19 results on '"Xiaofen Xiao"'
Search Results
2. A dual-mode sensor based on colorimetric and Tyndall effect of gold nanoparticles for ultra-sensitive detection of Hg2+
- Author
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Dandan Li, Qilin Wen, Yanyu Zhou, Dan Li, Huai Xi, Guidan Huang, Jinhua Liang, Xiaofen Xiao, and Wenyuan Zhu
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Analytical Chemistry - Published
- 2023
3. Ultrasensitive detection of DNA methyltransferase activity: a novel dual-amplification fluorescence technique
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Qilin Wen, Dandan Li, Guidan Huang, Huai Xi, Hongcheng Pan, Lianming Zhang, Ziyuan Li, Xiaofen Xiao, and Wenyuan Zhu
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Spectrometry, Fluorescence ,Electrochemistry ,Environmental Chemistry ,Methyltransferases ,DNA ,Biosensing Techniques ,DNA Methylation ,Biochemistry ,DNA Modification Methylases ,Spectroscopy ,Analytical Chemistry - Abstract
DNA methyltransferase (MTase) is an important regulatory enzyme in various biological processes. However, current methods for investigating MTase activity are still limited in terms of sensitivity and/or generality. Herein, we proposed a dual amplification fluorescence strategy for the ultrasensitive detection of DNA adenine methylation methyltransferase (Dam MTase) activity based on strand displacement amplification (SDA) coupled with rolling circle amplification (RCA). In this study, the hairpin probe could not be cleaved by Nt.AlwI nicking endonuclease (Nt.AlwI) in the presence of Dam MTase, and the subsequent SDA-RCA reaction was blocked, resulting in a weak fluorescence signal. Moreover, the blocking effect was more pronounced at a higher concentration of Dam MTase. This assay provides a very low detection limit (down to 0.0067 U ml
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- 2022
4. Changes before and after COVID-19 pandemic on the personal hygiene behaviors and incidence of peritonitis in peritoneal-dialysis patients: a multi-center retrospective study
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Xiaofei Qin, Hu Yanglin, Xiao Hui Wang, Yanqiong Ding, Sheng Wan, Xiaofen Xiao, Fei Xiong, Li Xu, and Luo Qing
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Adult ,Male ,China ,medicine.medical_specialty ,Hand washing ,Urology ,medicine.medical_treatment ,Health Behavior ,Peritoneal dialysis ,030232 urology & nephrology ,Peritonitis ,030204 cardiovascular system & hematology ,Logistic regression ,Habits ,03 medical and health sciences ,0302 clinical medicine ,Personal hygiene ,Internal medicine ,medicine ,Humans ,Nephrology - Original Paper ,Risk factor ,Aged ,Retrospective Studies ,business.industry ,Incidence ,Incidence (epidemiology) ,Correction ,COVID-19 ,Hygiene ,Retrospective cohort study ,Middle Aged ,medicine.disease ,Logistic Models ,Nephrology ,Kidney Failure, Chronic ,Female ,business ,Hand hygiene - Abstract
Background The impact of Coronavirus disease (COVID-19) pandemic and its influence on personal hygiene behaviors and peritonitis rate in peritoneal-dialysis patients is unknown. Methods A multi-center retrospective study was conducted. We reviewed all the cases of peritoneal-dialysis (PD) patients from four major PD centers in Wuhan before and after COVID-19. There were 567 patients enrolled in total. Information was collected on personal hygiene behaviors, basic clinical characteristics, lab results, peritonitis details. We used Chi-square analysis to compare the personal hygiene behaviors, and used Chi-square goodness-of-fit analysis to compare the peritonitis rates before and after COVID-19. Multivariate logistic regression analysis was used to analyze the risk factors for peritonitis rate. Results There were no significant differences on peritonitis rates in six-month period before and after COVID-19 (p = 0.0756, Fig. 2 and Table 3). But Gram-positive infections decreased dramatically (p = 0.0041, Table 4). Personal hygiene behaviors such as length of time for washing hands when performing PD treatment, the frequency of washing hands before PD treatment and six general behaviors had significant differences (P
- Published
- 2021
5. Benzo[ a ]pyrene promotes progression in tongue squamous cell carcinoma
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Puping Liang, Linxuan Huang, Jianping Yu, Qingjian Chen, Yupeng Yao, Yan Zhang, and Xiaofen Xiao
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Genome instability ,Motility ,medicine.disease_cause ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Tongue ,Gentamicin protection assay ,Cell Movement ,Cell Line, Tumor ,Benzo(a)pyrene ,medicine ,Animals ,General Dentistry ,Carcinogen ,Cisplatin ,Mutation ,030206 dentistry ,Molecular biology ,Tongue Neoplasms ,Otorhinolaryngology ,chemistry ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Wound healing ,medicine.drug - Abstract
Objectives Benzo[a]pyrene (B[a]P) is a member of the polycyclic aromatic hydrocarbon (PAH) family. Although the potent carcinogenicity of high-dose B[a]P has been extensively reported, the effects of long-term exposure to B[a]P on the progression of tongue squamous cell carcinoma (TSCC) are poorly understood. Methods In the present study, TSCC cells were treated with 5 or 50 nM of B[a]P for three months. The proliferation and chemoresistance of B[a]P-treated cells to 5-fluorouracil or cisplatin were detected by CCK8. The motility of the B[a]P-treated cells was evaluated with wound healing analysis, invasion assay, and three-dimensional culture in decellularized mouse tongue matrix. Xenograft assay was used to investigate the aggressiveness of B[a]P-treated cells. Immunofluorescence staining, terminal restriction fragment assay, and whole-genome sequence were used to determine the mutation spectrums. Results Long-term 50 nM B[a]P-treated cells exhibited increased aggressiveness and chemoresistance to 5-fluorouracil or cisplatin. In addition, data from whole-genome sequencing demonstrated that C:T to A:T transitions were the predominant nucleotide substitutions occurred in 50 nM B[a]P-treated CAL27 cells. Furthermore, 102 non-synonymous or stop-gain mutations were enriched in the extracellular-matrix-receptor interactive pathway. Conclusions B[a]P exposure may contribute to genomic instability, and therefore, B[a]P may promote the progression of TSCC.
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- 2020
6. Combined nano/micro-structure of Ni12P5-Ni2P nanorod array for effective wide pH range HER and overall alkaline water-splitting
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Xiaofen Xiao, Chuangang Hu, Quanbin Dai, Chuanyin Xiong, Dong Liu, and Huile Jin
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General Chemical Engineering ,Electrochemistry ,Analytical Chemistry - Published
- 2022
7. Metagenomic Analysis Reveals
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Xiaofen, Xiao, Shandan, He, Fei, He, Xiaoyun, Wu, and Yuyan, Zheng
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Research Article - Abstract
The progression of early childhood caries (ECC) is caused by microbial colonized in dental plaque. However, the association framework both from 16s genus down to high resolution metagenomic strain level and from composition to genome function analysis on caries lacks. 16S rRNA sequence revealed the composition of 3–6 years dental caries (ECC, n = 29), and severe dental caries (SECC, n = 36) children are significantly different from caries-free controls (CF, n = 31). Especially, genus Neisseria is enriched in caries (P < 0.05). Metagenomics sequence of 3 ECCs, 3 SECCs, and 3 CFs reveals Neisseria bacilliformis ATCC BAA-1200 in genus Neisseria is also significantly enriched in caries (P < 0.05). Then, we recovered high-quality metagenomic assembly genomes (MAG), named bin 86, which have 99% identity with Neisseria bacilliformis ATCC BAA-1200 genome. Function analysis of Neisseria bacilliformis ATCC BAA-1200 genome shows its metabolism power of sugar and adhesion, colonization, acid production, and acid tolerance ability, which suggested Neisseria bacilliformis ATCC BAA-1200 may serve as a biomarker for childhood caries.
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- 2021
8. Metagenomic Analysis Reveals Neisseria bacilliformis Variation in the Early Childhood Caries Plaque Microbiome
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Shandan He, Xiaoyun Wu, Yuyan Zheng, Fei He, and Xiaofen Xiao
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Biology ,Dental plaque ,medicine.disease ,16S ribosomal RNA ,biology.organism_classification ,Genome ,Microbiology ,Other systems of medicine ,Complementary and alternative medicine ,Metagenomics ,medicine ,Neisseria bacilliformis ,Colonization ,Microbiome ,Early childhood caries ,RZ201-999 - Abstract
The progression of early childhood caries (ECC) is caused by microbial colonized in dental plaque. However, the association framework both from 16s genus down to high resolution metagenomic strain level and from composition to genome function analysis on caries lacks. 16S rRNA sequence revealed the composition of 3–6 years dental caries (ECC, n = 29), and severe dental caries (SECC, n = 36) children are significantly different from caries-free controls (CF, n = 31). Especially, genus Neisseria is enriched in caries ( P < 0.05 ). Metagenomics sequence of 3 ECCs, 3 SECCs, and 3 CFs reveals Neisseria bacilliformis ATCC BAA-1200 in genus Neisseria is also significantly enriched in caries ( P < 0.05 ). Then, we recovered high-quality metagenomic assembly genomes (MAG), named bin 86, which have 99% identity with Neisseria bacilliformis ATCC BAA-1200 genome. Function analysis of Neisseria bacilliformis ATCC BAA-1200 genome shows its metabolism power of sugar and adhesion, colonization, acid production, and acid tolerance ability, which suggested Neisseria bacilliformis ATCC BAA-1200 may serve as a biomarker for childhood caries.
- Published
- 2021
9. Clinical Features of Hemodialysis (HD) patients confirmed with Coronavirus Disease 2019 (COVID-19): a Retrospective Case-Control Study
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Duan Liu, Shanshan Han, Xin Zhang, Xiaohui Wang, Jing Li, Huan Zhou, Guosheng Yang, Xianhua Tan, Haibo Kuang, Ming-mei Li, Xiaofen Xiao, and Yong He
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medicine.medical_specialty ,biology ,business.industry ,medicine.medical_treatment ,Lymphocyte ,Case-control study ,Aspartate transaminase ,Gastroenterology ,medicine.anatomical_structure ,Internal medicine ,medicine ,biology.protein ,Alkaline phosphatase ,Hemodialysis ,Hemoglobin ,business ,Body mass index ,Dialysis - Abstract
BackgroundSince December 2019, Coronavirus Disease 2019(COVID-19) occurred in wuhan, China, and outbreaked rapidly into a global pandemic. This current poses great challenges to hemodialysis (HD) patients.ObjectiveTo make a comprehensive evaluation and comparison between HD patients confirmed with COVID-19 and the general HD patients.MethodsHD patients confirmed with COVID-19 in Wuhan No.5 Hospital were admitted as confirmed group from Jan 10 to Mar 15, 2020. And HD patients not infected in our dialysis center were chosen as control group. General characteristics, laboratory indicators were retrospectively collected, analyzed and compared.ResultsA total of 142 cases were admitted, including 43 cases in confirmed group and 99 in control group. Body mass index (BMI) was slightly lower in confirmed group than that in control group (P=0.011). The proportion of one or less underlying disease in confirmed group(51.16%) was higher than that in control group(14.14%)(P< 0.001), and the proportion of three or more underlying diseases in confirmed group(11.63%) was lower than that in control group(52.53%)(P< 0.001). Patients in confirmed group exhibited significantly lower hemoglobin, lymphocyte count, and lymphocyte percentage, but higher neutrophil percentage, neutrophil-to-lymphocyte ratio (NLR), C-reactive protein, aspartate transaminase, and alkaline phosphatase. There was no significant difference in age, gender, dialysis age, primary disease, the using of ACEI/ARB, platelet-to-lymphocyte ratio (PLR), and other indicators between the two groups.ConclusionsFaced with Severe Acute Respiratory Syndrome-CoV-2 (SARS-CoV-2), HD patients with lower BMI and hemoglobin were more susceptible to be infected, which might be related to malnutrition. Once confirmed with COVID-19, HD patients expressed obviously dis-regulated of inflammation and immune.
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- 2020
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10. Electrochemical assay for continuous monitoring of dynamic DNA methylation process
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Yuzhi Xu, Xiaoyong Zou, Zong Dai, Yingjun Ma, Li Zhang, Xiaofen Xiao, Jun Chen, and Danping Chen
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Models, Molecular ,Base pair ,Drug Evaluation, Preclinical ,Biomedical Engineering ,Biophysics ,Metal Nanoparticles ,Biosensing Techniques ,Biology ,010402 general chemistry ,01 natural sciences ,Catechin ,DNA Methyltransferase 3A ,Electron Transport ,chemistry.chemical_compound ,Electrochemistry ,Humans ,DNA (Cytosine-5-)-Methyltransferases ,Enzyme Inhibitors ,Detection limit ,Hybridization probe ,010401 analytical chemistry ,Electrochemical Techniques ,General Medicine ,Methylation ,DNA Methylation ,Molecular biology ,0104 chemical sciences ,Electrochemical gas sensor ,chemistry ,DNA methylation ,Gold ,Cytosine ,DNA ,Biotechnology - Abstract
A simple electrochemical strategy is reported for continuous monitoring of dynamic DNA methylation process over time. An electrochemical sensor was prepared by co-assembling of DNA probe and 6-mercapto-1-hexanol onto a gold electrode. The top of the DNA probe was labeled with 6-ferrocenylhexanethiol modified gold nanoparticle. The charge density between the C•G base pair was verified to be slightly reduced by DNA methylation, and could be further decelerated by ~ 25% upon co-locating a Br group onto methylated cytosine (mC). Therefore, in the presence of NaIO4/LiBr, the progressively methylated DNA on the sensor showed a clearly decreasing current over methylation time. The dynamic DNA methylation process was indicated continuously from the current decrease ratio, with a limit of detection of 0.0372µM. The strategy is convenient, cost-effective, and enable continuous profiling methylation process without distortion. Besides, the strategy was successfully applied for the studies on inhibitor screening and flanking sequence preference of DNA methyltransferase 3a. The results show that the activity of DNA methyltransferase 3a can be mildly inhibited by epigallocatechin gallate, and varies towards different flanking sequence with an order of 5'-CCGG-3' < 5'-CGCG-3' < 5'-CGCA-3'.
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- 2018
11. Correction to: Changes before and after COVID-19 pandemic on the personal hygiene behaviors and incidence of peritonitis in peritoneal-dialysis patients: a multi-center retrospective study
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Yanglin Hu, Li Xu, XiaoHui Wang, Xiaofei Jin, Sheng Wan, Qing Luo, Yanqiong Ding, Xiaofen Xiao, and Fei Xiong
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Nephrology ,Urology - Published
- 2021
12. A general approach to cobalt-based homobimetallic phosphide ultrathin nanosheets for highly efficient oxygen evolution in alkaline media
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Qiang Zhang, Zhongke Yuan, Chun-Ting He, Liming Dai, Jing Li, Shuangyin Wang, Xiaofen Xiao, Shenlong Zhao, Wensheng Lin, and Dingshan Yu
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Mass transport ,Materials science ,Renewable Energy, Sustainability and the Environment ,Phosphide ,Inorganic chemistry ,Oxygen evolution ,chemistry.chemical_element ,02 engineering and technology ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Pollution ,0104 chemical sciences ,chemistry.chemical_compound ,Nuclear Energy and Engineering ,chemistry ,Homogeneous ,Environmental Chemistry ,0210 nano-technology ,Porosity ,Bimetallic strip ,Cobalt - Abstract
A general and effective approach was proposed to fabricate a new family of Co-based bimetallic phosphide ultrathin nanosheets (CoM-P-NS, M = Ni, Mn, Cu, Zn) with homogeneous composition and unique porous architecture using ultrathin metal–organic framework nanosheets (MOFNs) as precursors for the first time, which yielded synergistically active sites, mass transport and dynamic modulations for the oxygen evolution reaction (OER). The optimized samples showed remarkable oxygen evolution activity in alkaline electrolytes, outperforming both the commercial RuO2 and Ir/C benchmarks and ranking the best among all the metal-phosphide electrocatalysts reported to date.
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- 2017
13. Graphene-based materials for polymer solar cells
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Zi-Yan Zhang, Wei Hong, Jing Li, Xudong Chen, Xiaofeng Lin, Dingshan Yu, Xiaofen Xiao, and Zhongke Yuan
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Materials science ,Graphene ,Graphene foam ,Energy conversion efficiency ,Nanotechnology ,02 engineering and technology ,General Chemistry ,Conductivity ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Polymer solar cell ,0104 chemical sciences ,law.invention ,law ,Thermal ,Electrode ,0210 nano-technology ,Graphene oxide paper - Abstract
Due to the remarkable electronic, optical, thermal, and mechanical properties, graphene-based materials have shown great potential in a wide range of technique applications. Particularly, the high transparency, conductivity, flexibility, and abundance make graphene materials highly attractive for polymer solar cells (PSCs). Graphene-based materials have been regarded as one promising candidate used in various parts in PSCs not only as electrodes, but also as interfacial layers and active layers with an aim to boost the power conversion efficiency of the devices. In this review, we summarize the recent progress about the design and synthesis of graphene-based materials for efficient PSCs along with the related challenges and future perspectives.
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- 2016
14. Self-Assembled Graphene Nanostructures and Their Applications
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Xiaofen Xiao, Zhongke Yuan, Dingshan Yu, and Quan Li
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Nanostructure ,Materials science ,Nanocomposite ,Graphene ,law ,Nanotechnology ,Electrocatalyst ,Energy storage ,Self assembled ,law.invention - Published
- 2018
15. ChemInform Abstract: Graphene-Based Materials for Polymer Solar Cells
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Xiaofen Xiao, Wei Hong, Xudong Chen, Jing Li, Xiaofeng Lin, Dingshan Yu, Zi-Yan Zhang, and Zhongke Yuan
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Graphene ,law ,Chemistry ,Electrode ,Energy conversion efficiency ,Thermal ,Energy transformation ,Nanotechnology ,General Medicine ,Conductivity ,Polymer solar cell ,law.invention - Abstract
Due to the remarkable electronic, optical, thermal, and mechanical properties, graphene-based materials have shown great potential in a wide range of technique applications. Particularly, the high transparency, conductivity, flexibility, and abundance make graphene materials highly attractive for polymer solar cells (PSCs). Graphene-based materials have been regarded as one promising candidate used in various parts in PSCs not only as electrodes, but also as interfacial layers and active layers with an aim to boost the power conversion efficiency of the devices. In this review, we summarize the recent progress about the design and synthesis of graphene-based materials for efficient PSCs along with the related challenges and future perspectives.
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- 2016
16. Self-Assembled Graphene-Based Architectures and Their Applications
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Zhe Zhao, Jing Li, Zhongke Yuan, Quan Li, Dingshan Yu, and Xiaofen Xiao
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energy conversion ,Nanostructure ,Materials science ,optoelectronics ,General Chemical Engineering ,Reviews ,General Physics and Astronomy ,Medicine (miscellaneous) ,Nanotechnology ,Review ,02 engineering and technology ,Related derivatives ,010402 general chemistry ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,law.invention ,Self assembled ,symbols.namesake ,law ,Energy transformation ,General Materials Science ,Electronics ,catalysis ,energy storage ,Graphene ,General Engineering ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrostatic attraction ,self‐assembled graphene ,symbols ,van der Waals force ,0210 nano-technology ,hybrid structures - Abstract
Due to unique planar structures and remarkable thermal, electronic, and mechanical properties, chemically modified graphenes (CMGs) such as graphene oxides, reduced graphene oxides, and the related derivatives are recognized as the attractive building blocks for “bottom‐up” nanotechnology, while self‐assembly of CMGs has emerged as one of the most promising approaches to construct advanced functional materials/systems based on graphene. By virtue of a variety of noncovalent forces like hydrogen bonding, van der Waals interaction, metal‐to‐ligand bonds, electrostatic attraction, hydrophobic–hydrophilic interactions, and π–π interactions, the CMGs bearing various functional groups are highly desirable for the assemblies with themselves and a variety of organic and/or inorganic species which can yield various hierarchical nanostructures and macroscopic composites endowed with unique structures, properties, and functions for widespread technological applications such as electronics, optoelectronics, electrocatalysis/photocatalysis, environment, and energy storage and conversion. In this review, significant recent advances concerning the self‐assembly of CMGs are summarized, and the broad applications of self‐assembled graphene‐based materials as well as some future opportunities and challenges in this vibrant area are elucidated.
- Published
- 2017
17. Chemical-oxidation cleavage triggered isothermal exponential amplification reaction for attomole gene-specific methylation analysis
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Xiaoyong Zou, Yuzhi Xu, Wenyuan Zhu, Zong Dai, Xiaofen Xiao, and Chang Niu
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Molecular Sequence Data ,Cleavage (embryo) ,Fluorescence ,Mass Spectrometry ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,law ,Limit of Detection ,Gene expression ,Electrochemistry ,Humans ,DNA Cleavage ,Organic Chemicals ,Polymerase chain reaction ,Base Sequence ,Methylation ,Nucleic acid amplification technique ,DNA ,DNA Methylation ,Molecular biology ,Kinetics ,chemistry ,DNA methylation ,Nucleic Acid Amplification Techniques ,Oxidation-Reduction ,Cytosine - Abstract
Genomic 5-methylcytosine (5-mC) modification is known to extensively regulate gene expression. The sensitive and convenient analysis of gene-specific methylation is wishful but challenging due to the lack of means that can sensitively and sequence-selectively discriminate 5-mC from cytosine without the need for polymerase chain reaction. Here we report a chemical-oxidation cleavage triggered exponential amplification reaction (EXPAR) method named COEXPAR for gene-specific methylation analysis. EXPAR was proved to not only have rapid amplification kinetics under isothermal condition but also show excellent sequence-selectivity and linear-dependence on EXPAR trigger. Further initiation of EXPAR by chemical-cleavage of DNA at 5-mC, the COEXPAR showed high specificity for methylated and nonmethylated DNA, and ∼10(7) copies of triggers were replicated in 20 min, which were used to quantify the methylation level at the methylation loci. As a result, the gene-specific methylation level of a p53 gene fragment, as a target model, was analyzed in two linear ranges of 10 fM-1 pM and 1 pM-10 nM, and limits of detection of 411 aM (S/N = 3) by fluorescence, and 576 aM (S/N = 3) by electrochemistry. The method fulfilled the assay in an isothermal way in ∼5 h without the need for tedious sample preparation and accurate thermocycling equipment, which is likely to be a facile and ultrasensitive way for gene-specific methylation analysis.
- Published
- 2015
18. Cage-Confinement Pyrolysis Route to Ultrasmall Tungsten Carbide Nanoparticles for Efficient Electrocatalytic Hydrogen Evolution.
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Yan-Tong Xu, Xiaofen Xiao, Zi-Ming Ye, Shenlong Zhao, Rongan Shen, Chun-Ting He, Jie-Peng Zhang, YadongLi, and Xiao-Ming Chen
- Subjects
- *
PYROLYSIS , *TUNGSTEN carbide , *HYDROGEN evolution reactions - Abstract
The size-controlled synthesis of ultrasmall metal-based catalysts is of vital importance for chemical conversion technologies. Here, a cage-confinement pyrolysis strategy is presented for the synthesis of ultrasmall tungsten carbide nanoclusters/nanoparticles. An RHO type zeolitic metal azolate framework MAF-6, possessing large nanocages and small apertures, is selected to confine the metal source W(CO)6. High temperature pyrolysis gives tungsten carbide nanoclusters/nanoparticles with sizes ca. 2 nm, which can serve as an excellent electrocatalyst for the hydrogen evolution reaction. In 0.5 M H2SO4, it exhibits very low overpotential of 51 mV at 10 mA cm-2 and Tafel slope of 49 mV per decade, as well as the highest exchange current density of 2.4 mA cm-2 among all tungsten/molybdenum-based catalysts. Moreover, it also shows excellent stability and antiaggregation behavior after long-term electrolytic process. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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19. Chemical-Oxidation Cleavage Triggered Isothermal Exponential Amplification Reaction for A ttomole Gene-Specific Methylation Analysis.
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Yuzhi Xu, Chang Niu, Xiaofen Xiao, Wenyuan Zhu, Zong Dai, and Xiaoyong Zou
- Published
- 2015
- Full Text
- View/download PDF
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