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Herein, a new colorimetric sensor array was developed for the first time, which can rapidly recognize 9 types of metal ions (e.g., Ni2+, Zn2+, Cd2+, Cu2+, Cr3+, Fe2+, Se2+, Mn2+, and Mg2+). The colorimetric characteristics of the sensor array were closely related to the oxidation etching of the triangular gold nanoplates (AuNPLs) by hydrogen peroxide (H2O2), catalyzed by horseradish peroxidase (HRP). In the design, two types of thiols (glutathione (GSH) and cysteine (Cys)) as recognition elements were employed to construct the sensor units (AuNPLs/GSH and AuNPLs/Cys) and adjust the etching degrees of AuNPLs in the presence of various metal ions. The differential binding affinities between metal ions and thiols will lead to different degrees of oxidation etching of AuNPLs with hydrogen peroxide, exhibiting characteristic colors, which can be visually distinguished by the naked eye. Thus, the colorimetric sensor array provides a new way for the discrimination of various metal ions, thereby simplifying the water quality analysis.

Sialic acid (SA), known as N-acetyl neuraminic acid, is a natural 9-carbomonosaccharide derivative. SA has been widely applied in the early diagnosis of diseases as therapeutic target. However, the abundance of SA is very low in biological samples, which is usually interfered by the similar molecules coexisting at high abundance. Combining the advantages of high selectivity and specificity of molecularly imprinted technology, high specific surface area of mesoporous materials and excellent optical properties of quantum dots, we chose Mn-doped ZnS quantum dots as signal elements, and sialic acid as the template molecule. KH-4-MAPB with recognition ability to SA was synthesized by one-step hydrothermal method using thiolene click reaction as functional monomer. Based on the principle of boron affinity, molecularly imprinted polymers with highly ordered mesoporous structure were prepared, and the structure and fluorescence properties of fluorescent molecularly imprinted polymers were studied. FT-IR, XRD, TEM and nitrogen adsorption-desorption experiments were used to characterize the structure and morphology of the molecularly imprinted polymers. The results showed that the prepared molecularly imprinted polymers had highly ordered mesoporous structure and a large number of imprinted holes, which ensured the specific selectivity of the molecularly imprinted polymers. The fluorescence properties of MIMPs were characterized and analyzed by fluorescence spectra, equilibrium adsorption kinetics experiments were conducted and imprinting properties were recorded under different pH. The above experimental results showed that the fluorescence quenching was successfully achieved when the template molecule SA was captured by the molecularly imprinted polymer. When the concentration of SA was 1.25-100 × 10

As the most active field of technological innovation, service innovation and business innovation, digital economy has entered the 2.0 era of rapid penetration of digital technology, deep concentration of industry and comprehensive integration of platforms driven by mobile Internet, big data, cloud computing, Internet of things and artificial intelligence. Digital economy has reshaped the music industry in terms of R & D, production and sales. From the perspective of digital economy, combined with the case of Netease Cloud Music, using the thinking mode of business model canvas, this paper analyzes the innovation of business model of Chinese music industry, and puts forward suggestions for the development of music industry.

This study was performed to analyze the potential resistant mutations within HBV reverse transcriptase (RT) sequences against nucleos(t)ide analogues (NA). HBV DNA RT region spanning from amino acid 169 to 250 was amplified and sequenced from 435 HBV patients who experienced NA treatment. Among study’s cohort, genotypes B and C infected patients were 55.9% and 44.1%, respectively. Mutations were recorded in 54.7% (238/435) patients at 22 positions. Genotype C displayed significant higher frequency of potential NA resistant mutations than genotype B (63.0% vs. 48.1%, P = 0.003). Moreover, eight mutation sites, including 180, 181, 191, 200, 202, 221, 229 and 224, in genotype C showed significant higher frequencies than in genotype B. In contrast, mutation at site 236 was more common in genotype B. Notably, 11 mutations at position 169, 202, 250, 173, 180, 200, 207, 214, 237, 242 and 245 coexisted with M204I or V. Substitutions at nine non-classical mutation sites (191, 207, 213, 218, 221, 224, 229, 238 and 242) were detected in patients with virological breakthrough. Particularly, tenofovir (TDF) resistance was observed in one patient undergoing TDF monotherapy and experienced several NA treatment before. These results might provide clinical useful information under antiviral therapy.

Heavy metals (e.g., arsenic (As)) and tetracycline (TC) usually coexist in wastewater from livestock farm, whereas the co-adsorption behaviours and mechanisms of As(V) and TC were not well-known. This study investigated the adsorption and co-adsorption of As(V) and TC by a novel yttrium-immobilized-graphene oxide-alginate hydrogel (Y-GO-SA) to explore the adsorption behaviours and mechanisms. The adsorption of As(V) and TC was pH-dependent. The maximum adsorption capacities under the studied concentrations were 273.39 mg/g for As(V), and 477.9 mg/g for TC, respectively, which are much higher than many other reported adsorbents. Furthermore, As(V) adsorption was due to ion exchange between hydroxyl groups and H2AsO42− groups and H-bonds formed with O-containing groups on Y-GO-SA, and the adsorption of TC by Y-GO-SA was mainly ascribed to electrostatic interaction, H-bonds, π − π EDA interaction, n-π EDA interaction, and cation-bonding bridge effects. The co-adsorption of As(V) and TC in binary system indicated that the presence of TC obviously suppressed the adsorption of As(V) due to the competition for active sites, whereas the effect of presence of As(V) on adsorption of TC can be negligible due to the balance contributions from its contrary effects, i.e. enhancement (anion-π interaction) and reduction (competition for Y ions) in TC adsorption. Finally, the hydrogels performed well in the treatment of livestock farm waste water. It can be anticipated that the prepared 3D hydrogel can be used as a powerful adsorbent in the practical application of waste water treatment, owing to its easy separation, high adsorption and good reusability.

Understanding how gene alterations induce oncogenesis plays an important role in cancer research and may be instructive for cancer prevention and treatment. We conducted a parameter sensitivity analysis to the mitochondrial apoptosis model. Both a nonlinear bifurcation analysis of the deterministic dynamics and energy barrier analysis of the corresponding stochastic models were performed. We found that the parameter sensitivity ranking according to the change of the bifurcation-point locations in deterministic models and the change of the barrier heights from a living to death state of the cell in stochastic models are highly correlated. For the model we considered, in combination with previous knowledge that the parameters significantly affecting the system's bifurcation point are strongly associated with frequently mutated oncogenic genes, we conclude that the energy barrier height can be used as indicator of oncogenesis as well as bifurcation point. We provide a possible mechanism that may help elucidate the logic of cancer initiation from the view of stochastic dynamics and energy landscape. And we show the equivalence of energy barrier height and bifurcation-point location in determining the parameter sensitivity spectrum for the first time.

Portal vein tumor thrombus (PVTT) in hepatocellular carcinoma (HCC) is a common entity. However, the prognosis of patients with hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) is extremely poor. The current study reports a case of HCC with PVTT, including a description of the gross surgical pathology and discussion of the relevance of assessing the growth of the tumor thrombus to the outcome of this disease. This case suggests that destruction of the primary tumor and PVTT must be considered according to the growth characteristics and modality of PVTT. It also indicates that transcatheter arterial embolization may be a suitable strategy for palliative treatment of patients with advanced HCC with PVTT.

It has long been known that formation of all sorts of tumors is largely owing to the genomic variations. Oncogenic mutations are often found focused on one or more important pathways which indicate that it is meaningful to investigate oncogenic mutations and oncogenic mechanisms from the point of view of biological network. Recently, we found that in apoptosis pathway of mammalian cell, mutations that cause large variations on the bifurcation point are more probably oncogenic mutations. Here, we used the Rb-E2F pathway in mammalian cell in response to growth factor as another example to verify this correlation. To conduct this study, nonlinear dynamics equations that describe the behavior of the Rb-E2F pathway was first constructed. Then we identified sensitive parameters which have a great influence on the system’s bifurcation point. And we found that the sensitive parameters are highly related to high-frequency oncogenic mutations after comparing the results of parameter sensitivity analysis with profile of known cancer mutations. Moreover, the position of bifurcation point rather than concentration of a certain protein is a better measurement to determine biological network's function. Our results further confirm that nonlinear dynamics analysis of biological networks is an important way to understand oncogenesis. And the analysis method can become a powerful tool to understand and analyze the function of biological network.

Background Hepatitis B e Antigen (HBeAg)-negative chronic hepatitis B (CHB) patients have an active liver disease with a high risk of progression to decompensated cirrhosis and hepatocellular carcinoma. The management strategy for HBeAg-negative CHB patients treated with nucleos(t)ide analogues (NUCs) is a topic of concern. To observe the outcomes for this population after NUCs withdrawal, HBeAg-negative CHB patients with loss of hepatitis B surface antigen (HBsAg) or sustained undetectable HBV DNA levels who had discontinued NUCs therapy were included in the study. Methods A total of 66 patients (2 patients with HBsAg loss and 64 patients with sustained undetectable HBV DNA levels) were examined. HBV DNA levels and alanine aminotransferase (ALT) levels were monitored regularly after discontinuation of NUCs therapy. Relapse was defined as HBV DNA levels >2,000 IU/mL while off therapy in at least two determinations more than 4 weeks apart. Results The time to achieve undetectable HBV DNA levels was 14 weeks (interquartile range (IQR): 12–24 weeks). The time until consolidation therapy was 144 weeks (IQR: 96–168 weeks). No relapses occurred in either of the HBsAg loss patients. Among the 64 patients with undetectable HBV DNA levels, 19 (29.7%) patients demonstrated evidence of relapse. All the relapses occurred within 96 weeks after discontinuation. The median duration of relapse was 36 weeks (IQR: 12–48 weeks). Elevation of HBV DNA and ALT levels over baseline was only observed in 10% of the relapse patients. There were no significant differences among the baseline characteristics (sex, HBV genotype, age, or ALT level) or the time until consolidation therapy between relapse and sustained-response patients. Conclusions NUC discontinuation is feasible after achieving undetectable HBV DNA levels in HBeAg-negative CHB patients. Prolonging the time until consolidation therapy may be a good strategy to decrease the rate of relapse. More than 96 weeks of sustained response is a predictive marker of long-term sustained response.

For the condition of urban non revenue water in our country, this paper on the base of the scientific analysis of evaluation of the condition of urban non revenue water at home and abroad provides evaluation index system, which composed of seven indexes divided into 3 groups: physical loss or real loss, apparent loss and unbilled authorized consumption, and builds an evaluation model based on BP neural network. Though non-linear mapping function, evaluation indexes are standardized, and this paper combining with examples proves that the method is simple, accurate and effective verify the effectiveness of the method.

To investigate the relationship between impaired fracture healing and the expression of peroxisome proliferator activated-receptor gamma (PPARgamma) and core binding factor alpha 1 (Cbfalpha1) mRNA in the bone marrow of Type 2 diabetic rats.Thirty male Sprague-Dawley rats were divided into a control group (n=15, normal diet) and an experiment group (n=20, high fat and sucrosum diet). After 8 weeks, one eyeball was culled for blood collection. The experiment group was intraperitoneally injected with streptozotocin and the control group received citrate buffer. After another 2 weeks, the other eyeball was culled again to collect blood. The model of distraction osteogenesis in the left tibias of the rats was established. After 14 days, we sacrificed all the rats and collected the blood and left tibias. Both femurs were harvested with germ free. We observed the callus formation in the distraction gap by X-ray and the formation of primary matrix front and microcolumn by histological examination. We observed adipocytes in adjacent bone marrow of left tibia and computed the percentage of adipocytes accounting for the area of bone cavity. We measured the expression of PPARgamma and Cbfalpha1 mRNA in femurs marrow by RT-PCR.In the experimental rats, the level of triglyceride was obviously elevated (P0.01), so was the total cholesterol (P0.05), while the level of fasting blood glucose and serum insulin did not obviously differ (P0.05) after 8 weeks. The level of fasting blood glucose and triglyceride was obviously elevated (P0.01), and so was the total cholesterol (P0.05), while the level of serum insulin was not obviously different in the experiment group after 10 and 12 weeks. Callus formation in the distraction gap was obviously diminished by X-ray in the experimental rats. The array of microcolumn formation was disordered and the area of primary matrix front was catachromasis in the controls by histology examination. The number of adipocytes in both the bone marrow and the percentage of adipocyte accounting for the area of bone cavity increased (P0.01) in the experiment group. In the experiment group, the expression of PPARgamma mRNA was up-regulated (P0.05) while Cbfalpha1 mRNA was down-regulated (P0.05) in double femur marrow.Increased expression of PPARgamma mRNA and decreased expression of Cbfalpha1 mRNA in the bone marrow may cause impaired fracture healing in rats with Type 2 diabetes.

This paper deals with the optimal tracking control (OTC) problem for a class of nonlinear system with control- affine form. The feedforward compensation technique is used in order to make the system's output asymptotically track the desired trajectory. An adjoint vector is introduced to compensate the control-affine nonlinearity of the dynamic system. The compensation term is obtained by introducing an iterative procedure for solving a sequence of linear inhomogeneous two- point boundary value (TPBV) problems using the successive approximation approach (SAA). The OTC law obtained consists of an accurate state feedback term, a feedforward term and a compensation term which is the limit of the adjoint vector sequence. Using a finite-time iteration of the adjoint vector among optimal solution sequence, we can obtain a suboptimal tracking control (STC) law. An aircraft model is employed to test the validity of this method.

Clotrimazole, which is an imidazole derivative antifungal agent, was widely used for the treatment of mycotic infections of the genitourinary tract. To develop alternative formulation for the vaginal administration of clotrimazole to provide sustained and controlled release of appropriate drug for local vaginal therapy, liposomes/niosomes were evaluated as delivery vehicles. To optimize the preparation of liposomes/niosomes with regard to size and entrapment efficiency, multilamellar liposomes/niosomes containing drug were prepared by lipid hydration method. The prepared liposomes/niosomes were incorporated into 2% carbopol gel, and the systems were evaluated for drug stability in phosphate-buffered saline (pH 7.4) and simulated vaginal fluid at 37 +/- 1 degrees C. Further, the vesicle gel system was evaluated by antifungal activity and tolerability on tissue level in rat.