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Introduction: Hepatocellular carcinoma (HCC) accounts for more than 90% of primary liver cancer. Although great progress has been made on HCC molecular mechanism and therapy techniques, the prognosis of HCC patient is poor due to high metastasis and recurrence. Materials and methods: Expression of miR-542-3p was quantified by quantitative real-time PCR (qRT-PCR). The role of miR-542-3p in HCC metastasis was examined using transwell and 3D-culture assay. qRT-PCR, Western blotting and luciferase reporter assay were used to elucidate the mechanisms of miR-542-3p-mediated cancer metastasis. Results and Conclusion: In the research, we found that miR-542-3p is decreased in HCC cell lines and tissues, and downregulation of miR-542-3p enhances, while upregulation suppresses HCC cell invasion ability. Further assay demonstrated that miR-542-3p can directly target TGF-β1 3' untranslated region (3'UTR) to influence TGF-β/Smad signaling pathway, and suppression of miR-542-3p can hyperactivate TGF-β/Smad pathway and further to promote Epithelial-Mesenchyme Transition (EMT) and induce poor prognosis. Lastly, the clinical correlation analysis illustrated that miR-542-3p is negatively related with the activity of TGF-β1. In summary, our results find that miR-542-3p takes an important role on HCC progression and provide more evidence of microRNAs (miRNAs) for cancer therapy. [ABSTRACT FROM AUTHOR]

PURPOSE: To establish the limited generation of cell line of human parotid pleomorphic adenoma (PA). METHODS: The sample was obtained from a male patient with parotid pleomorphic adenoma. Growth curve was made following the 7th passage cells. PA cell morphological characteristics were observed by phase- contrast invert microscope and identified by means of immunohistochemistry. RESULTS: PA cells were maintained in culture for over 2 months. PA cells were short spindle-shaped or polygonal in vitro. The antibody against cytokeratin was labelled in the cytoplasms of adenoepithelial cells, and myoepithelial cells were positive for the antibody against smooth muscle actin and vimentin. CONCLUSION: The culture of PA cells could continuously deliver passage in vitro, and can be used to prepared for establishing immortalized pleomorphic adenoma cell lines. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality(08JC 1412900) and Shanghai Leading Academic Discipline Project(S30206). [ABSTRACT FROM AUTHOR]