1. Design, synthesis, antimycobacterial activity and molecular docking studies of novel 3- (N-substituted glycinamido) benzoic acid analogues as anti tubercular agents.
- Author
-
Veeravarapu H, Tirumalasetty M, Kurati S, Wunnava U, and Krishna Kumar Muthyala M
- Subjects
- Antitubercular Agents chemical synthesis, Antitubercular Agents metabolism, Benzoates chemical synthesis, Benzoates metabolism, Catalytic Domain, Drug Design, Enzyme Inhibitors chemical synthesis, Enzyme Inhibitors metabolism, Glycine metabolism, Methyltransferases chemistry, Methyltransferases metabolism, Microbial Sensitivity Tests, Molecular Docking Simulation, Mycobacterium tuberculosis drug effects, Protein Binding, Antitubercular Agents pharmacology, Benzoates pharmacology, Enzyme Inhibitors pharmacology, Glycine analogs & derivatives, Glycine pharmacology
- Abstract
We have recently identified mycolic acid methyl transferase (MmaA1) enzyme inhibitors as potential antitubercular agents using in silico modelling techniques. In continuation of that study, we synthesised a series of novel 3-(N-substituted glycinamido) benzoic acid derivatives with an aim to optimise the lead molecule. The newly synthesised compounds were evaluated for their in vitro antimycobacterial activity against M. tuberculosis H
37 Rv. Among these, 5 compounds A3, A4, A5, A6 and A10 exhibited most potent activity with an MIC value of 1.6 μg/ml. Further molecular docking studies were carried out to investigate the binding mode of the ligands with MmaA1 protein. The docking studies revealed that the ligands made strong interactions with the catalytic site residues TRP30, TYR 32, GLY 71, TRP 74, GLY 76, ALA 77 and GLU 136 of MmaA1 protein. Druglikeness and leadlikeness properties of the compounds were also studied using computational tools. The results of in silico and in vitro studies indicate that these novel compounds are propitious leads for tuberculosis therapy., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF