1. Preliminary research on LncRNA ATP2B2-IT2 in neovascularization of diabetic retinopathy
- Author
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Yuan Yuan, Anming Zhu, Lan Zeng, Xiaocong Wang, Ying Zhang, Xiaofeng Long, Jie Wu, Meng Ye, Junhao He, and Wei Tan
- Subjects
Diabetic retinopathy ,LncRNA ATP2B2-IT2 ,Proliferation ,Migration ,Neovascularization ,Ophthalmology ,RE1-994 - Abstract
Abstract Objective Diabetic retinopathy (DR) is a common complication of diabetes, and recent findings have shown that long noncoding RNAs (lncRNAs) may be involved in its pathogenesis. Through bioinformatics analysis, we found that lncRNA ATP2B2-IT2 may be involved in this process. This study primarily investigated the expression of the lncRNA ATP2B2-IT2 in human retinal microvascular endothelial cells (HRMECs) under high-glucose conditions and its effects on HRMEC proliferation, migration, and neovascularization. Methods We used RT‒PCR to assess the expression levels of lncRNA ATP2B2-IT2 and vascular endothelial growth factor (VEGF) in HRMECs under normal glucose (5.5 mmol/L) and high glucose (30 mmol/L) conditions. HRMECs were subsequently divided into four groups: the normal glucose (NG), high glucose (HG), high glucose with lncRNA ATP2B2-IT2 silencing (HG + si-lncRNA ATP2B2-IT2), and high glucose with silencing control (HG + si-NC) groups. The expression levels of the lncRNA ATP2B2-IT2 and VEGF in each group were determined using RT‒PCR. Thereafter, cell proliferation, migration, and neovascularization were assessed using CCK-8, Transwell, and tube formation assays, respectively. Results RT‒PCR revealed that the expression levels of the lncRNA ATP2B2-IT2 and VEGF were greater in the HG group than in the NG group (P
- Published
- 2024
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