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9 results on '"Xia, Libing"'

3. Safety evaluation of FAD2 RNAi transgenic Brassica napus L. based on microbial diversity and metabonomic analysis

Author

Qi, Yanting, Wang, Qiming, Xie, Qingxuan, Wu, Chuan, Xu, Minhui, Han, Shaofan, Zhou, Ting, Li, Juan, Xia, Libing, Li, Wai chin, and Pan, Weisong

Subjects
Plant Science
Abstract

Oleic acid desaturase (FAD2) is the key enzyme that produces polyunsaturated fatty acids in rapeseed (Brassica napus L), which is one of the main oil crops. RNA interference (RNAi) is an emerging technique that provides new opportunities for the generation of new traits in plants. To increase oleic acid content and reduce linoleic and linolenic acid content in rapeseed, we constructed an ihpRNA plant expression vector of the FAD2 gene and obtained transgenic plants for multiple generations by stable inheritance. In this study, third-generation transgenic plants (T3), seventh-generation transgenic plants (T7), and wild-type plants (WT) were used. The differences in microbial community diversity between transgenic plants and wild-type plants and the up- and downregulation of rhizosphere metabolite contents were investigated. In conclusion, the results showed that the soil microbial community structure was stable, the general microbial community structure was not changed by the transgenic rhizosphere exudates, and no significant harmful root exudate of transgenic rapeseed on the environment was found through the microbial community and metabolomics analysis. This work may provide an understanding of the impact of RNAi on plant metabolites and a safety evaluation method for transgenic plants and a reference for rapeseed breeding.

Published
2022
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9. Endoplasmic reticulum protein 29 is involved in endoplasmic reticulum stress in islet beta cells.

Author

Gao J, Zhang Y, Wang L, Xia L, Lu M, Zhang B, Chen Y, and He L

Subjects
Animals, Down-Regulation drug effects, Endoplasmic Reticulum Chaperone BiP, Gene Knockdown Techniques, HSP70 Heat-Shock Proteins genetics, HSP70 Heat-Shock Proteins metabolism, Heat-Shock Proteins genetics, Insulin-Secreting Cells drug effects, Membrane Proteins genetics, Membrane Proteins metabolism, Mice, Models, Biological, NIH 3T3 Cells, Protein Transport drug effects, RNA, Messenger genetics, RNA, Messenger metabolism, Tunicamycin pharmacology, Endoplasmic Reticulum Stress drug effects, Endoplasmic Reticulum Stress genetics, Heat-Shock Proteins metabolism, Insulin-Secreting Cells metabolism
Abstract

Endoplasmic reticulum stress (ERS) is correlated with insulin resistance and islet‑cell function. In the present study, the sub‑cellular localization and role of ER protein 29 (Erp29) were investigated in an in vitro ERS model of islet beta cells. The INS‑1 islet cell line was treated with various concentrations of tunicamycin to establish the ERS model. Immunofluorescence microscopy demonstrated that Erp29 and anti‑ER marker protein calnexin were co‑localized in NIH3T3 cells, suggesting that Erp29 is localized to the ER. The ERS model induced by tunicamycin showed significantly increased expression of binding immunoglobulin protein (BIP)/glucose‑regulated protein 78 (Grp78), which is a marker for ERS, and the expression of Erp29 was also increased at the mRNA and protein levels. Of note, ERS was blocked following small interfering RNA‑mediated silencing of Erp29 expression, as indicated by reduced BIP/Grp78 expression. As an ER protein, Erp29 may have an important role in ERS in islet beta cells.

Published
2016
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