8%), with median PFS and OS of respectively 11.0 (95% CI, 6.7–12.5) months and 18.3 (95% CI, 12.5–20.8) months. Previously reported short-term adverse events ( 30 days after treatment included five infections and one grade 4 intestinal GVHD. Despite good CD7 CAR T-cell persistence, non-CAR T and natural killer cells were predominantly CD7-negative and eventually returned to normal levels in about half of the participants. Conclusions In this 2-year follow-up analysis, donor-derived CD7 CAR T-cell treatment demonstrated durable efficacy in a subset of patients with r/r T-ALL. Disease relapse was the main cause of treatment failure, and severe infection was a noteworthy late-onset adverse event. Trial registration ChiCTR2000034762.

This study proposed a new method for homogenizing continuous casting blooms based on solidification simulation calculations and industrial tests. The text describes a theoretical analysis of the solidification route of a cast billet of high-carbon alloy steel (B300A) under different process conditions. It summarizes the changing law of different under-pressure process parameters and under-pressure efficiency. The text also presents a solution to the seriousness of center shrinkage defects in the continuous casting of a large square billet of high-carbon alloy steel with the synergistic control technology of mixed light and heavy mixing under pressure. The study indicates that the center carbon segregation index of a high carbon steel continuous casting billet is 1.05, with a carbon extreme difference of not more than 0.08% and a proportion of 98.4%. Additionally, the center shrinkage is not more than a 0.5 level with a proportion of 99.5%. Meanwhile, the internal quality of cast billets has been improved, allowing for the rolling of large-size bars with a low consolidation ratio. The pass rate for internal ultrasonic flaw detection using the GB/T4162A grade is now higher than 99.95%, significantly reducing process costs and improving production efficiency for continuous casting and rolling.

Identifying the spatial heterogeneity characteristics of ecosystem services (ESs) and their influencing mechanisms is crucial for regional ecological management and sustainable development. This study quantified net primary production (NPP), water yield (WY), soil conservation (SC), sand fixation (SF), habitat quality (HQ) and total ecosystem service (TES) on the Qinghai Tibet Plateau (QTP), and then analyzed the spatial–temporal characteristics of ESs. The geographical detector model (GDM), geographically weighted regression (GWR) model, and constraint lines were used to explore the effects of influencing factors on ESs. The results showed that (1) NPP, WY, SC, HQ and TES showed an increasing trend from 2000 to 2018, while SF showed a decreasing trend. NPP, WY, SC and TES showed a spatial distribution pattern of “high values in the southeast and low values in the northwest”, while the high values of SF were located in the northern QTP and the high values of HQ were located in the southern QTP. (2) NDVI was the key factor influencing NPP, SC, HQ and TES, and precipitation dominated WY, and SF was mainly influenced by Largest patch index (LPI). (3) In most of the study areas, the natural factors (precipitation, temperature, DEM, slope and NDVI) had positive effects on NPP, SC and TES and both positive and negative effects on WY, SF and HQ. The human factors (Resident, Road and LPI) had positive effects on HQ and both positive and negative effects on other ESs. There were mainly non-monotonic constraint effects of influencing factors on ESs. Overall, natural factors had a much stronger influence on ESs than human factors, and the interactions among factors were much stronger than their independent effects. The results of this study can provide a basis for ecological protection on the QTP.

Abstract Background Mucosal-associated invariant T (MAIT) cells are innate-like T cells, some studies have reported that the number of circulating MAIT cells reduced in patients with acute graft-versus-host-disease (aGVHD) development. However, the role of donor MAIT cells on aGVHD development and subsequent functional change still remain unclear. Methods The study recruited 86 patients with hematological malignancies who underwent allogeneic hematopoietic cell transplantation (HCT) from May 1, 2018 to June 30, 2019. MAIT cells, their subset, and cytokine levels were measured by flow cytometry. Gray’s test was used to assess the impact of graft MAIT cell proportion and number on aGVHD incidence. The Cox proportional hazard model was used in the multivariate analysis. The comparison for continuous variables was assessed using Mann–Whitney analysis. RNA-sequencing was performed to investigate the possible molecular pathway changes. Results Our study showed that the proportion of MAIT cells in grafts was not different from normal controls, but the CD4/8 subsets were altered. Taking the median of the proportion and number of MAIT cells in the graft as the threshold, the results showed that the incidence of grade B-D aGVHD in patients with MAIT cell proportion ≥ 3.03% was significantly higher than that in patients with MAIT cell proportion

Study region: The source regions of the Yellow River and Yangtze River in the central-eastern part of the Tibetan Plateau. Study focus: Hydrological model is an important tool in the simulation of watershed hydrology. However, as more and more basic geographic data becomes publicly available and shared globally, researchers are developing a 'symptom' of difficulty in choosing data, so a systematic comparative analysis for model input data selection is necessary. We tested the effects of different types, sources, and resolutions of input data on the model output results based on the SWAT model, and focused on the mechanism of the role of different input data in the model and how to select an appropriate input data for similar studies. New hydrological insights for the region: The results show that: the meteorological data is crucial in the model's runoff simulation, and ground meteorological observation station data outperforms reanalysis data such as CFSR. Optimizing CFSR significantly improves the model's performance. The DEM resolution minimally impacts runoff simulation, as the difference in results is primarily due to the topographic characteristics of the watershed. DEM selection should consider TWI complexity and its compatibility with the watershed network. The selection of LULC data has little effect on the simulation, and the best input data combination is OBS + 90 m DEM + CNLULC. These findings assist input data selection for similar watersheds using the SWAT model.

Study region: The Natural Forest Protection Project area in China (NFPP). Study focus: Applying the Pattern-Process-Mechanism theory, we analyzed the spatiotemporal differentiation pattern and change trend of evapotranspiration (ET) in NFPP from 2000 to 2018. We employed methods such as geographical detectors and path analysis to identify the dominant factors and response mechanisms of ET changes in NFPP, specifically those resulting from vegetation recovery and climate change. New hydrological insights for the region: Our study reveals that ET in NFPP exhibits a fluctuating increasing trend, rising from 299.155 mm/yr in 2000–336.805 mm/yr in 2018, with an annual average of 316.486 mm/yr and a change rate of 2.092 mm/yr. The proportion of areas with decreasing ET trend in NFPP (50.944%) is approximately equal to that of areas with increasing ET trend (49.056%). Vegetation restoration (18.959%), water conditions (18.460%), and thermal conditions (56.526%) are the predominant factors that influence the changes and distribution of ET in NFPP. However, the trend of ET and its dominant factors vary by region. We emphasize that, given the circumstances of climate change and the continued increase in ET, future vegetation recovery strategies must consider water usage, as this has critical practical implications for the sustained maintenance of regional ecological engineering outcomes.

Reanalysis datasets provide a reliable reanalysis of climate input data for hydrological models in regions characterized by limited weather station coverage. In this paper, the accuracy of precipitation, the maximum and minimum temperatures of four reanalysis datasets, the China Meteorological Assimilation Driving Datasets for the SWAT model (CMADS), time-expanded climate forecast system reanalysis (CFSR+), the European Centre for Medium-Range Weather Forecast Reanalysis (ERA). and the China Meteorological Forcing Dataset (CMFD), were evaluated by using data from 28 ground-based observations (OBs) in the Source of the Yangtze and Yellow Rivers (SYYR) region and were used as input data for the SWAT model for runoff simulation and performance evaluation, respectively. And, finally, the CMADS was optimized using Integrated Calibrated Multi-Satellite Retrievals for Global Precipitation Measurement (AIMERG) data. The results show that CMFD is the most representative reanalysis data for precipitation characteristics in the SYYR region among the four reanalysis datasets evaluated in this paper, followed by ERA5 and CFSR, while CMADS performs satisfactorily for temperature simulations in this region, but underestimates precipitation. And we contend that the accuracy of runoff simulations is notably contingent upon the precision of daily precipitation within the reanalysis dataset. The runoff simulations in this region do not effectively capture the extreme runoff characteristics of the Yellow River and Yangtze River sources. The refinement of CMADS through the integration of AIMERG satellite precipitation data emerges as a potent strategy for enhancing the precision of runoff simulations. This research can provide a reference for selecting meteorological data products and optimization methods for hydrological process simulation in areas with few meteorological stations.

Abstract Natural killer (NK) cells are unique innate immune cells and manifest rapid and potent cytotoxicity for cancer immunotherapy and pathogen removal without the requirement of prior sensitization or recognition of peptide antigens. Distinguish from the T lymphocyte-based cythotherapy with toxic side effects, chimeric antigen receptor-transduced NK (CAR-NK) cells are adequate to simultaneously improve efficacy and control adverse effects including acute cytokine release syndrome (CRS), neurotoxicity and graft-versus-host disease (GVHD). Moreover, considering the inherent properties of NK cells, the CAR-NK cells are “off-the-shelf” product satisfying the clinical demand for large-scale manufacture for cancer immunotherapy attribute to the cytotoxic effect via both NK cell receptor-dependent and CAR-dependent signaling cascades. In this review, we mainly focus on the latest updates of CAR-NK cell-based tactics, together with the opportunities and challenges for cancer immunotherapies, which represent the paradigm for boosting the immune system to enhance antitumor responses and ultimately eliminate malignancies. Collectively, we summarize and highlight the auspicious improvement in CAR-NK cells and will benefit the large-scale preclinical and clinical investigations in adoptive immunotherapy.

Abstract Objectives Ruxolitinib, a Janus kinase (JAK) 1/2 inhibitor, demonstrates efficacy for treating steroid‐resistant acute graft‐versus‐host disease (SR‐aGVHD) following allogeneic stem cell transplantation (allo‐HSCT). Myeloid‐derived suppressor cells (MDSCs) have a protective effect on aGVHD via suppressing T cell function. However, the precise features and mechanism of JAK inhibitor‐mediated immune modulation on MDSCs subsets remain poorly understood. Methods A total of 74 SR‐aGVHD patients treated with allo‐HSCT and ruxolitinib were enrolled in the present study. The alterations of MDSC and regulatory T cell (Treg) populations were monitored during ruxolitinib treatment in responders and nonresponders. A mouse model of aGVHD was used to evaluate the immunosuppressive activity of MDSCs and related signalling pathways in response to ruxolitinib administration in vivo and in vitro. Results Patients with SR‐aGVHD who received ruxolitinib treatment achieved satisfactory outcomes. Elevation proportions of MDSCs before treatment, especially polymorphonuclear‐MDSCs (PMN‐MDSCs) were better to reflect the response to ruxolitinib than those in Tregs. In the mouse model of aGVHD, the administration of ruxolitinib resulted in the expansion and functional enhancement of PMN‐MDSCs and the effects could be partially reversed by an anti‐Gr‐1 antibody in vivo. Ruxolitinib treatment significantly elevated the suppressive function of PMN‐MDSCs through reactive oxygen species (ROS) production by Nox2 upregulation as well as bypassing the activated MAPK/NF‐κB signalling pathway. Additionally, ex vivo experiments demonstrated that ruxolitinib prevented the differentiation of mature myeloid cells and promoted the accumulation of MDSCs by inhibiting STAT5. Conclusions Ruxolitinib enhances PMN‐MDSCs functions through JAK/STAT and ROS‐MAPK/NF‐κB signalling pathways. Monitoring frequencies and functions of MDSCs can help evaluate treatment responses to ruxolitinib.

Dryland ecosystems are crucial components within the Earth and atmospheric systems, and a number of projects have been implemented to restore vegetation in drylands in the recent years. However, these regions are experiencing extreme climatic changes, and the impact of these projects are unclear. To gain a better understanding of the effectiveness of these projects, we employed water use efficiency (WUE), the ratio of gross primary production (GPP) to terrestrial evapotranspiration (ET), to predict future dryland ecosystem dynamics, and detected the underlying causes of WUE changes. We analysed the WUE trends and the decomposed components of WUE, namely the GPP to plant transportation ratio (GPP/T) and the plant transportation to terrestrial evapotranspiration (T/ET) ratio, between 2003 and 2018 in China’s drylands. The results indicated that 70.3 % of the study area had an upward WUE trend, and T/ET was the dominant factor of the WUE trend in 74.9 % of the areas investigated. Furthermore, the increase in T/ET was associated with growth in leaf area index (LAI). The rising WUE rate in the vegetation restoration project areas was significantly higher than that in other areas along with the drought gradient (P

Abstract CD19 chimeric antigen receptor (CAR)-T cells have been used to treat patients with refractory chronic lymphocytic leukemia (CLL). However, approximately 50% of patients do not respond to this therapy. To improve the clinical outcome of these patients, it is necessary to develop strategies with other optimal targets to enable secondary or combinational CAR-T cell therapy. By screening a panel of surface antigens, we found that CD32b (FcγRIIb) was homogeneously expressed at high site density on tumor cells from CLL patients. We then developed a second-generation CAR construct targeting CD32b, and T cells transduced with the CD32 CAR efficiently eliminated the CD32b+ Raji leukemic cell line in vitro and in a mouse xenograft model. Furthermore, CD32b CAR-T cells showed cytotoxicity against primary human CLL cells that were cultured in vitro or transplanted into immunodeficient mice. The efficacy of CD32b CAR T cells correlated with the CD32b density on CLL cells. CD32b is not significantly expressed by non-B hematopoietic cells. Our study thus identifies CD32b as a potential target of CAR-T cell therapy for CLL, although further modification of the CAR construct with a safety mechanism may be required to minimize off-target toxicity.

Human Treg cells are central to immune tolerance, yet their heterogeneity and differentiation remain incompletely understood. Here the authors perform single-cell RNA and T cell receptor sequencing to resolve Treg cells from healthy individuals and patients with or without acute graft-versus-host disease revealing Treg complexity in health and disease.

Ecological risk assessment (ERA) is an important element in territorial spatial planning, and the integration of ecosystem services into ERA is a current hot topic. In this study, taking the Tibetan Plateau as an example, a two-dimensional matrix is constructed based on the probability of risk occurrence and the possible loss caused by the risk for ecological risk assessment, with topographic sensitivity, ecological resilience, landscape vulnerability, and ecological sensitivity as probability representations, and the degradation of key ecosystem services as loss representations to improve the traditional ERA model. The results show that: (1) the probability shows a distribution pattern of high in the northwest and low in the southeast, with high-probability dominating, accounting for about 55.94%; the loss shows a distribution of low in the north and high in the south, with 30.54% of the region experiencing increased losses. (2) The ecological risk level is dominated by middle-high and high, the sum of the two accounts for 55.44%, while low and middle-low risk areas account for only 10.47%. Based on the proportion of high-risk in 45 cities, the risk control priority areas are identified: Naqu, Ali and Rikaze. (3) The Moran's I for probability, loss, and risk were 0.878, 0.615, and 0.567, respectively, indicating that all three showed significant positive spatial autocorrelation. The negative correlation between probability and loss indicates that there is spatial heterogeneity between them.

Although enols and enolates addition to unactivated alkynes is used for carbon-carbon bond modification a catalytic asymmetric alkyne with 1,3-dicarbonyl compound has been elusive. Here, the authors achieve this using the synergistic activation of chiral N,N′-dioxide-indium(III) or nickel(II) Lewis acid and achiral gold(I) π-acid.”

Water resources are a basic need for social sustainable development and human existence. As an important national strategy for water resources security, spatial and temporal patterns and driving mechanisms of water yield ecosystem services on the Qingzang Plateau (QP) are critical for water resources management, optimal water allocation and the improvement of ecological water protection efficiency. However, only a few relevant studies are currently available. In this study, we simulated the water yield (WY) of the QP over 34 years, from 1982 to 2015, using the InVEST model and analyzed the spatiotemporal dynamic relationships between WY and climate change as well as between WY and vegetation change, using geographically weighted regression (GWR) models. The results showed that: 1) from 1982 to 2015, the WY of the QP increased at an average rate of 3.8 mm/yr; 2) WY presented a reduced spatial pattern from southeast to northwest; and 3) the WY driving factors have individual and spatial differences. In terms of the area percentage in promoting WY when analyzing each driving factor, precipitation (99.8%) and air pressure (53.3%) played the major roles in promoting WY, while temperature (71.9%), wind speed (57.2%), net primary productivity (87.2%), radiation (68.3%) and lake (87.7%) played negative roles. The areas where WY are dominated by temperature are the largest (41.1%), and followed by areas dominated by pressure (19.7%) and precipitation (18.5%). The results of this study provide scientific support for formulating regional water resources policy, social and economic development planning and other macro decisions for the QP.

The investigation of reactivity of β,γ-unsaturated carbonyl compounds is of great synthetic value, especially in asymmetric transformations. Here, the authors report a catalytic asymmetric tandem isomerization/α-Michael addition of β,γ-unsaturated 2-acyl imidazoles in presence of chiral N,N′-dioxide metal catalysts.

There is an extremely complex interactive coupling relationship between social development and ecological protection, and how to achieve the coordinated development of the two is a hot topic in sustainable development research. This paper constructs a comprehensive evaluation index system of Social-Ecological System(SES) on the Qinghai-Tibet Plateau, and then multi-scale analysis of the coupling coordination status and temporal-spatial evolution characteristics of the SES from 2003 to 2018 based on the coupling coordination degree model(CCDM), scissors difference, etc.The results show that: (1) the comprehensive evaluation index of social system shows an increasing trend, with a growth rate of 16.46% and a spatial distribution pattern of high in the northeast and low in the southwest; the ecosystem comprehensive evaluation index fluctuates down, with a decline rate of 32.80% and a spatial distribution pattern of low in the northwest and high in the southeast. (2) There are differences in the types of coordination among different scales: the Qinghai-Tibet Plateau as a whole is mild disorders; on the provincial scale, the lowest coupling coordination degree is mild disorders in Tibet, followed by primary coordination in Sichuan and Qinghai, and Gansu, Yunnan, and Xinjiang are the highest, all of which are moderate coordination; on the county scale, the proportion of severe disorders, moderate disorders and primary coordination decreased by 7.03%, 2.63% and 7.20% respectively, while mild disorders and moderate coordination increased by 11.28% and 5.59% respectively. (3) The scissors difference rising in volatility, and the difference between the evolutionary trends of the two subsystems has increased. (4) The dynamic coupling degree is distributed in the interval of [45°, 90°], indicating that the SES of the Qinghai-Tibet Plateau is in a stage of common development, but the conflicts between the two are beginning to emerge. An in-depth analysis of the internal interaction process and the coupling coordination status of the SES can provide a theoretical basis and policy reference for sustainable development of the Qinghai-Tibet Plateau.

The present study investigates the impact of rare earth Ce addition and holding time on inclusions in offshore engineering steel containing arsenic. The analysis was conducted using scanning electron microscopy and energy spectrum (SEM-EDS), automatic statistics of inclusions, and thermodynamic analysis. The results indicate that prior to the addition of rare earth Ce, the typical inclusions in the steel were Al _2 O _3 , MnS and Al _2 O _3 -MnS, with an average size of approximately 2.51 μ m and an irregular or stripe morphology. Upon the addition of rare earth Ce, the inclusions in the steel were transformed into Ce-S(-O), Ce-As(-O) and Ce-S-As(-O), with a smaller average size and a spherical or ellipsoidal morphology. The smallest average size of inclusions in the steel was observed when rare earth Ce was added and held for 5 min; this size was 33% smaller than that prior to the addition of Ce. The thermodynamic calculation revealed that the Ce-S(-O) inclusion is formed in molten steel, while the Ce-As(-O) inclusion is formed during the solidification stage. As element replaced parts of the S and O elements in the Ce-S(-O) inclusion and formed the Ce-S-As(-O) complex inclusion, characterized by a double-layered structure.

Aging is a multifaceted process regulated by multiple cellular pathways, including the proteostasis network. Pharmacological or genetic enhancement of the intracellular proteostasis network extends lifespan and prevents age-related diseases. However, how proteostasis is regulated in different tissues throughout the aging process remains unclear. Here, we show that Drosophila homologs of Cubilin- and Amnionless (dCubilin and dAMN, respectively)-mediated protein reabsorption (CAMPR) from hemolymph insect blood by nephrocytes modulate longevity through regulating proteostasis in muscle and brain tissues. We find that overexpression of dAMN receptor in nephrocytes extends lifespan, whereas nephrocyte-specific dCubilin or dAMN RNAi knockdown shortens lifespan. We also show that CAMPR in nephrocytes regulates proteostasis in hemolymph and improves healthspan. In addition, we show that enhanced CAMPR in nephrocytes slows down the aging process in muscle and brain by maintaining the proteostasis network in these tissues. Altogether, our work has revealed an inter-organ communication network across nephrocytes and muscle/neuronal tissue that is essential for maintaining proteostasis, and to delay senescence in these organs. These findings provide insight into the role of renal protein reabsorption in the aging process via this tele-proteostasis network.

Desert plants are the main component of species diversity in desert ecosystems, and studying the anatomy and function of desert plant xylem is of great significance for understanding climate sensitivity and adaptation mechanisms to arid ecosystems. In this study, 11 sampling points were selected in the region starting from the Loess Plateau to the Jungar Basin, the taproot anatomy materials of 9 samples of Zygophyllaceae plants were collected, and the water conduction strategies and spatial distribution characteristics of these species were analyzed. The age, growth rate, vessel number, vessel fraction, vessel area within a fixed measurement range (TVA), MVA, water conductivity (TKp, MKp) and vessel diameter ranged between 1 and 27 years, 43.67 and 678.10 μm/year, 20 and 1952, 4.43 and 26.58%, 8009.62 and 192069.12 μm², 27.63 and 2164.33 μm², 0.417 and 364.97 kg m−1 MPa−1 s−1, 0.000624 and 7.60 kg m−1 Mpa−1 s−1, and 5.57 and 73.87 μm, respectively. The number of root vessels (R = 0.27, p > 0.05) of Zygophyllaceae plants decreased with the decrease in precipitation, and the average vessel area (R = −0.28, p > 0.05) and hydraulic diameter (R = −0.29, p > 0.05) showed an upward trend. This shows that the water hydraulic efficiency priority strategy may be adopted in the root system of Zygophyllaceae plants in severe drought stress condition, and the water hydraulic safety priority strategy may be used in mild drought stress conditions. With the increase in temperature, the root age of Zygophyllaceae plants showed an increasing trend, and the growth rate showed a downward trend, indicating that the radial growth of the roots of Zygophyllaceae plants is mainly affected by temperature. Altitude influences plant growth by affecting temperature and precipitation in arid habitats. The findings of the present study on root xylem anatomical characteristics and life history strategies provides a scientific basis for the ecological restoration of vegetation in arid and semi-arid areas of China.

In this paper, biomimetic spines on puffer skin were taken as the study object, and numerical simulation was used to study the effect of the spinal arrangement on drag reduction characteristics. The results showed that the drag reduction decreased with the increasing arrangement position (AP) and inflow angle (β), and the drag reduction of the average arrangement was obviously better than that of the staggered arrangement. Moreover, drag reduction increased gradually as the arrangement quantity-R/T (AQR/AQT) increased. The drag reduction increased first and then decreased with the increasing arrangement spacing-R (ASR), and the drag reduction increased as the arrangement spacing-T (AST) increased. In short, the greater the spinal quantity and spacing, the better the drag reduction effect; however, it was affected by the arrangement position, which made the drag reduction effect reduce. More notably, the optimal drag reduction reached 23.1% when the arrangement parameters were the average arrangement, β = 0°, AP = 0 mm, AQR = 11, AQT = 5, ASR = 6.5 mm, and AST = 3.5 mm. Overall, the spinal arrangement parameters can be effectively regulated until the optimal drag reduction was realized, and this work provides a valuable reference for future studies on drag reduction for engineering applications.

Chimeric antigen receptor (CAR)-T cell therapy emerges as a new treatment for refractory or relapsed (r/r) B-cell non-Hodgkin lymphoma (B-NHL); however, the overall response rate (ORR) of which in the B-NHL patients is much lower compared to the patients with r/r B acute lymphoblastic leukemia (B-ALL). We previously confirmed that sequential infusions of CD20 and CD22 CAR-T cells significantly improved the prognosis of the B-NHL patients, while some advanced patients still progressed to death during these CAR-T cell treatments. In this study, we showed that timely sequential administration of the second CAR-T cells could enhance expansion of prior CAR-T cells with stronger tumor-killing capacity in vitro and in vivo. We further conducted compassionate treatments on two advanced B-NHL patients with short-interval sequential infusions of CD19/22/20 CAR-T cells. Disease progression was observed in both patients after primary CAR-T cell infusion but robust re-expansion of prior CAR-T cells and anti-tumor effects was induced by infusion of a secondary CAR-T cells. These results indicate sequential infusions of CAR-T cells with a short interval may improve therapeutic efficacy in the B-NHL patients by promoting expansion of prior CAR-T cells.

Asymmetric isocyanide-based multicomponent reactions are elegant, yet challenging, strategies to access valuable N-heterocycles. Here, the authors employ a chiral Mg(II) -N,N′-dioxide catalyst in three- or four-component reactions to obtain chiral tetrazoles and devise a dearomative [3+2] annulation reaction of isoquinolines.

Regulatory T (Treg) cells are important for maintaining immune homeostasis by suppressing immune cell activation, but how the Treg cell pool is maintained is still unclear. Here the authors show that a kinase, Lkb1, operates in dendritic cells (DC) to inhibit Treg cell expansion and immunosuppression via mechanisms involving NF-kB/OX40L signalling.

Myeloid cells have been identified as hematopoietic stem cell (HSC)-regulating cells. However, the mechanisms by which myeloid cells regulate the function of HSCs are not fully defined. Our previous study indicated that the HSCs are over-expanded in Vav1-Cre;Rheb1fl/fl mice. Here, using in vivo and in vitro models, we found that Rheb1-deficient neutrophils remodeled the bone marrow environment and induced expansion of HSCs in vivo. Further studies showed that loss of Rheb1 impaired neutrophils’ ability to secrete IL-6, led mesenchymal stem cells (MSCs) to produce more SCF, and promote HSC proliferation. We further found that IL-6 suppressed SCF mRNA expression in human MSCs. Interesting, the high level of IL-6 was also related with poor survival of chronic myeloid leukemia (CML) patients, and higher expression of IL-6 in CML cells is associated with the lower expression of SCF in MSCs in patients. Our studies suggested that blocking IL-6 signaling pathway might stimulate MSCs to secrete more SCF, and to support hematopoietic stem/progenitor cells proliferation.

Alveolar macrophages (AMs) are pivotal for maintaining lung immune homeostasis. We demonstrated that deletion of liver kinase b1 (Lkb1) in CD11c+ cells led to greatly reduced AM abundance in the lung due to the impaired self-renewal of AMs but not the impeded pre-AM differentiation. Mice with Lkb1-deficient AMs exhibited deteriorated diseases during airway Staphylococcus aureus (S. aureus) infection and allergic inflammation, with excessive accumulation of neutrophils and more severe lung pathology. Drug-mediated AM depletion experiments in wild type mice indicated a cause for AM reduction in aggravated diseases in Lkb1 conditional knockout mice. Transcriptomic sequencing also revealed that Lkb1 inhibited proinflammatory pathways, including IL-17 signaling and neutrophil migration, which might also contribute to the protective function of Lkb1 in AMs. We thus identified Lkb1 as a pivotal regulator that maintains the self-renewal and immune function of AMs.

Ag-doped ZnO nanorods growth on a PET-graphene substrate (Ag-ZnO/PET-GR) with different Ag-doped content were synthesized by low-temperature ion-sputtering-assisted hydrothermal synthesis method. The phase composition, morphologies of ZnO, and electrical properties were analyzed. Ag-doping affects the initially perpendicular growth of ZnO nanorods, resulting in oblique growth of ZnO nanorods becoming more obvious as the Ag-doped content increases, and the diameter of the nanorods decreasing gradually. The width of the forbidden band gap of the ZnO films decreases with increasing Ag-doped content. For the Ag-ZnO/PET-GR composite structure, the Ag-ZnO thin film with 5% Ag-doped content has the largest carrier concentration (8.1 × 1018 cm−3), the highest mobility (67 cm2 · V−1 · s−1), a small resistivity (0.09 Ω·cm), and impressive electrical properties.

The spatiotemporal regimes of glacier runoff (GR) under a warming climate are of great concern, especially in dryland areas in northwestern China (DAC). Due to the difficulty of observing GR, little attention has been given to the spatiotemporal change in GR at regional scales. This study uses the regional individual glacier mass balance (GMB) dataset developed by digital elevation models (DEMs) to simulate the spatiotemporal regime of GR using atmospheric parameters considering both ablation and accumulation processes on glaciers. In this study, GR, including glacier meltwater runoff (MR) and delayed water runoff (DR) of the DAC, was quantitatively assessed at a catchment scale from 1961 to 2015. The total annual GR in the DAC was (100.81 ± 68.71) × 108 m3 in 1961–2015, where MR accounted for 68%. Most basins had continuously increasing tendencies of different magnitudes from 1961 to 2015. The least absolute shrinkage and selection operator (LASSO) and random forest techniques were used to explore the contributions of climate factors and glacier physical properties to GR, and the results indicated that climate factors could explain 56.64% of the variation. In comparison, the remaining 43.36% could be explained by the physical properties of glaciers themselves (i.e., degree-day factor on ice, degree-day factor on snow, glacier median height, aspect, and slope). This study not only improves our understanding of the spatiotemporal change in GR in the drylands of northwestern China at spatial and temporal resolutions but also highlights the role of physical properties in explaining the heterogeneous dynamics among GRs unlike previous studies that only emphasize rising temperatures.

Drought is the most widespread climatic extreme that has negative impacts on ecohydrology. Studies have shown that drought can cause certain degrees of disturbances to different ecohydrological variables, but the duration and severity thresholds of drought that are sufficient to cause changes in ecohydrological variables remain largely unknown. At the same time, we should not ignore the dynamic variation of drought’s effect on ecohydrological variables under the condition of climate change. Here, we derived the thresholds of several ecohydrological variables in response to drought in a historical period (1982–2015), including evapotranspiration (ET), soil moisture (SM), the vapor pressure deficit (VPD) and the normalized difference vegetation index (NDVI), and we projected the occurrence probability’s change trend of drought events that cause changes in ecohydrological variables under future climate change. The results show that the impact of drought on ecohydrological variables is not dependent on drought indicators. ET and NDVI were expected to decrease in most parts of the world due to increases in radiation (RAD) and temperature (TEMP) and decreases in precipitation (PRE) during drought periods. SM decreased in most regions of the world (93.47%) during the drought period, while VPD increased in 85.41% of the globe. The response thresholds for different ecohydrological variables to drought in the same area did not differ significantly (especially for ET, SM and VPD). When a drought lasted for 8 to 15 months and the corresponding drought severity reached 10 to 15 (the inverse of the cumulative values of the drought index when the drought occurs), the drought caused changes in the ecohydrological variables in most regions of the world. Compared with arid and semiarid regions, ecohydrological variables are more sensitive to drought in humid and semihumid regions (p < 0.05), and high-intensity human activities in different climatic conditions increased significantly the severity of drought processes. Between 2071 and 2100, more than half of the world’s ecohydrological variables are expected to be more susceptible to drought disturbances (regions with shorter return periods of drought events that cause significant changes in ET, SM, VPD and NDVI account for 60.1%, 64.4%, 59.6% and 54.5% of the global land area, respectively).

Dephosphorization has always been a challenging task in the steelmaking process. In the traditional studies, CaO-based steelmaking slag was usually treated as a homogeneous liquid phase to analyze the dephosphorization equilibrium between molten slag and hot metal (slag/metal). In fact, the slag shows a coexisting state of both liquid and solid at steelmaking temperature. In recent years, multi-phase slag refining has been proposed and proved to be an efficient technique to improve the utilization of solid lime and the dephosphorization efficiency in steelmaking practices. The present review focuses on hot issues for the multi-phase slag refining, including the dissolution of solid lime to the molten slag, the formation of 2CaO·SiO2 and phosphorus-rich solid solution of 2CaO·SiO2-3CaO·P2O5, as well as the phosphorus distribution ratio between the solid and liquid phases in the multi-phase slag. In addition to the discussion on thermal and kinetic factors of dephosphorization, the necessity of further research is proposed on the dissolution behavior of phosphorus-rich solid solution and phosphorus redistribution in the slag of high temperature, high basicity and high FeO in the final stage of converter steelmaking. Then, a more applicable dynamic model of dephosphorization could be expected considering the equilibrium not only between molten slag and liquid metal but also among the phases of the multi-phase slag in steelmaking process. Furthermore, the modelling work is beneficial for the fine control of the BOF process and the development of intelligent manufacturing in steelmaking industry.

The transport of eroded soil to rivers changes the nutrient cycles of river ecosystems and has significant impacts on the regional eco-environment and human health. The Loess Plateau, a leading vegetation restoration region in China and the world, has experienced severe soil erosion and nutrient loss, however, the extent to which vegetation restoration prevents soil erosion export (to rivers) and it caused nutrient loss is unknown. To evaluate the effects of the first stage of the Grain for Green Project (GFGP) on the Loess Plateau (started in 1999 and ended in 2013), we analyzed the vegetation change trends and quantified the effects of GFGP on soil erosion export (to rivers) and it caused nutrient loss by considering soil erosion processes. The results were as follows: (1) in the first half of study period (from 1982 to 1998), the vegetation cover changed little, but after the implementation of the first stage of the GFGP (from 1999 to 2013), the vegetation cover of 75.0% of the study area showed a significant increase; (2) The proportion of eroded areas decreased from 41.8 to 26.7% as a result of the GFGP, and the erosion intensity lessened in most regions; the implementation significantly reduce the soil nutrient loss; (3) at the county level, soil erosion export could be avoided significantly by the increasing of vegetation greenness in the study area (R = −0.49). These results illustrate the relationships among changes in vegetation cover, soil erosion and nutrient export, which could provide a reference for local government for making ecology-relative policies.

Drylands account for 41% of the world’s terrestrial surface, affect more than two billion people, and play a crucial role in global circulation and even global climate change. Therefore, the investigation of agricultural expansion and land degradation regions is a critical part of understanding the human-land coupling systems in dryland ecosystems. The specific distributions of agricultural expansion or land degradation in arid areas were obtained by using three indicators of global Vegetation Continuous Fields (VCF) data, in which expanding agriculture is usually characterized by tree canopy (TC) loss and short vegetation (SV) gain, while land degradation is characterized by a decrease in short vegetation and an increase in bare ground (BG). Assessing the partial correlation of natural or socioeconomic factors and indicators of agricultural expansion or land degradation while the structural equation model was established, we found that (1) the vegetation situation in the global arid region is relatively stable, and the normalized difference vegetation index (NDVI) value in the Asian arid region is increasing annually; (2) precipitation in the global arid region has a highly positive correlation with TC and a highly negative correlation with BG, while temperature is strongly correlated with SV except in arid areas; (3) both agricultural expansion and land degradation regions are dominated by natural factors, especially temperature; and (4) the control of socioeconomic factors is not closely related to the original land use/cover types in dryland regions.

EH36 offshore engineering steels with varied yttrium-based rare earth content were prepared by trials in industrial production. The effects of yttrium-based rare earth on the inclusions and cryogenic temperature impact properties of EH36 offshore engineering steel were investigated by scanning electron microscopy, automatic statistics of inclusions, thermodynamic analysis and fracture morphology analysis. Yttrium-based rare earth could refine the inclusions and modify irregular Al2O3 and MnS inclusions into small, spherical, regular rare earth inclusions. The optimal impact properties were found in EH36 steel with 0.020 wt.% yttrium-based rare earth. Compared with 0RE steel, the RE-inclusions were within 3 μm (91.95% of total inclusions) in diameter and were spherical or quasi-spherical when dispersed in 200RE steel. Meanwhile, the cryogenic temperature impact properties significantly increased: 200RE steel impact properties were increased by 245.1% at −80 °C.

Bottom blowing agitation plays a crucial role in improving the reaction kinetics condition of molten bath during the steelmaking process. Herein, the influence of bottom blowing mode on the flow and mixing characteristics of molten bath and the abrasion characteristics of refractory lining in a 6:1 scaled-down model of a 100 t converter were investigated using physical and numerical simulations together. Eight bottom blowing modes were designed (uniform, three-point linear co-direction, three-point linear unco-direction, two-point linear, circumferential linear, A-type, V-type, and triangle alternating). The results indicated that bottom blowing mode has a significant effect on the local flow field at the inner ring of bottom tuyeres, the velocity interval distribution, and the turbulent kinetic energy, which in turn determines the tracer diffusion path and rate as well the mixing time of molten bath. Reasonable non-uniform bottom blowing modes promote the interaction between the various stirring sub-zones of the molten bath. Among them, the three-point linear co-direction mode and A-type mode have the highest mixing efficiency under the conditions of bottom blowing and combined blowing, respectively, which is superior to the uniform mode. In addition, the bottom blowing mode changed the location and degree of abrasion of the refractory lining, and the total abrasion of the non-uniform mode was reduced. The average value and fluctuation degree of integral wall shear stress for the A-type mode were minimal.