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17 results on '"Xiang, Charlie C."'

8. Sensitive and specific method for detecting G protein–coupled receptor mRNAs

Author

Hansen, Arne, primary, Chen, Yidong, additional, Inman, Jason M, additional, Phan, Quang N, additional, Qi, Zhi-Qing, additional, Xiang, Charlie C, additional, Palkovits, Miklos, additional, Cherman, Natasha, additional, Kuznetsov, Sergei A, additional, Robey, Pamela G, additional, Mezey, Eva, additional, and Brownstein, Michael J, additional

Published
2006
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10. Regulation of bile acid biosynthesis by hepatocyte nuclear factor 4α

Author

Inoue, Yusuke, primary, Yu, Ai-Ming, additional, Yim, Sun Hee, additional, Ma, Xiaochao, additional, Krausz, Kristopher W., additional, Inoue, Junko, additional, Xiang, Charlie C., additional, Brownstein, Michael J., additional, Eggertsen, Gösta, additional, Björkhem, Ingemar, additional, and Gonzalez, Frank J., additional

Published
2006
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13. Preparing Fluorescent Probes for Microarray Studies.

Author

Walker, John M., Khodursky, Arkady B., Xiang, Charlie C., and Brownstein, Michael J.

Abstract

A number of articles have been published describing methods to produce fluorescent probes from RNA (or DNA) samples. These methods are conceptually similar. Broadly speaking, they involve some or all of the following procedures: template amplification, template transcription with concomitant incorporation of modified bases into the transcribed products (DNA or RNA), and signal amplification. The simplest technique relies on the direct incorporation of Cy3- or Cy5-labeled nucleotides into oligo-dT-primed cDNA by reverse transcriptase (RT). This method has proven to be reasonably robust, but it requires 50-200 μg of total RNA or 2-5 μg of poly(A) RNA per labeling (1-4). Thus, rather large tissue samples are required for each study. [ABSTRACT FROM AUTHOR]

Published
2003
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14. Fabrication of cDNA Microarrays.

Author

Walker, John M., Khodursky, Arkady B., Xiang, Charlie C., and Brownstein, Michael J.

Abstract

DNA microarray technology has been used successfully to detect the expression of many thousands of genes, to detect DNA polymorphisms, and to map genomic DNA clones (1-4). It permits quantitative analysis of RNAs transcribed from both known and unknown genes and allows one to compare gene expression patterns in normal and pathological cells and tissues (5,6). [ABSTRACT FROM AUTHOR]

Published
2003
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15. Sensitive and specific method for detecting G protein–coupled receptor mRNAs.

Author

Hansen, Arne, Yidong Chen, Inman, Jason M., Phan, Quang N., Zhi-Qing Qi, Xiang, Charlie C., Palkovits, Miklos, Cherman, Natasha, Kuznetsov, Sergei A., Robey, Pamela G., Mezey, Eva, and Brownstein, Michael J

Subjects
G proteins, MEMBRANE proteins, MESSENGER RNA, MOLECULES, BONE marrow, HEMATOPOIETIC system, IMMUNE system
Abstract

G protein–coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 μg of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines. [ABSTRACT FROM AUTHOR]

Published
2007
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16. Fabrication of cDNA microarrays.

Author

Xiang CC and Brownstein MJ

Subjects
Animals, Cloning, Molecular, DNA, Complementary, Humans, Mice, Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis instrumentation, Oligonucleotide Array Sequence Analysis methods
Published
2003
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17. Preparing fluorescent probes for microarray studies.

Author

Xiang CC and Brownstein MJ

Subjects
DNA, Complementary biosynthesis, DNA, Complementary isolation & purification, RNA isolation & purification, Fluorescent Dyes chemical synthesis, Oligonucleotide Array Sequence Analysis instrumentation, Oligonucleotide Array Sequence Analysis methods
Published
2003
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