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8 results on '"Wulansari, Endang Dwi"'

3. The protective activity against inflammation of Zingiber montanum rhizome isolate topically on BALB/c strain mice exposed to UV-B.

Author

Wulansari, Endang Dwi, Wahyuono, Subagus, Marchaban, and Widyarini, Sitarina

Subjects
*LIQUID chromatography, *ZINGIBER, *INFLAMMATION, *MICE, *METHOXY group
Abstract

One of the causes of topical inflammation is UV-B radiation, which causes erythema and edema of the skin. Zingiber montanum rhizome in Indonesia is known as a natural ingredient that has the potential to treat skin redness. This study was conducted to isolate and identify the active compound, as well as to determine the protective activity against inflammatory of isolate in BALB/c mice exposed to UV-B. The isolation stage begins with the extraction process by maceration, fractionation by liquid-liquid partition and chromatography. Identification by IR Spectrophotometry, C-NMR, and H-NMR. The protection test against inflammation used a mice skin model irradiated with UV-B, with 5 test groups, namely: control, UV-B, base, isolate, and positive control 2.5% hydrocortisone cream group. Statistical data analysis using a different test. The results showed that the isolates could be separated from fraction number 8. The partial structure of the isolates were aromatic compounds having hydroxyl and methoxy groups, which leads to the structure of ferulic acid compounds. The dorsal skinfold thickness of mice in the control, UV-B, base, isolate, and positive control groups of 2.5% hydrocortisone cream were 0.570 ± 0.056; 1.612 ± 0.250; 1.700 ± 0.556; 0.952 ± 0.127; 0.824 ± 0.144 cm, respectively. There were significant differences between all test groups. In this study, an active compound was isolated from Zingiber montanum rhizome which has the potential to provide protective activity against inflammation in mice skin models exposed to UV-B. [ABSTRACT FROM AUTHOR]

Published
2023
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8. UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN KANDUNGAN ANTOSIANIN TOTAL KULIT BUAH MANGGIS (Garcinia mangostana L)

Author

Supiyanti, Wiwin, Wulansari, Endang Dwi, and Kusmita, Lia

Subjects
antosianin, kulit buah manggis, Garcinia mangostana L, antioksidan, anthocyanin, rind of mangosteen, Garcinia mangostana L, antioxidants
Abstract

Mangosteen fruit with latin name Garcinia mangostana L. got the nickname Queen of tropical fruit (Tropical Fruits queen). Most of the mangosteen fruit is consumed in fresh condition. Mangosteen rind purple black. Mangosteen rind allegedly containing anthocyanin compounds. This study aimed to determine antioxidant activity of bark extracts of the mangosteen fruit and the total anthocyanin content in the rind of the mangosteen fruit. Object of research is that the mangosteen rind has been good to consume. Mangosteen rind mashed and then soaked with methanol containing 1% HCl. Antioxidant activity test mangosteen rind extract includes a qualitative test of antioxidant activity of extracts of TLC plates sprayed mangosteen rind 0.1 mM DPPH reagent produces patches of pale yellow with a purple background. Quantitative test of antioxidant activity based on the parameters EC50 using DPPH method with the comparison of vitamin C. Obtained EC50 value of 8.5539 tg/mL and EC50 values of vitamin C is 3.3676 tg/mL. Average total anthocyanin content in the skin of the mangosteen fruit is 59.3 mg in 100 grams of the mangosteen rind., Buah manggis dengan nama latin Garcinia mangostana L. mendapat julukan Queen of tropical fruit (Ratunya Buah-buahan Tropik). Kulit buah manggis berwarna ungu kehitaman. Diduga kulit buah manggis mengandung senyawa antosianin. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak kulit buah manggis dan kandungan antosianin total dalam kulit buah manggis. Obyek penelitian adalah kulit buah manggis yang sudah layak untuk dikonsumsi. Kulit buah manggis dihaluskan kemudian direndam dengan pelarut metanol yang mengandung HCl 1%. Dilakukan uji aktivitas antioksidan menggunakan metode DPPH dan penentuan kandungan antosianin total dalam kulit buah manggis menggunakan metode perbedaan pH. Uji aktivitas antioksidan ekstrak kulit buah manggis meliputi uji aktivitas antioksidan secara kualitatif berupa lempeng KLT ekstrak kulit buah manggis disemprotkan penampak bercak DPPH 0,1 mM menghasilkan bercak kuning pucat dengan latar belakang ungu. Uji aktivitas antioksidan secara kuantitatif berdasarkan parameter EC50 menggunakan metode DPPH dengan pembanding vitamin C. Diperoleh nilai EC50 sebesar 8,5539 μg/mL dan nilai EC50 vitamin C adalah 3,3676 μg/mL. Rata-rata kandungan antosianin total dalam kulit buah manggis adalah 59,3 mg dalam 100 gram kulit buah manggis.

Published
2015

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