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The ingestion of blood represents a significant burden that immediately increases water, oxidative, and thermal stress, but provides a significant nutrient source to generate resources necessary for the development of progeny. Thermal stress has been assumed to solely be a negative byproduct that has to be alleviated to prevent stress. Here, we examined if the short thermal bouts incurred during a warm blood meal are beneficial to reproduction. To do so, we examined the duration of pregnancy and milk gland protein expression in the tsetse fly, Glossina morsitans, that consumed a warm or cool blood meal. We noted that an optimal temperature for blood ingestion yielded a reduction in the duration of pregnancy. This decline in the duration of pregnancy is due to increased rate of blood digestion when consuming warm blood. This increased digestion likely provided more energy that leads to increased expression of transcript for milk-associated proteins. The shorter duration of pregnancy is predicted to yield an increase in population growth compared to those that consume cool or above host temperatures. These studies provide evidence that consumption of a warm blood meal is likely beneficial for specific aspects of vector biology.

Traditional Chinese medicines (TCMs) are highly valued and widely used worldwide. However, their complex compositions and various preparation processes have brought considerable challenges to the quality evaluation of Chinese medicines. The traditional methods for TCM quality evaluation suffer from the problems of cumbersome sample preparation, a long detection time, low sensitivity, etc. A more efficient and accurate evaluation method is urgently needed to ensure the stability and reliability of the quality of TCMs. As an emerging analytical technology, a fluorescent probe has the advantages of high sensitivity, high selectivity, easy operation, etc. It is capable of generating a specific fluorescent signal response to specific components in traditional Chinese medicines, realizing rapid and accurate detection of target components, which effectively solves the many difficulties of traditional methods. The purpose of this paper is to discuss the application of fluorescent probes in the quality evaluation of traditional Chinese medicines and the challenges they face. By introducing the principles, advantages and specific application cases of fluorescent probe technology in the quality evaluation of traditional Chinese medicines, we hope to provide new and efficient analytical ideas for the quality evaluation of traditional Chinese medicines. [ABSTRACT FROM AUTHOR]

Chloride penetration is one of the main causes of failure of reinforced concrete structures, especially in undersea tunnels, which is also accompanied by stress and seepage-induced calcium leaching. The chloride penetration resistance of concrete under the single and dual effects of stress and calcium leaching were investigated in this work. The microscopic mechanisms were evaluated using scanning electron microscopy and X-ray diffraction analysis. The results showed that the chloride diffusion depth and chloride content increased linearly with the stress level in the tensile zone and decreased linearly in the compressive zone; the change was more obvious in the tensile zone. At stress levels of 15%, 30% and 45%, the diffusion depth in the tensile zone increased by 11.4%, 36.6% and 43.5%, respectively, while the change ratios of chloride content were 1.2%, 11.2% and 19.4%. No significant synergistic effect between stress and calcium leaching on the chloride diffusion depth was found, but the effect on chloride content was obvious, especially when the tensile stress level was below 15% and the compressive stress level was above 30%, for which the differences caused by considering the synergistic effect or not were approximately 6% and 15%, respectively. [ABSTRACT FROM AUTHOR]

Polyhydroxyalkanoates (PHAs) are biodegradable plastics with great performance and development prospects. However, their traditional anaerobic/aerobic enrichment process requires a high concentration of dissolved oxygen (DO), resulting in high energy consumption. In this study, an anaerobic/oxygen-limited with secondary feeding enrichment mode was used to enhance the synthesis of PHAs while reducing energy consumption. The enrichment process of PHAs-synthesizing bacteria lasted up to 100 days, and the experiment was conducted to investigate the change of the PHAs synthesizing ability of the system in this mode by detecting the PHAs content and community distribution of the activated sludge under different stages. Under these conditions, the system enriched two major genera of PHAs-synthesizing bacteria, Thauera (30.21%) and Thiothrix (21.30%). The content of PHAs in the sludge increased from 4.51% to 30.87% and was able to achieve a concomitant increase in poly(3-hydroxyvalerate) (PHV) monomer content. After nitrogen limitation (C/N = 150) treatment, the content of PHAs reached 63.05%. The results showed that the enrichment mode of anaerobic/oxygen-limited with secondary feeding could enrich more PHAs-synthesizing bacteria and significantly increase the synthesis amount of PHAs, which revealed the great potential of this mode in solid waste value-added and reduce the production cost of PHAs and could provide a theoretical basis for the production of PHAs from activated sludge. [ABSTRACT FROM AUTHOR]

Tsetse flies are important vectors of human and animal trypanosomiasis. Ability to reduce tsetse populations is an effective means of disease control. Lactation is an essential component of tsetse's viviparous reproductive physiology and requires a dramatic increase in the expression and synthesis of milk proteins by the milk gland organ in order to nurture larval growth. In between each gonotrophic cycle, tsetse ceases milk production and milk gland tubules undergo a nearly two-fold reduction in width (involution). In this study, we examined the role autophagy plays during tsetse fly milk gland involution and reproductive output. Autophagy genes show elevated expression in tissues associated with lactation, immediately before or within two hours post-parturition, and decline at 24-48h post-parturition. This expression pattern is inversely correlated with that of the milk gland proteins (lactation-specific protein coding genes) and the autophagy inhibitor fk506-bp1. Increased expression of Drosophila inhibitor of apoptosis 1, diap1, was also observed in the milk gland during involution, when it likely prevents apoptosis of milk gland cells. RNAi-mediated knockdown of autophagy related gene 8a (atg8a) prevented rapid milk gland autophagy during involution, prolonging gestation, and reducing fecundity in the subsequent gonotrophic cycle. The resultant inhibition of autophagy reduced the recovery of stored lipids during the dry (non-lactating) periods by 15-20%. Ecdysone application, similar to levels that occur immediately before birth, induced autophagy, and increased milk gland involution even before abortion. This suggests that the ecdysteroid peak immediately preceding parturition likely triggers milk gland autophagy. Population modeling reveals that a delay in involution would yield a negative population growth rate. This study indicates that milk gland autophagy during involution is critical to restore nutrient reserves and allow efficient transition between pregnancy cycles. Targeting post-birth phases of reproduction could be utilized as a novel mechanism to suppress tsetse populations and reduce trypanosomiasis.

Polyhydroxyalkanoates(PHAs) are considered a good alternative to petroleum-based plastics because of their good biodegradability and biocompatibility. The synthesis of PHAs using activated sludge can not only solve the problem of the high cost of pure cultures but also improve the utilization value of activated sludge. In this study, sludge activity recovery experiments were firstly conducted and the effects of different initial sludge concentrations on the activated sludge PHAs synthesis system were further investigated. the initial sludge concentrations were 1#SBR (2800 ± 50) mg/L, 2#SBR (4200 ± 50) mg/L, and 3#SBR (5500 ± 50) mg/L. The results showed that the activity, sedimentation performance and PHAs synthesis capacity of activated sludge were enhanced after the sludge activity recovery experiment. At the initial sludge concentration of 4200 mg/L, the activated sludge PHAs synthesis system was operated stably and the synthesis efficiency of PHAs was enhanced. In contrast, at the initial sludge concentration of 2800 and 5500 mg/L, the steady state of the activated sludge PHAs synthesis system was damaged to different degrees at different times, and the synthesis efficiency of PHAs was greatly reduced. [ABSTRACT FROM AUTHOR]