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Abstract Walking pace is a simple and functional form of exercise and a strong predictor of health, but little is known about its causal association with rheumatoid arthritis. This study aimed to investigate the causal effect of WP on the developing RA using Mendelian randomization analysis. The genetic variation associated with WP was selected as an instrumental variable from the latest genome-wide association studies. Summary-level data for the outcomes were obtained from the corresponding GWAS. The inverse-variance weighted method was used as the primary MR analysis. The results were further tested using a multivariable MR approach based on Bayesian model averaging. Confounders (BMI, SMK, HBP, TD) with close associations with RA were included in the analysis. An observational study with individual data from UK Biobank was performed to reinforce our findings. The MR results indicated the significant inverse associations of WP with the risk of RA (odds ratio (OR), 0.31; 95% confidence interval (CI), 0.15, 0.62; p = 1.05 × 10 −3). After adjusting for the risk factors, the associations for WP and RA did not change substantially. Observational study results demonstrated the same effect of WP on reducing the risk of RA. The Mendelian randomization analysis and observational study provide evidence suggesting that walking pace is a protective factor for rheumatoid arthritis. Given its simple measurement, walking pace may be a pragmatic target for interventions.

Abstract To study a new method for establishing animal models of prenatal bronchopulmonary dysplasia (BPD), we used lung ultrasound score (LUS) to semi-quantitatively assess the severity of lung lesions in model rats. Lipopolysaccharide (LPS) was injected into the right lung of the fetus of the rat under ultrasound-guided, and the right lung of the neonates were scanning for LUS. Specimens were collected for pathological scoring and detection of pulmonary surfactant-associated glycoprotein (SP)-C and vascular endothelial growth factor (VEGF) expression quantity. The correlation between LUS and pathological scores was analyzed. (1) The animal models were consistent with the pathological manifestations of BPD. (2) It showed a strong positive correlation between LUS and pathological scores in animal models (r = 0.84, P

The high energy density advantage of lithium (Li) metal batteries (LMBs) makes them increasingly desirable; however, problems such as strong reactivity and dendrite growth of Li metal anode limit their practical uses. In this work, a novel Li‐containing glycerol (LiGL) or lithicone protection layer on a 50 μm thick Li metal anode is employed for improving the performance of LMBs. This LiGL layer was accurately deposited via a molecular layer deposition (MLD) process at 150 °C, using lithium tert‐butoxide and glycerol as precursors. The as‐formed LiGL coating layer is highly tunable in its thickness by simply adjusting MLD cycles and shows a good stability and outstanding ionic transport properties. The LiGL layer is found to effectively mitigate side reactions and enhance cycling stability in both symmetric cells and full cells. Specifically, the LMBs with LiGL@Li anode of 400 MLD cycles and LiNi0.6Mn0.2Co0.2O2 cathode enable a capacity retention of ≈87%, much higher than ≈35% of the cells with bare Li after 200 cycles at a charge/discharge current density of 2.1 mA cm−2. This work paves a feasible way for practical LMBs with improved capacity and stability through applying an innovative protection layer on Li metal anodes.

Abstract Objectives While lipid metabolism disorder is widely acknowledged as a contributing factor to inflammation, the association between remnant cholesterol (RC), which indicates lipid metabolism, and rheumatoid arthritis (RA) has not been investigated. Accordingly, this study evaluated whether RC is associated with RA disease events. Methods Data were collected and specifically extracted from the National Health and Nutrition Examination Survey (NHANES) 1999–2008 database. The RC value was derived by subtracting the combined amount of low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) from the total cholesterol (TC). The association between RC and RA was evaluated using multivariate regression analysis and subgroup analysis. Results The study analyzed 7777 patients, of which 581 patients (7.47%) were diagnosed with RA. After accounting for different covariates, the multivariate logistic regression analysis revealed a notable correlation between increased RC levels and an increased likelihood of RA (odds ratio OR = 1.54; 95% confidence interval CI: 1.11–2.13; P = 0.0092). The interaction test did not yield statistically significant effects on this association. The linear correlation between RC and RA was observed within restricted cubic spline regression model limitations. Conclusion The results suggest that higher RC levels are associated with increased odds of RA, indicating that RC can serve as a novel and convenient index for forecasting the likelihood of RA in the United States. Additionally, these findings offer insights into early intervention strategies for susceptible populations at risk of developing RA.

Background: Incidental gall bladder cancer (IGBC) is often discovered unexpectedly in patients after cholecystectomy. Currently, magnetic resonance imaging (MRI) has been widely applied in the pre-operative diagnosis of gall bladder diseases as laparoscopic cholecystectomy developed into the preferred method. Aims and Objectives: This study aimed to evaluate the pre-operative MRI application and laparoscopic management in the IGBCs. Materials and Methods: Between January 2011 and January 2020, a total of 7917 patients with gall bladder diseases treated by laparoscopy were enrolled in this study. Results: Amongst 49 patients diagnosed with IGBCs, the incidence of IGBCs in polypoid lesions, biliary pancreatitis, cholecystitis, cholecystocholedocholithiasis and gall bladder stones was 0.42%, 1.19%, 0.62%, 1.20% and 0.49%, respectively. MRI evaluation showed more remarkable pre-operative imaging as compared to ultrasonographic evaluation (40.8 vs. 26.5, P < 0.05). Furthermore, 14 patients were diagnosed with gall bladder cancer through intraoperative histological examination and 11 received laparoscopic extensive resection after cholecystectomy. MRI findings with diffuse thickening of the gall bladder detected IGBCs with 6.1% sensitivity, 96.02 specificity, 0.95% positive predictive values and 99.4% negative predictive values; diffuse thickening of the gall bladder with suspicion of malignancy detected IGBCs with 12.2% sensitivity, 99.1% specificity, 7.6% positive predictive values and 99.5% negative predictive values; focal thickening of the gall bladder detected IGBCs with 16% sensitivity, 99.8% specificity, 32% positive predictive values and 99.5% negative predictive values; moreover, suspicious lesion detected IGBCs with 6.1% sensitivity, 99.6% specificity, 8.8% positive predictive values and 99.4% negative predictive values. Conclusions: Patients with biliary pancreatitis and cholecystocholedocholithiasis have a higher incidence of IGBC. MRI evaluation could provide more accurate information for the IGBCs, which should be recommended for patients accepting cholecystectomy. MRI findings exhibited an unsatisfactory sensitivity when detecting IGBCs, but they represented high specificity. Pre-operative MRI evaluation and intraoperative histological examination may help some IGBCs to achieve one-stage laparoscopic extensive resection.

Abstract Background Gastric cancer (GC) is considered the sixth highly prevailing malignant neoplasm and is ranked third in terms of cancer mortality rates. To enable an early and efficient diagnosis of GC, it is important to detect the fundamental processes involved in the oncogenesis and progression of gastric malignancy. The understanding of molecular signaling pathways can facilitate the development of more effective therapeutic strategies for GC patients. Methods The screening of genes that exhibited differential expression in early and advanced GC was performed utilizing the Gene Expression Omnibus databases (GSE3438). Based on this, the protein and protein interaction network was constructed to screen for hub genes. The resulting list of hub genes was evaluated with bioinformatic analysis and selected genes were validated the protein expression by immunohistochemistry (IHC). Finally, a competing endogenous RNA network of GC was constructed. Results The three genes (ITGB1, LUM, and COL5A2) overexpressed in both early and advanced GC were identified for the first time. Their upregulation has been linked with worse overall survival (OS) time in patients with GC. Only LUM was identified as an independent risk factor for OS among GC patients by means of additional analysis. IHC results demonstrated that the expression of LUM protein was increased in GC tissue, and was positively associated with the pathological T stage. LUM expression can effectively differentiate tumorous tissue from normal tissue (area under the curve = 0.743). The area under 1-, 3-, and 5-year survival relative operating characteristics were greater than 0.6. Biological function enrichment analyses suggested that the genes related to LUM expression were involved in extracellular matrix development-related pathways and enriched in several cancer-related pathways. LUM affects the infiltration degree of cells linked to the immune system in the tumor microenvironment. In GC progression, the AC117386.2/hsa-miR-378c/LUM regulatory axis was also identified. Conclusion Collectively, a thorough bioinformatics analysis was carried out and an AC117386.2/hsa-miR-378c/LUM regulatory axis in the stomach adenocarcinoma dataset was detected. These findings should serve as a guide for future experimental investigations and warrant confirmation from larger studies.

Krüppel-like zinc-finger transcription factor 5 (KLF5) is a vital regulator of breast cancer (BC) onset and progression. The mechanism by which KLF5 regulates BC is still not clearly known. In this study, bioinformatics analysis shows that BC-affected individuals with elevated KLF5 expression levels have poor clinical outcomes. We further verify that miR-145-5p regulated KLF5 expression to promote cell apoptosis and inhibit cell proliferation in BC via dual-luciferase reporter assay, western blot analysis, qRT-PCR, CCK-8 assay and cell apoptosis assay. In addition, based on bioinformatics analysis, the binding of ENST00000422059 with miR-145-5p is confirmed by dual-luciferase reporter assay. Subsequently, FISH, western blot analysis, qRT-PCR, CCK-8 and cell apoptosis assays verified that ENST00000422059 increases KLF5 protein expression by sponging miRNA to promote cell proliferation and inhibit cell apoptosis. Finally, ENST00000422059 is found to accelerate tumor progression by regulating the miR-145-5p/KLF5 axis in vivo. In conclusion, this study suggests that ENST00000422059 upregulates KLF5 by sponging miR-145-5p to promote BC progression.

Precise wind power forecasting is essential for the successful integration of wind power into the power grid and for mitigating the potential effects of wind power on the power system. To enhance the precision of predictions, a hybrid VMD-BiTCN-Psformer model was devised. Firstly, VMD divided the original sequence into several data components with varying time scales. Furthermore, the BiTCN network was utilized to extract the sequence features. These features, along with the climate features, were then input into the positional encoding and ProbSparse self-attention improved Transformer model. The outputs of these models were combined to obtain the ultimate wind power prediction results. For the prediction of the wind power in Fujian Province on April 26, four additional models were developed for comparison with the VMD-BiTCN-Psformer model. The VMD-BiTCN-Psformer model demonstrated the greatest level of forecast accuracy among all the models. The R2 increased by 22.27%, 12.38%, 8.93%, and 2.59%, respectively.

The output power of PV systems is influenced by various factors, resulting in strong volatility and randomness, which makes it difficult to forecast. Therefore, this paper proposes an Informer prediction model based on optimised VMD for predicting short-term PV power. Firstly, the temporal coding of the Informer model is improved and, secondly, the original sequence is decomposed into multiple modal components using VMD, and then optimisation of the results of VMD in conjunction with the optimisation strategy of SSA improves the characteristics of the time series data. Finally, the refined data are fed into the Informer framework for modelling and prediction, utilising the self-attention mechanism and multiscale feature fusion of Informer to precisely forecast PV power. The power of PV prediction data from the SSA-VMD-Informer model and four other commonly used models is compared. Experimental results indicate that the SSA-VMD-Informer model performs exceptionally well in short-term PV power prediction, achieving higher accuracy than traditional methods. As an example, the results of predicting the PV power on 24 April in a region of Xinjiang are 1.3882 for RMSE, 0.8310 for MSE, 1.14 for SDE, and 0.9944 for R2.

Diesel engine combustion is dependent mainly on the fuel injection characteristics, particularly the injection pressure and rate, which directly affect the engine efficiency and emissions. Herein, an electrically controlled supercharger is added to a traditional high-pressure common rail system to form an ultrahigh-pressure common rail system. Then, the variations in the spray, combustion, and emission characteristics of a diesel engine with a variable fuel injection rate are analyzed. Moreover, a simulation model for a diesel engine combustion chamber is built and verified by experimental results for numerical analysis. The results reveal that the injection rate can be flexibly adjusted via regulation when the solenoid valves are opened on the electrically controlled supercharger. Specifically, (1) the boot-shaped injection rate has greater potential than the traditional rectangular injection rate in terms of combustion and emission; (2) the main injection advance angle at the boot-shaped injection rate can be properly increased to improve combustion; and (3) the pilot injection quantity and advance angle are strongly coupled with the boot-shaped injection rate, potentially enhancing the mixing efficiency of fuel and air in the cylinder to achieve favorable emission results. This paper provides good guidance for the reliable design and optimization of noble-metal-based diesel engines.

Abstract Objectives Insulin resistance is a well-established contributor to inflammation; however, the specific association between the triglyceride glucose (TyG) index, a biomarker reflecting insulin resistance, and arthritis remains unexplored. As a result, the main aim of this study was to examine the correlation between the TyG index and arthritis. Methods This observational study used data from the National Health and Nutrition Examination Survey (NHANES), which was conducted between 2007 and 2018. To investigate the relationship between the TyG index and arthritis, various statistical analyses were employed, including weighted multivariable logistic regression analysis, subgroup analysis, curve fit analysis, and threshold effect analysis. Results In total, 14,817 patients were enrolled in the trial, with 4,191 individuals (28.29%) diagnosed with arthritis. An increased risk of arthritis was found to be significantly correlated with higher TyG index values (odds ratio OR = 1.15, 95% confidence interval CI: 1.07–1.23), according to the results of multivariable logistic regression analysis after full adjustment. Subgroup analysis and interaction tests further indicated that the TyG index exhibited an additive effect when combined with other established risk factors, including age (OR = 1.29; 95% CI: 1.17–1.41), body mass index (BMI) (OR = 1.43; 95% CI: 1.24–1.69), and diabetes (OR = 1.20; 95% CI: 1.11–1.31). Additionally, curve fit analysis and threshold effect analysis demonstrated a nonlinear relationship with a breakpoint identified at 8.08 µmol/L. Conclusion The TyG index was positively correlated with arthritis in adults under 60 years of age in the United States who had normal weight and no diabetes. Further large-scale prospective studies are warranted for a comprehensive analysis of the role of the TyG index in arthritis.

Accurate wind power forecasting is essential for both optimal grid scheduling and the massive absorption of wind power into the grid. However, the continuous changes in the contribution of various meteorological features to the forecasting of wind power output under different time or weather conditions, and the overlapping of wind power sequence cycles, make forecasting challenging. To address these problems, a short-term wind power forecasting model is established that integrates a gated recurrent unit (GRU) network with a dual attention mechanism (DAM). To compute the contributions of different features in real time, historical wind power data and meteorological information are first extracted using a feature attention mechanism (FAM). The feature sequences collected by the FAM are then used by the GRU network for preliminary forecasting. Subsequently, one-dimensional convolution employing several distinct convolution kernels is used to filter the GRU outputs. In addition, a multi-head time attention mechanism (MHTAM) is proposed and a Gaussian bias is introduced to assign different weights to different time steps of each modality. The final forecast results are produced by combining the outputs of the MHTAM. The results of the simulation experiment show that for 5-h, 10-h, and 20-h short-term wind power forecasting, the established DAM-GRU model performs better than comparative models on the basis of Root Mean Square Error (RMSE), Mean Absolute Error (MAE), R-squared (R2), Square sum error (SSE), Mean absolute percentile error (MAPE), and Relative root mean square error (RRMSE) index.

This study establishes an analytical method for simultaneous determination of 19 Succinate dehydrogenase inhibitor fungicides pesticide residues in Green coffee by QuEChERS-Liquid chromatography tandem mass spectrometry. The Green coffee powder samples were extracted with acetonitrile by Homogenizer, the supernatant were purified by Magnesium sulfate anhydrous、PSA and C18, liquid chromatograhy - tandem mass spectrometry were used for analyzing 19 Succinate dehydrogenase inhibitor fungicides pesticide residues at positive ion mode. The results showed that the calibration curves of 19 targets showed good linearity with correlation coefficients of above 0.995 0 in the range of 1~100 ng/mL, the recoveries of 19 pesticides were 79.6%~109.4% and the relative standard deviations(RSD, n=6) were in the range of 1.4%~9.7% at spiked levels of 0.01、0.05 and 0.1 mg/kg. Limit of quantitation (LOQ) of all the tested pesticides were 0.51~4.60 μg/kg. This method has good linear relations, excellent stable and sensitive, accuracy and precision meet the requirement of quantitative analysis, it is suitable for quantitative analysis of 19 Succinate dehydrogenase inhibitor fungicides pesticide residues in Green coffee.

Abstract Background Orthostatic state is maintained by harmonizing the spine, pelvis and lower extremities. In the past few decades, several studies have demonstrated the associations between spinal imbalance and generalized osteoarthritis. The compensatory mechanisms of pelvis translation and knee flexion, however, have not been fully assessed. Methods A total of 213 volunteers, over 40 years of age, were recruited. Radiological measurements were performed by EOS imaging system. Pelvic tilt (PT), pelvic incidence (PI), lumbar lordosis (LL), sagittal vertical axis (SVA), global tilt (GT), hip-knee-angle (HKA), knee flexion angle (KFA), lateral distal femoral angle (LDFA), and medial proximal tibial angle (MPTA) were measured. On the basis of SRS-Schwab, the subjects were classified into decompensated group (PI-LL > 20°), compensated group(10° ≤ PI-LL ≤ 20°), and normal group (PI-LL

Photosystem I (PS I) is a photosynthetic pigment–protein complex that absorbs light and uses the absorbed energy to initiate electron transfer. Electron transfer has been shown to occur concurrently along two (A- and B-) branches of reaction center (RC) cofactors. The electron transfer chain originates from a special pair of chlorophyll a molecules (P700), followed by two chlorophylls and one phylloquinone in each branch (denoted as A−1, A0, A1, respectively), converging in a single iron–sulfur complex Fx. While there is a consensus that the ultimate electron donor–acceptor pair is P700+A0−, the involvement of A−1 in electron transfer, as well as the mechanism of the very first step in the charge separation sequence, has been under debate. To resolve this question, multiple groups have targeted electron transfer cofactors by site-directed mutations. In this work, the peripheral hydrogen bonds to keto groups of A0 chlorophylls have been disrupted by mutagenesis. Four mutants were generated: PsaA-Y692F; PsaB-Y667F; PsaB-Y667A; and a double mutant PsaA-Y692F/PsaB-Y667F. Contrary to expectations, but in agreement with density functional theory modeling, the removal of the hydrogen bond by Tyr → Phe substitution was found to have a negligible effect on redox potentials and optical absorption spectra of respective chlorophylls. In contrast, Tyr → Ala substitution was shown to have a fatal effect on the PS I function. It is thus inferred that PsaA-Y692 and PsaB-Y667 residues have primarily structural significance, and their ability to coordinate respective chlorophylls in electron transfer via hydrogen bond plays a minor role.

The involvement of the second pair of chlorophylls, termed A-1A and A-1B, in light-induced electron transfer in photosystem I (PSI) is currently debated. Asparagines at PsaA600 and PsaB582 are involved in coordinating the A-1B and A-1A pigments, respectively. Here we have mutated these asparagine residues to methionine in two single mutants and a double mutant in PSI from Synechocystis sp. PCC 6803, which we term NA600M, NB582M, and NA600M/NB582M mutants. (P700+–P700) FTIR difference spectra (DS) at 293 K were obtained for the wild-type and the three mutant PSI samples. The wild-type and mutant FTIR DS differ considerably. This difference indicates that the observed changes in the (P700+–P700) FTIR DS cannot be due to only the PA and PB pigments of P700. Comparison of the wild-type and mutant FTIR DS allows the assignment of different features to both A-1 pigments in the FTIR DS for wild-type PSI and assesses how these features shift upon cation formation and upon mutation. While the exact role the A-1 pigments play in the species we call P700 is unclear, we demonstrate that the vibrational modes of the A-1A and A-1B pigments are modified upon P700+ formation. Previously, we showed that the A-1 pigments contribute to P700 in green algae. In this manuscript, we demonstrate that this is also the case in cyanobacterial PSI. The nature of the mutation-induced changes in algal and cyanobacterial PSI is similar and can be considered within the same framework, suggesting a universality in the nature of P700 in different photosynthetic organisms.

Ultra-flat carrying robots (UCR) are used to carry soft targets for functional safety road tests of intelligent driving vehicles and should have superior control performance. For the sake of analyzing and upgrading the motion control performance of the ultra-flat carrying robot, this paper develops the mathematical model of its motion control system on the basis of the test data and the system identification method. Aiming at ameliorating the defects of the standard particle swarm optimization (PSO) algorithm, namely, low accuracy, being susceptible to being caught in a local optimum, and slow convergence when dealing with the parameter identification problems of complex systems, this paper proposes a refined PSO algorithm with inertia weight cosine adjustment and introduction of natural selection principle (IWCNS-PSO), and verifies the superiority of the algorithm by test functions. Based on the IWCNS-PSO algorithm, the identification of transfer functions in the motion control system of the ultra-flat carrying robot was completed. In comparison with the identification results of the standard PSO and linear decreasing inertia weight (LDIW)-PSO algorithms, it indicated that the IWCNS-PSO has the optimal performance, with the number of iterations it takes to reach convergence being only 95 and the fitness value being only 0.117. The interactive simulation model was constructed in MATLAB/Simulink, and the critical proportioning method and the IWCNS-PSO algorithm were employed respectively to complete the tuning and optimization of the Proportional-Integral (PI) controller parameters. The results of simulation indicated that the PI parameters optimized by the IWCNS-PSO algorithm reduce the adjustment time to 7.99 s and the overshoot to 13.41% of the system, and the system is significantly improved with regard to the control performance, which basically meets the performance requirements of speed, stability, and accuracy for the control system. In conclusion, the IWCNS-PSO algorithm presented in this paper represents an efficient system identification method, as well as a system optimization method.

Wind power prediction values are often unstable. The purpose of this study is to provide theoretical support for large-scale grid integration of power systems by analyzing units from three different regions in China and using neural networks to improve power prediction accuracy. The variables that have the greatest impact on power are screened out using the Pearson correlation coefficient. Optimize LSTM with Lion Swarm Algorithm (LSO) and add GCT attention module for optimization. Short-term predictions of actual power are made for Gansu (Northwest China), Hebei (Central Plains), and Zhejiang (Coastal China). The results show that the mean absolute percentage error (MAPE) of the nine units ranges from 9.156% to 16.38% and the root mean square error (RMSE) ranges from 1.028 to 1.546 MW for power prediction for the next 12 h. The MAPE of the units ranges from 11.36% to 18.58% and the RMSE ranges from 2.065 to 2.538 MW for the next 24 h. Furthermore, the LSTM is optimized by adding the GCT attention module to optimize the LSTM. 2.538 MW. In addition, compared with the model before data cleaning, the 12 h prediction error MAPE and RMSE are improved by an average of 34.82% and 38.10%, respectively; and the 24 h prediction error values are improved by an average of 26.32% and 20.69%, which proves the necessity of data cleaning and the generalizability of the model. The subsequent research content was also identified.

Objective: Aims at the brittleness regulation of fruit and vegetable slices during hot air drying process. Methods: This article takes fresh potatoes and bananas as the research materials, adopts mechanical and acoustic measuring devices to collect mechanical and acoustic signals generated by the fruit and vegetable slice samples at the mechanical compression mode, which were collected at different drying times during the hot air drying process of 80 ℃, to comprehensively evaluate the fragility of the fruit and vegetable slices. Results: The acoustic emission （AE） signal of the potato slice appeared the drying time of 45 min, and its AE energy increased gradually with the increase of drying time. The stress increased rapidly and then remained stable; The best drying end time of potato was 180 min. At this time, the AE energy and force were 255.38 mV·ms and 20.25 N respectively. The AE signal of the banana slices appeared from the early stage of drying, and its energy increased rapidly with the progress of drying, decreased after 150 min of drying, and remained stable after 200 min. The stress appeared after drying for 60 min, increased rapidly until the peak at 240 min, and remained stable. The optimum drying end time of banana slices was 240 min, and the energy and force were 122.58 mV·ms and 18.01 N, respectively. Conclusion: The mechanical-acoustic detecting method can be used to monitor the crispness variation of the fruit and vegetable slices being dried and to optimize the hot air drying process.

Shanghai Synchrotron Radiation Facility (SSRF) is a third-generation light source that has been operating steadily and consistently providing high brightness X-rays to multidisciplinary fields for over a decade. This achievement is mainly attributed to the long-term stability of electron beam parameters, among them, the linear optical model is the most important to guarantee the stability of light source properties and the stable operation. The brightness of synchrotron radiation, beam emittance, beam lifetime and other important parameters are directly affected by the linear optical functions. In SSRF, the tune feedback system based on the response matrix method was tested in the first half of 2019 and then put into operation during normal user time. This feedback system can not only correct the betatron tunes online, but also realize the dimension reduction correction of linear optical functions. The stable operation of the tune feedback system in SSRF has proved the feasibility of this method. The results of operation in several weeks show that the feedback system not only significantly improves the stability of the tunes, but also improves the stability of important beam parameters such as beam emittance, injection efficiency and beam lifetime. In addition, diffraction-limited storage ring (DLSR) light sources with higher brightness and transverse coherence are being widely studied and constructed worldwide. The round beam mode based on linear difference coupling resonance will be used by some DLSR facilities to optimize the matching of photon and electron beams in phase space and to improve the beam lifetime. The stable operation of this kind of round beam mode highly requires the stability of betatron tunes. Therefore, it is very important to improve the stability of betatron tunes for the stable operation of the third-generation synchrotron radiation light sources and more important for DLSRs. The feasibility of the tune feedback system on the new generation of light sources needs to be verified. For this purpose, the tune feedback system on the SSRF-U storage ring lattice was simulated, where SSRF-U is an important alternative for upgrading SSRF to diffraction-limited storage ring with an extremely low natural emittance of 53.2 pm·rad. The simulation results show that the tune stability of SSRF-U is better than ±0.001 under the action of the tune feedback. Such a high tune stability is enough to meet the needs of the linear difference coupling resonance round beam mode in DLSRs for the stability operation. In summary, this paper analyses the principle of tune feedback, reviews the operation of SSRF tune feedback system, and introduces the simulation of tune feedback on SSRF-U.

in order to better promote the transportation industry to reduce carbon emissions and improve the competitiveness of railway freight in the whole freight market, to achieve the “double carbon” strategy and promote the “transit rail”. In this paper, considering the dynamic pricing of freight volume and freight price, a two-layer programming model is established under the minimum social total cost of railway and road. The upper level considers the income of railway freight, and the lower level considers the total social cost of freight transportation, so as to realize the increase of railway freight revenue and the decrease of the general total expenditure paid by freight owners. Considering the transportation of bulk commodity freight from shandong to sichuan, it is concluded that the railway freight market has a good development prospect under the consideration of the total social expenditure such as carbon emission. Under the carbon emission charge, the appropriate increase of railway freight rate will not lead to the reduction of railway freight volume. As rail freight becomes more competitive under increasingly stringent carbon emissions penalties, the volume of rail freight will continue to rise even after appropriate increases in rail freight fees. At this point, rail freight companies can transport more goods and gain higher returns, slightly increasing the overall cost borne by shippers in a broad sense, and helping to reduce carbon emissions in the process of freight transport.

RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation. In this study, we identified TaGW2L, encoding a Grain width and weight2 (GW2)-like RING finger E3 ligase, as a novel positive regulator of heading date in wheat (Triticum aestivum L.). TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs, particularly in the conserved RING finger domain. Expression analysis indicated that TaGW2L was constitutively expressed in various wheat tissues. TaGW2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2s. An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to TaGW2. Overexpression of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day conditions. Transcriptome analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type control. Together, our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.

Microvascular decompression (MVD) is a widely adopted neurosurgery in treating cranial nerve diseases providing long-term pain relief. Improving surgical techniques has been a focus of recent studies. Venous structures such as the sigmoid sinus are essential to protect, and whose risk of destruction during surgery increases with size. The medical records of patients who went through MRI ahead of MVD surgery between Dec 2020 and Dec 2021 were reviewed. Section area of sigmoid sinus calculated from the MRI plane of auditory nerve showed a right dominance of the sinus. The improved method concerning the relationship between affected side and the dominant sigmoid sinus offered a better bone window and surgical field by planning the operation incision in advance. Intraoperative adjustment of the bone flap was avoided, and the risk of destructing the sigmoid sinus was reduced.

This study presents a novel shock absorber with an inward-inverting composite foam-filled tube. Under the compression of a pressing cap and the action of an internal inversion cap, the composite tube inverted inward. During the crushing, the fronds of the composite tube compacted the foam, thereby enhancing the energy absorption. Three types of foams were applied to the absorber, and a drop-weight impact test was performed to obtain the assessment parameters. The foam increased the specific energy absorption (SEA) of the composite tube to 103 kJ/kg. Finite-element simulation based on the user-defined material subroutine was performed for the initial failure and stable stages of the crushing, and a foam model was identified through the experimental data. The mean crush force from the simulation agreed with the experimental data, and the SEA maximum error was

AIM: To demonstrate an improved surgical technique of whole piece consecutive internal limiting membrane (ILM) peeling without preservation of the epi-fovea to treat high myopic foveoschisis (MF). METHODS: A 23-gauge 3-port pars plana vitrectomy was performed on 16 patients with high MF. A parallel arc line along the vascular arcades was scraped out with a curved membrane scraper DSP. Next, an ILM forceps was used to catch hold of the incisal edge of the ILM flap, and the action of releasing and separating was subsequently taken toward the direction of the macular fovea. Next, the ILM forceps was used to grasp the released area, and the whole area coherent ILM peeling covering the macular fovea was implemented thereafter. Finally, the ILM was folded backwards and peeled off in the arc direction. RESULTS: At the final visit, the average central macular thickness decreased remarkably from 423.76±177.67 to 178.24±66.21 μm. The mean logarithm of the minimum angle of resolution best-corrected visual acuity of 1.37±0.59 was significantly alleviated to 0.74±0.59. CONCLUSION: The wide range of whole piece consecutive ILM peeling without preservation of the epi-fovea is proven to be effective and significantly reduced the occurrence of retinal tear and macular hole.

This review summarized the research status of NADES on the extraction and separation of phenols, discussed the main factors affecting the extraction efficiency, and prospected the future development direction.

We have developed an alignment-free TSR (Triangular Spatial Relationship)-based computational method for protein structural comparison and motif identification and discovery. To demonstrate the potential applications of the method, we have generated two datasets. One dataset contains five classes: Actin/Hsp70, serine protease (chymotrypsin/trypsin/elastase), ArsC/Prdx2, PKA/PKB/PKC, and AChE/BChE at the hierarchical level 1 and twelve groups at the level 2. The other dataset includes representative proteases and ACE/ACE2. The x,y, z coordinates of the structures were obtained from PDB. We calculated the keys (or features) that represent each structure using the TSR-based method. The dataset and data presented here include additional information that help the readers become aware of specific applications of the TSR-based method in protein clustering, identification and discovery of metal ion binding sites as well as to understand the effect of amino acid grouping on protein 3D structural relationships at both global and local levels.

Determining crustal stress is crucial in the design and construction of underground engineering projects, particularly in fault fracture zones. This paper aims to identify the characteristic factors that influence the crustal stress of faults, including the difference in deformation characteristics between faults and ordinary formations (expressed by the elastic modulus ratio Er/Ef), the degree of fault fragmentation P, and the angle α between fault tendency and the principal stress direction. Numerical simulations are conducted to investigate the impact of these three factors on the magnitude and direction of the principal stress. Additionally, measured stress values from a specific tunnel are analyzed to validate the findings. The results demonstrate the crucial role of α in determining the increase or decrease of principal stress and the direction of the offset. When α is less than 45°, σ1 and σ3 within the fault experience an increase, while σ1 and σ3 in the ordinary surrounding rock area near the fault exhibit a slight decrease. Moreover, the σ1 direction inside the fault deviates towards the parallel fault, whereas σ1 near the outside slightly deviates towards the vertical fault. Conversely, when α is greater than 45°, the trend of principal stress is reversed. Er/Ef affects the magnitude of the principal stress change, with larger values resulting in more pronounced changes. P only affects the stress distribution inside the fault.

The closed section of the inverted arch, formed by the surrounding rock, acts as a bearing ring. Combined with the upper initial support, it ensures stable initial support. However, excavating the inverted arch can disturb the original balance, significantly affecting the tunnel’s stability. To determine the optimal exposure length and excavation length of the elevation arches at different rock levels, numerical analyses were conducted. These analyses used the classical Burgers creep intrinsic structure model for the three-step excavation mode. Various closure distances and exposure distances of the elevation arch were considered. The study aimed to investigate the influence of these factors on the stability of the primary lining, comparing it with the maximum displacement of the vault. The results indicate that the strength of the surrounding rock primarily affects the displacement of the arch crown. Lower rock strength corresponds to greater arch crown displacement. Additionally, increasing the closure distance of the inverted arch leads to increased arch displacement. On the other hand, the exposure distance of the inverted arch has minimal impact on arch displacement. Longer exposure distances result in greater arch displacement. These findings can serve as a basis for improving current standards and adapting them to meet the spatial requirements of large-scale mechanized operations.

In Austen Angell's long and fruitful scientific career, electrolyte research represented a brief and temporary “tangent” that deviated from his normal trajectory of interest. However, with this tangential touch, he left profound and rich legacies in the understanding of fundamental aspects of electrolyte science, the ripple effect of which can still be felt today.

This study aimed to isolate and characterize the structures of glycoproteins from peas and determine their hypoglycemic activity. The crude pea glycoproteins (PGP) were extracted by hot water and purified by diethylaminoethyl (DEAE)-Sepharose chromatography and Sephadex G-100 size-exclusion chromatography in sequence. Then three main fractions were obtained, namely PGP1, PGP2 and PGP3, with molecular weights of 897 615, 846 740 and 1 194 692 Da, respectively. The physical and chemical properties of the three fractions were evaluated and compared by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), scanning electron microscope (SEM), high performance liquid chromatography (HPLC) and other analytical techniques. The fraction PGP2 with the highest hypoglycemic activity, was screened using the Caco-2 monolayer cell model. It can inhibit the uptake of glucose in the small intestine, as well as the activities of maltase and sucrase. After simulated gastrointestinal digestion, PGP2 significantly enhanced the inhibitory effect of α-glucosidase, and slightly reduced the inhibitory ability of α-amylase. In summary, PGP2 possessed strong hypoglycemic activity after digestion. These results indicated that PGP2 has the potential to be developed into a functional food or natural medicine for the treatment of type 2 diabetes mellitus.

Lung adenocarcinoma (LUAD) is currently one of the most common malignant tumors worldwide. However, there is a lack of long noncoding RNA (lncRNA)-based effective markers for predicting the prognosis of LUAD patients. We identified four lncRNAs that can effectively predict the prognosis of LUAD patients.

The high-performance reduced graphene oxide/sulfur (rGO/S) composite cathode was obtained by integrating sulfur nanoparticles with graphene oxide (GO) in liquid phase reduction by self-assembly process. The morphology and microstructure of the materials were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM),X-ray diffractometer (XRD),Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). The results show that sulfur nanoparticles are uniformly distributed among the graphene sheets and effectively encapsulated by the graphene sheets. The mass fraction of sulfur in 35-rGO/S composite is as high as 83.6%. The initial discharge capacity of the 35-rGO/S composite cathode can reach 1197.3 mAh·g-1 at a current density of 0.2 C, and the capacity remains 730 mAh·g-1 after 200 cycles, manifesting outstanding cycling stability.

Background: We wondered whether the third-generation gamma nail-3 (GN-3) was better for junior surgeons to learn to treat geriatric intertrochanteric femur fractures than proximal femoral nail antirotation-II (PFNA-II). Methods: This is a prospective randomised study of 350 patients who underwent GN-3 fixation and PFNA-II carried out by junior orthopaedic trauma surgeons from January 2011 February 2017. We compared nail positioning, complication rates, operative and fluoroscopy time, blood transfused, time to mobilisation, hospital stay, fracture union, mismatch, mortality and postoperative outcomes. The minimum follow-up was 12 months (mean, 27.2 months; range, 12–42 months). Results: The recovery rate of the GN-3 group was higher than that of the PFNA-II group significantly. Compared with the PFNA-II group, the GN-3 group was superior in fracture gap, while operative time, fluoroscopy time, blood transfused, time to mobilisation, hospital stay, Harris Hip Score, reoperation, mortality and so on had no significant difference between two groups. There were five cases with cutout through the femoral neck in the GN-3 group, whereas in the PFNA-II group, we only had two cases with significant difference. The area of match in the GN-3 group conformed to that of the femur of Asian population better than that in the PFNA-II group. Conclusions: PFNA-II and GN-3 internal fixation are both effective methods for junior orthopaedic trauma surgeons to treat femoral intertrochanteric fracture. But our study reveals better results of the GN-3 group over the PFNA-II group on recovery rate. There is a high rate of cutout in patients treated with the GN-3, especially for those with bone defect or serious osteoporosis. The translational potential of this article: The results of this work have the potential to improve the cognition of geriatric intertrochanteric femur fractures for junior surgeons, supplying the theoretical basis for the selection and comparison of Intramedullary nail. Such a guidance will allow better healing, fewer complications, and ultimately improved outcomes. Keywords: Complications, Gamma nail-3, Geriatric intertrochanteric femur fractures, Junior surgeons, Proximal femoral nail antirotation-II, Recovery

Abstract Background Best surgical of recurrent anterior shoulder instability remained controversial. We knew little about the superiority and choice between traditional open and modern arthroscopic techniques. We hypothesized that outcomes of all patients will be similar regardless of surgical technique. Methods A retrospective case-cohort analysis of 168 patients who had recurrent anterior shoulder instability was conducted from September 2010 to December 2013. All cases (mean age 30.8 [range 18–50] years) were performed with arthroscopic Bankart repair (33 males/20 females), open Latarjet (34 males/18 females), and capsular shift (31 males/14 females). The average follow-up was 67.6 months (range 60–72). The shoulder instability index score (ISIS) was more than 3 with an average of 6.4. Results All treatments proved to be effective in improving shoulder functional status and reducing symptoms, while Latarjet had an advantage over subjective perception. The Rowe scores in arthroscopic Bankart, open Latarjet, and capsular shift group were 92.3 ± 1.5, 96.2 ± 2.1, and 93.2 ± 2.3, respectively, with significant difference. There was no significant difference in other functional outcomes. However, the Latarjet group in subjective results (subjective shoulder value (SSV) and subjective shoulder value for sport practice (SSV Sport)) was superior to the others (P

BACKGROUND As the world's largest producer of synthetic diamonds, China accounts for more than 90% of the world's total production, of which high pressure-high temperature (HPHT) synthetic diamonds are dominant. Previous scholars have conducted many studies on analytical methods and property analysis of HPHT synthetic diamonds. However, the research on the pickling process after the growth of HPHT synthetic diamonds and the internal and external micro-structures after pickling is still insufficient. OBJECTIVES To characterize 118 synthetic diamonds produced in China by high temperature and high pressure method. METHODS The HPHT synthetic diamonds were observed by laser scanning confocal microscopy (LSCM) and ultra-depth-of-field 3D microscopy, and the dark inclusions in these micro-structures were qualitatively analyzed by laser Raman spectroscopy. RESULTS Three structure types were found. Step-like growth structures with certain angles revealed the crystallographic characteristics of HPHT synthetic diamond poly-crystals. Near-parallel linear scratches reflected the contact relationship between diamond grains in the growth chamber. The 'rope-tied' structures reflected the growth rate of HPHT synthetic diamonds. CONCLUSIONS The micromorphology of these diamond surfaces reflects the growth conditions and environment of HPHT synthetic diamond gradient high temperature and continuous high pressure, which is of significance for the identification of HPHT synthetic diamonds.

We aimed to explore the role of capillary morphogenetic protein 2 (CMG2) in glioma cell invasion and the possible molecular mechanism. Glioma cells U87 and U251 stably overexpressing CMG2 were constructed by lentiviral transfection. The changes of cell invasion and migration were tested by Matrigel-transwell assay and scratch assay, respectively. A mouse model with orthotopically transplanted tumour was established to evaluate the effects of CMG2 overexpression on the in vivo invasion of glioma cells and survival time. The differences of filopodia and lamellar pseudopodia among glioma cells with different CMG2 expressions were observed by immunofluorescence assay. The expressions of YAP and p-YAP in glioma cells overexpressing CMG2 or not were compared by Western blot. Compared with the control group, overexpression of CMG2 enhanced the invasion and migration capacities of glioma cells (p < 0.05). The tumour tissues of mice transplanted with glioma cells overexpressing CMG2 were obviously invaded, and their survival time was significantly shortened (p < 0.05). Immunofluorescence staining showed that glioma cells overexpressing CMG2 formed more lamellipodia and filopodia than those of the control group. As glioma cells overexpressing CMG2 formed more pseudopodia, the expression of YAP, a key effector protein of the Hippo pathway, was up-regulated. CMG2 promoted the invasion of glioma cells, and may induce pseudopodium formation by up-regulating YAP expression.

NiO，Er2O3，Yb2O3，Y2O3 and ZrO2 powders were used as raw materials to prepare composite ceramic materials by the method of high temperature solid phase synthesis. The specific heat, thermal diffusion coefficient, thermal conductivity and other thermal physical properties of the composite ceramic materials were studied and compared with the traditional 8YSZ thermal barrier coating materials. The crystal structure and phase composition were tested by XRD. The results show that after NiO，Er2O3，Yb2O3，Y2O3 and ZrO2 co-doping, the thermal diffusion coefficient is 0.36-0.56 mm2/s, which is 20% lower than that of YSZ from room temperature to 1500 ℃，the thermal conductivities is 1.45-1.55 W/(m?K), which is 18% lower than that of YSZ from room temperature to 1500 ℃，2EYNYSZ material has single phase structure, and 1500 ℃ heat-treatment does not occur phase transition within 100 h.

Rock masses are typically anisotropic discontinuous materials composed of joints, fractures, and interlayers. Many instability and failure cases in geotechnical engineering have been induced by the expansion and transfixion of cracks in the rock masses. The mechanical properties and fracture characteristics of joints usually determine the bearing capacities and fracture modes of rocks. As such, the investigation of the crack initiation and expansion law, strength, and deformation characteristics of fractured rock masses has great significance. In this study, the rock samples investigated were taken from deep zones in the Sanshandao gold mine, where the surrounding rock masses are fractured and the maintenance costs of deep tunnels are very high. To investigate the mechanical behaviors of fractured rock masses, uniaxial compression experiments were conducted on granite samples with pre-existing cracks. First, cracks were generated in cylindrical rock samples by a water-jet cutter. Then, the rock samples with cracks and intact samples were compressively tested using a rigid testing machine (GAW2000) to determine their strength characteristics, crack initiation rules, and failure modes. The experimental results show that the uniaxial compressive strength (UCS) values of granite samples with pre-existing cracks are lower than those of intact samples, and the extent of the UCS reduction is closely related to β, i.e., the angle between the pre-existing crack and the direction of the external load. When β is 75°, the UCS values of the samples are at their minimum, and the reduction rate reaches 84.5%. These experimental results are in good agreement with the numerical solution of the maximum distortion energy criterion. Pre-existing cracks change the failure modes of rocks. With an increase in the dip angle of a pre-existing crack, the crack initiation angle increases monotonically, and the failure mode of the rock sample changes from shear failure to tensile failure. The mechanical properties and crack initiation characteristics of real-fracture rock samples can more accurately reveal the strength characteristics and failure modes of fissured granite, and thereby provide a scientific basis for the support of fractured rock masses and geotechnical engineering design.

BACKGROUND Overgrowth diamond refers to the product of thick CVD synthetic diamond layer grown on a natural diamond with the whole appearance. Since overgrown diamonds contain information on the nitrogen impurities of natural diamonds, traditional synthetic diamond screening methods and testing procedures are no longer applicable to the detection of overgrown diamonds. OBJECTIVE To gemologically test an overgrowth diamond sample in order to investigate the full identification features and to propose the optimum detection strategy. METHODS Minspecting, screening instruments testing, UV-Vis/FTIR/PL spectra examinations, as well as fluorescence and phosphorescence observation under deep UV excitation are carried out. RESULTS The sample cannot be identified by traditional microscopic observation, diamond instrument screening, and infrared spectra measurement. The sample has a distinct boundary that is separated by different luminescence under DiamondViewTM. The upper layer displays red fluorescence and greenish blue phosphorescence, while the lower layer shows deep blue fluorescence and no phosphorescence. Infrared spectroscopic analysis shows that the upper layer is type Ⅱa and the lower layer is type Ⅰa diamond. The UV-Vis absorption and photoluminescence spectra confirmed the coexistence of N3 centers and a high level of[Si-V]- defects in the diamond. CONCLUSIONS It was confirmed that the lower part of the sample is natural and the upper part of the sample is CVD synthetic layer with a thickness around 740μm. The first overgrowth diamond reported domestically was compared to the same type of samples reported abroad. The existence of a boundary are missing and the thickness of the CVD layer increased significantly in this sample. The innovative application of the multidirectional luminescence imaging and spectroscopic analyses is the key to laboratory testing of overgrowth diamond.