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Teacher self-efficacy plays an important role in special education; yet, the literature on the measurement of teacher self-efficacy in this field is limited. This study investigated the psychometric properties of the Teachers’ Sense of Efficacy Scale with a sample of Chinese special education teachers ( N = 401, 24.19% male). Confirmatory factor analysis indicated that the Chinese special educational version of the Teacher's Sense of Efficacy Scale (CS-TSES), modified from the Asian model by Ruan, J., Nie, Y., Hong, J., Monobe, G., Zheng, G., Kambara, H., & You, S. (2015). Cross-cultural validation of teachers’ sense of efficacy scale in three Asian countries. Journal of Psychoeducational Assessment, 33(8), 769-779, showed an excellent fit. Additionally, correlations were observed between the results of the CS-TSES and both the Multidimensional Scale of Perceived Social Support and the Satisfaction with Life Scale, providing criterion-related validity. In sum, the findings indicated that the TSES is applicable to special education teachers in the Chinese context.

Genome-wide association studies indicate that SCN10A plays an important role in cardiac electrophysiology. Common and rare SCN10A variants are suggested to contribute to Brugada Syndrome (BrS), an inherited channelopathy resulting from genetic-determined loss-of-function in cardiac sodium channel. This study sought to characterize the role of SCN10A common variants in BrS. Clinical and genetic analyses were performed in 197 patients diagnosed with BrS. Baseline ECG parameters were evaluated in patients carrying each of four common variants associated with BrS. Cellular electrophysiological study was performed in SCN5A-SCN10A co-transfected TSA201 cells to investigate the possible electrophysiological characteristics of the allele of rs6795970, which displayed the most significant association with BrS. Four SCN10A common variants (rs7630989, rs57326399, rs6795970, rs12632942) displayed significant association with BrS susceptibility. There were no evident associations between baseline ECG parameters in BrS patients and the different genotypes of the four variants. Rs6795970 (V1073) was strongly associated with a risk for BrS, which suggests the different electrophysiological characters between these two alleles. Functional study showed a positive shift in steady-state activation (V1/2: −62.2 ± 2.6 vs. –53.5 ± 1.6 for A1073 and V1073 group, respectively; P

Four antibiotics[azithromycin (AZM), sulfamethoxazole (SMZ), ciprofloxacin (CIP), and tetracycline (TCY)], and the antibiotic resistance genes (ARGs)[sulfonamides (

To observe the efficacy of "Tongdu Tiaoshen" (dredging Governor Vessel and regula-ting mind,needling on the cognitive function of patients with sepsis associated encephalopathy (SAE).A total of 64 patients with SAE were enrolled in the present study, and randomly and equally divided into a control group and a treatment group. Patients in the control group received conventional medicines and conventional needling treatment. The patients of the treatment group received conventional medicines and "Tongdu Tiaoshen" needling treatment. The treatment was conducted once daily for 10 days. The Montreal Cognitive Assessment (MoCA) scale was used to assess the therapeutic effect after the treatment. Serum interleukin-6 (IL-6) was detected by radioimmunoassay, serum C-reactive protein (CRP) was detected by immuno-scattering method, and arterial blood lactic acid (Lac) content was detected by blood gas analyzer.The effective rate in the treatment group was obviously higher than that in the control group ("Tongdu Tiaoshen" needling can significantly improve the cognitive function of SAE patients, which may be associated with its effect in reducing inflammatory reaction of sepsis.

Background This study investigates the relationships and mechanism between perceived social support, loneliness, and life satisfaction among Chinese parents of children with autism spectrum disorder (ASD). Method Cross-sectional data were collected from 306 parents of children with ASD, including fathers and mothers, in Guangzhou, Guangdong province, mainland China. A survey comprising the parent-rated Strength and Difficulties Questionnaire, the Multidimensional Scale of Perceived Social Support, the UCLA Loneliness Scale, and the Satisfaction with Life Scale was administered. Results Perceived social support was significantly associated with loneliness and life satisfaction. Loneliness both mediated and moderated the relationship between perceived social support and life satisfaction. Conclusions Theoretically, the findings highlight the role of loneliness in explaining the mechanism through which perceived social support is linked to life satisfaction. Practically, this study suggests that high levels of perceived social support and low levels of loneliness are socio-psychological resources that can be targeted to enhance the life satisfaction of Chinese parents of children with ASD.

Cardiac hypertrophy is characterized by increased myofibrillogenesis. Angiotensin II (Ang-II) is an essential mediator of the pressure overload–induced cardiac hypertrophy in part through RhoA/ROCK (small GTPase/Rho-associated coiled-coil containing protein kinase) pathway. FHOD3 (formin homology 2 domain containing 3), a cardiac-restricted member of diaphanous-related formins, is crucial in regulating myofibrillogenesis in cardiomyocytes. FHOD3 maintains inactive through autoinhibition by an intramolecular interaction between its C- and N-terminal domains. Phosphorylation of the 3 highly conserved residues (1406S, 1412S, and 1416T) within the C terminus (CT) of FHOD3 by ROCK1 is sufficient for its activation. However, it is unclear whether ROCK-mediated FHOD3 activation plays a role in the pathogenesis of Ang-II–induced cardiac hypertrophy. In this study, we detected increases in FHOD3 expression and phosphorylation in cardiomyocytes from Ang-II–induced rat cardiac hypertrophy models. Valsartan attenuated such increases. In cultured neonate rat cardiomyocytes, overexpression of phosphor-mimetic mutant FHOD3-DDD, but not wild-type FHOD3, resulted in myofibrillogenesis and cardiomyocyte hypertrophy. Expression of a phosphor-resistant mutant FHOD3-AAA completely abolished myofibrillogenesis and attenuated Ang-II–induced cardiomyocyte hypertrophy. Pretreatment of neonate rat cardiomyocytes with ROCK inhibitor Y27632 reduced Ang-II–induced FHOD3 activation and upregulation, suggesting the involvement of ROCK activities. Silencing of ROCK2, but not ROCK1, in neonate rat cardiomyocytes, significantly lessened Ang-II–induced cardiomyocyte hypertrophy. ROCK2 can directly phosphorylate FHOD3 at both 1412S and 1416T in vitro and is more potent than ROCK1. Both kinases failed to phosphorylate 1406S. Coexpression of FHOD3 with constitutively active ROCK2 induced more stress fiber formation than that with constitutively active ROCK1. Collectively, our results demonstrated the importance of ROCK2 regulated FHOD3 expression and activation in Ang-II–induced myofibrillogenesis, thus provided a novel mechanism for the pathogenesis of Ang-II–induced cardiac hypertrophy.

Cardiac fibrosis (CF), a repairing process following myocardial infarction (MI), is characterized by abnormal proliferation of cardiac fibroblasts and excessive deposition of extracellular matrix (ECM) resulting in inevitable resultant heart failure. TGF-β (transforming growth factor-β)/ALK5 (Activin receptor-like kinase 5)/Smad2/3/4 pathways have been reported to be involved in the process. Recent studies have implicated both activin and its specific downstream component ALK4 in stimulating fibrosis in non-cardiac organs. We recently reported that ALK4 is upregulated in the pressure-overloaded heart and its partial inhibition attenuated the pressure overload-induced CF and cardiac dysfunction. However, the role of ALK4 in the pathogenesis of MI-induced CF, which is usually more severe than that induced by pressure-overload, remains unknown. Here we report: 1) In a wild-type mouse model of MI, ALK4 upregulation was restricted in the fibroblasts of the infarct border zone; 2) In contrast, ALK4+/- mice with a haplodeficiency of ALK4 gene, showed a significantly attenuated CF in the border zone, with a smaller scar size, a preserved cardiac function and an improved survival rate post-MI; 3) Similarly to pressure-overloaded heart, these beneficial effects might be through a partial inactivation of the Smad3/4 pathway but not MAPK cascades; 4) The apoptotic rate of the cardiomyocytes were indistinguishable in the border zone of the wild-type control and ALK4+/- mice; 5) Cardiac fibroblasts isolated from ALK4+/- mice showed reduced migration, proliferation and ECM synthesis in response to hypoxia. These results indicate that partial inhibition of ALK4 may reduce MI-induced CF, suggesting ALK4 as a novel target for inhibition of unfavorable CF and for preservation of LV systolic function induced by not only pressure-overload but also MI.

Previous studies have demonstrated that the benzo[c]phenanthridine alkaloid chelerythrine chloride (CC) has inhibitory effects on various tumors. However, the anticancer activity of CC and its underlying mechanisms have not been elucidated in renal cancer cells. The present study examined the effects of CC on growth inhibition and apoptosis of renal cancer cells in vitro and in vivo. Flow cytometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays revealed that CC markedly suppressed the growth of HEK-293 and human renal cancer SW-839 cells in a time- and dose-dependent manner. The xenograft mouse model, which was performed in nude mice, exhibited a reduced tumor growth following CC treatment. In addition, the present study revealed that CC significantly decreased the phosphorylation of extracellular signal-regulated kinase (ERK) and Akt, which was accompanied by upregulation of p53, B-cell lymphoma 2 (Bcl-2)-associated X protein, cleaved caspase-3 and cleaved poly (adenosine diphosphate-ribose) polymerase (PARP), and downregulation of Bcl-2, caspase-3 and PARP. Furthermore, the use of PD98059, a specific mitogen-activated protein kinase kinase inhibitor, potentiated the proapoptotic effects of CC, which indicated that CC may induce apoptosis in renal cancer cells partly via inhibition of ERK activity. Overall, the results of the present study demonstrated that CC may be developed as a potential anticancer treatment for patients with renal cancer.

Background This study investigated primary school teachers’ knowledge of autism spectrum disorder (ASD) and their attitude and professional self-efficacy regarding children with ASD. Method Four hundred and ten mainstream primary school teachers were assessed using the Autism Stigma and Knowledge Questionnaire, the Autism Attitudes Scale for Teachers and a professional self-efficacy scale. Results The results showed that the teachers’ knowledge of ASD was significantly correlated with their attitude and professional self-efficacy regarding children with ASD. Attitude both mediated and moderated the correlation between knowledge and professional self-efficacy. Conclusions The study demonstrated that knowledge and attitude are important indicators of professional self-efficacy. As such, teachers’ professional self-efficacy regarding children with ASD can be improved by improving their knowledge of ASD and attitude toward these children.

Patients with high-risk long QT syndrome (LQTS) mutations may experience life-threatening cardiac events. The present study sought to characterize a novel pathogenic mutation, KCNQ1p.Thr312del, in a Chinese LQT1 family. Clinical and genetic analyses were performed to identify this novel causative gene mutation in this LQTS family. Autosomal dominant inheritance of KCNQ1p.T312del was demonstrated in the three-generation pedigree. All mutation carriers presented with prolonged QT intervals and experienced recurrent syncope during exercise or emotional stress. The functional consequences of the mutant channel were investigated by computer homology modeling as well as whole-cell patch-clamp, western-blot and co-immunoprecipitation techniques using transfected mammalian cells. T312 is in the selectivity filter (SF) of the pore region of the KCNQ1-encoded channel. Homology modeling suggested that secondary structure was altered in the mutant SF compared with the wild-type (WT) SF. There were no significant differences in Kv7.1 expression, membrane trafficking or physical interactions with KCNE1-encoded subunits between the WT and mutant transfected channels. However, the KCNQ1p.T312del channels expressed in transfected cells were non-functional in the absence or presence of auxiliary KCNE1-subunits. Dominant-negative suppression of current density and decelerated activation kinetics were observed in cells expressing KCNQ1WT and KCNQ1p.T312del combined with KCNE1 (KCNQ1WT/p.T312del + KCNE1 channels). Those electrophysiological characteristics underlie the pathogenesis of this novel mutation and also suggest a high risk of cardiac events in patients carrying KCNQ1p.T312del. Although protein kinase A-dependent current increase was preserved, a significant suppression of rate-dependent current facilitation was noted in the KCNQ1WT/p.T312del + KCNE1 channels compared to the WT channels during 1- and 2-Hz stimulation, which was consistent with the patients’ phenotype being triggered by exercise. Overall, KCNQ1p.Thr312del induces a loss of function in channel electrophysiology, and it is a high-risk mutation responsible for LQT1.

Background: Phospholemman (PLM) is an important phosphorylation substrate for protein kinases A and C in the heart. Until now, the association between PLM phosphorylation status and L-type calcium channels (LTCCs) gating has not been fully understood. We investigated the kinetics of LTCCs in HEK 293T cells expressing phosphomimetic or nonphosphorylatable PLM mutants. Methods: The LTCCs gating was measured in HEK 293T cells transfected with LTCC and wild-type (WT) PLM, phosphomimetic or nonphosphorylatable PLM mutants: 6263AA, 6869AA, AAAA, 6263DD, 6869DD or DDDD. Results: WT PLM significantly slowed LTCCs activation and deactivation while enhanced voltage-dependent inactivation (VDI). PLM mutants 6869DD and DDDD significantly increased the peak of the currents. 6263DD accelerated channel activation, while 6263AA slowed it more than WT PLM. 6869DD significantly enhanced PLM-induced increase of VDI. AAAA slowed the channel activation more than 6263AA, and DDDD accelerated the channel VDI more than 6869DD. Conclusions: Our results demonstrate that phosphomimetic PLM could stimulate LTCCs and alter their dynamics, while PLM nonphosphorylatable mutant produced the opposite effects.

A method on green plants recognition based on wavelet transform and varied local edge patterns (VLEP) is proposed in this paper. Firstly the original image is decomposed by wavelet transformation. Then texture features are extracted using VLEPs. At the same time, block-based and multi-resolution ideas are considered together to extract features after images are transformed by wavelet. Finally, the fused texture features are classified by the nearest neighbor method. The experimental results show that the proposed method is a promising method for recognizing the green plants. And combination of block-based and multi-resolution ideas can further improve the accuracy effectively.

The worse coal quality cause low center flame temperature in furnace, low combustion stability, high carbon content in fly ash and slag. The refractory belt installed in primary combustion zone can reduce the heat absorption capacity of water wall and improve the average temperature to steady ignition and high efficient combustion. For conforming the area of refractory belt better, the equation was founded by thermal computational methods and analyzing the different influence to the area of refractory belt.

E-Box multimedia system is developed for the rich audio and video resource on the Internet and on its server side, it can automatically search and integration of network video and audio resources, and send to the client side for the user in real-time broadcast TV viewing, full use of remote control operation, Simply its a very easy to use multimedia system. This article introduces its infrastructure, main technical ideas and you can also see some details about server side and client side.

E-Yuan multimedia system is developed for the rich audio and video resource on the Internet and on its server side, it can automatically search and integration of network video and audio resources, and send to the client side for the user in real-time broadcast TV viewing, full use of remote control operation, Simply it’s a very easy to use multimedia system. This article introduces its infrastructure, main technical ideas and you can also see some details about server side and client side.

The objective of this work was to use a novel 3-D test analysis system for evaluating the mechanical properties of the natural fiber fabric composites under biaxial loads. Composites with three resin matrices (water-based epoxy resin, isocyanate resin, phenolic resin) were investigated, and strain filed were characterized by using the digital speckle correlation. The water-based epoxy resin plate and isocyanate resin plate demonstrated a characteristic of orthotropy and elastoplasticity, while the phenolic resin board revealed linear elastic and brittle-fracture simultaneously at X,Y-axial. Dissimilarities of biaxial load value were related to the orthotropy of composite structure, and load changes in fracture direction had a negative effect on the other directions at breaking moment. The degrees of dropping presented a positive correlation with the load values at rift direction. Under the linear elastic stage, the value of load and average strain at Y-direction were larger than that under X-direction within the same testing area. The strain-filed at X/Y-direction provided by isocyanate and phenolic resin plates illustrated a more smooth change than that of the water-based epoxy resin plate.

To evaluate the mechanical properties of natural fiber fabrics with the characteristic of interweaving between warp and weft direction as well as textile structural composites under complex stress state, it is essential to develop experimental techniques to realize multi-axial testing for large deformation. This work proposes a novel testing device for investigating fiber fabrics and textile composites under multidimensional loads. Accuracy of the system is comparable to the testing diagnostics of the Instron 5808 previously. The homogeneity for different types of cruciforms and different biaxial tensile speeds were investigated. The effects of different loading methods, biaxial (X and Y), and 3-D loading (X, Y, and Z) on the breaking strength of the woven ramie fabric were presented. The displacement-field and shear field for composites with hole were characterized by the digital speckle correlation method.

A novel ZnO rod-needle nanostructure with a gold nanoparticle on the tip has been prepared by combining electrochemical deposition and thermal evaporation methods. The morphologies were characterized by field-emission scanning electron microscopy and transmission electron microscopy. It is found that only a single ZnO nanoneedle is located on the top of each ZnO nanorod. The density and length of this ZnO rod-needle nanostructure can be tuned feasibly. An intriguing phenomenon of crystal lattice rotation between ZnO nanoneedle and nanorod segments is observed and explained in detail. The adoption of ZnS as the source plays an important role in the formation of this structure and the rotation of crystal planes.

In this paper, ZnGa2O4 hierarchical nanostructures with comb-like morphology are fabricated by a simple two-step chemical vapor deposition (CVD) method: first, the Ga2O3 nanowires were synthesized and employed as templates for the growth of ZnGa2O4 nanocombs; then, the as-prepared Ga2O3 nanowires were reacted with ZnO vapor to form ZnGa2O4 nanocombs. Before the reaction, the Au nanoparticles were deposited on the surfaces of Ga2O3 nanowires and used as catalysts to control the teeth growth of ZnGa2O4 nanocombs. The as-prepared ZnGa2O4 nanocombs were highly crystallized with cubic spinel structure. From the photoluminescence (PL) spectrum, a broad band emission in the visible light region was observed of as-prepared ZnGa2O4 nanocombs, which make it promising application as an optical material.

In this paper, submicrometer-sized Ga–In eutectic alloy particles were dispersed into polymethylmethacrylate (PMMA) matrix by ultrasonic vibration and sedimentation method. The solidification and melting processes of Ga–In eutectic alloy particles were studied by differential scanning calorimeter (DSC). Four endothermal peaks with the onset temperature located at 16, −11, −22, and −27 °C were observed in DSC heating curves, which corresponded to the melting process of the stable Ga–In phase α-Ga(In) and three metastable phases of β-Ga(In), δ-Ga(In) and γ-Ga(In), respectively. The stable phase α-Ga(In) can only be formed when the size of alloy particle was larger than 0.58 μm. Conversely, metastable phases β-Ga(In), δ-Ga(In) and γ-Ga(In) are mainly formed. The result shows that phase structures in Ga–In eutectic alloy are size dependent.

Chinese medicinal formulae (CMF) were usually used in the clinics of traditional Chinese medicines (TCM), which were critical for modernization of Chinese medicine to shed light on the interaction between CMF and biological organisms. However, correlation between system and part, macroscopic actions and microcosmic mechanism, ADME process and pharmacologic actions were usually neglected. The put-forward of integrative pharmacology provided a feasible approach to solve the problem of the fragmentation of TCM. For the past years, we applied the strategy of integrative pharmacology to study Yuanhu Zhitong prescription( YZP) systematically, and established two modes, chemical fingerprints-metabolism fingerprints-network targets and intestinal absorption-activity evaluation-data mining, to establish the interaction rule between the chemical composition and biological activity from multiple levels, such as the calculation and in vitro/vivo, which provided proof for the quality control, pharmacodynamic material basis and pharmacological action of YZP. In this paper, we summarized the related progresses of the research of YZP.

Background Previous studies have demonstrated that WenXin KeLi (WXKL), a traditional Chinese medicine, can exert antiarrhythmic properties through complex multichannel inhibition, but its pharmacologic effect remains to be elucidated, especially in the cardiac conductive system. Objective To explore the antiarrhythmic property of WXKL in cardiac Purkinje cells (PCs). Methods PCs were isolated from rabbit hearts and action potentials (APs) and ion currents were recorded by whole-cell patch clamp technique. Anemonia toxin II (ATX-II) and isoproterenol (ISO) were used to induce early or delayed afterdepolarizations (EADs, DADs) or triggered activities (TAs). Results WXKL (1 g/L and 5 g/L) significantly abbreviated the action potential duration (APD) of PCs in a dose- and rate-dependent manner. Treatment of PCs with ATX-II (2 nM) prolonged APD and induced EADs, which were significantly suppressed by WXKL. WXKL (1, 5 g/L) also inhibited ISO-induced EADs, DADs, and TAs. To reveal the ionic mechanisms, we studied the effects of WXKL on late sodium current (I NaL ), peak sodium current (I NaP ), and L-type calcium currents (I CaL ) in PCs. WXKL-attenuated ATX-II (5 nM) induced I NaL augmentation and blocked I NaL with an IC 50 of 4.3 ± 0.5 g/L, which is 3- to 4-fold more selective than that of I NaP (13.3 ± 0.9 g/L) and I CaL (17.6 ± 1.4 g/L). Moreover, WXKL exerted significantly less use-dependent block of I NaP than that of flecainide, indicating its lower proarrhythmic effect. Conclusions WXKL exhibits antiarrhythmic properties in cardiac PCs via selective inhibition of I NaL .

Nanometre-sized (hereafter n-) Pb particles embedded in an Al matrix were prepared by ball milling, and the surface melting behaviour was studied by the internal friction technique using a dynamic mechanical analyser. There is an internal friction peak appearing around Pb melting point and the height of the internal friction peak decreases with the increase of Pb particle size. Surface melting of n-Pb particles accounts for the internal friction peak. The study may throw light on the nature of surface melting in condensed matter physics. In addition, the present work makes a beneficial attempt at exploring internal friction as an experimental method for studying surface melting.

Anodic aluminum oxide (AAO) templates with highly ordered nanoporous structure were fabricated by means of the electrochemical anodization under the constant anodic voltage and electrolyte temperature. The dependence of the ordering degree of nanopores on the point defects, dislocation configuration and grain boundary of aluminum is qualitatively analyzed. Experiment results show that the size of the ordered region of nanopores depends strongly on the point defects, dislocation cell configuration.

Cardiac engineering of patches and tissues is a promising option to restore infarcted hearts, by seeding cardiac cells onto scaffolds and nurturing their growth in vitro. However, current patches fail to fully imitate the hierarchically aligned structure in the natural myocardium, the fast electrotonic propagation, and the subsequent synchronized contractions. Here, superaligned carbon-nanotube sheets (SA-CNTs) are explored to culture cardiomyocytes, mimicking the aligned structure and electrical-impulse transmission behavior of the natural myocardium. The SA-CNTs not only induce an elongated and aligned cell morphology of cultured cardiomyocytes, but also provide efficient extracellular signal-transmission pathways required for regular and synchronous cell contractions. Furthermore, the SA-CNTs can reduce the beat-to-beat and cell-to-cell dispersion in repolarization of cultured cells, which is essential for a normal beating rhythm, and potentially reduce the occurrence of arrhythmias. Finally, SA-CNT-based flexible one-piece electrodes demonstrate a multipoint pacing function. These combined high properties make SA-CNTs promising in applications in cardiac resynchronization therapy in patients with heart failure and following myocardial infarctions.

Activin receptor-like kinase 4 (ALK4), a downstream receptor of transforming growth factor-β superfamily, is highly expressed in the mammal heart. We recently reported that ALK4 is upregulated in pressure-overloaded heart and ALK4 haplodeficiency attenuated the pressure overload-induced cardiac

Background: Hydrogen peroxide (H2O2)-induced oxidative stress has been demonstrated to induce afterdepolarizations and triggered activities in isolated myocytes, but the underlying mechanisms remain not fully understood. We aimed to explore whether protein kinase C (PKC) activation plays an important role in oxidative stress-induced afterdepolarizations. Methods: Action potentials and ion currents of isolated rabbit cardiomyocytes were recorded using the patch clamp technique. H2O2 (1 mM) was perfused to induce oxidative stress and the specific classical PKC inhibitor, Gö 6983 (1 μM), was applied to test the involvement of PKC. Results: H2O2 perfusion prolonged the action potential duration and induced afterdepolarizations. Pretreatment with Gö 6983 prevented the emergence of H2O2-induced afterdepolarizations. Additional application of Gö 6983 with H2O2 effectively suppressed H2O2-induced afterdepolarizations. H2O2 increased the late sodium current (INa,L) (n = 7, p < 0.01) and the L-type calcium current (ICa,L) (n = 5, p < 0.01), which were significantly reversed by Gö 6983 (p < 0.01). H2O2 also increased the transient outward potassium current (Ito) (n = 6, p < 0.05). However, Gö 6983 showed little effect on H2O2-induced enhancement of Ito. Conclusions: H2O2 induced afterdepolarizations via the activation of PKC and the enhancement of ICa,L and INa,L. These results provide evidence of a link between oxidative stress, PKC activation and afterdepolarizations.

Nowadays, detecting fetal ECG using abdominal signal is a commonly used method, but fetal ECG signal will be affected by maternal ECG. Current FECG extraction algorithms are mainly aiming at multiple channels signal. They often assume there is only one fetus and did not consider multiple births. This paper proposed a single channel blind source separation (SCBSS) algorithm based on source number estimation using multi-algorithm fusion to process single abdominal signal. The method decomposed collected single channel signal into multiple intrinsic mode function (IMF) utilizing Empirical Mode Decomposition (EMD), mapping single channel into multiple channels. Four multiple channel source number estimation (MCSNE) methods (Bootstrap, Hough, AIC and PCA) were weighting fused to estimate accurate source number and the particle swarm optimization algorithm (PSO) was employed to determine weighted coefficient. According to source number and IMF, nonnegative matrix was constructed and nonnegative matrix factorization (NMF) was employed to separate mixed signals. Experiments used single channel signal mixed by four man-made signals and single channel ECG mixed by two to verify the proposed algorithm. Results showed that the proposed algorithm could determine number of independent signal in single acquired signal. FECG could be extracted from single channel observed signal and the algorithm can be used to solve separation of MECG and FECG.

Aims Myocardial infarction (MI) induces neural remodelling of the left stellate ganglion (LSG), which may contribute to ischaemia-induced arrhythmias. The neural chemorepellent Semaphorin 3a (Sema3a) has been identified as a negative regulator of sympathetic innervation in the LSG and heart. We previously reported that overexpression of Sema3a in the border zone could reduce the arrhythmogenic effects of cardiac sympathetic hyperinnervation post-MI. This study investigated whether Sema3a overexpression within the LSG confers an antiarrhythmic effect after MI through decreasing extra- and intra-cardiac neural remodelling. Methods and results Sprague-Dawley rats were subjected to MI, and randomly allocated to intra-LSG microinjection of either phosphate-buffered saline (PBS), adenovirus encoding green fluorescent protein (AdGFP), or adenovirus encoding Sema3a (AdSema3a). Sham-operated rats served as controls. Two weeks after infarction, MI-induced nerve sprouting and sympathetic hyperinnervation in the LSG and myocardium were significantly attenuated by intra-LSG injection with AdSema3a, as assessed by immunohistochemistry and western blot analysis of growth-associated protein 43 and tyrosine hydroxylase. This was also confirmed by sympathetic nerve function changes assessed by cardiac norepinephrine content. Additionally, intra-LSG injection with AdSema3a alleviated MI-induced accumulation of dephosphorylated connexin 43 in the infarct border zone. Furthermore, Sema3a overexpression in the LSG reduced the incidence of inducible ventricular tachyarrhythmia by programmed electrical stimulation post-MI, and arrhythmia scores were significantly lower in the AdSema3a group than in the PBS and AdGFP groups. Conclusion Semaphorin 3a overexpression in the LSG ameliorates the inducibility of ventricular arrhythmias after MI, mainly through attenuation of neural remodelling within the cardiac-neuraxis.

Submicrometer-sized Ga particles were dispersed in polymethylmethacrylate (PMMA) matrix by an ultrasonic vibration and sedimentation method. The solid phase transition from γ-Ga to δ-Ga and its Ga particle size dependence were studied by means of differential scanning calorimeter measurements. It was shown that a solid-solid phase transition corresponding to the [Formula: see text]-Ga one happened in Ga particles upon cooling. Moreover, the ratio of the particles undergoing the solid phase transition to all particles increases with decrease of the particle size.

The size effect of materials on the phase structure has been proved by many experiments, and here for the first time, we proposed a size-temperature phase diagram of gallium (Ga). We found that liquid Ga as well as its solid-liquid phase transition (PT) is size dependent. This PT phenomenon was successfully explained in terms of nucleation theory. The size-temperature phase diagram offered a universal phenomenological explanation for the size-related phase selection of Ga. This finding would enrich the general knowledge about the new phenomena induced by the finite-size effect.