547 results on '"Wurm, Florian M."'
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2. Alpha1-antitrypsin improves survival in murine abdominal sepsis model by decreasing inflammation and sequestration of free heme
3. Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
4. Effects of Methotrexate on Recombinant Sequences in Mammalian Cells
5. Fluid dynamics of flow fields in a disposable 600-mL orbitally shaken bioreactor
6. Analysis of volumetric mass transfer coefficient (kLa) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors
7. A new T‐antigen negative HEK293 cell line with improved AAV productivity
8. Human PBMCs Form Lipid Droplets in Response to Spike Proteins.
9. A simple plasmid-based transient gene expression method using High Five cells
10. Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
11. Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line
12. Lentiviral Vectors for Rapid and Efficient Recombinant CHO Cell Line Generation
13. Recombinant Antibody Yield Over 2 g/L by Transient Transfection of HEK 293 EBNA Cells in a Fed-Batch Process
14. Engineering Principles and Cell Culture Performance of Orbitally Shaken Cylindrical Bioreactors
15. GFP-Expressing Bladder Fibroblasts for Applications in Tissue Engineering
16. Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells
17. A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells
18. Transgene mRNA Levels and Stability are Key Factors to Enhance Transient Gene Expression in CHO DG44 Cells
19. Cellular Proteins in Conditioned Medium Inhibit Polyethylenimine-Mediated Transfection of CHO Cells
20. Generation of High-Producing CHO Cell Lines by Piggybac Transposition
21. High Cell Density Transient Gene Expression in HEK 293 EBNA Cells
22. Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance
23. A new T-antigen negative HEK293 cell line with improved AAV productivity
24. Optimization and Comparison of Different DNA Methyl Transferase and Histone Deacetylase Inhibitors for Enhancing Transient Protein Expression
25. A Serum-Free, Transient Transfection System for Enhancing Production of Recombinant Antibodies in Mammalian Cells
26. Helical Tracks in Shaken Cylindrical Bioreactors Improve Oxygen Transfer and Increase Maximum Cell Density Obtainable for Suspension Cultures of Mammalian Cells
27. Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cells
28. Transient Gene Expression in Chinese Hamster Ovary Cells at Low Temperature – The Effects of Cold-Induced Proteins and an mRNA Regulatory Element
29. Virus-free transient protein production in Sf9 cells
30. 1000 Non-instrumented Bioreactors in a Week : Novel Disposable Technologies for Rapid Scale up of Suspension Cultures
31. Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1
32. Release of Plasmid-DNC from PEI/DNA Particles
33. Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
34. DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine
35. Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension
36. Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity
37. Sodium Butyrate Treatment: A Study of Critical Parameters Using CHO Cell Lines Expressing the Green Fluorescent Protein
38. Na+ Is Responsible for Intra-Run Variations of Specific Productivity of CHO Cell Lines Producing Human Anti Rh D IgG in Bioreactors
39. 100 Liter Transient Transfection
40. Development of Pilot-Scale Orbital Shake Bioreactors: Ideal for Cost-Effective and Efficient Transient Gene Expression
41. Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells
42. kLa as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors
43. Manufacture of Recombinant Therapeutic Proteins Using Chinese Hamster Ovary Cells in Large-Scale Bioreactors
44. Principles of a Scaleable Transient Transfection and Expression Technology for Mammalian Cells
45. Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
46. A simple high-yielding process for transient gene expression in CHO cells
47. Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors
48. A collagen-poly(lactic acid-co-ɛ-caprolactone) hybrid scaffold for bladder tissue regeneration
49. High Level Transient Expression in Mammalian Cells: Identification and Optimization of Physico-Chemical Parameters of the Calcium Phosphate Transfection Method
50. High-Titer Neutralizing Antibodies against the SARS-CoV-2 Delta Variant Induced by Alhydroxyquim-II-Adjuvanted Trimeric Spike Antigens
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