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To evaluate the efficacy and safety of fecal microbiota transplantation (FMT) in functional gastrointestinal disorders (FGIDs) in children with abdominal bloating and changes in their gut microbiome and metabolome.Twelve pediatric FGID patients with predominant abdominal bloating who underwent FMT were enrolled in the study. Fourteen healthy controls and four stool donors were included for analysis. Clinical responses were assessed at 8 weeks after FMT. Fecal bacterial composition was determined by 16S rRNA gene sequencing. The fecal metabolome was measured by targeted metabolomics analysis.Median age of the 12 children with FGIDs was 6 years, and nine were boys. Abdominal bloating was relieved in all patients by FMT at 8 weeks. Meanwhile, FMT significantly improved abdominal pain and diarrhea. The a diversity was significantly lower in the FGID patients, while the fecal microbial community (β diversity) separated from that of healthy control (HCs). The relative abundances of multiple bacterial genera were significantly changed in the feces of the pediatric FGID patients. The levels of several short-chain fatty acids were lower, and lactic acid level was higher in FGID patients than in HCs. Altered bacterial composition was correlated with changes in the fecal metabolite profile and clinical symptoms in FGID patients. FMT modulated fecal microbiome and metabolome in FGID children toward a healthy state.FMT relieves abdominal bloating and modulates fecal microbiome and metabolome toward a healthy state in children with FGIDs. FMT may provide an alternative therapy for children with FGIDs and abdominal bloating.

Natural product dimers have intriguing structural features and often have remarkable pharmacological activities. We report here two uncommon marine gorgonian-derived symmetric dimers, weizhouochrones A (

Background Acute myeloid leukemia (AML) is a myeloid neoplasm accounts for 7.6% of hematopoietic malignancies. AML is a complex disease, and understanding its pathophysiology is contributing to the improvement in the treatment and prognosis of AML. In this study, we assessed the expression profile and molecular functions of CCAAT enhancer binding protein gamma (CEBPG), a gene implicated in myeloid differentiation and AML progression. Methods shRNA mediated gene interference was used to down-regulate the expression of CEBPG in AML cell lines, and knockdown efficiency was detected by RT-qPCR and western blotting. The effect of knockdown on the growth of AML cell lines was evaluated by CCK-8. Western blotting was used to detect PARP cleavage, and flow cytometry were used to determine the effect of knockdown on apoptosis of AML cells. Genes and pathways affected by knockdown of CEBPG were identified by gene expression analysis using RNA-seq. One of the genes affected by knockdown of CEBPG was Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), a known repressor of translation. Knockdown of EIF4EBP1 was used to assess its potential role in AML progression downstream of CEBPG. Results We explored the ChIP-Seq data of AML cell lines and non-AML hematopoietic cells, and found CEBPG was activated through its distal enhancer in AML cell lines. Using the public transcriptomic dataset, the Cancer Cell Line Encyclopedia (CCLE) and western blotting, we also found CEBPG was overexpressed in AML. Moreover, we observed that CEBPG promotes AML cell proliferation by activating EIF4EBP1, thus contributing to the progression of AML. These findings indicate that CEBPG could act as a potential therapeutic target for AML patients. Conclusion In summary, we systematically explored the molecular characteristics of CEBPG in AML and identified CEBPG as a potential therapeutic target for AML patients. Our findings provide novel insights into the pathophysiology of AML and indicate a key role for CEBPG in promoting AML progression.

5-Methyl-cytosine (5mC) is one of the most important DNA modifications and plays versatile biological roles. It is well known that 5mC stabilizes DNA duplexes. However, it remains unclear how 5mC affects the kinetics of DNA melting and hybridization. Here, we studied the kinetics of unzipping and rezipping using a 502-bp DNA hairpin by single-molecule magnetic tweezers. Under constant loading rates, 5mC increases the unzipping force but counterintuitively decreases the rezipping force at various salt and temperature conditions. Under constant forces, the non-methylated DNA hops between metastable states during unzipping and rezipping, which implies low energy barriers. Surprisingly, the 5mC DNA can’t rezip after fully unzipping unless much lower forces are applied, where it rezips stochastically in a one-step manner, which implies 5mC kinetically hinders DNA hybridization and high energy barriers in DNA hybridization. All-atom molecular dynamics simulations reveal that the 5mC kinetically hinders DNA hybridization due to steric effects rather than electrostatic effects caused by the additional methyl groups of cytosines. Considering the possible high speed of DNA unzipping and zipping during replication and transcription, our findings provide new insights into the biological roles of 5mC.

Background: In recent years, non-invasive brain stimulation (NIBS) has often been used for therapeutic effects on motor functions. However, there is no clear evidence for the efficacy of NIBS in populations with spinal cord injury (SCI). This study aims to conduct a meta-analysis to summarize the existing evidence on the effectiveness and safety of NIBS for motor dysfunction after a spinal cord injury to obtain new insights. Methods: Two investigators systematically screened English articles from PubMed, MEDLINE, Embase, and Cochrane Library for eligible Randomized and prospective controlled trials regarding the effects of NIBS in motor function recovery after SCI. Studies with at least three sessions of NIBS were included. We assessed the methodological quality of selected studies as described in the evidence-based Cochrane Collaboration’ tool. A meta-analysis of the motor function was performed by pooling thestandardized mean difference (SMD)with 95% confidence intervals (CI). Results: A total of 14 studies involving 225 patients were included. Nine studies used repetitive transcranial magnetic stimulation (rTMS) and 5 studies used transcranial direct current stimulation (tDCS). The meta-analysis showed that NIBS could improve the lower extremity strength (SMD = 0.58, 95% CI= 0.02 to 1.14, P = 0.004), balance (SMD = 0.64, 95% CI= 0.05 to 1.24, P= 0.03) and decrees the spasticity (SMD = -0.64, 95% CI=-1.20 to-0.03, P =0.04); while functional mobility of the NIBS group was not statistically significant than that of the control group (upper extremity strength: P=0.07; spasticity: P=0.12). The motor function of upper extremity strength in the NIBS group did not reach statistical significance when compared with the sham group. Only one patient reported seizures occurred during stimulation; no other serious adverse events were reported. Conclusion: NIBS appears to have a positive effect on motor function of the lower extremity in SCI patients. However, the marginal p-value and a high heterogeneity suggest that these results should be interpreted with caution. Further high-quality clinical trials are needed to support or refute the use and optimize the stimulation parameters of NIBS in clinical practice.

Backgroud Our previous studies have reported electroacupuncture(EA) can regulate the relevant indicators of essential hypertension(EH) such as angiotensinII(ANGII),ANG(1-7) and inflammatory cytokines.But the mechanism of EA remains unclear. The goal of this study was to verify P2RX7 as one direct target of miRNA-9,which triggers the release of ANGII and PICs(TNF-α, IL-6)in the PVN.The system of miRNA-9/P2RX7 might be a novel mechanism of lowering blood pressure.Method Spontaneously Hypertensive Rats(SHRs) were selected as an animal model of hypertension while WKY rats were considered as a blank group.Bipolar cannula implantation were used to inject inhibitors in the paraventricular nucleus(PVN).We measured the systolic blood pressure(SBP) of animals on different days of acupuncture therapy(0,3,7,10,14).We examined the level of microRNA-9 by RealTime-PCR.RT-PCR and western blot was utilized to detect the mRNA and protein levels of P2RX7 in each group.The activation of microglias was detected by immunohistochemistry staining with antibody Iba1.ANGII,IL-6 and TNF-α were detected by ELISA in the PVN and plasma.Results Our results showed that EA reduces systolic blood pressure of SHRs.EA increased the level of miR-9 notably comparing to sham groups.In addition,SHRs in the EA group had significantly lower P2RX7 protein and mRNA levels than those in the sham group,while miR9-inhibitor downregulated the expression of P2RX7 in the PVN comparing to EA groups. Immunohistochemistry staining showed that EA treatment and P2RX7 inhibitor significantly inhibited the activation of microglia.We also found that the combination of P2RX7-inhibitor and EA had a better anti-inflammatory effect and better inhibition of ANGII,while using miR9-inhibitor prevented the effect.Conclusion Taken together, our data demonstrates that P2RX7 is a direct downstream receptor of miRNA-9.Regulating system, miRNA-9/P2XR7, decreases the release of ANGII and alleviates inflammation in the PVN and plasma of SHRs,which indicates a novel microRNA-mediated mechanism of EA.Furthermore, the present investigation provides the combination treatment of P2RX7-inhibitor and EA as a better intervention in the area of anti-inflammatory.

Recent studies uncovered the emerging roles of SAPCD2 (suppressor anaphase-promoting complex domain containing 2) in several types of human cancer. However, the functions and underlying mechanisms of SAPCD2 in the progression of neuroblastoma (NB) remain elusive. Herein, through integrative analysis of public datasets and regulatory network of GSK-J4, a small-molecule drug with anti-NB activity, we identified SAPCD2 as an appealing target with a high connection to poor prognosis in NB. SAPCD2 promoted NB progression in vitro and in vivo. Mechanistically, SAPCD2 could directly bind to cytoplasmic E2F7 but not E2F1, alter the subcellular distribution of E2F7 and regulate E2F activity. Among the E2F family members, the roles of E2F7 in NB are poorly understood. We found that an increasing level of nuclear E2F7 was induced by SAPCD2 knockdown, thereby affecting the expression of genes involved in the cell cycle and chromosome instability. In addition, Selinexor (KTP-330), a clinically available inhibitor of exportin 1 (XPO1), could induce nuclear accumulation of E2F7 and suppress the growth of NB. Overall, our studies suggested a previously unrecognized role of SAPCD2 in the E2F signaling pathway and a potential therapeutic approach for NB, as well as clues for understanding the differences in subcellular distribution of E2F1 and E2F7 during their nucleocytoplasmic shuttling.

A series of recyclable epoxy resin (REP) composites are successfully prepared in this study, aiming to improve flame retardancy while keep good recyclability and mechanical properties. The commercial 9,10-Dihydro-9-oxa-10-phosphaphenanthrene 10-Oxide (DOPO) with high reactive P-H bond is used as highly-efficient flame retardant in this work. Compared with pure epoxy resin with a limited oxygen index of 21.7 % and no rating in vertical burning test, recyclable epoxy resin only with 3 wt% DOPO acquires a limited oxygen index value of 27.8% and V-0 rating. In addition, REP composites containing 4 wt% DOPO displays quick self-extinguishing behavior, which shows the 43.5% drop of the peak heat release rate. The main reason might be that traditional phosphorous-containing flame retardant existing in REP composites can play a positive role in both condensed phase and gas phase when burning. On the other hand, both scanning electron microscopy and Raman results also further affirm the improved flame retardancy. Moreover, flame-retardant REP composites can also maintain good transparency and mechanical properties. As well as the favorable recyclability originated from the special exchangeable disulfide crosslinks. High-Performance REP composites possessing expected flame retardancy, recyclability and good mechanical properties pave the way to broaden applications of thermoset epoxy materials.

A detailed chemical investigation of the Chinese soft coral Lemnalia flava yielded four new nardosinane-type sesquiterpenoids (1–4), one new neolemnane-type sesquiterpenoid (5), and one new sesquiterpenoid with an uncommon 6/9 fused bicyclic skeleton (6), together with two known related compounds (7 and 8). The structures and absolute configurations of 1–8 were determined on the basis of extensive spectroscopic data analyses, X-ray diffraction analysis, chemical reactions, and computer-assisted structural elucidation including 13C NMR data calculation, residual dipolar coupling based NMR analysis, and time-dependent density functional theoryelectronic circular dichroism calculation. Plausible biogenetic pathways of two uncommon sesquiterpenoids (4 and 6) were proposed.

A novel diterpenoid, sarcomililate A (1), possessing a previously undescribed tricyclo[11.3.0.02,16]hexadecane scaffold, along with two new cembranoids, sarcomililatols A and B (2 and 3), and two known related diterpenoids (4 and 5), was isolated from the Hainan soft coral Sarcophyton mililatensis. The complete chemical structure including absolute configuration (AC) of 1 was unambiguously determined by a combination of residual dipolar coupling (RDC)-based NMR analysis, TDDFT-ECD (ECD = electronic circular dichroism) calculation, and Snatzke's method. The AC of 1 was further confirmed by a comparison with 2, whose AC was determined by anomalous X-ray diffraction. A plausible biogenetic relationship of 1-3 was further proposed. All the reported compounds exhibited interesting inhibitory effects on the ConA-induced T lymphocytes and/or lipopolysaccharide (LPS)-induced B lymphocytes proliferation.

BACKGROUND Fecal microbiota transplantation (FMT) is the administration of fecal bacterial liquid from healthy donors to a recipient’s digestive tract, which is recommended as a therapeutic method for recurrent Clostridium difficile infection (CDI). Many clinical trials focusing on different diseases are in progress. To date, scarce research and long-term follow-up have been conducted on FMT in children or on the proper guidelines. Our center first performed FMT to treat a 13-month-old boy with severe CDI in 2013. Until February 2018, our center had performed 114 pediatric FMT procedures in 49 subjects. AIM To investigate the safety of FMT in children. METHODS A retrospective study was conducted on 49 patients who underwent 114 FMT treatments at our hospital. All FMT processes followed uniform standards. Adverse events (AEs) related to FMT were divided into short-term (48 h post-FMT) and long-term (3 mo). All potential influencing factors for AEs, such as gender, age, time of FMT infusion, route of administration, disease type, immune function state, and donor relative genetic background, were analyzed as independent factors. The significant independent factors and risk ratio with 95% confidence interval (CI) were assessed by multivariate logistic regression analysis. RESULTS Forty-nine patients (mean age 68.1 mo, range 4 to 193 mo) were recruited. Their average follow-up time after the first FMT was 23.1 mo. The incidence of short-term AEs was 26.32% (30/114). The most common short-term AEs were abdominal pain, diarrhea, fever, and vomiting, which were all self-limited and symptom-free within 48 h. Two severe AEs occurred, and one patient died in the fourth week after FMT. All-cause mortality was 2.04%. As independent factors, age (P = 0.006) and immune state (P = 0.002) had significant effects. Age greater than 72 mo seemed to be correlated with more AEs than age 13 to 36 mo (P = 0.04). In multivariate logistic regression analysis, immune state was an independent risk factor for AE occurrence (P = 0.035), and the risk ratio in immunodeficient patients was 3.105 (95%CI: 1.080-8.923). CONCLUSION Although FMT was proven to be tolerated in children, we need to be more cautious with immunodeficient patients. The effect on children’s long-term health is unpredictable.

Five novel biscembranoids, ximaolides H–L (1–5), along with four known related compounds (6–9) were isolated from the Hainan soft coral Sarcophyton tortuosum. The structures of the new compounds were determined by extensive spectroscopic analysis, quantum chemical calculations, and/or by comparing their CD spectra with those of the known compounds. Compounds 1 and 2 are the first examples of biscembranoids bearing a 1, 35-bridged lactone moiety, 4 is the first biscembranoid comprising an uncommon oxetane ring, and 5 represents the first 36-peroxyl biscembranoid. Ximaolides I (2), K (4) and F (9) exhibited interesting anti-inflammatory activity by the inhibition of LPS-induced TNF-α protein release in RAW264.7 macrophages., This research work was financially supported by the National Natural Science Foundation of China (NSFC, Nos. 81991521, 8202290170, 42076099), the NSFC/CNRS (Centre national de la recherche scientifique) Joint Project (No. 81811530284), the National Key Research and Development Program of China (No. 2018YFC0310903), and the SKLDR/ SIMM Project (No. SIMM1903ZZ-04). X.-W. Li is also thankful for the Shanghai Rising-Star Program (No. 20QA1411100) and SA-SIBS Scholarship Program. We thank Prof. X.-B. Li from Hainan University for the taxonomic identification of the soft coral material.

Obtaining a well balance between the high fire safety and mechanical performance of epoxy resins (EP) is still a challenge for the traditionally intumescent flame retardant. Herein, a new strategy was constructed to realize the highly uniform dispersion of zeolitic imidazolate framework-67 (ZIF-67) on the ammonium polyphosphate (APP) through the coordination reaction of the pre-bonded Co2+ cations with phosphate and 2-methyimidazole. The as-obtained ZIF-67@APP in the loading of 5 wt% endowed EP with a LOI value of 28.5% and UL-94 V0. Furthermore, peak of heat release rate (PHRR) and smoke production rate (SPR) of ZIF-67@APP/EP were greatly decreased by 67.4% and 46.2% compared to EP with 5 wt% APP. The improved fire safety of ZIF-67@APP/EP was ascribed to the faster formation of the expanded char owing to the catalysis of cobalt cation. Meanwhile, the good compatibility between ZIF-67@APP and EP also presented better mechanical properties. pre-print 1806 KB

Background T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease with a high risk of induction failure and poor outcomes, with relapse due to drug resistance. Recent studies show that bromodomains and extra-terminal (BET) protein inhibitors are promising anti-cancer agents. ARV-825, comprising a BET inhibitor conjugated with cereblon ligand, was recently developed to attenuate the growth of multiple tumors in vitro and in vivo. However, the functional and molecular mechanisms of ARV-825 in T-ALL remain unclear. This study aimed to investigate the therapeutic efficacy and potential mechanism of ARV-825 in T-ALL. Methods Expression of the BRD4 were determined in pediatric T-ALL samples and differential gene expression after ARV-825 treatment was explored by RNA-seq and quantitative reverse transcription-polymerase chain reaction. T-ALL cell viability was measured by CCK8 assay after ARV-825 administration. Cell cycle was analyzed by propidium iodide (PI) staining and apoptosis was assessed by Annexin V/PI staining. BRD4, BRD3 and BRD2 proteins were detected by western blot in cells treated with ARV-825. The effect of ARV-825 on T-ALL cells was analyzed in vivo. The functional and molecular pathways involved in ARV-825 treatment of T-ALL were verified by western blot and chromatin immunoprecipitation (ChIP). Results BRD4 expression was higher in pediatric T-ALL samples compared with T-cells from healthy donors. High BRD4 expression indicated a poor outcome. ARV-825 suppressed cell proliferation in vitro by arresting the cell cycle and inducing apoptosis, with elevated poly-ADP ribose polymerase and cleaved caspase 3. BRD4, BRD3, and BRD2 were degraded in line with reduced cereblon expression in T-ALL cells. ARV-825 had a lower IC50 in T-ALL cells compared with JQ1, dBET1 and OTX015. ARV-825 perturbed the H3K27Ac-Myc pathway and reduced c-Myc protein levels in T-ALL cells according to RNA-seq and ChIP. In the T-ALL xenograft model, ARV-825 significantly reduced tumor growth and led to the dysregulation of Ki67 and cleaved caspase 3. Moreover, ARV-825 inhibited cell proliferation by depleting BET and c-Myc proteins in vitro and in vivo. Conclusions BRD4 indicates a poor prognosis in T-ALL. The BRD4 degrader ARV-825 can effectively suppress the proliferation and promote apoptosis of T-ALL cells via BET protein depletion and c-Myc inhibition, thus providing a new strategy for the treatment of T-ALL.

Understanding the processes of immune regulation in patients infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for improving treatment. Here, we performed longitudinal whole-transcriptome RNA sequencing on peripheral blood mononuclear cell (PBMC) samples from 18 patients with coronavirus disease 2019 (COVID-19) during their treatment, convalescence, and rehabilitation. After analyzing the regulatory networks of differentially expressed messenger RNAs (mRNAs), microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) between the different clinical stages, we found that humoral immunity and type I interferon response were significantly downregulated, while robust T-cell activation and differentiation at the whole transcriptome level constituted the main events that occurred during recovery from COVID-19. The formation of this T cell immune response might be driven by the activation of activating protein-1 (AP-1) related signaling pathway and was weakly affected by other clinical features. These findings uncovered the dynamic pattern of immune responses and indicated the key role of T cell immunity in the creation of immune protection against this disease.

Determination of the stereochemistry of organic molecules still represents one of the major obstacles in the structure elucidation procedure in drug discovery. Although the application of residual dipolar couplings (RDCs) has revolutionized this field, residual chemical shift anisotropies (RCSAs) which contain valuable structural information for nonprotonated carbons have only been scarcely employed so far. In this study, we present a simple but highly effective solution to extract RCSAs of the analytes in a liquid crystalline phase formed by AAKLVFF oligopeptides. This method does not require any special instruments, devices, or correction during postacquisition data analysis and thus can be easily applied in any chemistry laboratory. To illustrate the potential of this method, the relative configurations of four known natural products (1-4) belonging to different structural classes were confirmed. Moreover, we unambiguously elucidated the stereochemistry of spiroepicoccin A (5), a rare thiodiketopiperazine marine natural product whose configuration could not be assigned based on conventional NMR methods.

Urban rail stations play an important role in passenger distribution and connectivity intervals in the network. How to effectively and reasonably evaluate their importance in the network is the key to optimizing the urban rail network structure and reducing operational risks. Taking the site as the research object and considering the topology, passenger volume, and passenger flow correlation of the urban rail network, the index of citation index is used to define node importance metric based on the h-index. Furthermore, the method of calculating the importance degree of urban rail transit network nodes based on h-index is proposed. The validity of the method is verified by the data of Beijing urban rail network in 2016, and the results are compared with the existing central index based on the network topology characteristics.

The flame retardant of epoxy resin (EP) with simultaneously eco-friendly environment and high efficiency had gained much attention. Here, a tactful route was utilized to chemically modify ammonium polyphosphate (Fe@APP) through ion exchange reaction between APP and Iron (III) nitrate hexahydrate. Adequate characterizations confirmed the successful preparation of Fe@APP, then the cone calorimeter results displayed that the heat and smoke release of 5 wt% Fe@APP/EP composite had been largely decreased. In addition, the flame-retardant mechanism investigation illustrated that iron (III) not only obviously efficiently catalyzed the charring process of EP, but also promoted the early formation of P–O–C group in Fe@APP/EP composite via catalysis during combustion, which could reinforce the intumescent char structure and fire safety for EP.

Terpene content of two distinct collections of the nudibranch Phyllidia coelestis from the South China Sea has been chemically analyzed. A series of amphilectene diterpenes, most likely of dietary origin, with isocyano and formamido functionalities have been isolated from both collections and spectroscopically characterized by an exhaustive nuclear magnetic resonance (NMR) analysis. Interestingly, the structural architecture of compounds 5&ndash, 7 and 9 with both 8,13-cis and 12,13-cis ring junctions is unprecedented in the amphilectene skeleton. Metabolite 3, which was the most abundant in the nudibranch&rsquo, s mantle, has been shown to deter feeding by a generalist predator, supporting its involvement in chemical defense.

A detailed chemical investigation of the nudibranch Glossodoris atromarginata collected from Weizhou Island, South China Sea, yielded a new spongian-type diterpene 1, together with the four known-related compounds 2-5. The structure of the new compound 1 was elucidated by the detailed spectroscopic analysis, the comparison of the spectroscopic data with the known diterpene isoagatholactone, and the 13 C chemical shift calculation. In addition, evidence for the absolute stereochemistry of the known compound 2 was, for the first time, provided by the application of time-dependent density functional theory electronic circular dichroism calculation.

With the emergence of all kinds of location services applications, massive location data are collected in real time. A hierarchical fast density clustering algorithm, DBSCAN(density based spatial clustering of applications with noise) algorithm based on Gauss mixture model, is proposed to detect clusters and noises of arbitrary shape in location data. First, the gaussian mixture model is used to fit the probability distribution of the dataset to determine different density levels; then, based on the DBSCAN algorithm, the subdatasets with different density levels are locally clustered, and at the same time, the appropriate seeds are selected to complete the cluster expansion; finally, the subdatasets clustering results are merged. The method validates the clustering effect of the proposed algorithm in terms of clustering accuracy, different noise intensity and time efficiency on the test data of public data sets. The experimental results show that the clustering effect of the proposed algorithm is better than traditional DBSCAN. In addition, the passenger flow data of the night peak period of the actual site is used to identify the uneven distribution of passengers in the station. The result of passenger cluster identification is beneficial to the optimization of service facilities, passenger organization and guidance, abnormal passenger flow evacuation.

To improve the accuracy and efficiency of fault data identification of traffic detectors is crucial in order to decrease the probability of unexpected failures of the intelligent transportation system (ITS). Since convolutional fault data recognition based on traffic flow three-parameter law has a poor capability for multiscale of fault data, PCA (principal component analysis) is adopted for traffic fault data identification. This paper proposes the fault data detection models based on the PCA model, MSPCA (multiscale principal component analysis) model and improved MSPCA model, respectively. In order to improve the recognition rate of traffic detectors&rsquo, fault data, the improved MSPCA model combines the wavelet packet energy analysis and PCA to achieve traffic detector data fault identification. On the basis of traditional MSPCA, wavelet packet multi-scale decomposition is used to get detailed information, and principal component analysis models are established on different scale matrices, and fault data are separated by wavelet packet energy difference. Through case analysis, the feasibility verification of traffic flow data identification method is carried out. The results show that the improved method proposed in this paper is effective for identifying traffic fault data.

A novel diterpenoid, sarcomililate A (1), possessing a previously undescribed tricyclo[11.3.0.0

Recognizing and diagnosing the state of traffic flow is an important research area, which is the basis of improving the level of traffic management and the quality of traffic information services. However, due to the increasing amount of traffic data collected, the traffic management system is facing the problem of “information surplus”. After finishing several process, including data preprocessing, attribute reduction and rule acquisition, finally obtained the knowledge rules of the traffic flow’s state. Using the method of knowledge discovery can reveal some hidden, unknown and valuable information from the huge amount of traffic flow information, so as to provide rules and decision-making basis for traffic management department.

Practical aspects of the oligopeptide AAKLVFF as an alignment medium are discussed, including large-scale synthesis of the oligopeptide, detailed description of preparation of the alignment medium, and acquisition of the RDCs. The resulting orienting medium is stable and highly homogeneous with tunable alignment strength in methanol.Practical aspects of the oligopeptide AAKLVFF as an alignment medium are discussed, including large-scale synthesis of the oligopeptide, detailed description of preparation of the alignment medium, and acquisition of the RDCs. The resulting orienting medium is stable and highly homogeneous with tunable alignment strength in methanol. We demonstrated its applicability on the stereochemical elucidation of two natural products, oridonin and α-santonin.

The quality control and evaluation of traffic detector data are a prerequisite for subsequent applications. Considering that the PCA method is not ideal when detecting fault information with time-varying and multi-scale features, an improved MSPCA model is proposed in this paper. In combination with wavelet packet energy analysis and principal component analysis, data fault identification for traffic detectors is realized. On the basis of traditional multi-scale principal component analysis, detailed information is obtained by wavelet packet multi-scale decomposition, and a principal component analysis model is established in different scale matrices; fault data is separated by wavelet packet energy difference; according to the time characteristics and space of the detector data Correlation fixes fault data. Through case analysis, the feasibility of the method was verified.

The node importance measurement plays an important role in analyzing the reliability of the urban road network. In this thesis, the topological structure, geographic information and traffic flow characteristics of urban road network are all considered, and methods of node importance measurement of urban road network are proposed based on a spatially weighted degree model and h-index from different perspectives. Experiments are given to show the efficiency and practicability of the proposed methods.

In order to improve the printing quality, 10 kinds of different manufacturers, different gram weight offset paper are used in this title, measure the quantitative, thickness, gloss, K&N values, and roughness of those paper. Print papers through IGTAIC2-5, calculate the ink transfer rate and transfer parameters. Using SPSS to analysis relationship between the performance of paper and ink transfer rate, transfer parameter b, k, f ' by using mathematical methods. The research results show that the performance of the paper affect ink transfer rate and transfer parameter b, k, f '; The tightness, roughness ,glossiness and K&N value have notable effect on the ink transfer rate and transfer parameter k; transfer parameter b, f ' has no remarkable influence.