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To investigate the diagnostic value of quantitative susceptibility mapping (QSM) in mild cognitive impairment (MCI) of aluminium (Al) workers.The basic data of 53 workers in an Al factory were collected and divided into the MCI group and normal control (NC) group by Montreal Cognitive Assessment (MoCA) scores. All participants were tested for plasma Al concentration and had magnetic resonance imaging (MRI). The QSM values of many areas of the brain were delineated and measured. Independent two-sample t-tests or non-parametric tests were used to compare the parameter values between the two groups. Spearman's correlation analysis was performed between QSM values, MoCA scores, and plasma Al concentration. The receiver operating characteristic curve and z test were performed to assess diagnostic efficacy and the best parameter.There was no difference in age and educational level. Plasma Al concentration of the MCI group was higher than that of NC group (p=0.057). QSM values of the left hippocampus, left dentate nucleus, right substantia nigra, and left putamen in MCI group were higher than that of NC group (p0.05), and the left hippocampus had the best diagnostic efficacy. QSM values correlated negatively with MoCA scores. No correlation was found between QSM values and plasma Al concentration (p0.05).QSM might be a neuroimaging marker for the diagnosis of MCI. The left hippocampus showed the best diagnostic efficacy. Plasma Al concentration of the MCI group was higher than that of the NC group. A correlation between QSM and plasma Al concentration was not found.

SARS-CoV-2 has infected more than 600 million people worldwide and caused more than 6 million deaths. The emerging novel variants have made the epidemic rebound in many places. Meteorological factors can affect the epidemic spread by changing virus activity, transmission dynamic parameters and host susceptibility. This paper systematically analyzed the currently available laboratory and epidemiological studies on the association between the meteorological factors and COVID-19 incidence, in order to provide scientific evidence for future epidemic control and prevention, as well as developing early warning system.新型冠状病毒已在全球范围内感染超过6亿人，并造成超过600万人死亡，不断出现的新型变异株使得新型冠状病毒肺炎疫情态势在多地出现反弹。气象因素可通过改变病毒活性、传播动力学条件和宿主易感性等，影响疫情传播，本文通过系统梳理和分析气象因素对疫情影响的实验室和流行病学研究结果，分析了气象因素与疾病传播之间的关联，以期为未来疫情防控及开发早期预警系统提供科学参考。.

This study aimed to investigate the value of combining the detection of serum tumor markers, namely, carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 199, CA 242, and CA 50, with fecal occult blood (FOB) testing in the diagnosis of colorectal cancer (CRC). One hundred patients with CRC who were diagnosed and treated at the First Hospital of Jiaxing, Zhejiang Province, China, between January 2019 and April 2020 were enrolled as the case group, and 200 healthy people who underwent a physical examination at the hospital during the same period were recruited as the control group. The concentrations of CEA, CA199, CA242, and CA50 in serum were measured alongside FOB indicators. Compared with the control group, the concentrations of CEA, CA199, CA242, and CA50 in the case group were significantly higher, and they were related to age, tumor differentiation, tumor stage, and other clinicopathological features (P0.05). The diagnostic performance of the combination of four tumor markers for CRC was significantly better than when using a single marker (four-combined test: AUC (area under the curve) AUC=0.80), and the diagnostic performance was further improved after adding the fecal occult blood test test (FOBT) results (five-combined test: AUC=0.90). The combined detection of the five indexes was found to be effective for the early diagnosis of CRC (AUC=0.87). We concluded that the detection of serum tumor markers CEA, CA199, CA242, and CA50 combined with an FOBT could significantly improve the sensitivity and accuracy of a CRC diagnosis and contributed to an early diagnosis and appropriate treatment.

Stitching skin wounds is one of the essential skills of a surgeon. Whether it is a traumatic wound or a surgical incision, choosing the most appropriate closure technique according to its characteristics is an important factor for good healing. Various skin wounds suturing techniques have been created and improved over the years, which have advantages of simple operation, precise alignment, reducing tension of the wound edges, and reducing scar formation, etc. Although these techniques provide more options for wound suture, they also put forward requirements for the judgment and operation ability of the operators. This article summarizes the advantages and disadvantages of the different skin wounds suturing techniques and their clinical application.皮肤伤口缝合是外科医师必备技能之一。无论是外伤伤口还是手术切口，根据其特点选择合适的缝合方式是愈合良好的重要因素。多年来，各种皮肤伤口缝合技术不断地涌现，经不断改进后形成了操作简便、对合精准、降低创缘张力及减少瘢痕形成等优势。这些技术虽然为伤口的缝合提供了更多选择，但是也对术者的判断能力和操作能力提出了要求。本文就不同皮肤伤口缝合技术的优点和不足及其临床应用进行综述。.

Monogenic nephrolithiasis is comparatively rare in clinical practice, but its yearly increasing incidence and impacts on physical and mental health are worth wide attention. At present, management of monogenic nephrolithiasis are still the medication and surgery. This article regards the research progress on primary hyperoxaluria as the breakthrough point to review the etiological therapy of monogenic nephrolithiasis. We aim to promote more studies on other monogenic nephrolithiasis, more methods and drugs for gene therapy, precise and individualized treatment of monogenic nephrolithiasis, and thus to promote the prevention and treatment of monogenic nephrolithiasis in China.单基因肾结石病虽在临床较为少见，但其逐年增加的发病率及对患者身心健康的影响足以引起重视。目前单基因肾结石病的治疗尚停留于药物及手术层面，本文以原发性高草酸尿症基因治疗的进展为切入点，对单基因肾结石病在病因学层面的治疗进行论述，以期推动更多基因治疗层面的研究和尝试，推动基因治疗方式和药物的研发与探索，推动单基因肾结石病精准化及个体的治疗模式的完善，进而提升该病的诊疗水平。.

亚临床甲状腺功能减退症（甲减）是最常见的甲状腺功能异常，血清促甲状腺激素（TSH）是诊断亚临床甲减的关键指标。TSH参考范围不统一是既往亚临床甲减患病率差异较大的主要原因。目前推荐采用美国国家临床生物化学学会（NACB）标准选择参考人群，用经对数转换后的2.5%~97.5%可信区间确定TSH参考范围。但是，我们还应充分考虑个体内和个体间因素对TSH参考范围的影响，在试剂盒参考范围的基础上，结合上述因素，分析患者血清TSH升高的原因，才能对亚临床甲减做出合理的诊断和干预。.

Frequent urban flooding disasters can cause severe economic and property losses. Accordingly, the construction of sponge city has become critical to alleviating urban flooding. However, the functional and structural integration of Green Infrastructure (GI) and Gray Drainage Facility (GDF) is still a matter of concern. This study proposed a novel implementation framework for GI and GDF synchronization optimization (G-GSOIF) based on the SWMM and SUSTAIN models, and used data from Beilin District in Xi'an, China to verify the effects. The results show that the spatiotemporal integrated optimization design of GI and GDF proves to be effective in stormwater management. The total investment was reduced by 16.7% and economic benefit was increased by 15.4% based on disaster risk control, and the utilization rate of rainwater resources exceeded 40%. The Staged optimization model (SSOM) based on the SUSTAIN model established in the G-GSOIF was demonstrated to effectively cope with the impact of future climate change by adjusting and optimizing the design scheme dynamically in different simulation scenarios. Integrated LID (I-LID) measures are conducive for simulation of large catchment areas, and have the same implementation effect as distributed LID measures. The results of this study could support decision-making for urban stormwater management and sponge city construction.

患儿 男，9岁7月龄，因“反复感染3年余，阵发性头痛3个月”就诊，临床表现为EB病毒感染后继发噬血细胞性淋巴组织增生症，反复肺炎，右侧额颞部头痛和头晕，发作性左下肢无力。精细分型提示成熟B细胞水平降低，自然杀伤细胞明显减少，免疫球蛋白水平降低。基因检测提示SH2D1A基因 c.163CT（p.R55X）杂合变异。脑脊液中EB病毒DNA阳性，头颅磁共振成像提示脑白质损伤，头颅磁共振成像血管造影提示左前脑动脉有局部结节。诊断为X连锁淋巴细胞增殖性疾病1型，经干细胞移植该患儿脑血管炎、颅内EB病毒感染症状以及免疫功能明显好转。.

A multiple tuned mass damper (MTMD) system is presented for vibration control of a cable-supported roof structure of a large exhibition center. The MTMD system consists of three TMD sub-systems, which are tuned to each of three dominating vertical modes of the structure under wind excitation. Each TMD sub-system is composed of multiple TMDs with small masses spread at multiple locations with large response in the corresponding mode. Several key points in the design of MTMD scheme are discussed including determinations of dominating modes and damping ratios of TMDs as well as MTMD performance based on wind vibration responses. This MTMD system is going to be installed on a real structure in the near future.

We have performed kinematically complete experiments on single-electron capture in slow ${\mathrm{He}}^{2+}$-Ar collisions. Besides the pure capture to the ${\mathrm{He}}^{+}(n=2)$ level, capture into the deep ${\mathrm{He}}^{+}(n=1)$ state with simultaneous excitation of another target electron is also observed. In contrast to the pure capture, the total cross section for this two-electron transition decreases with increasing collision energy, and its angular-differential cross section exhibits a much slighter slope. We take these observations as evidence for electron capture mediated by electron-electron interactions.

The Eurotiales is a relatively large order of Ascomycetes with members frequently having positive and negative impact on human activities. Species within this order gain attention from various research fields such as food, indoor and medical mycology and biotechnology. In this article we give an overview of families and genera present in the Eurotiales and introduce an updated subgeneric, sectional and series classification for Aspergillus and Penicillium. Finally, a comprehensive list of accepted species in the Eurotiales is given. The classification of the Eurotiales at family and genus level is traditionally based on phenotypic characters, and this classification has since been challenged using sequence-based approaches. Here, we re-evaluated the relationships between families and genera of the Eurotiales using a nine-gene sequence dataset. Based on this analysis, the new family Penicillaginaceae is introduced and four known families are accepted: Aspergillaceae, Elaphomycetaceae, Thermoascaceae and Trichocomaceae. The Eurotiales includes 28 genera: 15 genera are accommodated in the Aspergillaceae (Aspergillago, Aspergillus, Evansstolkia, Hamigera, Leiothecium, Monascus, Penicilliopsis, Penicillium, Phialomyces, Pseudohamigera, Pseudopenicillium, Sclerocleista, Warcupiella, Xerochrysium and Xeromyces), eight in the Trichocomaceae (Acidotalaromyces, Ascospirella, Dendrosphaera, Rasamsonia, Sagenomella, Talaromyces, Thermomyces, Trichocoma), two in the Thermoascaceae (Paecilomyces, Thermoascus) and one in the Penicillaginaceae (Penicillago). The classification of the Elaphomycetaceae was not part of this study, but according to literature two genera are present in this family (Elaphomyces and Pseudotulostoma). The use of an infrageneric classification system has a long tradition in Aspergillus and Penicillium. Most recent taxonomic studies focused on the sectional level, resulting in a well-established sectional classification in these genera. In contrast, a series classification in Aspergillus and Penicillium is often outdated or lacking, but is still relevant, e.g., the allocation of a species to a series can be highly predictive in what functional characters the species might have and might be useful when using a phenotype-based identification. The majority of the series in Aspergillus and Penicillium are invalidly described and here we introduce a new series classification. Using a phylogenetic approach, often supported by phenotypic, physiologic and/or extrolite data, Aspergillus is subdivided in six subgenera, 27 sections (five new) and 75 series (73 new, one new combination), and Penicillium in two subgenera, 32 sections (seven new) and 89 series (57 new, six new combinations). Correct identification of species belonging to the Eurotiales is difficult, but crucial, as the species name is the linking pin to information. Lists of accepted species are a helpful aid for researchers to obtain a correct identification using the current taxonomic schemes. In the most recent list from 2014, 339 Aspergillus, 354 Penicillium and 88 Talaromyces species were accepted. These numbers increased significantly, and the current list includes 446 Aspergillus (32 % increase), 483 Penicillium (36 % increase) and 171 Talaromyces (94 % increase) species, showing the large diversity and high interest in these genera. We expanded this list with all genera and species belonging to the Eurotiales (except those belonging to Elaphomycetaceae). The list includes 1 187 species, distributed over 27 genera, and contains MycoBank numbers, collection numbers of type and ex-type cultures, subgenus, section and series classification data, information on the mode of reproduction, and GenBank accession numbers of ITS, beta-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) gene sequences.

原发性免疫缺陷病（PID）多在婴幼儿时期发病。PID与预防接种的问题主要有3方面。早期识别和诊断PID是重要前提；PID患儿可接种灭活疫苗，少数PID需经免疫专科医生评估谨慎决定是否接种减毒活疫苗；当患儿出现减毒活疫苗株感染时，应免疫评估排除存在PID的可能。.

The composites of NiCo

The aim of this study was to clarify the role of long non-coding RNA (lncRNA) HOXA-AS2 in influencing the proliferative, migratory and apoptotic abilities of human aortic vascular smooth muscle cells (HA-VSMCs) by absorbing microRNA-877-3p (miRNA-877-3p).HOXA-AS2 level in HA-VSMCs treated with different doses of oxidized low-density lipoprotein (ox-LDL) and for different time points was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). After transfection of si-HOXA-AS2 in HA-VSMCs undergoing ox-LDL treatment, the viability, apoptotic rate and migration of cells were detected, respectively. Meanwhile, the subcellular distribution of HOXA-AS2 was analyzed. The Dual-Luciferase reporter gene assay was applied to verify the binding relationship between HOXA-AS2 and miRNA-877-3p. MiRNA-877-3p level in HA-VSMCs treated with different doses of ox-LDL was determined as well. Furthermore, the regulatory effects of HOXA-AS2/miRNA-877-3p axis on cellular behaviors of HA-VSMCs were determined.HOXA-AS2 expression was upregulated by ox-LDL treatment in a time- and dose-dependent manner. After being treated with 100 mg/L ox-LDL for 48 h, the proliferative and migratory abilities of HA-VSMCs were significantly enhanced, while apoptosis was inhibited. Conversely, these changes were reversed by transfection of si-HOXA-AS2. HOXA-AS2 was mainly distributed in the nuclear fraction. Dual-Luciferase reporter gene assay confirmed the direct binding relationship between HOXA-AS2 and miRNA-877-3p. Moreover, miRNA-877-3p was markedly downregulated after transfection of si-HOXA-AS2. MiRNA-877-3p expression decreased gradually with an increased dose of ox-LDL. In addition, knockdown of miRNA-877-3p could reverse the regulatory effects of HOXA-AS2 on proliferative, migratory and apoptotic abilities of HA-VSMCs.HOXA-AS2 is upregulated after HA-VSMCs injury, which accelerates the proliferative and migratory abilities, and inhibits the apoptosis of vascular smooth muscle cells by absorbing miRNA-877-3p.

Ovarian cancer (OC) is one of the most lethal gynecologic malignant tumors. Emerging evidence has indicated that the dysregulation of microRNAs (miRNAs/miRs) participates in the OC progression. It has been revealed that miR-149 acts either as an oncogene or a tumour suppressor in various human tumors. The current study focused on the biological roles and potential mechanism of miR-149 in OC.Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine the level of miR-149 expression in 72 pairs of OC tissues and para-cancerous specimens. We further measured the miR-149 levels in OC cells. As we indicated that miR-149 inhibited OC cell viability, we further explored the roles of miR-149 in OC cell invasion and migration by performing the transwell assays. As we suggested that MSI2 was one target for miR-149 in OC cell lines, the expressions and clinical significance of MSI2 in OC were further investigated.We first detected miR-149 expressions in the OC tissues using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and the data showed that miR-149 was dramatically downregulated in the OC tissue samples in comparison to matched normal tissue samples. Additionally, the downregulation of miR-149 in OC was found to be related to the poor prognosis and malignant clinicopathologic characteristics of patients with OC. MiR-149 overexpression significantly suppressed the OC cell proliferation, invasion, and migration as determined by functional assays, including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays. Furthermore, the dual-luciferase reporter assay demonstrated that MSI2 was an efficient target of miR-149 in OC cells. Finally, some findings also revealed that miR-149 exerted its biological function in OC cells via direct regulation of phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT).Collectively, miR-149 exerted anti-OC roles at least partially by regulating MSI2 via PI3K/AKT. The findings of this study suggested that miR-149 might be a promising target in the diagnosis and prognosis for OC patients.

The Lop Nur region, as the junction of the Silk Roads, played an important role in ancient human migration between the East and the West, especially during the Han-Jin Dynasties (second century CE to fourth century CE). However, the scale and volume of human mobility in the region remain poorly understood. Here, we present a multi-approach (wood identification, strontium and oxygen isotopes of tooth enamel and historical documents) to investigate the extent of human mobility in the Lop Nur region. From a Han-Jin Dynasties cemetery, the appearance of nonlocal wood might suggest the existence of human migratory behaviors in the Lop Nur region. Furthermore, a piece of lacquerware with the possible origin of eastern China and a large-scale mural tomb likely belonged to a Kushan emigrant indicate the existence of long-distance connections across the Asia interior. Strontium and oxygen isotope data show a highly mobile population in the Lop Nur region, and the great isotopic variation suggests that these immigrants have diverse origins. In context with historical documents, we infer that the Lop Nur region was once the political, economic, and cultural meeting place of various societies, and also a communication corridor on the Silk Roads during the Han-Jin Dynasties.

Germanium selenide is a promising material for electronic, photovoltaic, and thermoelectric applications; however, structural phase transitions of GeSe under pressure are controversial. Combining evolutionary algorithms, density functional theory, tight-binding method, and laser-heated diamond anvil cell experiments, pressure-induced phase transitions of GeSe are thoroughly investigated. Two novel intermediate phases are predicted to exist in between the well-known α-GeSe and the recently discovered β-GeSe under high pressure. α-GeSe is found to transform into a rhombohedral crystal structure with a space group of R3m at a low hydrostatic pressure. The R3m phase of GeSe exhibits robust ferroelectricity analogous to GeTe. By further increasing the pressure to approximately 6 GPa, the R3m phase is predicted to transform into a rock-salt structure, becoming a 3D topological crystalline insulator with an inverted band structure. The newly discovered GeSe high-pressure phases greatly enrich our knowledge of IV–VI compounds. The atoms in germanium selenide can adopt more configurations than previously thought according to a study by researchers in China. A phase change, the rearrangement of atoms in a solid from one crystal structure to another, can radically alter a material’s properties. Such a change in phase can be induced by exposing matter to high pressure. Kuo Li from the Center for High Pressure Science and Technology Advanced Research in Beijing, Yue Chen from The University of Hong Kong, and their co-workers have combined multiple computational and experimental techniques to identify the phases of germanium selenide, a material useful for energy conversion. Recently a high-pressure phase called β-GeSe was discovered in addition to the common low-pressure α-GeSe phase. The researchers predict two further phases at intermediate pressure, each with its own distinctive electrical properties. Pressure-induced phase transitions in GeSe are investigated from theory and experiment. Two new phases are predicted to exist in-between the well-known α-GeSe and the recently discovered β-GeSe under pressures. It is predicted that α-GeSe transforms into a rhombohedral structure with a space group of R3m and exhibits robust ferroelectricity at a low hydrostatic pressure. Laser-heated DAC experiments have been conducted to provide further evidence on the existence of the R3m phase. By increasing the hydrostatic pressure to approximately 6 GPa, the R3m phase is predicted to transform into a rock-salt crystal structure ( $$Fm\bar 3m$$ ) and become a topological crystalline insulator.

In Southern China, plants are usually exposed to cold stress during winter in an unheated greenhouse, but due to the high energy consumption and costs, most of the greenhouses remain unheated. In an attempt to find a simple and affordable solution to this problem, this study was undertaken. In this research, Capsicum frutescens L. plants were studied to investigate the effect of different root zone temperatures on its growth and chlorophyll fluorescence characteristics under cold stress. The plants were cultivated under cold stress conditions in a root zone temperature (RZT) control system where the roots were subjected to four different root-zone temperature treatments of 20°C-T20, 25°C-T25, 45°C-T45 and a control CK group. Growth characteristics studied included plant height, stem diameter, plant width, root length, biomass accumulation. Whilst fluorescence characteristics investigated were chlorophyll fluorescence ratio Fv/Fm, photochemical quenching (qL), efficiency of Photosystem II (Y[II]) and electron transport rate (ETR). Chlorophyll content in the leaves of the plants was also investigated. The findings demonstrated that plants in the CK group suffered a detrimental effect on the growth characteristics registering the lowest values in the measured variables. Conversely, the highest values were observed in T25 RZT treatment. In fluorescence characteristics, values of Fv/Fm were maintained at between 0.8 and 0.83 but also suffered a photo-inhibitory depression in CK and T45 RZT treatments to Fv/Fm values of

As the junction of the Silk Roads, the Tarim Basin played a critical role in human migration and cultural interaction between the East and the West. Recently, population migration patterns have been increasingly reconstructed from strontium isotope ratios ( 87 Sr/ 86 Sr) in archaeological human skeletons, and further constraints on their provenance require a bioavailable 87 Sr/ 86 Sr map across the basin. Based on measurement data from 44 river samples in this study and 29 samples from published data, we generate the first map of bioavailable 87 Sr/ 86 Sr values across the Tarim Basin and the neighbouring area. From the baseline map, three geologically distinct regions can be identified: (1) the Yili Block, Tian Shan orogenic belt and northern Tarim Block with lower ratios (0.7105 ± 0.0007 (1σ), n = 25); (2) the southern Tarim Block with higher ratios (0.7118 ± 0.0008, n = 29); and (3) the eastern Pamir Block with diverse values (0.7110 ± 0.0009, n = 19). This map is a promising tool for recognizing the origins of immigrants in the Tarim Basin and may aid understanding of the migration patterns and cultural communication along the Silk Roads.

Lack of normal differentiation was considered as a common defect in cancer cells. MS4A12, a colon-specific gene, belongs to MS4A family that plays an important role in differentiation, proliferation and cell cycle regulation. The aim of the study was to investigate MS4A12 role in colon cancer cell differentiation and its prognostic value in colon cancer. We used sodium butyrate (BS) to set up differentiated model of colon cancer cell line LoVo. Cell differentiation was evaluated with ALP activity and E-cadherin. We used BS (4 mmol/L) inducing differentiation of LoVo cell and found after BS treated over 48h MS4A12 variant-1 (one of MS4A12 gene transcripts) as well as ALP and E-cadherin of LoVo cells were all increased significantly. When silence MS4A12 variant-1, the elevation of ALP and E-cadherin in BS-treated cells were all inhibited. Besides, after silence MS4A12 variant-1, the cells showed significant resistances to BS function of induction cell cycle arrest and apoptosis. Survival analysis used GEO datasets GSE39582 and GSE38832 that include 681 distinct colon cancer samples. Log-rank test and Cox's proportional hazards regression were applied to analyzing single and multiple prognostic variables, respectively. In early stage colon cancer, the patients with low MS4A12 expression had a poor survival (HR=1.72; p=0.036), while in advanced stage colon cancer MS412 had little prognostic value (HR=0.89; p=0.601). These results indicated MS4A12 might relate to colon cancer cell differentiation and supposed to be a risk classification marker for early stage colon cancer.

MiR-199 expression is associated with liver cancer. Bioinformatics analysis revealed that miR-199 has a complementary binding site to the 3'-UTR region of Snail mRNA. This study investigated whether miR-199 plays a role in regulating Snail expression and affecting epithelial-mesenchymal transition (EMT) and invasion of hepatoma cells.The Dual-Luciferase reporter gene assay validated the targeted regulation between miR-199 and Snail. QRT-PCR was used to detect and compare the expression of miR-199 and Snail mRNA in human normal liver HL7702 cells, low metastatic MHCC97L cells, and high metastatic MHCC97H cells. MHCC97H cells were cultured in vitro and divided into two groups: miR-NC group and the miR-199 mimic group followed by the analysis of the expression of Snail, E-cadherin, and N-cadherin, as well as cell invasion ability by transwell assay.There was a targeted regulatory relationship between miR-199 and Snail mRNA. Compared with HL7702 cells, miR-199 expression was significantly decreased, and Snail expression was significantly increased in MHCC97L and MHCC97H cells, with more changes being observed in high metastatic MHCC97H cells. The transfection of miR-199 mimic significantly downregulated the expression of Snail and N-cadherin in MHCC97H cells, increased E-cadherin expression, inhibited the cell's EMT process, and invasion.The decrease of miR-199 expression plays a role in upregulating the expression of Snail and promoting EMT and invasion of hepatocarcinoma cells. The increase of the expression of miR-199 can inhibit the expression of Snail and inhibit the EMT process and invasion ability of hepatoma cells.

The aim of this study was to investigate the effects of retinoid X receptor α (RXRα) on the proliferation and apoptosis of pancreatic cancer cells through the transforming growth factor-β (TGF-β)/Smad signaling pathway.The expression of RXRα in pancreatic cancer tissues and para-carcinoma tissues was detected via immunohistochemistry. Human pancreatic cancer PANC-1 cells were cultured and treated with RXRα in vitro. The apoptosis rate of cells was detected via flow cytometry. Furthermore, changes in the protein expression level of TGF-β/Smad signaling pathway were detected via Western blotting.The protein expression level of RXRα in pancreatic cancer tissues was significantly higher than that of para-carcinoma tissues. RXRα significantly promoted the proliferation and inhibited the apoptosis of pancreatic cancer cells. Moreover, RXRα could also activate the TGF-β/Smad signaling pathway.RXRα promotes the proliferation and inhibits the apoptosis of pancreatic cancer cells through the TGF-β/Smad signaling pathway.

Rabies is one of the major public health problems in China, and the mortality rate of rabies remains the highest among all notifiable infectious diseases. A meta-analysis was conducted to investigate the post-exposure prophylaxis (PEP) vaccination rate and risk factors for human rabies in mainland China. The PubMed, Web of Science, Chinese National Knowledge Infrastructure, Chinese Science and Technology Periodical and Wanfang databases were searched for articles on rabies vaccination status (published between 2007 and 2017). In total, 10 174 human rabies cases from 136 studies were included in this meta-analysis. Approximately 97.2% (95% confidence interval (CI) 95.1–98.7%) of rabies cases occurred in rural areas and 72.6% (95% CI 70.0–75.1%) occurred in farmers. Overall, the vaccination rate in the reported human rabies cases was 15.4% (95% CI 13.7–17.4%). However, among vaccinated individuals, 85.5% (95% CI 79.8%–83.4%) did not complete the vaccination regimen. In a subgroup analysis, the PEP vaccination rate in the eastern region (18.8%, 95% CI 15.9–22.1%) was higher than that in the western region (13.3%, 95% CI 11.1–15.8%) and this rate decreased after 2007. Approximately 68.9% (95% CI 63.6–73.8%) of rabies cases experienced category-III exposures, but their PEP vaccination rate was 27.0% (95% CI 14.4–44.9%) and only 6.1% (95% CI 4.4–8.4%) received rabies immunoglobulin. Together, these results suggested that the PEP vaccination rate among human rabies cases was low in mainland China. Therefore, standardised treatment and vaccination programs of dog bites need to be further strengthened, particularly in rural areas.

The flue gas emission of the building ceramics industry accounts for 85% of the whole ceramic industry in China, and it is the critical sector of air pollution control in the ceramic industry. The advanced available techniques (AAT) of air pollution prevention and control are of considerable significance to promote the upgrade of pollution prevention measures and technological progress. The paper studied and established the method of screening and evaluating the AAT for the prevention and control of air pollution in the building ceramics industry. Through collection and analysis of three normal pollutant emission concentrations of ten available techniques (AT), to select the AAT that meet at least one pollutant of all the plants was lower than 50% of the national standard emission concentration threshold in China. Based on the experience of the European Union and the comprehensive evaluation of industry associations, research institutions, and pollution control experts, five AAT were selected. The findings will provide an essential reference for the building ceramics industry to select advanced techniques, and provide significant technical support for the revision of the ceramic industrial pollutant emission standard for and selection of pollution prevention and control techniques (PPT and PCT) for pollutant discharge permission management.

The damage mode and failure mechanism of the PBO composite are studied of the penetrating speed from 900 m/s to 1900 m/s, based on the dynamic response of the PBO fiber-reinforced composite target under high-speed penetration. Firstly, the mechanical properties of the projectile and the target material under high strain rate were characterized, and then the penetration of high-speed projectile into target is simulated. Finally, the penetration test is verified. The results show that the dynamic response mechanism of PBO composite target under high-speed penetration varies significantly with the penetrating speed.

目的 观察输注纯化供者CD34+细胞治疗单倍型造血干细胞移植后移植物功能不良的疗效和安全性。 方法 对2014年1月至2018年3月期间接受供者纯化CD34+细胞治疗的12例单倍型造血干细胞移植后移植物功能不良患者进行回顾性分析。 结果 12例患者中男9例、女3例，中位年龄26（14~54）岁；急性淋巴细胞白血病4例，急性髓系白血病5例，慢性髓性白血病2例，慢性粒-单核细胞白血病1例。分选产物CD34+细胞纯度为92.0%（44.0%~97.0%），回收率为55.0%（45.0%~96.7%），回输CD34+细胞的中位数为1.9（0.9~4.4）×106/kg，CD3+细胞中位数为0.6（0.3~2.0）×104/kg。中性粒细胞恢复中位时间为18（14~39）d，血小板恢复中位时间为29（16~153）d，红细胞恢复中位时间为60（9~124）d。12例患者输注过程中未发生严重不良反应，10例获得完全缓解，1例患者获得部分缓解，1例患者无效，未发生重症感染和重度GVHD。 结论 回输供者纯化CD34+细胞是单倍型造血干细胞移植后植入功能不良的一种安全、有效治疗方法。