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Numerical simulation and theoretical prediction show that the observed exponential decrease of the a.f. noise frequency with sampling depth in an ICP source result from a coupling effect of vortex dissipation both at torch outlet and sampling gap.

Objective: To investigate the effect of storage duration of suspended red blood cells (SRBC) before intraoperative infusion on coagulation indexes, routine blood examination and immune function in patients with gastrointestinal (GI) tumors. Methods: We divided clinical data of one hundred patients with GI tumors who underwent surgical treatment in our hospital into two different groups according to the storage duration of SRBC use for intraoperative infusion. The short-term group (n=50) had patients with SRBC storage durations shorter than two weeks, and the long-term group (n=50) had patients with storage durations longer than two weeks. We compared the coagulation, immune function, routine blood profile, electrolyte levels and adverse reactions assessment results between the two groups. Results: Compared with before transfusions, the levels of fibrinogen (FIB) and activated partial prothrombin time (APTT) after blood transfusions were higher than those before transfusion (P

The uncertainty of the energy measurement of the electron beam on circular electron positron collider (CEPC) must be smaller than 10$\mathrm{MeV}$ to make sure the accurate measurement of the mass of the Higgs boson. In order to simplify the energy measurement system, a new method is proposed by fitting the Compton edge of the energy distribution of the gamma ray from a microwave-electron Compton scattering. With our method, the uncertainty of the energy measurement is 6$\mathrm{MeV}$ for the electron energy of $120\mathrm{GeV}$ in the Higgs mode. In this system, the energy resolution of the gamma detection needs to reach $10^{-4}$. Therefore, only the high-purity germanium (HPGe) detector can meet the critical requirement. In a head-on collision mode, the initial photons should be microwave photons with the wavelength of 3.04 centimeters. A cylindrical resonant cavity with selected ${TM_{010}}$ mode is used to transmit microwaves. After the microwave-electron Compton backscattering, the scattered photons and the synchrotron-radiation background transmit a shielding structure and then are detected by a HPGe detector at the end of the beam line of the synchrotron-radiation applications. The hole radius in the side wall of the cavity is about $1.5\mathrm{mm}$ to allow the electron beam passing through. The results of the computer simulation technology (CST) software shows that the influence of the hole radius on the cavity field is negligible. The change of the resonance frequency can be easily corrected by fine-tuning the cavity size., Comment: 22 pages, 12 figures

The new scheme of the energy measurement of the extremely high energy electron beam with the inverse Compton scattering between electrons and microwave photons requires the precise calculation of the interaction cross section of electrons and microwave photons in a resonant cavity. In the local space of the cavity, the electromagnetic field is expressed by Bessel functions. Although Bessel functions can form a complete set of orthogonal basis, it is difficult to quantify them directly as fundamental wave functions. Fortunately, with the Fourier expansion of Bessel functions, the local electromagnetic field can be considered as the superposition of a series of plane waves. Therefore, with corresponding corrections of the cross section formula of the classical Compton scattering, the cross section of the linear or nonlinear microwave Compton scattering in the local space can be described accurately. As an important application of our results in astrophysics, corresponding ground verification devices can be designed to perform experimental verifications on the prediction of the Sunyaev-Zeldovich (SZ) effect of the cosmic microwave background radiation. Our results could also provide a new way to generate wave sources with strong practical value, such as the terahertz waves, the ultra-violet (EUV) waves, or the mid-infrared beams., Comment: 16 pages, 5 figures

In this study, eggs from free-range and barn chickens in farms around a fluorochemical facility were collected to assess the distribution profiles of perfluoroalkyl acids (PFAAs), including isomers of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and perfluorohexane sulfonate (PFHxS), in egg yolk and albumen. The results revealed that the concentrations of PFAAs in yolks were significantly higher than those in albumen. All 17 PFAAs examined could be detected in yolks, showing decreasing concentrations with increasing distance from the fluorochemical facility. The three predominant compounds in yolks were perfluorobutanoic acid (PFBA, mean concentration 81.4 ng/g ww), PFOS (28.0 ng/g ww), and PFOA (4.83 ng/g ww), and this result is consistent with the product structure of the facility. Moreover, n-PFOA, n-PFOS, and n-PFHxS were the dominant contaminants in yolk, with mean concentrations of 4.75, 25.7, and 4.29 ng/g ww, respectively. In albumen, PFBA was still the predominant PFAA congener (mean concentration = 3.93 ng/g ww), followed by PFOA. Docking analysis indicated that the PFAAs presented higher binding abilities with the low density lipoprotein, high density lipoprotein, and vitellin proteins in yolk than that with ovalbumin albumen proteins, which might be the main factor influencing the possible difference in distributions of PFAAs in yolk and albumen.

Recent experimental data for anomalous magnetic moments strongly indicates the existence of new physics beyond the Standard Model. Energetic μ + bunches are relevant to μ + rare decay, spin rotation, resonance and relaxation (μSR) technology, future muon colliders, and neutrino factories. In this paper, we propose prompt μ + acceleration in a nonlinear toroidal wakefield driven by a shaped steep-rising-front Laguerre–Gaussian (LG) laser pulse. An analytical model is described, which shows that a μ + beam can be focused by an electron cylinder at the centerline of a toroidal bubble and accelerated by the front part of the longitudinal wakefield. A shaped LG laser with a short rise time can push plasma electrons, generating a higher-density electron sheath at the front of the bubble, which can enhance the acceleration field. The acceleration field driven by the shaped steep-rising-front LG laser pulse is about four times greater than that driven by a normal LG laser pulse. Our simulation results show that a 300 MeV μ + bunch can be accelerated to 2 GeV and its transverse size is focused from an initial value of w 0 = 5 μm to w = 2 μm in the toroidal bubble driven by the shaped steep-rising-front LG laser pulse with a normalized amplitude of a = 22.

Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor (GECAM) is a pair of small satellites designed to monitor the Gamma-Ray Bursts (GRBs), some of them are possibly associated with Gravitational Wave Events (GWE). Each satellite includes twenty-five Gamma-Ray Detectors (GRDs) and eight Charged Particle Detectors (CPDs). GRDs are used to detect X-rays and gamma-rays to realize the observation and location of significant events such as GRBs or other X-ray Bursts (XRBs). Meanwhile, CPDs are used to help GRDs distinguish between GRBs and charged particle events (the majority are electrons) in space. In this paper, we present the electron calibration results of the CPDs, including the energy linearity, energy resolution, angular response, detection efficiency and response in high count rates. Moreover, we use the calibration spectra to verify and correct the mass model of CPD in Geant4 simulation. After the correction, the simulated spectra are in good agreement with the measurements.

Crop rotation can increase crop productivity and suppress disease. However, current understanding of responses of soil functional microbial communities to agricultural management practices is limited. Here, we conducted a field experiment to investigate the impact of crop rotations, fertilization regimens, and wheat variety on rhizosphere functional communities involved in phosphorus (P) cycling. Rotation altered the composition of phoD-harboring bacteria in the wheat rhizosphere. The rare taxa of Roseivivax was enriched in the Soybean (Glycine max (L.) Merr.)-Wheat (Triticum aestivum L.) (SW) rotation, whereas Hydrogenophaga was enriched in the Maize (Zea mays L.) -Wheat (Triticum aestivum L.) (MW) rotation. However, fertilization regimens (high and low N, P and K fertilizers) in the soybean/maize season and wheat variety (Jimai 22 and Shannong 28) had no impact. SW rotation increased phoD gene abundance, and the latter was significantly negatively correlated to available phosphorus (AP) content. Hydrogenophaga was positively with wheat biomass (p

To explore the molecular basis of an individual with Bel variant of the ABO blood group.The ABO antigen and serum antibody of the individual were detected by serological method. All coding regions and flanking introns of the ABO gene were amplified with PCR and sequenced bidirectionally. The haplotypes of the individual were analyzed by cloning and sequencing. A three dimensional model of the mutant protein was constructed and analyzed.The individual has expressed a very weak B antigen on its red blood cells by absorption and elution testing, which was identified as a Bel variant phenotype. The heterozygous sites in exon 6 (261del/G) and exon 7 (297A/G, 484del/G, 526C/G, 657C/T, 703G/A, 796C/A, 803G/C, 930G/A) of the coding region of the ABO gene were identified by direct sequencing. Haplotype analysis showed that the individual has carried an O01 allele and a novel B allele. The sequence of the novel B allele was identical to B101 except for a del G at nucleotide position 484 (484delG), which was nominated as B120 by the Blood Group Antigen Gene Mutation Database (dbRBC NCBI). The 484delG mutation of the B allele has led to a reading frame shift and created a premature terminal codon for the glycosyltransferase (GT) enzyme. Prediction of the 3D structure suggested that the GT enzyme has become an incomplete protein only with its N-terminal region.The 484delG mutation of the glycosyltransferase B gene has probably abolished or reduced the enzymatic activity and resulted in the Bel variant phenotype.

To study the serological characteristics and molecular mechanism for a rare Pk phenotype of the P1Pk blood group system.The blood group of the proband was identified by serological techniques. The coding region and flanking intronic sequences of the β-1,3-N-acetylgalactosyltransferase gene (B3GALANT1) associated with the Pk phenotype were analyzed using polymerase chain reaction sequence-based typing.The proband was identified as having a rare Pk phenotype including anti-P in her serum. The blood group of her daughter and husband showed a P2 phenotype. The nucleotide sequences of the B3GALANT1 gene of her husband and two randomly-chosen individuals were the same as the reference sequence (GenBank AB050855). Nucleotide position 433 CT homozygous mutation in the B3GALANT1 was found in the proband, which has resulted in a stop codon at amino acid position 145, which may produce a premature protein capable of decreasing or inhibiting the activity of the β -1,3-N-acetylgalactosyltransferase. The nucleotide position 433 C/T heterozygous in the B3GALANT1 was found in her daughter.The Pk phenotype resulted from 433 CT mutation in the B3GALANT1 gene has been identified.

Precipitation of gold nanoparticles is achieved in silicate glass doped with Au ions by UV femtosecond laser irradiation and subsequent annealing treatment. The influences of different power and annealing temperature on the formation of Au nanoparticles are studied: the higher the laser power, the larger the diameter of Au nanoparticles. As the annealing temperatures increase, the colors of the samples are from light purple to purple-red. The peaks of absorption spectrum of the nanoparticles move to the long wavelength.

To explore the molecular basis for an individual with rare p phenotype in the P1Pk blood group system.Erythrocyte blood group antigens and antibodies in serum were identified in the proband and five family members with a serological method. Coding regions and flanking untranslated regions of the α1,4-galactosyltransferase gene (A4GALT) encoding P1Pk antigens were amplified with polymerase chain reaction and directly sequenced. The haplotypes of A4GALT in the parents of the proband were also analyzed by cloning sequencing.The proband was found with a rare p phenotype with anti-Tja antibody in his serum by serological method. The other family members all had a common P2 phenotype. The results of DNA sequencing showed that a cytosine was inserted at nucleotide position 1026 to 1029 (1026_1029insC) of both alleles of the A4GALT gene in the proband. The mutation has caused a reading frame shift and formed a mutant protein by extending 92 amino acid residues. The other family members were either heterozygous for the insertion or of the wild type at above position.The 1026_1029insC mutation of the A4GALT gene is probably responsible for the p phenotype identified for the first time in Chinese population. The individual with the p phenotype possesses anti-Tja antibody.

To analyze specific expression of blood group genes using nucleated erythroid cells cultured from un-mobilized peripheral stem cells in vitro.Hematopoietic stem cells(HSC) bearing the CD34 antigen were isolated from peripheral blood by centrifugation and magnetic beads sorting, followed by suspension culture in vitro. Cells were collected from medium on various stages and analyzed by immunofluorescence. The RNA transcription of RH and ABO blood group genes was analyzed using culture cells on day 12.A total of(3.19±0.13) ×10 (4) CD34+cells were isolated from about 50 mL peripheral blood with a recovery rate of 67.3%±2.7%. The cells amount in erythroid-lineage culture system on day 9 reached a plateau of a 237.1±15.5-fold amplification of the initial cell input. The stem cell-specific CD34 antigen was dropped off, while the erythroid-specific CD235a and CD240D antigens were increased in culture period. RHD/CE and ABO genes can be amplified using RNA extracted from culture cells on day 12, and genotypes of Rh and ABO systems by DNA sequencing were consistent with their serologic phenotypes.A method was established to analyze the gene expression of erythroid blood group derived from un-mobilized peripheral stem cells cultured in vitro. It can be used to study the expression of various erythroid-specific genes.

The relationship between CD36 expression level in platelets and polymorphism of the CD36 gene still needs to be explored. Here, we investigated polymorphisms of the CD36 gene and CD36 expression level in platelets in the Chinese Han population.A total of 477 samples were sequenced for exons 2 to 14 of the CD36 gene using a polymerase chain reaction sequence-based typing method. In 192 of these individuals the expression levels of CD36 antigen were analysed by flow cytometry. The genotype-phenotype relationship in platelets was analysed.A total of 22 variants of the CD36 gene were identified, of which five variants (111 AT, 681 CA, 1172-1183 del12b, 1236 delT and 1395 AC) were novel variations, and nine were also found in single nucleotide polymorphism database (dbSNP) but had not been confirmed in individuals with CD36 deficiency. Two variants (329-332 delAC and 1228-1239 del12bp) in the coding region are the most frequent mutations in the Chinese population. Type II CD36 deficiency was identified in seven of 192 individuals, giving a frequency of 3.6%. Individuals with CD36 variations or wild-type genotypes both showed CD36 antigen negative, low-level and high-level expression patterns in platelets. The frequency of the nt-132 AC polymorphism in the 5'-UTR is relatively high in the Chinese population (0.3516): the expression of CD36 was lower in individuals with nt-132 AC than in those with the wild-type genotype.The distribution of CD36 gene variants in the Chinese population is different from that previously reported. The levels of expression of CD36 antigen in platelets are not determined directly by the genotypes of the CD36 coding region. This suggests that the molecular basis of type II CD36 deficiency may be derived from combined effects of coding region and potential cis-regulatory elements in the 5'-UTR of the CD36 gene.

To delineate serological features and genetic basis for a rare p phenotype of P1Pk blood group system found in a Chinese individual.Serological assaying was carried out for a proband with unexpected antibody found in his serum using specific antibodies and panel cells. Coding regions and flanking introns of α 1,4-galactosyltransferase gene (A4GALT) associated with the p phenotype were screened with polymerase chain reaction and DNA sequencing.A rare p phenotype of the P1Pk blood group system has been identified with red blood cells from the proband, whose serum contained anti-Tja antibody which can agglutinate and hemolyze with other common red blood cells. Other members of the proband's family were all normal with P1 or P2 phenotype. DNA sequencing has identified in the proband a homozygous 26 bp deletion at position 972 to 997 of the A4GALT gene. The deletion has caused a shift of the reading frame, resulting in a variant polypeptide chain with additional 83 amino acid residues compared with the wild-type protein. Other family members were either heterozygous for above deletion or non-deleted.A 26 bp deletion at position 972 to 997 of the A4GALT gene has been identified in a Chinese individual with p phenotype.

To well describe the Ti(IV)-catalyzed H 2 O 2 /O 3 reaction in aqueous solution, a kinetic model was established based on its mechanism. This model was then validated by the experiments of acetic acid degradation in aqueous solution. It was found that the correlation coefficient of fittings was higher than 0.970. Three key operating factors affecting organic degradation in the Ti(IV)-catalyzed H 2 O 2 /O 3 process were studied, including Ti(IV) concentration, dissolved ozone concentration and initial H 2 O 2 concentration. Furthermore, some experiments were conducted to determine the rate constant for dissolved ozone decomposition initiated by Ti 2 O 5 2+ . The rate constant measured is almost in accord with the data analyzed by this kinetic model. The goodness of fittings demonstrated that this model could well describe the kinetics of the Ti(IV)-catalyzed H 2 O 2 /O 3 reaction mathematically and chemically. Therefore, this kinetic model can provide some useful information to optimize the parameters in ozonation of water containing certain pollutants.

Background Chimerism is the presence of two or more genetically distinct cell populations in one organism. Here, we reported the identification of dispermic chimerism in a 25-year-old male. Methods Blood grouping was performed with standard gel centrifugation test cards. ABO and HLA-A,-B,-C,-DRB1 and -DQB1 loci genotyping was determined with PCR sequence-based typing. A quantitative analysis of dual red cells populations was measured by flow cytometer. The karyotype was analyzed by G-banded chromosomes. Short tandem repeat (STR) analysis was performed on blood, buccal mucosal and hair shafts samples. Results A mixed-field agglutination with anti-B antibody was observed with gel centrifugation tests, which showed a double populations of O and B groups RBCs. Two groups RBCs were also observed by flow cytometer with nearly 90% O group cells and 10% B group cells. The normal O01, O02, B101 alleles were identified in DNA sample of the proband. STR analysis revealed three alleles for D8S1179,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,TPOX and D18S51 loci. HLA-DRB1 and -DQB1 loci had three alleles and a karyotypic mosaic was found with 60% 46, XY and 40% 46, XX karyotype in the proband. In all studies, the third allele was attributable to a dual paternal contribution. Conclusion A individual with dispermic chimerism was identified, which would generate by fertilization of an oocyte and the corresponding second polar body by two different sperms.

Background A2 phenotype is a common subgroup of blood group A, but the serological characteristic and genetics basis of A2 phenotype currently was rare reported in the Chinese Han population. Here, a large scale study of the serology and genetics of A2 and A2B phenotypes was performed. Methods/materials 11263 Chinese individuals with group A and AB phenotypes were determined for A2 antigen with the standard serological method. The full coding region of the ABO gene was sequenced in the individuals with A2 and A2B phenotypes. Some samples including each ABO genotypes were chosen for determining the activity of glycosyltransferase A (GTA) in plasma. Results 134 individuals were assigned as A2 and A2B phenotypes in 11263 individuals. There was imbalance in A2 and A2B phenotypes and the proportion of A2B among AB samples was significantly higher than that of A2 in group A samples. All samples of the A2 and A2B phenotypes were classified into A2-related allele group, A1-related allele group and the other group based on kind of the ABO genotype. Four novel A2-related alleles (A217, A218, A219, A220) were identified. The individuals with same genotype showed different agglutination strength with anti-A1 and anti-H on their RBCs. The plasma from individuals with A2-related allele had almost no GTA activity, while plasma from individuals with A1-related allele had some GTA activity. Conclusion A2 and A2B phenotypes could derive from different genotypes and the serological characteristic may be heterogeneity in the Chinese Han population.

Ontology evolution has become more and more important to domain ontology application. The requirements are the basis of ontology evolution. This paper gives a model for auto-generated requirements of domain ontology evolution from domain text. First it extracts relevant concepts from domain text in natural language, and then uses the weighted word frequency algorithm to determine whether the concepts are the key concepts. Last, combining the ontology-search-algorithm with the rules of generating evolution requirements, it automatically generates the requirements of domain ontology evolution.

A half-self-similar solution on the bases of the equations of the continuity and motion of ions and Poisson’s equation is proposed for isothermal expansions of nonquasineutral plasmas with an enhanced electron tail into a vacuum from solid targets heated by ultraintense laser pulses. The ion expansion is assumed to be self-similar, while the electron expansion is non-self-similar. The expression of the self-similar variable and maximum ion velocity at the ion front is given out. It is shown that the ion acceleration with an enhanced hot-electron tail is more efficient than that with Maxwellian electrons.

The self-similar solutions are obtained for isothermal expansions of neutral plasmas into a vacuum. The classic solution given by Mora [Phys. Fluids 22, 12 (1979)] corresponds to a special case of our solution. Some special solutions have been pointed out by Gurevich et al. [Phys. Rev. Lett. 42, 769 (1979)] and observed by a lot of experiments. The formulation of ion velocity with respect to the acceleration time is proposed for the general solution, and the numerical result can be obtained easily. The electric field and ion velocity at the ion front have been achieved.

A model, called the Step Model, is proposed to describe hot-electron recirculation. A formula to estimate electron density at the rear side sheath is described. With a fixed initial hot-electron density for some target thicknesses, the results from the Step Model are compared with several experiments. The influences of laser pulse absorption efficiency, laser pulse duration, the opening angle of hot-electrons, hot-electron recirculation, and target thickness on the ion acceleration are discussed. When the target thickness is far less than the laser focus radius, the initial electron density will be proportional to the laser pulse absorption efficiency, and the angular effect and the thickness effect can both be ignored. For any target thickness, the maximum ion velocity accelerated by the rear side sheath can be calculated using the Step Model. As an application to some experiments, the Step Model provides a half-analytic method to achieve the dependence of the laser pulse absorption efficiency on target thickness and the influence of amplified spontaneous emission (ASE) duration on the laser absorption efficiency.