1. Nuclear Transfer-Derived Epiblast Stem Cells Are Transcriptionally and Epigenetically Distinguishable from Their Fertilized-Derived Counterparts
- Author
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Julien Maruotti, Roger A. Pedersen, Jean-Paul Renard, I. Gabrielle M. Brons, Qi Zhou, Vincent Brochard, Alice Jouneau, Jun Liu, Amélie Bonnet-Garnier, Hélène Jammes, Luc Jouneau, Ludovic Vallier, Xiang Peng Dai, Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Sino-French Laboratory LABIOCEM, Institute of Zoology, Chinese Academy of Sciences [Beijing], Chinese Academy of Sciences (CAS), State Key Laboratory of Reproductive Biology, Institute of Genetics and Developmental Biology, CAS, Partenaires INRAE, and University of Cambridge [UK] (CAM)
- Subjects
Nuclear Transfer Techniques ,animal structures ,PLURIPOTENT STEM CELLS ,[SDV]Life Sciences [q-bio] ,Fertilization in Vitro ,Embryoid body ,Biology ,REPROGRAMMING ,Cell Line ,Epigenesis, Genetic ,CLONING ,Mice ,03 medical and health sciences ,0302 clinical medicine ,NUCLEAR TRANSFER ,Animals ,Inner cell mass ,[INFO]Computer Science [cs] ,Induced pluripotent stem cell ,Cell Shape ,Cell Proliferation ,030304 developmental biology ,0303 health sciences ,Gene Expression Profiling ,Stem Cells ,Cell Biology ,Molecular biology ,Embryonic stem cell ,GENOMIQUE ,Mice, Inbred C57BL ,Epiblast ,embryonic structures ,Molecular Medicine ,Stem cell ,Genomic imprinting ,Reprogramming ,Biomarkers ,Germ Layers ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Mouse embryonic pluripotent stem cells can be obtained from the inner cell mass at the blastocyst stage (embryonic stem cells, ESCs) or from the late epiblast of postimplantation embryos (epiblast stem cells, EpiSCs). During normal development, the transition between these two stages is marked by major epigenetic and transcriptional changes including DNA de novo methylation. These modifications represent an epigenetic mark conserved in ESCs and EpiSCs. Pluripotent ESCs derived from blastocysts generated by nuclear transfer (NT) have been shown to be correctly reprogrammed. However, NT embryos frequently undergo abnormal development. In the present study, we have examined whether pluripotent cells could be derived from the epiblast of postimplantation NT embryos and whether the reprogramming process would affect the epigenetic changes occurring at this stage, which could explain abnormal development of NT embryos. We showed that EpiSCs could be derived with the same efficiency from NT embryos and from their fertilized counterparts. However, gene expression profile analyses showed divergence between fertilized- and nuclear transfer-EpiSCs with a surprising bias in the distribution of the differentially expressed genes, 30% of them being localized on chromosome 11. A majority of these genes were downregulated in NT-EpiSCs and imprinted genes represented a significant fraction of them. Notably, analysis of the epigenetic status of a downregulated imprinted gene in NT-EpiSCs revealed complete methylation of the two alleles. Therefore, EpiSCs derived from NT embryos appear to be incorrectly reprogrammed, indicating that abnormal epigenetic marks are imposed on cells in NT embryos during the transition from early to late epiblast.
- Published
- 2010
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