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Background Comparison of diagnostic efficiency of four ELISA kits for determining HBsAg. Methods Collecting 348 serum samples from clinical laboratory of Renmin Hospital of Wuhan University without specific gender and age requirements, determining HBsAg by the four ELISA kits, simultaneously. Confirmatory test was carried out when the initial results are positive. Results 329 out of 348 samples results were consistent (94.5%), 152 out of 329 were positive, and 177 out of 329 were negative, and all the positive results can be confirmed; 19 samples were not consistent (5.5%), and only 1 sample was confirmed by the confirmatory test. After calculation, the diagnostic efficiencies of the four ELISA kits (Beijing Wantai, Xiamen Xinchuang, Zhuhai Lizhu, and Shanghai Kehua) were 98.6%, 98.0%, 99.7%, and 97.4%, respectively, the sensitivities were 100%, 99.4%, 99.4%, and 100%, respectively, the specificities were 97.5%, 96.9%, 100%, and 95.4%, respectively, the false positive rates were 2.5%, 3.1%, 0, and 4.6%, respectively, and the false negative rates were 0, 0.6%, 0.6%, and 0, respectively. All the initial absorbencies of the false positive samples were less than 1.0. Conclusion The diagnostic efficiency of Lizhu is the highest, while Kehua is the lowest. False positive or false negative results would take place in any of the kits, an HBsAg confirmatory test is essential for initially positive samples, especially with absorbance

infections. Therefore, rapid detection of respiratory pathogens is necessary for the correct and appropriate treatment of ARI. Objectives: To obtain a general understanding of the pathogenic spectrum of ARI for children and provide basis for clinical infection control and pathogenic detection. Materials and Methods: Serum specimens from 4550 child victims with ARI symptoms were collected and detected for IgM antibodies of nine common pathogens using the indirect immunofluorescence assay (IFA). Results: Of the 4550 child victims, 3660 were antibody positive (80.4%), with detectable rates of 67.3%, 90.9%, 92.4% and 91.4% for the ≤ 1, 1 6 age groups respectively (P < 0.05). Except for Coxiellaburneti (COX), the difference in the detectable rates of other pathogens between the groups was statistically significant. Of the nine pathogens, Mycoplasma pneumoniae (MP) had the highest detectable rate, followed by influenza B virus (FluB), respiratory syncytial virus (RSV), parainfluenza virus (PIV), adenovirus (ADV), influenza A virus (FluA), Legionella pneumophila sero group I (LPN-I), Coxiellaburneti (COX) and Chlamydia pneumoniae (CPn); and 718 suffered mixed infections (81.4%), typically of two pathogens (MP + FLuB). Conclusions: MP, FluB and RSV are the principal contributors to ARI in the Hubei area, with mixed infections, typically of MP and FluB, being the most common pathogens statistically significant between different age groups.

BACKGROUND: Comparison of diagnostic efficiency of four ELISA kits for determining HBsAg. METHODS: Collecting 348 serum samples from clinical laboratory of Renmin Hospital of Wuhan University without specific gender and age requirements, determining HBsAg by the four ELISA kits, simultaneously. Confirmatory test was carried out when the initial results are positive. RESULTS: 329 out of 348 samples results were consistent (94.5%), 152 out of 329 were positive, and 177 out of 329 were negative, and all the positive results can be confirmed; 19 samples were not consistent (5.5%), and only 1 sample was confirmed by the confirmatory test. After calculation, the diagnostic efficiencies of the four ELISA kits (Beijing Wantai, Xiamen Xinchuang, Zhuhai Lizhu, and Shanghai Kehua) were 98.6%, 98.0%, 99.7%, and 97.4%, respectively, the sensitivities were 100%, 99.4%, 99.4%, and 100%, respectively, the specificities were 97.5%, 96.9%, 100%, and 95.4%, respectively, the false positive rates were 2.5%, 3.1%, 0, and 4.6%, respectively, and the false negative rates were 0, 0.6%, 0.6%, and 0, respectively. All the initial absorbencies of the false positive samples were less than 1.0. CONCLUSION: The diagnostic efficiency of Lizhu is the highest, while Kehua is the lowest. False positive or false negative results would take place in any of the kits, an HBsAg confirmatory test is essential for initially positive samples, especially with absorbance