Search

ObjectiveThe study investigated the evolutionary associations of drug resistance in main serotype Salmonella enteritidis （S. enteritidis）in Jiamusi City， to provide a scientific basis for clinical treatment.MethodsS. enteritidis was isolated from food and patients with diarrhea. Serological typing was performed. Resistance against 16 antibiotics was investigated using the microbroth dilution method. Genes associated with antimicrobial resistance were analyzed by PCR， and the evolutionary relationships between strains were analyzed by pulsed-field gel electrophoresis （PFGE）.ResultsOf the 108 Salmonella strains identified， 55 （50.93%） were found to be S. enteritidis. Forty-nine （89.09%） of these strains showed antimicrobial resistance， with 33 strains （67.35%， 33/49） found to be resistant to multiple antibiotics. The highest resistance was against nalidixic acid and ampicillin， with 81.82% and 60.00% resistance， respectively. Homologies between 37% and 100% were observed among the 55 S. enteritidis strains.ConclusionHigh levels of multi-antimicrobial resistance were found in S. enteritidis in Jiamusi. A close relationship was found between， pathogen strain and drug resistance. S. enteritidis in the diarrhea of infected patients can cross-infect food. As S. enteritidis shows significant drug resistance in this region， it is of great importance to develop targeted diagnostic and treatment programs， as well as prevention and control measures， to ensure the safety and maintenance of public health.

ObjectiveTo provide scientific basis for formulating prevention and control measures， the epidemiological factors of an foodborne gastroenteritis outbreak caused by Norovirus in a university of Wuhan in December 2021 were analyzed.MethodsThe field epidemiological method was used to investigate. The risk factors leading to the event were studied by descriptive epidemiological method and case-control method. Anal swabs and environmental samples were collected for pathogenic detection.ResultsOne hundred and thirty nine cases were identified， with an attack rate of 0.43%. The main clinical symptoms were nausea， vomiting and diarrhea. Eating H Street pickles was a risk exposure factor （OR=15.96， 95%CI： 2.32-383.9）. Twenty nine cases and 5 food practitioners were positive for Norovirus GⅡ.ConclusionThe foodborne gastroenteritis outbreak was caused by Norovirus GⅡ. In the season of high incidence of Norovirus infection， the monitoring of norovirus infection should be strengthened in schools and food practitioners， and health education of food hygiene should be carried out actively to prevent the outbreak of intestinal pathogen infection.

ObjectiveTo determine the association of dietary diversity and dietary pattern with cognitive function in elderly in community.MethodsA cross-sectional study was conducted to randomly select 143 elderly people over 65 years old in Wangdingdi Community of Tianjin. Self-designed questionnaire was used and then dietary diversity index was calculated. Wechsler Adult Intelligence Scale-Chinese Revised （WAIS-RC） was used to measure intelligence quotient （IQ） to assess cognitive function. Factor analysis and multivariate linear regression model were used to extract dietary patterns and determine the association of dietary diversity index and dietary patterns with cognitive function， respectively.ResultsFactor analysis revealed four dietary patterns， which were meat and cereal dietary pattern， fish and poultry milk dietary pattern， bean vegetable dietary pattern， and egg dietary pattern. Multivariate linear regression showed that egg dietary pattern was significantly associated with performance IQ （P0.05）.ConclusionEgg dietary pattern may protect cognitive function in the elderly. Therefore， the elderly should increase the intake of eggs in daily diet.

ObjectiveThe present study was undertaken to identify the serotypes and drug resistance profiles of Salmonella recovered from diarrheal patients in Anhui Province, and provide evidence for the diagnosis and treatment of foodborne diseases caused by Salmonella.MethodsFrom 2015 to 2020, 980 Salmonella were collected from 16 Centers for Disease Control and Prevention in Anhui Province. The strains were serotyped, and the drug susceptibility profile was studied by micro-broth dilution method.ResultsNine hundred and eighty Salmonella included 82 serotypes with a predominance of Salmonella enteritidis 31.43% (308/980), Salmonella typhimurium 26.22% (257/980), Salmonella agona 7.35% (72/980), Salmonella thompson 6.63% (65/980) and Salmonella london 3.37% (33/980). The highest resistance were ampicillin (AMP), nalidixin (NAL), tetracycline (TET) and ampicillin/sulbactam (AMS), which were 62.35%, 47.96%, 47.86%, 43.16%, respectively. The resistance rates to AMP, AMS, NAL, cefoxitin (CFX), and azithromycin (AZM) showed a significant upward trend (P

ObjectiveTo understand the drug resistance and homology of diarrheagenic Escherichia coli (E. coli), from food in Jiamusi, and to provide basis for effective control of food borne diseases and clinical treatment.MethodsMultiple polymerase chain reaction (PCR) was used to detect diarrheagenic E. coli in food. Drug resistance of diarrheagenic E. coli isolated from food in Jiamusi was analyzed by Sensitire microbial sensitivity analysis system, and the homology of isolates was analyzed by pulsed-field gel electrophoresis (PFGE).ResultsFifty one strains of diarrheagenic E. coli were isolated from 1 320 samples with a total positive rate of 3.86%. Twenty one strains of them were resistant to drugs (41.18%), among which 3 were single drug-resistant and other 18 were multi drug-resistant. A total of 49 different PFGE fingerprints were obtained from 51 strains of diarrheagenic E. coli, and the homology was 38.6%~100%.ConclusionThe multi drug-resistant of diarrheagenic E. coli from food in Jiamusi is obvious. The homology is polymorphic.

Statin therapy is a cornerstone in the treatment of systemic vascular diseases. However, statins have failed to translate as therapeutics for pulmonary vascular disease. Early pulmonary vascular disease in the setting of congenital heart disease (CHD) is characterized by endothelial dysfunction, which precedes the more advanced stages of vascular remodeling. These features make CHD an ideal cohort in which to re-evaluate the potential pulmonary vascular benefits of statins, with a focus on endothelial biology. However, it is critical that the full gamut of the pleiotropic effects of statins in the endothelium are uncovered. The purpose of this investigation was to evaluate the therapeutic potential of simvastatin for children with CHD and pulmonary over-circulation, and examine mechanisms of simvastatin action on the endothelium. Our data demonstrate that daily simvastatin treatment preserves endothelial function in our shunt lamb model of pulmonary over-circulation. Further, using pulmonary arterial endothelial cells (PAECs) isolated from Shunt and control lambs, we identified a new mechanism of statin action mediated by increased expression of the endogenous Akt1 inhibitor, C-terminal modifying protein (CTMP). Increases in CTMP were able to decrease the Akt1-mediated mitochondrial redistribution of endothelial nitric oxide synthase (eNOS) which correlated with increased enzymatic coupling, identified by increases in NO generation and decreases in NOS-derived superoxide. Together our data identify a new mechanism by which simvastatin enhances NO signaling in the pulmonary endothelium and identify CTMP as a potential therapeutic target to prevent the endothelial dysfunction that occurs in children born with CHD resulting in pulmonary over-circulation.

This is paper is taking the design of wildlife corridor from Mingshui to Hami section of national expressway from Beijing to Urumqi as an example. From January 2015 to May 2018, the project team monitored and studied the wildlife utilization of 13 corridors along the route from Mingshui to Hami. High utilization rate was detected with 3 channels.

In this paper, the author is taking the protection of wildlife for the purpose for researching. The authors are taking the protection of animals and plants specific measures to research in the construction management. Taking the protection of wild animals and plants along the highway in the Xinjiang desert Gobi area as a research object, conservation research was carried out, and protection suggestions and measures were put forward.

The coalescence-induced droplet jumping on superhydrophobic surfaces has attracted considerable attention over the past several years. Most of the studies on droplet jumping mainly focus the droplet jumping on almost flat surfaces or ignore the effect of the microstructure. However, the microstructure often exists on superhydrophobic surfaces, and this effect remains little noticed and poorly understood. In this work, a simulation is carried out to investigate the effect of microstructure on droplet jumping. The microstructure with a similar scale to the jumping droplet on superhydrophobic will affect the jumping direction. The microstructure will improve the jumping velocity and change the jumping direction of the droplet. This work will provide effective guidelines for the design of functional SHSs with controlled and enhanced droplet jumping for a wide range of industrial applications.

Droplets on the superhydrophobic surface can fall off the surface spontaneously, which greatly promote dropwise condensation. This study considers a continuous droplet condensation process including droplet growth and droplet jumping. A droplet growth model considered NCG is developed and droplet jumping is simulated using VOF (Volume Of Fluid) model. Al–based superhydrophobic surfaces are prepared using chemical deposition and etching method. The Al-based superhydrophobic surface has a contact angle of 157°±1° and a rolling angle of 2°±1°. An observation experiment is designed to observe droplet jumping on superhydrophobic surface using a high– speed camera system. The result of droplet growth model shows a good match with experimental data in mid-term of droplet growth. Fordroplet jumping, simulation and experiment results show that droplet jumping of different diameter hasa universality in a non–dimensional form. The jumping process can be divided into 3 stages and droplet vibration is observed.