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The spread of IMP-type MBLs has increased dramatically in recent years. We discovered three novel IMP variants from three clinical isolates in China.

Objectives To dissect genomic features of IncHI3 plasmids and their derivatives sharing the IncHI3 core backbone. Methods An extensive genomic comparison was applied to all 20 available sequenced IncHI3 plasmids and their derivatives, including eight sequenced here and another 12 from GenBank. Results There were 20 IncHI3 plasmids and their derivatives, including 11 IncHI3 plasmids that contained single-replicon (repHI3B), double-replicons (repHI3B + repBIncFIB−6.1), and triple-replicons (repHI3B + repBIncFIB−6.1+repBIncFIB−8.1) and nine IncHI3 plasmid derivatives that were composed of the same IncHI3 backbone (including tra1 and tra2, and parABC) but different replication gene profiles instead of repHI3B. Based on the replication gene profiles, IncFIB-6.1, IncFIB-8.1, and IncFIB-6.1:8.1 plasmids were newly designated from derivatives. The 20 IncHI3 plasmids and their derivatives displayed massive gene acquisition and loss across the genomes and multiple accessory modules, including 13 Tn1696 derivatives, five MDR regions, two iuc regions, and 11 other key accessory regions. Two novel AGEs: In1765 and Tn7066, were first reported in this study. Conclusions Data presented here provides the evolution history of IncHI3 plasmids and their derivatives and a deeper insight into genomic diversification.

Energy-saving performance of green technology for building projects has become the research hot spot. Using three reference simulation models of multi-story residential buildings designed located in Guangzhou city with hot summer and warm winter, this study simulated and identified critical energy-influencing factors in a traditional design. Green (renovation) technologies design were presented to enhance the conventional technique's energy-saving performances. DesignBuilder software, Pareto rule, and assessment index were used to analyze and identify the proper green technology for multi-story residential buildings in Guangzhou city. The results show that residential buildings' energy consumption in Guangzhou city mainly comes from five critical factors. The energy-saving performance from changing an envelope structure measures is the largest, reaching total sensitivities of 52.95% for 4-story frame structure, 54.06% for 3-story frame structure, and 60.59% for 2-story frame structure and reaching 16.14% of the total contribution for 4-story frame structure, 16.76% for 3-story frame structure, and 17.89% for 2-story frame structure, respectively. Based on this study, the managerial implications for decision-makers are presented. In a green energy-saving renovation technologies design of multi-story residential buildings in Guangzhou city with hot summer and warm winter, priority should be given to the passive technologies. Then enhance the energy-saving performance combined with appropriate active technologies. This case study research results guide decision-makers to improve multi-story residential buildings' energy-saving performance in similar regions with hot summer and warm winter.

Energy efficient building projects has become the focus of sustainable development. In this article, 2 indexes, energy-saving sensitivity and energy-saving contribution, are proposed to assess the energy efficiency of residential buildings. Four green measures to improve the energy efficiency of residential building with either passive or active technologies are designed and their saving performances are evaluated with the case study of a residential building simulation model in Changchun, region in China using the 2 indexes by simulations in the Designbuilder simulation software. The results reveal that improving the energy efficiency of residential buildings in severely cold regions should be focused on two key energy consumption factors, A1-heating (gas) and A2-zone sensible heating. Green measures M4-modifying the heating ventilating and air conditioning system and M1-improving envelope structures are found with high energy-saving sensitivities on the energy consumption factors A1 and A2. The energy-saving contribution of green measures M4 is the largest with the value of 80.17%, and that of green measure M2-adding shading facilities is the smallest with the negative value of − 8.53%. These results suggest that M4 and M1 are the effective green energy-saving renovation measures of the residential buildings in severely cold regions. The assessment method and simulation case study results provide a theoretical foundation and practical suggestions for the decision making to effectively reduce the energy consumption of residential buildings in severely cold regions by adopting appropriate green measures.

Jiayao Guan,1,* Chunmei Bao,2,* Peng Wang,3 Ying Jing,3 Lingling Wang,3 Xinyue Li,3 Xiaofei Mu,3 Boan Li,2 Dongsheng Zhou,3 Xuejun Guo,1 Zhe Yin3 1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, 130122, People’s Republic of China; 2Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100039, People’s Republic of China; 3State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhe Yin, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People’s Republic of China, Tel +86-10-66948557, Email jerry9yin@163.com Xuejun Guo, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, 130122, People’s Republic of China, Tel +86-431-86985931, Email xuejung2021@163.comPurpose: The aim of this study was to gain a deeper genomics and bioinformatics understanding of diversification of accessory genetic elements (AGEs) in Providencia.Methods: Herein, the complete genome sequences of five Providencia isolates from China were determined, and seven AGEs were identified from the chromosomes. Detailed genetic dissection and sequence comparison were applied to these seven AGEs, together with additional 10 chromosomal ones from GenBank (nine of them came from Providencia).Results: These 17 AGEs were divided into four groups: Tn 6512 and its six derivatives, Tn 6872 and its two derivatives, Tn 6875 and its one derivative, and Tn 7 and its four derivatives. These AGEs display high-level diversification in modular structures that had complex mosaic natures, and particularly different multidrug resistance (MDR) regions were presented in these AGEs. At least 52 drug resistance genes, involved in resistance to 15 different categories of antimicrobials and heavy metal, were found in 15 of these 17 AGEs.Conclusion: Integration of these AGEs into the Providencia chromosomes would contribute to the accumulation and distribution of drug resistance genes and enhance the ability of Providencia isolates to survive under drug selection pressure.Keywords: Providencia, integrative and conjugative elements, integrative and mobilizable elements, unit transposons, multidrug resistance

This study systematically analyzed the strategies and approaches for the sustainable development of green buildings (SDGB) and summarized potential driving forces of SDGB from four aspects of green buildings (GB), including market development environment, economic value, the degree of social participation, and ecological value. A structural equation modelling (SEM) technique was used to explore dynamic interactions and leading roles of driving forces of SDGB based on data collected from a questionnaires survey of 240 respondents. Based on the SEM technique, a driving structural equation model was constructed to reveal the key driving paths and forces of SDGB. SPSS (Statistical Package for Social Sciences) and AMOS (Advanced MOrtar System) were applied to analyze the data. A dynamic system of SDGB was established to explore the SDGB’s driving mechanism. The results show that the market development environment and ecological value have a significant direct and comprehensive impact on SDGB. The economic value and the degree of social participation are key path nodes of SDGB. This study may provide appropriate strategies and practical guidance for better promoting SDGB.

Background: Many pairwise meta-analyses (MAs) related to therapies of varicose veins have been published, but their reporting and methodological quality remain unclear. The present study was designed to assess the overall quality of pairwise MAs related to therapies of varicose veins. Methods: We will systematically search 4 electronic databases, including PubMed, EMBASE, Cochrane Library, Chinese Biomedical Database, to identify pairwise MAs related to therapies of varicose veins. The search time-span was set from inception to March 2019. The pairwise MAs related to therapies of varicose veins will be included in our overview. The reporting and methodological quality of included MAs will be assessed using preferred reporting items for systematic review and meta-analysis and a measurement tool to assess systematic reviews 2, respectively. Meanwhile, we will extract some general characteristics of included MAs, including first author; published year, journal, sample size, number of studies, number of randomized controlled trials and intervention details, and so on. All literatures screening, quality assessment, and data extraction will be independently completed by 2 of all reviewers, and any disagreement will be resolved by discussion. Besides, an increasingly popular method – evidence mapping, will be used to present the whole evidence landscape related to therapies of varicose veins. The assessment results will be presented as percentage and event/total. The Excel 2016 will be used to manage and analyze data. Results: The results of the overview will be submitted to a peer-reviewed journal for publication. Conclusion: This overview will summarize the overall reporting and methodological quality related to therapies of varicose veins. PROSPERO registration number: CRD42019126722.

A novel economic analyzing method was proposed to evaluate the combined cooling, heating and power (CCHP) system based on energy cascade utilization and the current feed-in tariff policy in China. Natural gas consumption rates were chosen as the economic criteria to evaluate operation costs of cooling, heating and electricity of an office building in Changchun, Jilin of China. Based on the building’s energy consumption and current energy price structures, the following electric load (FEL) operation strategy was used to achieve a more profitable performance from the CCHP system. Because of the deficiency in economic performance, no excess electricity was generated or sold to the grid, even though selling electricity to the grid was allowed when certain restrictions were satisfied. Meanwhile, the power generation unit size was optimized according to the basic electric requirement of the building. Additionally, a water storage tank was used because it could improve the primary energy consumption ratio and the profitability of the CCHP system.

The traditional unmanned transmission line inspection airship relies on the deflection of the tail to realize the turning, the turning radius is large, the U-turn is difficult, and hovering in the air may not rotate 360 degrees fixed point, which is not conducive to the power line inspection of the hidden points or suspected fault points of the key observation work. This paper introduces a new type of transmission line of unmanned airship, which uses Y tail steering driving mechanism, the new unmanned transmission line inspection airship relies on the propeller and steering auxiliary flight, high stability and steering. The hover U-turn more flexible to control the technical requirements are relatively simple, and can reduce the size of the tail, reducing the probability of failure airship. Through the line patrol test of the transmission line in Dandong, it is proved that the unmanned airship designed in this paper has good low-speed maneuverability and turning performance.

Rationale and Objectives The aim of this study was to preliminarily investigate whether an enlarged geniculate ganglion fossa (GGF) on temporal bone computed tomography can diagnose GGF fracture in patients with traumatic facial paralysis by evaluating the diameter of the GGF. Materials and Methods Thirty-six patients who underwent computed tomography before confirmation of GGF fracture on otologic surgery were recruited into a study group. Additionally, a cohort of 107 patients with no histories of head trauma, no structural abnormalities of inner ear, and no clinical symptoms of facial nerve disability who underwent computed tomography for other reasons were selected as a control group. The diameters of the GGFs of the study group were evaluated by two observers and compared retrospectively with those of the control group. Wilcoxon's test was used to compare discrepancies of both sides, and intraclass correlation coefficients were used to evaluate intraobserver and interobserver reliability. Results The measurement of diameters showed good interobserver and intraobserver consistency. The discrepancy in the measurement of transdiameter between both sides of the GGF on reformatted transverse images of the study group was significantly different from that of the control group (Wilcoxon's test, P P Conclusions An enlarged GGF on temporal bone computed tomography offers an additional sign for the diagnosis of GGF fracture in patients with traumatic facial paralysis.

The aim of this study was to preliminarily investigate whether an enlarged geniculate ganglion fossa (GGF) on temporal bone computed tomography can diagnose GGF fracture in patients with traumatic facial paralysis by evaluating the diameter of the GGF.Thirty-six patients who underwent computed tomography before confirmation of GGF fracture on otologic surgery were recruited into a study group. Additionally, a cohort of 107 patients with no histories of head trauma, no structural abnormalities of inner ear, and no clinical symptoms of facial nerve disability who underwent computed tomography for other reasons were selected as a control group. The diameters of the GGFs of the study group were evaluated by two observers and compared retrospectively with those of the control group. Wilcoxon's test was used to compare discrepancies of both sides, and intraclass correlation coefficients were used to evaluate intraobserver and interobserver reliability.The measurement of diameters showed good interobserver and intraobserver consistency. The discrepancy in the measurement of transdiameter between both sides of the GGF on reformatted transverse images of the study group was significantly different from that of the control group (Wilcoxon's test, P.001). Discrepancy in the GGF on transverse images of the study group was larger than that of the control group. A significant difference existed in the discrepancy in vertical diameter between the study and control groups (Wilcoxon's test, P.001) as well.An enlarged GGF on temporal bone computed tomography offers an additional sign for the diagnosis of GGF fracture in patients with traumatic facial paralysis.