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Bone marrow transplantation is regarded as the most effective immunotherapy for hematologic cancer, but it generally faces difficulties in matching. Aberrant expression of histone deacetylases (HDACs) is closely related to the occurrence and development of hematological cancer. Recent studies suggested that HDACs might play a critical role in initiating anti-cancer immune response or enhancing anti-cancer immunotherapy. Besides, combining HDAC inhibition and immunotherapy could prevent immunotherapy resistance in some degree and reach an extended treatment window. This review summarized the relationship between HDACs and immune and described the current understanding of HDACs in immunotherapy for hematologic cancer.

Autophagy is the process by which intracellular components are delivered to lysosomes or vacuoles for degradation and recycling, which can promote the tolerance of organisms to biotic/abiotic stresses. However, autophagy-related genes (ATG) are not well studied in woody plants. Here, 48 ATG genes were identified in the poplar genome and divided into 14 subfamilies according to the phylogenetic tree. Collinearity analysis showed that 26 pairs of genes were derived by segmental duplication in poplars. The isogenous gene pairs of the ATG family between P. trichocarpa and other six species were analyzed by synteny analysis. Moreover, the ATG promoters contain a large number of phytohormone response elements and stress-response elements. Both phytohormone and salt treatments can induce the expression of PagATG18 subfamily genes. Overexpression of PagATG18a significantly improved the salt tolerance of poplar and reducing the oxidative damage of the membrane. Further research verified that PagATG18a interacted with the light-harvesting complex LHCB1 and APX2, indicating PagATG18a might be involved in regulating photosynthesis and antioxidant activity under stress. This study provides valuable information for further research on the functional characteristics of ATG genes in poplar and the theoretical basis for poplar stress resistance breeding.

An-Hai Li,1 Wen-Wen Li,2 Xiao-Qian Yu,3 Dai-Ming Zhang,4 Yi-Ran Liu,5 Ding Li1 1Department of Dermatology, Qingdao Huangdao District Central Hospital, Qingdao, People’s Republic of China; 2Department of Hematology, Qingdao Women and Children’s Hospital, Qingdao, People’s Republic of China; 3Department of Dermatology, Qingdao Haici Hospital (Qingdao Traditional Chinese Medicine Hospital), Qingdao, People’s Republic of China; 4Department of Pharmacy, Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 5College of Traditional Chinese Medicine, Weifang Medical College, Weifang, People’s Republic of ChinaCorrespondence: Ding Li, Department of Dermatology, Qingdao Huangdao District Central Hospital, Qingdao, People’s Republic of China, Email 907325791@qq.comBackground: Psoriasis is a recurrent, chronic, inflammation- and immune-mediated skin disease with multiple causative factors. However, the genetic markers associated with recurrence have not yet been fully elucidated. Accordingly, in this study, we aimed to identify markers associated with the recurrence of psoriasis.Methods: We analyzed differentially expressed genes to determine which targets were associated with the recurrence of psoriasis and used these data to construct a protein-protein interaction network using Cytoscape software. The results were then validated by analysis of core targets using Gene Expression Omnibus (GEO) datasets and clinical samples. Functional enrichment analysis was used to explore the potential mechanisms mediating the recurrence of psoriasis.Results: We screened out six core targets that played important roles in recurrence of psoriasis, and validation of GEO datasets and clinical samples showed that the expression levels of five core targets were higher in patients with psoriasis than in healthy individuals. Functional enrichment analysis revealed that the cell cycle and oocyte meiosis signaling pathways were involved in the recurrence of psoriasis.Conclusion: Our findings provided insights into the mechanisms mediating the onset and recurrence of psoriasis.Keywords: psoriasis, recurrence, cell cycle, gene expression profiling, pathogenic mechanism

Psoriasis is an independent risk factor for coronary heart disease. It is important for predicting the complications of coronary heart disease in patients with psoriasis.In this study, related cases were collected from the case system of Qingdao University, and commonly used laboratory indicators were extracted. Artificial neural network (ANN) and logistics regression analysis were used to learn to distinguish psoriasis patients, coronary heart disease patients, and psoriasis patients with coronary heart disease. We identified independent risk factors for coronary heart disease in psoriasis patients that exacerbate coronary heart disease symptoms in patients with psoriasis.Analysis shows that the accuracy of the ANN model was higher than 79%. It was determined that age, chlorinated, phosphorus, magnesium, low-density lipoprotein, triglycerides, high density lipoprotein and total cholesterol are independent risk factors for coronary heart disease in patients with psoriasis. Similarly, gender, age, chlorinated, magnesium, triglycerides, and high density lipoprotein are risk factors that exacerbate coronary heart disease symptoms in patients with psoriasis.The presented approach is a valuable tool for identifying psoriasis patients, coronary heart disease patients, and psoriasis patients with coronary heart disease. It can also serve as a support tool clinicians in the diagnostic process, by providing an outstanding support in the diagnostics prevention of coronary heart disease in psoriasis.

In this work, a novel label-free chemiluminescent (CL) assay system was developed for highly sensitive and selective detection of adenosine triphosphate (ATP). The strategy relies on the powerful signal amplification capability of rolling circle amplification (RCA), the preferential binding ability of graphene oxide (GO) to single-stranded DNA (ssDNA), and instantaneous derivatization reaction between phenylglyoxal (PGO) and guanine nucleobases (G). In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction between the two ends of the padlock probe producing a closed circular DNA template that initiates the RCA reaction with phi29 DNA polymerase and dNTP. Therein, many complementary copies of the circular template can be generated. The formed long single-stranded DNA which contained an amount of guanine bases could be adsorbed on the surface of GO forming DNA-GO complexes. And the CL signal of DNA-GO complexes can be obtained via the instantaneous derivatization reaction between PGO and guanine bases. The CL signal increased linearly with the concentration of ATP from 0.1 to 2.5 nM with a detection limit of 0.03 nM. The system also showed high specificity to ATP against its analogues such as CTP, GTP, UTP, AMP, ADP, BSA and CaM. In addition, ATP has been determined in diluted serum indicating the applicability of this assay.

The antibacterial properties of silver nanoparticles may be able to prevent inflammation around an implant when placed on the surface of the dental neck. The purpose of this study was to culture silver nanoparticles on human bone marrow stem cells in vitro to determine their effects on cell differentiation and to assess their biocompatibility. Silver nanoparticles were deposited on titanium foil implant surfaces using ion sputtering, and adult bone marrow mesenchymal stem cells were cultured on those surfaces to determine the effect of the silver nanoparticles on cell viability, suitability for subculture, proliferation and differentiation. The results indicated that the silver nanoparticles were biocompatible, allowing cell proliferation and differentiation of osteoblasts, but interfered with differentiation into chondrocytes and adipocytes.

Objective: To explore the effect of nanosilver on inducement of differentiation of neural stem cells of SD rats. Method: nanosilver film and newborn SD rat neural stem cell co-culture method is adopted to perform primary and subculture on SD rat neural stem cells and to induce differentiation. Nestin staining is adopted to identify the characteristics of neural stem cells. Immunohistochemistry method (β-III-tubulin, GFAP staining) is adopted to detect the status of differentiation from neural stem cells into neurons and neurogliocytes. Result: The neural stem cells of newborn SD rats can form Nestin positive cell balls in case of no serum culture. After induction of differentiation, NF-positive cells and GFAP-positive cells can be seen through immunofluorescence staining, wherein for nanosilver intervention group, the proportion of hippocampal NSCs differentiating to group neurons is smaller than that of the control group, with significantly reduced impact on astrocyte differentiation. Conclusion: nanosilver has adverse effect on normal differentiation of hippocampal NSCs to neurons and astrocytes.

Bromophenol red, known as chemical indicator, was found to act as a novel potent signal enhancer of the peroxidase-catalyzed luminol-H2O2 chemiluminescent (CL) reaction. It was found interestingly that bovine serum albumin (BSA) played a role in the enhanced chemiluminescent reaction (ECR). The addition of 2.5 mg mL(-1) BSA into bromophenol red-enhance CL system showed 36 times stronger CL signal than that without addition of BSA. Mechanism study showed that the luminophors in the ECR were still 3-aminophthalate ion in an excited state (3-APA*). In addition, singlet oxygen ((1)O2) and hydroxyl radical ((∙)OH) played a role in the ECR. The possible mechanism was discussed in the present study. The effect of pH, reaction time, and concentration of bromophenol red, BSA, luminol, and H2O2 on CL intensity of the peroxidase-catalyzed CL reaction was studied. The detection limit value (LOD) of HRP and streptavidin-modified HRP in the proposed ECR with bromophenol red and BSA was 0.20 ng mL(-1) and 0.05 ng mL(-1), respectively. This novel luminol-H2O2-HRP-bromophenol red-BSA CL system was applied to the CL detection of sequence-specific DNA based on a magnetic separation process. As low as 0.4 fmol of target DNA could be sensitively detected using the proposed CL system without any amplification process. The obtained results demonstrate very promising perspectives for using bromophenol red and BSA to improve the sensitivity of CL detection of sequence-specific DNA. In addition, this novel ECR system can also be generalized for CL immunoassay, CL western blotting, and so on.

In this study, a novel label-free chemiluminescent (CL) sensing platform for trypsin and trypsin inhibitor screening was developed by taking advantage of a luminol–NaIO4-(cysteine (Cys)-containing peptide) system and graphene oxide (GO). The assay was based on the different catalytic activities of a Cys-containing peptide and a Cys-containing peptide–GO complex in a luminol–NaIO4 CL reaction. A strong CL emission was obtained when the Cys-containing peptide was mixed with NaIO4 and basic luminol, while a much lower CL signal was observed when the Cys-containing peptide–GO complex was injected into a mixture of NaIO4 and basic luminol instead of the Cys-containing peptide. Based on a room-temperature electron spin-resonance spectroscopy (ESR) study, we speculated that the catalytic activity of Cys-containing peptide in the luminol–NaIO4 CL system may be inhibited when the peptides were adsorbed on the GO surface. Based on the above observation, a label-free CL sensing platform for trypsin was developed. In the absence of trypsin, the Cys-containing peptide was adsorbed on the GO surface via electrostatic interactions resulting in a low CL signal. In contrast, in the presence of trypsin, the peptide can be catalytically hydrolyzed at the C-terminus of arginine (Arg), resulting in the release of Cys-containing residues from the surface of GO and subsequent CL recovery with the addition of luminol and NaIO4. The proposed method enabled the determination of trypsin with a detection limit of 7.3 pM, and can also be employed for screening of trypsin inhibitors. The method can be easily generalized for monitoring the hydrolysis activity of other proteases by simply changing the peptide substrate sequence.

An ultrasensitive and selective chemiluminescence (CL) assay system for the detection of platelet-derived growth factor (PDGF)-BB was developed by taking advantage of the powerful signal amplification capability of rolling circle amplification (RCA) and a hydroxylamine-amplified gold nanoparticle (Au NP)-based CL technique. Rabbit anti-human PDGF-BB polyclonal antibody was covalently coupled on the surface of a 96-well plate that offers the reactive N-oxysuccinimide (NOS) group. In the presence of PDGF-BB, the aptamer–primer oligonucleotides were bound to the surface-captured antigen, resulting in an analyte-specific single-stranded DNA that could serve as the prime for the RCA reaction. The captured aptamer–primer was extended through the RCA reaction forming a single-stranded tandem repeated biotinylated copy of the circular template. After binding of the RCA product with streptavidin-gold (SA-Au NPs), the assembled Au NPs were enlarged by a NH2OH–HAuCl4 redox reaction allowing gold metal to be catalytically deposited onto the surface of the Au NPs. After an oxidative gold metal dissolution, a huge number of Au3+ ions were released from the enlarged Au NPs and were determined by a simple and sensitive luminol CL reaction. With two successive amplification steps, the proposed CL assay system exhibits not only high sensitivity with the detection limit of PDGF-BB as low as 0.06 pM (corresponding to 3 amol in 50 μL of sample solution), but also high specificity by taking advantage of using aptamers as recognition elements. In addition, PDGF-BB has been determined in diluted serum indicating the applicability of this assay.

According to the multivariable coupling、 large time delay, non-linearity and time-varying and other difficulties of circulating fluidized bed boiler combustion system, a kind of control technology based on neural network to circulating fluidized bed boiler combustion system was presented. Actual parameter data of a paper mill in Kunming and neural network control principle were used in the establishment of a circulating fluidized bed boiler combustion system mathematical model and modified BP neural network algorithm training. Results of MATLAB simulation show that boiler combustion system control precision was effectively improved and good effects in production and application were got.

A high-efficiency Agrobacterium tumefaciens mediated transformation system was established through testing and optimising a range of variables using soybean cotyledonary as explants and glufosinate as selective agent. Seven cultivars (Heihe 19, Heihe 25, Heinong 37, Ha 03-3, YC-1, YC-2, and Zhonghuang 39) were used to screen the optimal cultivars. As a result, cultivars YC-1 and YC-2 were screened, which had the highest transient GUS expression percentage and shoot induction frequency. The cell density of Agrobacterium was found to be related with the transient GUS expression percentage; its optimal cell density was OD 0.8–1.0. It was beneficial to A. tumefaciens infection at 23 °C and dim light co-cultivation condition. Interestingly, the infection time showed no relation to the GUS transient percentage. Three types of antioxidant solutions were tested on their effects of alleviating cotyledonary explant browning and necrosis. The results showed that addition of 20 μM lipoic acid on solid CCM had the lowest cotyledonary explant necrosis and a high transient GUS expression rate. Following the optimized A. tumefaciens mediated transformation system, the bar gene was successfully transferred into soybean with the transformation efficiency reaching 14.71 %, more than 50 times increase from the un-optimized procedure. Southern and T 1 progeny analysis showed the exogenous gene had been integrated into the soybean genome and could be inherited next progeny.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is the most important and widely used technology which is mainly used to analyze the protein molecular weight. Fibrin zymography based on the SDS-PAGE is the best method for qualitative analysis of unknown plasminogen activators (PAs), especially for the analysis of molecular weight. In electrophoresis technique, molecular weight marker is the most important factor. However, it is difficult to detect protein molecular weight markers in fibrin zymography. In this study, some important factors, such as concentrations of fibrinogen and plasminogen, are discussed. Our results provide an efficient and convenient method which can clearly exhibit the dark blue bands of protein molecular weight (MW) markers and the transparent bands of PAs against the light blue background on one gel at the same time, and show high sensitivity.

A sensitive electrochemical sensor for the detection of parathion based on molecularly imprinted polymer of organic-inorganic hybrid sol-gel from PTMOS and chitosan onto glass electrode was constructed. The sensor exhibited good selectivity and sensitivity to parahtion．The procedure of forming recognition cavity and sensitivity to parathion on the imprinted electrode were demonstrated. A highly linear response to parathion in the concentration range of 3×10-7～1×10-4 mol/L was observed, with a detection limit of 1×10-7 mol/L (S/N=3). ParathSuperscript textion imprinted and nonimprinted polymer films were exposed to a series of closely related compounds and the sensor exhibited good selectivity and sensitivity to parathion.

To compare the special series of ultrasonic inserts with Gracey curettes in the effectiveness and efficiency for non-surgical periodontal treatment.A total of 30 patients with moderate to advanced chronic periodontal disease were treated with both ultrasonic inserts (ultrasonic group) and Gracey curettes (Gracey group) according to a prospective, randomized, controlled, one-blind, "split-mouth" design. Twenty-six cases were available for the whole follow-up period. Plaque index (PLI), bleeding index(BI), probing depth (PD), attachment loss (AL) were evaluated before and 6 weeks after treatment. Treatment time was recorded. The severity of pain during treatment and teeth sensitivity after treatment were evaluated by the visual analogue scale (VAS). Differences in clinical parameters were analyzed with the Wilcoxon signed ranks test and Mann and Whitney U-test.No significant differences in any of the clinical parameters were observed at baseline between the two groups. The mean value of PD, BI, PLI, AL decreased in both ultrasonic group and Gracey group. At moderately deep site (initial PD between 4 mm and 5 mm), PD [M(Q(25), Q(75))] changed in the ultrasonic group from 4.0 (4.0, 4.5) mm to 3.0 (3.0, 3.0) mm (P0.001) and in the Gracey group from 4.0 (4.0, 5.0) mm to 3.0(3.0, 3.0) mm (P0.001). At deep sites (initial PD ≥ 6 mm) PD [M(Q(25), Q(75))] changed in the ultrasonic group from 7.0(6.0, 7.0) mm to 5.0(4.0, 7.0) mm (P0.001) and in the Gracey group from 7.0 (6.0, 7.0) mm to 5.0(4.0, 6.0) mm(P0.001). In the furcation area, PD [M(Q(25), Q(75))] changed from 5.0(4.0, 7.0) mm to 3.0(3.0, 5.0) mm (P0.001) in both Gracey group and ultrasonic group. However, the average time of active instrumentation was (2.41 ± 0.61) min/tooth in the ultrasonic scaling and (2.71 ± 0.61) min/tooth in the Gracey curette (P0.001). VAS scores [M(Q(25), Q(75))] of pain during treatment was 5.0(3.0, 6.7) in the ultrasonic group and 5.9 (4.9, 8.0) in the Gracey group (P = 0.001). VAS scores [M(Q(25), Q(75))] of sensitivity after treatment was 4.0 (1.8, 6.0) in the ultrasonic group and 4.9 (2.0, 8.0) in the Gracey group (P = 0.043).Treatment with the special series of ultrasonic inserts was as effective as the Gracey curette during initial therapy period in all clinical parameters measured and has the advantage of being quicker.

To evaluate the effect of locally applied bio-glass on the teeth hypersentivity and periodontal recovery after subgingival scaling and root planing.In this double blind study, 60 patients with chronic periodontitis were included and were randomly divided into four groups of 15 patients: bio-glass powder and bio-glass paste group (Po+Pa), bio-glass powder group (Po), bio-glass paste group (Pa) and control group. Powder was applied in pockets after subgingival scaling and root planning (SRP) and paste was used as toothpaste for 6 weeks. Periodontal indices and the severity of tooth hypersensitivity were recorded.Obvious mitigation of tooth hypersensitivity was observed in bio-glass powder or paste applied patients within the fist three weeks after SRP. Only few patients presented teeth hypersensitivity in the 6th week after SRP. In the 6th week after SRP, bleeding index significantly decreased in bio-glass powder or paste applied patients. More pocket depth and clinical attachment level reduction was found in bio-glass powder applied patients. Application of bio-glass powder or paste improved clinical attachment level in the 3rd month after SRP.The hypersensitivity after subgingival scaling could be relieved without any treatment in 6 weeks, while the bio-glass powder and the 7% bio-glass paste could reduce the prevalence and the severity of it. Further more, bio-glass also improved the clinical outcomes of subgingival scaling via its bacteriostatic action.

There were increasing studies on the association of periodontitis with preterm low birth weight (PLBW) in the recent 10 years. PLBW is associated with about half of all perinatal mortality. Known factors which could induce PLBW include smoking, genetics, alcohol, prenatal care, nutrition, urinary tract infections, However, there are still about 25% of PLBW cases occur with etiology unknown. Many evidences supported the association between periodontitis and PLBW. Periodontitis is a risk factor of PLBW, but the mechanism is unclear. Current theory suggests that periodontal inflammation adversely affects pregnancy outcomes by one or two mechanisms. First, women with periodontal disease may experience more frequent and severe bacteremia than periodontally healthy women. As a result, the uterine cavity may become exposed to or colonized by periodontal bacteria or their byproducts (e.g. lipopolysaccharides). Once they reach the maternal-fetal unit, oral bacteria may elicit an inflammatory cascade that leads to preterm labor. A second putative mechanism does not require oral bacteria to colonize the uterine cavity. Rather, cytokines generated within the diseased periodontal tissue may enter the systemic circulation and precipitate a similar cascade, again leading to spontaneous preterm labor and birth. Therefore, oral health instruction and periodontal treatment may decrease the infection of periodontal pathogens and reduce the risk of PLBW. For the present, the best advice for a woman who contemplate pregnancy is effective brushing for two times per day and regular periodontal treatment.

Molybdenum cofactor (Moco) is required for the activities of Moco-dependant enzymes. Cofactor for nitrate reductase and xanthine dehydrogenase (Cnx1) is known to be involved in the biosynthesis of Moco in plants. In this work, a soybean (Glycine max L.) Cnx1 gene (GmCnx1) was transferred into soybean using Agrobacterium tumefaciens-mediated transformation method. Twenty seven positive transgenic soybean plants were identified by coating leaves with phosphinothricin, bar protein quick dip stick and PCR analysis. Moreover, Southern blot analysis was carried out to confirm the insertion of GmCnx1 gene. Furthermore, expression of GmCnx1 gene in leaf and root of all transgenic lines increased 1.04-2.12 and 1.55-3.89 folds, respectively, as compared to wild type with GmCnx1 gene and in line 10 , 22 showing the highest expression. The activities of Moco-related enzymes viz nitrate reductase (NR) and aldehydeoxidase (AO) of T1 generation plants revealed that the best line among the GmCnx1 transgenic plants accumulated 4.25 μg g(-1) h(-1) and 30 pmol L(-1), respectively (approximately 2.6-fold and 3.9-fold higher than non-transgenic control plants).In addition, overexpression ofGmCnx1boosted the resistance to various strains of soybean mosaic virus (SMV). DAS-ELISA analysis further revealed that infection rate of GmCnx1 transgenic plants were generally lower than those of non-transgenic plants among two different virus strains tested. Taken together, this study showed that overexpression of a GmCnx1 gene enhanced NR and AO activities and SMV resistance, suggesting its important role in soybean genetic improvement.